SitesBLAST
Comparing WP_013553338.1 NCBI__GCF_000186245.1:WP_013553338.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 10 hits to proteins with known functional sites (download)
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
45% identity, 67% coverage: 18:202/277 of query aligns to 11:197/304 of Q96XT4
- C12 (= C19) binding [4Fe-4S] cluster
- C15 (= C22) binding [4Fe-4S] cluster
- IGCS 44:47 (= IGCS 51:54) binding thiamine diphosphate
- C46 (= C53) binding [4Fe-4S] cluster
- D90 (= D95) binding Mg(2+)
- GD 91:92 (= GD 96:97) binding thiamine diphosphate
- N118 (= N123) binding Mg(2+)
- V120 (≠ I125) binding Mg(2+)
- GL 122:123 (= GL 127:128) binding thiamine diphosphate
- C197 (= C202) binding [4Fe-4S] cluster
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
45% identity, 67% coverage: 18:202/277 of query aligns to 8:194/301 of 5b46B
- binding magnesium ion: D87 (= D95), N115 (= N123), V117 (≠ I125)
- binding iron/sulfur cluster: W8 (= W18), C9 (= C19), C12 (= C22), C43 (= C53), C194 (= C202)
- binding thiamine diphosphate: I41 (= I51), G42 (= G52), C43 (= C53), S44 (= S54), H62 (= H70), G86 (= G94), G88 (= G96), D89 (= D97), N115 (= N123), V117 (≠ I125), Y118 (= Y126), G119 (= G127), L120 (= L128), T121 (= T129)
Sites not aligning to the query:
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
36% identity, 86% coverage: 13:251/277 of query aligns to 19:257/291 of 6n2oD
- binding magnesium ion: D101 (= D95), N129 (= N123), I131 (= I125)
- binding succinyl-coenzyme a: I57 (= I51), R62 (= R56), L134 (= L128), K136 (≠ N130)
- binding iron/sulfur cluster: W24 (= W18), C25 (= C19), C28 (= C22), C59 (= C53), C208 (= C202), T210 (≠ I204), F211 (≠ N205)
- binding thiamine diphosphate: I57 (= I51), G58 (= G52), C59 (= C53), S60 (= S54), H76 (= H70), G102 (= G96), D103 (= D97), N129 (= N123), I131 (= I125), G133 (= G127), L134 (= L128), T135 (= T129)
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
36% identity, 86% coverage: 13:251/277 of query aligns to 19:257/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (= R56), L134 (= L128)
- binding coenzyme a: K136 (≠ N130), Y150 (≠ V144)
- binding magnesium ion: D101 (= D95), N129 (= N123), I131 (= I125)
- binding iron/sulfur cluster: W24 (= W18), C25 (= C19), C28 (= C22), C59 (= C53), C208 (= C202), T210 (≠ I204), F211 (≠ N205)
- binding thiamine diphosphate: I57 (= I51), G58 (= G52), C59 (= C53), S60 (= S54), H76 (= H70), G102 (= G96), D103 (= D97), N129 (= N123), I131 (= I125), Y132 (= Y126), G133 (= G127), L134 (= L128), T135 (= T129)
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
36% identity, 86% coverage: 13:251/277 of query aligns to 19:257/291 of 6n2nB
- binding magnesium ion: D101 (= D95), N129 (= N123), I131 (= I125)
- binding iron/sulfur cluster: W24 (= W18), C25 (= C19), C28 (= C22), H30 (≠ D24), C59 (= C53), C208 (= C202), T210 (≠ I204), F211 (≠ N205)
- binding thiamine diphosphate: I57 (= I51), G58 (= G52), C59 (= C53), S60 (= S54), H76 (= H70), G100 (= G94), D101 (= D95), G102 (= G96), D103 (= D97), N129 (= N123), I131 (= I125), Y132 (= Y126), G133 (= G127), L134 (= L128), T135 (= T129)
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
44% identity, 67% coverage: 18:202/277 of query aligns to 4:179/275 of 5b47B
- binding magnesium ion: D83 (= D95), N111 (= N123)
- binding iron/sulfur cluster: C5 (= C19), C8 (= C22), C39 (= C53), C179 (= C202)
- binding thiamine diphosphate: I37 (= I51), G38 (= G52), C39 (= C53), S40 (= S54), H58 (= H70), G84 (= G96), D85 (= D97), N111 (= N123), V113 (≠ I125), Y114 (= Y126), L116 (= L128)
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
35% identity, 92% coverage: 18:273/277 of query aligns to 11:279/305 of P72579
- K49 (≠ R56) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (= L128) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
35% identity, 92% coverage: 18:273/277 of query aligns to 11:279/305 of Q96Y68
- C12 (= C19) binding [4Fe-4S] cluster
- C15 (= C22) binding [4Fe-4S] cluster
- C46 (= C53) binding [4Fe-4S] cluster
- K49 (≠ R56) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D95) binding Mg(2+)
- GD 91:92 (= GD 96:97) binding thiamine diphosphate
- N118 (= N123) binding Mg(2+)
- V120 (≠ I125) binding Mg(2+)
- GL 122:123 (= GL 127:128) binding thiamine diphosphate
- K125 (≠ N130) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (≠ R178) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C202) binding [4Fe-4S] cluster
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
37% identity, 92% coverage: 18:273/277 of query aligns to 7:261/286 of 5b48B
- binding magnesium ion: D84 (= D95), N112 (= N123), V114 (≠ I125)
- binding iron/sulfur cluster: C8 (= C19), C11 (= C22), C42 (= C53), C183 (= C202), T185 (vs. gap)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I51), G41 (= G52), C42 (= C53), S43 (= S54), H59 (= H70), G85 (= G96), D86 (= D97), N112 (= N123), V114 (≠ I125), Y115 (= Y126), G116 (= G127), L117 (= L128)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
32% identity, 28% coverage: 69:145/277 of query aligns to 402:477/539 of 6lpiB
- active site: M403 (≠ H70), D428 (= D95), N455 (= N123), A457 (≠ I125), L458 (≠ Y126), L460 (= L128), V461 (≠ T129), Q464 (= Q132)
- binding magnesium ion: D428 (= D95), N455 (= N123)
- binding thiamine diphosphate: M403 (≠ H70), G427 (= G94), D428 (= D95), G429 (= G96), S430 (≠ D97), M433 (≠ I101), N455 (= N123), A457 (≠ I125), L458 (≠ Y126), G459 (= G127), L460 (= L128), V461 (≠ T129)
Sites not aligning to the query:
- active site: 27, 29, 30, 31, 32, 53, 76, 115, 116, 117, 165, 256, 283, 375, 401
- binding flavin-adenine dinucleotide: 155, 212, 213, 214, 236, 237, 238, 254, 256, 257, 276, 277, 278, 280, 282, 283, 300, 301, 319, 320, 380, 398
- binding thiamine diphosphate: 53, 76, 79, 376, 377, 378, 401
Query Sequence
>WP_013553338.1 NCBI__GCF_000186245.1:WP_013553338.1
MAFNYDEYLRTDKMPTLWCWGCGDGVILKAFIRAIDKLGIKKDDMCIVSGIGCSGRFSSY
IDCNTVHTTHGRTIAYATGIKMANPDKHVFCITGDGDGLAIGGNHTIHGCRRNIDINHIL
IDNFIYGLTNSQTSPTTPQGMWTVTMKRGNIDPTFDACKLADAAGASFVAREKVSEPRKL
EKVFVEAFKHKGYSFVDVFSNCHINLGRKNKMANAMENLDWIDSITVSKKKYDAMSEEER
KGLFPTGVLKKDEEAREYTEMYEKVKEAWQNNTKVEL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory