SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013554179.1 NCBI__GCF_000186245.1:WP_013554179.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
59% identity, 99% coverage: 5:311/311 of query aligns to 8:318/318 of Q63XL8

query
sites
Q63XL8

M

M

L

V

F

F

S

T

G

G

T

N

A

A

N

N

P

P

P

A

L

L

S

A

Q

Q

A

E

I

V

A

V

D

K

Y

I

L

L

E

G

M

I

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P

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L

S

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G

K

A

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D

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G

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E

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T

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P

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G

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Y

A

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R

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K

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R

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D

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S

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D

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K

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P

K

K

A

A

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N

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A

A

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E

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M

M

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N

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I

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G

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C

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K

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E

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S

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M

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A

E

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S

RQ 99:100 (= RQ 96:97) binding ATP

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
56% identity, 99% coverage: 1:308/311 of query aligns to 1:313/315 of P0A717

query
sites
P0A717

M
|
M

S

P

N

D

Y

M

M

K

L

L

F

F

S

A

G

G

T

N

A

A

N

T

P

P

P

E

L

L

S

A

Q

Q

A

R

I

I

A

A

D

N

Y

R

L

L

E

Y

M

T

P

S

L

L

S

G

G

D

A

A

T

A

I

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N

G

R

R

F

F

S

S

D

D

G

G

E

E

I

V

N

S

V

V

Q

Q

I

I

A

N

E

E

S

N

V

V

R

R

G

G

K

G

D

D

V

I

F

F

I

I

Q

Q

P

S

T

T

C

C

A

A

P

P

A

T

N

N

A

D

N

N

L

L

M

M

E

E

L

L

L

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I

V

M

M

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D

A

A

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D

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G

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I

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V

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D

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Y

F

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G

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Y

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D

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D

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R

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D

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D

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D

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A

G

G

G

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T

M

L

V

C

K

K

G

A

A

A

A

E

A

A

L

L

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K

E

E

A

R

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G

A

A

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A

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C

Y

C

A

T

T

H

H

P

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L

F

S

S

G

G

P

N

A

A

I

A

D

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R

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D

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C

D

D

T

T

I

I

P

P

L

L

K

S

A

D

E

E

C

I

K

K

-

S

-

L

-

P

K

N

I

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K

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L

L

S

T

T

L

A

S

Q

G

M

M

L

L

G

A

E

E

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A

I

I

R

R

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S

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N

N

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A

L

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F

F

M1 (= M1) modified: Initiator methionine, Removed
D129 (= D128) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D218) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D219) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D222) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

6asvC E. Coli prpp synthetase (see paper)
57% identity, 97% coverage: 6:308/311 of query aligns to 4:311/311 of 6asvC

query
sites
6asvC

L

L

F

F

S

A

G

G

T

N

A

A

N

T

P

P

P

E

L

L

S

A

Q

Q

A

R

I

I

A

A

D

N

Y

R

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L

E

Y

M

T

P

S

L

L

S

G

G

D

A

A

T

A

I

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N

G

R

R

F

F

S

S

D

D

G

G

E

E

I

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V

Q

Q

I

I

A

N

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E

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N

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V

R

R

G

G

K

G

D

D

V

I

F

F

I

I

Q

Q

P

S

T

T

C

C

A

A

P

P

A

T

N

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A

D

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L

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M

E

E

L

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D

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F

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G

F

F

D

D

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D

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A

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A

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I

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D

D

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D

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A

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I

I

G

G

D

D

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A

G

G

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D

D

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D

M

M

V

I

D
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D

T
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T

A
x
G

G

G

T
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T

M
x
L

V

C

K

K

G

A

A

A

A

E

A

A

L

L

K

K

E

E

A

R

G

G

A

A

T

K

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M

F

A

A

C

Y

C

A

T

T

H

H

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G

P

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A

A

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A

D

N

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S

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D

D

E

E

L

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C

D

D

T

T

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A

D

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E

C

I

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K

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-

L

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L

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T

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S

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G

M

M

L

L

G

A

E

E

V

A

I

I

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Q

S

N

N

N

E

E

E

S

S

V

I

N

S

S

A

L

M

F

F

binding phosphate ion: D168 (= D169), D218 (= D218), D219 (= D219), D222 (= D222), T223 (= T223), G224 (≠ A224), T226 (= T226), L227 (≠ M227)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
57% identity, 96% coverage: 6:304/311 of query aligns to 5:308/308 of 4s2uA

query
sites
4s2uA

L

L

F

F

S

A

G

G

T

N

A

A

N

T

P

P

P

E

L

L

S

A

Q

Q

A

R

I

I

A

A

D

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Y

R

L

L

E

Y

M

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S

L

L

S

G

G

D

A

A

T

A

I

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G

R

R

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F

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S

D

D

G

G

E

E

I

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S

V

V

Q

Q

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A

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V

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R

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G

K

G

D

D

V

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F

F

I

I

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Q

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T

C

C

A

A

P

P

A

T

N

N

A

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

T

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D

D

A

A

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L

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A

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S

A

A

K

G

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A

A

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V

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P

Y

Y

Y

F

G

G

Y

Y

A

A

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Q

D

D

R

R

K

R

A

V

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R

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A

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P

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A

A

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A

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D

D

L

L

H

H

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E

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Q

G

G

F

F

D

D

I

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P

P

V

V

D

D

N

N

L

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Y

F

G

G

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S

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P

L

I

F

L

I

L

D

E

Y

D

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K

L

N

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F

L

A

D

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P

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A

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S

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P

D

D

I

I

G

G

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A

V

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R

A

A

R

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Y

A

F

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A

A

S

K

R

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L

L

G

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-

D

M

T

D

D

M

M

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A

I

I

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I

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D

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K

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R

A

A

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V

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M

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H

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G

G

D

D

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A

G

G

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D

D

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D

M

M

V

I

D
|
D

T

T

A

G

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T

M

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C

K

K

G

A

A

A

A

E

A

A

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K

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E

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G

A

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K

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A

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Y

C

A

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H

H

P

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S

G

G

P

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A

A

I

A

D

N

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R

D

N

G

S

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D

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E

L

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C

D

D

T

T

I

I

P

P

L

L

K

S

A

D

E

E

C

I

K

K

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S

-

L

-

P

K

N

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V

K

R

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L

L

S

T

T

L

A

S

Q

G

M

M

L

L

G

A

E

E

V

A

I

I

R

R

V

I

Q

S

N

N

N

E

E

E

S

S

V

I

binding magnesium ion: D223 (= D222), T227 (= T226)

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
57% identity, 96% coverage: 5:304/311 of query aligns to 3:299/300 of 3dahC

query
sites
3dahC

M

M

L

V

F

F

S

T

G

G

T

N

A

A

N

N

P

P

P

A

L

L

S

A

Q

Q

A

E

I

V

A

V

D

K

Y

I

L

L

E

G

M

I

P

P

L

L

S

G

G

K

A

A

T

M

I

V

N

S

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

I

N

Q

V

V

Q

E

I

I

A

Q

E

E

S

N

V

V

R

R

G

G

K

K

D

D

V

V

F

F

I

V

I

L

Q

Q

P

S

T

T

C

C

A

A

P

P

A

T

N

N

A

D

N

N

L

L

M

M

E

E

L

L

L

M

I

I

M

M

T

V

D

D

A

A

L

L

K

K

R

R

S

A

S

S

A

A

K

G

S

R

I

I

T

T

A

A

V

A

V

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

A

P

-

R

A

S

P

A

R

R

V

V

P

A

I

I

T

S

A

A

K

K

L

V

V

V

A

A

D

N

M

M

M

L

E

E

K

I

A

A

G

G

I

V

T

E

R

R

V

I

T

T

V

M

D

D

L

L

H

H

A

A

S

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

I

Y

Y

G

A

S

T

I

P

L

I

F

L

I

L

D

G

Y

D

I

L

R

R

S

K

K

Q

N

N

F

Y

A

P

N

D

P

L

I

L

I

V

A

V

S

S

P

P

D

D

I

V

G

G

V

V

A

V

R

R

A

A

R

R

Y

A

F

L

A

A

S

K

R

Q

L

L

G

N

M

C

D

D

M

L

V

A

I

I

V

I

D

D

K

K

R

R

E

-

K

-

A

-

N

-

V

-

A

-

E

-

V

V

M

M

N

N

I

I

G

G

D

E

V

V

R

E

G

G

R

R

D

T

V

C

I

V

L

I

I

M

D

D

M

M

V

V

D

D

T

T

A

A

G

G

T

T

M

L

V

C

K

K

G

A

A

A

A

Q

A

V

L

L

K

K

E

E

A

R

G

G

A

A

T

K

S

Q

V

V

M

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

G

A

A

I

A

D

D

R

R

I

I

R

A

D

A

G

S

A

A

L

L

D

D

E

E

L

L

V

V

S

T

D

D

T

T

I

I

P

P

L

L

K

S

A

A

E

E

-

S

-

L

-

A

C

C

K

P

K

K

I

I

K

R

V

A

L

L

S

S

T

S

A

A

Q

G

M

L

L

L

G

A

E

E

V

T

I

F

R

S

R

R

V

I

Q

R

N

R

N

G

E

D

S

S

V

V

binding adenosine monophosphate: F33 (= F35), D35 (= D37), E37 (= E39), R94 (= R96), Q95 (= Q97)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
56% identity, 97% coverage: 6:308/311 of query aligns to 4:305/307 of 7xmvA

query
sites
7xmvA

L

L

F

F

S

A

G

G

T

N

A

A

N

T

P

P

P

E

L

L

S

A

Q

Q

A

R

I

I

A

A

D

N

Y

R

L

L

E

Y

M

T

P

S

L

L

S

G

G

D

A

A

T

A

I

V

N

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

N

S

V

V

Q

Q

I

I

A

N

E

E

S

N

V

V

R

R

G

G

K

G

D

D

V

I

F

F

I

I

Q

Q

P

S

T

T

C

C

A

A

P

P

A

T

N

N

A

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

T

V

D

D

A

A

L

L

K

R

R

R

S

A

S

S

A

A

K

G

S

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

K

R

A
x
V

-
x
R

A

S

P

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

D

D

M

F

M

L

E

S

K

S

A

V

G

G

I

V

T

D

R

R

V

V

V

L

T

T

V

V

D

D

L

L

H
|
H

A

A

S
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

L

V

Y
x
F

G

G

S
|
S

I

P

L

I

F

L

I

L

D

E

Y

D

I

M

R

L

S

Q

K

L

N

N

F

L

A

D

N

N

P

P

I

I

I

V

A

V

S

S

P

P

D

D

I

I

G

G

V

V

A
x
V

R

R

A

A

R

R

Y
x
A

F

I

A

A

S

K

R

L

L

L

G

N

-

D

M

T

D

D

M

M

V

A

I

I

V

I

D

D

K

K

R

R

E

-

K

-

A

-

N

-

V

-

A

-

E

Q

V

V

M

M

N

H

I

I

G

G

D

D

V

V

R

A

G

G

R

R

D

D

V

C

I

V

L

L

I

V

D
|
D

M
|
M

V

I

D
|
D

T
|
T

A

G

G
|
G

T
|
T

M

L

V

C

K

K

G

A

A

A

A

E

A

A

L

L

K

K

E

E

A

R

G

G

A

A

T

K

S

R

V

V

M

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

D

N

R

N

I

L

R

R

D

N

G

S

A

V

L

I

D

D

E

E

L

V

V

V

S

C

D

D

T

T

I

I

P

P

L

L

K

S

A

D

E

E

C

I

K

K

-

S

-

L

-

P

K

N

I

V

K

R

V

T

L

L

S

T

T

L

A

S

Q

G

M

M

L

L

G

A

E

E

V

A

I

I

R

R

V

I

Q

S

N

N

N

E

E

E

S

S

V

I

N

S

S

A

L

M

F

F

binding adenosine-5'-diphosphate: F33 (= F35), D35 (= D37), E37 (= E39), R94 (= R96), R97 (= R99), H129 (= H130)
binding adenosine monophosphate: R97 (= R99), V99 (≠ A101), R100 (vs. gap), E131 (≠ S132), F145 (≠ Y146), S147 (= S148), V173 (≠ A174), A177 (≠ Y178)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D218), D213 (= D219), M214 (= M220), D216 (= D222), T217 (= T223), G219 (= G225), T220 (= T226)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
56% identity, 97% coverage: 6:308/311 of query aligns to 4:305/307 of 7xmuA

query
sites
7xmuA

L

L

F

F

S

A

G

G

T

N

A

A

N

T

P

P

P

E

L

L

S

A

Q

Q

A

R

I

I

A

A

D

N

Y

R

L

L

E

Y

M

T

P

S

L

L

S

G

G

D

A

A

T

A

I

V

N

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

N

S

V

V

Q

Q

I

I

A

N

E

E

S

N

V

V

R

R

G

G

K

G

D

D

V

I

F

F

I

I

Q

Q

P

S

T

T

C

C

A

A

P

P

A

T

N

N

A

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

T

V

D

D

A

A

L

L

K

R

R

R

S

A

S

S

A

A

K

G

S

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

R

A

V

-
x
R

A

S

P

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

D

D

M

F

M

L

E

S

K

S

A

V

G

G

I

V

T

D

R

R

V

V

V

L

T

T

V

V

D

D

L

L

H
|
H

A

A

S
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

L

V

Y
x
F

G

G

S
|
S

I

P

L

I

F

L

I

L

D

E

Y

D

I

M

R

L

S

Q

K

L

N

N

F

L

A

D

N

N

P

P

I

I

I

V

A

V

S

S

P

P

D
|
D

I

I

G

G

V

V

A
x
V

R

R

A

A

R

R

Y

A

F

I

A

A

S

K

R

L

L

L

G

N

-

D

M

T

D

D

M

M

V

A

I

I

V

I

D

D

K

K

R

R

E

-

K

-

A

-

N

-

V

-

A

-

E

Q

V

V

M

M

N

H

I

I

G

G

D

D

V

V

R

A

G

G

R

R

D

D

V

C

I

V

L

L

I

V

D
|
D

D

D

M
|
M

V

I

D
|
D

T
|
T

A

G

G

G

T

T

M

L

V

C

K

K

G

A

A

A

A

E

A

A

L

L

K

K

E

E

A

R

G

G

A

A

T

K

S

R

V

V

M

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

D

N

R

N

I

L

R

R

D

N

G

S

A

V

L

I

D

D

E

E

L

V

V

V

S

C

D

D

T

T

I

I

P

P

L

L

K

S

A

D

E

E

C

I

K

K

-

S

-

L

-

P

K

N

I

V

K

R

V

T

L

L

S

T

T

L

A

S

Q

G

M

M

L

L

G

A

E

E

V

A

I

I

R

R

V

I

Q

S

N

N

N

E

E

E

S

S

V

I

N

S

S

A

L

M

F

F

binding adenosine-5'-diphosphate: F33 (= F35), D35 (= D37), E37 (= E39), R94 (= R96), Q95 (= Q97), R97 (= R99), R97 (= R99), R100 (vs. gap), H129 (= H130), E131 (≠ S132), F145 (≠ Y146), S147 (= S148), V173 (≠ A174)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D169), D212 (= D218), M214 (= M220), D216 (= D222), T217 (= T223)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
57% identity, 96% coverage: 2:299/311 of query aligns to 1:298/299 of 6nfeB

query
sites
6nfeB

S

S

N

T

Y

M

M

M

L

I

F

F

S

T

G

G

T

N

A

A

N

N

P

P

P

E

L

L

S

A

Q

L

A

K

I

I

A

S

D

S

Y

H

L

L

E

Q

M

I

P

P

L

I

S

G

G

K

A

A

T

T

I

V

N

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

N

M

V

V

Q

E

I

I

A

L

E

E

S

N

V

V

R

R

G

G

K

K

D

D

V

V

F

F

I

V

I

L

Q

Q

P

S

T

T

C

C

A

A

P

P

A

A

N

N

A

N

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

A

D

D

A

A

L

L

K

R

R

R

S

S

A

A

K

G

S

R

I

I

T

T

A

A

V

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

A

V

-

R

A

S

P

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

D

D

M

M

E

A

K

S

A

V

G

G

I

I

T

C

R

R

V

V

V

L

T

T

V

V

D

D

L

L

H
|
H

A

A

S

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

L

V

Y

Y

G

S

S

T

I

P

L

V

F

L

I

L

D

E

Y

D

I

I

R

T

S

K

K

Q

N

K

F

L

A

N

N

N

P

I

I

M

I

I

A

V

S

S

P

P

D

D

I

V

G

G

V

V

A

V

R

R

A

A

R

R

Y

A

F

V

A

A

S

K

R

R

L

L

G

N

-

D

M

A

D

E

M

L

V

S

I

I

V

I

D

D

K

K

R

R

E

-

K

-

A

-

N

-

V

-

A

S

E

E

V

V

M

M

N

H

I

I

G

G

D

E

V

P

R

A

G

N

R

K

D

N

V

C

I

I

L

I

I

V

D
|
D

M
x
I

V

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

M

L

V

C

K

T

G

A

A

A

A

H

A

E

L

L

K

K

E

K

A

N

G

G

A

A

T

K

S

S

V

V

M

R

A

A

C

Y

C

I

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

D

N

R

N

I

I

R

K

D

H

G

S

A

G

L

L

D

D

E

E

L

V

V

V

S

T

D

D

T

T

I

I

P

P

L

L

K

S

A

A

E

E

-

A

-

Q

-

N

C

C

K

E

K

K

I

I

K

R

V

V

L

V

S

S

T

L

A

A

Q

D

M

M

L

L

G

A

E

Q

V

A

I

I

R

K

R

R

V

V

Q

N

binding adenosine-5'-diphosphate: F34 (= F35), D36 (= D37), E38 (= E39), R95 (= R96), Q96 (= Q97), H130 (= H130)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H130), D214 (= D218), D215 (= D219), I216 (≠ M220), D218 (= D222), T219 (= T223), A220 (= A224), T222 (= T226)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
54% identity, 99% coverage: 3:309/311 of query aligns to 9:317/317 of P14193

query
sites
P14193

N

N

Y

L

M

K

L

I

F

F

S

S

G

L

T

N

A

S

N

N

P

P

P

E

L

L

S

A

Q

K

A

E

I

I

A

A

D

D

Y

I

L

V

E

G

M

V

P

Q

L

L

S

G

G

K

A

C

T

S

I

V

N

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

N

Q

V

I

Q

N

I

I

A

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

F

Y

I

I

Q

Q

P

S

T

T

C

S

A

D

P

P

A

V

N

N

A

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

T

V

D

D

A

A

L

L

K

K

R

R

S

A

S

S

A

A

K

K

S

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

K

A

A

A

R

P

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

D

N

M

L

E

E

K

T

A

A

G

G

I

A

T

T

R

R

V

V

V

I

T

A

V

L

D

D

L

L

H

H

A

A

S

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

L

L

Y

M

G

G

S

V

I

P

L

I

F

L

I

G

D

E

Y

Y

I

F

R

E

S

G

K

K

N

N

F

L

A

E

N

D

P

I

I

V

I

I

A

V

S

S

P

P

D

D

I

H

G

G

V

V

A

T

R

R

A

A

R

R

Y

K

F

L

A

A

S

D

R

R

L

L

G

K

M

A

D

P

M

I

V

A

I

I

V

I

D

D

K
|
K

R

R

R
|
R

E

P

K
x
R

A

P

N
|
N

V

V

A

A

E
|
E

V

V

M

M

N

N

I

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

V

A

I

I

L

L

I

I

D

D

M

I

V

I

D
|
D

T
|
T

A
|
A

G
|
G

T
|
T

M

I

V

T

K

L

G

A

A

A

A

N

A

A

L

L

K

V

E

E

A

N

G

G

A

A

T

K

S

E

V

V

M

Y

A

A

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

D

E

R

R

I

I

R

N

D

N

G

S

A

T

L

I

D

K

E

E

L

L

V

V

S

T

D

N

T

S

I

I

P

K

L

L

K

P

A

E

E

E

C

K

K

K

-

I

-

E

K

R

I

F

K

K

V

Q

L

L

S

S

T

V

A

G

Q

P

M

L

L

L

G

A

E

E

V

A

I

I

R

I

R

R

V

V

Q

H

N

E

N

Q

E

Q

S

S

V

V

N

S

S

Y

L

L

F

F

A

S

RQ 102:103 (= RQ 96:97) binding ATP
K198 (= K192) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R194) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ K196) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N198) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E201) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (= DTAGT 222:226) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
57% identity, 96% coverage: 2:299/311 of query aligns to 1:297/298 of 6nfeA

query
sites
6nfeA

S

S

N

T

Y

M

M

M

L

I

F

F

S

T

G

G

T

N

A

A

N

N

P

P

P

E

L

L

S

A

Q

L

A

K

I

I

A

S

D

S

Y

H

L

L

E

Q

M

I

P

P

L

I

S

G

G

K

A

A

T

T

I

V

N

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

N

M

V

V

Q

E

I

I

A

L

E

E

S

N

V

V

R

R

G

G

K

K

D

D

V

V

F

F

I

V

I

L

Q

Q

P

S

T

T

C

C

A

A

P

P

A

A

N

N

A

N

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

A

D

D

A

A

L

L

K

R

R

R

S

S

A

A

K

G

S

R

I

I

T

T

A

A

V

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

R

A
x
V

-

R

A

S

P

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

D

D

M

M

E

A

K

S

A

V

G

G

I

I

T

C

R

R

V

V

V

L

T

T

V

V

D

D

L

L

H
|
H

A

A

S

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

L

V

Y
|
Y

G

S

S

T

I

P

L

V

F

L

I

L

D

E

Y

D

I

I

R

T

S

K

K

Q

N

K

F

L

A

N

N

N

P

I

I

M

I

I

A

V

S

S

P

P

D

D

I

V

G

G

V

V

A

V

R
|
R

A

A

R

R

Y
x
A

F

V

A

A

S
x
K

R

R

L

L

G

N

-

D

M

A

D

E

M

L

V

S

I

I

V

I

D

D

K

K

R

R

E

-

K

-

A

-

N

-

V

-

A

-

E

E

V

V

M

M

N

H

I

I

G

G

D

E

V

P

R

A

G

N

R

K

D

N

V

C

I

I

L

I

I

V

D
|
D

M
x
I

V

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

M

L

V

C

K

T

G

A

A

A

A

H

A

E

L

L

K

K

E

K

A

N

G

G

A

A

T

K

S

S

V

V

M

R

A

A

C

Y

C

I

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

D

N

R

N

I

I

R

K

D

H

G

S

A

G

L

L

D

D

E

E

L

V

V

V

S

T

D

D

T

T

I

I

P

P

L

L

K

S

A

A

E

E

-

A

-

Q

-

N

C

C

K

E

K

K

I

I

K

R

V

V

L

V

S

S

T

L

A

A

Q

D

M

M

L

L

G

A

E

Q

V

A

I

I

R

K

R

R

V

V

Q

N

binding adenosine-5'-diphosphate: F34 (= F35), D36 (= D37), E38 (= E39), R95 (= R96), Q96 (= Q97), H130 (= H130)
binding adenosine monophosphate: R98 (= R99), V100 (≠ A101), Y146 (= Y146), R175 (= R175), A178 (≠ Y178), K181 (≠ S181)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H130), D213 (= D218), D214 (= D219), I215 (≠ M220), D217 (= D222), T218 (= T223), A219 (= A224), T221 (= T226)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
53% identity, 98% coverage: 3:308/311 of query aligns to 1:295/295 of 1dkuA

query
sites
1dkuA

N

N

Y

L

M

K

L

I

F

F

S

S

G

L

T

N

A

S

N

N

P

P

P

E

L

L

S

A

Q

K

A

E

I

I

A

A

D

D

Y

I

L

V

E

G

M

V

P

Q

L

L

S

G

G

K

A

C

T

S

I

V

N

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

N

Q

V

I

Q

N

I

I

A

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

F

Y

I

I

Q

Q

P

S

T

T

C

S

A

D

P

P

A

V

N

N

A

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

T

V

D

D

A

A

L

L

K

K

R

R

S

A

S

S

A

A

K

K

S

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R

R

K
|
K

A

A

A

R

P
x
S

R
|
R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

D

N

M

L

E

E

K

T

A

A

G

G

I

A

T

T

R

R

V

V

V

I

T

A

V

L

D

D

L

L

H
|
H

A

A

S

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D
|
D

N

H

L

L

Y

M

G

G

S

V

I

P

L

I

F

L

I

G

D

E

Y

Y

I

F

R

E

S

G

K

K

N

N

F

L

A

E

N

D

P

I

I

V

I

I

A

V

S

S

P

P

D

D

I

H

G

G

V

V

A

T

R

R

A

A

R

R

Y

K

F

L

A

A

S

D

R

R

L

L

G

K

M

A

D

P

M

I

V

A

I

I

V

I

D

D

K

K

R

R

R

-

E

-

K

-

A

-

N

-

V

-

A

-

E

-

V

-

M

M

N

N

I

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

V

A

I

I

L

L

I

I

D

D

M

I

V

I

D

D

T

T

A

A

G

G

T

T

M

I

V

T

K

L

G

A

A

A

A

N

A

A

L

L

K

V

E

E

A

N

G

G

A

A

T

K

S

E

V

V

M

Y

A

A

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

D

E

R

R

I

I

R

N

D

N

G

S

A

T

L

I

D

K

E

E

L

L

V

V

S

T

D

N

T

S

I

I

P

K

L

L

K

K

A

I

E

E

C

-

K

-

K

R

I

F

K

K

V

Q

L

L

S

S

T

V

A

G

Q

P

M

L

L

L

G

A

E

E

V

A

I

I

R

I

R

R

V

V

Q

H

N

E

N

Q

E

Q

S
|
S

V
|
V

N
x
S

S

Y

L

L

F
|
F

binding methyl phosphonic acid adenosine ester: R94 (= R96), H128 (= H130)
binding phosphomethylphosphonic acid adenosyl ester: K98 (= K100), S101 (≠ P103), R102 (= R104), D141 (= D143), S290 (= S303), V291 (= V304), S292 (≠ N305), F295 (= F308)

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
52% identity, 98% coverage: 3:308/311 of query aligns to 3:299/299 of 1ibsB

query
sites
1ibsB

N

N

Y

L

M

K

L

I

F

F

S

S

G

L

T

N

A

S

N

N

P

P

P

E

L

L

S

A

Q

K

A

E

I

I

A

A

D

D

Y

I

L

V

E

G

M

V

P

Q

L

L

S

G

G

K

A

C

T

S

I

V

N

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

N

Q

V

I

Q

N

I

I

A

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

F

Y

I

I

Q

Q

P

S

T

T

C

S

A

D

P

P

A

V

N

N

A

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

T

V

D

D

A

A

L

L

K

K

R

R

S

A

S

S

A

A

K

K

S

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

K

A

S

A

-

P

-

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

D

N

M

L

E

E

K

T

A

A

G

G

I

A

T

T

R

R

V

V

V

I

T

A

V

L

D

D

L

L

H

H

A

A

S

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

L

L

Y

M

G

G

S

V

I

P

L

I

F

L

I

G

D

E

Y

Y

I

F

R

E

S

G

K

K

N

N

F

L

A

E

N

D

P

I

I

V

I

I

A

V

S

S

P

P

D

D

I

H

G

G

V

V

A

T

R

R

A

A

R

R

Y

K

F

L

A

A

S

D

R

R

L

L

G

K

M

A

D

P

M

I

V

A

I

I

V

I

D

D

K

K

R

R

R

-

E

-

K

-

A

-

N

-

V

-

A

-

E

-

V

-

M

M

N

N

I

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

V

A

I

I

L

L

I

I

D

D

M

I

V

I

D

D

T

T

A

A

G

G

T

T

M

I

V

T

K

L

G

A

A

A

A

N

A

A

L

L

K

V

E

E

A

N

G

G

A

A

T

K

S

E

V

V

M

Y

A

A

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

D

E

R

R

I

I

R

N

D

N

G

S

A

T

L

I

D

K

E

E

L

L

V

V

S

T

D

N

T

S

I

I

P

K

L

L

K

P

A

E

E

E

C

K

K

K

-

I

-

E

K

R

I

F

K

K

V

Q

L

L

S

S

T

V

A

G

Q

P

M

L

L

L

G

A

E

E

V

A

I

I

R

I

R

R

V

V

Q

H

N

E

N

Q

E

Q

S

S

V

V

N

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R96 (= R96), Q97 (= Q97), R99 (= R99)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
52% identity, 98% coverage: 3:308/311 of query aligns to 1:297/297 of 1ibsA

query
sites
1ibsA

N

N

Y

L

M

K

L

I

F

F

S

S

G

L

T

N

A

S

N

N

P

P

P

E

L

L

S

A

Q

K

A

E

I

I

A

A

D

D

Y

I

L

V

E

G

M

V

P

Q

L

L

S

G

G

K

A

C

T

S

I

V

N

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

N

Q

V

I

Q

N

I

I

A

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

F

Y

I

I

Q

Q

P

S

T

T

C

S

A

D

P

P

A

V

N

N

A

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

T

V

D

D

A

A

L

L

K

K

R

R

S

A

S

S

A

A

K

K

S

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

K

A

S

A

-

P

-

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

D

N

M

L

E

E

K

T

A

A

G

G

I

A

T

T

R

R

V

V

V

I

T

A

V

L

D

D

L

L

H
|
H

A

A

S

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

L

L

Y

M

G

G

S

V

I

P

L

I

F

L

I

G

D

E

Y

Y

I

F

R

E

S

G

K

K

N

N

F

L

A

E

N

D

P

I

I

V

I

I

A

V

S

S

P

P

D

D

I

H

G

G

V

V

A

T

R

R

A

A

R

R

Y

K

F

L

A

A

S

D

R

R

L

L

G

K

M

A

D

P

M

I

V

A

I

I

V

I

D

D

K

K

R

R

R

-

E

-

K

-

A

-

N

-

V

-

A

-

E

-

V

-

M

M

N

N

I

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

V

A

I

I

L

L

I

I

D

D

M

I

V

I

D
|
D

T
|
T

A
|
A

G

G

T
|
T

M

I

V

T

K

L

G

A

A

A

A

N

A

A

L

L

K

V

E

E

A

N

G

G

A

A

T

K

S

E

V

V

M

Y

A

A

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

D

E

R

R

I

I

R

N

D

N

G

S

A

T

L

I

D

K

E

E

L

L

V

V

S

T

D

N

T

S

I

I

P

K

L

L

K

P

A

E

E

E

C

K

K

K

-

I

-

E

K

R

I

F

K

K

V

Q

L

L

S

S

T

V

A

G

Q

P

M

L

L

L

G

A

E

E

V

A

I

I

R

I

R

R

V

V

Q

H

N

E

N

Q

E

Q

S

S

V

V

N

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R94 (= R96), Q95 (= Q97), R97 (= R99), H126 (= H130)
binding sulfate ion: D209 (= D222), T210 (= T223), A211 (= A224), T213 (= T226)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
48% identity, 99% coverage: 1:309/311 of query aligns to 1:314/318 of P60891

query
sites
P60891

M
|
M

S

P

N

N

Y

I

M

K

L

I

F

F

S

S

G

G

T

S

A

S

N

H

P

Q

P

D

L

L

S
|
S

Q

Q

A

K

I

I

A

A

D

D

Y

R

L

L

E

G

M

L

P

E

L

L

S

G

G

K

A

V

T

V

I

T

N

K

R

K

F

F

S

S

D

N

G

Q

E

E

I

T

N

C

V

V

Q

E

I

I

A

G

E

E

S

S

V

V

R

R

G

G

K

E

D
|
D

V

V

F

Y

I

I

I

V

Q

Q

P

S

T

G

C

C

A

G

P

E

A

I

N

N

A

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

K

R

I

S

A

S

S

A

A

K

S

S

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

K

K

A

D

A

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

D
x
N

M

M

M

L

E

S

K

V

A

A

G

G

I

A

T

D

R

H

V

I

T

T

V

M

D

D

L
|
L

H

H

A

A

S
|
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V
|
V

D

D

N
|
N

L

L

Y
|
Y

G

A

S

E

I

P

L

A

F

V

I

L

D

K

Y

W

I

I

R

R

S

E

K

N

-

I

-

S

N

E

F

W

A

R

N

N

P

C

I

T

I

I

A

V

S

S

P

P

D

D

I

A

G

G

V

A

A

K

R

R

A

V

R

T

Y

S

F

I

A

A

S
x
D

R

R

L

L

G

N

M

V

D

D

M

F

V
x
A

I

L

V

I

D
x
H

K

K

R

E

R

R

E

K

K

K

A

A

N

N

V

E

A

V

E
x
D

V

R

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

V

A

I

I

L

L

I
x
V

D

D

M

M

V

A

D

D

T

T

A

C

G

G

T

T

M

I

V

C

K
x
H

G

A

A

A

A

D

A

K

L

L

K

L

E

S

A

A

G

G

A

A

T

T

S

R

V

V

M

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

D

S

R

R

I

I

R

N

D

N

G

A

A

C

L

F

D

E

E

A

L

V

V

V

S

T

D

N

T

T

I

I

P

P

L

Q

K

E

-

D

-

K

-

M

A

K

E

H

C

C

K

S

K

K

I

I

K

Q

V

V

L

I

S

D

T

I

A

S

Q

M

M

I

L

L

G

A

E

E

V

A

I

I

R

R

V

T

Q

H

N

N

N

G

E

E

S

S

V

V

N

S

S

Y

L

L

F

F

A

S

M1 (= M1) modified: Initiator methionine, Removed
S16 (= S16) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D52) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ D114) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L129) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (= S132) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V142) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N144) mutation to H: No effect on catalytic activity.
Y146 (= Y146) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ S181) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (≠ V188) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D191) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E201) to H: in a breast cancer sample; somatic mutation
V219 (≠ I217) to G: in a breast cancer sample; somatic mutation
H231 (≠ K229) to D: in a colorectal cancer sample; somatic mutation

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
48% identity, 99% coverage: 3:309/311 of query aligns to 2:313/316 of 8dbkB

query
sites
8dbkB

N

N

Y

I

M

K

L

I

F

F

S

S

G

G

T

S

A

S

N

H

P

Q

P

D

L

L

S

S

Q

Q

A

K

I

I

A

A

D

D

Y

R

L

L

E

G

M

L

P

E

L

L

S

G

G

K

A

V

T

V

I

T

N

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

N

C

V

V

Q

E

I

I

A

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

P

S

T

G

C

C

A

G

P

E

A

I

N

N

A

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

K

R

I

S

A

S

S

A

A

K

S

S

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

K

K

A

D

A

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

D

N

M

M

M

L

E

S

K

V

A

A

G

G

I

A

T

D

R

H

V

I

T

T

V

M

D

D

L

L

H
|
H

A

A

S

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

L

Y

Y

G

A

S

E

I

P

L

A

F

V

I

L

D

K

Y

W

I

I

R

R

S

E

K

N

-

I

-

S

N

E

F

W

A

R

N

N

P

C

I

T

I

I

A

V

S

S

P

P

D
|
D

I

A

G
|
G

G

G

V

A

A

K

R

R

A

V

R

T

Y

S

F

I

A

A

S

D

R

R

L

L

G

N

M

V

D

D

M

F

V

A

I

L

V

I

D

H

K
|
K

R

E

R
|
R

E

K

K

K

A

A

N
|
N

V

E

A

V

E

D

V

R

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

V

A

I

I

L

L

I

V

D
|
D

M

M

V

A

D
|
D

T
|
T

A
x
C

G
|
G

T
|
T

M

I

V

C

K

H

G

A

A

A

A

D

A

K

L

L

K

L

E

S

A

A

G

G

A

A

T

T

S

R

V

V

M

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

D

S

R

R

I

I

R

N

D

N

G

A

A

C

L

F

D

E

E

A

L

V

V

V

S

T

D

N

T

T

I

I

P

P

L

Q

K

E

-

D

-

K

-

M

A

K

E

H

C

C

K

S

K

K

I

I

K

Q

V

V

L

I

S

D

T

I

A

S

Q

M

M

I

L

L

G

A

E

E

V

A

I

I

R

R

V

T

Q

H

N

N

N

G

E

E

S

S

V

V

N

S

S

Y

L

L

F

F

A

S

binding adenosine monophosphate: R95 (= R96), Q96 (= Q97), N199 (= N198)
binding adenosine-5'-triphosphate: F34 (= F35), N36 (≠ D37), E38 (= E39)
binding phosphate ion: S46 (= S47), R48 (= R49)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H130), D170 (= D169), G172 (= G171), K193 (= K192), R195 (= R194), D219 (= D218), D220 (= D219), D223 (= D222), T224 (= T223), C225 (≠ A224), G226 (= G225), T227 (= T226)

8dbeA Human prps1 with adp; hexamer (see paper)
48% identity, 99% coverage: 3:309/311 of query aligns to 2:313/316 of 8dbeA

query
sites
8dbeA

N

N

Y

I

M

K

L

I

F

F

S

S

G

G

T

S

A

S

N

H

P

Q

P

D

L

L

S

S

Q

Q

A

K

I

I

A

A

D

D

Y

R

L

L

E

G

M

L

P

E

L

L

S

G

G

K

A

V

T

V

I

T

N

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

N

C

V

V

Q

E

I

I

A

G

E

E

S

S

V

V

R

R

G

G

K

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

P

S

T

G

C

C

A

G

P

E

A

I

N

N

A

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

K

R

I

S

A

S

S

A

A

K

S

S

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K
|
K

A
x
D

A

K

P
x
S

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

D

N

M

M

M

L

E

S

K

V

A

A

G

G

I

A

T

D

R

H

V

I

T

T

V

M

D

D

L

L

H
|
H

A

A

S

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

L

L

Y
|
Y

G

A

S

E

I

P

L

A

F

V

I

L

D

K

Y

W

I

I

R

R

S

E

K

N

-

I

-

S

N

E

F

W

A

R

N

N

P

C

I

T

I

I

A

V

S

S

P

P

D
|
D

I

A

G

G

V

A

A

K

R

R

A

V

R

T

Y

S

F

I

A

A

S

D

R

R

L

L

G

N

M

V

D

D

M

F

V

A

I

L

V

I

D

H

K

K

R

E

R

R

E

K

K

K

A

A

N

N

V

E

A

V

E

D

V

R

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

V

A

I

I

L

L

I

V

D
|
D

M

M

V

A

D
|
D

T
|
T

A

C

G
|
G

T
|
T

M

I

V

C

K

H

G

A

A

A

A

D

A

K

L

L

K

L

E

S

A

A

G

G

A

A

T

T

S

R

V

V

M

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

D

S

R

R

I

I

R

N

D

N

G

A

A

C

L

F

D

E

E

A

L

V

V

V

S

T

D

N

T

T

I

I

P

P

L

Q

K

E

-

D

-

K

-

M

A

K

E

H

C

C

K

S

K

K

I

I

K

Q

V

V

L

I

S

D

T

I

A

S

Q

M

M

I

L

L

G

A

E

E

V

A

I

I

R

R

V

T

Q

H

N

N

N

G

E

E

S
|
S

V
|
V

N
x
S

S

Y

L

L

F
|
F

A

S

binding adenosine-5'-diphosphate: F34 (= F35), N36 (≠ D37), E38 (= E39), R95 (= R96), Q96 (= Q97), K98 (≠ R99), K99 (= K100), D100 (≠ A101), S102 (≠ P103), R103 (= R104), H129 (= H130), D142 (= D143), Y145 (= Y146), S307 (= S303), V308 (= V304), S309 (≠ N305), F312 (= F308)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H130), D170 (= D169), D219 (= D218), D220 (= D219), D223 (= D222), T224 (= T223), G226 (= G225), T227 (= T226)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
48% identity, 98% coverage: 3:308/311 of query aligns to 1:305/305 of 2hcrA

query
sites
2hcrA

N

N

Y

I

M

K

L

I

F

F

S

S

G

G

T

S

A

S

N

H

P

Q

P

D

L

L

S

S

Q

Q

A

K

I

I

A

A

D

D

Y

R

L

L

E

G

M

L

P

E

L

L

S

G

G

K

A

V

T

V

I

T

N

K

R

K

F

F

S

S

D

N

G

Q

E

E

I

T

N

C

V

V

Q

E

I

I

A

G

E

E

S

S

V

V

R

R

G

G

K

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

P

S

T

G

C

C

A

G

P

E

A

I

N

N

A

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

K

R

I

S

A

S

S

A

A

K

S

S

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K

K

A

D

A

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

D

N

M

M

M

L

E

S

K

V

A

A

G

G

I

A

T

D

R

H

V

I

T

T

V

M

D

D

L

L

H
|
H

A

A

S

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

L

Y

Y

G

A

S

E

I

P

L

A

F

V

I

L

D

K

Y

W

I

I

R

R

S

E

K

N

-

I

-

S

N

E

F

W

A

R

N

N

P

C

I

T

I

I

A

V

S

S

P

P

D

D

I

A

G

G

V

A

A

K

R

R

A

V

R

T

Y

S

F

I

A

A

S

D

R

R

L

L

G

N

M

V

D

D

M

F

V

A

I

L

V

I

D

H

K

K

R

E

R

R

E

D

K

R

A

-

N

-

V

-

A

-

E

-

V

-

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

V

A

I

I

L

L

I

V

D

D

M

M

V

A

D

D

T

T

A

C

G

G

T

T

M

I

V

C

K

H

G

A

A

A

A

D

A

K

L

L

K

L

E

S

A

A

G

G

A

A

T

T

S

R

V

V

M

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

D

S

R

R

I

I

R

N

D

N

G

A

A

C

L

F

D

E

E

A

L

V

V

V

S

T

D

N

T

T

I

I

P

P

L

Q

K

E

-

D

-

K

-

M

A

K

E

H

C

C

K

S

K

K

I

I

K

Q

V

V

L

I

S

D

T

I

A

S

Q

M

M

I

L

L

G

A

E

E

V

A

I

I

R

R

V

T

Q

H

N

N

N

G

E

E

S

S

V

V

N

S

S

Y

L

L

F

F

binding adenosine monophosphate: R94 (= R96), Q95 (= Q97), K97 (≠ R99), H128 (= H130)

7yk1A Structural basis of human prps2 filaments (see paper)
47% identity, 99% coverage: 3:309/311 of query aligns to 2:304/306 of 7yk1A

query
sites
7yk1A

N

N

Y

I

M

V

L

L

F

F

S

S

G

G

T

S

A

S

N

H

P

Q

P

D

L

L

S

S

Q

Q

A

R

I

V

A

A

D

D

Y

R

L

L

E

G

M

L

P

E

L

L

S

G

G

K

A

V

T

V

I

T

N

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

N

S

V

V

Q

E

I

I

A

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

V

V

F

Y

I

I

Q

Q

P

S

T

G

C

C

A

G

P

E

A

I

N

N

A

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

K

R

I

S

A

S

S

A

S

K

S

S

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q

Q

D

D

R

K

K
|
K

A
x
D

A
x
K

P
x
S

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

D

N

M

M

M

L

E

S

K

V

A

A

G

G

I

A

T

D

R

H

V

I

T

T

V

M

D

D

L

L

H
|
H

A

A

S

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

L

L

Y

Y

G

A

S

E

I

P

L

A

F

V

I

L

D

Q

Y

W

I

I

R

R

S

E

K

N

-

I

-

A

N

E

F

W

A

K

N

N

P

C

I

I

A

V

S

S

P

P

D

D

I

A

G

G

V

A

A

K

R

R

A

V

R

T

Y

S

F

I

A

A

S

D

R

R

L

L

G

N

M

V

D

E

M

F

V

A

I

L

V

I

D

H

K

K

R

E

R

-

E

-

K

-

A

-

N

-

V

-

A

-

E

-

V

-

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

V

A

I

I

L

L

I

V

D

D

M

M

V

A

D
|
D

T

T

A
x
C

G

G

T
|
T

M

I

V

C

K

H

G

A

A

A

A

D

A

K

L

L

K

L

E

S

A

A

G

G

A

A

T

T

S

K

V

V

M

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

D

S

R

R

I

I

R

N

D

N

G

A

A

A

L

F

D

E

E

A

L

V

V

V

S

T

D

N

T

T

I

I

P

P

L

Q

K

E

-

D

-

K

-

M

A

K

E

H

C

C

K

T

K

K

I

I

K

Q

V

V

L

I

S

D

T

I

A

S

Q

M

M

I

L

L

G

A

E

E

V

A

I

I

R

R

V

T

Q

H

N

N

N

G

E

E

S
|
S

V

V

N
x
S

S

Y

L

L

F
|
F

A

S

binding adenosine-5'-diphosphate: F34 (= F35), N36 (≠ D37), E38 (= E39), S46 (= S47), R48 (= R49), R95 (= R96), K99 (= K100), D100 (≠ A101), K101 (≠ A102), S102 (≠ P103), R103 (= R104), H129 (= H130), D142 (= D143), S298 (= S303), S300 (≠ N305), F303 (= F308)
binding phosphate ion: D214 (= D222), C216 (≠ A224), T218 (= T226)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
47% identity, 99% coverage: 3:309/311 of query aligns to 2:306/309 of 8dbgA

query
sites
8dbgA

N

N

Y

I

M

K

L

I

F

F

S

S

G

G

T

S

A

S

N

H

P

Q

P

D

L

L

S

S

Q

Q

A

K

I

I

A

A

D

D

Y

R

L

L

E

G

M

L

P

E

L

L

S

G

G

K

A

V

T

V

I

T

N

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

N

C

V

V

Q

E

I

I

A

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

P

S

T

G

C

C

A

G

P

E

A

I

N

N

A

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

K

R

I

S

A

S

S

A

A

K

S

S

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K

K

A

D

A

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

D

N

M

M

M

L

E

S

K

V

A

A

G

G

I

A

T

D

R

H

V

I

T

T

V

M

D

D

L

L

H
|
H

A

A

S

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

L

Y

Y

G

A

S

E

I

P

L

A

F

V

I

L

D

K

Y

W

I

I

R

R

S

E

K

N

-

I

-

S

N

E

F

W

A

R

N

N

P

C

I

T

I

I

A

V

S

S

P

P

D

D

I

A

G

G

V

A

A

K

R

R

A

V

R

T

Y

S

F

I

A

A

S

D

R

R

L

L

G

N

M

V

D

D

M

F

V

A

I

L

V

I

D

H

K

K

R

E

R

-

E

-

K

-

A

-

N

-

V

-

A

-

E

D

V

R

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

V

A

I

I

L

L

I

V

D

D

M

M

V

A

D
|
D

T
|
T

A
x
C

G

G

T
|
T

M

I

V

C

K

H

G

A

A

A

A

D

A

K

L

L

K

L

E

S

A

A

G

G

A

A

T

T

S

R

V

V

M

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

D

S

R

R

I

I

R

N

D

N

G

A

A

C

L

F

D

E

E

A

L

V

V

V

S

T

D

N

T

T

I

I

P

P

L

Q

K

E

-

D

-

K

-

M

A

K

E

H

C

C

K

S

K

K

I

I

K

Q

V

V

L

I

S

D

T

I

A

S

Q

M

M

I

L

L

G

A

E

E

V

A

I

I

R

R

V

T

Q

H

N

N

N

G

E

E

S

S

V

V

N

S

S

Y

L

L

F

F

A

S

binding adenosine-5'-triphosphate: F34 (= F35), N36 (≠ D37), E38 (= E39), R95 (= R96), Q96 (= Q97), K98 (≠ R99), H129 (= H130)
binding phosphate ion: S46 (= S47), R48 (= R49), D216 (= D222), T217 (= T223), C218 (≠ A224), T220 (= T226)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
45% identity, 98% coverage: 6:309/311 of query aligns to 8:317/321 of O94413

query
sites
O94413

L

I

F

F

S

A

G

G

T

N

A

S

N

H

P

P

P

E

L

L

S

A

Q

E

A

K

I

V

A

A

D

R

Y

R

L

I

E

G

M

L

P

S

L

L

S

G

G

K

A

V

T

A

I

V

N

V

R

Q

F

Y

S

S

D

N

G

R

E

E

I

T

N

S

V

V

Q

T

I

I

A

G

E

E

S

S

V

V

R

R

G

D

K

E

D

D

V

V

F

F

I

I

I

L

Q

Q

P

T

T

G

C

C

A

G

P

S

A

I

N

N

A

D

N

H

L

L

M

M

E

E

L

L

I

I

M

M

T

I

D

N

A

A

L

C

K

R

R

S

S

A

S

S

A

A

K

R

S

R

I

I

T

T

A

A

V

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

K

K

A

D

A

K

P

S

R

R

V

A

P

P

I

I

T

T

A

A

K

R

L

L

V

V

A

A

D

N

M

M

M

L

E

Q

K

T

A

A

G

G

I

C

T

N

R

H

V

I

T

T

V

M

D

D

L

L

H

H

A

A

S

S

Q

Q

I

I

Q

Q

G

G

F

F

D

N

I

V

P

P

V

V

D

D

N

N

L

L

Y

Y

G

A

S

E

I

P

L

S

F

V

I

L

D

R

Y

Y

I

I

R

R

S

E

K

N

-

I

-

D

N

T

F

T

A

V

N

N

P

P

-

T

I

V

I

I

A

V

S
|
S

P

P

D

D

I

A

G

G

V

A

A

K

R

R

A

A

R

T

Y

A

F

L

A

A

S

D

R

R

L

L

G

D

M

L

D

D

M

F

V

A

I

L

V

I

D

H

K

K

R

E

R

R

E

Q

K

K

A

A

N

N

V

E

A

V

E

S

V

R

M

M

N

V

I

L

I

V

G

G

D

D

V

V

R

R

G

D

R

K

D

L

V

A

I

I

L

L

I

V

D

D

M

M

V

A

D

D

T

T

A

C

G

G

T

T

M

L

V

G

K

L

G

A

A

A

A

K

A

T

L

L

K

K

E

D

A

N

G

G

A

A

T

K

S

A

V

V

M

Y

A

A

C

I

C

V

T

T

H

H

P

G

V

I

L

L

S

S

G

G

P

K

A

A

I

I

D

K

R

V

I

I

R

N

D

E

G

S

A

A

L

L

D

E

E

K

L

V

V

I

V

V

S

T

D

N

T

T

I

I

P

P

L

H

-

D

-

K

K

R

A

S

E

L

C

C

K

S

K

K

I

I

K

E

V

T

L

I

S

D

T

I

A

S

Q

G

M

V

L

L

G

A

E

E

V

C

I

I

R

R

V

I

Q

H

N

H

N

G

E

E

S

S

V

V

N

S

S

V

L

L

F

F

A

S

S172 (= S167) modified: Phosphoserine

Query Sequence

>WP_013554179.1 NCBI__GCF_000186245.1:WP_013554179.1
MSNYMLFSGTANPPLSQAIADYLEMPLSGATINRFSDGEINVQIAESVRGKDVFIIQPTC
APANANLMELLIMTDALKRSSAKSITAVVPYYGYARQDRKAAPRVPITAKLVADMMEKAG
ITRVVTVDLHASQIQGFFDIPVDNLYGSILFIDYIRSKNFANPIIASPDIGGVARARYFA
SRLGMDMVIVDKRREKANVAEVMNIIGDVRGRDVILIDDMVDTAGTMVKGAAALKEAGAT
SVMACCTHPVLSGPAIDRIRDGALDELVVSDTIPLKAECKKIKVLSTAQMLGEVIRRVQN
NESVNSLFATT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory