SitesBLAST

8a6tC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
49% identity, 90% coverage: 5:146/158 of query aligns to 7:148/151 of 8a6tC

query
sites
8a6tC

L

V

N

D

E

E

I

I

L

L

S

S

T

K

Y

L

E

A

G

N

T

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K

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S

G

E

A

L

L

I

I

P

A

L

I

L

L

Q

Q

D

H

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V

Q

Q

A

H

N

E

L

F

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G

Y

Y

L

L

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P

E

E

E

D

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I

K

F

D

Y

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I

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A

K

S

F

K

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T

G

G

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I

S

P

E

A

S

S

E

K

I

I

Y

Y

G

G

V

V

A

A

T

T

F

F

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Y

T

A

Q

Q

F

F

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H

F

L

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P

P

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R

G

G

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K

K

Y

H

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M

R

V

V

C
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C

K

L

G
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G

T

T

A
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A

C
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C

H

H

V

V

K

K

G

G

A

A

P

N

Q

K

I

I

I

L

E

A

G

E

I

F

E

E

R

K

H

Q

L

L

G

G

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I

K

K

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A

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G

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E

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T

T

T

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S

D

D

M

L

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K

Y

F

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T

L

L

E

E

S

R

V

V

G

G

C
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C

L
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L

G

G

C

A

C
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C

A

G

L

L

A

A

P

P

C

T

A

V

M

M

I

V

N

N

D

E

D

K

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T

E

Y

S

G

N

K

M

M

T

T

L

P

K

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K

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K

binding fe2/s2 (inorganic) cluster: C77 (= C75), G79 (= G77), A81 (= A79), C82 (= C80), C118 (= C116), L119 (= L117), C122 (= C120)

O52681 Bifurcating [FeFe] hydrogenase gamma subunit; Hydrogenase (NAD(+), ferredoxin) gamma subunit; Iron-hydrogenase gamma subunit; EC 1.12.1.4 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
45% identity, 96% coverage: 5:156/158 of query aligns to 8:159/161 of O52681

query
sites
O52681

L

V

N

E

E

E

I

I

L

L

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K

T

K

Y

Y

E

G

G

Y

T

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E

N

L

L

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I

P

K

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L

L

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L

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A

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Y

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Y

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G

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A

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T

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F

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Y

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Q

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F

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L

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P

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G

G

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K

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H

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M

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C

C

K
x
D

G
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G

T
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T

A
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A

C

C

H

H

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M

K

A

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G

A

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P

P

Q

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A

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I

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R

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M

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L

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G

C

C

L
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L

G
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G

C
x
A

C

C

A

A

L

L

A

A

P

P

C
x
V

A

M

M

V

I

I

N

N

D

G

D

E

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E

Y

S

G

N

N

M

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L

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A

D79 (≠ K76) mutation to P: Increase (30 mV) in the redox potential.
G80 (= G77) mutation to P: Large decrease (149 mV) in the redox potential. Causes a change to a [4Fe-4S] cluster protein.
T81 (= T78) mutation to P: Small increase (11 mV) in the redox potential.
A82 (= A79) mutation to P: Decrease (46 mV) in the redox potential.
L120 (= L117) mutation to P: Large decrease (65 mV) in the redox potential.
G121 (= G118) mutation to P: Decrease (22 mV) in the redox potential.
A122 (≠ C119) mutation to P: Large decrease (132 mV) in the redox potential.
V128 (≠ C125) mutation V->A,C,H,Y: Small increase (about 2-14 mV) in the redox potential.; mutation V->D,G,R,S,T,W: Increase (about 23-33 mV) in the redox potential.; mutation V->E,K,L,M: Decrease (about 37-50 mV) in the redox potential.; mutation V->F,I,P,Q: Small decrease (about 4-20 mV) in the redox potential.; mutation to N: Large increase (85 mV) in the redox potential.

7p5hC Tmhydabc- d2 map (see paper)
46% identity, 94% coverage: 5:153/158 of query aligns to 5:153/156 of 7p5hC

query
sites
7p5hC

L

V

N

E

E

E

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I

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L

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K

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Y

Y

E

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G

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N

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M

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G

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C

H

H

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G

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C

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L

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G

C
x
A

C
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C

A

A

L

L

A

A

P

P

C

V

A

M

M

V

I

I

N

N

D

G

D

E

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Y

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G

N

N

M

L

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T

L

A

K

D

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K

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F

L

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R

R

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I

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E

K

R

binding fe2/s2 (inorganic) cluster: C75 (= C75), G77 (= G77), A79 (= A79), C80 (= C80), C116 (= C116), L117 (= L117), A119 (≠ C119), C120 (= C120)

8oh5A Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
42% identity, 91% coverage: 5:148/158 of query aligns to 6:149/150 of 8oh5A

query
sites
8oh5A

L

L

N

E

E

P

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K

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E

A

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G

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K

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L

L

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Y

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C

L
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C
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C

A

S

L

L

A

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P

P

C

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A

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M

M

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N

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I

I

binding fe2/s2 (inorganic) cluster: C76 (= C75), C81 (= C80), C117 (= C116), L118 (= L117), G119 (= G118), C120 (= C119), C121 (= C120)

8a5eC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
39% identity, 82% coverage: 19:147/158 of query aligns to 27:155/156 of 8a5eC

query
sites
8a5eC

L

L

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C
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C

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Y

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A

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x
R

C
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C

L

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C

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C
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C

A

G

L

L

A

A

P

P

C

V

A

M

M

M

I

I

N

N

D

E

V

V

E

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M

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L

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D

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K

binding fe2/s2 (inorganic) cluster: C83 (= C75), G85 (= G77), A87 (= A79), C88 (= C80), R123 (≠ G115), C124 (= C116), C128 (= C120)

8eswV2 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
38% identity, 93% coverage: 3:149/158 of query aligns to 26:174/214 of 8eswV2

query
sites
8eswV2

K

K

T
x
R

L

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N

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E
x
A

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L

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S

T

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Y

Y

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E

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C

H

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D

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T

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C

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L

L

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G

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E

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C
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C

L
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L

G
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G

C

A

C
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C

A

V

L

N

A

A

P

P

C

M

A

V

M

A

I

I

N

N

D

D

V

Y

E

Y

S

E

N

D

M

L

T

T

L

S

K

K

D

D

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M

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Q

K

D

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I

F

L

R

N

R

D

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L

E

K

binding fe2/s2 (inorganic) cluster: C100 (= C75), C105 (= C80), C141 (= C116), L142 (= L117), G143 (= G118), C145 (= C120)
binding : R27 (≠ T4), A30 (≠ E7), E37 (= E13), R53 (≠ A28)

8b9zE Drosophila melanogaster complex i in the active state (dm1) (see paper)
38% identity, 93% coverage: 3:149/158 of query aligns to 26:174/214 of 8b9zE

query
sites
8b9zE

K

K

T

R

L

V

N

E

E

A

I

I

L

L

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S

T

I

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Y

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P

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F

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M

F

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M

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K

P

P

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T

G

G

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K

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H

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C
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C

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x
T

T
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T

A
x
P

C
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C

H

W

V

L

K

R

G

G

A

S

P

D

Q

D

I

I

I

L

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G

T

I

C

E

K

R

K

H

Q

L

L

G

G

I

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K

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G

G

E

D

V

T

T

T

F

K

D

D

M

R

E

K

Y

F

S

T

L

I

E

S

S

E

V

V

G

E

C
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C

L
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L

G
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G

C
x
A

C
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C

A

V

L

N

A

A

P

P

C

M

A

V

M

A

I

I

N

N

D

D

V

Y

E

Y

S

E

N

D

M

L

T

T

L

S

K

K

D

D

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M

K

Q

K

D

I

I

F

L

R

N

R

D

I

L

E

K

binding fe2/s2 (inorganic) cluster: C100 (= C75), T102 (≠ G77), T103 (= T78), P104 (≠ A79), C105 (= C80), C141 (= C116), L142 (= L117), G143 (= G118), A144 (≠ C119), C145 (= C120)

3i9v2 Crystal structure of the hydrophilic domain of respiratory complex i from thermus thermophilus, oxidized, 2 mol/asu (see paper)
37% identity, 80% coverage: 5:131/158 of query aligns to 9:137/178 of 3i9v2

query
sites
3i9v2

L

L

N

E

E

E

I

T

L

F

S

A

T

K

Y

Y

-

P

E

E

G

G

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A

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A

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F

Y

Y

T
x
S

Q

Y

F

Y

R

Q

F

F

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P

P

V

T

G

G

K

K

K

Y

H

H

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C
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C

K

A

G
x
T

T

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A
x
S

C
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C

H

K

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K

A

G

G

A

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L

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V

V

T

T

F

P

D

D

M

G

E

L

Y

F

S

S

L

V

E

Q

S

K

V
|
V

G
x
E

C
|
C

L
|
L

G
|
G

C
x
S

C
|
C

A

H

L

T

A

A

P

P

C

V

A

I

M

Q

I

V

N

N

D

D

D

E

binding fe2/s2 (inorganic) cluster: C81 (= C75), T83 (≠ G77), S85 (≠ A79), C86 (= C80), C122 (= C116), L123 (= L117), G124 (= G118), S125 (≠ C119), C126 (= C120)
binding manganese (ii) ion: S66 (≠ T60), V120 (= V114), E121 (≠ G115)

3iam2 Crystal structure of the hydrophilic domain of respiratory complex i from thermus thermophilus, reduced, 2 mol/asu, with bound nadh (see paper)
37% identity, 80% coverage: 5:131/158 of query aligns to 10:138/179 of 3iam2

query
sites
3iam2

L

L

N

E

E

E

I

T

L

F

S

A

T

K

Y

Y

-

P

E

E

G

G

T

R

K

R

S

A

E

A

L

I

I

M

P

P

L

L

Q

R

D

R

V

V

Q

Q

A

Q

N

E

L

E

G

G

Y

W

L

I

S

R

E

P

E

E

S

R

I

I

K

E

D

E

V

I

S

A

K

R

F

L

T

V

G

G

V

T

S

T

E

P

S

T

E

E

I

V

Y

M

G

G

V

V

A

A

T

S

F

F

Y

Y

T

S

Q

Y

F

Y

R

Q

F

F

T

V

P

P

V

T

G

G

K

K

K

Y

H

H

I

L

M

Q

V

V

C
|
C

K

A

G

T

T

L

A

S

C
|
C

H

K

V

L

K

A

G

G

A

A

P

E

Q

E

I

L

I

W

E

D

G

Y

I

L

E

T

R

E

H

T

L

L

G

G

I

I

K

G

E

P

G

G

E

E

V

V

T

T

F

P

D

D

M

G

E

L

Y

F

S

S

L

V

E

Q

S

K

V

V

G

E

C
|
C

L

L

G

G

C

S

C
|
C

A

H

L

T

A

A

P

P

C

V

A

I

M

Q

I

V

N

N

D

D

D

E

binding fe2/s2 (inorganic) cluster: C82 (= C75), C87 (= C80), C123 (= C116), C127 (= C120)

Q56221 NADH-quinone oxidoreductase subunit 2; NADH dehydrogenase I chain 2; NDH-1 subunit 2; EC 7.1.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
37% identity, 80% coverage: 5:131/158 of query aligns to 11:139/181 of Q56221

query
sites
Q56221

L

L

N

E

E

E

I

T

L

F

S

A

T

K

Y

Y

-

P

E

E

G

G

T

R

K

R

S

A

E

A

L

I

I

M

P

P

L

L

Q

R

D

R

V

V

Q

Q

A

Q

N

E

L

E

G

G

Y

W

L

I

S

R

E

P

E

E

S

R

I

I

K

E

D

E

V

I

S

A

K

R

F

L

T

V

G

G

V

T

S

T

E

P

S

T

E

E

I

V

Y

M

G

G

V

V

A

A

T

S

F

F

Y

Y

T

S

Q

Y

F

Y

R

Q

F

F

T

V

P

P

V

T

G

G

K

K

K

Y

H

H

I

L

M

Q

V

V

C
|
C

K

A

G

T

T

L

A
x
S

C
|
C

H

K

V

L

K

A

G

G

A

A

P

E

Q

E

I

L

I

W

E

D

G

Y

I

L

E

T

R

E

H

T

L

L

G

G

I

I

K

G

E

P

G

G

E

E

V

V

T

T

F

P

D

D

M

G

E

L

Y

F

S

S

L

V

E

Q

S

K

V

V

G

E

C
|
C

L

L

G

G

C

S

C
|
C

A

H

L

T

A

A

P

P

C

V

A

I

M

Q

I

V

N

N

D

D

D

E

C83 (= C75) binding [2Fe-2S] cluster
S87 (≠ A79) binding [2Fe-2S] cluster
C88 (= C80) binding [2Fe-2S] cluster
C124 (= C116) binding [2Fe-2S] cluster
C128 (= C120) binding [2Fe-2S] cluster

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
144 modified: Disulfide link with 172
172 modified: Disulfide link with 144

5gupE structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
38% identity, 93% coverage: 3:149/158 of query aligns to 39:187/197 of 5gupE

query
sites
5gupE

K

K

T

R

L

I

N

E

E

A

I

I

L

V

S

K

T

N

Y

Y

-

P

E

E

G

G

T

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

L

V

L

L

Q

D

D

L

V

A

Q

Q

A

R

N

Q

L

N

G

G

Y

W

L

L

S

P

E

I

E

S

S

A

I

M

K

N

D

K

V

V

S

A

K

E

F

I

T

L

G

Q

V

V

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

K

T

G
x
T

T

T

A
x
P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

Q

S

I

I

I

L

E

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

F

P

D

D

M

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L
|
L

G
|
G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C
x
M

A

V

M

Q

I

I

N

N

D

D

D

N

V

Y

E

Y

S

E

N

D

M

L

T

T

L

P

K

K

D

D

V

I

K

E

I

I

F

I

R

D

R

E

I

L

E

K

binding fe2/s2 (inorganic) cluster: C113 (= C75), T115 (≠ G77), P117 (≠ A79), C118 (= C80), C154 (= C116), L155 (= L117), G156 (= G118), A157 (≠ C119), C158 (= C120), M163 (≠ C125)

7dgq9 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (see paper)
38% identity, 93% coverage: 3:149/158 of query aligns to 22:170/207 of 7dgq9

query
sites
7dgq9

K

K

T
x
R

L

I

N

E

E

A

I

I

L

V

S
x
K

T

N

Y

Y

-
x
P

E

E

G

G

T
x
H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

L

V

L

L

Q

D

D

L

V

A

Q

Q

A
x
R

N

Q

L

N

G

G

Y

W

L

L

S

P

E

I

E

S

S

A

I

M

K

N

D

K

V

V

S

A

K

E

F

I

T

L

G

Q

V

V

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

K

T

G
x
T

T

T

A

P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

Q

S

I

I

I

L

E

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

F

P

D

D

M

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L
|
L

G

G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

I

I

N

N

D

D

D

N

V

Y

E

Y

S

E

N

D

M

L

T

T

L

P

K

K

D

D

V

I

K

E

I

I

F

I

R

D

R

E

I

L

E

K

binding fe2/s2 (inorganic) cluster: C96 (= C75), T98 (≠ G77), C101 (= C80), C137 (= C116), L138 (= L117), A140 (≠ C119), C141 (= C120)
binding : R23 (≠ T4), K29 (≠ S10), P32 (vs. gap), H35 (≠ T15), R49 (≠ A28)

Sites not aligning to the query:

binding : 20

8e9gE Mycobacterial respiratory complex i with both quinone positions modelled (see paper)
37% identity, 87% coverage: 7:144/158 of query aligns to 34:171/233 of 8e9gE

query
sites
8e9gE

E

Q

I

I

L

I

S

A

T

R

Y

Y

E

P

G

D

T

A

K

R

S

S

E

A

L

L

I

L

P

P

L

L

Q

H

D

L

V

V

Q

Q

A

A

N

Q

L

D

G

G

Y

Y

L

L

S

T

E

P

E

A

S

G

I

I

K

G

D

F

V

C

S

A

K

A

F

Q

T

L

G

G

V

L

S

T

E

E

S

A

E

E

I

V

Y

T

G

A

V

V

A

A

T

T

F

F

Y

Y

T

S

Q

M

F

Y

R

R

F

R

T

T

P

P

V

T

G

G

K

D

K

Y

H

L

I

V

M

G

V

V

C
|
C

K

T

G

N

T

T

A

L

C
|
C

H

A

V

I

K

M

G

G

A

G

P

D

Q

A

I

I

I

L

E

E

G

A

I

L

E

E

R

D

H

H

L

L

G

G

I

V

K

H

E

P

G

G

E

Q

V

T

T

T

F

P

D

D

M

G

E

R

Y

V

S

T

L

L

E

E

S

H

V

V

G

E

C
|
C

L
x
N

G
x
A

C
x
A

C
|
C

A

D

L

Y

A

A

P

P

C
x
V

A

V

M

M

I

V

N

N

D

W

D

E

V

F

E

Y

S

D

N

N

M

Q

T

T

L

P

K

S

D

S

V

A

K

R

K

D

I

L

binding fe2/s2 (inorganic) cluster: C102 (= C75), C107 (= C80), C143 (= C116), N144 (≠ L117), A145 (≠ G118), A146 (≠ C119), C147 (= C120), V152 (≠ C125)

P19404 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NDUFV2; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Homo sapiens (Human) (see 2 papers)
38% identity, 93% coverage: 3:149/158 of query aligns to 61:209/249 of P19404

query
sites
P19404

K

K

T

R

L

I

N

E

E

A

I

I

L

V

S

K

T

N

Y

Y

-

P

E

E

G

G

T

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

L

V

L

L

Q

D

D

L

V

A

Q

Q

A

R

N

Q

L

N

G

G

Y

W

L

L

S

P

E

I

E

S

S

A

I

M

K

N

D

K

V

V

S

A

K

E

F

V

T

L

G

Q

V

V

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C

C

K

T

G

T

T

T

A

P

C

C

H

M

V

L

K

R

G

N

A

S

P

D

Q

S

I

I

I

L

E

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

F

P

D

D

M

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C

C

L

L

G

G

C

A

C

C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

I

I

N

N

D

D

D

N

V

Y

E
x
Y

S

E

N

D

M

L

T

T

L

A

K

K

D

D

V

I

K

E

I

I

F

I

R

D

R

E

I

L

E

K

Y193 (≠ E133) modified: Phosphotyrosine; by SRC

Sites not aligning to the query:

29 V → A: in dbSNP:rs906807

P04394 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase subunit II; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Bos taurus (Bovine) (see 2 papers)
38% identity, 93% coverage: 3:149/158 of query aligns to 61:209/249 of P04394

query
sites
P04394

K

K

T

R

L

I

N

E

E

A

I

I

L

V

S

K

T

N

Y

Y

-

P

E

E

G

G

T

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

L

V

L

L

Q

D

D

L

V

A

Q

Q

A

R

N

Q

L

N

G

G

Y

W

L

L

S

P

E

I

E

S

S

A

I

M

K

N

D

K

V

V

S

A

K

E

F

I

T

L

G

Q

V

V

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

K

T

G

T

T

T

A

P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

Q

S

I

I

I

L

E

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

F

P

D

D

M

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L

L

G

G

C

A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

I

I

N

N

D

D

D

N

V

Y

E

Y

S

E

N

D

M

L

T

T

L

P

K

K

D

D

V

I

K

E

I

I

F

I

R

D

R

E

I

L

E

K

C135 (= C75) binding [2Fe-2S] cluster
C140 (= C80) binding [2Fe-2S] cluster
C176 (= C116) binding [2Fe-2S] cluster
C180 (= C120) binding [2Fe-2S] cluster

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

7v2cO Active state complex i from q10 dataset (see paper)
38% identity, 93% coverage: 3:149/158 of query aligns to 29:177/217 of 7v2cO

query
sites
7v2cO

K

K

T

R

L

I

N

E

E

A

I

I

L

V

S

K

T

N

Y

Y

-

P

E

E

G

G

T

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

L

V

L

L

Q

D

D

L

V

A

Q

Q

A

R

N

Q

L

N

G

G

Y

W

L

L

S

P

E

I

E

S

S

A

I

M

K

N

D

K

V

V

S

A

K

E

F

I

T

L

G

Q

V

V

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

K

T

G

T

T

T

A
x
P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

Q

S

I

I

I

L

E

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

F

P

D

D

M

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L
|
L

G
|
G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

I

I

N

N

D

D

D

N

V

Y

E

Y

S

E

N

D

M

L

T

T

L

P

K

K

D

D

V

I

K

E

I

I

F

I

R

D

R

E

I

L

E

K

binding fe2/s2 (inorganic) cluster: C103 (= C75), P107 (≠ A79), C108 (= C80), C144 (= C116), L145 (= L117), G146 (= G118), A147 (≠ C119), C148 (= C120)

Q9D6J6 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Mus musculus (Mouse) (see paper)
38% identity, 91% coverage: 3:145/158 of query aligns to 60:204/248 of Q9D6J6

query
sites
Q9D6J6

K

K

T

R

L

I

N

E

E

A

I

I

L

V

S

K

T

N

Y

Y

-

P

E

E

G

G

T

H

K

Q

S

A

E

A

-

A

L

V

I

L

P

P

L

V

L

L

Q

D

D

L

V

A

Q

Q

A

R

N

Q

L

N

G

G

Y

W

L

L

S

P

E

I

E

S

S

A

I

M

K

N

D

K

V

V

S

A

K

E

F

V

T

L

G

Q

V

V

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

K

T

G

T

T

T

A

P

C
|
C

H

M

V

L

K

R

G

D

A

S

P

D

Q

S

I

I

I

L

E

E

G

T

I

L

E

Q

R

R

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

F

P

D

D

M

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L

L

G

G

C

A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

I

I

N

N

D

D

D

N

V

Y

E

Y

S

E

N

D

M

L

T

T

L

P

K

K

D

D

V

I

K

E

I

I

F

I

C134 (= C75) binding [2Fe-2S] cluster
C139 (= C80) binding [2Fe-2S] cluster
C175 (= C116) binding [2Fe-2S] cluster
C179 (= C120) binding [2Fe-2S] cluster

6tg9G Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
34% identity, 91% coverage: 2:144/158 of query aligns to 3:143/148 of 6tg9G

query
sites
6tg9G

T

T

K

A

T

R

L

L

N

R

E

A

I

I

L

L

S

A

T

A

Y

H

E

R

G

G

T

R

K

E

S

G

E

A

L

L

I

L

P

P

L

I

L

L

Q

H

D

D

V

V

Q

Q

A

A

N

A

L

F

G

G

Y

F

L

I

S

P

E

E

E

D

S

A

I

Y

K

A

D

P

V

I

S

A

K

A

F

D

T

L

G

G

V

L

S

T

E

R

S

A

E

E

I

V

Y

A

G

G

V

V

A

V

T

G

F

F

Y

Y

T

H

Q

D

F

F

R

R

F

K

T

A

P

P

V

A

G

G

K

R

K

H

H

V

I

I

M

K

V

L

C
|
C

K

R

G
x
A

T

E

A

A

C
|
C

H

Q

V

A

K

M

G

G

A

M

P

D

Q

A

I

V

I

Q

E

A

G

R

I

L

E

E

R

S

H

A

L

L

G

G

I

L

K

R

E

L

G

G

E

D

V

S

T

S

F

-

D

-

M

E

E

A

Y

V

S

T

L

L

E

E

S

A

V

V

G

Y

C
|
C

L
|
L

G

G

C
x
L

C
|
C

A

A

L

C

A

A

P

P

C

A

A

A

M

M

I

V

N

D

D

D

D

R

V

L

E

V

S

G

N

R

M

L

T

D

L

A

K

A

D

A

V

V

K

A

K

G

I

I

binding fe2/s2 (inorganic) cluster: C76 (= C75), A78 (≠ G77), C81 (= C80), C115 (= C116), L116 (= L117), L118 (≠ C119), C119 (= C120)

7b0nE 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
36% identity, 91% coverage: 3:145/158 of query aligns to 26:170/216 of 7b0nE

query
sites
7b0nE

K

K

T

R

L

A

N

E

E

E

I

V

L

I

S

A

T

K

Y

Y

-

P

E

Q

G

Y

T

K

K

K

S

A

E

A

L

V

I

M

P

P

L

L

Q

D

D

I

V

G

Q

Q

A

R

N

Q

L

L

G

G

Y

Y

L

T

S

S

E

I

E

S

S

V

I

M

K

N

D

Y

V

V

S

A

K

K

F

L

T

L

G

E

V

M

S

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

T

T

Q

M

F

Y

R

N

F

R

T

T

P

P

V

M

G

G

K

R

K

Y

H

H

I

L

M

Q

V

I

C
|
C

K

T

G
x
T

T

T

A
x
P

C
|
C

H

Q

V

L

K

C

G

G

A

S

P

D

Q

G

I

I

I

M

E

E

G

A

I

V

E

Q

R

N

H

T

L

L

G

N

I

I

K

K

E

P

G

G

E

E

V

T

T

T

F

K

D

D

M

N

E

L

Y

F

S

T

L

L

E

S

S

E

V

V

G

E

C
|
C

L
|
L

G

G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

M

M

A

I

I

N

N

D

D

V

Y

E

Y

S

E

N

D

M

L

T

T

L

P

K

E

D

G

V

T

K

V

K

K

I

L

F

L

binding fe2/s2 (inorganic) cluster: C100 (= C75), T102 (≠ G77), P104 (≠ A79), C105 (= C80), C141 (= C116), L142 (= L117), A144 (≠ C119), C145 (= C120)

Query Sequence

>WP_013646083.1 NCBI__GCF_000191585.1:WP_013646083.1
MTKTLNEILSTYEGTKSELIPLLQDVQANLGYLSEESIKDVSKFTGVSESEIYGVATFYT
QFRFTPVGKKHIMVCKGTACHVKGAPQIIEGIERHLGIKEGEVTFDMEYSLESVGCLGCC
ALAPCAMINDDVESNMTLKDVKKIFRRIERQSKSDGEN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory