SitesBLAST

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
31% identity, 93% coverage: 6:567/603 of query aligns to 91:647/664 of P09114

query
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P09114

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P191 (= P117) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (≠ T492) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
31% identity, 93% coverage: 6:567/603 of query aligns to 94:650/667 of P09342

query
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P09342

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C161 (≠ A75) modified: Disulfide link with 307
P194 (= P117) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ I224) modified: Disulfide link with 161

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/585 of 5k2oA

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5k2oA

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P

Y

R

H

I

L

I

Q

E

K

E

A

A

V

F

Y

F

E

L

A

A

K

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

V

V

D

D

I

V

P

P

L
x
K

D

D

I

I

Q

Q

G

Q

-

Q

-

L

-

A

-

I

A

P

N

N

V

W

D

E

E

Q

K

A

-

M

R

R

L

L

K

P

Q

G

F

Y

V

M

P

S

-

R

-

M

P

P

A

K

P

P

E

E

Y

-

D

D

L

S

K

H

V

L

D

E

E

Q

V

I

V

V

S

R

L

L

L

I

K

S

Q

E

A

S

E

K

R

K

P

P

V

V

I

L

I

Y

A

V

G
|
G

N
x
G

G
|
G

I

C

T

L

L

N

S

S

G

-

A

-

N

S

E

D

K

E

F

L

L

G

Q

R

L

F

A

V

E

E

K

L

L

T

G

G

V

I

P

P

V

V

V

A

G

S

T
|
T

F
x
L

A
x
M

R

G

Y

L

D

G

I

S

V

Y

K

P

T

C

E

D

H

D

P

E

L

L

F

S

F

L

G

H

R

M

Y
x
L

G

G

T
x
M

V

H

G

G

H

T

R

V

M

Y

A

A

N

N

F

Y

T

A

V

V

Q

E

N

H

A

S

D

D

L

L

V

L

L

L

A

A

I

F

G
|
G

A

V

R
|
R

M

F

N
x
D

I

D

R
|
R

A

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

F

A

F

F

A

A

R

S

E

R

A

A

I

K

K

I

I

V

A

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

L

I

R

G

K

K

H

N

T

K

L

T

E

P

I

-

D

H

V

V

P

S

I

V

E

C

A

G

D
|
D

A
x
V

K

K

V

L

F

A

I

L

E

Q

A

G

M

M

L

N

E

K

R

V

F

L

E

E

N

N

-

R

-

A

-

E

E

E

N

L

M

K

P

L

K

D

Y

F

D

G

K

V

W

W

I

R

E

N

K

E

C

L

L

N

T

V

Y

Q

K

K

K

Q

R

K

Y

F

P

P

T

L

I

S

E

F

P

K

T

T

R

F

Q

G

K

E

I

A

V

I

D

P

T

P

V

Q

D

Y

S

A

Y

I

N

K

F

V

F

L

D

D

L

E

L

L

S

T

D

D

M

G

T

K

A

A

D

-

T

-

V

I

F

I

V

S

F

T

G

G

N
x
V

G
|
G

T
x
Q

A
x
H

C
x
Q

V
x
M

S

W

S

A

Y

A

Q

Q

S

F

L

Y

K

N

V

Y

K

K

G

K

K

P

Q

R

K

Q

V

W

V

L

V

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

D

G

L

L

P

P

A

A

I

I

G

G

A

A

A

S

F

V

G

A

S

N

K

P

S

D

K

A

Q

I

I

V

V

V

C

D

V

I

T

D

G

G

E
x
D

G
|
G

S
|
S

L

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

H

E

R

N

L

L

P

P

V

V

K

K

L

V

F

L

V

L

L

L

N

N

N
|
N

A

Q

G
x
H

Y
x
L

I
x
G

S
x
M

I
x
V

R

M

N

Q

T
x
W

Q

E

N

D

N

R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

S

D

D

P

A

A

S

Q

S

E

G

D

V

E

S

I

F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

F

I

R

P

A

A

F

A

R

R

L

V

E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

Q

A

L

I

E

Q

N

T

I

M

L

L

R

D

I

T

E

P

G

G

P

P

V

Y

I

L

C

L

E

D

V

V

M

I

L

C

S

P

P
x
H

T

Q

E

E

K

H

M

V

E

L

P

P

K

M

L

I

S

P

S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ T267), R292 (= R293), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), G286 (= G287), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), M428 (= M431), D453 (≠ E456), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/583 of 5k3sA

query
sites
5k3sA

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

E

-

N

E

E

R

Q

Q

I

G

A

V

N

E

D

T

I

V

F

F

M

A

V
x
Y

S

P

G
|
G

G
x
A

N
x
S

M

M

H

E

L

I

I

H

D

Q

S

A

I

L

G

T

R

R

N

S

E

S

R

S

L

I

N

R

Y

N

I

V

C

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

G

G

Y

Y

A

A

R

R

V

S

T

S

N

G

K

K

V

P

G

G

V

I

A

C

M

I

V

A

T
|
T

T

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

Y

A

G

D

A

A

W

L

V

L

D

D

S

S

I

V

P

P

T

L

L

V

T

A

I

I

S

T

G

G

Q

Q

V

V

K

-

F

-

E

-

T

-

V

-

A

-

S

-

Q

-

P

P

E

R

L

R

K

M

L

I

R

G

Q

T

L

D

G

A

D
x
F

Q
|
Q

E
|
E

I

T

N

P

I

I

I

V

D

E

V

V

V

T

K

R

P

S

I

I

T

T

K

K

Y

H

A

N

V

Y

M

L

A

V

T

M

D

D

K

V

Y

E

E

D

I

I

L

P

Y

R

H

I

L

I

Q

E

K

E

A

A

V

F

Y

F

E

L

A

A

K

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

V

V

D

D

I

V

P

P

L
x
K

D

D

I

I

Q

Q

G

Q

-

Q

-

L

-

A

-

I

A

P

N

N

V

W

D

E

E

Q

K

A

-

M

R

R

L

L

K

P

Q

G

F

Y

V

M

P

S

-

R

-

M

P

P

A

K

P

P

E

E

Y

-

D

D

L

S

K

H

V

L

D

E

E

Q

V

I

V

V

S

R

L

L

L

I

K

S

Q

E

A

S

E

K

R

K

P

P

V

V

I

L

I

Y

A

V

G
|
G

N
x
G

G
|
G

I

C

T

L

L

N

S

S

G

-

A

-

N

S

E

D

K

E

F

L

L

G

Q

R

L

F

A

V

E

E

K

L

L

T

G

G

V

I

P

P

V

V

V

A

G

S

T
|
T

F
x
L

A
x
M

R

G

Y

L

D

G

I

S

V

Y

K

P

T

C

E

D

H

D

P

E

L

L

F

S

F

L

G

H

R

M

Y
x
L

G

G

T
x
M

V

H

G

G

H

T

R

V

M

Y

A

A

N

N

F

Y

T

A

V

V

Q

E

N

H

A

S

D

D

L

L

V

L

L

L

A

A

I

F

G
|
G

A

V

R
|
R

M

F

N
x
D

I

D

R
|
R

A

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

F

A

F

F

A

A

R

S

E

R

A

A

I

K

K

I

I

V

A

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

L

I

R

G

K

K

H

N

T

K

L

T

E

P

I

-

D

H

V

V

P

S

I

V

E

C

A

G

D
|
D

A
x
V

K

K

V

L

F

A

I

L

E

Q

A

G

M

M

L

N

E

K

R

V

F

L

E

E

N

N

-

R

-

A

-

E

E

E

N

L

M

K

P

L

K

D

Y

F

D

G

K

V

W

W

I

R

E

N

K

E

C

L

L

N

T

V

Y

Q

K

K

K

Q

R

K

Y

F

P

P

T

L

I

S

E

F

P

K

T

T

R

F

Q

G

K

E

I

A

V

I

D

P

T

P

V

Q

D

Y

S

A

Y

I

N

K

F

V

F

L

D

D

L

E

L

L

S

T

D

D

M

G

T

K

A

A

D

-

T

-

V

I

F

I

V

S

F

T

G

G

N
x
V

G
|
G

T
x
Q

A
x
H

C

Q

V
x
M

S

W

S

A

Y

A

Q

Q

S

F

L

Y

K

N

V

Y

K

K

G

K

K

P

Q

R

K

Q

V

W

V

L

V

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

D

G

L

L

P

P

A

A

I

I

G

G

A

A

A

S

F

V

G

A

S

N

K

P

S

D

K

A

Q

I

I

V

V

V

C

D

V

I

T

D

G

G

E
x
D

G
|
G

S
|
S

L

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

H

E

R

N

L

L

P

P

V

V

K

K

L

V

F

L

V

L

L

L

N

N

N
|
N

A

Q

G
x
H

Y
x
L

I
x
G

S
x
M

I
x
V

R

M

N

Q

T
x
W

Q

E

N

D

N

R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

S

D

D

P

A

A

S

Q

S

E

G

D

V

E

S

I

F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

F

I

R

P

A

A

F

A

R

R

L

V

E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

Q

A

L

I

E

Q

N

T

I

M

L

L

R

D

I

T

E

P

G

G

P

P

V

Y

I

L

C

L

E

D

V

V

M

I

L

C

S

P

P
x
H

T

Q

E

E

K

H

M

V

E

L

P

P

K

M

L

I

S

P

S

S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R293), M485 (≠ S488), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), M266 (≠ T267), G286 (= G287), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), D453 (≠ E456), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 569

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 8et4A

query
sites
8et4A

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

E

-

N

E

E

R

Q

Q

I

G

A

V

N

E

D

T

I

V

F

F

M

A

V

Y

S

P

G

G

G

A

N

S

M

M

H

E

L

I

I

H

D

Q

S

A

I

L

G

T

R

R

N

S

E

S

R

S

L

I

N

R

Y

N

I

V

C

L

N

P

H

R

H

H

E

E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

G

G

Y

Y

A

A

R

R

V

S

T

S

N

G

K

K

V

P

G

G

V

I

A

C

M

I

V

A

T

T

T

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

Y

A

G

D

A

A

W

L

V

L

D

D

S

S

I

V

P

P

T

L

L

V

T

A

I

I

S

T

G

G

Q

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-

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F

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N

L

L

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L

L

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x
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x
L

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x
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x
M

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x
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M

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D

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R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

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D

D

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A

A

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P

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N

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M

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L

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L

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A

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A

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C

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G

F

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A

A

F

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x
K

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D

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S
|
S

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), G452 (= G455), D453 (≠ E456), G454 (= G457), S455 (= S458), M458 (= M461), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: F370 (≠ E370), D453 (≠ E456), M458 (= M461), Q461 (= Q464), N480 (= N483), H482 (≠ G485), K533 (≠ Q532)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ T267), R292 (= R293), M485 (≠ S488), W489 (≠ T492), S568 (= S567)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 5wj1A

query
sites
5wj1A

K

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K

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N

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E

E

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I

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I

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H

E

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N

L

L

P

P

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K

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F

L

V

L

L

L

N

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|
N

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x
H

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x
L

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x
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x
M

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x
V

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M

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x
W

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E

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D

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R

F

F

F

Y

K

K

G

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H

N

K

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-

A

-

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-

F

V

L

G

G

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D

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A

A

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G

D

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S

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F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

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A

A

A

Y

C

G

G

F

I

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A

A

F

A

R

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E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

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A

L

I

E

Q

N

T

I

M

L

L

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D

I

T

E

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G

G

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Y

I

L

C

L

E

D

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M

I

L

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P
x
H

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E

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K

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M

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K

M

L

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S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), M263 (≠ R264), L264 (≠ Y265), G286 (= G287), R288 (= R289), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ T267), D291 (≠ I292), R292 (= R293), M485 (≠ S488), W489 (≠ T492), S568 (= S567)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), M428 (= M431), D453 (≠ E456), G454 (= G457), S455 (= S458), M458 (= M461), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 5k6tA

query
sites
5k6tA

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

E

-

N

E

E

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Q

Q

I

G

A

V

N

E

D

T

I

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F

M

A

V
x
Y

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G
|
G

G
x
A

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x
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M

M

H

E

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I

H

D

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S

A

I

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R

N

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E

S

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S

L

I

N

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Y

N

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H

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H

H

E
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E

Q

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A

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C

G

A

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A

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G

Y

Y

A

A

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K

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T

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G

G

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G

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-

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F

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E

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R

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x
K

D

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L

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G

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x
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R

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V

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G

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x
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M

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M

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G

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L

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P

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A

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I

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G

A

A

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S

L

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M

M

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N

L

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E

E

L

L

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A

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I

I

I

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H

E

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N

L

L

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P

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V

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K

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F

L

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L

L

L

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x
H

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x
L

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x
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x
M

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x
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x
W

Q

E

N

D

N

R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

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D

D

P

A

A

S

Q

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E

G

D

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E

S

I

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F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

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G

F

I

R

P

A

A

F

A

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R

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E

T

T

K

Q

K

L

A

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D

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A

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E

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H

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S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ V268), R292 (= R293), M485 (≠ S488), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), G286 (= G287), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), G452 (= G455), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 5k6rA

query
sites
5k6rA

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

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-

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E

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A

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E

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F

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x
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G

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x
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M

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I

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A

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Y

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E

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Q

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C

G

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I

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A

A

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E

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Y

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K

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G

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P

G

G

G

A

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T

N

N

A

L

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G

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A

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A

W

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L

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-

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R

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K

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G

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G

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I

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V

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M

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K

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M

G

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A

A

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I

V

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V

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G

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x
Q

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x
H

C

Q

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x
M

S

W

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Y

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Y

K

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P

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Q

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W

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S

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x
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x
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M

G

G

Y

F

D

G

L

L

P

P

A

A

I

I

G

G

A

A

A

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F

V

G

A

S

N

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D

K

A

Q

I

I

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V

V

C

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V

I

T

D

G

G

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x
D

G
|
G

S
|
S

L

F

Q

I

M
|
M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

H

E

R

N

L

L

P

P

V

V

K

K

L

V

F

L

V

L

L

L

N

N

N
|
N

A

Q

G
x
H

Y
x
L

I
x
G

S
x
M

I
x
V

R

M

N

Q

T
x
W

Q

E

N

D

N

R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

S

D

D

P

A

A

S

Q

S

E

G

D

V

E

S

I

F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

F

I

R

P

A

A

F

A

R

R

L

V

E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

Q

A

L

I

E

Q

N

T

I

M

L

L

R

D

I

T

E

P

G

G

P

P

V

Y

I

L

C

L

E

D

V

V

M

I

L

C

S

P

P
x
H

T

Q

E

E

K

H

M

V

E

L

P

P

K

M

L

I

S

P

S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R293), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), M266 (≠ T267), G286 (= G287), R288 (= R289), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), G328 (≠ A331), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), D453 (≠ E456), G454 (= G457), S455 (= S458), M458 (= M461), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 1z8nA

query
sites
1z8nA

K

R

K

K

V

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I

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S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K140), R161 (= R166), Y191 (≠ F191), R194 (vs. gap), D291 (≠ I292), R292 (= R293), D312 (= D314), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), G265 (= G266), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483)
binding thiamine diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ T405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), G452 (= G455), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ I487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: 569

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 1yi1A

query
sites
1yi1A

K

R

K

K

V

G

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A

D

D

I

I

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E

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A

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L

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I

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V

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N

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L

P

P

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F

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V

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L

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N

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x
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x
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I

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x
M

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x
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x
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E

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D

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F

F

F

Y

K

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H

N

K

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-

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-

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-

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D

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A

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A

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V

E

T

T

K

Q

K

L

A

D

D

L

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A

L

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E

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N

T

I

M

L

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Y

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L

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L

E

D

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V

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I

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S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ I292), R292 (= R293), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), M263 (≠ R264), L264 (≠ Y265), G265 (= G266), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 1yi0A

query
sites
1yi0A

K

R

K

K

V

G

S

A

D

D

I

I

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L

A

V

E

E

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A

L

L

A

-

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x
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|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ I292), R292 (= R293), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), G265 (= G266), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), G328 (≠ A331), D329 (= D332), V330 (≠ A333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 1yhzA

query
sites
1yhzA

K

R

K

K

V

G

S

A

D

D

I

I

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V

E

E

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A

L

L

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-

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-

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E

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A

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I

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A

A

E

E

G

G

Y

Y

A

A

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A

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A

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|
T

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G

G

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P

G

G

G

A

T

T

N

N

A

L

I

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G

G

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Y

A

G

D

A

A

W

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G

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-

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I

S

P

S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ I292), R292 (= R293), M485 (≠ S488), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 1yhyA

query
sites
1yhyA

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

E

-

N

E

E

R

Q

Q

I

G

A

V

N

E

D

T

I

V

F

F

M

A

V
x
Y

S

P

G
|
G

G
x
A

N
x
S

M

M

H

E

L

I

I

H

D

Q

S

A

I

L

G

T

R

R

N

S

E

S

R

S

L

I

N

R

Y

N

I

V

C

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

G

G

Y

Y

A

A

R

R

V

S

T

S

N

G

K

K

V

P

G

G

V

I

A

C

M

I

V

A

T
|
T

T

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

Y

A

G

D

A

A

W

L

V

L

D

D

S

S

I

V

P

P

T

L

L

V

T

A

I

I

S

T

G

G

Q

Q

V

V

K

-

F

-

E

-

T

-

V

-

A

-

S

-

Q

-

P

P

E

R

L

R

K

M

L

I

R

G

Q

T

L

D

G

A

D
x
F

Q
|
Q

E
|
E

I

T

N

P

I

I

I

V

D

E

V

V

V

T

K

R

P

S

I

I

T

T

K

K

Y

H

A

N

V

Y

M

L

A

V

T

M

D

D

K

V

Y

E

E

D

I

I

L

P

Y

R

H

I

L

I

Q

E

K

E

A

A

V

F

Y

F

E

L

A

A

K

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

V

V

D

D

I

V

P

P

L
x
K

D

D

I

I

Q

Q

G

Q

-

Q

-

L

-

A

-

I

A

P

N

N

V

W

D

E

E

Q

K

A

-

M

R

R

L

L

K

P

Q

G

F

Y

V

M

P

S

-

R

-

M

P

P

A

K

P

P

E

E

Y

-

D

D

L

S

K

H

V

L

D

E

E

Q

V

I

V

V

S

R

L

L

L

I

K

S

Q

E

A

S

E

K

R

K

P

P

V

V

I

L

I

Y

A

V

G
|
G

N
x
G

G
|
G

I

C

T

L

L

N

S

S

G

-

A

-

N

S

E

D

K

E

F

L

L

G

Q

R

L

F

A

V

E

E

K

L

L

T

G

G

V

I

P

P

V

V

V

A

G

S

T
|
T

F
x
L

A
x
M

R

G

Y

L

D

G

I

S

V

Y

K

P

T

C

E

D

H

D

P

E

L

L

F

S

F

L

G

H

R

M

Y
x
L

G
|
G

T
x
M

V
x
H

G

G

H

T

R

V

M

Y

A

A

N

N

F

Y

T

A

V

V

Q

E

N

H

A

S

D

D

L

L

V

L

L

L

A

A

I

F

G
|
G

A
x
V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

A

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

F

A

F

F

A

A

R

S

E

R

A

A

I

K

K

I

I

V

A

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

L

I

R

G

K

K

H

N

T

K

L

T

E

P

I

-

D

H

V

V

P

S

I

V

E

C

A

G

D
|
D

A
x
V

K

K

V

L

F

A

I

L

E

Q

A

G

M

M

L

N

E

K

R

V

F

L

E

E

N

N

-

R

-

A

-

E

E

E

N

L

M

K

P

L

K

D

Y

F

D

G

K

V

W

W

I

R

E

N

K

E

C

L

L

N

T

V

Y

Q

K

K

K

Q

R

K

Y

F

P

P

T

L

I

S

E

F

P

K

T

T

R

F

Q

G

K

E

I

A

V

I

D

P

T

P

V

Q

D

Y

S

A

Y

I

N

K

F

V

F

L

D

D

L

E

L

L

S

T

D

D

M

G

T

K

A

A

D

-

T

-

V

I

F

I

V

S

F

T

G

G

N
x
V

G

G

T

Q

A

H

C
x
Q

V
x
M

S

W

S

A

Y

A

Q

Q

S

F

L

Y

K

N

V

Y

K

K

G

K

K

P

Q

R

K

Q

V

W

V

L

V

S

N

S

S
x
G

G
|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

D

G

L

L

P

P

A

A

I

I

G

G

A

A

A

S

F

V

G

A

S

N

K

P

S

D

K

A

Q

I

I

V

V

V

C

D

V

I

T

D

G

G

E
x
D

G

G

S

S

L

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

H

E

R

N

L

L

P

P

V

V

K

K

L

V

F

L

V

L

L

L

N

N

N
|
N

A

Q

G
x
H

Y
x
L

I

G

S
x
M

I
x
V

R

M

N

Q

T
x
W

Q

E

N

D

N

R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

S

D

D

P

A

A

S

Q

S

E

G

D

V

E

S

I

F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

F

I

R

P

A

A

F

A

R

R

L

V

E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

Q

A

L

I

E

Q

N

T

I

M

L

L

R

D

I

T

E

P

G

G

P

P

V

Y

I

L

C

L

E

D

V

V

M

I

L

C

S

P

P
x
H

T

Q

E

E

K

H

M

V

E

L

P

P

K

M

L

I

S

P

S
|
S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ I292), R292 (= R293), V486 (≠ I489), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G222 (= G221), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), G265 (= G266), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 12:568/582 of 1ybhA

query
sites
1ybhA

K

R

K

K

V

G

S

A

D

D

I

I

V

L

A

V

E

E

F

A

L

L

A

-

E

-

N

E

E

R

Q

Q

I

G

A

V

N

E

D

T

I

V

F

F

M

A

V
x
Y

S

P

G
|
G

G
x
A

N
x
S

M

M

H

E

L

I

I

H

D

Q

S

A

I

L

G

T

R

R

N

S

E

S

R

S

L

I

N

R

Y

N

I

V

C

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

G

G

Y

Y

A

A

R

R

V

S

T

S

N

G

K

K

V

P

G

G

V

I

A

C

M

I

V

A

T
|
T

T

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

Y

A

G

D

A

A

W

L

V

L

D

D

S

S

I

V

P

P

T

L

L

V

T

A

I

I

S

T

G

G

Q

Q

V

V

K

-

F

-

E

-

T

-

V

-

A

-

S

-

Q

-

P

P

E

R

L

R

K

M

L

I

R

G

Q

T

L

D

G

A

D
x
F

Q
|
Q

E
|
E

I

T

N

P

I

I

I

V

D

E

V

V

V

T

K

R

P

S

I

I

T

T

K

K

Y

H

A

N

V

Y

M

L

A

V

T

M

D

D

K

V

Y

E

E

D

I

I

L

P

Y

R

H

I

L

I

Q

E

K

E

A

A

V

F

Y

F

E

L

A

A

K

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

V

V

D

D

I

V

P

P

L
x
K

D

D

I

I

Q

Q

G

Q

-

Q

-

L

-

A

-

I

A

P

N

N

V

W

D

E

E

Q

K

A

-

M

R

R

L

L

K

P

Q

G

F

Y

V

M

P

S

-

R

-

M

P

P

A

K

P

P

E

E

Y

-

D

D

L

S

K

H

V

L

D

E

E

Q

V

I

V

V

S

R

L

L

L

I

K

S

Q

E

A

S

E

K

R

K

P

P

V

V

I

L

I

Y

A

V

G

G

N
x
G

G
|
G

I

C

T

L

L

N

S

S

G

-

A

-

N

S

E

D

K

E

F

L

L

G

Q

R

L

F

A

V

E

E

K

L

L

T

G

G

V

I

P

P

V

V

V

A

G

S

T
|
T

F
x
L

A
x
M

R

G

Y

L

D

G

I

S

V

Y

K

P

T

C

E

D

H

D

P

E

L

L

F

S

F

L

G

H

R

M

Y
x
L

G

G

T
x
M

V
x
H

G

G

H

T

R

V

M

Y

A

A

N

N

F

Y

T

A

V

V

Q

E

N

H

A

S

D

D

L

L

V

L

L

L

A

A

I

F

G
|
G

A
x
V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

A

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

F

A

F

F

A

A

R

S

E

R

A

A

I

K

K

I

I

V

A

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

L

I

R

G

K

K

H

N

T

K

L

T

E

P

I

-

D

H

V

V

P

S

I

V

E

C

A

G

D
|
D

A
x
V

K

K

V

L

F

A

I

L

E

Q

A

G

M

M

L

N

E

K

R

V

F

L

E

E

N

N

-

R

-

A

-

E

E

E

N

L

M

K

P

L

K

D

Y

F

D

G

K

V

W

W

I

R

E

N

K

E

C

L

L

N

T

V

Y

Q

K

K

K

Q

R

K

Y

F

P

P

T

L

I

S

E

F

P

K

T

T

R

F

Q

G

K

E

I

A

V

I

D

P

T

P

V

Q

D

Y

S

A

Y

I

N

K

F

V

F

L

D

D

L

E

L

L

S

T

D

D

M

G

T

K

A

A

D

-

T

-

V

I

F

I

V

S

F

T

G

G

N
x
V

G

G

T

Q

A

H

C
x
Q

V
x
M

S

W

S

A

Y

A

Q

Q

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F

L

Y

K

N

V

Y

K

K

G

K

K

P

Q

R

K

Q

V

W

V

L

V

S

N

S

S
x
G

G
|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

D

G

L

L

P

P

A

A

I

I

G

G

A

A

A

S

F

V

G

A

S

N

K

P

S

D

K

A

Q

I

I

V

V

V

C

D

V

I

T

D

G

G

E
x
D

G

G

S

S

L

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

H

E

R

N

L

L

P

P

V

V

K

K

L

V

F

L

V

L

L

L

N

N

N
|
N

A

Q

G
x
H

Y
x
L

I

G

S
x
M

I
x
V

R

M

N

Q

T
x
W

Q

E

N

D

N

R

F

F

F

Y

K

K

G

A

H

N

K

R

-

A

-

H

-

T

-

F

V

L

G

G

S

D

D

P

A

A

S

Q

S

E

G

D

V

E

S

I

F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

F

I

R

P

A

A

F

A

R

R

L

V

E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

Q

A

L

I

E

Q

N

T

I

M

L

L

R

D

I

T

E

P

G

G

P

P

V

Y

I

L

C

L

E

D

V

V

M

I

L

C

S

P

P
x
H

T

Q

E

E

K

H

M

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S

active site: Y33 (≠ V29), G35 (= G31), G36 (= G32), A37 (≠ G33), S38 (≠ N34), E59 (= E55), T82 (= T78), F121 (≠ D126), Q122 (= Q127), E123 (= E128), K171 (≠ L176), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (≠ E456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492), H558 (≠ P557)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (≠ T267), D291 (≠ I292), R292 (= R293), M485 (≠ S488), W489 (≠ T492), S568 (= S567)
binding flavin-adenine dinucleotide: R161 (= R166), G223 (≠ N222), G224 (= G223), T246 (= T247), L247 (≠ F248), M248 (≠ A249), L264 (≠ Y265), M266 (≠ T267), H267 (≠ V268), G286 (= G287), V287 (≠ A288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ A333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (≠ E456), N480 (= N483), H482 (≠ G485)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
30% identity, 93% coverage: 6:567/603 of query aligns to 97:653/670 of P17597

query
sites
P17597

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S

A122 (≠ G33) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M35) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E55) binding thiamine diphosphate
S186 (= S97) binding FAD
P197 (= P117) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ L119) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q127) binding thiamine diphosphate
K220 (= K140) binding (R)-imazaquin
R246 (= R166) binding (R)-imazaquin; binding FAD
K256 (≠ L176) binding chlorimuron-ethyl
G308 (≠ N222) binding FAD
TL 331:332 (≠ TF 247:248) binding FAD
C340 (≠ T256) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ YGTV 265:268) binding FAD
GVRFD 371:375 (≠ GARMN 287:291) binding FAD
DR 376:377 (≠ IR 292:293) binding chlorimuron-ethyl
DI 395:396 (= DI 312:313) binding FAD
DV 414:415 (≠ DA 332:333) binding FAD
QH 487:488 (≠ TA 405:406) binding thiamine diphosphate
GG 508:509 (≠ SG 426:427) binding FAD
GAM 511:513 (≠ ASM 429:431) binding thiamine diphosphate
D538 (≠ E456) binding Mg(2+)
DGS 538:540 (≠ EGS 456:458) binding thiamine diphosphate
N565 (= N483) binding Mg(2+)
NQHLGM 565:570 (≠ NAGYIS 483:488) binding thiamine diphosphate
H567 (≠ G485) binding Mg(2+)
W574 (≠ T492) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (= S567) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
30% identity, 93% coverage: 6:567/603 of query aligns to 11:567/582 of 3ea4A

query
sites
3ea4A

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T

-

F

V

L

G

G

S

D

D

P

A

A

S

Q

S

E

G

D

V

E

S

I

F

F

P

P

D

N

T

M

L

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

F

I

R

P

A

A

F

A

R

R

L

V

E

T

T

K

Q

K

L

A

D

D

L

L

V

R

R

E

Q

A

L

I

E

Q

N

T

I

M

L

L

R

D

I

T

E

P

G

G

P

P

V

Y

I

L

C

L

E

D

V

V

M

I

L

C

S

P

P
x
H

T

Q

E

E

K

H

M

V

E

L

P

P

K

M

L

I

S

P

S
|
S

active site: Y32 (≠ V29), G34 (= G31), G35 (= G32), A36 (≠ G33), S37 (≠ N34), E58 (= E55), T81 (= T78), F120 (≠ D126), Q121 (= Q127), E122 (= E128), K170 (≠ L176), M265 (≠ T267), V292 (≠ I295), V399 (≠ N403), G425 (≠ A429), M427 (= M431), D452 (≠ E456), N479 (= N483), H481 (≠ G485), L482 (≠ Y486), M484 (≠ S488), V485 (≠ I489), W488 (≠ T492), H557 (≠ P557)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ I292), R291 (= R293), W488 (≠ T492), S567 (= S567)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R166), G221 (= G221), G222 (≠ N222), G223 (= G223), T245 (= T247), L246 (≠ F248), M247 (≠ A249), L263 (≠ Y265), G264 (= G266), M265 (≠ T267), H266 (≠ V268), G285 (= G287), R287 (= R289), D289 (≠ N291), R291 (= R293), D309 (= D312), I310 (= I313), G327 (≠ A331), D328 (= D332), V329 (≠ A333), M404 (≠ V408), G422 (≠ S426)
binding magnesium ion: D452 (≠ E456), N479 (= N483), H481 (≠ G485)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N403), G400 (= G404), Q401 (≠ T405), H402 (≠ A406), M427 (= M431), G451 (= G455), D452 (≠ E456), G453 (= G457), S454 (= S458), N479 (= N483), H481 (≠ G485), L482 (≠ Y486), G483 (≠ I487), M484 (≠ S488), V485 (≠ I489)

Query Sequence

>WP_013554960.1 NCBI__GCF_000186245.1:WP_013554960.1
MDGHIKKVSDIVAEFLAENEQIANDIFMVSGGGNMHLIDSIGRNERLNYICNHHEQACAI
AAEGYARVTNKVGVAMVTTGPGGTNAITGVYGAWVDSIPTLTISGQVKFETTVASQPELK
LRQLGDQEINIIDVVKPITKYAVMATDKYEILYHLQKAVYEAKSGRPGPVWVDIPLDIQG
ANVDEKRLKQFVPPAPPEYDLKVDEVVSLLKQAERPVIIAGNGITLSGANEKFLQLAEKL
GVPVVGTFARYDIVKTEHPLFFGRYGTVGHRMANFTVQNADLVLAIGARMNIRAISYNWE
FFAREAIKIAVDIDPAELRKHTLEIDVPIEADAKVFIEAMLERFENENMPKYDKWIEKCL
TYKKRYPTIEPTRQKIVDTVDSYNFFDLLSDMTADDTVFVFGNGTACVSSYQSLKVKGKQ
KVVVNSGCASMGYDLPAAIGAAFGSKSKQIVCVTGEGSLQMNLQELQTIIHHRLPVKLFV
LNNAGYISIRNTQNNFFKGHKVGSDASSGVSFPDTLKLAEAYGFRAFRLETQLDLVRQLE
NILRIEGPVICEVMLSPTEKMEPKLSSEVKPDGTMISKPLEDMFPFLDRKEFFENMIIEP
VDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory