SitesBLAST

W22 (≠ N16) mutation to A: Decreased heat stability.
E26 (≠ S20) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ I24) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ K28) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (≠ T219) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (≠ N232) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E269) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8qeuA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with ornithine (see paper)
44% identity, 98% coverage: 1:301/306 of query aligns to 1:302/304 of 8qeuA

query
sites
8qeuA

M

L

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K

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Y

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A

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D

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A

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L

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T

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K

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A

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G

G

D

D

G

G

N

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N
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N

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M

A

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W

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E

A

L

A

A

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Y

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A

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A

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M

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A

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L

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A

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binding L-ornithine: L125 (= L124), N161 (= N160), D222 (= D221), S226 (= S225), M227 (= M226), L263 (= L262)

8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
42% identity, 98% coverage: 1:301/306 of query aligns to 1:295/297 of 8qevA

query
sites
8qevA

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x
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T

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A

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L

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D

V

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W

W

T

A

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A

M

-

G

-

Y

-

E

-

A

-

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-

N

-

E

E

A

A

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K

A

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L

binding phosphoric acid mono(formamide)ester: S52 (= S51), M53 (≠ T52), R54 (= R53), T55 (= T54), R103 (= R102), C255 (= C261), L256 (= L262), R283 (= R289)

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
41% identity, 97% coverage: 2:297/306 of query aligns to 12:305/316 of Q81M99

query
sites
Q81M99

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A

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N

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G

G

K

I

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K

V

L

A

A

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Y

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V

G

G

D

D

G

G

N

N

N
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N

M

V

A

C

N

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W

L

L

L

Q

L

A

A

A

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A

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Y

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N

E

E

K

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I

-

V

-

K

A

A

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G

A

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H

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T

P

G

G

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C

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F

L

A

H

N

N

P

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A

A

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D

E

A

A

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D

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W

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S

M
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M

G

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Y

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E

E

A

G

E

E

N

E

E

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A

-

R

K

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K

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Q

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A

K

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A

A

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C

L
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L

P

P

A

A

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R

R

G

E

E

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M

T

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D

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I

I

D

D

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P

P

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F

D

E

E

Q

A

A

E

G

N

N

R
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R

M

L

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H

V

A

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Q

K

K

A

A

L

L

M

L

STRT 57:60 (= STRT 51:54) binding carbamoyl phosphate
Q84 (= Q78) binding carbamoyl phosphate
R108 (= R102) binding carbamoyl phosphate
HPCQ 135:138 (= HPCQ 129:132) binding carbamoyl phosphate
N166 (= N160) binding L-ornithine
D230 (= D221) binding L-ornithine
SM 234:235 (= SM 225:226) binding L-ornithine
CL 269:270 (= CL 261:262) binding carbamoyl phosphate
R297 (= R289) binding carbamoyl phosphate

P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 98% coverage: 1:301/306 of query aligns to 2:304/307 of P9WIT9

query
sites
P9WIT9

M

I

K

R

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F

D

A

D

D

D

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A

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-

K

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-

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Q

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K

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A

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A

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M

A

A

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A

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N

N

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H
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H

P
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P

C
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Q
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L

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A

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K

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L

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Y

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F

G

G

D

D

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G

-

A

N

N

N
|
N

M

M

A

A

N

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L

L

L

Q

L

A

G

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-

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A

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D

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W

W

T

T

S
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S

M
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M

G

G

Y

Q

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E

A

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N

G

E

L

A

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K

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A

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K

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A

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C
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C

L
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L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

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W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

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H

V

A

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Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

STRT 50:53 (= STRT 51:54) binding carbamoyl phosphate
Q77 (= Q78) binding carbamoyl phosphate
R101 (= R102) binding carbamoyl phosphate
HPCQ 128:131 (= HPCQ 129:132) binding carbamoyl phosphate
N160 (= N160) binding L-ornithine
D224 (= D221) binding L-ornithine
SM 228:229 (= SM 225:226) binding L-ornithine
CL 264:265 (= CL 261:262) binding carbamoyl phosphate
R292 (= R289) binding carbamoyl phosphate

7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:305/308 of 7nouA

query
sites
7nouA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

S

A

P

D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I
|
I

F

F

E

D

K

K

A

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

V

V

H

V

L

V

T

D

T

S

G

G

D

S

S

T

Q

Q

L
|
L

G

G

R

R

A
x
D

E
|
E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V
|
V

I

L

S

S

R

R

M
x
Y

T

V

D

D

L

A

V

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D221), C265 (= C261), R293 (= R289)
binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (= I46), T52 (= T52), R53 (= R53), R53 (= R53), F56 (≠ V56), F56 (≠ V56), L79 (= L79), D82 (≠ A82), E83 (= E83), V91 (= V91), Y95 (≠ M95), L266 (= L262), R293 (= R289)

7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:305/308 of 7nosA

query
sites
7nosA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

S

A

P

D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I
|
I

F

F

E

D

K

K

A

N

S

S

T

T

R

R

T

T

R

R

V

F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

V

V

H

V

L

V

T

D

T

S

G

G

D

S

S

T

Q

Q

L

L

G

G

R

R

A
x
D

E
|
E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V
|
V

I
x
L

S

S

R

R

M

Y

T

V

D

D

L

A

V

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D221), C265 (= C261), R293 (= R289)
binding 4-bromanyl-6-(trifluoromethyl)-1~{H}-benzimidazole: I46 (= I46), D82 (≠ A82), E83 (= E83), V91 (= V91), L92 (≠ I92)

7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:305/308 of 7norA

query
sites
7norA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

S

A

P

D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I

I

F

F

E

D

K

K

A

N

S

S

T
|
T

R

R

T

T

R

R

V
x
F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

V

V

H

V

L

V

T

D

T

S

G

G

D

S

S
x
T

Q

Q

L
|
L

G

G

R

R

A

D

E

E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V

V

I

L

S

S

R

R

M

Y

T

V

D

D

L

A

V

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D221), C265 (= C261), R293 (= R289)
binding 2-fluoro-4-hydroxybenzonitrile: T52 (= T52), F56 (≠ V56), T77 (≠ S77), L79 (= L79)

7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:305/308 of 7nnyA

query
sites
7nnyA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

S

A

P

D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I

I

F

F

E

D

K

K

A

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

V

V

H

V

L

V

T

D

T

S

G

G

D

S

S

T

Q

Q

L

L

G

G

R

R

A

D

E
|
E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V
|
V

I

L

S

S

R

R

M
x
Y

T

V

D

D

L

A

V

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D221), C265 (= C261), R293 (= R289)
binding 1-naphthol: T52 (= T52), R53 (= R53), F56 (≠ V56), E83 (= E83), V91 (= V91), Y95 (≠ M95)

7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:305/308 of 7nnwA

query
sites
7nnwA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

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A

P

D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I
|
I

F

F

E

D

K

K

A

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S

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T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

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V

H

V

L

V

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D

T

S

G

G

D

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Q

L
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L

G

G

R

R

A

D

E

E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V

V

I
x
L

S

S

R

R

M
x
Y

T

V

D

D

L

A

V

I

M

V

I

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R
|
R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D221), C265 (= C261), R293 (= R289)
binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (= I46), T52 (= T52), R53 (= R53), F56 (≠ V56), L79 (= L79), L92 (≠ I92), Y95 (≠ M95)

7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:305/308 of 7nnvA

query
sites
7nnvA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

S

A

P

D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I

I

F

F

E

D

K

K

A

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

V

V

H

V

L

V

T

D

T

S

G

G

D

S

S

T

Q

Q

L

L

G

G

R

R

A

D

E

E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V

V

I

L

S

S

R

R

M

Y

T

V

D

D

L

A

V

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

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W

L

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D221), C265 (= C261), R293 (= R289)
binding phosphoric acid mono(formamide)ester: S51 (= S51), T52 (= T52), R53 (= R53), T54 (= T54), R102 (= R102), H129 (= H129), C265 (= C261), L266 (= L262), R293 (= R289)

2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
42% identity, 98% coverage: 1:301/306 of query aligns to 2:304/307 of 2i6uA

query
sites
2i6uA

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

L

L

G

S

A

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D

A

E

E

Y

Q

N

A

Y

E

L

V

F

L

S

E

R

L

A

A

I

E

I

L

K

K

G

-

K

-

F

-

K

K

S

D

Y

P

E

V

K

S

Y

R

H

R

P

P

L

L

-

Q

A

G

D

P

R

R

T

G

L

V

A

A

M

V

I

I

F

F

E

D

K

K

A

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

E

E

A

L

G

G

M

I

Y

A

Q

Q

M

L

G

G

S

H

V

A

V

V

H

V

L

V

T

D

T

S

G

G

D

S

S

T

Q

Q

L

L

G

G

R
|
R

A

D

E

E

P

T

I

L

E

Q

D

D

S

T

A

A

R

K

V

V

I

L

S

S

R

R

M

Y

T

V

D

D

L

A

V

I

M

V

I

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R
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R

T

T

F

F

G

G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

R

T

V

V

P

P

V

V

I

I

N

N

G

A

L
|
L

T

S

N

D

E

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

I

L

F

Q

T

T

Y

I

I

A

E

E

H

R

R

K

G

G

S

A

I

L

T

R

G

G

K

L

T

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

N
|
N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

G

G

F

I

T

H

V

V

H

T

V

V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S
|
S

M
|
M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R52 (= R53), T53 (= T54), R80 (= R81), R101 (= R102), H128 (= H129), Q131 (= Q132), D224 (= D221), C264 (= C261), R292 (= R289)
binding phosphoric acid mono(formamide)ester: S50 (= S51), T51 (= T52), R52 (= R53), T53 (= T54), R101 (= R102), C264 (= C261), L265 (= L262), R292 (= R289)
binding norvaline: L123 (= L124), N160 (= N160), D224 (= D221), S228 (= S225), M229 (= M226)

4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
41% identity, 97% coverage: 2:297/306 of query aligns to 8:301/307 of 4nf2A

query
sites
4nf2A

K

K

H

D

Y

L

L

L

Q

T

F

L

A

E

D

E

L

L

G

T

A

Q

D

E

E

E

Y

I

N

I

Y

S

L

L

F

I

S

E

R

F

A

A

A

I

I

Y

I

L

K

K

G

K

K

N

F

-

K

-

S

-

Y

-

E

-

K

K

Y

Q

H

E

P

P

L

L

A

L

D

Q

-

G

R

K

T

I

L

L

A

G

M

L

I

I

F

F

E

D

K

K

A

H

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

V

S

S

F

F

E

E

A

A

G

G

M

M

Y

V

Q

Q

M

L

G

G

S

H

V

G

V

M

H

F

L

L

T

N

T

G

G

K

D

E

S

M

Q

Q

L

M

G

Q

R
|
R

A

G

E

E

P

T

I

V

E

S

D

D

S

T

A

A

R

K

V

V

I

L

S

S

R

H

M

Y

T

I

D

D

L

G

V

I

M

M

I

I

R
|
R

T

T

F

F

G

S

Q

H

D

A

K

D

I

V

A

E

R

E

F

L

A

A

E

K

F

E

S

S

R

S

V

I

P

P

V

V

I

I

N

N

G

G

L
|
L

T

T

N

D

E

D

F

H

H
|
H

P

P

C

C

Q
|
Q

I

A

M

L

A

A

D

D

I

L

F

M

T

T

Y

I

I

Y

E

E

H

E

R

T

G

N

S

T

I

F

T

K

G

G

K

I

T

K

V

L

A

A

W

Y

V

V

G

G

D

D

G

G

N

N

N
|
N

M

V

A

C

N

H

T

S

W

L

L

L

Q

L

A

A

A

S

D

A

L

K

L

V

G

G

F

M

T

H

V

M

H

T

V

V

S

A

T

T

P

P

G

V

G

G

Y

Y

E

R

V

P

D

N

E

E

K

E

L

I

-

V

-

K

A

A

F

L

G

A

G

I

K

A

P

K

H

E

R

T

P

G

G

A

C

E

Y

I

K

E

V

I

F

L

A

H

N

N

P

P

M

E

D

L

A

A

C

V

R

N

D

E

A

A

D

D

L

F

V

I

T

Y

T

T

D
|
D

V

V

W

W

T

M

S
|
S

M
|
M

G

G

Y

Q

E

E

A

G

E

E

N

E

E

E

A

-

R

K

R

Y

K

T

A

L

F

F

A

Q

D

P

W

Y

C

Q

V

I

D

N

A

K

E

E

M

L

M

V

A

K

V

H

A

A

K

K

P

Q

D

T

A

Y

L

H

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

E

E

E

V

V

M

T

A

G

D

E

V

I

I

I

D

D

G

G

P

P

Q

Q

S

S

V

I

V

V

W

F

D

E

E

Q

A

A

E

G

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

active site: R55 (= R53), T56 (= T54), R83 (= R81), R104 (= R102), H131 (= H129), Q134 (= Q132), D226 (= D221), C265 (= C261), R293 (= R289)
binding phosphoric acid mono(formamide)ester: S53 (= S51), T54 (= T52), R55 (= R53), T56 (= T54), R104 (= R102), H131 (= H129), Q134 (= Q132), C265 (= C261), L266 (= L262), R293 (= R289)
binding norvaline: L126 (= L124), N162 (= N160), D226 (= D221), S230 (= S225), M231 (= M226)

7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
42% identity, 98% coverage: 1:301/306 of query aligns to 3:302/305 of 7np0A

query
sites
7np0A

M

I

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Y

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A

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L

A

A

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E

I

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K

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-

K

-

F

-

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P

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L

-

Q

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A

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K

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F

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E

A

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A

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Q

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-

H

H

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E

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V

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x
Y

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R

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G

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A

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A

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A

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H

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I

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A

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G

K

L

T

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L

A

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Y

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F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

L

L

Q

L

A

G

D

V

L

T

L

A

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G

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V

S

A

T

A

P

P

G

E

G

G

Y

F

E

L

V

P

D

D

E

P

K

S

L

V

A

R

F

A

G

A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

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D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

M

L

H

H

V

A

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Q

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K

A

A

L

L

M

L

E

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L

L

active site: R99 (= R102), H126 (= H129), Q129 (= Q132), D222 (= D221), C262 (= C261), R290 (= R289)
binding p-nitrophenylboronic acid: E80 (= E83), V88 (= V91), Y92 (≠ M95)

7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
41% identity, 98% coverage: 1:301/306 of query aligns to 2:294/297 of 7nnzB

query
sites
7nnzB

M

I

K

R

H

H

Y

F

L

L

Q

R

F

D

A

D

D

D

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L

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A

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N

A

Y

E

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L

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E

R

L

A

A

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E

I

L

K

K

G

-

K

-

F

-

K

K

S

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Y

P

E

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K

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Y

R

H

R

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P

L

L

-

Q

A

G

D

P

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R

T

G

L

V

A

A

M

V

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F

E

D

K

K

A

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|
T

R
|
R

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x
F

S

S

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F

E

E

A

L

G

G

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A

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M

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A

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V

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L

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V

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T

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A

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A

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M

A

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V

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I

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N

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A

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H

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C

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I

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G

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L

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L

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G

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D

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G

-

A

N

N

M

M

A

A

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L

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L

A

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D

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L

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L

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-

D

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-

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A

A

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P

A

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A

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G

A

A

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D

L

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D
|
D

V

T

W

W

T

T

S

-

M

-

G

-

Y

-

E

-

A

-

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N

-

E

-

A

-

R

R

R

V

K

K

A

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F

A

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C

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N

A

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A

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L

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K

D

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S

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D

A

A

L

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M

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H

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C
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C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

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M

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D

A

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V

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M

D

D

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G

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P

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A

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E

A

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N

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R

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L

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H

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A

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Q

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K

A

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L

L

M

L

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L

L

active site: R101 (= R102), H128 (= H129), Q131 (= Q132), D224 (= D221), C254 (= C261), R282 (= R289)
binding 5-methyl-4-phenyl-1,3-thiazol-2-amine: T51 (= T52), R52 (= R53), F55 (≠ V56)

7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
41% identity, 98% coverage: 1:301/306 of query aligns to 3:299/302 of 7novA

query
sites
7novA

M

I

K

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H

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L

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D

A

D

D

D

L

L

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Y

E

L

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L

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L

A

A

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E

I

L

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K

G

-

K

-

F

-

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K

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Y

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Y

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P

L

L

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A

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L

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I

I

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F

E

D

K

K

A

N

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T

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|
R

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x
F

S

S

F

F

E

E

A

L

G

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A

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Q

M

L

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S

-

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-

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H

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A

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D

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-

L

-

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-

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R

A

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E
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E

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T

A

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V

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x
Y

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D

D

L

A

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M

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I

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R
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R

T

T

F

F

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G

Q

Q

D

E

K

R

I

L

A

D

R

A

F

M

A

A

E

S

F

V

S

A

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T

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V

P

P

V

V

I

I

N

N

G

A

L

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N

D

E

E

F

F

H
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H

P

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C

Q
|
Q

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A

A

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D

I

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F

Q

T

T

Y

I

I

A

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A

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G

G

K

L

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R

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L

A

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Y

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F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

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L

L

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L

A

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F

A

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A

K

E

P

R

H

R

R

A

P

Q

-

D

-

T

-

G

G

A

C

S

Y

V

K

T

V

V

F

T

A

A

N

D

P

A

M

H

D

A

A

A

C

A

R

A

D

G

A

A

D

D

L

V

V

L

T

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Y

Q

E

E

A

N

E

D

N

G

E

L

A

D

R

R

R

V

K

K

A

P

F

F

A

R

D

P

W

F

C

Q

V

L

D

N

A

S

E

R

M

L

A

A

V

L

A

A

K

D

P

S

D

D

A

A

L

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

M

T

A

D

D

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

W

W

D

D

E

E

A
|
A

E

E

N

N

R
|
R

M

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

E

V

Y

W

L

L

active site: R96 (= R102), H123 (= H129), Q126 (= Q132), D219 (= D221), C259 (= C261), R287 (= R289)
binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R53), F56 (≠ V56), E77 (= E83), V85 (= V91), Y89 (≠ M95), L260 (= L262), A284 (= A286), R287 (= R289)

P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
39% identity, 97% coverage: 5:301/306 of query aligns to 43:342/354 of P00481

query
sites
P00481

L

L

Q

T

F

L

A

K

D

N

L

F

G

T

A

G

D

E

E

E

Y

I

N

Q

Y

Y

L

M

F

L

S

W

R

L

A

S

A

A

I

D

I

L

K

K

G

F

K

R

F

I

K

K

S

Q

Y

K

E

G

K

E

Y

Y

H

L

P

P

L

L

A

L

D

Q

-

G

R

K

T

S

L

L

A

G

M

M

I

I

F

F

E

E

K

K

A

R

S

S

T

T

R
|
R

T

T

R

R

V

L

S

S

F

T

E

E

A

T

G

G

M

F

Y

A

Q

L

M

L

G

G

S

H

V

P

V

S

H

F

L

L

T

T

G

Q

D

D

S

I

Q

H

L

L

G

G

R

V

A

N

E

E

P

S

I

L

E

T

D

D

S

T

A

A

R

R

V

V

I

L

S

S

R

S

M

M

T

T

D

D

L

A

V

V

M

L

I

A

R

R

T

V

F

Y

G

K

Q

Q

D

S

K

D

I

L

A

D

R

I

F

L

A

A

E

K

F

E

S

A

R

T

V

I

P

P

V

I

I

V

N

N

G

G

L

L

T

S

N

D

E

L

F

Y

H

H

P

P

C

I

Q

Q

I

I

M

L

A

A

D

D

I

Y

F

L

T

T

Y

L

I

Q

E

E

H

H

R

Y

G

G

S

S

I

L

T

K

G

G

K

L

T

T

V

L

A

S

W

W

V

I

G

G

D

D

G

G

N

N

M

I

A

L

N

H

T

S

W

I

L

M

Q

M

A

S

A

A

D

A

L

K

L

F

G

G

F

M

T

H

V

L

H

Q

V

A

S

A

T

T

P

P

G

K

G

G

Y

Y

E

E

V

P

D

D

E

P

-

N

-

I

-

V

K

K

L

L

A

A

F

E

G

Q

G

Y

K

A

P

K

H

E

R

N

P

G

G

T

C

R

Y

L

K

S

V

M

F

T

A

N

N

D

P

P

M

L

D

E

A

A

C

A

R

R

D

G

A

G

D

N

L

V

V

L

T

I

T

T

D

D

V

T

W

W

T

I

S

S

M

M

G

G

Y

Q

E

E

A

D

E

E

N

K

E

K

A

K

R

R

R

L

K

Q

A

A

F

F

A

Q

D

G

W

Y

C

Q

V

V

D

T

A

M

E

K

M

T

M

A

A

K

V

V

A

A

K

A

P

S

D

D

A

W

L

T

F

F

M

L

H

H

C
|
C

L

L

P

P

A

-

H

R

R

K

G

P

E

E

V

V

M

D

A

D

D

E

V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

V

W

F

D

P

E

E

A

A

E

E

N

N

R

R

M

K

H

W

V

T

Q

I

K

M

A

A

L

V

M

M

E

V

Y

S

L

L

R92 (= R53) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
C303 (= C261) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
38% identity, 97% coverage: 5:301/306 of query aligns to 10:309/321 of 1othA

query
sites
1othA

L

L

Q

T

F

L

A

K

D

N

L

F

G

T

A

G

D

E

E

E

Y

I

N

K

Y

Y

L

M

F

L

S

W

R

L

A

S

A

A

I

D

I

L

K

K

G

F

K

R

F

I

K

K

S

Q

Y

K

E

G

K

E

Y

Y

H

L

P

P

L

L

A

L

D

Q

-

G

R

K

T

S

L

L

A

G

M

M

I

I

F

F

E

E

K

K

A

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

E

E

A

T

G

G

M

F

Y

A

Q

L

M

L

G

G

S

H

V

P

V

C

H

F

L

L

T

T

G

Q

D

D

S

I

Q

H

L

L

G

G

R
x
V

A

N

E

E

P

S

I

L

E

T

D

D

S

T

A

A

R

R

V

V

I

L

S

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M

M

T

A

D

D

L

A

V

V

M

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R
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R

T

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Y

G

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Q

Q

D

S

K

D

I

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A

D

R

T

F

L

A

A

E

K

F

E

S

A

R

S

V

I

P

P

V

I

I

I

N

N

G

G

L
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L

T

S

N

D

E

L

F

Y

H
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H

P

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Q

I

I

M

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A

A

D

D

I

Y

F

L

T

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Y

L

I

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E

E

H

H

R

Y

G

S

S

S

I

L

T

K

G

G

K

L

T

T

V

L

A

S

W

W

V

I

G

G

D

D

G

G

N

N

N
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N

M

I

A

L

N

H

T

S

W

I

L

M

Q

M

A

S

A

A

D

A

L

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L

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G

G

F

M

T

H

V

L

H

Q

V

A

S

A

T

T

P

P

G

K

G

G

Y

Y

E

E

V

P

D

D

E

A

K

S

L

V

A

T

F

K

G

L

G

A

K

E

P

Q

H

Y

-

A

-

K

R

E

P

N

G

G

C

T

Y

K

K

L

V

L

F

L

A

T

N

N

-

D

P

P

M

L

D

E

A

A

C

A

R

H

D

G

A

G

D

N

L

V

V

L

T

I

T

T

D
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D

V

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W

W

T

I

S
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S

M
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M

G

G

Y

R

E

E

A

E

E

E

N

K

E

K

A

K

R

R

R

L

K

Q

A

A

F

F

A

Q

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D

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C

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L

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-

H

R

R

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G

P

E

E

V

V

M

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A

D

D

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I

F

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Y

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S

P

P

Q

R

S

S

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L

V

V

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F

D

P

E

E

A

A

E

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N

N

R
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R

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A

A

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L

active site: R59 (= R53), T60 (= T54), V87 (≠ R81), R108 (= R102), H135 (= H129), Q138 (= Q132), D230 (= D221), C270 (= C261), R297 (= R289)
binding n-(phosphonoacetyl)-l-ornithine: S57 (= S51), T58 (= T52), R59 (= R53), T60 (= T54), R108 (= R102), L130 (= L124), H135 (= H129), N166 (= N160), D230 (= D221), S234 (= S225), M235 (= M226), C270 (= C261), L271 (= L262), R297 (= R289)

1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
38% identity, 97% coverage: 5:301/306 of query aligns to 10:309/321 of 1c9yA

query
sites
1c9yA

L

L

Q

T

F

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A

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D

N

L

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D

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P

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S

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T

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S

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E

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L

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G

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x
V

A

N

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P

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D

D

S

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A

A

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R

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R

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F

L

A

A

E

K

F

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P

V

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N

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L

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H

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Q

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R

Y

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K

G

G

K

L

T

T

V

L

A

S

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W

V

I

G

G

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D

G

G

N

N

N
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N

M

I

A

L

N

H

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W

I

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M

Q

M

A

S

A

A

D

A

L

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L

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G

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V

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P

G

K

G

G

Y

Y

E

E

V

P

D

D

E

A

K

S

L

V

A

T

F

K

G

L

G

A

K

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P

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H

Y

-

A

-

K

R

E

P

N

G

G

C

T

Y

K

K

L

V

L

F

L

A

T

N

N

-

D

P

P

M

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A

A

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D

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S

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M

G

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A

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T

M

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A

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K

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D

D

A

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L

T

F

F

M

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C

L
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L

P

P

A

-

H

R

R

K

G

P

E

E

V

V

M

D

A

D

D

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V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

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F

D

P

E

E

A

A

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N

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R

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L

active site: R59 (= R53), T60 (= T54), V87 (≠ R81), R108 (= R102), H135 (= H129), Q138 (= Q132), D230 (= D221), C270 (= C261), R297 (= R289)
binding phosphoric acid mono(formamide)ester: S57 (= S51), T58 (= T52), R59 (= R53), T60 (= T54), R108 (= R102), C270 (= C261), L271 (= L262), R297 (= R289)
binding norvaline: L130 (= L124), N166 (= N160), D230 (= D221), S234 (= S225), M235 (= M226)

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
38% identity, 97% coverage: 5:301/306 of query aligns to 43:342/354 of P00480

query
sites
P00480

L
|
L

Q

T

F

L

A
x
K

D

N

L

F

G

T

A

G

D

E

E

E

Y

I

N

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Y
|
Y

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A

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I
x
L

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Y

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G

K

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Y

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L

L

A

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-

G

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L

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I

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K

A

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S

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T

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R

T
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T

R

R

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L

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S

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G

M
x
F

Y

A

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L

M

L

G

G

S

H

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P

V

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H

F

L
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L

T

T

G

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D

D

S

I

Q

H

L

L

G

G

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V

A

N

E

E

P

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L

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x
T

D
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D

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A

A

R
|
R

V

V

I

L

S

S

R

S

M

M

T

A

D

D

L

A

V

V

M

L

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x
A

R
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R

T

V

F

Y

G

K

Q

Q

D

S

K

D

I

L

A

D

R

T

F

L

A

A

E

K

F

E

S

A

R

S

V

I

P

P

V

I

I

I

N

N

G

G

L

L

T

S

N

D

E

L

F

Y

H
|
H

P

P

C

I

Q
|
Q

I
|
I

M

L

A

A

D

D

I
x
Y

F

L

T
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T

Y
x
L

I

Q

E

E

H

H

R
x
Y

G

S

S

S

I

L

T

K

G
|
G

K

L

T

T

V

L

A

S

W

W

V

I

G
|
G

D
|
D

G

G

N

N

M

I

A
x
L

N

H

T

S

W

I

L

M

Q

M

A
x
S

A

A

D
x
A

L

K

L

F

G

G

F
x
M

T
x
H

V

L

H

Q

V

A

S

A

T

T

P
|
P

G

K

G

G

Y

Y

E

E

V
x
P

D

D

E

A

K

S

L

V

A

T

F

K

G

L

G

A

K

E

P

Q

H

Y

-

A

-

K

R

E

P

N

G

G

C

T

Y

K

K
x
L

V

L

F

L

A

T

N

N

-

D

P

P

M

L

D

E

A

A

C

A

R

H

D

G

A

G

D

N

L

V

V

L

T

I

T
|
T

D

D

V
x
T

W
|
W

T

I

S

S

M

M

G
|
G

Y
x
Q

E

E

A
x
E

E

E

N

K

E

K

A

K

R
|
R

R

L

K

Q

A

A

F

F

A

Q

D

G

W

Y

C

Q

V

V

D

T

A

M

E

K

M

T

M

A

A

K

V

V

A

A

K

A

P

S

D

D

A

W

L

T

F

F

M

L

H
|
H

C
|
C

L
|
L

P

P

A

-

H

R

R

K

G

P

E
|
E

E

E

V

V

M

D

A

D

D

E

V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

V

W

F

D

P

E

E

A

A

E

E

N

N

R
|
R

M

K

H

W

V
x
T

Q

I

K

M

A

A

L

V

M

M

E

V

Y
x
S

L

L

L43 (= L5) to F: in dbSNP:rs72554309
K46 (≠ A8) to R: in dbSNP:rs1800321
Y55 (= Y17) to D: in OTCD; late onset; dbSNP:rs72554319
L63 (≠ I25) to P: in OTCD; late onset; dbSNP:rs72554324
K88 (= K49) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
STRT 90:93 (= STRT 51:54) binding carbamoyl phosphate
G100 (= G61) to D: in OTCD; late onset; dbSNP:rs72554349
F101 (≠ M62) to L: in dbSNP:rs1133135
L111 (= L72) to P: in dbSNP:rs1800324
T125 (≠ E86) to M: in OTCD; neonatal; dbSNP:rs72554356
D126 (= D87) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
R129 (= R90) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
A140 (≠ I101) to P: in OTCD; late onset; dbSNP:rs72556260
R141 (= R102) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
H168 (= H129) binding carbamoyl phosphate
Q171 (= Q132) binding carbamoyl phosphate
I172 (= I133) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
Y176 (≠ I137) to C: in OTCD; late onset; dbSNP:rs72556283
TL 178:179 (≠ TY 139:140) natural variant: Missing (in OTCD; neonatal)
Y183 (≠ R144) to D: in OTCD; late onset; dbSNP:rs72556292
G188 (= G149) to R: in OTCD; neonatal; dbSNP:rs72556294
G195 (= G156) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
D196 (= D157) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
L201 (≠ A162) to P: in OTCD; neonatal; dbSNP:rs72558407
S207 (≠ A168) to R: in OTCD; neonatal; dbSNP:rs72558415
A209 (≠ D170) to V: in OTCD; neonatal; dbSNP:rs72558417
M213 (≠ F174) to K: in OTCD; late onset
H214 (≠ T175) to Y: in OTCD; neonatal; dbSNP:rs72558420
P220 (= P181) to A: in OTCD; late onset; dbSNP:rs72558425
P225 (≠ V186) to T: in OTCD; late onset; dbSNP:rs72558428
L244 (≠ K203) to Q: in OTCD; late onset; dbSNP:rs72558436
T262 (= T220) to K: in OTCD; mild; dbSNP:rs67333670
T264 (≠ V222) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
W265 (= W223) to L: in OTCD; mild; dbSNP:rs72558446
G269 (= G227) to E: in OTCD; neonatal; dbSNP:rs72558450
Q270 (≠ Y228) to R: in dbSNP:rs1800328
E272 (≠ A230) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
R277 (= R235) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
H302 (= H260) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
C303 (= C261) to R: in OTCD; neonatal; dbSNP:rs67468335
CL 303:304 (= CL 261:262) binding carbamoyl phosphate
E309 (= E268) natural variant: Missing (in OTCD; late onset)
R330 (= R289) binding carbamoyl phosphate
T333 (≠ V292) natural variant: T -> A
S340 (≠ Y299) to P: in OTCD; late onset; dbSNP:rs72558489

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
39 G → C: in OTCD; late onset; dbSNP:rs72554306
40 R → H: in OTCD; late onset; dbSNP:rs72554308
343 T → K: in OTCD; late onset; dbSNP:rs72558491

Query Sequence

>WP_013518269.1 NCBI__GCF_000204645.1:WP_013518269.1
MKHYLQFADLGADEYNYLFSRAAIIKGKFKSYEKYHPLADRTLAMIFEKASTRTRVSFEA
GMYQMGGSVVHLTTGDSQLGRAEPIEDSARVISRMTDLVMIRTFGQDKIARFAEFSRVPV
INGLTNEFHPCQIMADIFTYIEHRGSITGKTVAWVGDGNNMANTWLQAADLLGFTVHVST
PGGYEVDEKLAFGGKPHRPGCYKVFANPMDACRDADLVTTDVWTSMGYEAENEARRKAFA
DWCVDAEMMAVAKPDALFMHCLPAHRGEEVMADVIDGPQSVVWDEAENRMHVQKALMEYL
LLGRIA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory