SitesBLAST
Comparing WP_013520542.1 NCBI__GCF_000204645.1:WP_013520542.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
39% identity, 99% coverage: 1:253/255 of query aligns to 2:244/244 of 4nbuB
- active site: G18 (= G17), N111 (= N114), S139 (= S142), Q149 (≠ E152), Y152 (= Y155), K156 (= K159)
- binding acetoacetyl-coenzyme a: D93 (≠ F96), K98 (= K101), S139 (= S142), N146 (≠ S149), V147 (≠ S150), Q149 (≠ E152), Y152 (= Y155), F184 (≠ P187), M189 (≠ L192), K200 (≠ A209)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ G16), G18 (= G17), I19 (= I18), D38 (= D37), F39 (≠ M38), V59 (≠ C62), D60 (= D63), V61 (≠ I64), N87 (= N90), A88 (= A91), G89 (= G92), I90 (≠ W93), T137 (≠ V140), S139 (= S142), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ P187), T185 (= T188), T187 (= T190), M189 (≠ L192)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
38% identity, 98% coverage: 4:253/255 of query aligns to 6:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G13), R18 (≠ G16), G19 (= G17), I20 (= I18), D39 (= D37), R40 (≠ M38), C63 (= C62), I65 (= I64), N91 (= N90), G93 (= G92), I94 (≠ W93), V114 (≠ I113), Y155 (= Y155), K159 (= K159), I188 (≠ T188), T190 (= T190), T193 (≠ L193)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
38% identity, 98% coverage: 4:253/255 of query aligns to 3:247/247 of 4jroC
- active site: G16 (= G17), S142 (= S142), Q152 (≠ E152), Y155 (= Y155), K159 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ G15), R15 (≠ G16), G16 (= G17), I17 (= I18), N35 (≠ D37), Y36 (≠ M38), N37 (= N39), G38 (vs. gap), S39 (vs. gap), N63 (≠ D63), V64 (≠ I64), N90 (= N90), A91 (= A91), I93 (≠ W93), I113 (= I113), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (≠ T188), T190 (= T190)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
36% identity, 97% coverage: 7:253/255 of query aligns to 5:246/246 of 3osuA
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
39% identity, 96% coverage: 9:253/255 of query aligns to 4:244/244 of 1edoA
- active site: G12 (= G17), S138 (= S142), Y151 (= Y155), K155 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), S10 (≠ G15), R11 (≠ G16), I13 (= I18), N31 (vs. gap), Y32 (≠ F36), A33 (≠ D37), R34 (≠ M38), S35 (≠ N39), D59 (= D63), V60 (≠ I64), N86 (= N90), A87 (= A91), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186), I184 (≠ T188), S186 (≠ T190), M188 (≠ L192)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
35% identity, 98% coverage: 4:252/255 of query aligns to 4:258/260 of 6zzqA
- active site: G17 (= G17), S142 (= S142), Y155 (= Y155)
- binding acetoacetic acid: Q94 (≠ D94), S142 (= S142), K152 (≠ E152), Y155 (= Y155), Q196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), S16 (≠ G16), G17 (= G17), I18 (= I18), D37 (= D37), M38 (= M38), D63 (= D63), V64 (≠ I64), N90 (= N90), A91 (= A91), G92 (= G92), M140 (≠ V140), A141 (= A141), S142 (= S142), Y155 (= Y155), K159 (= K159), Y187 (≠ P187), V188 (≠ T188), T190 (= T190)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
35% identity, 98% coverage: 4:252/255 of query aligns to 5:259/261 of 6zzsD
- active site: G18 (= G17), S143 (= S142), Y156 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G14 (= G13), S17 (≠ G16), I19 (= I18), D38 (= D37), M39 (= M38), D64 (= D63), V65 (≠ I64), N91 (= N90), A92 (= A91), G93 (= G92), M141 (≠ V140), A142 (= A141), S143 (= S142), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (= G186), V189 (≠ T188), T191 (= T190), L193 (= L192)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ D94), S143 (= S142), N145 (≠ A144), K153 (≠ E152), Y156 (= Y155), Q197 (vs. gap)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
36% identity, 98% coverage: 4:252/255 of query aligns to 3:242/251 of H9XP47
- N15 (≠ G16) binding NAD(+)
- M17 (≠ I18) binding NAD(+)
- D36 (= D37) binding NAD(+)
- D60 (= D63) binding NAD(+)
- V61 (≠ I64) binding NAD(+)
- N87 (= N90) binding NAD(+)
- S138 (= S142) binding (R)-acetoin; binding (S)-acetoin
- V139 (≠ D143) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ A144) binding (R)-acetoin
- Y151 (= Y155) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K159) binding NAD(+)
- V184 (≠ T188) binding NAD(+)
- T186 (= T190) binding NAD(+)
- RDK 197:199 (≠ RDP 201:203) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
36% identity, 98% coverage: 4:252/255 of query aligns to 3:242/251 of 6xewA
- active site: G16 (= G17), S138 (= S142), Y151 (= Y155)
- binding r,3-hydroxybutan-2-one: S138 (= S142), S140 (≠ A144), Y151 (= Y155)
- binding s,3-hydroxybutan-2-one: S138 (= S142), Y151 (= Y155), S182 (≠ G186)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ G16), G16 (= G17), M17 (≠ I18), D36 (= D37), W37 (≠ M38), W37 (≠ M38), A38 (≠ N39), I59 (≠ C62), D60 (= D63), V61 (≠ I64), N87 (= N90), A88 (= A91), G89 (= G92), V110 (≠ I113), T136 (≠ V140), S138 (= S142), Y151 (= Y155), K155 (= K159), S182 (≠ G186), L183 (≠ P187), V184 (≠ T188), T186 (= T190), N187 (≠ A191), M188 (≠ L192), T189 (≠ L193)
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
36% identity, 98% coverage: 4:252/255 of query aligns to 5:244/252 of 6vspB
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
36% identity, 98% coverage: 3:253/255 of query aligns to 2:245/248 of Q9KJF1
- S15 (≠ G16) binding NAD(+)
- D36 (= D37) binding NAD(+)
- D62 (= D63) binding NAD(+)
- I63 (= I64) binding NAD(+)
- N89 (= N90) binding NAD(+)
- Y153 (= Y155) binding NAD(+)
- K157 (= K159) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
36% identity, 98% coverage: 3:253/255 of query aligns to 1:244/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), M16 (≠ I18), D35 (= D37), I36 (≠ M38), I62 (= I64), N88 (= N90), G90 (= G92), I138 (≠ V140), S140 (= S142), Y152 (= Y155), K156 (= K159), I185 (≠ T188)
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
36% identity, 98% coverage: 4:252/255 of query aligns to 3:242/251 of 6vspA
- active site: G16 (= G17), S138 (= S142), Y151 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ G16), G16 (= G17), M17 (≠ I18), D36 (= D37), W37 (≠ M38), W37 (≠ M38), A38 (≠ N39), I59 (≠ C62), D60 (= D63), V61 (≠ I64), N87 (= N90), A88 (= A91), G89 (= G92), V90 (≠ W93), V110 (≠ I113), T136 (≠ V140), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), S182 (≠ G186), L183 (≠ P187), V184 (≠ T188), T186 (= T190), N187 (≠ A191), M188 (≠ L192), T189 (≠ L193)
P73826 Acetoacetyl-CoA reductase; EC 1.1.1.36 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
36% identity, 97% coverage: 3:249/255 of query aligns to 5:236/240 of P73826
- S134 (= S142) mutation to A: 12% enzymatic activity.
- Y147 (= Y155) mutation to A: No enzymatic activity.
- K151 (= K159) mutation to A: 5% enzymatic activity.
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
34% identity, 98% coverage: 4:253/255 of query aligns to 3:243/244 of 6t77A
- active site: G16 (= G17), S138 (= S142), Y151 (= Y155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ G15), R15 (≠ G16), T37 (≠ M38), L58 (≠ C62), N59 (≠ D63), V60 (≠ I64), A87 (= A91), G88 (= G92), I89 (≠ W93)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
34% identity, 98% coverage: 4:253/255 of query aligns to 2:242/243 of 7emgB
7x5jC Acp-dependent oxoacyl reductase
36% identity, 98% coverage: 4:253/255 of query aligns to 2:253/256 of 7x5jC
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G13), S13 (≠ G15), G15 (= G17), L16 (≠ I18), N36 (≠ D37), R37 (≠ M38), N38 (= N39), D62 (= D63), V63 (≠ I64), N89 (= N90), A90 (= A91), A91 (≠ G92), V114 (≠ I113), I141 (≠ V140), Y156 (= Y155), K160 (= K159), L186 (≠ A194), G187 (= G195), V188 (= V196), R194 (≠ A200), S197 (≠ P203), S198 (≠ A204)
9febA Short-chain dehydrogenase/reductase (sdr) from thermus caliditerrae in complex with NADP
37% identity, 100% coverage: 2:255/255 of query aligns to 4:261/261 of 9febA
9fe6B Short-chain dehydrogenase/reductase (sdr) from thermus caliditerrae
37% identity, 100% coverage: 2:255/255 of query aligns to 4:261/261 of 9fe6B
4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
36% identity, 98% coverage: 4:253/255 of query aligns to 6:251/251 of 4cqlI
- active site: G19 (= G17), S146 (= S142), Y159 (= Y155), K163 (= K159)
- binding nicotinamide-adenine-dinucleotide: S18 (≠ G16), G19 (= G17), I20 (= I18), D39 (= D37), L40 (≠ M38), A64 (≠ C62), D65 (= D63), V66 (≠ I64), C93 (≠ N90), A94 (= A91), G95 (= G92), I96 (≠ W93), V116 (≠ I113), I144 (≠ V140), S146 (= S142), Y159 (= Y155), K163 (= K159), P189 (= P185), G190 (= G186), I192 (≠ T188), T194 (= T190), M196 (≠ L192)
Query Sequence
>WP_013520542.1 NCBI__GCF_000204645.1:WP_013520542.1
MQGLKNQTVIVTGGGGGIGSATCRRFAAEGAKVAVFDMNPAAAEEVAAGIRAAGGQAAAF
QCDITDRAQVDAAVAATGAQLGPVGVLVNNAGWDVFKPFVKTVPAEWDRLIAINLTGALH
MLHAVLPGMSERRSGHIVNVASDAARGGSSGEAVYSACKGGLVALSKTLAREHARHGINV
NVVCPGPTDTALLAGVAEGARDPAKLIEAFKSAIPMGRLGRPEDLASAIVFFGSDDASFI
TGQVISVSGGLTMHG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory