SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013554780.1 NCBI__GCF_000186245.1:WP_013554780.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

Q5F8J4 NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
44% identity, 95% coverage: 20:419/420 of query aligns to 22:431/435 of Q5F8J4

query
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Q5F8J4

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L45 (= L45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ LN 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
40% identity, 100% coverage: 2:419/420 of query aligns to 5:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), L12 (≠ V9), G13 (= G10), N14 (≠ T11), V15 (= V12), V45 (= V42), R46 (= R43), R47 (≠ D44), R52 (= R49), I63 (≠ P59), L78 (= L74), M79 (= M75), P84 (≠ K80), A102 (= A98), K104 (= K100), G306 (= G295), T310 (= T299)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
42% identity, 74% coverage: 2:310/420 of query aligns to 5:306/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D194), K194 (= K198)
binding lysine: R215 (≠ E219), G219 (≠ E223)

Sites not aligning to the query:

binding lysine: 394, 395

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
40% identity, 78% coverage: 2:330/420 of query aligns to 4:327/331 of 6a0sA

query
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6a0sA

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V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

Y

E

H

A

R

W

Y

E

E

S

L

L

Q

R

E

P

I

F

A

A

G

E

E

E

I

G

P

L

L

I

F

Y

Y

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

G

S

A

F

L

L

R

E

N

T

G

-

L

L

S

R

A

G

N

S

H

E

I

L

E

L

S

E

I

L

K

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

M

I

L

L

T

Q

K

E

M

M

I

-

Q

E

E

K

G

G

V

R

A

T

Y

Y

E

A

E

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

G

E

G

G

Y

I

D
|
D

A

A

H

H

K
|
K

L

L

L

T

I

L

L

L

G

A

S

R

I

L

A

L

Y

V

G

D

I

P

D

G

A

F

K

P

P

F

E

A

E

E

I

V

I

E

I

A

E

Q

G

G

I

I

E

A

E

R

I

L

S

T

P

P

T

E

D

V

V

L

E

Q

F

K

A

A

R

E

E

A

F

R

G

G

Y

E

E

R

I

V

K

R

L

L

L

V

G

A

I

-

A

S

K

L

K

F

G

G

E

E

K

G

G

G

-

R

V

W

E

R

M

A

R

A

V

V

H

A

A

P

T

R

M

R

I

L

P

P

A

Q

K

D

S

H

M

P

I

L

A

A

K

R

V

A

D

R

G

G

V

-

M

-

N

N

A

A

V

L

S

W

V

V

V

R

G

A

D

R

R

P

V

L

G

G

E

E

T

A

M

F

F

V

Y

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

D

G

A

A

T
|
T

A

A

S

S

A

G

V

L

I

F

A

A

D

D

I

L

V

L

E

R

I

F

V

L

R

S

G

G

N

A

S

P

G

G

-

H

-

L

P

P

M

A

L

P

G

R

Y

A

K

R

K

P

G

P

L

L

E

E

S

E

G

G

L

S

P

P

active site: D191 (= D194), K195 (= K198)
binding l-homoserine: K99 (= K100), N150 (= N152), G151 (= G153), T152 (= T154), Y178 (= Y181), E180 (= E183), D186 (= D189), K195 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ V9), G12 (= G10), T13 (= T11), V14 (= V12), L42 (vs. gap), V43 (= V42), R44 (= R43), D45 (= D44), K48 (= K47), R50 (= R49), A73 (≠ L74), M74 (= M75), A97 (= A98), K99 (= K100), G177 (= G180), E180 (= E183), A289 (= A294), G290 (= G295), T294 (= T299)

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
40% identity, 78% coverage: 2:330/420 of query aligns to 4:327/331 of 2ejwA

query
sites
2ejwA

V

L

K

K

I

I

G

A

I

L

L

L

G

G

V

G

G

G

T

T

V

V

G

G

E

S

S

A

V

F

A

Y

K

N

I

L

L

V

L

L

E

E

N

R

A

A

D

E

I

E

I

L

E

S

A

A

R

F

A

G

G

-

K

-

I

V

V

V

P

P

-

R

V

F

L

L

G

G

A

V

-

L

V

V

R

R

D

D

L

P

N

R

K

K

P

P

R

R

D

-

V

-

S

A

I

I

P

P

-

Q

-

E

-

L

L

L

T

R

D

A

D

E

P

P

G

F

E

D

V

L

I

L

E

E

S

A

A

-

D

-

V

-

D

D

I

L

V

V

E

E

L

A

M

M

G

G

I

V

E

E

K

A

P

P

Y

L

R

R

L

L

V

V

R

L

K

P

A

A

L

L

E

E

A

A

G

G

K

I

P

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

Y

E

H

A

R

W

Y

E

E

S

L

L

Q

R

E

P

I

F

A

A

G

E

E

E

I

G

P

L

L

I

F

Y

Y

H

E
|
E

A

A

S

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

I

A

I

L

G

S

A

F

L

L

R

E

N

T

G

-

L

L

S

R

A

G

N

S

H

E

I

L

E

L

S

E

I

L

K

H

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

L

Y

Y

M

I

L

L

T

Q

K

E

M

M

I

-

Q

E

E

K

G

G

V

R

A

T

Y

Y

E

A

E

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

V

V

G

E

G

G

Y

I

D
|
D

A

A

H

H

K
|
K

L

L

L

T

I

L

L

L

G

A

S

R

I

L

A

L

Y

V

G

D

I

P

D

G

A

F

K

P

P

F

E

A

E

E

I

V

I

E

I

A

E

Q

G

G

I

I

E

A

E

R

I

L

S

T

P

P

T

E

D

V

V

L

E

Q

F

K

A

A

R

E

E

A

F

R

G

G

Y

E

E

R

I

V

K

R

L

L

L

V

G

A

I

-

A

S

K

L

K

F

G

G

E

E

K

G

G

G

-

R

V

W

E

R

M

A

R

A

V

V

H

A

A

P

T

R

M

R

I

L

P

P

A

Q

K

D

S

H

M

P

I

L

A

A

K

R

V

A

D

R

G

G

V

-

M

-

N

N

A

A

V

L

S

W

V

V

V

R

G

A

D

R

R

P

V

L

G

G

E

E

T

A

M

F

F

V

Y

T

G

G

P

P

G

G

A

A

G

G

D

G

A

A

T

T

A

A

S

S

A

G

V

L

I

F

A

A

D

D

I

L

V

L

E

R

I

F

V

L

R

S

G

G

N

A

S

P

G

G

-

H

-

L

P

P

M

A

L

P

G

R

Y

A

K

R

K

P

G

P

L

L

E

E

S

E

G

G

L

S

P

P

active site: D191 (= D194), K195 (= K198)
binding magnesium ion: E121 (= E122), V124 (= V125), M125 (≠ A126), A126 (≠ G127), T128 (≠ I129)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
40% identity, 78% coverage: 2:330/420 of query aligns to 4:327/332 of 6a0tB

query
sites
6a0tB

V

L

K

K

I

I

G

A

I

L

L

L

G
|
G

V
x
G

G
|
G

T
|
T

V
|
V

G

G

E

S

S

A

V

F

A

Y

K

N

I

L

L

V

L

L

E

E

N

R

A

A

D

E

I

E

I

L

E

S

A

A

R

F

A

G

G

-

K

-

I

V

V

V

P

P

-

R

V

F

L

L

G

G

A

V

-
x
L

V
|
V

R
|
R

D
|
D

L

P

N

R

K
|
K

P

P

R
|
R

D

-

V

-

S

A

I

I

P

P

-

Q

-

E

-

L

L

L

T

R

D

A

D

E

P

P

G

F

E

D

V

L

I

L

E

E

S

A

A

-

D

-

V

-

D

D

I

L

V

V

E

E

L
x
A

M
|
M

G
|
G

G

G

I

V

E

E

K

A

P

P

Y

L

R

R

L

L

V

V

R

L

K

P

A

A

L

L

E

E

A

A

G

G

K

I

P

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

Y

E

H

A

R

W

Y

E

E

S

L

L

Q

R

E

P

I

F

A

A

G

E

E

E

I

G

P

L

L

I

F

Y

Y

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

G

S

A

F

L

L

R

E

N

T

G

-

L

L

S

R

A

G

N

S

H

E

I

L

E

L

S

E

I

L

K

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

M

I

L

L

T

Q

K

E

M

M

I

-

Q

E

E

K

G

G

V

R

A

T

Y

Y

E

A

E

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

G

E

G

G

Y

I

D
|
D

A

A

H

H

K
|
K

L

L

L

T

I

L

L

L

G

A

S

R

I

L

A

L

Y

V

G

D

I

P

D

G

A

F

K

P

P

F

E

A

E

E

I

V

I

E

I

A

E

Q

G

G

I

I

E

A

E

R

I

L

S

T

P

P

T

E

D

V

V

L

E

Q

F

K

A

A

R

E

E

A

F

R

G

G

Y

E

E

R

I

V

K

R

L

L

L

V

G

A

I

-

A

S

K

L

K

F

G

G

E

E

K

G

G

G

-

R

V

W

E

R

M

A

R

A

V

V

H

A

A

P

T

R

M

R

I

L

P

P

A

Q

K

D

S

H

M

P

I

L

A

A

K

R

V

A

D

R

G

G

V

-

M

-

N

N

A

A

V

L

S

W

V

V

V

R

G

A

D

R

R

P

V

L

G

G

E

E

T

A

M

F

F

V

Y

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

D

G

A

A

T
|
T

A

A

S

S

A

G

V

L

I

F

A

A

D

D

I

L

V

L

E

R

I

F

V

L

R

S

G

G

N

A

S

P

G

G

-

H

-

L

P

P

M

A

L

P

G

R

Y

A

K

R

K

P

G

P

L

L

E

E

S

E

G

G

L

S

P

P

active site: D191 (= D194), K195 (= K198)
binding l-homoserine: N150 (= N152), G151 (= G153), T152 (= T154), Y178 (= Y181), E180 (= E183), D186 (= D189), K195 (= K198)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G8), G11 (≠ V9), G12 (= G10), T13 (= T11), V14 (= V12), L42 (vs. gap), V43 (= V42), R44 (= R43), D45 (= D44), K48 (= K47), R50 (= R49), A73 (≠ L74), M74 (= M75), G75 (= G76), A97 (= A98), N98 (= N99), G177 (= G180), E180 (= E183), A289 (= A294), G290 (= G295), T294 (= T299)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 73% coverage: 2:309/420 of query aligns to 3:310/319 of 4xb1A

query
sites
4xb1A

V

V

K

N

I

I

G

S

I

I

L
x
F

G

G

V
x
F

G
|
G

T
|
T

V
|
V

G

G

E

R

S

A

V

L

A

A

K

E

I

I

L

I

L

A

E

E

N

K

A

S

-

R

-

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

-

S

-

I

-

T

-
x
D

-
x
R

-

S

-

G

-

T

-

I

-

W

-

G

-

D

-

F

D

D

I

L

E

E

A

A

R

K

A

E

G

V

K
|
K

K

E

I

S

V

T

P

G

V

K

L

L

G

S

A

N

V

I

R

G

D

D

L

Y

N

E

K

-

P

-

R

-

D

-

V

-

S

-

I

-

P

V

L

Y

T

N

D

F

D

S

P

P

G

Q

E

E

V

L

I

V

E

E

S

E

A

V

D

K

V

P

D

N

I

I

V

L

V

V

E

D

L
x
V

M

-

G
x
S

I

W

E

D

K

E

P

A

Y

H

R

E

L

M

V

Y

R

K

K

V

A

A

L

L

E

G

A

E

G

G

K

I

P

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

Y

N

H

Y

R

Y

Y

D

E

E

L

L

Q

M

E

N

I

L

A

A

G

K

E

E

I

N

P

N

-

A

-

G

L

I

F

F

Y

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

G

G

A

V

L

L

R

R

N

E

G

N

L

L

S

L

A

G

N

E

H

N

I

I

E

K

S

R

I

I

K

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

M

I

L

L

T

T

K

K

M

M

I

-

Q

S

E

E

G

G

V

K

A

T

Y

L

E

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

V

I

G

D

G

G

Y

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

L

K

I

I

L

L

G

H

S

W

I

V

A

S

Y

Y

G

G

I

-

D

-

A

E

K

P

P

P

E

E

I

E

I

R

E

L

G

G

I

I

E

R

E

E

I

V

S

R

P

D

T

-

D

-

V

-

E

-

F

-

A

A

R

R

E

-

F

-

G

-

Y

-

E

N

I

V

K

R

L

L

L

V

G

A

I

Q

A

V

K

S

K

K

G

G

E

K

K

I

G

S

V

V

E

K

M

P

R

R

V

K

H

L

A

S

T

S

M

D

I

N

P

P

A

-

K

-

S

-

M

-

I

-

A

L

K

L

V

V

D

E

G

G

V

V

M

Q

N

N

A

A

V

A

S

V

V

I

V

R

G

T

D

N

R

N

V

L

G

G

E

E

T

V

M

I

F

L

Y

K

G

G

P

P

G

G

A
x
G

G

G

D

R

A

V

T
|
T

A

A

S

S

A

G

V

V

I

F

A

T

D

D

I

I

V

I

E

K

active site: D211 (= D194), K215 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L7), F10 (≠ V9), G11 (= G10), T12 (= T11), V13 (= V12), D39 (vs. gap), R40 (vs. gap), K57 (= K33), V91 (≠ L74), S92 (≠ G76), S93 (≠ G77), S114 (≠ A98), K116 (= K100), S141 (≠ A123), G295 (≠ A294), T300 (= T299)

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 73% coverage: 2:309/420 of query aligns to 3:310/319 of 4xb2A

query
sites
4xb2A

V

V

K

N

I

I

G

S

I

I

L
x
F

G

G

V
x
F

G
|
G

T
|
T

V
|
V

G

G

E

R

S

A

V

L

A

A

K

E

I

I

L

I

L

A

E

E

N

K

A

S

D

R

I

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

-

S

I

I

E

T

A

D

R
|
R

A

S

G

G

-

T

-

I

-

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

K

K

K

E

I

V

V

A

P

E

V

S

L

T

G

G

A

K

V

L

R

S

D

N

L

I

N

G

K

D

P

Y

R

E

D

V

V

Y

S

N

I

F

P

-

L

-

T

-

D

-

D

S

P

P

G

Q

E

E

V

L

I

V

E

E

S

E

A

V

D

K

V

P

D

N

I

I

V

L

V

V

E

D

L
x
V

M

-

G
x
S

I

W

E

D

K

E

P

A

Y

H

R

E

L

M

V

Y

R

K

K

V

A

A

L

L

E

G

A

E

G

G

K

I

P

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

Y

N

H

Y

R

Y

Y

D

E

E

L

L

Q

M

E

N

I

L

A

A

G

K

E

E

I

N

P

N

-

A

-

G

L

I

F

F

Y

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

G

G

A

V

L

L

R

R

N

E

G

N

L

L

S

L

A

G

N

E

H

N

I

I

E

K

S

R

I

I

K

D

G

A

I

V

M

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

M

I

L

L

T

T

K

K

M

M

I

-

Q

S

E

E

G

G

V

K

A

T

Y

L

E

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

V

I

G

D

G

G

Y

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

L

K

I

I

L

L

G

H

S

W

I

V

A

S

Y

Y

G

G

I

-

D

-

A

E

K

P

P

P

E

E

I

E

I

R

E

L

G

G

I

I

E

R

E

E

I

V

S

R

P

D

T

-

D

-

V

-

E

-

F

-

A

A

R

R

E

-

F

-

G

-

Y

-

E

N

I

V

K

R

L

L

L

V

G

A

I

Q

A

V

K

S

K

K

G

G

E

K

K

I

G

S

V

V

E

K

M

P

R

R

V

K

H

L

A

S

T

S

M

D

I

N

P

P

A

-

K

-

S

-

M

-

I

-

A

L

K

L

V

V

D

E

G

G

V

V

M

Q

N

N

A

A

V

A

S

V

V

I

V

R

G

T

D

N

R

N

V

L

G

G

E

E

T

V

M

I

F

L

Y

K

G

G

P

P

G

G

A
x
G

G

G

D

R

A

V

T
|
T

A

A

S

S

A

G

V

V

I

F

A

T

D

D

I

I

V

I

E

K

active site: D211 (= D194), K215 (= K198)
binding l-homoserine: A171 (≠ G153), S172 (≠ T154), D206 (= D189), K215 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L7), F10 (≠ V9), G11 (= G10), T12 (= T11), V13 (= V12), R40 (= R30), V91 (≠ L74), S92 (≠ G76), S93 (≠ G77), S114 (≠ A98), N115 (= N99), K116 (= K100), S141 (≠ A123), G295 (≠ A294), T300 (= T299)

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
31% identity, 74% coverage: 2:312/420 of query aligns to 1:297/300 of 7f4cA

query
sites
7f4cA

V

M

K

K

I

L

G

L

I

L

L
x
F

G
|
G

V
x
Y

G
|
G

T
x
N

V
|
V

G

G

E

K

S

A

V

F

A

R

K

K

I

L

L

L

L

H

E

E

N

K

-

R

-

S

-

P

-

E

-

L

A

N

D

D

I

V

I

I

E

I

A

G

R

-

A

-

G

-

K

-

K

G

I

I

V

V

P
x
T

V
x
R

L
x
R

G

G

A

I

V

M

R

-

D

-

L

L

N

Q

K

D

P

K

R

E

D

D

V

F

S

T

I

P

P

D

L

L

T

E

D

G

D

D

P

V

G

F

E

K

V

A

I

F

E

E

S

K

A

I

D

K

V

P

D

D

I

I

V

I

V

V

E

D

L
x
V

M
x
S

G
x
S

G

A

I

N

-

Y

-

N

-

G

E

E

K

P

P

S

Y

L

R

S

L

L

V

Y

R

K

K

E

A

A

L

I

E

K

A

D

G

G

K

V

P

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

Y

L

H

A

R

F

Y

N

E

E

L

I

Q

F

E

S

I

L

A

A

G

R

E

S

-

K

-

G

I

V

P

K

L

I

F

G

Y

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

G

N

A

L

L

Y

R

R

N

-

G

V

L

L

S

P

A

G

N

S

H

R

I

V

E

I

S

K

I

I

K

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

M

I

L

L

T

T

K

-

M

L

I

M

Q

N

E

K

G

G

V

V

A

S

Y

F

E

E

V

A

L

L

K

K

E

E

A

A

Q

Q

E

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

V

I

G

N

G

G

Y

F

D

D

A

A

H

A

K

K

L

I

L

T

I

I

L

L

G

A

S

N

I

F

A

M

Y

I

G

G

I

N

D

S

A

V

K

T

P

I

E

K

E

D

I

V

I

K

I

F

E

E

G

G

I

I

E

N

E

R

I

-

S

-

P

-

T

-

D

-

V

-

E

-

F

-

A

-

R

-

E

-

F

-

G

-

Y

-

E

D

I

L

K

K

L

I

L

K

G

L

I

I

A

A

K

Y

K

A

G

D

E

E

K

K

G

-

V

-

E

E

M

V

R

W

V

V

H

K

A

P

T

L

M

P

I

I

P

S

A

Q

K

D

S

D

M

P

I

L

A

Y

K

N

V

V

D

D

G

G

V

V

M

E

N

N

A

A

V

L

S

E

V

I

V

T

G

T

D

D

R

-

V

I

G

Q

E

S

T

I

M

L

F

I

Y

R

G

G

P

P

G

G

A

A

G
|
G

G

P

D

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

I

L

A

S

D

D

I

L

V

I

E

L

I

L

V

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ L7), G7 (= G8), Y8 (≠ V9), G9 (= G10), N10 (≠ T11), V11 (= V12), T37 (≠ P37), R38 (≠ V38), R39 (≠ L39), V72 (≠ L74), S73 (≠ M75), S74 (≠ G76), T100 (≠ A98), K102 (= K100), G127 (≠ A123), S131 (≠ G127), E185 (= E183), G280 (= G295), A284 (≠ T299)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
30% identity, 74% coverage: 2:312/420 of query aligns to 1:299/302 of 5x9dA

query
sites
5x9dA

V

M

K

K

I

L

G

L

I

L

L
x
F

G
|
G

V
x
Y

G
|
G

T
x
N

V
|
V

G

G

E

K

S

A

V

F

A

R

K

K

I

L

L

L

L

H

E

E

N

K

-

R

-

S

-

P

-

E

-

L

A

N

D

D

I

V

I

I

E

I

A

G

R

-

A

-

G

-

K

-

K

G

I

I

V

V

P
x
T

V
x
R

L

R

G

G

A

I

V

M

R

-

D

-

L

L

N

Q

K

D

P

K

R

E

D

D

V

F

S

T

I

P

P

D

L

L

T

E

D

G

D

D

P

V

G

F

E

K

V

A

I

F

E

E

S

K

A

I

D

K

V

P

D

D

I

I

V

I

V

V

E

D

L
x
V

M
x
S

G
x
S

G

A

I

N

-

Y

-

N

-

G

E

E

K
x
P

P

S

Y

L

R

S

L

L

V

Y

R

K

K

E

A

A

L

I

E

K

A

D

G

G

K

V

P

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

Y

L

H

A

R

F

Y

N

E

E

L

I

Q

F

E

S

I

L

A

A

G

R

E

S

-

K

-

G

I

V

P

K

L

I

F

G

Y

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

G

N

A

L

L

Y

R

R

N

-

G

V

L

L

S

P

A

G

N

S

H

R

I

V

E

I

S

K

I

I

K

R

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

M

I

L

L

T

T

K

-

M

L

I

M

Q

N

E

K

G

G

V

V

A

S

Y

F

E

E

V

A

L

L

K

K

E

E

A

A

Q

Q

E

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

V

I

G

N

G

G

Y

F

D
|
D

A

A

H

A

K
|
K

L

I

L

T

I

I

L

L

G

A

S

N

I

F

A

M

Y

I

G

G

I

N

D

S

A

V

K

T

P

I

E

K

E

D

I

V

I

K

I

F

E

E

G

G

I

I

E

N

E

R

I

D

S

P

P

K

T

N

D

E

V

-

E

-

F

-

A

-

R

-

E

-

F

-

G

-

Y

-

E

K

I

I

K

K

L

L

L

I

G

A

I

Y

A

A

K

D

K

E

G

K

E

E

K

-

G

-

V

-

E

-

M

-

R

-

V

V

H

W

A

V

T

K

M

P

I

L

P

P

-

I

A

S

K

Q

S

D

M

P

I

L

A

Y

K

N

V

V

D

D

G

G

V

V

M

E

N

N

A

A

V

L

S

E

V

I

V

T

G

T

D

D

R

-

V

I

G

Q

E

S

T

I

M

L

F

I

Y

R

G

G

P

P

G

G

A
|
A

G
|
G

G

P

D

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

I

L

A

S

D

D

I

L

V

I

E

L

I

L

V

K

R

R

active site: D196 (= D194), K200 (= K198)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ L7), G7 (= G8), Y8 (≠ V9), G9 (= G10), N10 (≠ T11), V11 (= V12), T37 (≠ P37), R38 (≠ V38), V72 (≠ L74), S73 (≠ M75), S74 (≠ G76), P82 (≠ K80), T100 (≠ A98), N101 (= N99), K102 (= K100), G127 (≠ A123), S131 (≠ G127), N155 (= N152), G156 (= G153), T157 (= T154), Y183 (= Y181), A184 (= A182), E185 (= E183), D191 (= D189), D196 (= D194), K200 (= K198), A281 (= A294), G282 (= G295), A286 (≠ T299)

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
33% identity, 35% coverage: 80:225/420 of query aligns to 94:240/319 of 3ingA

query
sites
3ingA

K

R

P

E

Y

Y

R

S

L

L

V

Y

R

R

K

M

A

A

L

F

E

E

A

S

G

G

K

M

P

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

Y

N

H

K

R

W

Y

H

E

D

L

I

Q

M

E

D

I

S

A

A

G

N

E

Q

I

N

P

S

L

K

F

Y

-

I

-

R

Y

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

G

S

A

V

L

L

R

D

N

Y

G

S

L

I

S

L

A

P

N

S

H

K

I

V

E

K

S

R

I

F

K

R

G

G

I

I

M

V

N

S

G

S

T

T

C

I

N

N

Y

Y

M

V

L

I

T

R

K

N

M

M

I

-

Q

A

E

N

G

G

V

R

A

S

Y

L

E

R

E

D

V

V

L

V

K

D

E

D

A

A

Q

I

E

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

V

L

G

N

G

G

Y

L

D
|
D

A

A

H

R

K
|
K

L

S

L

V

I

I

L

L

G

V

S

N

I

H

A

I

Y

F

G

G

I

T

D

E

A

Y

K

T

P

L

E

N

E

D

I

V

I

E

I

Y

E

S

G

G

I

V

E

D

E

E

I

R

S

S

active site: D209 (= D194), K213 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A112 (= A98), N113 (= N99), K114 (= K100), A139 (= A123), E198 (= E183), S199 (≠ A184)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 38, 39, 57, 85, 86, 87, 302, 305

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 55% coverage: 82:310/420 of query aligns to 659:908/916 of O81852

query
sites
O81852

Y

Y

R

D

L

W

V

L

R

R

K

K

A

G

L

I

E

H

A

V

G

I

K

T

P

P

V

N

V

K

T

K

A

A

N

N

K

S

A

G

L

P

L

L

-

D

A

Q

Y

Y

H

L

R

K

Y

L

E

R

L

D

Q

L

E

Q

I

R

A

K

G

S

E

Y

I

T

P

H

L

Y

F

F

Y

Y

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

G

S

A

T

L

L

R

R

N

G

G

L

L

L

-

E

S

T

A

G

N

D

H

K

I

I

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L

S

R

I

I

K

E

G

G

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G

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Y

Y

M

L

L

F

T

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N

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G

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-

A

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Y

F

E

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E

E

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V

L

V

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T

E

E

A

A

Q

K

E

N

L

A

G

G

Y

F

A

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E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

G

S

G

G

Y

T

D

D

A

V

A

A

H

R

K

K

L

V

L

I

I

I

L

L

G

A

S

R

I

E

A

S

-

G

Y

L

G

K

I

L

D

D

A

L

K

A

-

D

-

L

-

P

-

I

-

R

-

S

-

L

-

V

P

P

E

E

E

P

I

L

-

K

-

G

-

C

-

T

-

S

I

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I

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E

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K

I

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P

P

Q

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Y

D

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G

E

D

F

L

A

A

R

K

E

E

-

R

-

L

-

D

-

A

-

E

-

N

F

S

G

G

Y

E

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I

L

K

R

L

Y

L

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G

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K

A

-

V

G

N

E

Q

K

K

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G

-

T

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E

M

L

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H

Y

A

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K

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A

-

K

-

S

-

M

-

I

F

A

A

K

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V

L

D

A

G

G

V

S

M

D

N

N

A

I

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I

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A

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F

V

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G

T

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T

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R

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Y

G

K

E

D

T

H

-

P

-

L

M

I

F

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R

G

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P

P

G

G

A

A

G

G

G

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D

Q

A

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T

A

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G

A

G

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I

I

F

A

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D

D

I

I

V

L

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I

L

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
33% identity, 37% coverage: 67:221/420 of query aligns to 83:239/321 of 3jsaA

query
sites
3jsaA

D

D

V

F

D

D

I

I

V

V

E

D

L
x
A

M
x
T

G
x
P

G

A

I

S

-

A

-

D

-

G

E

K

K

K

P

E

Y

L

R

A

L

F

V

Y

R

K

K

E

A

T

L

F

E

E

A

N

G

G

K

K

P

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

Y

N

H

F

R

W

Y

P

E

E

L

I

Q

M

E

E

I

Y

A

A

-

R

-

S

G

N

E

N

I

R

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R

L

I

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R

Y

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

G

S

A

F

L

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N

Y

G

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L

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A

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N

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R

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K

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K

I

F

K

R

G

G

I

I

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N

S

G

L

T

T

C

I

N

N

Y

Y

M

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L

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M

L

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A

Q

N

E

K

G

R

V

-

A

E

Y

F

E

D

V

V

L

L

K

S

E

E

A

A

Q

T

E

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

T

-

F

-

D

D

V

L

G

T

G

G

Y

L

D
|
D

A

A

H

R

K
|
K

L

S

L

V

I

I

L

L

G

C

S

N

I

H

A

L

Y

Y

G

G

I

S

D

S

A

Y

K

R

P

L

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S

E

D

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I

F

I

Y

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E

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I

active site: D212 (= D194), K216 (= K198)
binding nicotinamide-adenine-dinucleotide: A90 (≠ L74), T91 (≠ M75), P92 (≠ G76), A117 (= A98), N118 (= N99), A144 (= A123)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 11, 12, 13, 14, 42, 43, 308

P31116 Homoserine dehydrogenase; HDH; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 46% coverage: 87:279/420 of query aligns to 104:300/359 of P31116

query
sites
P31116

K

K

A

F

L

V

E

E

A

N

G

G

K

I

P

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

F

G

S

E

N

I

K

P

P

-

T

-

N

-

G

-

F

L

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F

Y

Y

H

E

E

A

A

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T

V

V

A

G

G

A

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I

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G

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G

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Y

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-

S

-

Q

-

A

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V

A

K

Y

F

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S

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D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

G

N

G

G

Y

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

L

T

I

I

L

V

G

G

S

R

I

I

A

S

Y

-

G

G

I

V

D

E

A

V

K

E

P

S

-

P

-

T

-

S

-

F

-

P

-

V

E

Q

E

S

I

L

I

I

I

P

E

K

G

P

I

L

E

E

E

S

I

V

S

K

P

S

T

A

D

D

V

E

E

F

F

L

A

E

R

K

E

L

F

S

G

D

Y

Y

E

D

I

K

K

D

L

L

L

T

G

Q

I

L

A

K

K

K

K

-

G

-

E

E

K

A

G

A

V

T

E

E

M

N

R

K

V

V

H

L

A

-

T

-

M

-

I

-

P

-

A

-

K

-

S

R

M

F

I

I

A

G

K

K

V

V

D

D

G

V

V

A

M

T

N

K

A

S

V

V

S

S

V

V

K117 (= K100) binding NADP(+)
E208 (= E183) binding substrate; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D194) mutation to L: Reduces kcat 150-fold.
K223 (= K198) mutation to V: Loss of activity.

Sites not aligning to the query:

11:18 binding NADP(+)
93 binding NADP(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
28% identity, 46% coverage: 87:279/420 of query aligns to 103:299/358 of 1tveA

query
sites
1tveA

K

K

A

F

L

V

E

E

A

N

G

G

K

I

P

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

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G

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N

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P

P

-

T

-

N

-

G

-

F

L

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F

Y

Y

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E

E

A

A

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V

V

A

G

G

A

G

G

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P

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I

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A

F

L

L

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R

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K

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K

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G

G

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T

C

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K

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F

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Q

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V

V

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E

D

V

V

L

V

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K

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A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

V

L

G

N

G

G

Y

L

D
|
D

A

V

A

A