SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013532939.1 NCBI__GCF_000185905.1:WP_013532939.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
31% identity, 92% coverage: 16:423/442 of query aligns to 11:425/425 of O59010

query
sites
O59010

P

P

F

V

Y

L

A

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

D

S

A

V

V

G

K

E

T

S

Y

M

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T
x
S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

A

S

V

T

G

V

G

N

Q

T

Q

Y

F

A

Q

A

P

K

K

A

-

H

Q

D

A

Q

P

S

P

V

L

T

V

G

K

F

I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

K

L

I

V

V

S

N

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V

L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S

T

M

V

G

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I
x
V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E
x
M

C

G

R
|
R

A

T

L

M

T

V

N
|
N

F

V

V

T

G

G

N
x
D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

G

G

E

E

L

L

D

E

E

K

A

G

K

V

L

I

A

A

S65 (≠ T68) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S279) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 279:281) binding L-aspartate
M311 (≠ L314) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T317) binding L-aspartate
V355 (≠ I358) binding L-aspartate
D394 (≠ S392) binding L-aspartate
M395 (≠ E393) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R395) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N399) binding L-aspartate
D405 (≠ N403) mutation to N: Strongly decreased affinity for aspartate.

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
32% identity, 88% coverage: 32:421/442 of query aligns to 25:423/426 of 6xwnB

query
sites
6xwnB

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

S

A

V

V

G

K

E

T

S

Y

M

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

I

F

G

G

L

L

V

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

F

V

N

N

I

L

D

G

P

S

A

G

T

T

L

-

D

-

A

-

S

-

T

-

V

-

N

-

T

G

Y

K

A

A

A

I

K

E

A

A

H

Q

D

P

Q

P

S

S

V

L

T

V

G

Q

F

T

L

L

M

L

N

N

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

D

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

V

L

G

M

D

N

K

R

G

N

E

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

L

L

N

R

F

V

L

F

Q

D

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

L

L

I

V

V

S

G

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

A

V

V

V

I

G

N

P

L

L

A

A

M

K

L

V

V

V

G

G

T

A

F

V

Y

Y

V

T

T

G

S

L

F

F

L

L

F

Q

V

I

F

V

I

I

V

T

L

Y

G

F

A

I

V

L

C

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S

R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

A

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

I

H

H

P

L

L

S

T

M

L

G

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A

T

L

M

T

V

N

N

F

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

R

K

W

T

E

E

G

K

E

E

L

L

D

D

E

E

A

S

K

K

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S280), S276 (= S281), M310 (≠ L314), T313 (= T317), D394 (≠ S392), R397 (= R395)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
32% identity, 88% coverage: 32:421/442 of query aligns to 25:423/427 of 5e9sA

query
sites
5e9sA

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

S

A

V

V

G

K

E

T

S

Y

M

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F
x
Y

L

L

T

A

F
x
T

S
|
S

T

A

L

M

A

A

L

V

I

F

G

G

L

L

V

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

F

V

N

N

I

L

D

G

P

S

A

G

T

T

L

-

D

-

A

-

S

-

T

-

V

-

N

-

T

G

Y

K

A

A

A

I

K

E

A

A

H

Q

D

P

Q

P

S

S

V

L

T

V

G

Q

F

T

L

L

M

L

N

N

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

D

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

V

L

G

M

D

N

K

R

G

N

E

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

L

L

N

R

F

V

L

F

Q

D

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K
x
L

L

I

V

V

S

G

V
x
G

L

V

M

M

K

Q

A
x
Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

A

V

V

V

I

G

N

P

L

L

A

A

M

K

L

V

V

V

G

G

T

A

F

V

Y

Y

V

T

T

G

S

L

F

F

L

L

F

Q

V

I

F

V

I

I

V

T

L

Y

G

F

A

I

V

L

C

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

A

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

I

H

H

P

L

L

S

T

M

L

G

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S
|
S

K
x
I

G
|
G

A
x
T

A

A

G
|
G

I
x
V

T

P

G

G

A
|
A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N
|
N

F
x
V

V

T

G

G

N
x
D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

R

K

W

T

E

E

G

K

E

E

L

L

D

D

E

E

A

S

K

K

binding aspartic acid: R274 (≠ S279), S275 (= S280), S276 (= S281), T313 (= T317), G353 (= G357), V354 (≠ I358), A357 (= A361), G358 (= G362), D394 (≠ S392), R397 (= R395), T398 (≠ A396)
binding decyl-beta-d-maltopyranoside: L194 (≠ K199), G198 (≠ V203), Y202 (≠ A207)
binding sodium ion: Y87 (≠ F93), T90 (≠ F96), S91 (= S97), S276 (= S281), G305 (= G309), A306 (≠ Y310), T307 (≠ S311), N309 (= N313), N309 (= N313), M310 (≠ L314), D311 (= D315), S348 (= S352), I349 (≠ K353), G350 (= G354), T351 (≠ A355), N401 (= N399), V402 (≠ F400), D405 (≠ N403)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
32% identity, 88% coverage: 32:421/442 of query aligns to 23:421/425 of 6zgbA

query
sites
6zgbA

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

S

A

V

V

G

K

E

T

S

Y

M

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

I

F

G

G

L

L

V

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

F

V

N

N

I

L

D

G

P

S

A

G

T

T

L

-

D

-

A

-

S

-

T

-

V

-

N

-

T

G

Y

K

A

A

A

I

K

E

A

A

H

Q

D

P

Q

P

S

S

V

L

T

V

G

Q

F

T

L

L

M

L

N

N

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

D

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

V

L

G

M

D

N

K

R

G

N

E

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

L

L

N

R

F

V

L

F

Q

D

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

L

L

I

V

V

S

G

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

A

V

V

V

I

G

N

P

L

L

A

A

M

K

L

V

V

V

G

G

T

A

F

V

Y

Y

V

T

T

G

S

L

F

F

L

L

F

Q

V

I

F

V

I

I

V

T

L

Y

G

F

A

I

V

L

C

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

A

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T

T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

I

H

H

P

L

L

S

T

M

L

G

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N

N

F

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

R

K

W

T

E

E

G

K

E

E

L

L

D

D

E

E

A

S

K

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S279), S273 (= S280), S274 (= S281), M308 (≠ L314), D392 (≠ S392), R395 (= R395), T396 (≠ A396)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
32% identity, 88% coverage: 32:421/442 of query aligns to 22:420/424 of 6zl4A

query
sites
6zl4A

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

S

A

V

V

G

K

E

T

S

Y

M

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

I

F

G

G

L

L

V

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

F

V

N

N

I

L

D

G

P

S

A

G

T

T

L

-

D

-

A

-

S

-

T

-

V

-

N

-

T

G

Y

K

A

A

A

I

K

E

A

A

H

Q

D

P

Q

P

S

S

V

L

T

V

G

Q

F

T

L

L

M

L

N

N

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

D

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

V

L

G

M

D

N

K

R

G

N

E

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

L

L

N

R

F

V

L

F

Q

D

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K
x
L

L

I

V

V

S

G

V
x
G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

A

V

V

V

I

G

N

P

L

L

A

A

M

K

L

V

V

V

G

G

T

A

F

V

Y

Y

V

T

T

G

S

L

F

F

L

L

F

Q

V

I

F

V

I

I

V

T

L

Y

G

F

A

I

V

L

C

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E
x
R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

A

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

I

H

H

P

L

L

S

T

M

L

G

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G
|
G

A
|
A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S

D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N

N

F

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

R

K

W

T

E

E

G

K

E

E

L

L

D

D

E

E

A

S

K

K

binding decyl-beta-d-maltopyranoside: L191 (≠ K199), G195 (≠ V203), R282 (≠ E290)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S279), S272 (= S280), S273 (= S281), M307 (≠ L314), T310 (= T317), G353 (= G360), A354 (= A361), R394 (= R395), T395 (≠ A396)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
32% identity, 88% coverage: 32:421/442 of query aligns to 18:412/416 of 6r7rA

query
sites
6r7rA

G

G

I

L

L

I

L

A

G

G

H
|
H

F

F

-

G

Y

Y

P

A

-

G

S

A

V

V

G

K

E

T

S

Y

M

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

I

F

G

G

L

L

V

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

F

V

N

N

I

L

D

G

P

S

A

G

T

T

L

-

D

-

A

-

S

-

T

-

V

-

N

-

T

-

Y

-

A

-

K

G

A

A

H

Q

D

P

Q

P

S

S

V

L

T

V

G

Q

F

T

L

L

M

L

N

N

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

D

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

V

L

G

M

D

N

K

R

G

N

E

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

L

L

N

R

F

V

L

F

Q

D

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

L

L

I

V

V

S

G

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

A

V

V
|
V

I

G

N

P

L

L

A
|
A

M

K

L

V

V

V

G

G

T

A

F

V

Y

Y

V

T

T

G

S

L

F

F

L

L

F

Q

V

I

F

V

I

I

V

T

L

Y

G

F

A

I

V

L

C

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

A

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

I

H

H

P

L

L

S

T

M

L

G

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A
x
T

A

A

G
|
G

I
x
V

T

P

G

G

A

A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N
|
N

F

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

R

K

W

T

E

E

G

K

E

E

L

L

D

D

E

E

A

S

K

K

binding d-aspartic acid: R263 (≠ S279), S265 (= S281), M299 (≠ L314), T302 (= T317), T340 (≠ A355), G342 (= G357), V343 (≠ I358), G347 (= G362), D383 (≠ S392), R386 (= R395), T387 (≠ A396), N390 (= N399)
binding decyl-beta-d-maltopyranoside: H23 (= H37), V212 (= V228), A216 (= A232)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
30% identity, 91% coverage: 16:417/442 of query aligns to 11:419/419 of 6x15A

query
sites
6x15A

P

P

F

V

Y

L

A

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

H

S

A

V

V

G

H

E

T

S

Y

M

V

K

K

P

P

L
x
F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

C

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D
x
P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F
x
Y

L

L

T
x
L

F
x
T

S
|
S

T

A

L
x
F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

-

S

-

T

A

V

V

N

G

T

G

Y

Q

A

Q

A

F

K

Q

A

P

H

H

D

Q

-

A

Q

P

S

P

V
x
L

T

V

G

H

F
x
I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V
x
I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K
|
K

L

I

V

V

S

N

V
x
G

L

V

M

M

K

Q

A

Y

A

A

P

P

I
|
I

G

G

A

V

F
|
F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

H

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V
x
L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I
|
I

R

K

Y

H

I

A

K

K

E

D

E

A

L
x
M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y

A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

A

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S
x
T

M
x
V

G
|
G

D

Q

Q

Q

I

L

L
x
T

L

I

L

V

L
|
L

V

T

A

A

M

V

L

L

S

A

S
|
S

K
x
I

G

G

A
x
T

A

A

G
|
G

I

V

T

P

G

G

A
|
A

G
|
G

F

A

I

I

T

M

L

L

A

C

A

M

T

V

L

L

S

H

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L
x
M

T

V

N
|
N

F
x
V

V

T

G

G

N
x
D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

G

G

E

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L49), F46 (≠ L49), P75 (≠ D78), L91 (≠ T95), F95 (≠ L99), L130 (≠ V141), I133 (≠ F144), I159 (≠ V170), Y167 (≠ L178), K196 (= K199), G200 (≠ V203), I207 (= I210), F210 (= F213), L250 (≠ V253), I262 (= I265), M269 (≠ L272), T334 (≠ S337), V335 (≠ M338), G336 (= G339), T340 (≠ L343), L343 (= L346), M399 (≠ L397)
binding aspartic acid: S277 (= S280), S278 (= S281), T314 (= T317), G354 (= G357), A358 (= A361), G359 (= G362), D394 (≠ S392), R397 (= R395), T398 (≠ A396)
binding sodium ion: Y89 (≠ F93), T92 (≠ F96), S93 (= S97), G306 (= G309), T308 (≠ S311), N310 (= N313), N310 (= N313), M311 (≠ L314), D312 (= D315), S349 (= S352), I350 (≠ K353), T352 (≠ A355), N401 (= N399), V402 (≠ F400), D405 (≠ N403)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

2nwwA Crystal structure of gltph in complex with tboa (see paper)
30% identity, 90% coverage: 16:414/442 of query aligns to 2:407/407 of 2nwwA

query
sites
2nwwA

P

P

F

V

Y

L

A

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

H

S

A

V

V

G

H

E

T

S

Y

M

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

-

S

-

T

A

V

V

N

G

T

G

Y

Q

A

Q

A

F

K

Q

A

P

H

H

D

Q

-

A

Q

P

S

P

V

L

T

V

G

H

F

I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

K

L

I

V

V

S

N

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

H

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V

L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

H

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S

T

M

V

G

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A
|
A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

H

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N
|
N

F

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S281), T305 (= T317), A349 (= A361), G350 (= G362), D385 (≠ S392), R388 (= R395), T389 (≠ A396), N392 (= N399)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
30% identity, 90% coverage: 16:414/442 of query aligns to 8:413/413 of 6x14A

query
sites
6x14A

P

P

F

V

Y

L

A

Q

Q

K

L
x
I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

H

S

A

V

V

G

H

E

T

S

Y

M

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

C

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

T

V

G
|
G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M
x
V

L

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

-

S

-

T

A

V

V

N

G

T

G

Y

Q

A

Q

A

F

K

Q

A

P

H

H

D

Q

-

A

Q

P

S

P

V

L

T

V

G

H

F

I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L
x
I

F

L

G

G

I

I

A

A

L

I

A

T

L
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K
|
K

L

I

V

V

S

N

V

G

L

V

M
|
M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

H

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V

L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

H

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S
x
T

F
x
I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

A

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S

T

M

V

G

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K
x
I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G
|
G

F

A

I

I

T

M

L

L

A

C

A

M

T

V

L

L

S

H

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L
|
L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A

T

L

M

T

V

N

N

F

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G72), V83 (≠ M90), I157 (≠ L171), Y164 (≠ L178), K193 (= K199), T305 (≠ S311), I306 (≠ F312), I347 (≠ K353)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L21), M199 (= M205), S275 (= S281), T311 (= T317), G356 (= G362), L384 (= L385), D391 (≠ S392), R394 (= R395)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
30% identity, 90% coverage: 16:415/442 of query aligns to 3:409/409 of 6bavA

query
sites
6bavA

P

P

F

V

Y

L

A

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

D

S

A

V

V

G

K

E

T

S

Y

M

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

A

S

V

T

G

V

G

N

Q

T

Q

Y

F

A

Q

A

P

K

K

A

-

H

Q

D

A

Q

P

S

P

V

L

T

V

G

K

F

I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

K

L

I

V

V

S

N

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V

L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S
x
R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y
|
Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S

T

M

V

G

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D
|
D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
x
C

A
x
T

L

M

T

V

N

N

F

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

G

G

binding benzylcysteine: R268 (≠ S279), S270 (= S281), T306 (= T317), Y309 (= Y320), D382 (= D388), D386 (≠ S392), C389 (≠ R395), T390 (≠ A396)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
30% identity, 90% coverage: 16:414/442 of query aligns to 3:408/408 of 6bauA

query
sites
6bauA

P

P

F

V

Y

L

A

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

D

S

A

V

V

G

K

E

T

S

Y

M

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

A

S

V

T

G

V

G

N

Q

T

Q

Y

F

A

Q

A

P

K

K

A

-

H

Q

D

A

Q

P

S

P

V

L

T

V

G

K

F

I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

K

L

I

V

V

S

N

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V

L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S

T

M

V

G

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A
|
A

G
|
G

I
x
V

T

P

G

G

A

A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
x
C

A
x
T

L

M

T

V

N
|
N

F

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

binding cysteine: S270 (= S281), M303 (≠ L314), T306 (= T317), A345 (= A356), G346 (= G357), V347 (≠ I358), G351 (= G362), D386 (≠ S392), C389 (≠ R395), T390 (≠ A396), N393 (= N399)

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
28% identity, 90% coverage: 16:414/442 of query aligns to 3:396/396 of 6bmiA

query
sites
6bmiA

P

P

F

V

Y

L

A

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

T

I

V

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

-

Y

Y

A

P

D

S

A

V

V

G

K

E

T

S

Y

M

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

T

V

G

G

I

A

A

A

G

S

M

I

S

S

D

P

L

-

Q

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

L

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

I

T

I

L

G

G

L

I

V

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

F

I

N

H

I

L

D

-

P

-

A

-

T

-

L

-

D

-

A

A

S

V

T

G

V

G

N

Q

T

Q

Y

F

A

Q

A

P

K

K

A

-

H

Q

D

A

Q

P

S

P

V

L

T

V

G

K

F

I

L

L

M

L

N

D

I

I

I

V

P

P

S

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

D

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

L

I

A

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

D

K

K

S

G

A

E

E

P

T

V

L

L

L

N

D

F

A

L

I

Q

N

A

G

L

L

T

A

T

E

P

A

I

M

F

Y

K

K

L

I

V

V

S

N

V

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

A

V

V

V

I

G

N

E

L

L

A

A

M

K

L

V

V

T

G

A

T

A

F

V

Y

Y

V

V

T

G

S

L

F

T

L

L

F

Q

V

I

F

L

I

L

V

V

L

Y

G

F

A

V

V

L

C

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S
x
R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

E

R

K

V

M

A

E

K

K

E

A

M

G

G

A

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

I

S

H

H

L

L

S

T

M

V

G

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

-

K

-

G

-

A

-

G

-

I

-

T

-

G

-

A

-

G

-

F

-

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R

C

A
x
T

L

M

T

V

N
|
N

F

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

R

K

W

T

E

E

binding serine: R268 (≠ S279), S270 (= S281), T306 (= T317), D374 (≠ S392), T378 (≠ A396), N381 (= N399)

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
29% identity, 88% coverage: 32:421/442 of query aligns to 33:412/412 of 7awmA

query
sites
7awmA

G

G

I

V

L

V

L

L

G

G

H

F

F

L

-

L

-

R

-

P

Y

Y

P

P

S

L

V

S

G

P

E

R

S

E

M

V

K

K

P

Y

L

F

-

A

-

F

-

P

G

G

D

E

A

L

F

L

I

M

K

R

L

M

V

L

K

K

M

M

I

L

I

I

A

L

P

P

V

L

I

I

F

V

L

S

T

S

V

L

T

I

T

T

G

G

I

L

A

A

G

S

M

L

S

-

D

D

L

A

Q

K

K

A

V
x
S

G
|
G

R

R

V

L

A
x
G

G

M

K

R

A
|
A

M
x
V

L

V

Y

Y

F
x
Y

L

M

T

S

F

T

S

T

T

I

L

I

A

A

L

V

I

V

I

L

G

G

L

I

V

I

V

L

A

V

N

L

V

I

Q

H

P

P

G

E

A

V

-

L

-

D

-

C

-

F

-

L

-

D

-

L

G

A

F

R

N

N

I

I

D

F

P

P

A

S

T

N

L

L

D

V

A

S

S

A

T

A

V

F

N

R

T

S

Y

Y

A

S

A

T

K

-

A

-

H

-

D

-

Q

Q

S

E

V

V

T

E

G

G

F

M

L

-

M

-

N

-

I

-

P

-

S

-

T

-

I

-

V

-

G

-

A

-

F

-

A

-

D

-

G

-

D

N

I

I

L

L

Q

G

V

L

L

V

F

V

F

F

S

S

V
x
M

L

V

F

F

G

G

I
x
F

A

A

L

L

A

G

L

K

V

M

G

G

D

E

K

Q

G

G

E

Q

P

L

V

L

L

V

N

D

F

F

L

F

Q

N

A

S

L

L

T

N

T

E

P

A

I

T

F

M

K

K

L

L

V

V

S

A

V

I

L

I

M

M

K

W

A

Y

A

A

P

P

I

L

G

G

A

I

F

L

G

F

A

L

M

I

A

A

F

G

T

K

I

I

-

V

-

G

G

G

K

Q

Y

L

G

G

I

M

G

Y

A

M

V

V

I

T

N

V

L

I

A

V

M

G

L

L

V

V

G

I

T

H

F

G

Y

L

V

I

T

V

S

L

F

P

L

L

F

I

V

Y

F

F

I

L

V

I

L

-

G

-

A

-

V

-

C

T

R

R

Y

K

N

N

G

P

F

F

S

V

I

F

L

I

A

A

L

-

I

-

R

-

Y

G

I

I

K

L

E

Q

E

A

L

L

L

I

L

T

V

A

L

L

G

G

T

T

S

S

S
|
S

E

S

A

A

A

T

L

L

P

P

S

I

L

T

M

F

E

K

K

C

M

L

E

E

K

E

A

N

-

N

G

G

A

V

K

D

R

K

S

R

V

I

V

T

G

R

L
x
F

V

V

I

L

P

P

T
x
V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

I

F

F

I

I

A

A

Q

Q

A

V

T

N

N

N

I

Y

H

E

L

L

S

D

M

F

G

G

D

Q

Q

I

I

I

L

T

L

I

L

S

L

I

V

T

A

A

M

T

L

A

S

A

S

S

K

I

G

G

A

A

G
|
G

I
|
I

T

P

G

Q

A
|
A

G
|
G

F

L

I

V

T

T

L

M

A

V

A

I

T

V

L

L

S

T

V

A

V

V

P

-

A

G

V

L

P

P

V

T

A

D

G

D

M

I

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

W

F

L

M

L

S
x
D

E

R

C

F

R
|
R

A
x
T

L

M

T

V

N

N

F

V

V

L

G

G

N

D

A

A

V

L

A

G

T

A

L

G

V

I

V

V

-

E

-

H

-

L

A

S

R

R

W

K

E

E

G

L

E

E

L

K

D

Q

E

D

A

A

K

E

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ V82), G89 (= G83), G92 (≠ A86), A95 (= A89), V96 (≠ M90), Y99 (≠ F93), M163 (≠ V170), F167 (≠ I174), F293 (≠ L304), V297 (≠ T308)
binding aspartic acid: S268 (= S280), S269 (= S281), T306 (= T317), G346 (= G357), I347 (= I358), A350 (= A361), G351 (= G362), D380 (≠ S392), R383 (= R395), T384 (≠ A396)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
29% identity, 88% coverage: 32:420/442 of query aligns to 25:397/397 of 5mjuA

query
sites
5mjuA

G

G

I

V

L

V

L

L

G

G

H

F

F

L

-

L

-

R

-

P

Y

Y

P

P

S

L

V

S

G

P

E

R

S

E

M

V

K

K

P

Y

L

F

-

A

-

F

-

P

G

G

D

E

A

L

F

L

I

M

K

R

L

M

V

L

K

K

M

M

I

L

I

I

A

L

P

P

V

L

I
|
I

F

V

L

S

T

S

V

L

T

I

T

T

G

G

I
x
L

A

A

G

S

M

L

S

-

D

D

L

A

Q

K

K

A

V
x
S

G
|
G

R

R

V

L

A
x
G

G

M

K

R

A

A

M

V

L

V

Y

Y

F
x
Y

L

M

T

S

F

T

S

T

T

I

L

I

A

A

L

V

I

V

I

L

G

G

L

I

V

I

V

L

A

V

N

L

V

I

Q

H

P

E

-

V

-

L

-

D

-

C

-

F

-

L

G

D

A

L

G

A

F

R

N

N

I

I

D

F

P

P

A

S

T

N

L

L

D

V

A

S

S

A

T

A

V

F

N

R

T

S

Y

Y

A

S

A

T

K

T

A

G

H

-

D

-

Q

Q

S

E

V

V

T

E

G

G

F

M

L

-

M

-

N

-

I

-

P

-

S

-

T

-

I

-

V

-

G

-

A

-

F

-

A

-

D

-

G

-

D

N

I
|
I

L

L

Q

G

V

L

L

V

F

V

F

F

S

S

V
x
M

L

V

F

F

G

G

I
x
F

A

A

L

L

A

G

L

K

V

M

G

G

D

E

K

Q

G

G

E

Q

P

L

V

L

L

V

N

D

F

F

L

F

Q

N

A

S

L

L

T

N

T

E

P

A

I

T

F

M

K

K

L

L

V

V

S

A

V

I

L

I

M

M

K

W

A

Y

A

A

P

P

I

L

G

G

A

I

F

L

G

F

A

L

M

I

A

A

F

G

T

K

I

I

-

V

-

E

G

G

K

Q

Y

L

G

G

I

M

G

Y

A

M

V

V

I

T

N

V

L

I

A

V

M

G

L

L

V

V

G

I

T

H

F

G

Y

L

V

I

T

V

S

L

F

P

L

L

F

I

V

Y

F

F

I

L

V

-

L

-

G

-

A

-

V

I

C

T

R

R

Y

K

N

N

G

P

F

F

S

V

I

F

L

I

A

A

L

-

I

-

R

-

Y

G

I

I

K

L

E

Q

E

A

L

L

L

I

L

T

V

A

L

L

G

G

T

T

S

S

S
|
S

E
x
S

A

A

A

T

L

L

P

P

S

I

L

T

M

F

E

K

K

C

M

L

E

E

K

E

-

N

A

N

G

G

A

V

K

D

R

K

S

R

V

I

V

T

G

R

L
x
F

V

V

I

L

P

P

T
x
V

G

G

Y
x
A

S
x
T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

I

F

F

I

I

A

A

Q

Q

A

V

T

N

N

Q

I

I

H

-

L

-

S

-

M

-

G

-

D

-

Q

-

I

I

L

T

L

I

L

S

L

I

V

T

A

A

M

T

L

A

S

A

S

S

K

I

G
|
G

A

A

G

G

I

I

T

P

G

Q

A
|
A

G
|
G

F

L

I

V

T

T

L

M

A

V

A

I

T

V

L

L

S

T

V

A

V

V

P

-

A

G

V

L

P

P

V

T

A

D

G

D

M

I

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

W

F

L

M

L

S
x
D

E

R

C

F

R
|
R

A

T

L

M

T

V

N
|
N

F

V

V

L

G

G

N

D

A

A

V

L

A

G

T

A

L

G

V

I

V

V

-

E

-

H

-

L

A

S

R

R

W

K

E

E

G

L

E

E

L

K

D

Q

E

D

A

A

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I73), S80 (≠ V82), G81 (= G83), G84 (≠ A86), Y91 (≠ F93), M156 (≠ V170), F160 (≠ I174), F286 (≠ L304), V290 (≠ T308)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (= I65), I148 (= I162), S262 (= S281), S263 (≠ E282), A292 (≠ Y310), T293 (≠ S311), M296 (≠ L314), T299 (= T317), G329 (= G354), A336 (= A361), G337 (= G362), D366 (≠ S392), R369 (= R395), N373 (= N399)

7xr4A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
29% identity, 83% coverage: 50:418/442 of query aligns to 46:420/425 of 7xr4A

query
sites
7xr4A

G

G

D

D

A

I

F

L

I

M

K

R

L

M

V

L

K

K

M

M

I

L

I

I

A

L

P

P

V

L

I

I

F

I

L

S

T

S

V

L

T

I

T

T

G

G

I

L

A

S

G

G

M

L

S

-

D

D

L

A

Q

K

K

A

V

S

G

G

R
|
R

V

L

A

G

G

T

K

R

A

A

M

M

L

V

Y

Y

F

Y

L

M

T

S

F

T

S

T

T

I

L

I

A

A

L

A

I

V

I

L

G

G

L

V

V

I

V

L

A

V

N

L

V

A

V

I

Q

H

P

P

G

G

A

-

G

-

F

-

N

N

I

P

D

K

P

V

A

S

T

S

L

L

D

D

A

A

-

F

-

L

S

D

T

L

V

I

N

R

T

N

Y

L

A

F

A

P

K

E

A

N

H

L

D

V

Q

Q

S

A

V

C

T

F

G

Q

F

Q

L

I

M

Q

N

T

I

V

I

T

P

K

S

K

T

V

I

V

V

I

G

K

A

K

-

G

-

L

-

E

F

F

A

K

D

D

G

G

-

M

D

N

I

V

L

L

Q

G

V

L

L

I

F

G

F

F

S

F

V

I

L

A

F

F

G

G

I

I

A

A

L

M

A

G

L

K

V

M

G

G

D

D

K

Q

G

A

E

K

P

L

V

M

L

V

N

D

F

F

L

F

Q

N

A

I

L

L

T

N

T

E

P

I

I

V

F

M

K

K

L

L

V

V

S

I

V

M

L

I

M

M

K

W

A

Y

A

S

P

P

I

L

G

G

A

I

F

-

G

-

A

-

M

A

A

C

F

L

T

I

I

C

G

G

K

K

Y

-

G

-

I

I

G

I

A

A

V

I

I

K

N

D

L

L

-

E

-

V

A

A

M

R

L

Q

V

L

G

G

T

M

F

Y

Y

M

V

V

T

T

-

V

-

I

-

G

-

L

-

I

-

H

S

G

F

G

L

I

F

F

V

L

F

P

I

L

V
x
I

L

Y

G

F

A

V

V

V

C

T

R

R

Y

K

N

N

G

P

F

F

S

S

I

F

L

F

A

A

L

-

I

-

R

-

Y

G

I

I

K

F

E

Q

E

A

L

W

L

I

L

T

V

A

L

L

G

G

T

T

S

A

S

S

S
|
S

E

A

A

G

A

T

L

L

P

P

S

V

L

T

M

F

E

R

K

C

M

L

E

E

K

E

A

N

-

L

G

G

A

I

K

D

R

K

S

R

V

V

V

T

G

R

L

F

V

V

I

L

P

P

T

V

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

I

F

F

I

I

A

A

Q

Q

A

M

T

N

N

G

I

V

H

V

L

L

S

D

M

G

G

G

D

Q

Q

I

I

V

L

T

L

V

L

S

L

L

V

T

A

A

M

T

L

L

S

A

S

S

K

V

G

G

A

A

G
x
S

I

I

T

P

G

S

A

A

G
|
G

F

L

I

V

T

T

L

M

A

L

T

I

L

L

S

T

V

A

V

V

P

-

A

G

V

L

P

P

V

T

A

E

G

D

M

I

A

S

L

L

I

L

L

V

G

A

V

V

D

D

R

W

F

L

M

L

S
x
D

E

R

C

M

R
|
R

A
x
T

L

S

T

V

N
|
N

F

V

V

V

G

G

N

D

A

S

V

F

-

G

A

A

T

G

L

I

V

V

V

Y

A

H

R

L

W

S

E

K

G

S

E

E

L

L

D

D

binding glutamic acid: S282 (= S281), M316 (≠ L314), T319 (= T317), S359 (≠ G357), G364 (= G362), D393 (≠ S392), R396 (= R395), T397 (≠ A396), N400 (= N399)
binding cholesterol hemisuccinate: R79 (= R84), I253 (≠ V249)

7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
29% identity, 83% coverage: 50:418/442 of query aligns to 47:419/424 of 7xr6A

query
sites
7xr6A

G

G

D

D

A

I

F

L

I

M

K

R

L

M

V

L

K

K

M

M

I

L

I

I

A

L

P

P

V

L

I

I

F

I

L

S

T

S

V

L

T

I

T

T

G

G

I

L

A

S

G

G

M

L

S

-

D

D

L

A

Q

K

K

A

V

S

G

G

R
|
R

V

L

A

G

G

T

K
x
R

A

A

M

M

L

V

Y

Y

F

Y

L

M

T

S

F

T

S

T

T

I

L
x
I

A

A

L

A

I

V

I

L

G

G

L

V

V

I

V

L

A

V

N

L

V

A

V

I

Q

H

P

P

G

G

A

-

G

-

F

-

N

N

I

P

D

K

P

V

A

S

T

S

L

L

D

D

A

A

S

-

T

-

V

-

N

-

T

-

Y

-

A

-

K

-

A

-

H

-

D

-

Q

-

S

-

V

F

T

L

G

D

F

L

L

I

M

R

N

N

I

L

I

F

P

P

S

E

T

N

I

L

V

V

G

Q

A

A

-

C

-

F

-

Q

-

I

-

Q

-

T

-

V

-

T

-

K

-

V

-

I

-

K

-

G

-

L

-

E

F

F

A

K

D

D

G

G

-

M

D

N

I

V

L

L

Q

G

V

L

L

I

F

G

F

F

S

F

V

I

L

A

F

F

G

G

I

I

A

A

L

M

A

G

L

K

V

M

G

G

D

D

K

Q

G

A

E

K

P

L

V

M

L

V

N

D

F

F

L

F

Q

N

A

I

L

L

T

N

T

E

P

I

I

V

F

M

K

K

L

L

V

V

S

I

V

M

L

I

M

M

K

W

A

Y

A

S

P

P

I

L

G

G

A

I

F

-

G

-

A

-

M

A

A

C

F

L

T

I

I

C

G

G

K

K

Y

-

G

-

I

I

G

I

A

A

V

I

I

K

N

D

L

L

-

E

-

V

A

A

M

R

L

Q

V

L

G

G

T

M

F

Y

Y

M

V

V

T

T

-

V

-

I

-

G

-

L

-

I

-

H

S

G

F

G

L

I

F

F

V

L

F

P

I

L

V
x
I

L

Y

G

F

A

V

V

V

C

T

R

R

Y

K

N

N

G

P

F

F

S

S

I

F

L

F

A

A

L

-

I

-

R

-

Y

G

I

I

K

F

E

Q

E

A

L

W

L

I

L

T

V

A

L

L

G

G

T

T

S

A

S
|
S

E

A

A

G

A

T

L

L

P

P

S

V

L

T

M

F

E

R

K

C

M

L

E

E

K

E

A

N

-

L

G

G

A

I

K

D

R

K

S

R

V

V

V

T

G

R

L

F

V

V

I

L

P

P

T

V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

I

F

F

I

I

A

A

Q

Q

A

M

T

N

N

G

I

V

H

V

L

L

S

D

M

G

G

G

D

Q

Q

I

I

V

L

T

L

V

L

S

L

L

V

T

A

A

M

T

L

L

S

A

S

S

K

V

G

G

A

A

G

S

I

I

T

P

G

S

A

A

G
|
G

F

L

I

V

T

T

L
x
M

A

L

T

I

L

L

S

T

V

A

V

V

P

-

A

G

V

L

P

P

V

T

A

E

G

D

M

I

A

S

L

L

I

L

L
x
V

G

A

V

V

D
|
D

R
x
W

F

L

M

L

S

D

E

R

C

M

R
|
R

A
x
T

L

S

T

V

N

N

F

V

V

V

G

G

N

D

A

S

V

F

-

G

A

A

T

G

L

I

V

V

V

Y

A

H

R

L

W

S

E

K

G

S

E

E

L

L

D

D

binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S280), S281 (= S281), T318 (= T317), G363 (= G362), M367 (≠ L366), V385 (≠ L385), D388 (= D388), R395 (= R395), T396 (≠ A396)
binding dodecyl beta-D-glucopyranoside: W389 (≠ R389)
binding cholesterol hemisuccinate: R80 (= R84), R84 (≠ K88), I95 (≠ L99), I252 (≠ V249)

Sites not aligning to the query:

binding dodecyl beta-D-glucopyranoside: 16, 19, 20

7vr7A Inward-facing structure of human eaat2 in the way213613-bound state (see paper)
29% identity, 80% coverage: 50:404/442 of query aligns to 39:389/402 of 7vr7A

query
sites
7vr7A

G

G

D

D

A

I

F

L

I

M

K

R

L

M

V

L

K

K

M

M

I

L

I

I

A

L

P

P

V

L

I

I

F

I

L

S

T
x
S

V
x
L

T

I

T

T

G

G

I

L

A

S

G

G

M
x
L

S

D

D

-

L

-

Q

-

K

-

V

-

G

G

R

R

V

L

A

G

G

T

K

R

A

A

M

M

L

V

Y

Y

F

Y

L

M

T

S

F

T

S

T

T

I

L

I

A

A

L

A

I

V

I

L

G

G

L

V

V

I

V

L

A

V

N

L

V

A

V

I

Q

H

P

P

G

G

A

-

G

-

F

-

N

-

I

-

D

N

P

P

A

S

T

S

L

L

D

D

A

A

S

-

T

-

V

-

N

-

T

-

Y

-

A

-

K

-

A

-

H

-

D

-

Q

-

S

-

V

F

T

L

G

D

F

L

L

I

M

R

N

N

I

L

I

F

P

P

S

E

T

N

I

L

V

V

G

Q

A

A

-

C

-

F

-

Q

-

I

-

Q

-

T

-

V

-

T

-

K

-

I

-

K

-

G

-

L

-

E

F

F

A

K

D

D

G

G

-

M

D

N

I

V

L

L

Q

G

V

L

L

I

F

G

F

F

S

F

V

I

L

A

F

F

G

G

I

I

A

A

L

M

A

G

L

K

V

M

G

G

D

D

K

Q

G

A

E

K

P

L

V

M

L

V

N

D

F

F

L

F

Q

N

A

I

L

L

T

N

T

E

P

I

I

V

F

M

K

K

L

L

V

V

S

I

V

M

L

I

M

M

K
x
W

A

Y

A

S

P

P

I

L

G

G

A

I

F

-

G

-

A

-

M

A

A

C

F

L

T

I

I

C

G

G

K

K

Y

-

G

-

I

I

G

I

A

A

V

I

I

K

N

D

L

L

-

E

-

V

A

A

M

R

L

Q

V

L

G

G

T

M

F

Y

Y

M

V

V

T

T

-

V

-

I

-

G

-

L

-
x
I

-

I

-

H

S

G

F

G

L

I

F

F

V

L

F

P

I

L

V

I

L

Y

G

F

A

V

V

V

C

T

R

R

Y

K

N

N

G

P

F

F

S

S

I

F

L
x
F

A

A

L

-

I

-

R

-

Y

G

I

I

K

F

E

Q

E

A

L
x
W

L

I

L

T

V

A

L

L

G

G

T

T

S

A

S

S

S
|
S

E

A

A

G

A

T

L

L

P

P

S

V

L

T

M

F

E

R

K

C

M

L

E

E

K

E

A

N

-

L

G

G

A

I

K

D

R

K

S

R

V

V

V

T

G

R

L

F

V

V

I

L

P

P

T

V

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y
|
Y

M

E

T

A

L

V

A

A

L

I

F

F

I

I

A

A

Q

Q

A

M

T

N

N

G

I

V

H

V

L

L

S

D

M

G

G

G

D

Q

Q

I

I

V

L

T

L

V

L

S

L

L

V

T

A

A

M

T

L

L

S

A

S

S

K
x
V

G
|
G

A

A

G
x
S

I
|
I

T

P

G

S

A

A

G
|
G

F
x
L

I

V

T

T

L
x
M

A

L

T

I

L

L

S

T

V

A

V

V

P

-

A

G

V

L

P

P

V

T

A

E

G

D

M
x
I

A

S

L

L

I
x
L

L
x
V

G

A

V

V

D
|
D

R

W

F

L

M

L

S
x
D

E

R

C
x
M

R
|
R

A
x
T

L
x
S

T

V

N
|
N

F

V

V

V

G

G

N

D

A

S

binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: S57 (≠ T68), L58 (≠ V69), L65 (≠ M76), V339 (≠ K353), G340 (= G354), S343 (≠ G357), I344 (= I358)
binding cholesterol: W188 (≠ K206), I227 (vs. gap), F250 (≠ L262), W257 (≠ L272), M379 (≠ C394), S382 (≠ L397)
binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S266 (= S281), M300 (≠ L314), T303 (= T317), Y306 (= Y320), G348 (= G362), L349 (≠ F363), M352 (≠ L366), I366 (≠ M381), L369 (≠ I384), V370 (≠ L385), D373 (= D388), D377 (≠ S392), R380 (= R395), T381 (≠ A396), N384 (= N399)

Sites not aligning to the query:

binding cholesterol: 2

8cuaA Human excitatory amino acid transporter 3 (eaat3) with bound potassium in an intermediate outward facing state (see paper)
27% identity, 81% coverage: 68:423/442 of query aligns to 61:415/416 of 8cuaA

query
sites
8cuaA

T

S

V

M

T

I

T

T

G

G

I

V

A

A

G

A

M

L

S

-

D

D

L

S

Q

N

K

V

V

S

G

G

R

K

V

I

A

G

G

L

K

R

A

A

M

V

L

V

Y

Y

F

Y

L

F

T

A

F

T

S

T

T

L

L

I

A

A

L

V

I

I

I

L

G

G

L

I

V

V

V

L

A

V

N

V

V

S

V

I

Q

K

P

P

G

G

A

S

G

T

F

V

-

D

-

A

-

M

-

L

-

D

-

L

-

I

-

R

N

N

I

M

D

F

P

P

A

E

T

N

L

L

D

V

A

Q

S

A

T

A

V

F

N

Q

T

Q

Y

Y

A

K

A

T

K

K

A

R

H

E

D

E

Q

Y

S

K

V

-

T

-

G

-

F

-

L

-

M

-

N

-

I

-

P

-

S

-

T

-

I

I

V

V

G

G

A

M

F

Y

A

S

D

D

G

G

-

I

D

N

I

V

L

L

Q

G

V

L

L

I

F

V

F

F

S

A

V

L

L

V

F

F

G

G

I

L

A

V

L

I

A

G

L

K

V

M

G

G

D

E

K

K

G

G

E

Q

P

I

V

L

L

V

N

D

F

F

L

F

Q

N

A

A

L

L

T

S

T

D

P

A

I

T

F

M

K

K

L

I

V

V

S

Q

V

I

L

I

M

M

K

W

A

Y

A

M

P

P

I

L

G

G

A

I

F

L

G

F

A

L

M

I

A

A

-

G

-

C

-

I

F

I

T

E

I

V

G

E

K

D

Y

W

G

E

I

I

G

F

A

R

V

K

I

L

N

G

L

L

A

Y

M

M

-

A

-

T

L

V

V

L

G

T

T

G

F

L

Y

A

V

I

T

H

S

S

F

I

L

V

F

I

V

L

F

P

I

L

V

I

L

Y

G

F

A

I

V

V

C

V

R

R

Y

K

N

N

G

P

F

F

S

-

I

-

L

-

A

-

L

-

I

-

R

R

Y

F

-

A

-

M

-

G

I

M

K

A

E

Q

E

A

L

L

L

T

V

A

L

L

G

M

T

I

S
|
S

S

S

E

S

A

A

A

T

L

L

P

P

S

V

L

T

M

F

E

R

K

C

M

A

E

E

K

E

A

N

G

N

-

Q

A

V

K

D

R

K

S

R

V

I

V

T

G

R

L

F

V

V

I

L

P

P

T

V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T

T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

V

F

F

I

I

A

A

Q

Q

A

L

T

N

N

D

I

L

H

D

L

L

S

G

M

I

G

G

D

Q

Q

I

I

I

L

T

L

I

L

S

L

I

V

T

A

A

M

T

L

S

S

A

S

S

K

I

G

G

A
|
A

G
|
G

I
x
V

T
x
P

G

Q

A
|
A

G

G

F

L

I

V

T

T

L

M

A

V

A

I

T

V

L

L

S

S

V

A

V

V

P

-

A

G

V

L

P

P

V

A

A

E

G

D

M

V

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

C

F

L

M

L

S
x
D

E

R

C

F

R

R

A
x
T

L

M

T

V

N

N

F

V

V

L

G

G

N

D

A

A

V

F

A

G

T

T

L

G

V

I

V

V

A

E

R

K

W

L

-

S

E

K

G

K

E

E

L

L

D

E

E

Q

A

M

K

D

L

V

A

S

binding potassium ion: S270 (= S279), S271 (= S280), A347 (= A355), A348 (= A356), G349 (= G357), V350 (≠ I358), P351 (≠ T359), A353 (= A361), D383 (≠ S392), T387 (≠ A396)

9d67A Human excitatory amino acid transporter 3 (eaat3) with bound d- aspartate in an intermediate outward facing state (see paper)
27% identity, 78% coverage: 68:412/442 of query aligns to 58:400/406 of 9d67A

query
sites
9d67A

T

S

V

M

T

I

T

T

G

G

I

V

A

A

G

A

M

L

S

-

D

D

L

S

Q

N

K

V

V

S

G

G

R

K

V

I

A

G

G

L

K

R

A

A

M

V

L

V

Y

Y

F
x
Y

L

F

T

A

F
x
T

S
x
T

T

L

L

I

A

A

L

V

I

I

I

L

G

G

L

I

V

V

V

L

A

V

N

V

V

S

V

I

Q

K

P

P

G

G

A

S

G

T

F

V

-

D

-

A

-

M

-

L

-

D

-

L

-

I

-

R

N

N

I

M

D

F

P

P

A

E

T

N

L

L

D

V

A

Q

S

A

T

A

V

F

N

Q

T

Q

Y

Y

A

K

A

T

K

K

A

R

H

E

D

E

Q

Y

S

K

V

-

T

-

G

-

F

-

L

-

M

-

N

-

I

-

P

-

S

-

T

-

I

I

V

V

G

G

A

M

F

Y

A

S

D

D

G

G

-

I

D

N

I

V

L

L

Q

G

V

L

L

I

F

V

F

F

S

A

V

L

L

V

F

F

G

G

I

L

A

V

L

I

A

G

L

K

V

M

G

G

D

E

K

K

G

G

E

Q

P

I

V

L

L

V

N

D

F

F

L

F

Q

N

A

A

L

L

T

S

T

D

P

A

I

T

F

M

K

K

L

I

V

V

S

Q

V

I

L

I

M

M

K

W

A

Y

A

M

P

P

I

L

G

G

A

I

F

L

G

F

A

L

M

I

A

A

-

G

-

C

-

I

F

I

T

E

I

V

G

E

K

D

Y

W

G

E

I

I

G

F

A

R

V

K

I

L

N

G

L

L

A

Y

M

M

-

A

-

T

L

V

V

L

G

T

T

G

F

L

Y

A

V

I

T

H

S

S

F

I

L

V

F

I

V

L

F

P

I

L

V

I

L

Y

G

F

A

I

V

V

C

V

R

R

Y

K

N

N

G

P

F

F

S

-

I

-

L

-

A

-

L

-

I

-

R

R

Y

F

-

A

-

M

-

G

I

M

K

A

E

Q

E

A

L

L

L

T

V

A

L

L

G

M

T

I

S

S

S
|
S

E

S

A

A

A

T

L

L

P

P

S

V

L

T

M

F

E

R

K

C

M

A

E

E

K

E

A

N

G

N

-

Q

A

V

K

D

R

K

S

R

V

I

V

T

G

R

L

F

V

V

I

L

P

P

T

V

G
|
G

Y
x
A

S
x
T

F
x
I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

V

F

F

I

I

A

A

Q

Q

A

L

T

N

N

D

I

L

H

D

L

L

S

G

M

I

G

G

D

Q

Q

I

I

I

L

T

L

I

L

S

L

I

V

T

A

A

M

T

L

S

S

A

S
|
S

K
x
I

G
|
G

A
|
A

A

A

G
|
G

I
x
V

T

P

G

Q

A

A

G
|
G

F

L

I

V

T

T

L

M

A

V

A

I

T

V

L

L

S

S

V

A

V

V

P

-

A

G

V

L

P

P

V

A

A

E

G

D

M

V

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

C

F

L

M

L

S
x
D

E

R

C

F

R
|
R

A
x
T

L

M

T

V

N
|
N

F

V

V

L

G

G

N
x
D

A

A

V

F

A

G

T

T

L

G

V

I

V

V

A

E

R

K

binding d-aspartic acid: S268 (= S280), S269 (= S281), T306 (= T317), G346 (= G357), V347 (≠ I358), G351 (= G362), D380 (≠ S392), R383 (= R395), T384 (≠ A396), N387 (= N399)
binding sodium ion: Y82 (≠ F93), T85 (≠ F96), T86 (≠ S97), S269 (= S281), G298 (= G309), A299 (≠ Y310), T300 (≠ S311), I301 (≠ F312), N302 (= N313), N302 (= N313), M303 (≠ L314), M303 (≠ L314), D304 (= D315), S341 (= S352), I342 (≠ K353), G343 (= G354), A344 (= A355), N387 (= N399), D391 (≠ N403)

8ctcA Human excitatory amino acid transporter 3 (eaat3) with bound glutamate in an intermediate outward facing state (see paper)
27% identity, 78% coverage: 68:412/442 of query aligns to 58:400/406 of 8ctcA

query
sites
8ctcA

T

S

V

M

T

I

T

T

G

G

I

V

A

A

G

A

M

L

S

-

D

D

L

S

Q

N

K

V

V

S

G

G

R

K

V

I

A

G

G

L

K

R

A

A

M

V

L

V

Y

Y

F
x
Y

L

F

T

A

F
x
T

S
x
T

T

L

L

I

A

A

L

V

I

I

I

L

G

G

L

I

V

V

V

L

A

V

N

V

V

S

V

I

Q

K

P

P

G

G

A

S

G

T

F

V

-

D

-

A

-

M

-

L

-

D

-

L

-

I

-

R

N

N

I

M

D

F

P

P

A

E

T

N

L

L

D

V

A

Q

S

A

T

A

V

F

N

Q

T

Q

Y

Y

A

K

A

T

K

K

A

R

H

E

D

E

Q

Y

S

K

V

-

T

-

G

-

F

-

L

-

M

-

N

-

I

-

P

-

S

-

T

-

I

I

V

V

G

G

A

M

F

Y

A

S

D

D

G

G

-

I

D

N

I

V

L

L

Q

G

V

L

L

I

F

V

F

F

S

A

V

L

L

V

F

F

G

G

I

L

A

V

L

I

A

G

L

K

V

M

G

G

D

E

K

K

G

G

E

Q

P

I

V

L

L

V

N

D

F

F

L

F

Q

N

A

A

L

L

T

S

T

D

P

A

I

T

F

M

K

K

L

I

V

V

S

Q

V

I

L

I

M

M

K

W

A

Y

A

M

P

P

I

L

G

G

A

I

F

L

G

F

A

L

M

I

A

A

-

G

-

C

-

I

F

I

T

E

I

V

G

E

K

D

Y

W

G

E

I

I

G

F

A

R

V

K

I

L

N

G

L

L

A

Y

M

M

-

A

-

T

L

V

V

L

G

T

T

G

F

L

Y

A

V

I

T

H

S

S

F

I

L

V

F

I

V

L

F

P

I

L

V

I

L

Y

G

F

A

I

V

V

C

V

R

R

Y

K

N

N

G

P

F

F

S

-

I

-

L

-

A

-

L

-

I

-

R

R

Y

F

-

A

-

M

-

G

I

M

K

A

E

Q

E

A

L

L

L

T

V

A

L

L

G

M

T

I

S

S

S
|
S

E

S

A

A

A

T

L

L

P

P

S

V

L

T

M

F

E

R

K

C

M

A

E

E

K

E

A

N

G

N

-

Q

A

V

K

D

R

K

S

R

V

I

V

T

G

R

L

F

V

V

I

L

P

P

T

V

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

N

A

I

L

Y

Y

M

E

T

A

L

V

A

A

L

V

F

F

I

I

A

A

Q

Q

A

L

T

N

N

D

I

L

H

D

L

L

S

G

M

I

G

G

D

Q

Q

I

I

I

L

T

L

I

L

S

L

I

V

T

A

A

M

T

L

S

S

A

S
|
S

K
x
I

G
|
G

A
|
A

A

A

G
|
G

I

V

T

P

G

Q

A
|
A

G

G

F

L

I

V

T

T

L

M

A

V

A

I

T

V

L

L

S

S

V

A

V

V

P

-

A

G

V

L

P

P

V

A

A

E

G

D

M

V

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

C

F

L

M

L

S
x
D

E

R

C

F

R
|
R

A

T

L

M

T

V

N
|
N

F

V

V

L

G

G

N
x
D

A

A

V

F

A

G

T

T

L

G

V

I

V

V

A

E

R

K

binding glutamic acid: S268 (= S280), S269 (= S281), M303 (≠ L314), T306 (= T317), G346 (= G357), A350 (= A361), D380 (≠ S392), R383 (= R395)
binding sodium ion: Y82 (≠ F93), T85 (≠ F96), T86 (≠ S97), S269 (= S281), G298 (= G309), A299 (≠ Y310), T300 (≠ S311), N302 (= N313), N302 (= N313), M303 (≠ L314), D304 (= D315), S341 (= S352), I342 (≠ K353), G343 (= G354), A344 (= A355), N387 (= N399), D391 (≠ N403)

Query Sequence

>WP_013532939.1 NCBI__GCF_000185905.1:WP_013532939.1
MHIADQSRALAAQRKPFYAQLYVQVLVAITVGILLGHFYPSVGESMKPLGDAFIKLVKMI
IAPVIFLTVTTGIAGMSDLQKVGRVAGKAMLYFLTFSTLALIIGLVVANVVQPGAGFNID
PATLDASTVNTYAAKAHDQSVTGFLMNIIPSTIVGAFADGDILQVLFFSVLFGIALALVG
DKGEPVLNFLQALTTPIFKLVSVLMKAAPIGAFGAMAFTIGKYGIGAVINLAMLVGTFYV
TSFLFVFIVLGAVCRYNGFSILALIRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRS
VVGLVIPTGYSFNLDGTNIYMTLAALFIAQATNIHLSMGDQILLLLVAMLSSKGAAGITG
AGFITLAATLSVVPAVPVAGMALILGVDRFMSECRALTNFVGNAVATLVVARWEGELDEA
KLARALAGTADDSLPADVVPAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory