SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013553443.1 NCBI__GCF_000186245.1:WP_013553443.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
45% identity, 99% coverage: 5:560/564 of query aligns to 99:663/670 of P17597

query
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P17597

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A122 (= A28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E50) binding thiamine diphosphate
S186 (= S92) binding FAD
P197 (= P103) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ S105) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q113) binding thiamine diphosphate
K220 (= K126) binding (R)-imazaquin
R246 (= R152) binding (R)-imazaquin; binding FAD
K256 (= K162) binding chlorimuron-ethyl
G308 (= G212) binding FAD
TL 331:332 (= TL 237:238) binding FAD
C340 (≠ A246) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 255:258) binding FAD
GVRFD 371:375 (≠ GARFD 277:281) binding FAD
DR 376:377 (= DR 282:283) binding chlorimuron-ethyl
DI 395:396 (= DI 301:302) binding FAD
DV 414:415 (= DV 320:321) binding FAD
QH 487:488 (= QH 391:392) binding thiamine diphosphate
GG 508:509 (= GG 412:413) binding FAD
GAM 511:513 (≠ GTM 415:417) binding thiamine diphosphate
D538 (= D442) binding Mg(2+)
DGS 538:540 (= DGS 442:444) binding thiamine diphosphate
N565 (= N469) binding Mg(2+)
NQHLGM 565:570 (≠ NHFLGM 469:474) binding thiamine diphosphate
H567 (≠ F471) binding Mg(2+)
W574 (= W478) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A550) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/585 of 5k2oA

query
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5k2oA

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E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M257), R292 (= R283), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), G286 (= G277), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), Q404 (= Q393), M405 (= M394), G423 (= G412)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), M428 (= M417), D453 (= D442), G454 (= G443), S455 (= S444), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/583 of 5k3sA

query
sites
5k3sA

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
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Y

P

P

G
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G

A
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A

I
x
S

M

M

N

E

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I

Y

H

D

Q

E

A

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K

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N

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R

H

H

E
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E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

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R

A

S

T

S

G

G

E

K

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P

G

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A

C

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T
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T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

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V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

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D

S

S

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P

P

L

L

V

V

V

A

I

I

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T

G

G

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Q

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P

P

L

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S

R

L

M

I

I

G

G

T

T

D

D

G

A

F
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F

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Q

E
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E

V

T

D

P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

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Y

L

L

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R

M

S

D

I

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E

E

D

D

L

I

P

P

R

R

I

I

L

I

K

E

E

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

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R
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R

P

P

G

G

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P

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V

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L

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V

D

D

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P

K
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K

D

D

I

I

T

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G

Q

E

Q

M

L

G

A

E

I

F

P

I

N

Y

W

P

E

D

Q

H

A

V

M

N

R

L

L

P

P

T

G

Y

Y

K

M

P

S

R

R

Y

M

K

P

G

K

-

P

N

E

D

D

R

S

Q

H

I

L

K

E

K

Q

A

I

V

V

E

R

A

L

I

I

V

S

K

E

A

S

K

K

P

P

L

V

L

L

Y

Y

I

V

G
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G

T

C

V

L

L

-

S

-

N

N

A

S

Y

S

Q

D

E

E

V

L

R

G

E

R

F

F

A

V

E

E

L

L

T

T

Q

G

I

I

P

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T

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V

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T

L
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L

M
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M

A

G

R

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G

G

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G

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A

C

K

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N

D

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L

G

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M

H

H

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G

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Y

V

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Y

A

A

N

N

M

Y

A

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S

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E

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T

S

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D

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R

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I

I

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D

I
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I

D

D

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A

A

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I

I

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G

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N

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D

H

Y

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P

S

I

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C

G

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D
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D

V
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V

K

K

T

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V

A

V

L

-

Q

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-

M

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N

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-

Y

F

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R

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D

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E

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A

I

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Q

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Y

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A

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I

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R

V

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S

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A

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V

G
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H
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H

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Q

M
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M

W

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T

A

A

A

Q

Q

F

F

Y

Y

P

N

F

Y

D

K

R

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P

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R

Q

Q

W

W

I

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N

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S

S

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G

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M

G

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G

G

F

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P

P

A

A

I

I

G

G

A

A

K

S

V

V

G

A

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N

P

P

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D

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A

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I

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V

I

V

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D

F

I

T

D

G

G

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D

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S

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F

L

I

M

M

N

N

V

V

Q

Q

E

E

L

L

V

A

T

T

A

I

A

R

E

V

Y

E

G

N

V

L

P

P

V

V

I

K

N

V

I

L

L

L

N

N

N
|
N

H

Q

F
x
H

L
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L

G
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G

M
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M

V
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V

R

M

Q

Q

W
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W

Q

E

T

D

F

R

F

F

Y

Y

D

K

K

A

R

N

Y

R

S

A

E

H

T

T

D

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

S

E

Y

I

Q

F

P

P

D

N

W

M

K

L

L

F

A

A

E

A

A

A

C

C

G

G

G

I

A

P

G

A

Y

A

D

R

V

V

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T

T

K

K

K

A

A

E

D

F

L

D

R

E

E

A

A

L

I

R

Q

S

T

A

M

M

L

E

D

G

T

G

P

K

G

V

P

T

Y

F

L

I

L

N

D

V

V

A

I

V

C

D

P

R
x
H

F

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A

S

G
|
G

G

G

A

T

L

F

Y

N

N

D

M

V

L

I

L

T

E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R283), M485 (= M474), W489 (= W478), G569 (= G551)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), M266 (= M257), G286 (= G277), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), G426 (= G415), M428 (= M417), D453 (= D442), G454 (= G443), S455 (= S444), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 8et4A

query
sites
8et4A

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

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V

K

E

T

T

V

V

F

F

G

A

Y

Y

P

P

G

G

A

A

I

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M

N

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Y

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E

A

I

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S

A

S

F

I

K

R

H

N

I

V

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L

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R

R

H

H

E

E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

E

K

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P

G

G

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I

A

C

M

I

V

A

T

T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

P

P

L

R

S

R

L

M

I

I

G

G

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T

D

D

G

A

F

F

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Q

E

E

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A

I

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V

G

E

I

V

S

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R

R

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T

T

K

K

H

H

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Y

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L

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E

D

D

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I

I

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E

E

E

A

A

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A

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R

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Y

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-

P

N

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A

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A

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P

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L

L

Y

Y

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G

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C

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L

L

-

S

-

N

N

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D

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R

F

F

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M

A

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L

G

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M

H
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H

G

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Y

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A

Y

A

A

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N

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G

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x
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R

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D

D

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T

G

G

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I
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I

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A

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I

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G

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D

V
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V

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-

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F

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H
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H

Q

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M
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M

W

W

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A

A

A

Q

Q

F

F

Y

Y

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Y

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L

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x
H

L
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L

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M
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M

V
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R

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Q

Q

W
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W

Q

E

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F

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F

F

Y

Y

D

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K

A

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N

Y

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A

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H

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F

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L

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A

A

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A

A

A

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C

G

G

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K

A

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H

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V

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L

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P

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M

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A
x
S

G

G

A

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D

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E

E

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), G426 (= G415), M428 (= M417), G452 (= G441), D453 (= D442), G454 (= G443), S455 (= S444), M458 (= M447), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412)
binding magnesium ion: F370 (≠ Y359), D453 (= D442), M458 (= M447), Q461 (= Q450), N480 (= N469), H482 (≠ F471), K533 (= K515)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M257), R292 (= R283), M485 (= M474), W489 (= W478), S568 (≠ A550)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 5wj1A

query
sites
5wj1A

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (= L255), G286 (= G277), R288 (= R279), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M257), D291 (= D282), R292 (= R283), M485 (= M474), W489 (= W478), S568 (≠ A550)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), M428 (= M417), D453 (= D442), G454 (= G443), S455 (= S444), M458 (= M447), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 5k6tA

query
sites
5k6tA

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E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H258), R292 (= R283), M485 (= M474), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), G286 (= G277), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), Q404 (= Q393), M405 (= M394), G423 (= G412)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), G426 (= G415), M428 (= M417), G452 (= G441), G454 (= G443), S455 (= S444), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 5k6rA

query
sites
5k6rA

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

L

Y

T

K

R

Q

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N

S

A

S

F

I

K

R

H

N

I

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L

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P

R

R

H

H

E
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E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

E

K

V

P

G

G

V

I

A

C

M

I

V

A

T
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T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

I

I

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T

G

G

Q

Q

V

V

P

P

L

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S

R

L

M

I

I

G

G

T

T

D

D

G

A

F
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F

Q
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Q

E
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E

V

T

D

P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

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Y

L

L

V

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R

M

S

D

I

V

E

E

D

D

L

I

P

P

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R

I

I

L

I

K

E

E

E

A

A

F

F

Y

F

I

L

A

A

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S

S

G

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R
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R

P

P

G

G

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P

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V

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L

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V

D

D

I

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K

D

D

I

I

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G

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Q

M

L

G

A

E

I

F

P

I

N

Y

W

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D

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N

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L

L

P

P

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G

Y

Y

K

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K

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G

K

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N

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D

D

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L

K

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K

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A

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I

I

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K

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A

S

K

K

P

P

L

V

L

L

Y

Y

I

V

G
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G

T

C

V

L

L

-

S

-

N

N

A

S

Y

S

Q

D

E

E

V

L

R

G

E

R

F

F

A

V

E

E

L

L

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T

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G

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I

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T

L
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L

M
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M

A

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N

D

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L

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L

G

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M

H

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G

G

S

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Y

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A

Y

A

A

N

N

M

Y

A

A

M

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E

E

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D

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L

I

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G

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R

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V

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G

G

K

K

L

L

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E

E

A

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A

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I

I

I

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I

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D

I
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I

D

D

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A

A

N

E

I

I

G

G

K

K

L

N

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H

Y

V

P

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I

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V

C

G
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G

D
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D

V
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V

K

K

T

L

V

A

V

L

-

Q

-

G

-

M

N

N

K

K

M

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L

E

E

M

N

I

R

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A

E

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K

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L

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Y

F

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V

V

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W

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N

I

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L

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N

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K

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D

G

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A

I

I

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Q

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Y

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A

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I

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K

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G

D

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L

L

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D

S

G

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K

A

A

I

I

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S

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G

V
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V

G
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G

Q
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Q

H
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H

Q

Q

M
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M

W

W

T

A

A

A

Q

Q

F

F

Y

Y

P

N

F

Y

D

K

R

K

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P

R

R

Q

Q

W

W

I

L

N

S

S

S

G
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G

G

G

L

L

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G

T

A

M
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M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

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S

V

V

G

A

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N

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D

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A

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I

V

V

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V

N

D

F

I

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D

G

G

D
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D

G
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G

S
|
S

I

F

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I

M
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M

N

N

V

V

Q

Q

E

E

L

L

V

A

T

T

A

I

A

R

E

V

Y

E

G

N

V

L

P

P

V

V

I

K

N

V

I

L

L

L

N

N

N
|
N

H

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
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W

Q

E

T

D

F

R

F

F

Y

Y

D

K

K

A

R

N

Y

R

S

A

E

H

T

T

D

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

S

E

Y

I

Q

F

P

P

D

N

W

M

K

L

L

F

A

A

E

A

A

A

C

C

G

G

G

I

A

P

G

A

Y

A

D

R

V

V

T

T

T

K

K

K

A

A

E

D

F

L

D

R

E

E

A

A

L

I

R

Q

S

T

A

M

M

L

E

D

G

T

G

P

K

G

V

P

T

Y

F

L

I

L

N

D

V

V

A

I

V

C

D

P

R
x
H

F

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

T

L

F

Y

N

N

D

M

V

L

I

L

T

E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R283), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), M266 (= M257), G286 (= G277), R288 (= R279), R292 (= R283), V293 (= V284), D310 (= D301), I311 (= I302), G328 (= G319), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), G426 (= G415), M428 (= M417), D453 (= D442), G454 (= G443), S455 (= S444), M458 (= M447), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 1z8nA

query
sites
1z8nA

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
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Y

P

P

G
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G

A
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A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

L

Y

T

K

R

Q

S

N

S

A

S

F

I

K

R

H

N

I

V

L

L

T

P

R

R

H

H

E
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E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

E

K

V

P

G

G

V

I

A

C

M

I

V

A

T
|
T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

P

P

L

R

S

R

L

M

I

I

G

G

T

T

D

D

G

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F
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F

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Q

E
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E

V

T

D

P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

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K
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K

H

H

N

N

Y

Y

L

L

V

V

R

M

S

D

I

V

E

E

D

D

L

I

P

P

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R

I

I

L

I

K

E

E

E

A

A

F

F

Y

F

I

L

A

A

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S

S

G

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R
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R

P

P

G

G

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P

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L

L

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V

D

D

I

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K

D

D

I

I

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Y

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R

Y

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N

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K

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P

L

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L

L

Y

Y

I

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G
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G

G

G

G
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G

T

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V

L

L

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S

-

N

N

A

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M

A

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L

G
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G

M
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H
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H

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N

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V
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V

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G

G

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D

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L

L

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N

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N

H

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x
H

L
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L

G
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G

M
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M

V
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V

R

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Q

W
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W

Q

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F

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K

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W

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C

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K

K

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M

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H

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H

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L

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M

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G
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G

G

G

A

T

L

F

Y

N

N

D

M

V

L

I

L

T

E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K126), R161 (= R152), Y191 (= Y182), R194 (= R185), D291 (= D282), R292 (= R283), D312 (= D303), W489 (= W478), G569 (= G551)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469)
binding thiamine diphosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), G426 (= G415), M428 (= M417), G452 (= G441), G454 (= G443), S455 (= S444), N480 (= N469), H482 (≠ F471), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 1yi1A

query
sites
1yi1A

G

G

A

A

Q

D

M

I

V

L

C

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E

E

A

A

I

L

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A

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E

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T

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V

F

F

G

A

Y
|
Y

P

P

G
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G

A
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A

I
x
S

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M

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E

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I

Y

H

D

Q

E

A

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R

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H

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E
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E

Q

Q

A

G

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H

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A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

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S

G

G

E

K

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P

G

G

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I

A

C

M

I

V

A

T
|
T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

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P

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L

L

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A

I

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G

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Q

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I

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E

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I

V

V

G

E

I

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S

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R

R

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S

C

I

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K

K

H

H

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I

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E

D

D

L

I

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I

I

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E

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A

A

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I

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R

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K

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L

L

Y

Y

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G

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G

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N

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L

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G

M
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H

G

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Y

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A

Y

A

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M

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A

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E

H

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D

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x
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I

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I

I

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D

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M

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M

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G

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A

I

A

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Y

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V

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L

L

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N

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|
N

H

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x
H

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|
L

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G

M
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M

V
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V

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M

Q

Q

W
|
W

Q

E

T

D

F

R

F

F

Y

Y

D

K

K

A

R

N

Y

R

S

A

E

H

T

T

D

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

S

E

Y

I

Q

F

P

P

D

N

W

M

K

L

L

F

A

A

E

A

A

A

C

C

G

G

G

I

A

P

G

A

Y

A

D

R

V

V

T

T

T

K

K

K

A

A

E

D

F

L

D

R

E

E

A

A

L

I

R

Q

S

T

A

M

M

L

E

D

G

T

G

P

K

G

V

P

T

Y

F

L

I

L

N

D

V

V

A

I

V

C

D

P

R
x
H

F

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

T

L

F

Y

N

N

D

M

V

L

I

L

T

E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D282), R292 (= R283), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (= L255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 1yi0A

query
sites
1yi0A

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

L

Y

T

K

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S

N

S

A

S

F

I

K

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H

N

I

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L

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T

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R

R

H

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E
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E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

E

K

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P

G

G

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I

A

C

M

I

V

A

T
|
T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

I

I

S

T

G

G

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Q

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V

P

P

L

R

S

R

L

M

I

I

G

G

T

T

D

D

G

A

F
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F

Q
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Q

E
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E

V

T

D

P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

Y

Y

L

L

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V

R

M

S

D

I

V

E

E

D

D

L

I

P

P

R

R

I

I

L

I

K

E

E

E

A

A

F

F

Y

F

I

L

A

A

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S

S

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|
R

P

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G

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K

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G

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H

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G

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E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D282), R292 (= R283), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D301), I311 (= I302), G328 (= G319), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 1yhzA

query
sites
1yhzA

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

L

Y

T

K

R

Q

S

N

S

A

S

F

I

K

R

H

N

I

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

E

K

V

P

G

G

V

I

A

C

M

I

V

A

T
|
T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

P

P

L

R

S

R

L

M

I

I

G

G

T

T

D

D

G

A

F
|
F

Q
|
Q

E
|
E

V

T

D

P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

Y

Y

L

L

V

V

R

M

S

D

I

V

E

E

D

D

L

I

P

P

R

R

I

I

L

I

K

E

E

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

G

Q

E

Q

M

L

G

A

E

I

F

P

I

N

Y

W

P

E

D

Q

H

A

V

M

N

R

L

L

P

P

T

G

Y

Y

K

M

P

S

R

R

Y

M

K

P

G

K

-

P

N

E

D

D

R

S

Q

H

I

L

K

E

K

Q

A

I

V

V

E

R

A

L

I

I

V

S

K

E

A

S

K

K

P

P

L

V

L

L

Y

Y

I

V

G

G

G
|
G

T

C

V

L

L

-

S

-

N

N

A

S

Y

S

Q

D

E

E

V

L

R

G

E

R

F

F

A

V

E

E

L

L

T

T

Q

G

I

I

P

P

T

V

V

A

E

S

T
|
T

L
|
L

M
|
M

A

G

R

L

G

G

V

S

M

Y

G

P

A

C

K

D

N

D

P

E

L

L

L

S

V

L

G

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

S

T

Y

V

A

Y

A

A

N

N

M

Y

A

A

M

V

S

E

E

H

T

S

D

D

L

L

I

L

S

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

S

E

E

A

F

F

A

A

R

S

Y

R

A

A

D

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

N

E

I

I

G

G

K

K

L

N

V

K

D

T

V

P

D

H

Y

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

T

L

V

A

V

L

-

Q

-

G

-

M

N

N

K

K

M

V

L

L

E

E

M

N

I

R

R

A

E

E

K

E

V

L

D

K

P

L

D

D

R

-

Y

F

Q

G

V

V

W

W

R

R

D

N

I

E

L

L

A

N

R

V

Y

Q

D

K

E

Q

L

K

H

F

P

P

L

L

S

S

Y

F

T

K

D

T

S

F

D

G

E

E

V

A

I

I

K

P

P

P

Q

Q

W

Y

V

A

V

I

E

K

R

V

V

L

G

D

E

E

L

L

V

T

G

D

S

G

D

K

A

A

I

I

T

S

S

T

D

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

T

A

A

A

Q

Q

F

F

Y

Y

P

N

F

Y

D

K

R

K

P

P

R

R

Q

Q

W

W

I

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

V

V

G

A

V

N

P

P

E

D

K

A

T

I

V

V

I

V

N

D

F

I

T

D

G

G

D
|
D

G

G

S

S

I

F

L

I

M

M

N

N

V

V

Q

Q

E

E

L

L

V

A

T

T

A

I

A

R

E

V

Y

E

G

N

V

L

P

P

V

V

I

K

N

V

I

L

L

L

N

N

N
|
N

H

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

F

R

F

F

Y

Y

D

K

K

A

R

N

Y

R

S

A

E

H

T

T

D

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

S

E

Y

I

Q

F

P

P

D

N

W

M

K

L

L

F

A

A

E

A

A

A

C

C

G

G

G

I

A

P

G

A

Y

A

D

R

V

V

T

T

T

K

K

K

A

A

E

D

F

L

D

R

E

E

A

A

L

I

R

Q

S

T

A

M

M

L

E

D

G

T

G

P

K

G

V

P

T

Y

F

L

I

L

N

D

V

V

A

I

V

C

D

P

R
x
H

F

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

T

L

F

Y

N

N

D

M

V

L

I

L

T

E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D282), R292 (= R283), M485 (= M474), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), Q404 (= Q393), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 1yhyA

query
sites
1yhyA

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

L

Y

T

K

R

Q

S

N

S

A

S

F

I

K

R

H

N

I

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

E

K

V

P

G

G

V

I

A

C

M

I

V

A

T
|
T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

P

P

L

R

S

R

L

M

I

I

G

G

T

T

D

D

G

A

F
|
F

Q
|
Q

E
|
E

V

T

D

P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

Y

Y

L

L

V

V

R

M

S

D

I

V

E

E

D

D

L

I

P

P

R

R

I

I

L

I

K

E

E

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

G

Q

E

Q

M

L

G

A

E

I

F

P

I

N

Y

W

P

E

D

Q

H

A

V

M

N

R

L

L

P

P

T

G

Y

Y

K

M

P

S

R

R

Y

M

K

P

G

K

-

P

N

E

D

D

R

S

Q

H

I

L

K

E

K

Q

A

I

V

V

E

R

A

L

I

I

V

S

K

E

A

S

K

K

P

P

L

V

L

L

Y

Y

I

V

G
|
G

T

C

V

L

L

-

S

-

N

N

A

S

Y

S

Q

D

E

E

V

L

R

G

E

R

F

F

A

V

E

E

L

L

T

T

Q

G

I

I

P

P

T

V

V

A

E

S

T
|
T

L
|
L

M
|
M

A

G

R

L

G

G

V

S

M

Y

G

P

A

C

K

D

N

D

P

E

L

L

L

S

V

L

G

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

S

T

Y

V

A

Y

A

A

N

N

M

Y

A

A

M

V

S

E

E

H

T

S

D

D

L

L

I

L

S

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

S

E

E

A

F

F

A

A

R

S

Y

R

A

A

D

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

N

E

I

I

G

G

K

K

L

N

V

K

D

T

V

P

D

H

Y

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

T

L

V

A

V

L

-

Q

-

G

-

M

N

N

K

K

M

V

L

L

E

E

M

N

I

R

R

A

E

E

K

E

V

L

D

K

P

L

D

D

R

-

Y

F

Q

G

V

V

W

W

R

R

D

N

I

E

L

L

A

N

R

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Y

Q

D

K

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Q

L

K

H

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P

P

L

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Y

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D

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A

I

I

K

P

P

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Q

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Y

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A

V

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V

L

G

D

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L

L

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T

G

D

S

G

D

K

A

A

I

I

T

S

S

T

D

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

T

A

A

A

Q

Q

F

F

Y

Y

P

N

F

Y

D

K

R

K

P

P

R

R

Q

Q

W

W

I

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

V

V

G

A

V

N

P

P

E

D

K

A

T

I

V

V

I

V

N

D

F

I

T

D

G

G

D
|
D

G

G

S

S

I

F

L

I

M

M

N

N

V

V

Q

Q

E

E

L

L

V

A

T

T

A

I

A

R

E

V

Y

E

G

N

V

L

P

P

V

V

I

K

N

V

I

L

L

L

N

N

N
|
N

H

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

F

R

F

F

Y

Y

D

K

K

A

R

N

Y

R

S

A

E

H

T

T

D

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

S

E

Y

I

Q

F

P

P

D

N

W

M

K

L

L

F

A

A

E

A

A

A

C

C

G

G

G

I

A

P

G

A

Y

A

D

R

V

V

T

T

T

K

K

K

A

A

E

D

F

L

D

R

E

E

A

A

L

I

R

Q

S

T

A

M

M

L

E

D

G

T

G

P

K

G

V

P

T

Y

F

L

I

L

N

D

V

V

A

I

V

C

D

P

R
x
H

F

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

T

L

F

Y

N

N

D

M

V

L

I

L

T

E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D282), R292 (= R283), V486 (= V475), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), Q404 (= Q393), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 1ybhA

query
sites
1ybhA

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

V

V

K

E

T

T

V

V

F

F

G

A

Y
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Y

P

P

G
|
G

A
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A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

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Y

T

K

R

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N

S

A

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F

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K

R

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N

I

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R

R

H

H

E
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E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

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R

A

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S

G

G

E

K

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P

G

G

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A

C

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T
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T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

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T

S

G

G

L

L

A

A

T

D

A

A

Y

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M

L

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D

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S

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A

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Q

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M

I

I

G

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T

D

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A

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F

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E
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E

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P

A

I

V

V

G

E

I

V

S

T

R

R

P

S

C

I

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T

K

K

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H

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N

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Y

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I

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E

E

D

D

L

I

P

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I

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K

E

E

E

A

A

F

F

Y

F

I

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A

A

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S

G

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R
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R

P

P

G

G

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P

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L

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V

D

D

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K

D

D

I

I

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Q

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L

G

A

E

I

F

P

I

N

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W

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L

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N

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I

I

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S

K

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A

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K

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P

L

V

L

L

Y

Y

I

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G

G

G
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G

T

C

V

L

L

-

S

-

N

N

A

S

Y

S

Q

D

E

E

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E

R

F

F

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E

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T

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M

A

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Y

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N

D

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L

G

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M

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H

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Y

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A

Y

A

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D

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G

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x
V

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R

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V

T

T

G

G

K

K

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E

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R

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A

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I

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D

I
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I

D

D

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A

A

N

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I

I

G

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D

H

Y

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S

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C

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D

V
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V

K

K

T

L

V

A

V

L

-

Q

-

G

-

M

N

N

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K

M

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M

N

I

R

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A

E

E

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L

D

K

P

L

D

D

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Y

F

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W

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N

I

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D

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E

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A

I

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Y

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A

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V

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D

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E

L

L

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D

S

G

D

K

A

A

I

I

T

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S

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D

G

V
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V

G

G

Q

Q

H

H

Q
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Q

M
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M

W

W

T

A

A

A

Q

Q

F

F

Y

Y

P

N

F

Y

D

K

R

K

P

P

R

R

Q

Q

W

W

I

L

N

S

S

S

G
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G

L

L

G
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G

T

A

M
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M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

V

V

G

A

V

N

P

P

E

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K

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I

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F

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M

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N

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Q

E

E

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T

A

I

A

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E

V

Y

E

G

N

V

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P

P

V

V

I

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N

V

I

L

L

L

N

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N
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N

H

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x
H

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L

G

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M
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M

V
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V

R

M

Q

Q

W
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W

Q

E

T

D

F

R

F

F

Y

Y

D

K

K

A

R

N

Y

R

S

A

E

H

T

T

D

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

S

E

Y

I

Q

F

P

P

D

N

W

M

K

L

L

F

A

A

E

A

A

A

C

C

G

G

G

I

A

P

G

A

Y

A

D

R

V

V

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T

T

K

K

K

A

A

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D

F

L

D

R

E

E

A

A

L

I

R

Q

S

T

A

M

M

L

E

D

G

T

G

P

K

G

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P

T

Y

F

L

I

L

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D

V

V

A

I

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C

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P

R
x
H

F

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

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T

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N

N

D

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V

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I

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E

E

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V389), G426 (= G415), M428 (= M417), D453 (= D442), N480 (= N469), H482 (≠ F471), L483 (= L472), M485 (= M474), V486 (= V475), W489 (= W478), H558 (≠ R540)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M257), D291 (= D282), R292 (= R283), M485 (= M474), W489 (= W478), S568 (≠ A550)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (= L255), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), Q404 (= Q393), M405 (= M394), G423 (= G412), G424 (= G413)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ F471)

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 13:577/582 of 3ea4A

query
sites
3ea4A

G

G

A

A

Q

D

M

I

V

L

C

V

E

E

A

A

I

L

I

E

A

R

E

Q

G

G

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V

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E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

N

E

V

I

Y

H

D

Q

E

A

I

L

Y

T

K

R

Q

S

N

S

A

S

F

I

K

R

H

N

I

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L

L

T

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R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

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S

G

G

E

K

V

P

G

G

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I

A

C

M

I

V

A

T
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T

S

S

G

G

P

P

G

G

F

A

T

T

N

N

A

L

V

V

T

S

G

G

L

L

A

A

T

D

A

A

Y

L

M

L

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D

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S

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P

P

L

L

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V

V

A

I

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G

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R

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I

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T

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F

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E

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G

E

I

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R

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S

C

I

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T

K

K

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H

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Y

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I

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E

E

D

D

L

I

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I

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E

E

E

A

A

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F

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F

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A

A

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S

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R
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R

P

P

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G

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L

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K

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P

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V

L

L

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Y

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G

T

C

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L

L

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-

N

N

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S

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M

A

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L

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G

M
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M

H
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H

G

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A

Y

A

A

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N

M

Y

A

A

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G

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R

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V

T

T

G

G

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I
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I

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V
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V

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G
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Q

H
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H

Q

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M
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M

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A

A

A

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F

F

Y

Y

P

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Y

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W

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M

G

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x
H

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L

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M

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F

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K

K

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L

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N

W

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L

F

A

A

E

A

A

A

C

C

G

G

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A

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G

A

Y

A

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K

K

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A

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F

L

D

R

E

E

A

A

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A

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M

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H

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H

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L

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M

M

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x
S

G

G

A

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M

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E

E

active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (= A28), S37 (≠ I29), E58 (= E50), T81 (= T73), F120 (= F112), Q121 (= Q113), E122 (= E114), K170 (= K162), M265 (= M257), V292 (= V284), V399 (= V389), G425 (= G415), M427 (= M417), D452 (= D442), N479 (= N469), H481 (≠ F471), L482 (= L472), M484 (= M474), V485 (= V475), W488 (= W478), H557 (≠ R540)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D282), R291 (= R283), W488 (= W478), S567 (≠ A550)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R152), G221 (= G211), G222 (= G212), G223 (= G213), T245 (= T237), L246 (= L238), M247 (= M239), L263 (= L255), G264 (= G256), M265 (= M257), H266 (= H258), G285 (= G277), R287 (= R279), D289 (= D281), R291 (= R283), D309 (= D301), I310 (= I302), G327 (= G319), D328 (= D320), V329 (= V321), M404 (= M394), G422 (= G412)
binding magnesium ion: D452 (= D442), N479 (= N469), H481 (≠ F471)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V389), G400 (= G390), Q401 (= Q391), H402 (= H392), M427 (= M417), G451 (= G441), D452 (= D442), G453 (= G443), S454 (= S444), N479 (= N469), H481 (≠ F471), L482 (= L472), G483 (= G473), M484 (= M474), V485 (= V475)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 13:577/582 of 3e9yA

query
sites
3e9yA

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active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (= A28), S37 (≠ I29), E58 (= E50), T81 (= T73), F120 (= F112), Q121 (= Q113), E122 (= E114), K170 (= K162), M265 (= M257), V292 (= V284), V399 (= V389), G425 (= G415), M427 (= M417), D452 (= D442), N479 (= N469), H481 (≠ F471), L482 (= L472), M484 (= M474), V485 (= V475), W488 (= W478), H557 (≠ R540)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D282), R291 (= R283), W488 (= W478), S567 (≠ A550)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R152), G221 (= G211), G222 (= G212), G223 (= G213), T245 (= T237), L246 (= L238), M247 (= M239), L263 (= L255), G285 (= G277), R287 (= R279), D289 (= D281), R291 (= R283), D309 (= D301), I310 (= I302), G327 (= G319), D328 (= D320), V329 (= V321), M404 (= M394), G422 (= G412)
binding magnesium ion: D452 (= D442), N479 (= N469), H481 (≠ F471)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V389), G400 (= G390), Q401 (= Q391), H402 (= H392), M427 (= M417), G451 (= G441), G453 (= G443), S454 (= S444), N479 (= N469), H481 (≠ F471), L482 (= L472), G483 (= G473), M484 (= M474), V485 (= V475)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
45% identity, 99% coverage: 5:560/564 of query aligns to 96:660/667 of P09342

query
sites
P09342

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C161 (≠ A70) modified: Disulfide link with 307
P194 (= P103) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ T214) modified: Disulfide link with 161

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 14:578/582 of 7tzzA

query
sites
7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M257), R292 (= R283), W489 (= W478), S568 (≠ A550)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V389), G401 (= G390), Q402 (= Q391), H403 (= H392), G426 (= G415), M428 (= M417), G452 (= G441), D453 (= D442), G454 (= G443), S455 (= S444), L483 (= L472), G484 (= G473), M485 (= M474), V486 (= V475)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (= L255), M266 (= M257), H267 (= H258), G286 (= G277), R288 (= R279), V293 (= V284), D310 (= D301), I311 (= I302), D329 (= D320), V330 (= V321), M405 (= M394), G423 (= G412)
binding magnesium ion: A37 (= A28), T82 (= T73), S83 (= S74), Q122 (= Q113), Y381 (≠ W370), D453 (= D442), M458 (= M447), Q461 (= Q450), N480 (= N469), H482 (≠ F471), K533 (= K515)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
45% identity, 99% coverage: 5:560/564 of query aligns to 93:657/664 of P09114

query
sites
P09114

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P191 (= P103) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W478) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
44% identity, 99% coverage: 3:559/564 of query aligns to 14:579/600 of 6derA

query
sites
6derA

M

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G

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I

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G

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x
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-

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-

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A

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L

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E

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x
N

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x
V

A

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G

G

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T

T

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A

Q

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K

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A

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E

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K

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E

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L

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M

M

V

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P

A
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A

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L

active site: Y35 (= Y24), G37 (= G26), G38 (= G27), A39 (= A28), I40 (= I29), E61 (= E50), T84 (= T73), F123 (= F112), Q124 (= Q113), E125 (= E114), K173 (= K162), K231 (≠ Q221), M267 (= M257), V294 (= V284), V410 (= V389), L435 (= L414), G436 (= G415), M438 (= M417), D463 (= D442), N490 (= N469), E492 (≠ F471), Q493 (≠ L472), M495 (= M474), V496 (= V475), W499 (= W478), L521 (= L501), N526 (≠ G506), V527 (≠ G507)
binding flavin-adenine dinucleotide: R163 (= R152), G220 (= G211), A221 (≠ G212), G222 (= G213), N225 (≠ S217), T247 (= T237), L248 (= L238), Q249 (≠ M239), L265 (= L255), H268 (= H258), G287 (= G277), A288 (= A278), R289 (= R279), D291 (= D281), R293 (= R283), V294 (= V284), E320 (≠ D301), I321 (= I302), N325 (= N306), G338 (= G319), D339 (= D320), V340 (= V321), Q414 (= Q393), M415 (= M394), G433 (= G412)
binding Metosulam: R293 (= R283), M495 (= M474), W499 (= W478), A570 (= A550)
binding magnesium ion: D463 (= D442), N490 (= N469), E492 (≠ F471)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (= V389), G411 (= G390), Q412 (= Q391), H413 (= H392), G436 (= G415), M438 (= M417), G462 (= G441), D463 (= D442), A464 (≠ G443), S465 (= S444), N490 (= N469), E492 (≠ F471), Q493 (≠ L472), G494 (= G473), M495 (= M474), V496 (= V475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (= V389), G411 (= G390), Q412 (= Q391), H413 (= H392), G436 (= G415), M438 (= M417), G462 (= G441), D463 (= D442), A464 (≠ G443), S465 (= S444), M468 (= M447), N490 (= N469), E492 (≠ F471), Q493 (≠ L472), G494 (= G473), V496 (= V475)

6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
44% identity, 99% coverage: 3:559/564 of query aligns to 12:577/598 of 6desA

query
sites
6desA

M

L

S

T

G

G

A

G

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F

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Y

P

A

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G

A
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I
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I

M

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N

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Y

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D

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A

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G

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A

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N

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G

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I

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A

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A

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Y

Y

I

A

G
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G

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x
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G

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-

V

I

L

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x
N

N

N

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-

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E

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L

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G

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A

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N

M

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M

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I

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L

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A

A

I

I

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A

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G

A

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V

I

I

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D

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G

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D

G
x
A

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S

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L

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M

M

N

T

V

L

Q

T

E

E

L

L

V

S

T

S

A

A

A

V

E

Q

Y

A

G

G

V

A

P

P

V

I

I

K

N

V

I

C

I

V

L

L

N

N

N
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N

H

E

F
x
E

L
x
Q

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G

M
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M

V
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V

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Q

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W

Q

Q

T

S

F

L

F

F

Y

Y

D

E

K

H

R

R

Y

Y

S

S

E

H

T

T

D

H

L

Q

S

S

Y

-

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N

P

P

D

D

W

F

K

M

K

K

L
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L

A

A

E

E

A

S

C

M

G
x
N

G
x
V

A

K

G

G

Y

I

D

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T

N

K

Q

A

Q

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E

F

L

D

K

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S

A

G

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V

R

K

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E

A

F

M

L

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G

A

G

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T

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F

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I

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N

E

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V

A

I

V

V

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E

R

K

F

K

E

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N

P

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V

L

L

P

P

M

M

V

V

P

P

A

A

G

G

G

K

A

A

L

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Y

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N

D

M

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L

I

L

L

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (= I29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), K229 (≠ Q221), M265 (= M257), V292 (= V284), V408 (= V389), L433 (= L414), G434 (= G415), M436 (= M417), D461 (= D442), N488 (= N469), E490 (≠ F471), Q491 (≠ L472), M493 (= M474), V494 (= V475), W497 (= W478), L519 (= L501), N524 (≠ G506), V525 (≠ G507)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (= M257), D290 (= D282), R291 (= R283), W497 (= W478)
binding flavin-adenine dinucleotide: R161 (= R152), G218 (= G211), A219 (≠ G212), G220 (= G213), N223 (≠ S217), T245 (= T237), L246 (= L238), Q247 (≠ M239), L263 (= L255), G285 (= G277), A286 (= A278), R287 (= R279), D289 (= D281), R291 (= R283), V292 (= V284), E318 (≠ D301), I319 (= I302), N323 (= N306), D337 (= D320), V338 (= V321), Q412 (= Q393), M413 (= M394), G431 (= G412)
binding magnesium ion: D461 (= D442), N488 (= N469), E490 (≠ F471)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (= V389), G409 (= G390), Q410 (= Q391), H411 (= H392), G434 (= G415), M436 (= M417), G460 (= G441), D461 (= D442), A462 (≠ G443), S463 (= S444), N488 (= N469), E490 (≠ F471), Q491 (≠ L472), G492 (= G473), M493 (= M474), V494 (= V475)

Query Sequence

>WP_013553443.1 NCBI__GCF_000186245.1:WP_013553443.1
MKMSGAQMVCEAIIAEGVKTVFGYPGGAIMNVYDEIYKQNAFKHILTRHEQAAVHAADGY
ARATGEVGVAMVTSGPGFTNAVTGLATAYMDSVPLVVISGQVPLSLIGTDGFQEVDAVGI
SRPCTKHNYLVRSIEDLPRILKEAFYIARSGRPGPVLVDIPKDITGEMGEFIYPDHVNLP
TYKPRYKGNDRQIKKAVEAIVKAKKPLLYIGGGTVLSNAYQEVREFAELTQIPTVETLMA
RGVMGAKNPLLVGMLGMHGSYAANMAMSETDLIISLGARFDDRVTGKLSEFARYADIIHV
DIDPANIGKLVDVDYPIVGDVKTVVNKMLEMIREKVDPDRYQVWRDILARYDELHPLSYT
DSDEVIKPQWVVERVGELVGSDAIITSDVGQHQMWTAQFYPFDRPRQWINSGGLGTMGYG
FPAAIGAKVGVPEKTVINFTGDGSILMNVQELVTAAEYGVPVINIILNNHFLGMVRQWQT
FFYDKRYSETDLSYQPDWKKLAEACGGAGYDVTTKAEFDEALRSAMEGGKVTFINVAVDR
FENVLPMVPAGGALYNMLLEHKDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory