SitesBLAST
Comparing WP_013537260.1 NCBI__GCF_000185805.1:WP_013537260.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
44% identity, 96% coverage: 1:467/484 of query aligns to 1:464/485 of Q8DLI5
- R6 (= R6) binding L-glutamate
- Y192 (= Y195) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
44% identity, 96% coverage: 2:467/484 of query aligns to 1:463/484 of 2cfoA
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
44% identity, 100% coverage: 3:484/484 of query aligns to 103:563/564 of 3al0C
- active site: S110 (= S10), K335 (= K254)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R6), A108 (= A8), P109 (= P9), G118 (= G18), T122 (= T22), E142 (= E42), Y276 (= Y195), R294 (= R213), G295 (= G214), D297 (= D216), H298 (= H217), L324 (≠ M243), I325 (= I244), L333 (= L252)
- binding : T144 (= T44), D145 (= D45), R148 (= R48), Y208 (≠ C108), P213 (≠ M116), K252 (= K170), M255 (≠ I173), I266 (≠ V185), K269 (≠ R188), S270 (= S189), Y276 (= Y195), D297 (= D216), H298 (= H217), L299 (≠ I218), S300 (= S219), N301 (= N220), K304 (= K223), R330 (= R249), P332 (≠ K251), G363 (= G282), W364 (= W283), R365 (≠ Y284), E370 (= E290), S387 (≠ N307), K389 (≠ A309), V391 (≠ A311), I392 (≠ V312), K397 (= K317), W400 (= W320), R407 (= R327), E446 (= E366), K447 (≠ Y367), Q453 (≠ D373), I457 (≠ Y377), R509 (≠ K430), K520 (= K441), Q524 (≠ M445), R527 (= R448), V535 (≠ S456), T536 (≠ G457), G538 (≠ E459), L539 (= L460)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
40% identity, 99% coverage: 1:480/484 of query aligns to 1:461/471 of P04805
- C98 (= C106) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C108) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C133) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (= H135) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ T137) mutation to Q: No change in activity or in zinc content.
- H131 (≠ A139) mutation to Q: No change in activity or in zinc content.
- H132 (≠ E140) mutation to Q: No change in activity or in zinc content.
- C138 (≠ P151) mutation to S: No change in activity or in zinc content.
- S239 (= S253) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
40% identity, 99% coverage: 1:480/484 of query aligns to 1:461/468 of 8i9iA
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of 2cv2A
- active site: K246 (= K254)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R6), A7 (= A8), S9 (= S10), G17 (= G18), I21 (≠ T22), E41 (= E42), Y187 (= Y195), R205 (= R213), A206 (≠ G214), E208 (≠ D216), W209 (≠ H217), L235 (≠ M243), L236 (≠ I244)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V152), R163 (≠ K170), Y168 (≠ I175), E172 (≠ Q179), V177 (= V185), K180 (≠ R188), S181 (= S189), Y187 (= Y195), E207 (= E215), E208 (≠ D216), W209 (≠ H217), V211 (≠ S219), R237 (≠ L245), K241 (≠ R249), L272 (= L280), M273 (≠ L281), G274 (= G282), E282 (= E290), S299 (≠ N307), P303 (≠ A311), V304 (= V312), K309 (= K317), W312 (= W320), R319 (= R327), P357 (≠ E366), R358 (≠ Y367), R417 (≠ K430), Q432 (≠ M445), R435 (= R448), L442 (≠ T455), E443 (≠ S456), T444 (≠ G457), G446 (≠ E459), L447 (= L460), F448 (≠ P461)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of 2cv1A
- active site: K246 (= K254)
- binding adenosine-5'-triphosphate: P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ N19), I21 (≠ T22), R47 (= R48), A206 (≠ G214), W209 (≠ H217), L235 (≠ M243), L236 (≠ I244)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R6), A7 (= A8), E41 (= E42), Y187 (= Y195), R205 (= R213), W209 (≠ H217)
- binding : S9 (= S10), E41 (= E42), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V152), R163 (≠ K170), V166 (≠ I173), E172 (≠ Q179), V177 (= V185), K180 (≠ R188), S181 (= S189), Y187 (= Y195), E207 (= E215), E208 (≠ D216), W209 (≠ H217), V211 (≠ S219), R237 (≠ L245), K241 (≠ R249), K243 (= K251), M273 (≠ L281), G274 (= G282), S276 (≠ Y284), E282 (= E290), S299 (≠ N307), P303 (≠ A311), V304 (= V312), K309 (= K317), W312 (= W320), R319 (= R327), P357 (≠ E366), R358 (≠ Y367), R417 (≠ K430), L427 (≠ G440), Q432 (≠ M445), R435 (= R448), L442 (≠ T455), E443 (≠ S456), T444 (≠ G457), G446 (≠ E459), L447 (= L460), F448 (≠ P461)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of 1n78A
- active site: K246 (= K254)
- binding glutamol-amp: R5 (= R6), A7 (= A8), P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ N19), I21 (≠ T22), E41 (= E42), Y187 (= Y195), N191 (≠ V199), R205 (= R213), A206 (≠ G214), E208 (≠ D216), W209 (≠ H217), L235 (≠ M243), L236 (≠ I244)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V152), R163 (≠ K170), V166 (≠ I173), Y168 (≠ I175), E172 (≠ Q179), V177 (= V185), K180 (≠ R188), S181 (= S189), Y187 (= Y195), E207 (= E215), E208 (≠ D216), W209 (≠ H217), L210 (≠ I218), V211 (≠ S219), R237 (≠ L245), K241 (≠ R249), M273 (≠ L281), G274 (= G282), E282 (= E290), R297 (≠ D305), P303 (≠ A311), V304 (= V312), K309 (= K317), W312 (= W320), R319 (= R327), P357 (≠ E366), R358 (≠ Y367), R417 (≠ K430), L427 (≠ G440), Q432 (≠ M445), R435 (= R448), L442 (≠ T455), E443 (≠ S456), T444 (≠ G457), G446 (≠ E459), L447 (= L460), F448 (≠ P461)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of 1j09A
- active site: K246 (= K254)
- binding adenosine-5'-triphosphate: H15 (= H16), E208 (≠ D216), L235 (≠ M243), L236 (≠ I244), K243 (= K251), I244 (≠ L252), S245 (= S253), K246 (= K254), R247 (= R255)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (= E42), Y187 (= Y195), N191 (≠ V199), R205 (= R213), W209 (≠ H217)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of P27000
- R358 (≠ Y367) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
39% identity, 98% coverage: 2:477/484 of query aligns to 1:476/485 of 4griB
- active site: S9 (= S10), K253 (= K254)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (= E42), Y194 (= Y195), R212 (= R213), W216 (≠ H217)
- binding zinc ion: C105 (= C106), C107 (= C108), Y128 (= Y129), C132 (= C133)
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
41% identity, 99% coverage: 3:481/484 of query aligns to 2:468/468 of 1g59A
- binding : D44 (= D45), R45 (≠ T46), A46 (≠ E47), R47 (= R48), P109 (≠ Q110), V145 (= V152), R163 (≠ K170), V166 (≠ I173), E172 (≠ Q179), V177 (= V185), K180 (≠ R188), S181 (= S189), D182 (≠ N190), E207 (= E215), E208 (≠ D216), R237 (≠ L245), K241 (≠ R249), T242 (≠ S250), K243 (= K251), M273 (≠ L281), G274 (= G282), E282 (= E290), S299 (≠ N307), L300 (≠ S308), P303 (≠ A311), V304 (= V312), K309 (= K317), W312 (= W320), R319 (= R327), P357 (≠ E366), R358 (≠ Y367), R417 (≠ K430), K426 (= K439), L427 (≠ G440), Q432 (≠ M445), R435 (= R448), L442 (≠ T455), E443 (≠ S456), T444 (≠ G457), P445 (≠ V458), G446 (≠ E459), L447 (= L460), F448 (≠ P461)
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
39% identity, 100% coverage: 3:484/484 of query aligns to 3:502/502 of 6brlA
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
38% identity, 100% coverage: 2:483/484 of query aligns to 3:469/488 of 8vc5A
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
46% identity, 73% coverage: 3:354/484 of query aligns to 3:324/380 of 4g6zA
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
31% identity, 61% coverage: 6:299/484 of query aligns to 19:282/308 of P27305
- E55 (= E42) binding L-glutamate
- Y182 (= Y195) binding L-glutamate
- R200 (= R213) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
34% identity, 52% coverage: 6:255/484 of query aligns to 7:230/290 of 4a91A
- active site: S11 (= S10), K229 (= K254)
- binding glutamic acid: R7 (= R6), A9 (= A8), S11 (= S10), E43 (= E42), Y170 (= Y195), R188 (= R213), L192 (≠ H217)
- binding zinc ion: C99 (= C106), C101 (= C108), Y113 (= Y129), C117 (= C133)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
36% identity, 49% coverage: 3:240/484 of query aligns to 11:228/455 of 3aiiA
Sites not aligning to the query:
P47897 Glutamine--tRNA ligase; Glutaminyl-tRNA synthetase; GlnRS; EC 6.1.1.18 from Homo sapiens (Human) (see 2 papers)
25% identity, 72% coverage: 3:352/484 of query aligns to 264:608/775 of P47897
- R403 (= R154) to W: in MSCCA; results in loss of glutaminyl-tRNA aminoacylation activity; impairs protein folding; does not interact with RARS1; results in reduced protein solubility; dbSNP:rs587777332
- R515 (vs. gap) to W: in MSCCA; results in loss of glutaminyl-tRNA aminoacylation activity; impairs protein folding; results in reduced protein solubility; dbSNP:rs587777334
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 45 G → V: in MSCCA; results in reduced glutaminyl-tRNA aminoacylation activity; does not affect interaction with RARS1; dbSNP:rs587777331
- 57 Y → H: in MSCCA; results in reduced glutaminyl-tRNA aminoacylation activity; does not affect interaction with RARS1; dbSNP:rs587777333
- 175 H→A: Decreases catalytic efficiency about 60-fold.
Query Sequence
>WP_013537260.1 NCBI__GCF_000185805.1:WP_013537260.1
MSVRVRFAPSPTGFMHVGNARTALFNYLFARHNNGTFILRIEDTDTERHSEEAVKVIYEA
LKWMGLDWDEGPDRGGPYGPYRQSERLNIYKEFIEKLKEKGLVYECYCTQEELEQMRKEQ
LERGEPPRYTGKCRHLTEAEKEKLRAEGRKPVLRFKVPEDRVVKFKDLVKGEIEINSRQL
GGDFVIVRSNGMPVYNFVVVVDDALMKITHVIRGEDHISNTPKQLLLYEALGFEAPQFAH
LPMILGSDRSKLSKRHGSTSVKEFREKGYLPEAFTNFLALLGWYPKDGKEILSMEELIER
FDIKDVNSAPAVFDTTKLNWMNQVYIRNYPIEKLTELVIPYLEKAGFNLSEFDKQWLQKV
VEVTREYFTVLADAPEYMEVFLKDQFEVEPEAKKFIEESPDRLRVIELFYEKVKELQEEL
TSETFKKLVKEIGKELGVKGKNLFMPVRIGLTGRTSGVELPVLVELLGKERVLRRLENSL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory