SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013644575.1 NCBI__GCF_000191585.1:WP_013644575.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

7bkbB Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
25% identity, 86% coverage: 3:208/239 of query aligns to 6:243/296 of 7bkbB

query
sites
7bkbB

Y

F

F

F

K

L

G
|
G

C
|
C

V

I

V

A

R

P

E

N

K

R

L

Y

P

P

G

G

V

C

A

E

Q

A

A

S

T

A

E

I

K

K

I

T

L

S

K

E

K

K

A

V

G

G

V

I

D

K

Y

L

T

L

L

P

L

L

G

K

N

G

E

A

S

S

C
|
C

-

P

-

A

-

P

-

G

-

A

-

F

G

G

S

S

F

I

L

D

M

L

R

N

T

V

G

W

F

Y

K

A

D

M

E

A

A

A

E

R

E

N

V

L

M

-

K

-

N

-

L

-

Q

-

D

-

I

V

L

L

A

A

A

E

N

E

-

M

E

K

K

K

T

D

I

I

L

A

V

L

S

I

C
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C

S

N

G

G

C
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C

Y

Y

N

K

T

S

L

I

K

W

-

E

-

V

-

N

-

H

-

I

-

L

-

K

-

H

-

N

-

D

-

E

-

L

-

R

-

D

-

N

-

V

N

N

D

E

Y

V

K

L

A

A

L

E

F

I

D

D

V

M

E

Q

-

F

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K

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G

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I

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D

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V

I

W

H

H

T

L

S

A

E

E

L

L

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Y

K

Y

E

D

-

D

-

K

-

V

-

C

-

G

-

V

L

Q

L

K

V

I

K

K

N

D

K

S

I

V

H

T

V

T

N

P

S

L

T

S

E

G

K

A

T

K

V

V

T

A

Y

A

H

H

D

Y

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x
G

C
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C

H

H

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L

-

M

-

K

-

P

-

K

G

E

R

R

H

H

S

F

G

G

I

D

Y

T

E

E

E

N

P

P

R

M

D

W

A

F

I

E

K

E

A

L

S

I

A

G

D

A

L

L

-

G

V

A

E

E

M

P

K

I

R

Q

N

Y

R

R

E

N

R

K

S

M

R

Q

C
|
C

G

G

A

A

G

G

S

G

G

G

V

V

K

R

S

G

A

Y

F

D

P

I

E

V

L

H

A

A

L

L

E

D

V

I

G

T

C

N

R

E

R

K

I

L

Q

I

D

N

A

I

E

Q

E

E

V

A

G

G

A

A

D

D

I

A

L

I

V

T

T

E

T

L

C
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C

S

P

F
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F

C
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C

I

Q

L

L

N

Q

L

F

E

D

binding Non-cubane [4Fe-4S]-cluster: G9 (= G6), C10 (= C7), C42 (= C39), C43 (= C40), C79 (= C76), C82 (= C79), G156 (≠ P127), C157 (= C128), C197 (= C162), C198 (= C163), C235 (= C200), F237 (= F202), F237 (= F202), C238 (= C203)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
26% identity, 99% coverage: 3:238/239 of query aligns to 5:271/291 of 5odqB

query
sites
5odqB

Y

F

F

F

K

L

G
|
G

C
|
C

V

I

V

M

R

P

E

N

K

R

L

Y

P

A

G

G

V

V

A

E

Q

A

A

A

T

T

E

R

K

T

I

V

L

M

K

E

K

K

A

L

G

G

V

V

D

E

Y

L

T

V

L

D

L

M

G

T

N

G

E

A

S

S

C
|
C

-

P

-

A

-

P

-
x
G

-

V

-

F

G

G

S

S

F

F

L

D

M

Q

R

K

T

T

G

W

F

L

K

T

D

L

E

A

A

A

E

-

V

-

M

-

K

-

N

-

L

-

Q

R

D

N

I

L

L

C

A

I

A

A

N

E

E

E

K

M

-

G

-

V

T

D

I

I

L

V

V

T

S

V

C
|
C

S

N

G
|
G

C
|
C

Y

Y

N

G

T

S

L

L

K

F

N

E

D

A

Y

A

K

H

A

L

L

L

F

H

D

D

V

N

E

K

-

E

-

A

-

L

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N

-

F

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V

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N

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E

-

K

-

L

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D

-

K

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V

-

G

-

K

-

E

-

Y

-

K

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G

-

N

L

V

D

K

V

V

I

R

H

H

T

F

S

A

E

E

L

L

V

I

K

Y

E

N

L

D

L

I

V

G

K

V

N

D

K

K

I

I

H

-

V

A

N

E

S

K

T

V

E

E

K

R

T

P

-

L

-

N

-

I

-

N

V

V

T

G

Y

V

H

H

D

Y

P
x
G

C
|
C

H
|
H

L

F

-

L

-

K

-

P

-

T

-

D

G

V

R

K

H

H

S

L

G

G

I

S

Y

A

E

E

E

R

P

P

-

V

-

M

R

L

D

D

A

E

I

I

K

-

A

V

S

E

A

A

D

T

L

G

V

A

E

K

M

S

K

V

R

P

N

Y

R

A

E

D

R

K

S

M

R

M

C
|
C

G

G

A

A

G
|
G

S

G

G

G

V

V

K
x
R

S

A

A

R

F

E

P

L

E

E

L

L

A

S

L

L

E

D

V

M

G

T

C

N

R

E

R

K

I

I

Q

E

D

N

A

M

E

I

E

K

V

A

G

G

A

A

D

D

I

C

L

T

V

V

T

N

T

V

C
|
C

S

P

F
|
F

C
|
C

I

H

L
|
L

N

Q

L

F

E

D

N

R

A

G

L

Q

E

I

K

E

T

I

K

K

N

E

Q

K

G

F

E

G

N

K

G

E

S

Y

V

N

E

F

R

P

V

V

M

L

D

H

L

L

T

S

E

Q

L

L

-

L

-

G

I

L

L

A

M

M

G

G

M

M

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G6), C9 (= C7), C41 (= C39), C42 (= C40), C78 (= C76), G80 (= G78), C81 (= C79), G152 (≠ P127), C153 (= C128), H154 (= H129), C193 (= C162), C194 (= C163), C231 (= C200), F233 (= F202), C234 (= C203)
binding 2-bromanylethanesulfonic acid: G46 (vs. gap), G80 (= G78), H154 (= H129), G197 (= G166), R201 (≠ K170), F233 (= F202), L236 (= L205)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
26% identity, 99% coverage: 3:238/239 of query aligns to 5:271/291 of 5odhH

query
sites
5odhH

Y

F

F

F

K

L

G
|
G

C
|
C

V

I

V

M

R

P

E

N

K

R

L

Y

P

A

G

G

V

V

A

E

Q

A

A

A

T

T

E

R

K

T

I

V

L

M

K

E

K

K

A

L

G

G

V

V

D

E

Y

L

T

V

L

D

L

M

G

T

N

G

E

A

S

S

C
|
C

-

P

-
x
A

-
x
P

-
x
G

-

V

-

F

G

G

S

S

F

F

L

D

M

Q

R

K

T

T

G

W

F

L

K

T

D

L

E

A

A

A

E

-

V

-

M

-

K

-

N

-

L

-

Q

R

D

N

I

L

L

C

A

I

A

A

N

E

E

E

K

M

-

G

-

V

T

D

I

I

L

V

V

T

S

V

C
|
C

S

N

G
|
G

C
|
C

Y

Y

N

G

T

S

L

L

K

F

N

E

D

A

Y

A

K

H

A

L

L

L

F

H

D

D

V

N

E

K

-

E

-

A

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L

-

N

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F

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V

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N

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E

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K

-

L

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D

-

K

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V

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G

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K

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E

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Y

-

K

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G

-

N

L

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D

K

V

V

I

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H

T

F

S

A

E

E

L

L

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I

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Y

E

N

L

D

L

I

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G

K

V

N

D

K

K

I

I

H

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V

A

N

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E

K

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P

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L

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N

-

I

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N

V

V

T

G

Y

V

H

H

D

Y

P

G

C
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C

H
|
H

L

F

-

L

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K

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P

-

T

-

D

G

V

R

K

H

H

S

L

G

G

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S

Y

A

E

E

E

R

P

P

-

V

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M

R

L

D

D

A

E

I

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K

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A

V

S

E

A

A

D

T

L

G

V

A

E

K

M

S

K

V

R

P

N

Y

R

A

E

D

R

K

S

M

R

M

C
|
C

G

G

A

A

G
|
G

S
x
G

G

G

V

V

K
x
R

S

A

A

R

F

E

P

L

E

E

L

L

A

S

L

L

E

D

V

M

G

T

C

N

R

E

R

K

I

I

Q

E

D

N

A

M

E

I

E

K

V

A

G

G

A

A

D

D

I

C

L

T

V

V

T

N

T

V

C
|
C

S

P

F
|
F

C
|
C

I

H

L

L

N

Q

L

F

E

D

N
x
R

A

G

L

Q

E

I

K

E

T

I

K

K

N

E

Q

K

G

F

E

G

N

K

G

E

S

Y

V

N

E

F

R

P

V

V

M

L

D

H

L

L

T

S

E

Q

L

L

-

L

-

G

I

L

L

A

M

M

G

G

M

M

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G6), C9 (= C7), C41 (= C39), C42 (= C40), C78 (= C76), G80 (= G78), C81 (= C79), C153 (= C128), H154 (= H129), C193 (= C162), C194 (= C163), C231 (= C200), F233 (= F202), C234 (= C203)
binding 1-thioethanesulfonic acid: A44 (vs. gap), P45 (vs. gap), G46 (vs. gap), G80 (= G78), H154 (= H129), G197 (= G166), G198 (≠ S167)
binding Coenzyme B: R201 (≠ K170), F233 (= F202), R240 (≠ N209)

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
26% identity, 99% coverage: 3:238/239 of query aligns to 5:271/291 of 5odhB

query
sites
5odhB

Y

F

F

F

K

L

G
|
G

C
|
C

V
x
I

V

M

R

P

E

N

K

R

L

Y

P

A

G

G

V

V

A

E

Q

A

A

A

T

T

E

R

K

T

I

V

L

M

K

E

K

K

A

L

G

G

V

V

D

E

Y

L

T

V

L

D

L

M

G

T

N

G

E

A

S

S

C
|
C

-

P

-

A

-
x
P

-
x
G

-

V

-

F

G

G

S

S

F

F

L

D

M

Q

R

K

T

T

G

W

F

L

K

T

D

L

E

A

A

A

E

-

V

-

M

-

K

-

N

-

L

-

Q

R

D

N

I

L

L

C

A

I

A

A

N

E

E

E

K

M

-

G

-

V

T

D

I

I

L

V

V

T

S

V

C
|
C

S

N

G
|
G

C
|
C

Y

Y

N

G

T

S

L

L

K

F

N

E

D

A

Y

A

K

H

A

L

L

L

F

H

D

D

V

N

E

K

-

E

-

A

-

L

-

N

-

F

-

V

-

N

-

E

-

K

-

L

-

D

-

K

-

V

-

G

-

K

-

E

-

Y

-

K

-

G

-

N

L

V

D

K

V

V

I

R

H

H

T

F

S

A

E

E

L

L

V

I

K

Y

E

N

L

D

L

I

V

G

K

V

N

D

K

K

I

I

H

-

V

A

N

E

S

K

T

V

E

E

K

R

T

P

-

L

-

N

-

I

-

N

V

V

T

G

Y

V

H

H

D

Y

P
x
G

C
|
C

H
|
H

L

F

-

L

-

K

-

P

-

T

-

D

G

V

R

K

H

H

S

L

G

G

I

S

Y

A

E

E

E

R

P

P

-

V

-

M

R

L

D

D

A

E

I

I

K

-

A

V

S

E

A

A

D

T

L

G

V

A

E

K

M

S

K

V

R

P

N

Y

R

A

E

D

R

K

S

M

R

M

C
|
C

G

G

A

A

G

G

S

G

G

G

V

V

K

R

S

A

A

R

F

E

P

L

E

E

L

L

A

S

L

L

E

D

V

M

G

T

C

N

R

E

R

K

I

I

Q

E

D

N

A

M

E

I

E

K

V

A

G

G

A

A

D

D

I

C

L

T

V

V

T

N

T

V

C
|
C

S

P

F
|
F

C
|
C

I

H

L

L

N

Q

L

F

E

D

N

R

A

G

L

Q

E

I

K

E

T

I

K

K

N

E

Q

K

G

F

E

G

N

K

G

E

S

Y

V

N

E

F

R

P

V

V

M

L

D

H

L

L

T

S

E

Q

L

L

-

L

-

G

I

L

L

A

M

M

G

G

M

M

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G6), C9 (= C7), I10 (≠ V8), C41 (= C39), C42 (= C40), C78 (= C76), G80 (= G78), C81 (= C79), G152 (≠ P127), C153 (= C128), H154 (= H129), C193 (= C162), C194 (= C163), C231 (= C200), F233 (= F202), C234 (= C203)
binding 1-thioethanesulfonic acid: P45 (vs. gap), G46 (vs. gap), G80 (= G78), F233 (= F202)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
26% identity, 99% coverage: 3:238/239 of query aligns to 5:271/291 of 5odcB

query
sites
5odcB

Y

F

F

F

K

L

G
|
G

C
|
C

V
x
I

V

M

R

P

E

N

K

R

L

Y

P

A

G

G

V

V

A

E

Q

A

A

A

T

T

E

R

K

T

I

V

L

M

K

E

K

K

A

L

G

G

V

V

D

E

Y

L

T

V

L

D

L

M

G

T

N

G

E

A

S

S

C
|
C

-

P

-

A

-

P

-

G

-

V

-

F

G

G

S

S

F

F

L

D

M

Q

R

K

T

T

G

W

F

L

K

T

D

L

E

A

A

A

E

-

V

-

M

-

K

-

N

-

L

-

Q

R

D

N

I

L

L

C

A

I

A

A

N

E

E

E

K

M

-

G

-

V

T

D

I

I

L

V

V

T

S

V

C
|
C

S
x
N

G
|
G

C
|
C

Y

Y

N

G

T

S

L

L

K

F

N

E

D

A

Y

A

K

H

A

L

L

L

F

H

D

D

V

N

E

K

-

E

-

A

-

L

-

N

-

F

-

V

-

N

-

E

-

K

-

L

-

D

-

K

-

V

-

G

-

K

-

E

-

Y

-

K

-

G

-

N

L

V

D

K

V

V

I

R

H

H

T

F

S

A

E

E

L

L

V

I

K

Y

E

N

L

D

L

I

V

G

K

V

N

D

K

K

I

I

H

-

V

A

N

E

S

K

T

V

E

E

K

R

T

P

-

L

-

N

-

I

-

N

V

V

T

G

Y

V

H

H

D

Y

P
x
G

C
|
C

H

H

L

F

-

L

-

K

-

P

-

T

-

D

G

V

R

K

H

H

S

L

G

G

I

S

Y

A

E

E

E

R

P

P

-

V

-

M

R

L

D

D

A

E

I

I

K

-

A

V

S

E

A

A

D

T

L

G

V

A

E

K

M

S

K

V

R

P

N

Y

R

A

E

D

R

K

S

M

R

M

C
|
C

G

G

A

A

G
|
G

S

G

G

G

V

V

K

R

S

A

A

R

F

E

P

L

E

E

L

L

A

S

L

L

E

D

V

M

G

T

C

N

R

E

R

K

I

I

Q

E

D

N

A

M

E

I

E

K

V

A

G

G

A

A

D

D

I

C

L

T

V

V

T

N

T

V

C
|
C

S

P

F
|
F

C
|
C

I

H

L

L

N

Q

L

F

E

D

N

R

A

G

L

Q

E

I

K

E

T

I

K

K

N

E

Q

K

G

F

E

G

N

K

G

E

S

Y

V

N

E

F

R

P

V

V

M

L

D

H

L

L

T

S

E

Q

L

L

-

L

-

G

I

L

L

A

M

M

G

G

M

M

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G6), C9 (= C7), I10 (≠ V8), C41 (= C39), C42 (= C40), C78 (= C76), N79 (≠ S77), G80 (= G78), C81 (= C79), G152 (≠ P127), C153 (= C128), C193 (= C162), C194 (= C163), G197 (= G166), C231 (= C200), F233 (= F202), C234 (= C203)

Query Sequence

>WP_013644575.1 NCBI__GCF_000191585.1:WP_013644575.1
MLYFKGCVVREKLPGVAQATEKILKKAGVDYTLLGNESCCGSFLMRTGFKDEAEEVMKNN
LQDILAANEKTILVSCSGCYNTLKNDYKALFDVELDVIHTSELVKELLVKNKIHVNSTEK
TVTYHDPCHLGRHSGIYEEPRDAIKASADLVEMKRNRERSRCCGAGSGVKSAFPELALEV
GCRRIQDAEEVGADILVTTCSFCILNLENALEKTKNQGENGSVERVMDLTELILMGMEK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory