SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013528949.1 NCBI__GCF_000185905.1:WP_013528949.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
33% identity, 96% coverage: 15:456/459 of query aligns to 26:469/472 of P78061

query
sites
P78061

D

E

A

V

K

K

A

R

W

Y

L

L

T

E

R

R

H

Y

-

P

G

N

I

T

N

Q

E

Y

V

V

E

D

C

V

L

L

V

L

P

T

D

D

M

L

N

N

G

G

V

C

L

F

R

R

G

G

K

K

A

R

L

I

P

P

T

V

A

S

K

S

F

-

L

L

Q

K

A

K

L

L

E

E

D

-

R

K

A

G

L

C

Y

Y

L

F

P

P

S

A

S

S

A

V

F

F

L

A

V

M

S

D

I

I

D

L

G

G

R

N

Y

V

S

V

G

E

S

E

I

A

D

G

E

L

G

G

F

Q

A

E

Y

M

S

G

D

E

P

P

D

D

M

R

R

T

M

C

V

V

P

P

D

V

V

L

S

G

T

S

L

L

C

T

L

P

A

S

P

A

G

A

A

D

A

P

P

E

G

F

K

I

A

G

Y

Q

V

M

F

L

A

L

D

T

A

M

F

V

H

D

M

E

D

D

D

G

R

A

P

P

W

F

M

D

A

V

S

E

P

P

R

R

H

N

V

V

L

L

R

N

A

R

V

L

L

W

D

Q

L

Q

Y

L

A

R

K

Q

R

R

G

G

W

L

R

F

A

P

V

V

A

A

P

V

E

E

V

L

E

E

F

F

Y

Y

L

L

T

L

A

D

P

R

N

Q

P

R

D

D

P

A

D

E

R

G

P

Y

L

L

T

Q

A

P

P

P

V

C

-

A

-

P

G

G

A

T

N

D

G

D

R

R

A

-

E

N

T

T

V

Q

Q

S

H

Q

P

V

Y

Y

D

S

M

V

A

D

G

N

L

L

E

N

E

H

F

F

E

A

P

D

V

V

I

L

R

N

R

D

I

I

Y

D

D

E

Y

L

A

A

A

Q

A

L

A

Q

G

L

L

I

P

P

L

A

D

D

T

G

L

A

I

V

H

A

E

E

S

A

G

S

T

P

A

G

Q

Q

L

F

E

E

I

I

N

N

F

L

L

Y

H

H

G

T

D

D

-

N

A

V

L

L

P

E

L

A

A

C

D

D

K

D

V

A

L

L

L

A

F

L

K

K

R

R

L

L

T

V

R

R

Q

L

A

M

A

A

Q

E

Q

K

S

H

G

K

M

M

H

H

A

A

T

T

F

F

M

M

A

A

K

K

P

P

I

Y

A

E

Q

H

A

A

G

G

S

S

S

G

M

M

H
|
H

L

I

H

H

M

I

S

S

V

M

I

Q

D

N

E

N

A

R

G

G

R

E

T

N

L

V

F

L

A

S

G

D

S

A

D

E

D

G

A

E

D

D

T

S

A

P

M

L

F

L

G

K

H

K

F

M

I

L

G

A

G

G

L

M

Q

I

K

D

Y

L

V

M

P

P

E

S

I

S

M

M

P

A

L

L

F

L

A

A

P

P

N

N

V

V

N

N

S

S

F

Y

R

R

I

F

R

Q

P

P

N

G

H

M

S

Y

A

V

P

P

A

T

N

Q

I

A

E

S

W

W

S

G

H

H

D

N

N

N

R

R

S

T

C

V

G

A

L

L

R
|
R

V

I

P

P

A

C

G

G

G

D

R

R

A

H

A

N

R

H

R

R

V

V

E

E

N

Y

R

R

L

V

P

A

G

G

A

A

D

D

S

A

N

N

P

P

Y

Y

L

L

A

V

I

M

A

A

G

-

S

A

L

I

L

F

A

A

G

G

Y

I

L

L

-

H

G

G

V

L

E

D

Q

N

K

E

L

L

A

P

R

L

S

Q

P

E

E

E

A

V

S

E

G

G

N

N

A

G

Y

L

K

E

I

Q

K

E

S

G

T

L

-

P

L

F

P

P

K

I

T

R

M

Q

E

S

E

D

A

A

L

L

D

G

R

E

F

F

E

I

A

E

C

N

G

D

P

H

V

L

R

R

E

R

L

Y

L

L

G

G

E

E

D

R

F

F

F

C

Q

H

T

V

Y

Y

L

H

R

A

V

C

K

K

S

N

V

D

E

E

L

L

D

L

L

Q

F

F

Q

E

G

R

V

L

V

I

T

T

S

E

W

T

E

E

R

I

D

E

H

W

L

M

L

L

H282 (= H270) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R345) mutation to Q: Activity is impaired to 3% of wild-type.

8tfkA Glutamine synthetase (see paper)
31% identity, 92% coverage: 34:456/459 of query aligns to 24:437/440 of 8tfkA

query
sites
8tfkA

D

D

M

T

N

L

G

G

V

I

L

I

R

K

G

S

K

W

A

A

L

I

P

P

T

A

A

E

K

Q

F

L

L

E

Q

E

A

A

L

F

E

E

D

N

R

G

A

V

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

Q

G

G

F

F

A

T

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

K

M

L

V

A

P

L

D

D

V

P

S

S

T

T

L

F

C

R

L

I

A

L

P

P

G

W

-

R

-

P

A

A

A

T

P

G

G

A

K

V

A

A

Y

R

V

I

F

L

A

G

D

D

A

V

F

Y

H

L

M

P

D

D

D

G

R

N

P

P

W

F

M

K

A

G

S

D

P

P

R

R

H

Y

V

V

L

L

R

K

A

T

V

A

L

I

D

K

L

E

Y

A

A

E

K

K

R

M

G

G

W

F

R

S

A

M

V

N

V

V

A

G

P

P

E
|
E

V

L

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

-

N

-

P

-

D

-

P

-

D

-

R

-

P

-

L

-

T

-

A

-

P

-

V

L

G

D

A

A

N

N

G

G

R

N

A

P

E

T

T

T

V

-

Q

-

H

E

P

L

Y

T

D

D

M

Q

A

G

G

G

L

Y

E

F

E

D

F

F

E

A

P

P

V

L

-

D

-

R

-

A

-

Q

-

D

I

V

R

R

I

D

Y

I

D

D

Y

Y

A

A

A

L

A

E

-

H

A

M

G

G

L

F

P

Q

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

S

Q

Q

L

H

E
|
E

I

I

N
x
D

F
|
F

L

R

H
x
F

G

G

D

D

A

V

L

L

P

C

L

T

A

A

D

D

K

N

V

V

L

V

L

T

F

F

K

K

R

Y

L

V

T

V

R

K

Q

S

A

I

A

A

Q

Y

Q

H

S

K

G

G

M

Y

H

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

S

H
x
N

M

Q

S

S

V

L

I

F

D

K

E

D

A

-

G

G

R

K

T

N

L

V

F

F

A

Y

G

D

S

P

D

D

D

T

-

P

-

T

-

K

-

L

A

S

D

Q

T

D

A

A

M

M

F

Y

G

-

H

-

F

Y

I

I

G

G

L

L

Q

L

K

K

Y

H

V

I

P

R

E

E

I

F

M

T

P

A

L

V

F

T

A

N

P

P

N

V

V

V

N

N

S

S

F

Y

R

K

R
|
R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

V

N

Y

I

I

E

S

W

W

S

S

H

A

D

Q

N

N

R
|
R

S

S

C

S

G

L

L

I

R
|
R

V

I

P

P

A

A

G

T

G

R

R

G

A

N

A
x
G

R

T

R
|
R

V

I

E
|
E

N

L

R
|
R

L

C

P

P

G

D

A

P

D

A

S

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

A

G

L

S

M

L

L

L

R

A

A

G

G

Y

L

L

E

G

G

V

I

E

K

Q

N

K

K

L

I

A

D

R

P

S

G

P

E

E

P

A

T

S

N

G

V

N

N

A

I

Y

F

K

H

I

L

K

S

S

D

-

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

T

S

L

L

P

P

K

A

T

D

M

L

E

K

E

E

A

A

L

I

D

D

R

E

F

M

E

K

A

G

C

S

G

K

P

F

V

V

R

K

E

E

L

A

L

L

G

G

E

E

D

H

F

V

F

F

Q

S

T

H

Y

Y

L

L

R

C

V

A

K

K

S

E

V

M

E

E

L

W

D

D

L

E

F

Y

Q

K

G

A

V

V

T

H

S

P

W

W

E

E

R

L

D

S

H

R

L

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E149), D194 (≠ N223), F195 (= F224), F197 (≠ H226), N243 (≠ H272), R312 (= R340), R317 (= R345), G325 (≠ A353), R327 (= R355)
binding magnesium ion: E128 (= E149), E128 (= E149), E130 (= E151), E185 (= E214), E192 (= E221), E192 (= E221), H241 (= H270), E329 (= E357)
binding l-methionine-s-sulfoximine phosphate: E128 (= E149), E130 (= E151), E185 (= E214), E192 (= E221), G237 (= G266), H241 (= H270), R294 (= R322), E300 (≠ S328), R312 (= R340), R331 (= R359)

8ufjB Glutamine synthetase (see paper)
31% identity, 92% coverage: 34:456/459 of query aligns to 28:441/444 of 8ufjB

query
sites
8ufjB

D

D

M

T

N

L

G

G

V

I

L

I

R

K

G

S

K

W

A

A

L

I

P

P

T

A

A

E

K

Q

F

L

L

E

Q

E

A

A

L

F

E

E

D

N

R

G

A

V

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

Q

G

G

F

F

A

T

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

K

M

L

V

A

P

L

D

D

V

P

S

S

T

T

L

F

C

R

L

I

A

L

P

P

G

W

-

R

-

P

A

A

A

T

P

G

G

A

K

V

A

A

Y

R

V

I

F

L

A

G

D

D

A

V

F

Y

H

L

M

P

D

D

D

G

R

N

P

P

W

F

M

K

A

G

S

D

P

P

R

R

H

Y

V

V

L

L

R

K

A

T

V

A

L

I

D

K

L

E

Y

A

A

E

K

K

R

M

G

G

W

F

R

S

A

M

V

N

V

V

A

G

P

P

E
|
E

V

L

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

-

N

-

P

-

D

-

P

-

D

-

R

-

P

-

L

-

T

-

A

-

P

-

V

L

G

D

A

A

N

N

G

G

R

N

A

P

E

T

T

T

V

-

Q

-

H

E

P

L

Y

T

D

D

M

Q

A

G

G

G

L

Y

E

F

E

D

F

F

E

A

P

P

V

L

-

D

-

L

-

G

-

Q

-

D

I

V

R

R

I

D

Y

I

D

D

Y

Y

A

A

A

L

A

E

-

H

A

M

G

G

L

F

P

Q

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

S

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

R

H

F

G

G

D

D

A

V

L

L

P

C

L

T

A

A

D

D

K

N

V

V

L

V

L

T

F

F

K

K

R

Y

L

V

T

V

R

K

Q

S

A

I

A

A

Q

Y

Q

H

S

K

G

G

M

Y

H

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G

G

S

S

S

G

M

M

H
|
H

L

S

H

N

M

Q

S

S

V

L

I

F

D

K

E

D

A

-

G

G

R

K

T

N

L

V

F

F

A

Y

G

D

S

P

D

D

D

T

-

P

-

T

-

K

-

L

A

S

D

Q

T

D

A

A

M

M

F

Y

G

-

H

-

F

Y

I

I

G

G

L

L

Q

L

K

K

Y

H

V

I

P

R

E

E

I

F

M

T

P

A

L

V

F

T

A

N

P

P

N

V

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N

G

H

Y

S

E

A

A

P

P

A

V

N

Y

I

I

E

S

W

W

S

S

H

A

D

Q

N

N

R

R

S

S

C

S

G

L

L

I

R

R

V

I

P

P

A

A

G

T

G

R

R

G

A

N

A

G

R

T

R

R

V

I

E
|
E

N

L

R

R

L

C

P

P

G

D

A

P

D

A

S

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

A

G

L

S

M

L

L

L

R

A

A

G

G

Y

L

L

E

G

G

V

I

E

K

Q

N

K

K

L

I

A

D

R

P

S

G

P

E

E

P

A

T

S

N

G

V

N

N

A

I

Y

F

K

H

I

L

K

S

S

D

-

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

T

S

L

L

P

P

K

A

T

D

M

L

E

K

E

E

A

A

L

I

D

D

R

E

F

M

E

K

A

G

C

S

G

K

P

F

V

V

R

K

E

E

L

A

L

L

G

G

E

E

D

H

F

V

F

F

Q

S

T

H

Y

Y

L

L

R

C

V

A

K

K

S

E

V

M

E

E

L

W

D

D

L

E

F

Y

Q

K

G

A

V

V

T

H

S

P

W

W

E

E

R

L

D

S

H

R

L

Y

L

L

binding magnesium ion: E132 (= E149), E134 (= E151), E189 (= E214), E196 (= E221), H245 (= H270), E333 (= E357)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
31% identity, 91% coverage: 34:451/459 of query aligns to 23:439/446 of 8ooqB

query
sites
8ooqB

D

D

M

I

N

L

G

G

V

V

L

P

R

K

G

N

K

V

A
|
A

L

F

P

P

T

I

A

K

K

A

F

G

L

E

Q

K

A

G

L

I

E

E

D

E

R

L

A

R

L

D

Y

V

L

L

P

E

S

N

S

G

A

L

F

Y

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

F

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

V

-

G

Y

I

S

N

D

E

P

S

D

D

M

M

R

M

M

L

V

K

P

P

D

D

V

L

S

S

T

T

L

F

C

S

L

V

A

L

P

P

G

W

A
x
R

A

P

P

S

G

E

K

K

-

S

-
x
V

A

A

Y

R

V

V

F

I

A

C

D

D

A

V

F

Y

H

T

M

T

D

K

D

G

R

K

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

G

V

C

L

L

R

K

A

R

V

V

L

M

D

E

L

E

Y

F

A

K

K

K

R

E

-

F

G

N

W

G

R

E

A

Y

V

F

V

V

A

G

P

P

E

E

V

P

E
|
E

F

F

Y

F

L

L

T

L

A

K

P

K

N

D

P

P

-

H

D

N

P

P

D

H

R

K

P

Y

L

I

T

P

A

A

P

D

V

D

G

G

A

G

N

Y

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

E

G

P

L

M

E

D

E

E

F

-

E

A

P
|
P

V

D

I

I

R
|
R

I

D

Y

I

D

V

Y

F

A

A

A

L

A

E

A

N

-

L

G

G

L

F

P

H

L

V

D

E

T

A

L
x
S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

V

N

D

F

F

L

K

H

F

G

D

D

D

A

A

L

L

P

K

L

T

A

A

D

D

K

S

V

V

L

I

L

T

F

F

K

K

R

T

L

T

T

I

R

K

Q

T

A

I

A

A

Q

E

Q

Q

S

H

G

G

M

L

H

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

A

F

A

G

Q

M

A

N

G

G

S

S

S

G

M

M

H

H

L

C

H

H

M

Q

S

S

V

I

I

W

D

L

E

N

A

G

G

E

R

P

T

S

L

F

F

Y

-

D

-

E

-

N

A

A

G

P

S

Y

D

Q

D

L

A

S

D

E

T

T

A

C

M

M

F

-

G

-

H

N

F

Y

I

V

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

K

E

A

I

I

M

V

P

A

L

I

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N

G

H

Y

S

E

A

A

P

P

A

V

N

N

I

I

E

A

W

W

S

A

H

N

D

S

N

N

R

R

S

S

C

A

G

I

L

I

R

R

V

V

P

P

A

A

G

A

G

R

R

G

A

K

A

G

R

T

R

R

V

I

E

E

N

F

R

R

L

A

P

P

G

D

A

P

D

S

S

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

T

G

V

S

M

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

V

E

K

Q

N

K

K

L

L

A

D

R

A

S

P

P

E

E

P

A

V

S

E

G

R

N

N

A

I

Y

F

K

A

I

M

K

S

-

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

S

E

T

S

L

V

P

P

K

A

T

N

M

L

E

K

E

A

A

A

L

L

D

D

R

E

F

L

E

E

A

N

C

N

G

D

P

V

V

L

R

K

E

N

L

A

L

L

G

G

E

K

D

H

F

I

F

F

Q

E

T

S

Y

F

L

L

R

E

V

I

K

K

S

N

V

A

E

E

L

W

D

D

L

S

F

F

Q

R

G

T

V

S

V

V

T

T

S

D

W

W

E

E

binding 2-oxoglutaric acid: A32 (= A43), R86 (≠ A103), V92 (vs. gap), P169 (= P192), R172 (= R195), R173 (= R196), S189 (≠ L211)
binding magnesium ion: E137 (= E151), E192 (= E214), E199 (= E221)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
31% identity, 91% coverage: 34:451/459 of query aligns to 24:440/447 of 8oooA

query
sites
8oooA

D

D

M

I

N

L

G

G

V

V

L

P

R

K

G

N

K

V

A
|
A

L

F

P

P

T

I

A

K

K

A

F

G

L

E

Q

K

A

G

L

I

E

E

D

E

R

L

A

R

L

D

Y

V

L

L

P

E

S

N

S

G

A

L

F

Y

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

F

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

-

V

A

G

Y

I

S

N

D

E

P

S

D

D

M

M

R

M

M

L

V

K

P

P

D

D

V

L

S

S

T

T

L

F

C

S

L

V

A

L

P

P

G

W

A
x
R

A

P

P

S

G

E

K

K

-

S

-
x
V

A

A

Y

R

V

V

F

I

A

C

D

D

A

V

F

Y

H

T

M

T

D

K

D

G

R

K

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

G

V

C

L

L

R

K

A

R

V

V

L

M

D

E

L

E

Y

F

A

K

K

K

R

E

-

F

G

N

W

G

R

E

A

Y

V

F

V

V

A

G

P

P

E
|
E

V

P

E

E

F

F

Y

F

L

L

T

L

A

K

P

K

N

D

P

P

-

H

D

N

P

P

D

H

R

K

P

Y

L

I

T

P

A

A

P

D

V

D

G

G

A

G

N

Y

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

E

G

P

L

M

E

D

E

E

F

-

E

A

P
|
P

V

D

I

I

R
|
R

I

D

Y

I

D

V

Y

F

A

A

A

L

A

E

A

N

-

L

G

G

L

F

P

H

L

V

D
x
E

T

A

L
x
S

I

H

H

H

E

E

S

V

G

A

T

P

A

G

Q

Q

L

H

E

E

I

V

N

D

F
|
F

L
x
K

H
x
F

G

D

D

D

A

A

L

L

P

K

L

T

A

A

D

D

K

S

V

V

L

I

L

T

F

F

K

K

R

T

L

T

T

I

R

K

Q

T

A

I

A

A

Q

E

Q

Q

S

H

G

G

M

L

H

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

A

F

A

G

Q

M

A

N

G

G

S

S

S

G

M

M

H

H

L

C

H
|
H

M

Q

S
|
S

V

I

I

W

D

L

E

N

A

G

G

E

R

P

T

S

L

F

F

Y

-

D

-

E

-

N

A

A

G

P

S

Y

D

Q

D

L

A

S

D

E

T

T

A

C

M

M

F

-

G

-

H

N

F

Y

I

V

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

K

E

A

I

I

M

V

P

A

L

I

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N

G

H

Y

S

E

A

A

P

P

A

V

N

N

I

I

E

A

W

W

S

A

H

N

D

S

N

N

R

R

S

S

C

A

G

I

L

I

R
|
R

V

V

P

P

A

A

G

A

G

R

R

G

A

K

A

G

R

T

R
|
R

V

I

E

E

N

F

R

R

L

A

P

P

G

D

A

P

D

S

S

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

T

G

V

S

M

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

V

E

K

Q

N

K

K

L

L

A

D

R

A

S

P

P

E

E

P

A

V

S

E

G

R

N

N

A

I

Y

F

K

A

I

M

K

S

-

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

S

E

T

S

L

V

P

P

K

A

T

N

M

L

E

K

E

A

A

A

L

L

D

D

R

E

F

L

E

E

A

N

C

N

G

D

P

V

V

L

R

K

E

N

L

A

L

L

G

G

E

K

D

H

F

I

F

F

Q

E

T

S

Y

F

L

L

R

E

V

I

K

K

S

N

V

A

E

E

L

W

D

D

L

S

F

F

Q

R

G

T

V

S

V

V

T

T

S

D

W

W

E

E

binding 2-oxoglutaric acid: A33 (= A43), R87 (≠ A103), V93 (vs. gap), P170 (= P192), R173 (= R195), R174 (= R196), S190 (≠ L211)
binding adenosine-5'-triphosphate: E136 (= E149), E188 (≠ D209), F203 (= F224), K204 (≠ L225), F205 (≠ H226), H251 (= H272), S253 (= S274), R325 (= R345), R335 (= R355)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
30% identity, 92% coverage: 34:456/459 of query aligns to 26:440/443 of 7tf9S

query
sites
7tf9S

D

D

M

I

N

L

G

G

V

I

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

K

Q

K

A

A

L

L

E

D

D

N

R

K

A

I

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F
|
F

A
x
V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

F

P

P

D

D

V

L

S

D

T

T

L

W

C

V

L

V

A

F

P

P

G

W

A

T

A

A

-

E

P

K

G

G

K

K

-

V

A

A

Y

R

V

M

F

I

A

C

D

D

A

I

F

Y

H

N

M

P

D

D

D

M

R

T

P

P

W

F

M

A

A

G

S

D

P

P

R

R

H

A

V

N

L

L

R

K

A

R

V

V

L

L

D

K

L

E

Y

M

A

E

K

E

R

L

G

G

W

F

R

T

A

E

V

F

-

N

V

L

A

G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

P

-

N

K

P

L

D

D

P

E

D

N

R

R

P

R

L

P

T

T

A

L

P

E

V

L

G

N

A

D

N

S

G

G

R

G

A
x
Y

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

E

D

D

A

A

L

I

P

T

L

A

A

C

D

D

K

S

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G
|
G

S

S

S
x
G

M

M

H
|
H

L

F

H

N

M

M

S

S

V

L

I

F

D

N

E

E

A

K

G

G

R

N

T

A

L

F

F

F

A

D

G

E

S

S

D

G

D

E

A

L

D

E

T

L

A

S

M

Q

F

T

G

A

-

Y

H

H

F

F

I

L

G

A

G

G

L

M

Q

L

K

K

Y

H

V

A

P

R

E

G

I

Y

M

T

P

A

L

V

F

T

A

N

P

P

N

T

V

I

N

N

S

S

F

F

R

K

R
|
R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

C

N

Y

I

I

E

A

W

W

S

S

H

G

D

K

N

N

R

R

S

S

C

P

G

L

L

V

R

R

V

V

P

P

A

S

G

S

G

R

R

G

A

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

L

S

P

V

G

D

A

P

D

S

S

A

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

G

V

S

L

L

L

L

K

A

A

G

G

Y

L

L

S

G

G

V

I

E

K

Q

D

K

E

L

L

A

T

R

P

S

P

P

A

E

P

A

V

S

D

G

R

N

N

A

I

Y

Y

K

G

I

M

K

N

S

E

T

E

-

E

-

R

-

E

-

A

-

T

-

G

-

I

-

Y

-

D

L

L

P

P

K

E

T

S

M

L

E

G

E

H

A

A

L

L

D

I

R

E

F

L

E

E

A

K

C

N

G

E

P

I

V

I

R

K

E

D

L

G

L

L

G

G

E

E

D

H

F

I

F

F

Q

E

T

H

Y

F

L

I

R
x
E

V

A

K

K

S

T

V
x
I

E

E

L

C

D

D

L
x
M

F

F

Q

R

G

T

V

A

V

V

T

H

S

P

W

W

E

E

R

R

D

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E151), Y155 (≠ A175), E188 (= E214), G240 (= G266), G242 (≠ S268), R297 (= R322), E303 (≠ S328)
binding magnesium ion: E131 (= E149), E133 (= E151), E188 (= E214), E195 (= E221), H244 (= H270), E332 (= E357)
binding : F59 (= F81), V60 (≠ A82), E418 (≠ R434), I422 (≠ V438), M426 (≠ L442)

7tenA Glutamine synthetase (see paper)
30% identity, 92% coverage: 34:456/459 of query aligns to 25:439/442 of 7tenA

query
sites
7tenA

D

D

M

I

N

L

G

G

V

I

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

K

Q

K

A

A

L

L

E

D

D

N

R

K

A

I

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

F

P

P

D

D

V

L

S

D

T

T

L

W

C

V

L

V

A

F

P

P

G

W

A

T

A

A

-

E

P

K

G

G

K

K

-

V

A

A

Y

R

V

M

F

I

A

C

D

D

A

I

F

Y

H

N

M

P

D

D

D

M

R

T

P

P

W

F

M

A

A

G

S

D

P

P

R

R

H

A

V

N

L

L

R

K

A

R

V

V

L

L

D

K

L

E

Y

M

A

E

K

E

R

L

G

G

W

F

R

T

A

E

V

F

-

N

V

L

A
x
G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

P

-

N

K

P

L

D

D

P

E

D

N

R

R

P

R

L

P

T

T

A

L

P

E

V

L

G

N

A

D

N

S

G

G

R

G

A

Y

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F
|
F

L
x
K

H
x
Y

G

E

D

D

A

A

L

I

P

T

L

A

A

C

D

D

K

S

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A
x
N

G
|
G

S

S

S

G

M

M

H
|
H

L

F

H
x
N

M

M

S
|
S

V

L

I

F

D

N

E

E

A

K

G

G

R

N

T

A

L

F

F

F

A

D

G

E

S

S

D

G

D

E

A

L

D

E

T

L

A

S

M

Q

F

T

G

A

-

Y

H

H

F

F

I

L

G

A

G

G

L

M

Q

L

K

K

Y

H

V

A

P

R

E

G

I

Y

M

T

P

A

L

V

F

T

A

N

P

P

N

T

V

I

N

N

S

S

F

F

R

K

R
|
R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

C

N

Y

I

I

E

A

W

W

S

S

H

G

D

K

N

N

R
|
R

S

S

C

P

G

L

L

V

R
|
R

V

V

P

P

A

S

G

S

G

R

R

G

A

L

A
x
S

R

T

R
|
R

V

L

E

E

N

L

R
|
R

L

S

P

V

G

D

A

P

D

S

S

A

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

G

V

S

L

L

L

L

K

A

A

G

G

Y

L

L

S

G

G

V

I

E

K

Q

D

K

E

L

L

A

T

R

P

S

P

P

A

E

P

A

V

S

D

G

R

N

N

A

I

Y

Y

K

G

I

M

K

N

S

E

T

E

-

E

-

R

-

E

-

A

-

T

-

G

-

I

-

Y

-

D

L

L

P

P

K

E

T

S

M

L

E

G

E

H

A

A

L

L

D

I

R

E

F

L

E

E

A

K

C

N

G

E

P

I

V

I

R

K

E

D

L

G

L

L

G

G

E

E

D

H

F

I

F

F

Q

E

T

H

Y

F

L

I

R

E

V

A

K

K

S

T

V

I

E

E

L

C

D

D

L

M

F

F

Q

R

G

T

V

A

V

V

T

H

S

P

W

W

E

E

R

R

D

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A147), E130 (= E149), E182 (≠ D209), D196 (≠ N223), F197 (= F224), K198 (≠ L225), Y199 (≠ H226), N245 (≠ H272), S247 (= S274), R319 (= R345), S327 (≠ A353), R329 (= R355)
binding l-methionine-s-sulfoximine phosphate: E130 (= E149), E132 (= E151), E187 (= E214), E194 (= E221), N238 (≠ A265), G239 (= G266), H243 (= H270), R296 (= R322), E302 (≠ S328), R314 (= R340), R333 (= R359)

8ooxB Glutamine synthetase (see paper)
31% identity, 92% coverage: 34:456/459 of query aligns to 24:435/438 of 8ooxB

query
sites
8ooxB

D

D

M

V

N

Q

G

G

V

I

L

P

R

K

G

N

K

V

A

A

L

I

P

P

T

V

A

G

K

Q

F

L

L

E

Q

K

A

A

L

L

E

G

D

-

R

-

A

-

L

-

Y

-

L

-

P

P

S

G

S

I

A

H

F

F

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

V

M

L

V

R

P

P

D

D

V

P

S

D

T

T

L

F

C

R

L

V

A

L

P

P

G

W

A

S

A

G

-

N

P

T

G

A

K

E

A

A

Y

R

V

L

F

I

A

C

D

D

A

I

F

E

H

L

M

P

D

D

D

G

R

K

P

P

W

F

M

M

A

G

S

C

P

P

R

R

H

Q

V

V

L

L

R

K

A

K

V

N

L

M

D

E

L

E

Y

A

A

A

K

K

R

L

G

G

W

Y

R

V

A

M

V

N

V

T

A

G

P

P

E

E

V

M

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

R

N

Q

P

D

D

G

P

-

D

-

R

M

P

P

L

T

T

N

A

I

P

P

V

Q

G

D

A

R

N

G

G

G

R

Y

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

I

E

D

E

L

F

A

E

E

P

E

V

I

I

K

R

R

R

E

I

I

Y

V

D

L

Y

V

A

L

A

E

A

M

G

G

L

F

P

E

L

V

D

E

T

A

L

A

I

H

H

H

E
|
E

S

V

G

A

T

F

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

D

D

N

A

A

L

L

P

A

L

T

A

A

D

D

K

N

V

V

L

I

L

T

F

L

K

K

R

Y

L

V

T

A

R

K

Q

T

A

L

A

A

Q

L

Q

Q

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

I

A

F

A

G

Q

V

A

N

G

G

S

S

S

G

M

M

H

H

L

T

H

N

M

T

S

S

V

L

I

F

D

K

E

D

A

-

G

G

R

K

T

N

L

A

F

F

A

Y

G

D

S

P

D

D

-

A

-

P

D

D

A

Q

D

I

T

S

A

D

M

T

F

L

G

R

H

Y

F

F

I

V

G

G

L

V

Q

L

K

K

Y

H

V

I

P

R

E

A

I

I

M

T

P

A

L

I

F

T

A

N

P

P

N

L

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N

G

H

Y

S

E

A

A

P

P

A

V

N

Y

I

I

E

T

W

W

S

S

H

G

D

P

N

N

R

R

S

S

C

S

G

L

L

I

R

R

V

V

P

P

A

A

G

P

G

R

R

G

A

N

A

S

R

T

R

R

V

I

E

E

N

I

R

R

L

S

P

P

G

D

A

P

D

S

S

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

A

G

A

S

I

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

V

E

K

Q

N

K

K

L

I

A

E

R

P

S

P

P

E

E

R

A

V

S

E

G

K

N

N

A

I

Y

Y

K

K

I

L

K

T

S

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

T

M

L

L

P

P

K

G

T

T

M

L

E

K

E

E

A

A

L

I

D

E

R

C

F

F

E

K

A

E

C

D

G

E

P

L

V

L

R

V

E

S

L

A

L

L

G

G

E

E

D

H

F

V

F

S

Q

Q

T

S

Y

I

L

I

R

N

V

V

K

A

S

M

V

A

E

D

L

W

D

D

L

S

F

Y

Q

R

G

T

V

Q

V

V

T

H

S

Q

W

W

E

E

R

L

D

D

H

R

L

Y

L

L

binding magnesium ion: E129 (= E151), E183 (= E214), E190 (= E221)

7tfaB Glutamine synthetase (see paper)
28% identity, 92% coverage: 34:456/459 of query aligns to 25:438/441 of 7tfaB

query
sites
7tfaB

D

D

M

L

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

E

Q

K

A

A

L

L

E

D

D

N

R

K

A

M

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F
x
Y

A

V

-
x
R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

Y

P

P

D

D

V

L

S

D

T

T

L

W

C

V

L

V

A

F

P

P

G

W

A

V

A

T

P

S

G

D

K

R

-

V

A

A

Y

R

V

L

F

I

A

C

D

D

A

I

F

Y

H

K

M

P

D

D

D

G

R

S

P

P

W

F

M

A

A

G

S

D

P

P

R

R

H

G

V

I

L

L

R

K

A

R

V

V

L

L

D

K

L

E

Y

A

A

E

K

E

R

L

G

G

W

Y

R

T

A

S

V

M

-

N

V

V

A

G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

T

T

A

D

P

E

N

K

P

G

D

D

P

P

D

T

R

T

P

E

L

L

T

N

A

D

P

Q

V

G

G

G

A
x
Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

M

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

E

I

I

Y

V

D

L

Y

K

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S
x
V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

D

A

A

L

V

P

K

L

A

A

A

D

D

K

Q

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

Q

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A
x
N

G
|
G

S

S

S

G

M

M

H
|
H

L

C

H

N

M

Q

S

S

V

L

I

F

D

K

E

D

A

N

G

E

R

N

T

V

L

F

F

Y

A

D

G

E

S

T

D

D

D

E

A

L

D

G

T

L

A

S

M

Q

F

T

G

A

-

R

H

H

F

Y

I

M

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

R

E

A

I

M

M

A

P

A

L

I

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

I

L

R

V

P

P

N
x
G

H
x
Y

S
x
E

A

A

P

P

A

C

N

Y

I

V

E

A

W

W

S

S

H

A

D

S

N

N

R
|
R

S

S

C

P

G

M

L

I

R

R

V

I

P

P

A

A

G

S

G

R

R

G

A

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

L

N

P

P

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

A

G

V

S

M

L

L

L

R

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

R

K

Q

L

M

A

A

R

L

S

P

P

A

E

P

A

I

S

D

G

R

N

N

A

I

Y

Y

K

V

I

M

K

S

S

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

T

S

L

L

P

P

K

A

T

D

M

L

E

K

E

E

A

A

L

L

D

S

R

E

F

L

E

I

A

R

C

S

G

E

P

V

V

I

R

S

E

D

L

A

L

L

G

G

E

D

D

H

F

A

F

L

Q

A

T

Y

Y

F

L

Y

R

E

V

L

K

K

S

E

V

I

E

E

L

W

D

D

L
x
M

F

Y

Q

R

G

T

V

Q

V

V

T

H

S

Q

W

W

E

E

R

R

D

D

H

Q

L

Y

L

L

binding glutamine: E131 (= E151), Y153 (≠ A171), E186 (= E214), G238 (= G266), H242 (= H270), R295 (= R322), E301 (≠ S328)
binding magnesium ion: E129 (= E149), E131 (= E151), E186 (= E214), E193 (= E221), H242 (= H270), E330 (= E357)
binding : Y58 (≠ F81), R60 (vs. gap), V187 (≠ S215), N237 (≠ A265), G299 (≠ N326), Y300 (≠ H327), R313 (= R340), M424 (≠ L442)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
28% identity, 92% coverage: 34:456/459 of query aligns to 25:436/439 of 7tdpA

query
sites
7tdpA

D

D

M

L

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

E

Q

K

A

A

L

L

E

D

D

N

R

K

A

M

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

Y

A

V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

Y

P

P

D

D

V

L

S

D

T

T

L

W

C

V

L

V

A

F

P

P

G

W

A

S

A

-

P

D

G

R

K

V

A

A

Y

R

V

L

F

I

A

C

D

D

A

I

F

Y

H

K

M

P

D

D

D

G

R

S

P

P

W

F

M

A

A

G

S

D

P

P

R

R

H

G

V

I

L

L

R

K

A

R

V

V

L

L

D

K

L

E

Y

A

A

E

K

E

R

L

G

G

W

Y

R

T

A

S

V

M

-
x
N

V

V

A
x
G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

T

T

A

D

P

E

N

K

P

G

D

D

P

P

D

T

R

T

P

E

L

L

T

N

A

D

P

Q

V

G

G

G

A

Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

M

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

E

I

I

Y

V

D

L

Y

K

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F

F

L

K

H
x
Y

G

A

D

D

A

A

L

V

P

K

L

A

A

A

D

D

K

Q

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

Q

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

C

H
x
N

M

Q

S
|
S

V

L

I

F

D

K

E

D

A

N

G

E

R

N

T

V

L

F

F

Y

A

D

G

E

S

T

D

D

D

E

A

L

D

G

T

L

A

S

M

Q

F

T

G

A

-

R

H

H

F

Y

I

M

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

R

E

A

I

M

M

A

P

A

L

I

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

C

N

Y

I

V

E

A

W

W

S

S

H

A

D

S

N

N

R
|
R

S

S

C

P

G

M

L

I

R
|
R

V

I

P

P

A

A

G

S

G

R

R

G

A

L

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

N

P

P

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

A

G

V

S

M

L

L

L

R

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

R

K

Q

L

M

A

A

R

L

S

P

P

A

E

P

A

I

S

D

G

R

N

N

A

I

Y

Y

K

V

I

M

K

S

S

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

T

S

L

L

P

P

K

A

T

D

M

L

E

K

E

E

A

A

L

L

D

S

R

E

F

L

E

I

A

R

C

S

G

E

P

V

V

I

R

S

E

D

L

A

L

L

G

G

E

D

D

H

F

A

F

L

Q

A

T

Y

Y

F

L

Y

R

E

V

L

K

K

S

E

V

I

E

E

L

W

D

D

L

M

F

Y

Q

R

G

T

V

Q

V

V

T

H

S

Q

W

W

E

E

R

R

D

D

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A147), E127 (= E149), E179 (≠ D209), D193 (≠ N223), Y196 (≠ H226), N242 (≠ H272), S244 (= S274), R316 (= R345), R326 (= R355)
binding magnesium ion: E127 (= E149), E127 (= E149), E129 (= E151), E184 (= E214), E191 (= E221), E191 (= E221), H240 (= H270), E328 (= E357)
binding l-methionine-s-sulfoximine phosphate: E127 (= E149), E129 (= E151), E184 (= E214), E191 (= E221), G236 (= G266), H240 (= H270), R293 (= R322), E299 (≠ S328), R311 (= R340), R330 (= R359)

8oozA Glutamine synthetase (see paper)
31% identity, 92% coverage: 34:456/459 of query aligns to 24:427/430 of 8oozA

query
sites
8oozA

D

D

M

V

N

Q

G

G

V

I

L

P

R

K

G

N

K

V

A

A

L

I

P

P

T

V

A

G

K

Q

F

L

L

E

Q

K

A

A

L

L

E

G

D

-

R

-

A

-

L

-

Y

-

L

-

P

P

S

G

S

I

A

H

F

F

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

-

A

-

Y

-

S

-

D

-

P

S

D

D

M

M

R

V

M

L

V

R

P

P

D

D

V

P

S

D

T

T

L

F

C

R

L

V

A

L

P

P

G

W

A

S

A

T

P

-

G

A

K

E

A

A

Y

R

V

L

F

I

A

C

D

D

A

I

F

E

H

L

M

P

D

D

D

G

R

K

P

P

W

F

M

M

A

G

S

C

P

P

R

R

H

Q

V

V

L

L

R

K

A

K

V

N

L

M

D

E

L

E

Y

A

A

A

K

K

R

L

G

G

W

Y

R

V

A

M

V

N

V

T

A
x
G

P

P

E
|
E

V

M

E

E

F

F

Y

F

L

L

T

F

A

K

P

R

N

Q

P

D

D

G

P

-

D

-

R

M

P

P

L

T

T

N

A

I

P

P

V

Q

G

D

A

R

N

G

G

G

R

Y

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

I

E

D

E

L

F

A

E

E

P

E

V

I

I

K

R

R

R

E

I

I

Y

V

D

L

Y

V

A

L

A

E

A

M

G

G

L

F

P

E

L

V

D
x
E

T

A

L

A

I

H

H

H

E

E

S

V

G

A

T

F

A

G

Q

Q

L

H

E

E

I

I

N

D

F
|
F

L
x
K

H
x
Y

G

D

D

N

A

A

L

L

P

A

L

T

A

A

D

D

K

N

V

V

L

I

L

T

F

L

K

K

R

Y

L

V

T

A

R

K

Q

T

A

L

A

A

Q

L

Q

Q

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

I

A

F

A

G

Q

V

A

N

G

G

S

S

S

G

M

M

H
|
H

L

T

H
x
N

M

T

S
|
S

V

L

I

F

D

K

E

D

A

-

G

G

R

K

T

N

L

A

F

F

A

Y

G

D

S

P

D

D

-

A

-

P

D

D

A

Q

D

I

T

S

A

D

M

T

F

L

G

R

H

Y

F

F

I

V

G

G

L

V

Q

L

K

K

Y

H

V

I

P

R

E

A

I

I

M

T

P

A

L

I

F

T

A

N

P

P

N

L

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N

G

H

Y

S

E

A

A

P

P

A

V

N

Y

I

I

E

T

W

W

S

S

H

G

D

P

N

N

R

R

S

S

C

S

G

L

L

I

R

R

V

V

P

P

A

A

G

P

G

R

R

G

A

N

A
x
S

R

T

R
|
R

V

I

E
|
E

N

I

R

R

L

S

P

P

G

D

A

P

D

S

S

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

A

G

A

S

I

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

V

E

K

Q

N

K

K

L

I

A

E

R

P

S

P

P

E

E

R

A

V

S

E

G

K

N

N

A

I

Y

Y

K

K

I

L

K

T

S

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

T

M

L

L

P

P

K

G

T

T

M

L

E

K

E

E

A

A

L

I

D

E

R

C

F

F

E

K

A

E

C

D

G

E

P

L

V

L

R

V

E

S

L

A

L

L

G

G

E

E

D

H

F

V

F

S

Q

Q

T

S

Y

I

L

I

R

N

V

V

K

A

S

M

V

A

E

D

L

W

D

D

L

S

F

Y

Q

R

G

T

V

Q

V

V

T

H

S

Q

W

W

E

E

R

L

D

D

H

R

L

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ A147), E170 (≠ D209), F185 (= F224), K186 (≠ L225), Y187 (≠ H226), N233 (≠ H272), S235 (= S274), S315 (≠ A353), R317 (= R355)
binding magnesium ion: E119 (= E149), H231 (= H270), E319 (= E357)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
35% identity, 79% coverage: 90:451/459 of query aligns to 56:390/396 of 5dm3C

query
sites
5dm3C

M

M

V

K

P

P

D

D

V

L

S

A

T

T

L

L

C

R

L

C

A

V

P

P

G

W

A

L

A

-

P

E

G

G

K

T

A

A

Y

M

V

V

F

L

A

C

D

D

A

L

F

L

-

D

H

H

M

R

D

T

D

H

R

A

P

E

W

V

M

P

A

H

S

A

P

P

R

R

H

A

V

I

L

L

R

K

A

R

V

Q

L

L

D

A

L

R

Y

L

A

E

K

A

R

M

G

G

W

L

R

E

A

A

V

I

V

M

A

A

P

T

E
|
E

V

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

P

K

N

S

P

L

D

D

P

E

D

I

R

R

P

-

L

-

T

-

A

-

P

-

V

-

G

-

A

-

N

K

G

G

R

R

A

F

E

R

T

T

V

T

Q

K

H

E

P

-

Y

-

D

-

M

-

A

-

G

-

L

-

E

-

F

-

E

E

P

H

V

V

I

L

R

R

R

P

I

L

Y

R

D

N

Y

H

A

L

A

H

A

A

G

G

L

I

P

P

L

V

D

E

T

G

L

T

I

K

H

G

E
|
E

S

A

G

G

T

A

A

G

Q

Q

L

E

E
|
E

I

L

N

N

F

I

L
x
R

H
x
C

G

A

D

K

A

A

L

L

P

D

L

T

A

A

D

D

K

Y

V

H

L

T

L

I

F

A

K

K

R

H

L

A

T

T

R

K

Q

E

A

I

A

A

Q

W

Q

Q

S

Q

G

G

M

R

H

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

A

H

A

A

Q

H

A

A

G

G

S

S

M

S

H
|
H

L

I

H
|
H

M

Q

S
|
S

V

L

I

W

D

K

E

Q

A

-

G

G

R

L

T

P

L

A

F

F

A

H

G

D

S

E

D

R

D

D

A

A

-

L

-

G

D

M

T

S

A

A

M

L

F

M

G

K

H

H

F

Y

I

L

G

A

G

G

L

L

Q

L

K

K

Y

Y

V

A

P

P

E

D

I

Y

M

T

P

Y

L

F

F

L

A

A

P

P

N

Y

V

L

N

N

S

S

F

Y

R

K

R

R

I

F

R

Q

P

-

N

-

H

-

S

F

A

A

P

P

A

T

N

R

I

T

E

V

W

W

S

S

H

V

D

D

N

N

R
|
R

S

T

C

A

G

G

L

F

R

R

V

L

P

C

A

A

G

E

G

G

R

T

A

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

L

I

P

G

G

G

A

S

D

D

S

L

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

G

G

S

Q

L

L

L

A

A

A

G

G

Y

I

L

K

G

G

V

I

E

E

Q

E

K

C

L

L

A

A

R

L

S

P

P

P

E

P

A

A

S

S

G

G

N

-

A

-

Y

-

K

-

I

-

K

-

S

-

T

L

L

I

P

P

K

Q

T

N

M

L

E

R

E

D

A

A

L

M

D

E

R

A

F

L

E

R

A

G

C

S

G

T

P

M

V

L

R

R

E

E

L

A

L

M

G

G

E

E

D

D

F

V

Q

D

T

H

Y

Y

L

V

R

R

V

A

K

A

S

E

V

V

E

E

L

L

D

E

L

D

F

F

Q

Q

G

R

V

V

T

S

S

D

W

Y

E

E

active site: E115 (= E149), E117 (= E151), E162 (= E214), E169 (= E221), H218 (= H270), R286 (= R340), E303 (= E357), R305 (= R359)
binding adenosine-5'-diphosphate: R173 (≠ L225), C174 (≠ H226), H220 (= H272), S222 (= S274), R301 (= R355)

4s0rD Structure of gs-tnra complex (see paper)
28% identity, 96% coverage: 15:456/459 of query aligns to 11:444/447 of 4s0rD

query
sites
4s0rD

D

D

A

I

K

E

A

K

W

L

L

V

T

K

R

E

H

E

G

N

I

V

N

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

G

Q

K

A

A

L

L

E

D

D

N

R

K

A

V

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S
x
D

G

G

S

S

I

S

D

I

E

E

G

G

F
|
F

A
x
V

-
x
R

Y
x
I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

Y

P

P

D

D

V

L

S

N

T

T

L

F

C

V

L

I

A

F

P

P

G

W

A

T

A

A

-

E

P

K

G

G

K

K

A

V

Y

A

V

R

F

F

-

I

A

C

D

D

A

I

F

Y

H

N

M

P

D

D

D

G

R

T

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

N

V

N

L

L

R

K

A

R

V

I

L

L

D

K

L

E

Y

M

A

E

K

D

R

L

G

G

W

F

R

S

A

D

V

F

-

N

V

L

A

G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

L

N

D

P

E

-

K

-

G

D

E

P

P

D

T

R

L

P

E

L

L

T

N

A

D

P

K

V

G

G

G

A

Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D
|
D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

G

A

A

L

V

P

R

L

S

A

C

D

D

K

D

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A
x
G

Q
x
V

A
x
N

G

G

S

S

S

G

M

M

H
|
H

L

C

H

N

M

L

S

S

V

L

I

F

D

K

E

N

A

G

G

V

R

N

T

A

L

F

F

F

A

D

G

E

S

N

D

A

D

D

-

L

-

Q

-

L

A

S

D

E

T

T

A

A

M

-

F

-

G

K

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

E

S

I

F

M

T

P

A

L

V

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

I

L

R

V

P

P

N
x
G

H
x
Y

S
x
E

A

A

P

P

A

C

N

Y

I

V

E

A

W

W

S

S

H

A

D

Q

N

N

R
|
R

S

S

C

P

G

L

L

I

R

R

V

I

P

P

A

A

G

S

G

R

R

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

G

V

S

L

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

N

K

K

L

L

A

E

R

A

S

P

P

A

E

P

A

I

S

D

G

R

N

N

A

I

Y
|
Y

K

V

I

M

K

S

S

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

K

A

T

T

M

L

E

A

E

E

A

A

L

L

D

E

R

E

F

F

E

K

A

S

C

N

G

E

P

V

V

M

R

V

E

K

L

A

L

L

G

G

E

E

D

H

F

L

F

F

Q

E

T
x
H

Y

F

L

I

R

E

V

A

K

K

S

E

V
x
I

E

E

L

W

D

D

L
x
M

F

F

Q

R

G

T

V

Q

V

V

T

H

S

P

W

W

E

E

R

R

D

E

H

Q

L

Y

L

M

active site: D56 (≠ S74), E135 (= E149), E137 (= E151), E192 (= E214), E199 (= E221), H248 (= H270), R319 (= R340), E336 (= E357), R338 (= R359)
binding glutamine: E137 (= E151), E192 (= E214), R301 (= R322), E307 (≠ S328)
binding magnesium ion: I66 (≠ Y83), E135 (= E149), E135 (= E149), E199 (= E221), H248 (= H270), H248 (= H270), E336 (= E357), H419 (≠ T431)
binding : F63 (= F81), V64 (≠ A82), R65 (vs. gap), I66 (≠ Y83), D161 (= D183), G241 (≠ A263), V242 (≠ Q264), N243 (≠ A265), G305 (≠ N326), Y306 (≠ H327), Y376 (= Y397), I426 (≠ V438), M430 (≠ L442)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
28% identity, 96% coverage: 15:456/459 of query aligns to 8:441/444 of P12425

query
sites
P12425

D

D

A

I

K

E

A

K

W

L

L

V

T

K

R

E

H

E

G

N

I

V

N

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

G

Q

K

A

A

L

L

E

D

D

N

R

K

A

V

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G
|
G

F

F

A

V

-
x
R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

Y

P

P

D

D

V

L

S

N

T

T

L

F

C

V

L

I

A

F

P

P

-

W

G

T

A

A

A

E

P

K

G

G

K

K

A

V

Y

A

V

R

F

F

-

I

A

C

D

D

A

I

F

Y

H

N

M

P

D

D

D

G

R

T

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

N

V

N

L

L

R

K

A

R

V

I

L

L

D

K

L

E

Y

M

A

E

K

D

R

L

G

G

W

F

R

S

A

D

V

F

-

N

V

L

A

G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

L

N

D

P

E

-

K

-

G

D

E

P

P

D

T

R

L

P

E

L

L

T

N

A

D

P

K

V

G

G

G

A

Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S
x
V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

G

A

A

L

V

P

R

L

S

A

C

D

D

K

D

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

C

H

N

M

L

S

S

V

L

I

F

D

K

E

N

A

G

G

V

R

N

T

A

L

F

F

F

A

D

G

E

S

N

D

A

D

D

-

L

-

Q

-

L

A

S

D

E

T

T

A

A

M

-

F

-

G

K

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

E

S

I

F

M

T

P

A

L

V

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N
x
G

H

Y

S
x
E

A

A

P
|
P

A

C

N

Y

I

V

E

A

W

W

S

S

H

A

D

Q

N

N

R

R

S

S

C

P

G

L

L

I

R

R

V

I

P

P

A

A

G

S

G

R

R

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

L

S

P

V

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

G

V

S

L

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

N

K

K

L

L

A

E

R

A

S

P

P

A

E

P

A

I

S

D

G

R

N

N

A

I

Y

Y

K

V

I

M

K

S

S

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

K

A

T

T

M

L

E

A

E

E

A

A

L

L

D

E

R

E

F

F

E

K

A

S

C

N

G

E

P

V

V

M

R

V

E

K

L

A

L

L

G

G

E

E

D

H

F

L

F

F

Q

E

T

H

Y

F

L

I

R

E

V

A

K

K

S

E

V

I

E
|
E

L

W

D

D

L

M

F

F

Q

R

G

T

V

Q

V

V

T

H

S

P

W

W

E

E

R

R

D

E

H

Q

L

Y

L

M

G59 (= G80) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (vs. gap) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E149) binding Mg(2+)
E134 (= E151) binding Mg(2+)
E189 (= E214) binding Mg(2+)
V190 (≠ S215) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E221) binding Mg(2+)
G241 (= G266) binding L-glutamate
H245 (= H270) binding Mg(2+)
G302 (≠ N326) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ S328) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P330) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E357) binding Mg(2+)
E424 (= E439) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
28% identity, 96% coverage: 15:456/459 of query aligns to 7:440/443 of 4lnkA

query
sites
4lnkA

D

D

A

I

K

E

A

K

W

L

L

V

T

K

R

E

H

E

G

N

I

V

N

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

G

Q
x
K

A

A

L

L

E

D

D

N

R

K

A

V

L
x
M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S
x
D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

Y

P

P

D

D

V

L

S

N

T

T

L

F

C

V

L

I

A

F

P

P

G

W

A

T

A

A

-

E

P

K

G

G

K

K

A

V

Y

A

V

R

F

F

-

I

A

C

D

D

A

I

F

Y

H

N

M

P

D

D

D

G

R

T

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

N

V

N

L

L

R

K

A

R

V

I

L

L

D

K

L

E

Y

M

A

E

K

D

R

L

G

G

W

F

R

S

A

D

V

F

-

N

V

L

A

G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

L

N

D

P

E

-

K

-

G

D

E

P

P

D

T

R

L

P

E

L

L

T

N

A

D

P

K

V

G

G

G

A

Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

S
x
V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F
|
F

L

K

H
x
Y

G

A

D

G

A

A

L

V

P

R

L

S

A

C

D

D

K

D

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A
x
N

G
|
G

S

S

S
x
G

M

M

H
|
H

L

C

H
x
N

M

L

S
|
S

V

L

I

F

D

K

E

N

A

G

G

V

R

N

T

A

L

F

F

F

A

D

G

E

S

N

D

A

D

D

-

L

-

Q

-

L

A

S

D

E

T

T

A

A

M

-

F

-

G

K

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

E

S

I

F

M

T

P

A

L

V

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

C

N

Y

I

V

E

A

W

W

S

S

H

A

D

Q

N

N

R
|
R

S

S

C

P

G

L

L

I

R

R

V

I

P

P

A

A

G
x
S

G

R

R

G

A

I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

G

V

S

L

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

N

K

K

L

L

A

E

R

A

S

P

P

A

E

P

A

I

S

D

G

R

N

N

A

I

Y

Y

K

V

I

M

K

S

S

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

K

A

T

T

M

L

E

A

E

E

A

A

L

L

D

E

R

E

F

F

E

K

A

S

C

N

G

E

P

V

V

M

R

V

E

K

L

A

L

L

G

G

E

E

D

H

F

L

F

F

Q

E

T

H

Y

F

L

I

R

E

V

A

K

K

S

E

V

I

E

E

L

W

D

D

L

M

F

F

Q

R

G

T

V

Q

V

V

T

H

S

P

W

W

E

E

R

R

D

E

H

Q

L

Y

L

M

active site: D52 (≠ S74), E131 (= E149), E133 (= E151), E188 (= E214), E195 (= E221), H244 (= H270), R315 (= R340), E332 (= E357), R334 (= R359)
binding adenosine-5'-diphosphate: K43 (≠ Q51), M50 (≠ L58), F198 (= F224), Y200 (≠ H226), N246 (≠ H272), S248 (= S274), S324 (≠ G349), S328 (≠ A353), R330 (= R355)
binding glutamic acid: E133 (= E151), E188 (= E214), V189 (≠ S215), N239 (≠ A265), G240 (= G266), G242 (≠ S268), E303 (≠ S328)
binding magnesium ion: E131 (= E149), E188 (= E214), E195 (= E221), H244 (= H270), E332 (= E357)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
28% identity, 96% coverage: 15:456/459 of query aligns to 7:440/443 of 4lniA

query
sites
4lniA

D

D

A

I

K

E

A

K

W

L

L

V

T

K

R

E

H

E

G

N

I

V

N

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

I

P

P

T

V

A

S

K

Q

F

L

L

G

Q

K

A

A

L

L

E

D

D

N

R

K

A

V

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S
x
D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

Y

P

P

D

D

V

L

S

N

T

T

L

F

C

V

L

I

A

F

P

P

G

W

A

T

A

A

-

E

P

K

G

G

K

K

A

V

Y

A

V

R

F

F

-

I

A

C

D

D

A

I

F

Y

H

N

M

P

D

D

D

G

R

T

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

N

V

N

L

L

R

K

A

R

V

I

L

L

D

K

L

E

Y

M

A

E

K

D

R

L

G

G

W

F

R

S

A

D

V

F

-

N

V

L

A

G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

L

N

D

P

E

-

K

-

G

D

E

P

P

D

T

R

L

P

E

L

L

T

N

A

D

P

K

V

G

G

G

A

Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

M

G

G

L

F

P

E

L

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F

F

L

K

H
x
Y

G

A

D

G

A

A

L

V

P

R

L

S

A

C

D

D

K

D

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G

G

S

S

S

G

M

M

H
|
H

L

C

H
x
N

M

L

S
|
S

V

L

I

F

D

K

E

N

A

G

G

V

R

N

T

A

L

F

F

F

A

D

G

E

S

N

D

A

D

D

-

L

-

Q

-

L

A

S

D

E

T

T

A

A

M

-

F

-

G

K

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

E

S

I

F

M

T

P

A

L

V

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

C

N

Y

I

V

E

A

W

W

S

S

H

A

D

Q

N

N

R
|
R

S

S

C

P

G

L

L

I

R
|
R

V

I

P

P

A

A

G

S

G

R

R

G

A

I

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

G

V

S

L

L

L

L

A

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

N

K

K

L

L

A

E

R

A

S

P

P

A

E

P

A

I

S

D

G

R

N

N

A

I

Y

Y

K

V

I

M

K

S

S

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

K

A

T

T

M

L

E

A

E

E

A

A

L

L

D

E

R

E

F

F

E

K

A

S

C

N

G

E

P

V

V

M

R

V

E

K

L

A

L

L

G

G

E

E

D

H

F

L

F

F

Q

E

T

H

Y

F

L

I

R

E

V

A

K

K

S

E

V

I

E

E

L

W

D

D

L

M

F

F

Q

R

G

T

V

Q

V

V

T

H

S

P

W

W

E

E

R

R

D

E

H

Q

L

Y

L

M

active site: D52 (≠ S74), E131 (= E149), E133 (= E151), E188 (= E214), E195 (= E221), H244 (= H270), R315 (= R340), E332 (= E357), R334 (= R359)
binding adenosine-5'-diphosphate: E131 (= E149), E183 (≠ D209), D197 (≠ N223), Y200 (≠ H226), N246 (≠ H272), S248 (= S274), R320 (= R345), R330 (= R355)
binding magnesium ion: E131 (= E149), E131 (= E149), E133 (= E151), E188 (= E214), E195 (= E221), E195 (= E221), H244 (= H270), E332 (= E357)
binding l-methionine-s-sulfoximine phosphate: E133 (= E151), E188 (= E214), H244 (= H270), R297 (= R322), E303 (≠ S328), R315 (= R340), R334 (= R359)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 95% coverage: 20:457/459 of query aligns to 12:446/446 of P9WN37

query
sites
P9WN37

L

L

T

E

R

E

H

R

G

D

I

I

N

R

E

F

V

V

E

R

C

L

L

W

V

F

P

T

D

D

M

V

N

L

G

G

V

F

L

L

R

K

G

S

K

V

A

A

L

I

P

A

T

P

A

A

K

E

F

L

L

E

Q

G

A

A

L

F

E

E

D

E

R

G

A

I

L

-

Y

-

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

G

Y

F

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

A

-

R

Y

V

S

S

D

E

P

S

D

D

M

T

R

V

M

A

V

H

P

P

D

D

V

P

S

S

T

T

L

F

C

Q

L

V

A

L

P

P

G

W

A

A

A

T

P

S

G

S

-

G

-

H

K

S

A

A

Y

R

V

M

F

F

A

C

D

D

A

I

F

T

H

M

M

P

D

D

D

G

R

S

P

P

W

S

M

W

A

A

S

D

P

P

R

R

H

H

V

V

L

L

R

R

A

R

V

Q

L

L

D

T

L

K

Y

A

A

G

K

E

R

L

G

G

W

F

R

S

A

C

V

Y

V

V

A

H

P

P

E

E

V

I

E

E

F

F

Y

F

L

L

T

L

A

K

P

P

N

G

P

P

D

E

P

-

D

D

R

G

P

S

L

V

T

P

A

V

P

P

V

V

G

D

A

N

N

A

G

G

R

Y

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

Q

A

A

G

V

L

H

E

D

E

S

F

A

E

L

P

N

V

F

I

R

R

R

R

H

I

A

Y

I

D

D

Y

A

A

L

A

E

A

F

A

M

G

G

L

I

P

S

L

V

D

E

T

F

L

S

I

H

H

H

E

E

S

G

G

A

T

P

A

G

Q

Q

L

Q

E

E

I

I

N

D

F

L

L

R

H

F

G

A

D

D

A

A

L

L

P

S

L

M

A

A

D

D

K

N

V

V

L

M

L

T

F

F

K

R

R

Y

L

V

T

I

R

K

Q

E

A

V

A

A

Q

L

Q

E

S

E

G

G

M

A

H

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

F

A

G

A

Q

Q

H

A

P

G

G

S

S

S

A

M

M

H

H

L

T

H

H

M

M

S

S

V

L

I

F

D

E

E

G

A

D

G

V

R

N

T

A

L

F

F

H

A

S

G

A

S

D

D

D

D

P

A

L

D

Q

T

L

A

S

M

E

F

V

G

G

H

K

-

S

F

F

I

I

G

A

G

G

L

I

Q

L

K

E

Y

H

V

A

P

C

E

E

I

I

M

S

P

A

L

V

F

T

A

N

P

Q

N

W

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

Q

N

G

H

G

S

E

A

A

P

P

A

T

N

A

I

A

E

S

W

W

S

G

H

A

D

A

N

N

R

R

S

S

C

A

G

L

L

V

R

R

V

V

P

P

A

M

-

Y

-

T

-

P

G

H

G

K

R

T

A

S

R

R

V

V

E

E

N

V

R

R

L

S

P

P

G

D

A

S

D

A

S

C

N

N

P

P

Y

Y

L

L

A

T

I

F

A

A

G

V

S

L

L

L

L

A

A

A

G

G

Y

L

L

R

G

G

V

V

E

E

Q
x
K

K

G

L

Y

A

V

R

L

S

G

P

P

E

Q

A

A

S

E

G

D

N

N

A

V

Y

W

K

D

I

L

K

T

-

P

-

E

-

R

-

A

-

M

-

G

-

Y

S

R

T

E

L

L

P

P

K

S

T

S

M

L

E

D

E

S

A

A

L

L

D

R

R

A

F

M

E

E

A

A

C

S

G

E

P

L

V

V

R

A

E

E

L

A

L

L

G

G

E

E

D

H

F

V

F

F

Q

D

T

F

Y

F

L

L

R

R

V

N

K

K

S

R

V

T

E

E

L

W

D

A

L

N

F

Y

Q

R

G

S

V

H

V

V

T

T

S

P

W

Y

E

E

-

L

R

R

D

T

H

Y

L

L

L

S

L

L

K363 (≠ Q384) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5dm3A Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 84% coverage: 66:451/459 of query aligns to 23:368/374 of 5dm3A

query
sites
5dm3A

L

I

V

V

S

D

I

M

D

Q

G

G

R

R

Y

L

S

M

G

G

S

K

-

R

-

L

-

H

-

A

-

R

-

H

-

F

I

V

D

D

E

H

G

G

F

W

A

E

Y

E

S

T

D

H

P

C

D

I

M

M

R

K

M

-

V

-

P

P

D

D

V

L

S

A

T

T

L

L

C

R

L

C

A

V

P

P

G

W

A

L

A

-

P

E

G

G

K

T

A

A

Y

M

V

V

F

L

A

C

D

D

A

L

F

L

H

H

M

A

D

E

D

V

R

P

P

H

W

-

M

-

A

-

S

A

P

P

R

R

H

A

V

I

L

L

R

K

A

R

V

Q

L

L

D

A

L

R

Y

L

A

E

K

A

R

M

G

G

W

L

R

E

A

A

V
x
I

V

M

A

A

P

T

E
|
E

V

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

P

K

N

S

P

L

D

D

P

T

D

T

R

K

P

E

L

-

T

-

A

-

P

-

V

-

G

-

A

-

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

-

D

-

M

-

A

-

G

-

L

-

E

-

F

-

E

E

P

H

V

V

I

L

R

R

R

P

I

L

Y

R

D

N

Y

H

A

L

A

H

A

A

G

G

L

I

P

P

L

V

D
x
E

T

G

L

-

I

-

H

-

E

T

S

K

G

G

T
x
E

A

G

Q

Q

L

E

E
|
E

I

L

N

N

F

I

L
x
R

H
x
C

G

A

D

K

A

A

L

L

P

D

L

T

A

A

D

D

K

Y

V

H

L

T

L

I

F

A

K

K

R

H

L

A

T

T

R

K

Q

E

A

I

A

A

Q

W

Q

Q

S

Q

G

G

M

R

H

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

A

H

A

A

Q

H

A

A

G

G

S

S

M

S

H
|
H

L

I

H
|
H

M

Q

S
|
S

V

L

I

W

D

K

E

Q

A

-

G

G

R

L

T

P

L

A

F

F

A

H

G

D

S

E

D

R

D

D

A

A

-

L

-

G

D

M

T

S

A

A

M

L

F

M

G

K

H

H

F

Y

I

L

G

A

G

G

L

L

Q

L

K

K

Y

Y

V

A

P

P

E

D

I

Y

M

T

P

Y

L

F

F

L

A

A

P

P

N

Y

V

L

N

N

S

S

F

Y

R

K

R

R

I

F

R

Q

P

P

N

T

H

R

S

T

A

V

P

-

A

-

N

-

I

-

E

-

W

W

S

S

H

V

D

D

N

N

R
|
R

S

T

C

A

G

G

L

F

R

R

V

L

P

C

A

A

G

E

G

G

R

T

A

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

L

I

P

G

G

G

A

S

D

D

S

L

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

G

G

S

Q

L

L

L

A

A

A

G

G

Y

I

L

K

G

G

V

I

E

E

Q

E

K

C

L

L

A

A

R

L

S

P

P

P

E

P

A

A

S

-

G

-

N

-

A

-

Y

-

K

-

I

-

K

-

S

G

T

L

L

I

P

P

K

Q

T

N

M

L

E

R

E

D

A

A

L

M

D

E

R

A

F

L

E

R

A

G

C

S

G

T

P

M

V

L

R

R

E

E

L

A

L

M

G

G

E

E

D

D

F

V

Q

D

T

H

Y

Y

L

V

R

R

V

A

K

A

S

E

V

V

E

E

L

L

D

E

L

D

F

F

Q

Q

G

R

V

V

T

S

S

D

W

Y

E

E

active site: E107 (= E149), E109 (= E151), E146 (≠ T217), E150 (= E221), H199 (= H270), R265 (= R340), E282 (= E357), R284 (= R359)
binding adenosine-5'-diphosphate: I103 (≠ V145), E141 (≠ D209), R154 (≠ L225), C155 (≠ H226), H201 (= H272), S203 (= S274), R280 (= R355)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
33% identity, 84% coverage: 73:456/459 of query aligns to 51:444/446 of A0R083

query
sites
A0R083

Y

F

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

A

-

R

Y

V

S

F

D

E

P

S

D

D

M

T

R

V

M

A

V

R

P

P

D

D

V

P

S

S

T

T

L

F

C

Q

L

V

A

L

P

P

-

W

G

K

A

T

A

S

P

D

G

G

K

N

A

H

Y

Y

-

S

-

A

-

R

V

M

F

F

A

C

D

D

A

I

F

T

H

M

M

P

D

D

D

G

R

S

P

P

W

S

M

W

A

A

S

D

P

S

R

R

H

H

V

V

L

L

R

R

A

R

V

Q

L

L

D

A

L

K

Y

A

A

S

K

D

R

L

G

G

W

F

R

T

A

C

V

Y

V

V

A

H

P

P

E

E

V

I

E

E

F

F

Y

F

L

L

T

L

A

K

P

P

N

G

P

P

D

N

P

D

D

G

R

T

P

P

L

-

T

-

A

-

P

P

V

E

G

P

A

A

N

D

G

N

R

G

A

G

E

Y

T

F

V

D

Q

Q

H

A

P

V

Y

H

D

D

M

A

A

A

G

-

L

-

E

-

F

-

E

-

P

P

V

N

I

F

R

R

-

H

I

A

Y

I

D

E

Y

A

A

L

A

E

A

Q

A

M

G

G

L

I

P

S

L

V

D

E

T

F

L

S

I

H

H

H

E

E

S

G

G

A

T

P

A

G

Q

Q

L

Q

E

E

I

I

N

D

F

L

L

R

H

Y

G

A

D

D

A

A

L

L

P

S

L

M

A

A

D

D

K

N

V

V

L

M

L

T

F

F

K

R

R

Y

L

L

T

V

R

K

Q

E

A

V

A

A

Q

L

Q

A

S

D

G

G

M

V

H

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

F

A

A

A

E

Q

H

A

P

G

G

S

S

S

A

M

M

H

H

L

T

H

H

M

M

S

S

V

L

I

F

D

-

E

E

A

G

G

D

R

T

T

N

L

A

F

F

A

H

G

S

S

P

D

D

-

P

-

L

-

Q

-

L

A

S

D

D

T

V

A

A

M

K

F

-

G

-

H

S

F

F

I

I

G

A

G

G

L

I

Q

L

K

E

Y

H

V

A

P

N

E

E

I

I

M

S

P

A

L

V

F

T

A

N

P

Q

N

W

V

V

N

N

S

S

F

Y

R

K

R

R

I

L

R

V

P

H

N

G

H

G

S

E

A

A

P

P

A

T

N

A

I

A

E

S

W

W

S

G

H

A

D

A

N

N

R

R

S

S

C

A

G

L

L

V

R

R

V

V

P

P

A

M

-

Y

-

T

-

P

G

H

G

K

R

V

A

S

R

R

V

V

E

E

N

V

R

R

L

S

P

P

G

D

A

S

D

A

S

C

N

N

P

P

Y

Y

L

L

A

T

I

F

A

A

G

V

S

L

L

L

L

A

A

A

G

G

Y

L

L

R

G

G

V

V

E

E

Q
x
K

K

G

L

Y

A

V

R

L

S

G

P

P

E

Q

A

A

S

E

G

D

N

N

A

V

Y

W

K

S

I

L

K

T

S

Q

-

E

-

R

-

A

-

M

-

G

-

Y

-

R

T

E

L

L

P

P

K

T

T

S

M

L

E

G

E

N

A

A

L

L

D

E

R

S

F

M

E

E

A

N

C

S

G

E

P

L

V

V

R

A

E

E

L

A

L

L

G

G

E

E

D

H

F

V

F

F

Q

D

T

Y

Y

F

L

L

R

R

V

N

K

K

S

R

V

S

E

E

L

W

D

E

L

N

F

Y

Q

R

G

S

V

H

V

V

T

T

S

P

W

Y

E

E

R

L

D

K

H

N

L

Y

L

L

K363 (≠ Q384) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tdvC Glutamine synthetase (see paper)
26% identity, 96% coverage: 15:456/459 of query aligns to 7:440/443 of 7tdvC

query
sites
7tdvC

D

D

A

I

K

R

A

K

W

F

L

V

T

E

R

E

H

E

G

N

I

V

N

R

E

Y

V

L

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

N

L

G

G

V

T

L

I

R

K

G

N

K

V

A

E

L

V

P

P

T

V

A

S

K

Q

F

L

L

E

Q

K

A

V

L

L

E

D

D

N

R

E

A

M

L

M

Y

F

L

-

P

-

S

-

A

-

F

-

L

-

V

-

S

-

I

-

D

-

G

-

R

-

Y

-

S

D

G

G

S

S

I

S

D

I

E

E

G

G

F

F

A

V

-

R

Y

I

S

E

D

E

P

S

D

D

M

M

R

Y

M

L

V

H

P

P

D

D

V

L

S

D

T

T

L

W

C

V

L

I

A

F

P

P

-

W

G

T

A

A

A

G

P

Q

G

G

K

K

-

V

A

A

Y

R

V

L

F

I

A

C

D

D

A

V

F

Y

H

K

M

T

D

D

D

G

R

T

P

P

W

F

M

E

A

G

S

D

P

P

R

R

H

A

V

N

L

L

R

K

A

R

V

V

L

L

D

K

L

E

Y

M

A

E

K

D

R

L

G

G

W

F

R

T

A

D

V

F

-

N

V

L

A
x
G

P

P

E
|
E

V

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

P

L

N

D

P

E

-

K

-

G

D

E

P

P

D

T

R

L

P

E

L

L

T

N

A

D

P

D

V

G

G

G

A

Y

N

-

G

-

R

-

A

-

E

-

T

-

V

-

Q

-

H

-

P

-

Y

F

D

D

M

L

A

A

G

P

L

T

E

D

E

L

F

G

E

E

P

N

V

C

I

R

R

R

R

D

I

I

Y

V

D

L

Y

E

A

L

A

E

A

D

A

M

G

G

L

F

P

D

L

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

S

V

G

A

T

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F
|
F

L
x
K

H
x
Y

G

A

D

D

A

A

L

V

P

T

L

A

A

C

D

D

K

N

V

I

L

Q

L

T

F

F

K

K

R

L

L

V

T

V

R

K

Q

T

A

I

A

A

Q

R

Q

K

S

H

G

N

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

A

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

F

H
x
N

M
x
V

S
|
S

V

L

I

F

D

K

E

G

A

K

G

E

R

N

T

A

L

F

F

F

-

D

A

P

G

N

S

T

D

E

D

M

A

G

D

L

T

T

A

E

M

T

F

A

G

Y

H

Q

F

F

I

T

G

A

G

G

L

V

Q

L

K

K

Y

N

V

A

P

R

E

G

I

F

M

T

P

A

L

V

F

C

A

N

P

P

N

L

V

V

N

N

S

S

F

Y

R

K

R
|
R

I

L

R

V

P

P

N

G

H

Y

S
x
E

A

A

P

P

A

C

N

Y

I

I

E

A

W

W

S

S

H

G

D

K

N

N

R
|
R

S

S

C

P

G

L

L

I

R
|
R

V

V

P

P

A

S

G

S

G

R

R

G

A

L

A
x
S

R

T

R
|
R

V

I

E
|
E

N

V

R

R

L

S

P

V

G

D

A

P

D

A

S

A

N

N

P

P

Y

Y

L

M

A

A

I

L

A

A

G

A

S

I

L

L

L

E

A

A

G

G

Y

L

L

D

G

G

V

I

E

K

Q

N

K

K

L

L

A

K

R

V

S

P

P

E

E

P

A

V

S

N

G

Q

N

N

A

I

Y

Y

K

E

I

M

K

N

-

R

-

E

-

R

-

E

-

A

-

V

-

G

-

I

S

Q

T

D

L

L

P

P

K

S

T

T

M

L

E

Y

E

T

A

A

L

L

D

K

R

A

F

M

E

R

A

E

C

N

G

E

P

V

V

I

R

K

E

K

L

A

L

L

G

G

E

N

D

H

F

I

F

Y

Q

N

T

Q

Y

F

L

I

R

N

V

S

K

K

S

S

V

I

E

E

L

W

D

D

L

Y

F

Y

Q

R

G

T

V

Q

V

V

T

S

S

E

W

W

E

E

R

R

D

D

H

Q

L

Y

L

M

binding adenosine-5'-diphosphate: G129 (≠ A147), E131 (= E149), E183 (≠ D209), D197 (≠ N223), F198 (= F224), K199 (≠ L225), Y200 (≠ H226), N246 (≠ H272), V247 (≠ M273), S248 (= S274), R320 (= R345), S328 (≠ A353), R330 (= R355)
binding magnesium ion: E131 (= E149), E131 (= E149), E133 (= E151), E188 (= E214), E195 (= E221), E195 (= E221), H244 (= H270), E332 (= E357)
binding l-methionine-s-sulfoximine phosphate: E131 (= E149), E133 (= E151), E188 (= E214), E195 (= E221), G240 (= G266), H244 (= H270), R297 (= R322), E303 (≠ S328), R315 (= R340)

Query Sequence

>WP_013528949.1 NCBI__GCF_000185905.1:WP_013528949.1
MPDNNTNISLSSGTDAKAWLTRHGINEVECLVPDMNGVLRGKALPTAKFLQALEDRALYL
PSSAFLVSIDGRYSGSIDEGFAYSDPDMRMVPDVSTLCLAPGAAPGKAYVFADAFHMDDR
PWMASPRHVLRAVLDLYAKRGWRAVVAPEVEFYLTAPNPDPDRPLTAPVGANGRAETVQH
PYDMAGLEEFEPVIRRIYDYAAAAGLPLDTLIHESGTAQLEINFLHGDALPLADKVLLFK
RLTRQAAQQSGMHATFMAKPIAAQAGSSMHLHMSVIDEAGRTLFAGSDDADTAMFGHFIG
GLQKYVPEIMPLFAPNVNSFRRIRPNHSAPANIEWSHDNRSCGLRVPAGGRAARRVENRL
PGADSNPYLAIAGSLLAGYLGVEQKLARSPEASGNAYKIKSTLPKTMEEALDRFEACGPV
RELLGEDFFQTYLRVKSVELDLFQGVVTSWERDHLLLKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory