SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013554960.1 NCBI__GCF_000186245.1:WP_013554960.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8rphD Thiamine pyrophosphate-binding protein
40% identity, 98% coverage: 7:597/603 of query aligns to 5:598/608 of 8rphD

query
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8rphD

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binding (2~{S})-2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-5-[2-[oxidanyl(phosphonooxy)phosphoryl]oxyethyl]-1,3-thiazol-2-yl]-2-oxidanyl-butanoic acid: I26 (≠ S30), E51 (= E55), G405 (= G404), T406 (= T405), G430 (≠ A429), M432 (= M431), C456 (≠ G455), G458 (= G457), G459 (≠ S458), N484 (= N483), G486 (= G485), Y487 (= Y486), L488 (≠ I487), M489 (≠ S488), I490 (= I489)
binding flavin-adenine dinucleotide: R159 (= R166), G219 (= G221), N220 (= N222), G221 (= G223), S245 (≠ T247), W246 (≠ F248), A247 (= A249), A263 (≠ Y265), G264 (= G266), Y266 (≠ V268), G285 (= G287), T286 (≠ A288), R287 (= R289), P291 (≠ R293), Q292 (≠ A294), D310 (= D312), I311 (= I313), D330 (= D332), A331 (= A333), T427 (≠ S426)
binding magnesium ion: D457 (≠ E456), N484 (= N483), G486 (= G485)

8rpjA Janthe from janthinobacterium sp. Hh01
40% identity, 98% coverage: 7:597/603 of query aligns to 5:595/605 of 8rpjA

query
sites
8rpjA

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binding flavin-adenine dinucleotide: R159 (= R166), G219 (= G221), N220 (= N222), G221 (= G223), A225 (≠ S227), S245 (≠ T247), W246 (≠ F248), A247 (= A249), A263 (≠ Y265), G264 (= G266), Y266 (≠ V268), G285 (= G287), T286 (≠ A288), R287 (= R289), P291 (≠ R293), Q292 (≠ A294), D310 (= D312), I311 (= I313), D330 (= D332), A331 (= A333), T427 (≠ S426)
binding magnesium ion: D457 (≠ E456), N484 (= N483), G486 (= G485)
binding thiamine diphosphate: I26 (≠ S30), E51 (= E55), G405 (= G404), T406 (= T405), M432 (= M431), C456 (≠ G455), G458 (= G457), G459 (≠ S458), N484 (= N483), G486 (= G485), Y487 (= Y486), L488 (≠ I487), M489 (≠ S488), I490 (= I489)

8rpiA Janthe from janthinobacterium sp. Hh01, lactyl-thdp
40% identity, 98% coverage: 7:597/603 of query aligns to 5:595/605 of 8rpiA

query
sites
8rpiA

K

K

V

V

S

A

D

E

I

L

V

V

A

A

E

E

F

A

L

L

A

-

E

E

N

N

E

L

Q

G

I

I

A

Q

N

H

D

-

I

A

F

F

M

G

V

I

S

I

G

G

G
x
A

G

G

N

N

M

V

H

H

L

L

I

F

D

E

S

A

I

I

G

A

R

R

N

R

E

G

R

Y

L

T

N

E

Y

I

I

V

C

C

N

V

H

H

H

H

E
|
E

Q

Q

A

A

C

A

A

C

I

M

A

A

A

V

E

Q

G

T

Y

Y

A

Y

R

R

V

T

T

N

N

G

K

R

V

I

G

A

V

A

A

A

M

L

V

L

T

T

T

T

G

G

P

A

G

G

G

S

T

T

N

N

A

G

I

V

T

T

G

G

V

V

Y

V

G

S

A

A

W

W

V

A

D

D

S

S

I

I

P

P

T

C

L

I

T

V

I

I

S

A

G

G

Q

N

V

-

K

-

F

-

E

E

T

N

T

S

V

K

A

F

S

T

Q

F

P

P

E

E

L

N

K

P

L

L

R

R

Q

M

L

W

G

G

D

V

Q

Q

E

G

I

Y

N

D

I

S

I

C

D

Q

V

M

V

V

K

E

P

R

I

V

T

S

K

K

Y

Y

A

Q

V

M

M

R

A

V

T

T

D

K

K

M

Y

E

E

R

I

A

L

V

Y

Y

H

E

L

L

Q

E

K

K

A

G

V

V

Y

H

E

L

A

A

K

L

S

E

G

G

R
|
R

P

P

G

G

P

P

V

T

W

W

V

I

D

E

I

I

P

P

L

M

D

D

I

I

Q

Q

G

S

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N

R

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D

D

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K

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L

L

K

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Y

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-

A

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A

A

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D

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Y

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L

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P

P

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A

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D

A

L

A

K

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V

V

D

D

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V

V

V

L

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L

A

L

L

K

A

Q

K

A

A

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E

R

R

P

P

V

V

I

L

I

W

A

L

G
|
G

N
|
N

G
|
G

I

I

T

R

L

L

S
x
A

G

G

A

G

N

E

E

R

K

L

F

L

L

K

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P

L

L

A

L

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K

K

L

L

G

G

V

S

P

P

V

A

V

L

G

V

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x
S

F
x
W

A
|
A

R

G

Y

I

D

D

I

M

V

L

K

D

T

S

E

S

H

H

P

P

L

L

F

V

F

F

G

G

R

R

Y
x
A

G
|
G

T

V

V
x
Y

G

G

H

Q

R

R

M

A

A

A

N

N

F

F

T

I

V

L

Q

Q

N

N

A

S

D

D

L

Y

V

V

L

L

A

A

I

I

G
|
G

A
x
T

R
|
R

M

L

N

A

I

I

R
x
P

A
x
Q

I

I

S

G

Y

Y

N

D

W

L

E

N

F

E

F

L

A

A

R

R

E

L

A

A

I

R

K

I

I

D

A

V

V

V

D
|
D

I
|
I

D

D

P

G

A

D

E

E

L

A

R

I

K

K

H

H

T

A

L

K

E

R

I

T

D

Q

V

E

P

N

I

I

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V

A

C

D
|
D

A
|
A

K

R

V

V

F

F

I

I

E

E

A

A

M

L

L

L

E

A

R

R

F

L

E

N

N

A

E

A

N

D

M

A

P

P

K

A

Y

I

D

A

K

S

-

K

-

A

-

D

W

W

I

V

E

A

K

K

C

C

L

R

T

A

Y

Y

K

E

K

E

R

Q

Y

F

P

P

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W

I

V

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G

P

A

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H

Q

A

K

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D

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S

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Y

Y

N

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F

F

M

D

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L

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L

L

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N

D

G

M

F

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F

A

K

D

D

D

D

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V

V

F

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V

V

F

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N

M

G
|
G

T
|
T

A

A

C

L

V

L

S

S

S

G

Y

H

Q

Q

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V

L

L

K

R

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K

K

G

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K

G

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K

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V

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N

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x
T

G

G

C

L

A

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M
|
M

G

G

Y

Y

D

G

L

L

P

P

A

A

A

A

I

L

G

G

A

V

A

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F

F

G

A

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N

K

D

S

R

K

G

Q

E

I

V

V

M

C

C

V

L

T

N

G
x
C

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x
D

G
|
G

S
x
G

L

M

Q

M

M

M

N

N

L

L

Q

Q

E

E

L

L

Q

Q

T

T

I

M

I

V

H

H

H

H

R

N

L

L

P

P

V

I

K

K

L

L

F

F

V

I

L

F

N

N

N
|
N

A

D

G
|
G

Y
|
Y

I
x
L

S
x
M

I
|
I

R

K

N

H

T

T

Q

Q

N

K

N

S

F

L

F

F

K

K

G

S

H

D

K

Y

V

V

G

G

S

T

D

D

A

R

S

K

S

S

G

G

V

V

S

S

F

C

P

P

D

D

T

F

L

S

K

R

L

L

A

A

E

A

A

A

Y

F

G

D

F

I

R

P

A

A

F

Y

R

Q

L

I

E

R

T

G

Q

W

L

D

D

E

L

C

V

D

R

A

Q

T

L

L

E

A

N

K

I

V

L

Q

R

A

I

H

E

T

G

G

P

P

V

V

I

I

C

C

E

E

V

V

M

F

L

M

S

H

P

P

T

Q

E

Q

K

L

M

F

E

S

P

P

K

K

L

L

S

G

S

V

E

V

V

S

K

R

P

-

D

-

G

-

T

T

M

L

I

V

S

S

K

P

P

P

L

L

E

E

D

D

M

L

F

S

P

P

F

L

L

I

D

P

R

R

K

D

E

V

F

L

F

E

E

Q

N

A

M

M

I

I

binding flavin-adenine dinucleotide: R159 (= R166), G219 (= G221), N220 (= N222), G221 (= G223), A225 (≠ S227), S245 (≠ T247), W246 (≠ F248), A247 (= A249), A263 (≠ Y265), G264 (= G266), Y266 (≠ V268), G285 (= G287), T286 (≠ A288), R287 (= R289), P291 (≠ R293), Q292 (≠ A294), D310 (= D312), I311 (= I313), D330 (= D332), A331 (= A333), T427 (≠ S426)
binding magnesium ion: D457 (≠ E456), N484 (= N483), G486 (= G485)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: A28 (≠ G32), E51 (= E55), G405 (= G404), T406 (= T405), M432 (= M431), C456 (≠ G455), G458 (= G457), G459 (≠ S458), N484 (= N483), G486 (= G485), Y487 (= Y486), L488 (≠ I487), M489 (≠ S488), I490 (= I489)

5ahkA Crystal structure of acetohydroxy acid synthase pf5 from pseudomonas protegens
32% identity, 93% coverage: 7:565/603 of query aligns to 2:543/555 of 5ahkA

query
sites
5ahkA

K

K

V

A

S

S

D

D

I

A

V

V

A

A

E

K

F

I

L

L

A

A

E

D

N

N

E

N

Q

V

I

L

A

Y

N

G

D

-

I

-

F

F

M

E

V
x
L

S
x
I

G
|
G

G
|
G

G
x
M

N
x
I

M

T

H

H

L

L

I

V

D

D

S

S

I

I

G

N

R

L

N

L

E

G

R

K

L

T

N

K

Y

L

I

V

C

S

N

M

H

H

H

H

E
|
E

Q

Q

A

G

C

A

A

A

I

F

A

A

A

A

E

S

G

A

Y

V

A

S

R

R

V

V

T

T

N

H

K

H

-

K

-

T

V

L

G

G

V

L

A

A

M

L

V

A

T
|
T

T

S

G

G

P
|
P

G

G

G

A

T

T

N

N

A

L

I

I

T

T

G

G

V

I

Y

A

G

D

A

C

W

W

V

L

D

D

S

S

I

H

P

P

T

C

L

I

T

F

I

L

S

T

G

G

Q

Q

V

V

K

-

F

-

E

-

T

N

T

T

V

H

A

E

S

L

Q

K

P

G

E
x
K

L
x
R

K

D

L

I

R

R

Q

Q

L

Q

G

G

D
x
F

Q
|
Q

E

E

I

L

N

D

I

S

I

V

D

A

V

L

V

V

K

T

P

S

I

I

T

T

K

K

Y

Y

A

A

V

Y

M

Q

A

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T

K

D

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K

A

Y

D

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I

L

L

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Y

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L

L

Q

R

K

K

A

A

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I

Y

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A

A

K

K

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G

G

R
|
R

P

P

G

G

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P

V

V

W

L

V

L

D

D

I

I

P

P

L
x
M

D

D

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Q

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V

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D

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K

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L

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D

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V

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D

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V

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N

L

K

L

L

K

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Q

N

A

A

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K

R

K

P

P

V

L

I

L

I

L

A

I

G
|
G

N
x
G

G
|
G

I

A

T

V

L

N

S

S

G

S

A

G

N

F

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Q

K

K

F

W

L

L

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M

Y
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G

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G

H

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A

A

A

N

N

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Q

N

N

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C

D

D

L

L

V

L

L

L

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|
G

A
x
S

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|
R

M

M

N
x
D

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|
R

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|
G

N
|
N

G
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N

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|
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N

N
|
N

A

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x
S

Y
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L

I
x
G

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x
M

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x
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Y

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F

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G

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G

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L

L

active site: L22 (≠ V29), G24 (= G31), G25 (= G32), M26 (≠ G33), I27 (≠ N34), E48 (= E55), T73 (= T78), K110 (≠ E118), R111 (≠ L119), F118 (≠ D126), Q119 (= Q127), M168 (≠ L176), A253 (≠ T267), Q280 (≠ A294), V384 (≠ F401), G410 (≠ A429), M412 (= M431), D436 (≠ E456), N463 (= N483), S465 (≠ G485), L466 (≠ Y486), M468 (≠ S488), V469 (≠ I489), F472 (≠ T492), D535 (≠ P557)
binding flavin-adenine dinucleotide: R158 (= R166), G208 (= G221), G209 (≠ N222), G210 (= G223), S234 (≠ T247), L235 (≠ F248), M250 (≠ R264), L251 (≠ Y265), Y254 (≠ V268), G273 (= G287), S274 (≠ A288), R275 (= R289), D277 (≠ N291), R279 (= R293), Q280 (≠ A294), D298 (= D312), L299 (≠ I313), Q303 (≠ E317), E317 (≠ F336), L318 (≠ I337), M389 (≠ A406), G407 (≠ S426)
binding magnesium ion: D436 (≠ E456), N463 (= N483), S465 (≠ G485)
binding thiamine diphosphate: I23 (≠ S30), E48 (= E55), P76 (= P81), V384 (≠ F401), G385 (= G402), N386 (= N403), N387 (≠ G404), G410 (≠ A429), M412 (= M431), D436 (≠ E456), G437 (= G457), G438 (≠ S458), N463 (= N483), S465 (≠ G485), L466 (≠ Y486), G467 (≠ I487), M468 (≠ S488), V469 (≠ I489)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
31% identity, 93% coverage: 6:567/603 of query aligns to 91:647/664 of P09114

query
sites
P09114

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P191 (= P117) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (≠ T492) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
31% identity, 93% coverage: 6:567/603 of query aligns to 94:650/667 of P09342

query
sites
P09342

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C161 (≠ A75) modified: Disulfide link with 307
P194 (= P117) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ I224) modified: Disulfide link with 161

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/585 of 5k2oA

query
sites
5k2oA

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S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ T267), R292 (= R293), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), G286 (= G287), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), M428 (= M431), D453 (≠ E456), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/583 of 5k3sA

query
sites
5k3sA

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

E

-

N

E

E

R

Q

Q

I

G

A

V

N

E

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T

I

V

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F

M

A

V
x
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S

P

G
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G

G
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G

G
x
A

N
x
S

M

M

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E

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I

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A

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Q

Q

A

G

C

G

A

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A

A

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E

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Y

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A

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T

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