SitesBLAST
Comparing WP_013645675.1 NCBI__GCF_000191585.1:WP_013645675.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q4WR83 Acyl-CoA ligase sidI; Siderophore biosynthesis protein I; EC 6.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
39% identity, 99% coverage: 7:552/552 of query aligns to 38:587/590 of Q4WR83
Sites not aligning to the query:
- 6:14 PTS2-type peroxisomal targeting signal
8wevA Crystal structure of feruoyl-coa synthetase complexed with amp from amycolatopsis thermoflava
34% identity, 91% coverage: 34:537/552 of query aligns to 30:486/486 of 8wevA
Sites not aligning to the query:
P69451 Long-chain-fatty-acid--CoA ligase; Long-chain acyl-CoA synthetase; Acyl-CoA synthetase; EC 6.2.1.3 from Escherichia coli (strain K12) (see paper)
30% identity, 97% coverage: 6:538/552 of query aligns to 23:553/561 of P69451
- Y213 (= Y196) mutation to A: Loss of activity.
- T214 (= T197) mutation to A: 10% of wild-type activity.
- G216 (= G199) mutation to A: Decreases activity.
- T217 (= T200) mutation to A: Decreases activity.
- G219 (= G202) mutation to A: Decreases activity.
- K222 (= K205) mutation to A: Decreases activity.
- E361 (= E341) mutation to A: Loss of activity.
4gxqA Crystal structure of atp bound rpmatb-bxbclm chimera b1 (see paper)
30% identity, 91% coverage: 33:536/552 of query aligns to 28:499/506 of 4gxqA
- active site: T163 (= T197), N183 (= N217), H207 (= H241), T303 (= T340), E304 (= E341), I403 (= I443), N408 (= N448), A491 (≠ K528)
- binding adenosine-5'-triphosphate: T163 (= T197), S164 (= S198), G165 (= G199), T166 (= T200), T167 (= T201), H207 (= H241), S277 (≠ G313), A278 (≠ S314), P279 (= P315), E298 (≠ I335), M302 (≠ L339), T303 (= T340), D382 (= D422), R397 (= R437)
- binding carbonate ion: H207 (= H241), S277 (≠ G313), R299 (≠ A336), G301 (= G338)
P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
29% identity, 96% coverage: 6:537/552 of query aligns to 2:496/503 of P9WQ37
- R9 (≠ E13) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
- R17 (≠ S21) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
- K172 (= K205) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
- R195 (≠ T228) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
- R197 (≠ E230) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
- V209 (≠ C242) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
- A211 (≠ G244) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
- T214 (≠ L247) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
- R244 (≠ S278) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
- A302 (≠ G338) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
- W377 (= W417) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
- D382 (= D422) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
- R397 (= R437) mutation to A: Reduction of binding affinity for fatty acids.
- S404 (≠ R444) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
- G406 (= G446) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
- K487 (= K528) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.
Q9S725 4-coumarate--CoA ligase 2; 4CL 2; 4-coumarate--CoA ligase isoform 2; At4CL2; 4-coumaroyl-CoA synthase 2; Caffeate--CoA ligase; EC 6.2.1.12; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
29% identity, 91% coverage: 34:535/552 of query aligns to 64:547/556 of Q9S725
- K211 (= K205) mutation to S: Drastically reduces the activity.
- M293 (≠ Y283) mutation M->A,P: Affects the substrate specificity.
- K320 (≠ I310) mutation K->L,A: Affects the substrate specificity.
- E401 (= E389) mutation to Q: Slighlty reduces the substrate specificity.
- C403 (= C391) mutation to A: Significantly reduces the substrate specificity.
- R449 (= R437) mutation to Q: Drastically reduces the activity.
- K457 (≠ G445) mutation to S: Drastically reduces the activity.
- K540 (= K528) mutation to N: Abolishes the activity.
Q67W82 4-coumarate--CoA ligase 4; 4CL 4; Os4CL4; (E)-ferulate--CoA ligase; 4-coumaroyl-CoA synthase 4; Protein RESISTANCE TO ALUMINUM 1; EC 6.2.1.12; EC 6.2.1.34 from Oryza sativa subsp. japonica (Rice) (see paper)
30% identity, 95% coverage: 12:535/552 of query aligns to 39:542/559 of Q67W82
- G395 (= G388) mutation to R: In ral1; reduced lignin content and increased accumulation of 4-coumarate and ferulate in roots; confers increased tolerance to aluminum.
3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
29% identity, 96% coverage: 6:537/552 of query aligns to 5:496/502 of 3r44A
Q84P21 Peroxisomal OPC-8:0-CoA ligase 1; 4-coumarate--CoA ligase isoform 9; At4CL9; 4-coumarate--CoA ligase-like 5; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 93% coverage: 34:546/552 of query aligns to 58:546/546 of Q84P21