SitesBLAST
Comparing WP_013644215.1 NCBI__GCF_000191585.1:WP_013644215.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
58% identity, 83% coverage: 45:377/399 of query aligns to 4:327/350 of 6nmxA
- active site: K60 (= K100), T84 (= T124), E216 (≠ Q258), S220 (= S262), A238 (= A288), T315 (= T365)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K100), S83 (= S123), T84 (= T124), N86 (= N126), T87 (= T127), F133 (= F173), N153 (= N193), S154 (= S194), R159 (= R199), V185 (= V226), G186 (= G227), N187 (= N228), A188 (= A229), G189 (= G230), N190 (= N231), A238 (= A288), I239 (= I289), E285 (= E335), T315 (= T365)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
58% identity, 83% coverage: 45:377/399 of query aligns to 2:325/345 of 6cgqB
- active site: K58 (= K100), T82 (= T124), E214 (≠ Q258), S218 (= S262), A236 (= A288), T313 (= T365)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K100), S81 (= S123), T82 (= T124), N84 (= N126), T85 (= T127), V183 (= V226), G184 (= G227), N185 (= N228), A186 (= A229), N188 (= N231), A236 (= A288), I237 (= I289), E283 (= E335), T313 (= T365)
- binding phosphate ion: K58 (= K100), T85 (= T127), N151 (= N193), S152 (= S194), R157 (= R199), N185 (= N228)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
57% identity, 83% coverage: 48:377/399 of query aligns to 3:317/339 of 6cgqA
- active site: K56 (= K100), T80 (= T124), E206 (≠ Q258), S210 (= S262), A228 (= A288), T305 (= T365)
- binding pyridoxal-5'-phosphate: F55 (= F99), K56 (= K100), N82 (= N126), V175 (= V226), G176 (= G227), N177 (= N228), A178 (= A229), G179 (= G230), N180 (= N231), A228 (= A288), E275 (= E335), T305 (= T365), G306 (= G366)
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
54% identity, 87% coverage: 50:396/399 of query aligns to 8:348/350 of 2zsjA
- active site: K61 (= K100), T85 (= T124), Q218 (= Q258), A222 (≠ S262), A240 (= A288), T317 (= T365)
- binding pyridoxal-5'-phosphate: F60 (= F99), K61 (= K100), N87 (= N126), V186 (= V226), G187 (= G227), N188 (= N228), A189 (= A229), G190 (= G230), N191 (= N231), A240 (= A288), T317 (= T365), G318 (= G366)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
55% identity, 84% coverage: 51:386/399 of query aligns to 19:349/360 of A0R220
- K151 (≠ S182) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
55% identity, 82% coverage: 51:376/399 of query aligns to 19:337/360 of P9WG59
- K69 (= K100) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N126) binding pyridoxal 5'-phosphate
- K151 (≠ S182) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (= GNAGN 227:231) binding pyridoxal 5'-phosphate
- T326 (= T365) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
55% identity, 82% coverage: 51:376/399 of query aligns to 10:328/349 of 2d1fA
- active site: K60 (= K100), T84 (= T124), D209 (= D249), R213 (≠ M253), L215 (≠ T255), A240 (= A288), T317 (= T365)
- binding pyridoxal-5'-phosphate: F59 (= F99), K60 (= K100), N86 (= N126), V186 (= V226), G187 (= G227), N188 (= N228), A189 (= A229), G190 (= G230), N191 (= N231), A240 (= A288), T317 (= T365)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
51% identity, 87% coverage: 44:390/399 of query aligns to 2:342/350 of 1uimA
- active site: K61 (= K100), T85 (= T124), P212 (= P252), G216 (= G256), Q218 (= Q258), A240 (= A288), T317 (= T365)
- binding pyridoxal-5'-phosphate: F60 (= F99), K61 (= K100), N87 (= N126), G187 (= G227), N188 (= N228), A189 (= A229), G190 (= G230), N191 (= N231), A240 (= A288), E287 (= E335), T317 (= T365), G318 (= G366)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
51% identity, 87% coverage: 44:390/399 of query aligns to 2:342/351 of 3aexA
- active site: K61 (= K100), T85 (= T124), P212 (= P252), G216 (= G256), Q218 (= Q258), A240 (= A288), T317 (= T365)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K100), S84 (= S123), T85 (= T124), N87 (= N126), T88 (= T127), V186 (= V226), G187 (= G227), N188 (= N228), A189 (= A229), G190 (= G230), N191 (= N231), A240 (= A288), I241 (= I289), E287 (= E335), T317 (= T365)
- binding phosphate ion: K61 (= K100), T88 (= T127), N154 (= N193), S155 (= S194), R160 (= R199), N188 (= N228)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
51% identity, 87% coverage: 44:390/399 of query aligns to 2:342/351 of 1v7cA
- active site: K61 (= K100), T85 (= T124), P212 (= P252), G216 (= G256), Q218 (= Q258), A240 (= A288), T317 (= T365)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K100), S84 (= S123), T85 (= T124), N87 (= N126), T88 (= T127), F134 (= F173), N154 (= N193), S155 (= S194), R160 (= R199), V186 (= V226), G187 (= G227), N188 (= N228), A189 (= A229), G190 (= G230), N191 (= N231), A240 (= A288), I241 (= I289), E287 (= E335), T317 (= T365)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
51% identity, 87% coverage: 44:390/399 of query aligns to 1:341/350 of 3aeyA
- active site: K60 (= K100), T84 (= T124), P211 (= P252), G215 (= G256), Q217 (= Q258), A239 (= A288), T316 (= T365)
- binding sulfate ion: K60 (= K100), K60 (= K100), G85 (= G125), N86 (= N126), T87 (= T127), T87 (= T127), S154 (= S194), R159 (= R199), N187 (= N228), R228 (≠ E277), V230 (= V279), E231 (≠ K280), R232 (≠ D281), A239 (= A288)
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
36% identity, 92% coverage: 9:377/399 of query aligns to 19:409/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F99), K128 (= K100), D159 (≠ N126), G259 (≠ V226), G260 (= G227), N261 (= N228), L262 (≠ A229), G263 (= G230), N264 (= N231), A321 (= A288), H369 (≠ A337), T397 (= T365)
- binding s-adenosylmethionine: S64 (= S46), T65 (= T47), W66 (vs. gap), P67 (vs. gap), G69 (vs. gap), S90 (= S64), F92 (≠ E66), N97 (≠ P71), L98 (≠ F72), W100 (≠ K74), W115 (≠ Y87), W115 (≠ Y87), Q246 (≠ D213), F247 (≠ L214)
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 92% coverage: 9:377/399 of query aligns to 94:484/526 of Q9S7B5