SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013553508.1 NCBI__GCF_000186245.1:WP_013553508.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
38% identity, 93% coverage: 6:226/237 of query aligns to 2:223/232 of 1f3oA

query
sites
1f3oA

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binding adenosine-5'-diphosphate: Y11 (≠ F15), G41 (= G45), G43 (= G47), K44 (= K48), S45 (≠ T49), T46 (= T50)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
38% identity, 93% coverage: 6:226/237 of query aligns to 2:223/230 of 1l2tA

query
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1l2tA

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binding adenosine-5'-triphosphate: Y11 (≠ F15), S40 (= S44), G41 (= G45), S42 (≠ A46), G43 (= G47), K44 (= K48), S45 (≠ T49), T46 (= T50), F138 (vs. gap), Q145 (= Q148), S147 (= S150), G149 (= G152), Q150 (= Q153), H204 (= H207)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
40% identity, 92% coverage: 6:223/237 of query aligns to 4:217/226 of 5xu1B

query
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5xu1B

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binding magnesium ion: G45 (= G47), K46 (= K48), S47 (≠ T49)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
39% identity, 97% coverage: 6:234/237 of query aligns to 4:228/615 of 5lilA

query
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5lilA

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binding adenosine-5'-triphosphate: F13 (= F15), V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (≠ T49), T48 (= T50), Q92 (= Q97), K136 (= K141), Q143 (= Q148), S145 (= S150), G147 (= G152), Q148 (= Q153)
binding magnesium ion: S47 (≠ T49), Q92 (= Q97)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
39% identity, 97% coverage: 6:234/237 of query aligns to 4:228/592 of 5lj7A

query
sites
5lj7A

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binding adenosine-5'-triphosphate: V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (≠ T49), T48 (= T50), Q92 (= Q97), K136 (= K141), Q143 (= Q148), S145 (= S150), G147 (= G152), Q148 (= Q153)
binding magnesium ion: S47 (≠ T49), Q92 (= Q97)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
41% identity, 94% coverage: 6:227/237 of query aligns to 4:217/223 of 2pclA

query
sites
2pclA

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C

L

V

L

E

D

K

A

P

P

T

T

S

E

G

G

K

K

I

V

W

F

L

L

-

E

G

G

D

K

E

E

L

V

V

-

W

-

D

-

E

-

D

D

H

Y

W

T

S

N

I

E

R

K

D

E

P

L

R

S

K

L

I

L

R

R

R

N

E

R

K

K

L

L

G

G

F

F

I

V

F

F

Q

Q

A

F

H

H

Y

Y

L

L

I

I

P

P

F

E

L

L

N

T

I

A

L

L

E

E

N

N

V

V

I

I

L

V

I

P

P

M

T

L

T

K

N

M

G

G

I

K

P

P

R

K

K

K

A

E

A

A

E

K

K

E

K

R

A

G

M

E

E

Y

L

L

L

L

D

S

Y

E

F

L

D

G

I

L

G

G

D

D

K

K

A

L

H

S

A

R

M

K

P

P

S

Y

Q

E

L

L

S

S

G

G

G

G

Q

E

N

Q

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

A

N

N

D

E

P

P

H

I

I

L

V

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

A

G

A

N

L

L

D

D

M

S

S

A

R

N

A

T

V

K

N

R

V

V

V

M

K

D

M

I

L

F

R

L

K

K

I

I

A

-

K

N

E

E

R

G

Q

G

V

T

A

S

I

I

I

V

M

M

V

V

T
|
T

H

H

D

E

A

R

R

E

L

L

L

A

P

E

Y

L

C

T

D

H

K

R

I

T

L

L

S

E

I

M

E

K

N

D

R

G

K

K

V

V

V

V

binding magnesium ion: E165 (= E174), P166 (= P175), T167 (= T176), T196 (= T206)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
37% identity, 97% coverage: 1:229/237 of query aligns to 1:226/233 of P75957

query
sites
P75957

M

M

N

N

D

K

T

I

G

L

I

L

R

Q

V

C

E

D

N

N

L

L

T

C

K

K

I

R

F

Y

G

Q

K

E

G

G

D

S

A

V

Q

Q

V

T

R

D

V

V

L

L

E

H

N

N

A

V

S

S

L

F

E

S

V

V

K

G

K

E

G

G

E

E

F

M

A

M

A

A

L

I

V

V

A
x
G

P

S

S

S

G

G

A

S

G

G

K

K

T

S

T

T

L

L

L

L

M

H

M

L

I

L

G

G

C

G

V

L

E

D

K

T

P

P

T

T

S

S

G

G

K

D

I

V

W

I

L

F

G

N

D

G

E

Q

L

P

V

M

W

-

D

-

E

-

D

S

H

K

W

L

S

S

I

S

R

A

D

A

P

K

R

A

K

E

I

L

R

R

R

N

E

Q

K

K

L

L

G

G

F

F

I

I

F

Y

Q

Q

A

F

H

H

Y

H

L

L

I

L

P

P

F

D

L

F

N

T

I

A

L

L

E

E

N

N

V

V

I

A

L

M

I

-

P

P

T

L

T

L

N

I

G

G

I

-

P

K

R

K

K

K

A

P

A

A

E

E

-

I

-

N

K

S

K

R

A

A

M

L

E

E

L

M

L

L

D

K

Y

A

F

V

D

G

I

L

G

D

D

H

K

R

A

A

H

N

A

H

M

R

P

P

S

S

Q

E

L

L

S

S

G

G

G

G

Q

E

N

R

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

V

N

N

D

N

P

P

H

R

I

L

V

V

L

L

A

A

D

D

E

E

P

P

T

T

A

G

A

N

L

L

D

D

M

A

S

R

R

N

A

A

V

D

N

S

V

I

V

F

K

Q

M

L

L

L

R

G

K

E

I

L

A

N

K

R

E

L

R

Q

Q

G

V

T

A

A

I

F

I

L

M

V

V

V

T

T

H

H

D

D

A

L

R

Q

L

L

L

A

P

K

Y

R

C

M

D

S

K

R

I

Q

L

L

S

E

I

M

E

R

N

D

R

G

K

R

V

L

V

T

T

A

K

E

G42 (≠ A42) mutation to D: Loss of lipoprotein release when overexpressed.

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
36% identity, 94% coverage: 6:227/237 of query aligns to 5:222/648 of P75831

query
sites
P75831

I

L

R

E

V

L

E

K

N

D

L

I

T

R

K

R

I

S

F

Y

G

P

K

A

G

G

D

D

A

E

Q

Q

V

V

R

E

V

V

L

L

E

K

N

G

A

I

S

S

L

L

E

D

V

I

K

Y

K

A

G

G

E

E

F

M

A

V

A

A

L

I

V

V

A

G

P

A

S

S

G

G

A

S

G

G

K
|
K

T

S

T

T

L

L

L

M

M

N

M

I

I

L

G

G

C

C

V

L

E

D

K

K

P

A

T

T

S

S

G

G

K

T

I

Y

W

R

L

V

G

A

D

G

E

Q

L

D

V

V

W

A

D

T

E

L

D

D

H

A

W

D

S

A

I

L

R

A

D

-

P

-

R

-

K

Q

I

L

R

R

R

R

E

E

K

H

L

F

G

G

F

F

I

I

F

F

Q

Q

A

R

H

Y

Y

H

L

L

I

L

P

S

F

H

L

L

N

T

I

A

L

E

E

Q

N

N

V

V

I

E

L

V

I

P

P

A

T

V

T

Y

N

A

G

G

I

L

P

E

R

R

K

K

A

Q

A

R

E

L

K

L

K

R

A

A

M

Q

E

E

L

L

L

L

D

Q

Y

R

F

L

D

G

I

L

G

E

D

D

K

R

A

T

H

E

A

Y

M

Y

P

P

S

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

N

Q

Q

Q

R

R

A

V

A

S

I

I

A

A

R

R

A

A

L

L

S

M

N

N

D

G

P

G

H

Q

I

V

V

I

L

L

A

A

D
|
D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

M

S

S

H

R

S

A

G

V

E

N

E

V

V

V

M

K

A

M

I

L

L

R

H

K

Q

I

L

A

-

K

R

E

D

R

R

Q

G

V

H

A

T

I

V

I

I

M

I

V

V

T

T

H

H

D

D

A

P

R

Q

L

V

L

A

P

A

Y

Q

C

A

D

E

K

R

I

V

L

I

S

E

I

I

E

R

N

D

R

G

K

E

V

I

V

V

K47 (= K48) mutation to L: Lack of activity.
D169 (= D173) mutation to N: Lack of activity.

7mdyC Lolcde nucleotide-bound
37% identity, 95% coverage: 6:229/237 of query aligns to 3:223/226 of 7mdyC

query
sites
7mdyC

I

L

R

Q

V

C

E

D

N

N

L

L

T

C

K

K

I

R

F
x
Y

G

Q

K

E

G

G

D

S

A

V

Q

Q

V

T

R

D

V

V

L

L

E

H

N

N

A

V

S

S

L

F

E

S

V

V

K

G

K

E

G

G

E

E

F

M

A

M

A

A

L

I

V

V

A

G

P

S

S

S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

M

H

M

L

I

L

G

G

C

G

V

L

E

D

K

T

P

P

T

T

S

S

G

G

K

D

I

V

W

I

L

F

G

N

D

G

E

Q

L

P

V

M

W

-

D

-

E

-

D

S

H

K

W

L

S

S

I

S

R

A

D

A

P

K

R

A

K

E

I

L

R

R

R

N

E

Q

K

K

L

L

G

G

F

F

I

I

F

Y

Q
|
Q

A

F

H

H

Y

H

L

L

I

L

P

P

F

D

L

F

N

T

I

A

L

L

E

E

N

N

V

V

I

A

L

M

I

-

P

P

T

L

T

L

N

I

G

G

I

-

P

K

R

K

K

K

A

P

A

A

E

E

-

I

-

N

K

S

K

R

A

A

M

L

E

E

L

M

L

L

D

K

Y

A

F

V

D

G

I

L

G

D

D

H

K

R

A

A

H

N

A
x
H

M

R

P

P

S

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

N

R

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

V

N

N

D

N

P

P

H

R

I

L

V

V

L

L

A

A

D

D

E
|
E

P

P

T

T

A

G

A
x
N

L

L

D

D

M

A

S

R

R

N

A

A

V

D

N

S

V

I

V

F

K

Q

M

L

L

L

R

G

K

E

I

L

A

N

K

R

E

L

R

Q

Q

G

V

T

A

A

I

F

I

L

M

V

V

V

T

T

H
|
H

D

D

A

L

R

Q

L

L

L

A

P

K

Y

R

C

M

D

S

K

R

I

Q

L

L

S

E

I

M

E

R

N

D

R

G

K

R

V

L

V

T

T

A

K

E

binding adp orthovanadate: Y12 (≠ F15), G42 (= G45), S43 (≠ A46), G44 (= G47), K45 (= K48), S46 (≠ T49), T47 (= T50), Q91 (= Q97), H138 (≠ A144), E142 (≠ Q148), S144 (= S150), G145 (= G151), G146 (= G152), E168 (= E174), N172 (≠ A178), H201 (= H207)
binding magnesium ion: S46 (≠ T49), Q91 (= Q97)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
38% identity, 94% coverage: 6:227/237 of query aligns to 3:221/222 of 7arlD

query
sites
7arlD

I

L

R

Q

V

C

E

D

N

N

L

L

T

C

K

K

I

R

F

Y

G

Q

K

E

G

G

D

S

A

V

Q

Q

V

T

R

D

V

V

L

L

E

H

N

N

A

V

S

S

L

F

E

S

V

V

K

G

K

E

G

G

E

E

F

M

A

M

A

A

L

I

V

V

A

G

P

S

S

S

G
|
G

A

S

G
|
G

K
|
K

T
x
S

T

T

L

L

L

L

M

H

M

L

I

L

G

G

C

G

V

L

E

D

K

T

P

P

T

T

S

S

G

G

K

D

I

V

W

I

L

F

G

N

D

G

E

Q

L

P

V

M

W

-

D

-

E

-

D

S

H

K

W

L

S

S

I

S

R

A

D

A

P

K

R

A

K

E

I

L

R

R

R

N

E

Q

K

K

L

L

G

G

F

F

I

I

F

Y

Q

Q

A

F

H

H

Y

H

L

L

I

L

P

P

F

D

L

F

N

T

I

A

L

L

E

E

N

N

V

V

I

A

L

M

I

-

P

P

T

L

T

L

N

I

G

G

I

-

P

K

R

K

K

K

A

P

A

A

E

E

-

I

-

N

K

S

K

R

A

A

M

L

E

E

L

M

L

L

D

K

Y

A

F

V

D

G

I

L

G

D

D

H

K

R

A

A

H

N

A

H

M

R

P

P

S

S

Q

E

L

L

S

S

G

G

G

G

Q

E

N

R

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

V

N

N

D

N

P

P

H

R

I

L

V

V

L

L

A

A

D

D

E

E

P

P

T

T

A

G

A

N

L

L

D

D

M

A

S

R

R

N

A

A

V

D

N

S

V

I

V

F

K

Q

M

L

L

L

R

G

K

E

I

L

A

N

K

R

E

L

R

Q

Q

G

V

T

A

A

I

F

I

L

M

V

V

V

T

T

H

H

D

D

A

L

R

Q

L

L

L

A

P

K

Y

R

C

M

D

S

K

R

I

Q

L

L

S

E

I

M

E

R

N

D

R

G

K

R

V

L

V

T

binding adenosine-5'-diphosphate: G42 (= G45), G44 (= G47), K45 (= K48), S46 (≠ T49)

9gvkD Cryo-em structure of endogenous atp-bound lolcde with lold-e171q mutations in nanodiscs (see paper)
37% identity, 95% coverage: 6:229/237 of query aligns to 4:224/226 of 9gvkD

query
sites
9gvkD

I

L

R

Q

V

C

E

D

N

N

L

L

T

C

K

K

I

R

F
x
Y

G

Q

K

E

G

G

D

S

A

V

Q

Q

V

T

R

D

V

V

L

L

E

H

N

N

A

V

S

S

L

F

E

S

V

V

K

G

K

E

G

G

E

E

F

M

A

M

A

A

L

I

V

V

A

G

P

S

S
|
S

G
|
G

A

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

M

H

M

L

I

L

G

G

C

G

V

L

E

D

K

T

P

P

T

T

S

S

G

G

K

D

I

V

W

I

L

F

G

N

D

G

E

Q

L

P

V

M

W

-

D

-

E

-

D

S

H

K

W

L

S

S

I

S

R

A

D

A

P

K

R

A

K

E

I

L

R

R

R

N

E

Q

K

K

L

L

G

G

F

F

I

I

F

Y

Q
|
Q

A

F

H

H

Y

H

L

L

I

L

P

P

F

D

L

F

N

T

I

A

L

L

E

E

N

N

V

V

I

A

L

M

I

-

P

P

T

L

T

L

N

I

G

G

I

-

P

K

R

K

K

K

A

P

A

A

E

E

-

I

-

N

K

S

K

R

A

A

M

L

E

E

L

M

L

L

D

K

Y

A

F

V

D

G

I

L

G

D

D

H

K
x
R

A

A

H

N

A
x
H

M

R

P

P

S

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

N

R

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

V

N

N

D

N

P

P

H

R

I

L

V

V

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

A

N

L

L

D

D

M

A

S

R

R

N

A

A

V

D

N

S

V

I

V

F

K

Q

M

L

L

L

R

G

K

E

I

L

A

N

K

R

E

L

R

Q

Q

G

V

T

A

A

I

F

I

L

M

V

V

V

T

T

H

H

D

D

A

L

R

Q

L

L

L

A

P

K

Y

R

C

M

D

S

K

R

I

Q

L

L

S

E

I

M

E

R

N

D

R

G

K

R

V

L

V

T

T

A

K

E

binding adenosine-5'-triphosphate: Y13 (≠ F15), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (≠ T49), T48 (= T50), Q92 (= Q97), R136 (≠ K141), H139 (≠ A144), E143 (≠ Q148), S145 (= S150), G146 (= G151), G147 (= G152), E148 (≠ Q153)
binding magnesium ion: S47 (≠ T49), Q92 (= Q97)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
37% identity, 95% coverage: 6:229/237 of query aligns to 5:225/229 of 7v8iD

query
sites
7v8iD

I

L

R

Q

V

C

E

D

N

N

L

L

T

C

K

K

I

R

F

Y

G

Q

K

E

G

G

D

S

A

V

Q

Q

V

T

R

D

V
|
V

L

L

E

H

N

N

A

V

S

S

L

F

E

S

V

V

K

G

K

E

G

G

E

E

F

M

A

M

A

A

L

I

V

V

A

G

P

S

S
|
S

G
|
G

A

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

M

H

M

L

I

L

G

G

C

G

V

L

E

D

K

T

P

P

T

T

S

S

G

G

K

D

I

V

W

I

L

F

G

N

D

G

E

Q

L

P

V

M

W

-

D

-

E

-

D

S

H

K

W

L

S

S

I

S

R

A

D

A

P

K

R

A

K

E

I

L

R

R

R

N

E

Q

K

K

L

L

G

G

F

F

I

I

F

Y

Q
|
Q

A

F

H

H

Y

H

L

L

I

L

P

P

F

D

L

F

N

T

I

A

L

L

E

E

N

N

V

V

I

A

L

M

I

-

P

P

T

L

T

L

N

I

G

G

I

-

P

K

R

K

K

K

A

P

A

A

E

E

-

I

-

N

K

S

K

R

A

A

M

L

E

E

L

M

L

L

D

K

Y

A

F

V

D

G

I

L

G

D

D

H

K
x
R

A

A

H

N

A
x
H

M

R

P

P

S

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

N

R

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

V

N

N

D

N

P

P

H

R

I

L

V

V

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

A

N

L

L

D

D

M

A

S

R

R

N

A

A

V

D

N

S

V

I

V

F

K

Q

M

L

L

L

R

G

K

E

I

L

A

N

K

R

E

L

R

Q

Q

G

V

T

A

A

I

F

I

L

M

V

V

V

T

T

H
|
H

D

D

A

L

R

Q

L

L

L

A

P

K

Y

R

C

M

D

S

K

R

I

Q

L

L

S

E

I

M

E

R

N

D

R

G

K

R

V

L

V

T

T

A

K

E

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V24), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (≠ T49), T49 (= T50), Q93 (= Q97), R137 (≠ K141), H140 (≠ A144), E144 (≠ Q148), S146 (= S150), G148 (= G152), E149 (≠ Q153), H203 (= H207)
binding magnesium ion: S48 (≠ T49), Q93 (= Q97)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
35% identity, 94% coverage: 6:228/237 of query aligns to 5:223/650 of 5ws4A

query
sites
5ws4A

I

L

R

E

V

V

E

S

N

N

L

L

T

V

K

R

I

E

F

F

G

P

K

A

G

G

D

E

A

S

Q

T

V

I

R

Q

V

I

L

L

E

K

N

G

A

I

S

D

L

L

E

T

V

I

K

Y

K

E

G

G

E

E

F

L

A

V

A

A

L

I

V

V

A

G

P

Q

S
|
S

G
|
G

A

S

G

G

K

K

T
x
S

T

T

L

L

L

M

M

N

M

I

I

L

G

G

C

C

V

L

E

D

K

R

P

P

T

T

S

S

G

G

K

S

I

Y

W

K

L

V

G

N

D

G

E

Q

L

E

V

T

-

G

-

K

W

L

D

E

E

P

D

D

H

Q

W

L

S

A

I

-

R

-

D

-

P

-

R

-

K

Q

I

L

R

R

R

R

E

E

K

Y

L

F

G

G

F

F

I

I

F

F

Q

Q

A

R

H

Y

Y

H

L

L

I

L

P

G

F

D

L

L

N

S

I

A

L

E

E

G

N

N

V

V

I

E

L

V

I

P

P

A

T

V

T

Y

N

A

G

G

I

V

P

T

R

P

K

A

A

D

A

R

E

K

K

Q

K

R

A

A

M

T

E

A

L

L

L

L

D

T

Y

E

F

L

D

G

I

L

G

G

D

T

K

K

A

T

H

Q

A

N

M

R

P

P

S

S

Q

Q

L

L

S

S

G

G

G

G

Q

Q

N

Q

Q

Q

R

R

A

V

A

S

I

I

A

A

R

R

A

A

L

L

S

M

N

N

D

G

P

G

H

D

I

V

V

I

L

L

A

A

D

D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

M

S

S

H

R

S

A

G

V

V

N

E

V

V

V

M

K

R

M

I

L

L

R

R

K

E

I

L

A

N

K

A

E

A

R

G

Q

H

V

-

A

T

I

I

I

I

M

L

V

V

T

T

H

H

D

D

A

M

R

Q

L

V

L

A

P

K

Y

N

C

A

D

T

K

R

I

I

L

I

S

E

I

I

E

S

N

D

R

G

K

E

V

I

V

I

T

S

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S44), G44 (= G45), S48 (≠ T49)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 97% coverage: 6:236/237 of query aligns to 2:229/343 of P30750

query
sites
P30750

I

I

R

K

V

L

E

S

N

N

L

I

T

T

K

K

I

V

F

F

G

H

K

Q

G

G

D

T

A

R

Q

T

V

I

R

Q

V

A

L

L

E

N

N

N

A

V

S

S

L

L

E

H

V

V

K

P

K

A

G

G

E

Q

F

I

A

Y

A

G

L

V

V

I

A

G

P

A

S
|
S

G
|
G

A
|
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

M

R

M

C

I

V

G

N

C

L

V

L

E

E

K

R

P

P

T

T

S

E

G

G

K

S

I

V

W

L

L

V

-

D

G

G

D

Q

E

E

L

L

V

T

W

T

D

-

E

-

D

-

H

-

W

L

S

S

I

E

R

S

D

E

P

L

R

T

K

K

I

A

R

R

R

R

E

Q

K

-

L

I

G

G

F

M

I

I

F

F

Q

Q

A

H

H

F

Y

N

L

L

I

L

P

S

F

S

L

R

N

T

I

V

L

F

E

G

N

N

V

V

I

A

L

L

I

P

P

L

T

E

T

L

N

D

G

N

I

T

P

P

R

K

K

D

A

E

A

V

E

K

K

R

K

R

A

V

M

T

E

E

L

L

L

L

D

S

Y

L

F

V

D

G

I

L

G

G

D

D

K

K

A

H

H

D

A

S

M

Y

P

P

S

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

N

K

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

A

N

S

D

N

P

P

H

K

I

V

V

L

L

L

A

C

D

D

E
|
E

P

A

T

T

A

S

A

A

L

L

D

D

M

P

S

A

R

T

A

T

V

R

N

S

V

I

V

L

K

E

M

L

L

L

R

K

K

D

I

I

A

N

K

R

E

R

R

L

Q

G

V

L

A

T

I

I

I

L

M

L

V

I

T

T

H

H

D

E

A

M

R

D

L

V

L

V

P

K

-

R

Y

I

C

C

D

D

K

C

I

V

L

A

S

V

I

I

E

S

N

N

R

G

K

E

V

L

V

I

T

E

K

Q

T

D

Q

T

V

V

A

S

Q

E

E

V

F

F

40:46 (vs. 44:50, 86% identical) binding ATP
E166 (= E174) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
33% identity, 97% coverage: 6:236/237 of query aligns to 3:230/344 of 3tuzC

query
sites
3tuzC

I

I

R

K

V

L

E

S

N

N

L

I

T

T

K

K

I

V

F
|
F

G

H

K
x
Q

G

G

D

T

A

R

Q

T

V

I

R

Q

V

A

L

L

E

N

N

N

A

V

S

S

L

L

E

H

V

V

K

P

K

A

G

G

E

Q

F

I

A

Y

A

G

L

V

V

I

A

G

P

A

S

S

G
|
G

A

A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

M

R

M

C

I

V

G

N

C

L

V

L

E

E

K

R

P

P

T

T

S

E

G

G

K

S

I

V

W

L

L

V

-

D

G

G

D

Q

E

E

L

L

V

T

W

T

D

-

E

-

D

-

H

-

W

L

S

S

I

E

R

S

D

E

P

L

R

T

K

K

I

A

R

R

R

R

E

Q

K

-

L

I

G

G

F

M

I

I

F

F

Q

Q

A

H

H

F

Y

N

L

L

I

L

P

S

F

S

L

R

N

T

I

V

L

F

E

G

N

N

V

V

I

A

L

L

I

P

P

L

T

E

T

L

N

D

G

N

I

T

P

P

R

K

K

D

A

E

A

V

E

K

K

R

K

R

A

V

M

T

E

E

L

L

L

L

D

S

Y

L

F

V

D

G

I

L

G

G

D

D

K

K

A

H

H

D

A

S

M

Y

P

P

S

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

N

K

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

A

N

S

D

N

P

P

H

K

I

V

V

L

L

L

A

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

D

D

M

P

S

A

R

T

A

T

V

R

N

S

V

I

V

L

K

E

M

L

L

L

R

K

K

D

I

I

A

N

K

R

E

R

R

L

Q

G

V

L

A

T

I

I

I

L

M

L

V

I

T

T

H

H

D

E

A

M

R

D

L

V

L

V

P

K

-

R

Y

I

C

C

D

D

K

C

I

V

L

A

S

V

I

I

E

S

N

N

R

G

K

E

V

L

V

I

T

E

K

Q

T

D

Q

T

V

V

A

S

Q

E

E

V

F

F

binding adenosine-5'-diphosphate: F12 (= F15), Q14 (≠ K17), G42 (= G45), G44 (= G47), K45 (= K48), S46 (≠ T49), T47 (= T50)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
33% identity, 97% coverage: 6:236/237 of query aligns to 3:230/344 of 3tuiC

query
sites
3tuiC

I

I

R

K

V

L

E

S

N

N

L

I

T

T

K

K

I

V

F
|
F

G

H

K

Q

G

G

D

T

A

R

Q

T

V

I

R

Q

V

A

L

L

E

N

N

N

A

V

S

S

L

L

E

H

V

V

K

P

K

A

G

G

E

Q

F

I

A

Y

A

G

L

V

V

I

A

G

P

A

S

S

G
|
G

A
|
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

M

R

M

C

I

V

G

N

C

L

V

L

E

E

K

R

P

P

T

T

S

E

G

G

K

S

I

V

W

L

L

V

-

D

G

G

D

Q

E

E

L

L

V

T

W

T

D

-

E

-

D

-

H

-

W

L

S

S

I

E

R

S

D

E

P

L

R

T

K

K

I

A

R

R

R

R

E

Q

K

-

L

I

G

G

F

M

I

I

F

F

Q

Q

A

H

H

F

Y

N

L

L

I

L

P

S

F

S

L

R

N

T

I

V

L

F

E

G

N

N

V

V

I

A

L

L

I

P

P

L

T

E

T

L

N

D

G

N

I

T

P

P

R

K

K

D

A

E

A

V

E

K

K

R

K

R

A

V

M

T

E

E

L

L

L

L

D

S

Y

L

F

V

D

G

I

L

G

G

D

D

K

K

A

H

H

D

A

S

M

Y

P

P

S

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

N

K

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

A

N

S

D

N

P

P

H

K

I

V

V

L

L

L

A

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

D

D

M

P

S

A

R

T

A

T

V

R

N

S

V

I

V

L

K

E

M

L

L

L

R

K

K

D

I

I

A

N

K

R

E

R

R

L

Q

G

V

L

A

T

I

I

I

L

M

L

V

I

T

T

H

H

D

E

A

M

R

D

L

V

L

V

P

K

-

R

Y

I

C

C

D

D

K

C

I

V

L

A

S

V

I

I

E

S

N

N

R

G

K

E

V

L

V

I

T

E

K

Q

T

D

Q

T

V

V

A

S

Q

E

E

V

F

F

binding adenosine-5'-diphosphate: F12 (= F15), G42 (= G45), A43 (= A46), G44 (= G47), K45 (= K48), S46 (≠ T49), T47 (= T50)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
33% identity, 97% coverage: 6:236/237 of query aligns to 3:230/344 of 6cvlD

query
sites
6cvlD

I

I

R

K

V

L

E

S

N

N

L

I

T

T

K

K

I

V

F
|
F

G

H

K
x
Q

G

G

D

T

A

R

Q

T

V
x
I

R

Q

V

A

L

L

E

N

N

N

A

V

S

S

L

L

E

H

V

V

K

P

K

A

G

G

E

Q

F

I

A

Y

A

G

L

V

V

I

A

G

P

A

S
|
S

G
|
G

A
|
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

M

R

M

C

I

V

G

N

C

L

V

L

E

E

K

R

P

P

T

T

S

E

G

G

K

S

I

V

W

L

L

V

-

D

G

G

D

Q

E

E

L

L

V

T

W

T

D

-

E

-

D

-

H

-

W

L

S

S

I

E

R

S

D

E

P

L

R

T

K

K

I

A

R

R

R

R

E

Q

K

-

L

I

G

G

F

M

I

I

F

F

Q

Q

A

H

H

F

Y

N

L

L

I

L

P

S

F

S

L

R

N

T

I

V

L

F

E

G

N

N

V

V

I

A

L

L

I

P

P

L

T

E

T

L

N

D

G

N

I

T

P

P

R

K

K

D

A

E

A

V

E

K

K

R

K

R

A

V

M

T

E

E

L

L

L

L

D

S

Y

L

F

V

D

G

I

L

G

G

D

D

K

K

A

H

H

D

A

S

M

Y

P

P

S

S

Q
x
N

L

L

S
|
S

G

G

G

G

Q
|
Q

N

K

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

A

N

S

D

N

P

P

H

K

I

V

V

L

L

L

A

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

D

D

M

P

S

A

R

T

A

T

V

R

N

S

V

I

V

L

K

E

M

L

L

L

R

K

K

D

I

I

A

N

K

R

E

R

R

L

Q

G

V

L

A

T

I

I

I

L

M

L

V

I

T

T

H
|
H

D

E

A

M

R

D

L

V

L

V

P

K

-

R

Y

I

C

C

D

D

K

C

I

V

L

A

S

V

I

I

E

S

N

N

R

G

K

E

V

L

V

I

T

E

K

Q

T

D

Q

T

V

V

A

S

Q

E

E

V

F

F

binding phosphothiophosphoric acid-adenylate ester: F12 (= F15), Q14 (≠ K17), I19 (≠ V22), S41 (= S44), G42 (= G45), A43 (= A46), G44 (= G47), K45 (= K48), S46 (≠ T49), T47 (= T50), N141 (≠ Q148), S143 (= S150), Q146 (= Q153), H200 (= H207)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
37% identity, 97% coverage: 6:236/237 of query aligns to 3:224/241 of 4u00A

query
sites
4u00A

I

I

R

R

V

I

E

R

N

N

L

L

T

H

K

K

I

W

F
|
F

G

G

K

P

G

-

D

-

A

-

Q

-

V

L

R

H

V
|
V

L

L

E

K

N

G

A

I

S

H

L

L

E

E

V

V

K

A

K

P

G

G

E

E

F

K

A

L

A

V

L

I

V

I

A

G

P

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

M

R

M

T

I

I

G

N

C

R

V

L

E

E

K

D

P

F

T

Q

S

E

G

G

K

E

I

V

W

V

L

V

G

-

D

-

E

-

L

-

V

-

W

-

D

-

E

-

D

D

H

G

W

L

S

S

I

V

R

K

D

D

P

D

R

R

K

A

I

L

R

R

-

E

-

I

R

R

E

R

K

E

L

V

G

G

F

M

I

V

F

F

Q

Q

A

Q

H

F

Y

N

L

L

I

F

P

P

F

H

L

M

N

T

I

V

L

L

E

E

N

N

V

V

I

T

L

L

I

A

P

P

T

M

-

R

T

V

N

R

G

R

I

W

P

P

R

R

K

E

A

K

A

A

E

E

K

K

K

K

A

A

M

L

E

E

L

L

L

L

D

E

Y

R

F

V

D

G

I

I

G

L

D

D

K

Q

A

A

H

R

A

K

M

Y

P

P

S

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

N

Q

Q

Q

R

R

A

V

A

A

I

I

A

A

R

R

A

A

L

L

S

A

N

M

D

E

P

P

H

K

I

I

V

M

L

L

A

F

D

D

E

E

P

P

T

T

A

S

A

A

L

L

D

D

M

P

S

E

R

M

A

V

V

G

N

E

V

V

V

L

K

D

M

V

L

M

R

R

K

D

I

L

A

A

K

Q

E

G

R

-

Q

G

V

M

A

T

I

M

I

V

M

V

V

V

T

T

H

H

D

E

A

M

R

G

L

F

L

A

-

R

P

E

Y

V

C

A

D

D

K

R

I

V

L

V

S

F

I

M

E

D

N

G

R

G

K

Q

V

I

V

V

T

E

K

E

T

G

Q

R

V

P

A

E

Q

E

E

I

F

F

binding adenosine-5'-diphosphate: F12 (= F15), V17 (= V24), S37 (= S44), G38 (= G45), S39 (≠ A46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50)

7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
37% identity, 94% coverage: 6:227/237 of query aligns to 5:216/218 of 7w78A

query
sites
7w78A

I

L

R

S

V

I

E

T

N

N

L

A

T

S

K

V

I

V

F
x
Y

G

P

K

D

G

G

D

I

A

S

Q

T

V

V

R

T

V

A

L

L

E

D

N

S

A

A

S

N

L

V

E

E

V

I

K

F

K

P

G

G

E

E

F

L

A

V

A

A

L

I

V

V

A

G

P

E

S
|
S

G
|
G

A

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

M

S

M

I

I

A

G

G

C

F

V

L

E

Q

K

E

P

P

T

T

S

S

G

G

K

T

I

V

W

T

L

L

-

H

G

G

D

A

E

E

L

G

V

L

W

-

D

-

E

-

D

-

H

-

W

-

S

-

I

-

R

-

D

D

P

A

R

T

K

S

I

T

R

R

R

R

E

E

K

H

L

I

G

G

F

F

I

V

F

F

Q
|
Q

A

Q

H

P

Y

N

L

L

I

L

P

G

F

S

L

L

N

T

I

A

L

R

E

E

N

Q

V

L

I

L

L

I

I

T

P

D

T

H

T

L

N

R

G

G

I

I

-

K

P

P

R

R

K

K

A

-

A

-

E

-

K

D

K

R

A

A

M

D

E

E

L

L

L

L

D

A

Y

R

F

V

D

G

I

L

G

K

D

G

K

L

A

G

H

G

A

R

M

R

P

V

S

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

N

R

Q

Q

R

R

A

V

A

N

I

I

A

A

R

R

A

A

L

L

S

M

N

G

D

N

P

P

H

Q

I

L

V

L

L

L

A

A

D

D

E
|
E

P

P

T

T

A

S

A

A

L

L

D

D

M

A

S

R

R

L

A

S

V

K

N

E

V

I

V

V

K

E

M

L

L

L

R

R

K

D

I

V

A

T

K

K

E

E

R

F

Q

A

V

L

A

A

I

T

I

L

M

M

V

V

T

T

H

H

D

D

A

R

R

S

L

Q

L

L

P

A

Y

Y

C

A

D

D

K

R

I

F

L

V

S

E

I

M

E

A

N

D

R

G

K

K

V

A

V

L

binding phosphoaminophosphonic acid-adenylate ester: Y14 (≠ F15), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (≠ T49), T49 (= T50), Q88 (= Q97), E163 (= E174)
binding magnesium ion: S48 (≠ T49), Q88 (= Q97)

7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
37% identity, 94% coverage: 6:227/237 of query aligns to 5:216/216 of 7w79A

query
sites
7w79A

I

L

R
x
S

V

I

E

T

N

N

L

A

T

S

K

V

I

V

F
x
Y

G

P

K

D

G

G

D

I

A

S

Q

T

V

V

R

T

V

A

L

L

E

D

N

S

A

A

S

N

L

V

E
|
E

V

I

K

F

K

P

G

G

E

E

F

L

A

V

A

A

L

I

V

V

A

G

P

E

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

M

S

M

I

I

A

G

G

C

F

V

L

E

Q

K

E

P

P

T

T

S

S

G

G

K

T

I

V

W

T

L

L

-

H

G

G

D

A

E

E

L

G

V

L

W

-

D

-

E

-

D

-

H

-

W

-

S

-

I

-

R

-

D

D

P

A

R

T

K

S

I

T

R

R

R

R

E

E

K

H

L

I

G

G

F

F

I

V

F

F

Q
|
Q

A

Q

H

P

Y

N

L

L

I

L

P

G

F

S

L

L

N

T

I

A

L

R

E

E

N

Q

V

L

I

L

L

I

I

T

P

D

T

H

T

L

N

R

G

G

I

I

-

K

P

P

R

R

K

K

A

-

A

-

E

-

K

D

K

R

A

A

M

D

E

E

L

L

L

L

D

A

Y

R

F

V

D

G

I

L

G

K

D

G

K

L

A

G

H

G

A

R

M

R

P

V

S

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

N

R

Q

Q

R

R

A

V

A

N

I

I

A

A

R

R

A

A

L

L

S

M

N

G

D

N

P

P

H

Q

I

L

V

L

L

L

A

A

D

D

E
|
E

P

P

T

T

A

S

A

A

L

L

D

D

M

A

S

R

R

L

A

S

V

K

N

E

V

I

V

V

K

E

M

L

L

L

R

R

K

D

I

V

A

T

K

K

E

E

R

F

Q

A

V

L

A

A

I

T

I

L

M

M

V

V

T

T

H
|
H

D

D

A

R

R

S

L

Q

L

L

P

A

Y

Y

C

A

D

D

K

R

I

F

L

V

S

E

I

M

E

A

N

D

R

G

K

K

V

A

V

L

binding adenosine-5'-triphosphate: Y14 (≠ F15), S43 (= S44), G44 (= G45), S45 (≠ A46), G46 (= G47), K47 (= K48), S48 (≠ T49), T49 (= T50)
binding manganese (ii) ion: S6 (≠ R7), E30 (= E31), S48 (≠ T49), Q88 (= Q97), E163 (= E174), H196 (= H207)

Query Sequence

>WP_013553508.1 NCBI__GCF_000186245.1:WP_013553508.1
MNDTGIRVENLTKIFGKGDAQVRVLENASLEVKKGEFAALVAPSGAGKTTLLMMIGCVEK
PTSGKIWLGDELVWDEDHWSIRDPRKIRREKLGFIFQAHYLIPFLNILENVILIPTTNGI
PRKAAEKKAMELLDYFDIGDKAHAMPSQLSGGQNQRAAIARALSNDPHIVLADEPTAALD
MSRAVNVVKMLRKIAKERQVAIIMVTHDARLLPYCDKILSIENRKVVTKTQVAQEFI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory