SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013537108.1 NCBI__GCF_000185805.1:WP_013537108.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
58% identity, 100% coverage: 2:338/338 of query aligns to 3:346/346 of Q04797

query
sites
Q04797

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S98 (= S97) modified: Phosphoserine
Y146 (≠ A145) modified: Phosphotyrosine

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
55% identity, 98% coverage: 4:333/338 of query aligns to 5:333/337 of P23247

query
sites
P23247

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C132 (= C129) active site, Acyl-thioester intermediate

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
55% identity, 98% coverage: 4:333/338 of query aligns to 4:332/336 of 2r00C

query
sites
2r00C

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active site: C131 (= C129), Q158 (= Q156), R237 (= R236), H244 (= H243)
binding 2,2'-oxydiacetic acid: V15 (= V15), N95 (= N95), C131 (= C129), N315 (= N316)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 4r54A

query
sites
4r54A

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F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G74), S73 (≠ G75), T94 (≠ S96), S95 (= S97), R98 (= R100), K222 (= K214)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ R77), N93 (= N95), T94 (≠ S96), N126 (= N128), C127 (= C129), G160 (= G162), G328 (= G320)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 4r41A

query
sites
4r41A

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

D

S

R

T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

A

A

G
|
G

A
x
M

Q
x
G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S72), G72 (= G74), S73 (≠ G75), N93 (= N95), T94 (≠ S96), S95 (= S97), R98 (= R100), K222 (= K214)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), A71 (= A73), G160 (= G162), M161 (≠ A163), G162 (≠ Q164)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 4r3nA

query
sites
4r3nA

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R

R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

D

S

R

T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

active site: C127 (= C129), Q154 (= Q156), R244 (= R236), H251 (= H243)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G75), T94 (≠ S96), S95 (= S97), R98 (= R100), N126 (= N128), K222 (= K214)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), N93 (= N95), T94 (≠ S96), N126 (= N128), C127 (= C129), G160 (= G162), M161 (≠ A163), G328 (= G320)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 3q1lA

query
sites
3q1lA

G

G

Y

Y

T

T

V

V

A

A

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A

A

S

S

A

A

R

R

S

S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L

T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

D

S

R

T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G

G

A

M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K

K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

active site: C127 (= C129), Q154 (= Q156), R244 (= R236), H251 (= H243)
binding 2-amino-ethanethiol: N126 (= N128), C127 (= C129), G158 (= G160), E219 (= E211)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 3pwsA

query
sites
3pwsA

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I
|
I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding (2R)-2-aminohexanedioic acid: R98 (= R100), N126 (= N128), G158 (= G160), I208 (= I200), E219 (= E211), K222 (= K214), R244 (= R236)
binding adenosine-2'-5'-diphosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), A71 (= A73), T75 (≠ R77), G160 (= G162), M161 (≠ A163)

3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 3pwkA

query
sites
3pwkA

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

A
|
A

G
|
G

A

M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), A71 (= A73), T75 (≠ R77), G160 (= G162)
binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R100), N126 (= N128), G158 (= G160), A159 (= A161), E219 (= E211), K222 (= K214), R244 (= R236)

2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 2gz3A

query
sites
2gz3A

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K

K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N
|
N

L

L

R

L

K

K

G

G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

active site: C127 (= C129), Q154 (= Q156), R244 (= R236), H251 (= H243)
binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C129), Q154 (= Q156), G158 (= G160), E219 (= E211), R244 (= R236), H251 (= H243)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ R77), N93 (= N95), G158 (= G160), G160 (= G162), M161 (≠ A163), N324 (= N316), A329 (= A321)

2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 2gz2A

query
sites
2gz2A

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A

A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

A

A

G

G

A

M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E

E

H

M

K

K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

active site: C127 (= C129), Q154 (= Q156), R244 (= R236), H251 (= H243)
binding adenosine-2'-5'-diphosphate: G8 (= G10), T10 (= T12), G11 (= G13), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), A71 (= A73), T75 (≠ R77)

2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/357 of 2gz1A

query
sites
2gz1A

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S
|
S

G
|
G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E

E

H

M

K

K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N
|
N

L
|
L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

active site: C127 (= C129), Q154 (= Q156), R244 (= R236), H251 (= H243)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ R77), N93 (= N95), S157 (= S159), G158 (= G160), G160 (= G162), M161 (≠ A163), N324 (= N316), L325 (= L317)

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/359 of 4r5hA

query
sites
4r5hA

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G75), T94 (≠ S96), S95 (= S97), R98 (= R100), N126 (= N128), C127 (= C129), Q154 (= Q156), G158 (= G160), K222 (= K214), R244 (= R236), H251 (= H243)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ R77), N93 (= N95), T94 (≠ S96), P125 (= P127), N126 (= N128), C127 (= C129), G160 (= G162), M161 (≠ A163), G328 (= G320)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/359 of 4r4jA

query
sites
4r4jA

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H
|
H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S96), S95 (= S97), R98 (= R100), N126 (= N128), C127 (= C129), Q154 (= Q156), G158 (= G160), E219 (= E211), K222 (= K214), R244 (= R236), H251 (= H243)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ R77), N93 (= N95), T94 (≠ S96), N126 (= N128), C127 (= C129), G160 (= G162), M161 (≠ A163), G328 (= G320)

3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/359 of 3pyxB

query
sites
3pyxB

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S
|
S

G
|
G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N
|
N

L
|
L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R100), G158 (= G160), E219 (= E211), K222 (= K214), R244 (= R236)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ R77), C127 (= C129), S157 (= S159), G158 (= G160), G160 (= G162), M161 (≠ A163), N324 (= N316), L325 (= L317)

3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/358 of 3q11A

query
sites
3q11A

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A

A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

D

S

R
x
T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

A
x
M

Q
x
G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A35 (= A37), S36 (= S38), R38 (= R40), S39 (= S41), T56 (≠ L58), A71 (= A73), T75 (≠ R77), G160 (= G162), M161 (≠ A163), G162 (≠ Q164)
binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R100), N126 (= N128), C127 (= C129), Q154 (= Q156), G158 (= G160), E219 (= E211), K222 (= K214), R244 (= R236)

4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/360 of 4r51A

query
sites
4r51A

G

G

Y

Y

T

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A
|
A

S
|
S

A

A

R

R

S
|
S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L
x
T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

D

S

R

T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

A

A

G
|
G

A
x
M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E

E

H

M

K
|
K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (= T12), G11 (= G13), A12 (= A14), V13 (= V15), A35 (= A37), S36 (= S38), S39 (= S41), T56 (≠ L58), S70 (= S72), A71 (= A73), G72 (= G74), N93 (= N95), T94 (≠ S96), N126 (= N128), C127 (= C129), G160 (= G162), M161 (≠ A163), G328 (= G320)
binding phthalic acid: S73 (≠ G75), T94 (≠ S96), S95 (= S97), R98 (= R100), N126 (= N128), K222 (= K214)

3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
53% identity, 99% coverage: 3:338/338 of query aligns to 1:346/361 of 3pylC

query
sites
3pylC

G

G

Y

Y

T

T

V

V

A

A

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

Q

A

E

Q

M

M

L

I

K

K

I

M

L

L

E

E

R

S

D

T

F

L

P

P

V

I

A

D

K

K

L

I

K

R

A

Y

L

L

A

A

S

S

A

A

R

R

S

S

A

A

G

G

K

K

K

S

V

L

K

K

F

F

K

K

G

D

E

Q

E

D

V

I

T

T

V

I

E

E

L

T

R

T

P

E

E

T

S

A

F

F

E

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

D

S

R

T

S

S

R

A

Q

K

F

Y

A

A

P

P

E

Y

A

A

V

V

K

K

R

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

A

V

L

E

D

W

A

H

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

L

K

A

W

K

G

I

L

K

D

R

R

V

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

A

A

G

G

A

M

Q

G

A

A

I

I

E

L

E

E

L

T

K

Q

E

R

Q

E

T

L

K

R

A

E

V

V

L

L

E

N

G

D

R

G

P

V

V

K

P

P

P

C

-

D

-

L

-

H

-

A

-

E

-

I

-

L

-

P

-

S

-

G

A

G

N

D

K

K

I

K

P

H

R

Y

Q

P

I

I

A

A

F

F

N

N

C

A

V

L

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

Y

E
|
E

E

E

H

M

K

K

M

M

I

T

N

K

E

E

T

T

K

K

I

I

M

M

H

E

D

D

S

S

I

I

K

A

V

V

S

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

V

S

G

A

H

H

S

S

E

E

V

S

V

V

N

Y

I

I

E

E

F

T

E

K

K

E

P

V

I

A

T

P

V

I

E

E

Q

E

A

V

R

K

E

A

A

A

L

I

E

A

R

A

A

F

P

P

G

G

V

A

E

V

V

L

V

E

D

D

F

V

K

A

N

H

H

Q

I

I

Y

Y

P

P

T

Q

P

A

I

I

D

N

V

A

A

V

G

G

K

S

D

R

P

D

V

T

L

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

D

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

G

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

E

E

K

R

G

G

L

L

V

V

binding 3-amino-D-alanine: N126 (= N128), C127 (= C129), G158 (= G160), E219 (= E211), R244 (= R236)

8jusA Crystal structure of aspartate semialdehyde dehydrogenase from porphyromonas gingivalis complexed with 2',5'adenosine diphosphate
54% identity, 98% coverage: 6:336/338 of query aligns to 3:333/335 of 8jusA

query
sites
8jusA

V

I

A

A

V

I

V

V

G
|
G

A

V

T
x
S

G
|
G

A

A

V

V

G

G

Q

Q

E

E

M

F

L

L

K

R

I

V

L

L

E

S

E

Q

R

R

D

D

F

F

P

P

V

I

A

D

K

G

L

L

K

Y

A

L

L

F

A
x
G

S
|
S

A

S

R
|
R

S
|
S

A

A

G

G

K

S

K

V

V

Y

K

S

F

F

K

K

G

G

E

K

E

E

V

Y

T

V

V

V

E

R

E

E

L

L

R

K

P

D

-

N

E

D

S

D

F

F

E

K

G

G

V

I

D

D

I

I

A

A

L

F

F

C

S
|
S

A
|
A

G

G

D

D

R
x
T

S

S

R

I

Q

A

F

F

A

A

P

D

E

T

A

I

V

T

K

R

R

H

G

G

A

T

V

L

V

M

I

I

D

D

N

N

S

S

A

A

F

F

R

R

M

M

E

Q

P

E

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

G

E

D

D

D

V

A

E

L

W

V

H

H

-

P

K

R

G

N

I

I

A

S

N

N

P

P

N

N

C

C

S

T

T

T

I

I

Q

Q

M

M

V

V

V

A

L

L

K

K

P

P

L

I

H

E

D

D

L

L

A

S

K

H

I

I

K

R

R

R

V

V

V

H

V

V

A

A

T

T

Y

Y

Q

Q

A

A

V

A

S

S

G

G

A

A

G

G

A

A

Q

L

A

G

I

M

E

A

E

E

L

L

K

V

E

Q

Q

Q

T

V

K

E

A

E

V

L

L

A

E

R

G

G

R

E

P

K

V

-

P

P

P

T

A

V

N

D

K

K

I

F

P

A

R

Y

Q

Q

I

L

A

M

F

Y

N

N

C

L

V

I

P

P

H

Q

I

I

D

D

K

V

F

F

F

T

D

D

D

N

G

D

Y

Y

T

T

R

K

E

E

H

M

K

K

M

M

I

Y

N

R

E

E

T

T

K

K

K

R

I

I

M

M

H

H

D

S

D

D

S

-

I

V

K

M

V

V

S

S

P

A

T

T

C

C

V

V

R

R

V

V

P

P

V

V

F

M

V

R

G

A

H

H

S

S

E

E

V

A

V

I

N

W

I

V

E

E

F

T

E

E

K

R

P

P

I

I

T

A

V

P

E

E

Q

E

A

A

R

R

E

A

A

A

L

F

E

A

R

K

A

A

P

P

G

G

V

V

E

L

V

L

V

C

D

D

D

E

F

P

K

S

N

E

H

K

I

Q

Y

Y

P

P

T

M

P

P

I

L

D

F

V

G

A

T

G

E

K

Q

D

D

P

P

V

V

L

I

V

V

G

G

R

R

I

I

R

R

R

Q

D

D

D

L

T

A

I

N

E

P

N

N

G

G

L

L

N

V

L

F

W

W

I

C

V

V

G

S

D

D

N

Q

L

I

R

R

K

K

G

G

A

A

L

L

N

N

A

A

V

V

Q

Q

I

I

A

A

E

E

L

Y

L

L

V

I

E

A

K

K

G

G

binding adenosine-2'-5'-diphosphate: G7 (= G10), S9 (≠ T12), G10 (= G13), G34 (≠ A37), S35 (= S38), R37 (= R40), S38 (= S41), S70 (= S72), A71 (= A73), T75 (≠ R77)

3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
50% identity, 98% coverage: 3:333/338 of query aligns to 1:341/342 of 3tz6A

query
sites
3tz6A

G

G

Y

L

T

S

V

I

A

G

V

I

V

V

G

G

A

A

T

T

G

G

A

Q

V

V

G

G

Q

Q

E

V

M

M

L

R

K

T

I

L

L

L

E

D

E

E

R

R

D

D

F

F

P

P

V

A

A

S

K

A

L

V

K

R

A

F

L

F

A

A

S

S

A

A

R

R

S

S

A

Q

G

G

K

R

K

K

V

L

K

A

F

F

K

R

G

G

E

Q

E

E

V

I

T

E

V

V

E

E

E

D

L

A

R

E

P

T

E

A

S

D

F

P

E

S

G

G

V

L

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

D

A

R

M

S

S

R

K

Q

V

F

Q

A

A

P

P

E

R

A

F

V

A

K

A

R

A

G

G

A

V

V

T

V

V

I

I

D

D

N

N

S

S

A

A

F

W

R
|
R

M

K

E

D

P

P

D

D

V

V

P

P

L

L

V

V

P
x
S

E

E

V

V

N

N

P

F

E

E

D

R

V

-

E

D

W

A

H
|
H

-

R

-
x
R

-

P

K

K

G

G

I

I

A

A

N

N

P

P

N
|
N

C
|
C

S

T

T

T

I

M

Q

A

M

A

V

M

V

P

V

V

L

L

K

K

P

V

L

L

H

H

D

D

L

E

A

A

K

R

I

L

K

V

R

R

V

L

V

V

A

S

T

S

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

S

G

G

A

L

Q

A

A

G

I

V

E

A

E

E

L

L

K

A

E

E

Q

Q

T

A

K

R

A

A

V

V

L

I

E

G

G

G

R

A

-

E

-

Q

-

L

-

V

-

Y

-

D

-
x
G

-

G

-

A

-

L

P

E

V
x
F

P

P

A

P

N

N

K

T

I

Y

P

V

R

A

Q
x
P

I

I

A

A

F

F

N

N

C

V

V

V

P

P

H

L

I

A

D

G

K

S

F

L

F

V

D

D

G

G

Y

S

-

G

-

E

T

T

R

D

E
|
E

E

D

H
x
Q

K
|
K

M

L

I
x
R

N
x
F

E

E

T

S

K

R

K

K

I

I

M

L

H

G

D

I

D

P

S

D

I

L

K

L

V

V

S

S

P

G

T

T

C

C

V

V

R
|
R

V

V

P

P

V

V

F

F

V

T

G

G

H
|
H

S

S

E

L

V

S

V

I

N

N

I

A

E

E

F

F

E

A

K

Q

P

P

I

L

T

S

V

P

E
|
E

Q

R

A

A

R
|
R

E

E

A

L

L

L

E

D

R

G

A

A

P

T

G

G

V

V

E

Q

V

L

V

V

D

D

D

-

F

-

K

-

N

-

H

-

I

-

Y

V

P

P

T

T

P

P

I

L

D

A

V

A

A

A

G

G

K

V

D

D

P

E

V

S

L

L

V

V

G

G

R

R

I

I

R

R

D

D

D

P

T

G

I

V

E

P

N

D

-

G

-

R

G

G

L

L

N

A

L

L

W

F

I

V

V

S

G

G

D

D

N

N

L

L

R

R

K

K

G

G

A

A

L

L

N

N

A

T

V

I

Q
|
Q

I

I

A

A

E
|
E

L
|
L

L

L

V

T

active site: C129 (= C129), Q156 (= Q156), R248 (= R236), H255 (= H243)
binding cysteine: C129 (= C129), Q156 (= Q156), G160 (= G160), E223 (= E211), R248 (= R236), H255 (= H243)
binding glycerol: S108 (≠ P110), G187 (vs. gap), F192 (≠ V182), P201 (≠ Q191), Q225 (≠ H213), R228 (≠ I216), F229 (≠ N217), Q335 (= Q327), E338 (= E330), L339 (= L331)
binding sulfate ion: R98 (= R100), H117 (= H120), R119 (vs. gap), N128 (= N128), C129 (= C129), K226 (= K214), E270 (= E258), R273 (= R261)

Query Sequence

>WP_013537108.1 NCBI__GCF_000185805.1:WP_013537108.1
MKGYTVAVVGATGAVGQEMLKILEERDFPVAKLKALASARSAGKKVKFKGEEVTVEELRP
ESFEGVDIALFSAGGDRSRQFAPEAVKRGAVVIDNSSAFRMEPDVPLVVPEVNPEDVEWH
KGIIANPNCSTIQMVVVLKPLHDLAKIKRVVVATYQAVSGAGAQAIEELKEQTKAVLEGR
PVPPANKIPRQIAFNCVPHIDKFFDDGYTREEHKMINETKKIMHDDSIKVSPTCVRVPVF
VGHSEVVNIEFEKPITVEQAREALERAPGVEVVDDFKNHIYPTPIDVAGKDPVLVGRIRR
DDTIENGLNLWIVGDNLRKGAALNAVQIAELLVEKGLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory