SitesBLAST

O30126 O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoseryl-tRNA(Cys) synthetase; SepRS; EC 6.1.1.27 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
46% identity, 99% coverage: 4:529/531 of query aligns to 6:533/534 of O30126

query
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O30126

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N325 (= N317) binding substrate
E418 (= E410) mutation to N: Shows reduced phosphoserine ligation activity. Shows appreciable ligation activity with both suppressor tRNA(Opal) and tRNA(Amber), and reduces ligation activity with tRNA(Cys); when associated with N-420. Shows higher activity than the E418N/E420N mutant with both suppressor tRNA(Opal) and tRNA(Amber), and the activity with tRNA(Opal) and tRNA(Amber) is almost 30% of that of the wild-type SepRS with tRNA(Cys); when associated with N-420 and V-423.
E420 (= E412) mutation to N: Shows reduced phosphoserine ligation activity. Shows appreciable ligation activity with both suppressor tRNA(Opal) and tRNA(Amber), and reduces ligation activity with tRNA(Cys); when associated with N-418. Shows higher activity than the E418N/E420N mutant with both suppressor tRNA(Opal) and tRNA(Amber), and the activity with tRNA(Opal) and tRNA(Amber) is almost 30% of that of the wild-type SepRS with tRNA(Cys); when associated with N-418 and V-423.
T423 (= T415) mutation to V: Shows higher activity than the E418N/E420N mutant with both suppressor tRNA(Opal) and tRNA(Amber), and the activity with tRNA(Opal) and tRNA(Amber) is almost 30% of that of the wild-type SepRS with tRNA(Cys); when associated with N-418 and N-420.

2du4A Crystal structure of archaeoglobus fulgidus o-phosphoseryl-tRNA synthetase complexed with trnacys (see paper)
45% identity, 70% coverage: 160:529/531 of query aligns to 87:465/466 of 2du4A

query
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2du4A

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binding : E350 (= E410), E352 (= E412), T355 (= T415), P360 (= P420), A361 (= A421), N364 (= N424), G375 (= G435), R424 (≠ T488), D452 (= D516), R454 (= R518), G455 (= G519), P456 (= P520), F458 (= F522)

2du3B Crystal structure of archaeoglobus fulgidus o-phosphoseryl-tRNA synthetase complexed with trnacys and o-phosphoserine (see paper)
46% identity, 67% coverage: 174:529/531 of query aligns to 105:464/465 of 2du3B

query
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2du3B

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S

D

D

V

I

N

N

L

L

K

Y

L

I

E

H

E

E

V

N

A

V

L

R

N

R

Y

Y

I

I

T

L

S

W

N

K

K

G

L

K

I

I

D

D

I

V

R

R

G

G

P

P

I

L

F

F

S

V

T

T

V

V

T

K

F

A

E

E

V

I

binding phosphoserine: H117 (= H186), M118 (= M187), T119 (= T188), S162 (= S231), S164 (= S233), Y204 (= Y273), Y205 (= Y274), N256 (= N317), G258 (= G319)

P15625 Phenylalanine--tRNA ligase alpha subunit; Phenylalanyl-tRNA synthetase alpha subunit; PheRS; EC 6.1.1.20 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 57% coverage: 37:339/531 of query aligns to 220:480/503 of P15625

query
sites
P15625

I

V

Q

Q

M

I

R

S

T

S

G

G

Q

A

S

L

H

H

M

P

L

L

Y

N

N

K

T

V

I

R

W

E

E

E

L

F

R

R

Q

Q

A

I

Y

F

L

F

N

S

L

M

G

G

F

F

N

T

E

E

T

M

I

P

N

S

P

N

V

Q

F

Y

V

V

E

E

Q

T

D

G

D

-

I

-

Y

F

R

W

Q

N

F

F

G

-

P

-

E

-

A

-

P

-

A

-

V

-

L

-

D

D

R

A

C

L

F

Y

Y

V

L

P

A

Q

G

Q

L

H

P

P

R

A

P

R

D

D

I

L

G

-

I

-

G

-

M

Q

D

D

K

T

I

F

A

Y

V

I

I

K

E

D

E

P

I

L

G

T

V

A

E

D

I

L

N

P

D

D

K

K

-

T

-

Y

I

M

Q

D

A

N

L

I

K

K

E

A

V

V

F

H

R

E

G

Q

Y

G

K

R

K

F

G

G

D

-

E

-

S

-

G

-

D

-

L

-

V

-

H

-

V

-

S

-

V

-

A

-

L

-

D

-

V

-

Q

-

D

-

S

S

V

I

G

G

L

Y

R

R

I

Y

L

-

E

-

N

N

V

W

F

K

P

P

E

E

L

-

R

-

E

-

L

-

K

-

P

-

V

E

S

C

S

Q

R

K

T

L

T

V

L

L

R

R

S

T

H

H

M

S

T

T

S

A

G

-

W

-

F

-

L

I

T

S

L

A

Q

R

S

M

L

L

H

H

N

D

Q

L

S

A

K

K

-

D

-

P

L

K

P

P

L

T

K

R

L

L

F

F

S

S

I

I

D

D

R

R

C

V

F

F

R

R

R

N

E

E

Q

A

R

V

E

-

D

D

M

A

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S

Q

A

V

S

E

C

G

V

V

W

L

A

A

D

D

D

Y

E

N

I

I

S

T

L

L

D

G

M

D

G

L

M

I

A

K

V

F

S

M

E

E

N

E

L

F

Q

E

H

R

F

M

G

G

F

V

K

T

K

G

F

L

K

R

F

F

I

K

P

P

D

T

E

-

K

-

S

-

K

-

Y

-

Y

Y

I

N

P

P

G

Y

T

T

Q

E

-

P

-

S

T

M

E

E

V

I

Y

F

G

S

Y

W

H

H

P

E

K

G

L

L

N

Q

E

K

W

W

V

V

E

E

V

I

A

G

T

N

F

S

G

G

L

M

Y

F

S

R

P

P

I

E

A

M

L

L

S

E

H

S

Y

M

G

G

I

L

-

P

-

K

D

D

K

L

Q

R

V

V

M

L

N

G

L

W

G

G

V

L

G

S

A

L

E

E

R

R

I

P

A

T

M

M

I

I

L

K

H

Y

N

K

Q

V

T

Q

D

N

V

I

R

R

E

E

M

L

V

L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

3l4gC Crystal structure of homo sapiens cytoplasmic phenylalanyl-tRNA synthetase (see paper)
33% identity, 31% coverage: 178:339/531 of query aligns to 319:478/508 of 3l4gC

query
sites
3l4gC

S

A

S

R

R

K

T

N

T

L

L

L

R

R

S

T

H
|
H

M

T

T
|
T

S

S

G

A

W

S

F

A

L

R

T

A

L

L

Q

Y

S

R

L

L

H

A

N

Q

Q

K

S

K

K

P

L

F

-

T

P

P

L

V

K

K

L

Y

F

F

S

S

I

I

D

D

R

R

C

V

F

F

R
|
R

R

N

E

E

Q

T

R

L

E

-

D

D

M

A

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S
x
Q

A

I

S
x
E

C

G

V

V

W

V

A

A

D

D

D

H

E

G

I

L

S

T

L

L

D

G

M

H

G

L

M

M

A

G

V

V

S

L

E

R

N

E

L

F

Q

T

H

K

F

L

G

G

F

I

K

T

K

Q

F

L

K

R

F

F

I

K

P

P

D

-

E

-

K

-

S

-

K

-

Y

A

Y

Y

I

N

P

P

G

Y

T
|
T

Q

E

T

P

-

S

-

M

E

E

V

V

Y

F

G

S

Y

Y

H

H

P

Q

K

G

L

L

N

K

E

K

W

W

V

V

E

E

V

V

A

G

T
x
N

F
x
S

G

G

L

V

Y
x
F

S

R

P

P

I

E

A

M

L

L

S

L

H

P

Y

M

G

G

I

L

D

P

K

E

-

N

-

V

Q

S

V

V

M

I

N
x
A

L

W

G
|
G

V

L

G

S

A

L

E

E

R

R

I

P

A

T

M

M

I

I

L

K

H

Y

N

G

Q

I

T

N

D

N

V

I

R

R

E

E

M

L

V

V

active site: H327 (= H186), R358 (= R216), Q372 (≠ S231), T413 (= T278), A456 (≠ N317)
binding phenylalanine: T329 (= T188), Q372 (≠ S231), E374 (≠ S233), N434 (≠ T297), S435 (≠ F298), F438 (≠ Y301), G458 (= G319)

Sites not aligning to the query:

active site: 274

Q9Y285 Phenylalanine--tRNA ligase alpha subunit; CML33; Phenylalanyl-tRNA synthetase alpha subunit; PheRS; EC 6.1.1.20 from Homo sapiens (Human) (see 3 papers)
33% identity, 31% coverage: 178:339/531 of query aligns to 319:478/508 of Q9Y285

query
sites
Q9Y285

S

A

S

R

R

K

T

N

T

L

L

L

R

R

S

T

H

H

M

T

T
|
T

S

S

G

A

W

S

F

A

L

R

T

A

L

L

Q

Y

S

R

L

L

H

A

N

Q

Q

K

S

K

K

P

L

F

-

T

P

P

L

V

K

K

L

Y

F

F

S

S

I

I

D

D

R

R

C

V

F

F

R

R

R

N

E

E

Q

T

R

L

E

-

D

D

M

A

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S
x
Q

A
x
I

S
x
E

C

G

V

V

W

V

A

A

D

D

D

H

E

G

I

L

S

T

L

L

D

G

M

H

G

L

M

M

A

G

V

V

S

L

E

R

N

E

L

F

Q

T

H

K

F

L

G

G

F

I

K

T

K

Q

F

L

K

R

F

F

I

K

P

P

D

-

E

-

K

-

S

-

K

-

Y

A

Y

Y

I
x
N

P

P

G
x
Y

T

T

Q

E

T

P

-

S

-

M

E

E

V

V

Y

F

G

S

Y

Y

H

H

P

Q

K

G

L

L

N

K

E

K

W

W

V

V

E

E

V

V

A

G

T

N

F

S

G

G

L

V

Y
x
F

S

R

P

P

I

E

A

M

L

L

S

L

H

P

Y

M

G

G

I

L

D

P

K

E

-

N

-

V

Q

S

V

V

M

I

N

A

L

W

G

G

V

L

G

S

A

L

E

E

R

R

I

P

A

T

M

M

I

I

L

K

H

Y

N

G

Q

I

T

N

D

N

V

I

R

R

E

E

M

L

V

V

T329 (= T188) binding L-phenylalanine
QIE 372:374 (≠ SAS 231:233) binding L-phenylalanine
N410 (≠ I275) to K: in RILDBC2; uncertain significance; dbSNP:rs1971301248
Y412 (≠ G277) binding L-phenylalanine
F438 (≠ Y301) binding L-phenylalanine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
256 F → L: in RILDBC2; uncertain significance; dbSNP:rs941586004

7dpiC Plasmodium falciparum cytoplasmic phenylalanyl-tRNA synthetase in complex with brd7929 (see paper)
31% identity, 30% coverage: 178:338/531 of query aligns to 125:287/292 of 7dpiC

query
sites
7dpiC

S

S

S

K

R

K

T

N

T

V

L

L

R

R

S

T

H

H

M

T

T

T

S

A

G

N

W

S

F

C

L

R

T

A

L

L

Q

F

S

K

L

L

H

A

N

K

Q

E

S

Y

K

K

-

E

-

A

-

G

-

C

-

I

L

K

P

P

L

K

K

K

L

Y

F

F

S

S

I

I

D

D

R

R

C

V

F

F

R

R

R

N

E

E

Q

N

R

L

E

-

D

D

M

S

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S

Q

A

V

S

E

C

G

V

L

W

I

A

I

D

D

D

K

E

N

I

I

S

G

L

L

D

S

M

H

G

L

M

I

A

G

V

T

S

L

E

A

N

A

L

F

L

Y

Q

K

H

H

F

I

G

G

F

I

K

H

K

K

F

L

K

K

F

F

I

K

P

P

D

T

E

-

K

-

S

-

K

-

Y

-

Y

F

I

N

P

P

G

Y

T

T

Q

E

-

P

-

S

T

M

E

E

V

I

Y

Y

G

G

Y

Y

H

H

P

E

K

Q

L

S

N

K

E

K

W

W

V

L

E
|
E

V
|
V

A
x
G

T
x
N

F
x
S

G

G

L

I

Y

F

S

R

P

P

I

E

A

M

L

L

S

R

H

S

Y

M

G

G

I

F

D

S

K

E

Q

E

-

V

-

S

V

V

M

I

N

A

L
x
W

G
|
G

V
x
L

G
x
S

A

L

E

E

R
|
R

I
x
P

A

T

M

M

I

I

L

K

H

Y

N

N

Q

I

T

K

D

N

V

I

R

R

E

D

M

L

binding (8R,9S,10S)-10-[(dimethylamino)methyl]-N-(4-methoxyphenyl)-9-[4-(2-phenylethynyl)phenyl]-1,6-diazabicyclo[6.2.0]decane-6-carboxamide: E241 (= E294), V242 (= V295), G243 (≠ A296), N244 (≠ T297), S245 (≠ F298), W267 (≠ L318), G268 (= G319), L269 (≠ V320), S270 (≠ G321), R273 (= R324), P274 (≠ I325)

A5K9S0 Phenylalanine--tRNA ligase alpha subunit; Phenylalanine tRNA-synthetase alpha subunit; FRS; PheRS; Phenylalanyl-tRNA synthetase alpha subunit; EC 6.1.1.20 from Plasmodium vivax (strain Salvador I) (see paper)
30% identity, 31% coverage: 173:338/531 of query aligns to 396:562/569 of A5K9S0

query
sites
A5K9S0

E

E

L

L

K

K

P

S

V

-

S

T

S

K

R

K

T

N

T

V

L

L

R

R

S

T

H

H

M

T

T

T

S

A

G

N

W

S

F

C

L

R

T

A

L

L

Q

F

S

Q

L

L

H

A

N

K

Q

E

-

Y

-

Q

-

K

-

T

-

G

S

S

K

I

L

I

P

P

L

K

K

K

L

F

F

F

S

S

I

I

D

D

R

R

C

V

F

F

R

R

R

N

E

E

Q

N

R

L

E

-

D

D

M

S

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S

Q

A

V

S

E

C

G

V

L

W

I

A

I

D

D

D

R

E

N

I

L

S

G

L

L

D

S

M

H

G

L

M

I

A

G

V

T

S

L

E

S

N

A

L

F

L

Y

Q

K

H

Y

F

I

G

G

F

I

K

S

K

K

F

L

K

R

F

F

I

K

P

P

D

T

E

-

K

-

S

-

K

-

Y

-

Y

F

I

N

P

P

G

Y

T

T

Q
x
E

-

P

-

S

T

M

E

E

V

V

Y

Y

G

G

Y

Y

H

H

P

E

K

E

L

N

N

K

E

K

W

W

V

L

E

E

V

V

A

G

T

N

F

S

G

G

L

I

Y

F

S

R

P

P

I

E

A

M

L

L

S

R

H

A

Y

M

G

G

I

F

-

P

D

E

K

V

Q

S

V

V

M

I

N

A

L

W

G

G

V

L

G

S

A

L

E

E

R

R

I

P

A

T

M

M

I

I

L

K

H

Y

N

S

Q

I

T

R

D

N

V

I

R

R

E

D

M

L

E499 (≠ Q279) binding Mg(2+)

Q9W3J5 Phenylalanine--tRNA ligase alpha subunit; Phenylalanyl-tRNA synthetase alpha subunit; PheRS; EC 6.1.1.20 from Drosophila melanogaster (Fruit fly) (see paper)
30% identity, 31% coverage: 178:342/531 of query aligns to 318:481/498 of Q9W3J5

query
sites
Q9W3J5

S

A

S

Q

R

K

T

N

T

L

L

L

R

R

S

T

H

H

M

T

T

T

S

A

G

V

W

S

F

A

L

R

T

M

L

L

Q

Y

S

K

L

L

H

A

N

N

Q

Q

S

E

K

G

-

G

-

F

L

K

P

A

L

A

K

K

L

Y

F

F

S

S

I

I

D

D

R

K

C

V

F

F

R

R

R

N

E

E

Q

T

R

L

E

-

D

D

M

A

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S

Q

A

V

S

E

C

G

V

V

W

I

A

A

D

D

D

V

E

G

I

L

S

T

L

L

D

G

M

D

G

L

M

I

A

G

V

T

S

L

E

Y

N

E

L

F

Q

R

H

K

F

L

G

G

F

I

K

T

K

Q

F

L

K

E

F

F

I

K

P

P

D

-

E

-

K

-

S

-

K

-

Y

A

Y

Y

I

N

P

P

G

Y

T

T

Q

E

-

P

-

S

T

M

E

E

V

I

Y

F

G

C

Y

Y

H

H

P

P

K

G

L

L

N

A

E

K

W

W

V

I

E

E

V

V

A

G

T

N

F

S

G

G

L

V

Y

F

S

R

P

P

I

E

A

M

L

L

S

L

H

P

Y

M

G

G

I

L

D

P

K

E

-

N

-

V

Q

N

V

V

M

I

N
x
A

L

W

G

G

V

L

G

S

A

L

E

E

R

R

I

P

A

T

M

M

I

I

L

K

H

Y

N

G

Q

I

T

N

D

N

V

I

R

R

E

D

M

L

V

V

Y

G

P

P

Q

K

A456 (≠ N317) mutation to G: Decreased aminoacylation, increased misacylation of non-cognate Tyr, increased amino acid misincorporation, ommatidia defects, neurodegeneration, impaired locomotive performance, reduced lifespan, smaller organ size due to reduced cell proliferation and increased ER stress. Similar but stronger phenotypes except that smaller organ size is due primarily to apoptosis; when associated with 'W-158' in beta subunit.

7by6A Plasmodium vivax cytoplasmic phenylalanyl-tRNA synthetase in complex with brd1389 (see paper)
30% identity, 31% coverage: 173:338/531 of query aligns to 126:292/299 of 7by6A

query
sites
7by6A

E

E

L

L

K

K

P

S

V

-

S

T

S

K

R

K

T

N

T

V

L

L

R

R

S

T

H

H

M

T

T

T

S

A

G

N

W

S

F

C

L

R

T

A

L

L

Q

F

S

Q

L

L

H

A

N

K

Q

E

-

Y

-

Q

-

K

-

T

-

G

S

S

K

I

L

I

P

P

L

K

K

K

L

F

F

F

S

S

I

I

D

D

R

R

C

V

F

F

R

R

R

N

E

E

Q

N

R

L

E

-

D

D

M

S

S

T

H

H

L

L

M

A

T

E

Y

F

H

H

S

Q

A

V

S

E

C

G

V

L

W

I

A

I

D

D

D

R

E

N

I

L

S

G

L

L

D

S

M

H

G

L

M

I

A

G

V

T

S

L

E

S

N

A

L

F

L

Y

Q

K

H

Y

F
x
I

G

G

F

I

K

S

K

K

F

L

K

R

F

F

I

K

P

P

D

T

E

-

K

-

S

-

K

-

Y

-

Y

F

I

N

P

P

G

Y

T

T

Q

E

-

P

-

S

T

M

E

E

V

V

Y

Y

G

G

Y

Y

H

H

P

E

K

E

L

N

N

K

E

K

W

W

V

L

E
|
E

V
|
V

A

G

T
x
N

F
x
S

G

G

L

I

Y

F

S

R

P

P

I

E

A

M

L

L

S

R

H

A

Y

M

G

G

I

F

-

P

D

E

K

V

Q

S

V

V

M

I

N

A

L

W

G
|
G

V
x
L

G
x
S

A

L

E

E

R
|
R

I
x
P

A

T

M

M

I

I

L

K

H

Y

N

S

Q

I

T

R

D

N

V

I

R

R

E

D

M

L

binding (3S,4R,8R,9R,10S)-N-(4-cyclopropyloxyphenyl)-10-(methoxymethyl)-3,4-bis(oxidanyl)-9-[4-(2-phenylethynyl)phenyl]-1,6-diazabicyclo[6.2.0]decane-6-carboxamide: I213 (≠ F257), E246 (= E294), V247 (= V295), N249 (≠ T297), S250 (≠ F298), G273 (= G319), L274 (≠ V320), S275 (≠ G321), R278 (= R324), P279 (≠ I325)

6p26A Escherichia coli tRNA synthetase in complex with compound 1 (see paper)
23% identity, 29% coverage: 184:338/531 of query aligns to 66:229/241 of 6p26A

query
sites
6p26A

R

R

S

A

H

D

M

H

T

D

S

T

G

F

W

W

F

F

L

D

T

T

L

T

Q

R

S

L

L

L

H

R

N

T

Q

Q

S

T

-

S

-

G

-

V

-

Q

-

I

-

R

-

T

-

M

-

K

-

A

-

Q

K

Q

L

P

P

P

L

I

K

R

L

I

F

I

S

A

I

P

D

G

R

R

C

V

F

Y

R

R

R

N

E

D

Q

Y

R

-

E

-

D

D

M

Q

S

T

H

H

L

T

M

P

T

M

Y

F

H

H

S

Q

A

M

S
x
E

C

G

V

L

W

I

A

V

D

D

D

T

E

N

I

I

S

S

L

F

D

T

M

N

G

L

M

K

A

G

V

T

S

L

E

H

N

D

L

F

L

L

Q

R

H

N

F

F

G

F

F

E

K

E

K

D

F

L

K

Q

F

I

I

R

P

F

D

R

E

P

K

S

K

Y

S
x
F

K

P

Y
x
F

Y

T

I

E

P

P

G

S

T

A

Q

E

T

V

E

D

V

V

Y

M

G

G

Y

-

H

-

P

-

K

K

L

N

N

G

E

K

W

W

V

L

E

E

V

V

A

L

T
x
G

F
x
C

G

G

L

M

Y
x
V

S

H

P

P

I

N

A

V

L

L

S

R

H

N

Y

V

G

G

I

I

D

D

K

P

Q

E

V

V

M

Y

N

S

-

G

-

F

-
x
A

L
x
F

G

G

V

M

G

G

A

M

E

E

R

R

I

L

A

T

M

M

I

L

L

R

H

Y

N

G

Q

V

T

T

D

D

V

L

R

R

E

S

M

F

binding N-benzyl-2-(cyclohex-1-en-1-yl)ethan-1-amine: E124 (≠ S233), F162 (≠ S271), F164 (≠ Y273), G185 (≠ T297), C186 (≠ F298), V189 (≠ Y301), A208 (vs. gap), F209 (≠ L318)

Sites not aligning to the query:

binding N-benzyl-2-(cyclohex-1-en-1-yl)ethan-1-amine: 57

6p24A Escherichia coli tRNA synthetase (see paper)
23% identity, 29% coverage: 184:338/531 of query aligns to 66:229/241 of 6p24A

query
sites
6p24A

R

R

S

A

H

D

M

H

T

D

S

T

G

F

W

W

F

F

L

D

T

T

L

T

Q

R

S

L

L

L

H

R

N

T

Q

Q

S

T

-

S

-

G

-

V

-

Q

-

I

-

R

-

T

-

M

-

K

-

A

-

Q

K

Q

L

P

P

P

L

I

K

R

L

I

F

I

S

A

I

P

D

G

R

R

C

V

F

Y

R

R

R

N

E

D

Q

Y

R

-

E

-

D

D

M

Q

S

T

H

H

L

T

M

P

T

M

Y

F

H

H

S

Q

A

M

S

E

C

G

V

L

W

I

A

V

D

D

D

T

E

N

I

I

S

S

L

F

D

T

M

N

G

L

M

K

A

G

V

T

S

L

E

H

N

D

L

F

L

L

Q

R

H

N

F

F

G

F

F

E

K

E

K

D

F

L

K

Q

F

I

I

R

P

F

D

R

E

P

K

S

K

Y

S

F

K

P

Y

F

Y

T

I

E

P

P

G

S

T

A

Q
x
E

T

V

E

D

V

V

Y

M

G

G

Y

-

H

-

P

-

K

K

L

N

N

G

E

K

W

W

V

L

E
|
E

V

V

A

L

T

G

F

C

G

G

L

M

Y

V

S

H

P

P

I

N

A

V

L

L

S

R

H

N

Y

V

G

G

I

I

D

D

K

P

Q

E

V

V

M

Y

N

S

-

G

-

F

-

A

L

F

G

G

V

M

G

G

A

M

E

E

R

R

I

L

A

T

M

M

I

L

L

R

H

Y

N

G

Q

V

T

T

D

D

V

L

R

R

E

S

M

F

binding magnesium ion: E170 (≠ Q279), E182 (= E294)

Sites not aligning to the query:

binding magnesium ion: 16, 17

6oz5A Escherichia coli tRNA synthetase in complex with compound 3 (see paper)
23% identity, 29% coverage: 184:338/531 of query aligns to 66:229/241 of 6oz5A

query
sites
6oz5A

R

R

S

A

H

D

M

H

T

D

S

T

G

F

W

W

F

F

L

D

T

T

L

T

Q

R

S

L

L

L

H

R

N

T

Q
|
Q

S

T

-

S

-

G

-

V

-
x
Q

-

I

-

R

-

T

-

M

-

K

-

A

-

Q

K

Q

L

P

P

P

L

I

K

R

L

I

F

I

S

A

I

P

D

G

R

R

C

V

F

Y

R

R

R

N

E

D

Q

Y

R

-

E

-

D

D

M

Q

S

T

H

H

L

T

M

P

T

M

Y

F

H

H

S

Q

A

M

S
x
E

C

G

V

L

W

I

A

V

D

D

D

T

E

N

I

I

S

S

L

F

D

T

M

N

G

L

M

K

A

G

V

T

S

L

E

H

N

D

L

F

L

L

Q

R

H

N

F

F

G

F

F

E

K

E

K

D

F

L

K

Q

F

I

I

R

P

F

D

R

E

P

K

S

K

Y

S
x
F

K

P

Y
x
F

Y

T

I

E

P

P

G

S

T

A

Q

E

T

V

E

D

V

V

Y

M

G

G

Y

-

H

-

P

-

K

K

L

N

N

G

E

K

W

W

V

L

E

E

V

V

A

L

T
x
G

F
x
C

G

G

L

M

Y

V

S

H

P

P

I

N

A

V

L

L

S

R

H

N

Y

V

G

G

I

I

D

D

K

P

Q

E

V

V

M

Y

N

S

-

G

-

F

-
x
A

L
x
F

G
|
G

V

M

G

G

A

M

E

E

R

R

I

L

A

T

M

M

I

L

L

R

H

Y

N

G

Q

V

T

T

D

D

V

L

R

R

E

S

M

F

binding 2-({[(2S)-1-cyclohexylpropan-2-yl]amino}methyl)phenol: Q83 (= Q201), Q88 (vs. gap), E124 (≠ S233), F162 (≠ S271), F164 (≠ Y273), G185 (≠ T297), C186 (≠ F298), A208 (vs. gap), F209 (≠ L318), G210 (= G319)

3pcoC Crystal structure of e. Coli phenylalanine-tRNA synthetase complexed with phenylalanine and amp (see paper)
23% identity, 29% coverage: 184:336/531 of query aligns to 148:309/323 of 3pcoC

query
sites
3pcoC

R

R

S

A

H

D

M
x
H

T

D

S

T

G

F

W

W

F

F

L

D

T

T

L

T

Q

R

S

L

L

L

H

R

N

T

Q
|
Q

S

T

-

S

-

G

-

V

-

Q

-

I

-

R

-

T

-

M

-

K

-

A

-

Q

K

Q

L

P

P

P

L

I

K

R

L

I

F

I

S

A

I

P

D

G

R

R

C

V

F

Y

R
|
R

R

N

E

D

Q

Y

R

-

E

-

D

D

M

Q

S
x
T

H
|
H

L

T

M

P

T

M

Y
x
F

H

H

S
x
Q

A

M

S

E

C

G

V

L

W

I

A

V

D

D

D

T

E

N

I

I

S

S

L

F

D

T

M

N

G

L

M

K

A

G

V

T

S

L

E

H

N

D

L

F

L

L

Q

R

H

N

F

F

G

F

F

E

K

E

K

D

F

L

K

Q

F

I

I

R

P

F

D

R

E

P

K

S

K

Y

S
x
F

K

P

Y
x
F

Y
x
T

I

E

P

P

G

S

T

A

Q

E

T

V

E

D

V

V

Y

M

G

G

Y

-

H

-

P

-

K

K

L

N

N

G

E

K

W

W

V

L

E
|
E

V

V

A

L

T
x
G

F
x
C

G

G

L

M

Y

V

S

H

P

P

I

N

A

V

L

L

S

R

H

N

Y

V

G

G

I

I

D

D

K

P

Q

E

V

V

M

Y

N

S

-

G

-

F

-
x
A

L
x
F

G
|
G

V

M

G

G

A

M

E

E

R
|
R

I

L

A

T

M

M

I

L

L

R

H

Y

N

G

Q

V

T

T

D

D

V

L

R

R

active site: H151 (≠ M187), Q165 (= Q201), R191 (= R216), Q204 (≠ S231), T247 (≠ Y274), A290 (vs. gap)
binding adenosine monophosphate: R191 (= R216), T197 (≠ S224), H198 (= H225), F202 (≠ Y229), E264 (= E294), R297 (= R324)
binding phenylalanine: Q165 (= Q201), F244 (≠ S271), F246 (≠ Y273), G267 (≠ T297), C268 (≠ F298), A290 (vs. gap), F291 (≠ L318), G292 (= G319)

4p72D Phers in complex with compound 2a (see paper)
23% identity, 38% coverage: 134:336/531 of query aligns to 38:230/244 of 4p72D

query
sites
4p72D

G

G

Y

Y

K

E

-

V

-

A

K

E

G

G

D

P

E

E

S

V

G

E

D

D

L

-

V

-

H

Y

H

H

V

N

S

F

V

E

A

A

L
|
L

D

N

V

I

Q

P

D

G

S

H

V

H

G

P

L

A

R

R

I

A

L

M

E
x
H

N

D

V

T

F

F

P

Y

E

-

L

-

R

-

E

-

L

-

K

-

P

-

V

F

S

N

S

A

R

N

T

M

T

L

L

L

R

R

S

T

H
|
H

M

T

T

S

S

P

G

V

W

Q

F

V

L

R

T

T

L

M

Q

E

S

S

L

-

H

-

N

-

Q

-

S

Q

K

Q

L

P

P

P

L

I

K

R

L

I

F

V

S

C

I

P

D

G

R

R

C

V

F

Y

R
|
R

R

C

E

D

Q

S

R

-

E

-

D

D

M

L

S

T

H

H

L

S

M

P

T

M

Y

F

H

H

S
x
Q

A

V

S
x
E

C

G

V

L

W

L

A

V

D

D

D

E

E

G

I

V

S

S

L

F

D

A

M

D

G

L

M

K

A

G

V

T

S

I

E

E

N

E

L

F

L

L

Q

R

H

A

F

F

G

F

F

E

K

K

K

Q

F

L

K

E

F

V

I

R

P

F

D

R

E

P

K

S

K

F

S

F

K

P

Y
x
F

Y
x
T

I

E

P

P

G

S

T

A

Q

E

T

V

E

D

V

I

Y

Q

G

C

Y

V

H

I

P

C

K

S

L

G

N

N

E

G

W

W

V

L

E

E

V

V

A

M

T
x
G

F
x
C

G

G

L

M

Y

V

S

H

P

P

I

N

A

V

L

L

S

R

H

M

Y

S

G

N

I

I

D

D

K

P

-

E

-

K

-

F

Q

Q

V

G

M

F

N
x
A

L
x
F

G
|
G

V
x
M

G

G

A

A

E

E

R

R

I

L

A

A

M

M

I

L

L

R

H

Y

N

G

Q

V

T

N

D

D

V

L

R

R

active site: H69 (≠ E165), H83 (= H186), R109 (= R216), Q122 (≠ S231), T165 (≠ Y274), A211 (≠ N317)
binding 2-{3-[(4-chloropyridin-2-yl)amino]phenoxy}-N-methylacetamide: L57 (= L153), E124 (≠ S233), F164 (≠ Y273), T165 (≠ Y274), G188 (≠ T297), C189 (≠ F298), A211 (≠ N317), F212 (≠ L318), G213 (= G319), M214 (≠ V320)

Query Sequence

>WP_013643648.1 NCBI__GCF_000191585.1:WP_013643648.1
MKTNKLLKLARKDFEKAWVETAQTLKKPHTDNEYPRIQMRTGQSHMLYNTIWELRQAYLN
LGFNETINPVFVEQDDIYRQFGPEAPAVLDRCFYLAGLPRPDIGIGMDKIAVIEEIGVEI
NDDKIQALKEVFRGYKKGDESGDDLVHHVSVALDVQDSVGLRILENVFPELRELKPVSSR
TTLRSHMTSGWFLTLQSLHNQSKLPLKLFSIDRCFRREQREDMSHLMTYHSASCVWADDE
ISLDMGMAVSENLLQHFGFKKFKFIPDEKKSKYYIPGTQTEVYGYHPKLNEWVEVATFGL
YSPIALSHYGIDKQVMNLGVGAERIAMILHNQTDVREMVYPQTYAKWHLTDRELASMLHI
NYYPFSTDGRELMDILKQKFLEYSEKESPCEFTVFEGEFLGRNIRVKILEPESNTRLLGP
AAWNSIYIKDGNIVGLPENNAKDELSLETVQNGIPLGITYMDGVIAKACYMIEEMIVGGE
TESEFRTTLAKSLSDVNLKLEEVALNYITSNNKLIDIRGPIFSTVTFEVID

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory