SitesBLAST

W22 (≠ A16) mutation to A: Decreased heat stability.
E26 (= E20) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ K24) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ E28) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (= Y218) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (≠ K231) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E268) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
51% identity, 98% coverage: 2:300/305 of query aligns to 12:309/316 of Q81M99

query
sites
Q81M99

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STRT 57:60 (= STRT 51:54) binding carbamoyl phosphate
Q84 (= Q78) binding carbamoyl phosphate
R108 (= R102) binding carbamoyl phosphate
HPCQ 135:138 (= HPCQ 129:132) binding carbamoyl phosphate
N166 (= N160) binding L-ornithine
D230 (= D220) binding L-ornithine
SM 234:235 (= SM 224:225) binding L-ornithine
CL 269:270 (= CL 260:261) binding carbamoyl phosphate
R297 (= R288) binding carbamoyl phosphate

4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
51% identity, 98% coverage: 2:300/305 of query aligns to 8:305/307 of 4nf2A

query
sites
4nf2A

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active site: R55 (= R53), T56 (= T54), R83 (= R81), R104 (= R102), H131 (= H129), Q134 (= Q132), D226 (= D220), C265 (= C260), R293 (= R288)
binding phosphoric acid mono(formamide)ester: S53 (= S51), T54 (= T52), R55 (= R53), T56 (= T54), R104 (= R102), H131 (= H129), Q134 (= Q132), C265 (= C260), L266 (= L261), R293 (= R288)
binding norvaline: L126 (= L124), N162 (= N160), D226 (= D220), S230 (= S224), M231 (= M225)

8qeuA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with ornithine (see paper)
47% identity, 99% coverage: 1:301/305 of query aligns to 1:303/304 of 8qeuA

query
sites
8qeuA

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G

binding L-ornithine: L125 (= L124), N161 (= N160), D222 (= D220), S226 (= S224), M227 (= M225), L263 (= L261)

7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:305/308 of 7nouA

query
sites
7nouA

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K

W

L

L

I

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D220), C265 (= C260), R293 (= R288)
binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (= I46), T52 (= T52), R53 (= R53), R53 (= R53), F56 (≠ V56), F56 (≠ V56), L79 (= L79), D82 (≠ G82), E83 (= E83), V91 (= V91), Y95 (= Y95), L266 (= L261), R293 (= R288)

7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:305/308 of 7nosA

query
sites
7nosA

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I
|
I

F

F

E

D

L

K

P

N

S

S

T

T

R

R

T

T

R

R

V

F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

D

W

S

N

G

E

S

L

T

Q

Q

L

L

G

G

R

R

G
x
D

E
|
E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L
|
L

S

S

R

R

Y

Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

S

M

M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D220), C265 (= C260), R293 (= R288)
binding 4-bromanyl-6-(trifluoromethyl)-1~{H}-benzimidazole: I46 (= I46), D82 (≠ G82), E83 (= E83), V91 (= V91), L92 (= L92)

7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:305/308 of 7norA

query
sites
7norA

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I

I

F

F

E

D

L

K

P

N

S

S

T
|
T

R

R

T

T

R

R

V
x
F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

D

W

S

N

G

E

S

L
x
T

Q

Q

L
|
L

G

G

R

R

G

D

E

E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

Y

Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

S

M

M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D220), C265 (= C260), R293 (= R288)
binding 2-fluoro-4-hydroxybenzonitrile: T52 (= T52), F56 (≠ V56), T77 (≠ L77), L79 (= L79)

7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:305/308 of 7nnyA

query
sites
7nnyA

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I

I

F

F

E

D

L

K

P

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

D

W

S

N

G

E

S

L

T

Q

Q

L

L

G

G

R

R

G

D

E
|
E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

Y
|
Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

S

M

M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D220), C265 (= C260), R293 (= R288)
binding 1-naphthol: T52 (= T52), R53 (= R53), F56 (≠ V56), E83 (= E83), V91 (= V91), Y95 (= Y95)

7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:305/308 of 7nnwA

query
sites
7nnwA

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I
|
I

F

F

E

D

L

K

P

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

D

W

S

N

G

E

S

L

T

Q

Q

L
|
L

G

G

R

R

G

D

E

E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V

V

L
|
L

S

S

R

R

Y
|
Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

S

M

M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D220), C265 (= C260), R293 (= R288)
binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (= I46), T52 (= T52), R53 (= R53), F56 (≠ V56), L79 (= L79), L92 (= L92), Y95 (= Y95)

7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:305/308 of 7nnvA

query
sites
7nnvA

M

I

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F

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D

D

D

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L

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A

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A

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E

I

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L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

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T

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D

R

Y

P

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N

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-

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A

M

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T

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F

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A

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Y

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R

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L

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A

F

M

A

A

A

S

Y

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A

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P

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I

N

N

G

A

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|
H

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A

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D

L

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A

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A

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G

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L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

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V

V

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A

T

A

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P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

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A

A

A

V

A

K

A

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G

A

A

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D

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I

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|
D

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W

V

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S

M

M

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G

Q

Q

E

E

S

N

E

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G

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D

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K

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F

F

G

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P

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Q

Q

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N

N

K

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L

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A

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A

A

K

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D

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A

I

I

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M

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H

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C
|
C

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|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

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D

E

A

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V

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M

E

D

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G

A

P

S

A

S

S

V

A

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W

D

D

Q

E

A

A

E

E

N

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|
R

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L

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active site: R102 (= R102), H129 (= H129), Q132 (= Q132), D225 (= D220), C265 (= C260), R293 (= R288)
binding phosphoric acid mono(formamide)ester: S51 (= S51), T52 (= T52), R53 (= R53), T54 (= T54), R102 (= R102), H129 (= H129), C265 (= C260), L266 (= L261), R293 (= R288)

2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
45% identity, 98% coverage: 1:300/305 of query aligns to 2:304/307 of 2i6uA

query
sites
2i6uA

M

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A

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E

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-

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-

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-

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-

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A

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A

A

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R

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L

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-

A

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|
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A

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H

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L

L

I

L

L

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A

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E

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E

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-

A

-

E

K

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A

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E

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T

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G

A

A

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S

L

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T

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A

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D

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A

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A

A

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A

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|
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W

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|
S

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|
M

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G

Q

Q

E

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E

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G

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L

K

D

R

R

L

V

K

K

D

P

F

F

G

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P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

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A

I

I

V

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

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Q

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K

A

A

I

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L

L

L

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L

I

L

active site: R52 (= R53), T53 (= T54), R80 (= R81), R101 (= R102), H128 (= H129), Q131 (= Q132), D224 (= D220), C264 (= C260), R292 (= R288)
binding phosphoric acid mono(formamide)ester: S50 (= S51), T51 (= T52), R52 (= R53), T53 (= T54), R101 (= R102), C264 (= C260), L265 (= L261), R292 (= R288)
binding norvaline: L123 (= L124), N160 (= N160), D224 (= D220), S228 (= S224), M229 (= M225)

P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 98% coverage: 1:300/305 of query aligns to 2:304/307 of P9WIT9

query
sites
P9WIT9

M

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-

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F

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F

M

A

A

A

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Y

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I

N

N

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A

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K

R

V

L

A

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W

Y

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F

G

G

D

D

G

G

-

A

N

N

N
|
N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

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A

A

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I

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A

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S

E

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G

Y

F

E

L

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P

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D

E

P

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S

I

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I

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-

A

-

E

K

R

A

A

K

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E

D

L

T

K

G

A

A

N

S

L

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K

T

F

V

E

T

S

A

N

D

P

A

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H

K

A

A

A

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A

K

A

D

G

A

A

D

D

V

V

I

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Y

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D
|
D

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T

W

W

V

T

S
|
S

M
|
M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

STRT 50:53 (= STRT 51:54) binding carbamoyl phosphate
Q77 (= Q78) binding carbamoyl phosphate
R101 (= R102) binding carbamoyl phosphate
HPCQ 128:131 (= HPCQ 129:132) binding carbamoyl phosphate
N160 (= N160) binding L-ornithine
D224 (= D220) binding L-ornithine
SM 228:229 (= SM 224:225) binding L-ornithine
CL 264:265 (= CL 260:261) binding carbamoyl phosphate
R292 (= R288) binding carbamoyl phosphate

8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
45% identity, 99% coverage: 1:301/305 of query aligns to 1:296/297 of 8qevA

query
sites
8qevA

M

L

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K

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A

A

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K

K

A

A

E

L

R

L

Y

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G

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E

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N

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Y

L

L

-

P

-

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N

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A

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I

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F

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x
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|
T

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F

F

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L

L

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G

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H

A

A

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Y

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N

N

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G

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E

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E

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F

N

A

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I

E

L

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D

F

L

A

A

A

N

Y

Y

S

S

V

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P

P

V

V

I

V

N

N

G

G

L

L

S

T

N

D

L

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E

N

H

H

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P

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Q

I

I

I

M

A

A

D

D

L

A

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T

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E

E

D

G

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T

K

K

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V

A

V

W

Y

V

V

G

G

D

D

G

G

N

N

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M

C

V

N

H

S

S

L

W

I

L

L

E

A

L

T

A

A

S

L

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A

I

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P

M

F

E

H

I

F

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V

V

C

S

A

T

C

P

P

S

K

G

G

Y

Y

E

E

P

P

D

D

E

K

S

E

I

R

I

V

K

S

K

K

A

A

K

K

E

Q

L

A

K

G

-

L

A

S

N

K

L

I

K

E

F

I

E

T

S

N

N

D

P

P

V

K

K

E

A

A

V

V

K

I

D

G

A

A

D

D

V

V

I

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Y

Y

T

S

D

D

V

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W

W

V

A

S

A

M

-

G

-

Q

-

E

-

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-

E

-

K

-

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E

K

A

R

R

L

R

K

K

D

A

F

F

G

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G

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F

Q

Q

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V

N

D

K

E

K

A

L

L

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M

D

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V

L

A

A

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G

E

Q

D

K

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A

I

Y

V

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

H

E

R

R

G

G

E

V

E

E

I

V

T

T

D

N

E

G

V

V

I

V

E

E

G

A

A

P

S

Y

S

S

V

I

I

V

F

F

D

P

Q

Q

A

A

E

E

N

N

R
|
R

L

M

H

H

A

A

Q

Q

K

N

A

A

I

I

L

M

L

L

K

H

L

L

I

L

G

G

binding phosphoric acid mono(formamide)ester: S52 (= S51), M53 (≠ T52), R54 (= R53), T55 (= T54), R103 (= R102), C255 (= C260), L256 (= L261), R283 (= R288)

7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
45% identity, 98% coverage: 1:300/305 of query aligns to 3:302/305 of 7np0A

query
sites
7np0A

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I

I

F

F

E

D

L

K

P

N

S

S

T

T

R

R

T

T

R

R

V

F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

-

W

-

N

-

E

D

L

S

Q

G

L

L

G

G

R

R

G

D

E
|
E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

Y
|
Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

S

M

M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R99 (= R102), H126 (= H129), Q129 (= Q132), D222 (= D220), C262 (= C260), R290 (= R288)
binding p-nitrophenylboronic acid: E80 (= E83), V88 (= V91), Y92 (= Y95)

7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
44% identity, 98% coverage: 1:300/305 of query aligns to 3:299/302 of 7novA

query
sites
7novA

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I

I

F

F

E

D

L

K

P

N

S

S

T

T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

D

W

-

N

-

E

-

L

-

Q

-

L

-

G

S

R

R

G

D

E
|
E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

Y
|
Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

S

M

M

G

G

Q

Q

E

E

S

N

E

D

K

G

R

L

K

D

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A
|
A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R96 (= R102), H123 (= H129), Q126 (= Q132), D219 (= D220), C259 (= C260), R287 (= R288)
binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R53), F56 (≠ V56), E77 (= E83), V85 (= V91), Y89 (= Y95), L260 (= L261), A284 (= A285), R287 (= R288)

P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
42% identity, 98% coverage: 2:300/305 of query aligns to 40:342/354 of P00481

query
sites
P00481

K

R

H

D

L

L

S

T

I

L

S

K

D

N

L

F

T

T

A

G

E

E

L

I

A

Q

E

Y

I

M

L

L

E

W

L

L

A

S

E

A

K

D

L

L

K

K

A

F

E

R

-

I

R

K

Y

Q

K

K

G

G

V

E

V

Y

T

L

D

P

Y

L

L

L

K

Q

N

G

K

K

S

S

L

L

A

G

M

M

I

I

F

F

E

E

L

K

P

R

S

S

T

T

R
|
R

T

T

R

R

V

L

S

S

F

T

E

E

V

T

A

G

M

F

T

A

D

L

L

L

G

G

H

H

A

P

L

S

Y

F

L

L

G

T

W

T

N

Q

E

D

L

I

Q

H

L

L

G

G

R

V

G

N

E

E

P

S

I

L

K

T

D

D

T

T

A

A

R

R

V

V

L

L

S

S

R

S

Y

M

V

T

H

D

A

A

I

V

M

L

M

A

R

R

V

V

R

Y

N

K

H

Q

S

S

T

D

L

L

E

D

E

I

F

L

A

A

A

K

Y

E

S

A

S

T

V

I

P

P

V

I

I

V

N

N

G

G

L

L

S

S

N

D

L

L

E

Y

H

H

P

P

C

I

Q

Q

I

I

I

L

A

A

D

D

L

Y

L

L

T

T

I

L

K

Q

E

E

Y

H

K

Y

G

G

S

S

L

L

E

K

D

G

V

L

K

T

V

L

A

S

W

W

V

I

G

G

D

D

G

G

N

N

V

I

C

L

N

H

S

S

L

I

I

M

L

M

A

S

T

A

A

A

L

K

A

F

G

G

M

M

E

H

I

L

V

Q

V

A

S

A

T

T

P

P

S

K

G

G

Y

Y

E

E

P

P

D

D

E

P

S

N

I

I

I

V

K

K

-

L

-

A

-

E

-

Q

K

Y

A

A

K

K

E

E

L

N

K

G

A

T

N

R

L

L

K

S

F

M

E

T

S

N

N

D

P

P

V

L

K

E

A

A

V

A

K

R

D

G

A

G

D

N

V

V

I

L

Y

I

T

T

D

D

V

T

W

W

V

I

S

S

M

M

G

G

Q

Q

E

E

S

D

E

E

K

K

R

K

K

K

R

R

L

L

K

Q

D

A

F

F

G

Q

P

G

Y

Y

Q

Q

V

V

N

T

K

M

K

K

L

T

V

A

D

K

V

V

A

A

K

A

E

S

D

D

V

W

I

T

V

F

M

L

H

H

C
|
C

L

L

P

P

A

-

H

R

R

K

G

P

E

E

I

V

T

D

D

D

E

E

V

V

I

F

E

Y

G

S

A

P

S

R

S

S

V

L

I

V

F

F

D

P

Q

E

A

A

E

E

N

N

R

R

L

K

H

W

A

T

Q

I

K

M

A

A

I

V

L

M

L

V

K

S

L

L

I

L

R92 (= R53) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
C303 (= C260) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
44% identity, 98% coverage: 1:300/305 of query aligns to 2:294/297 of 7nnzB

query
sites
7nnzB

M

I

K

R

H

H

L

F

L

L

S

R

I

D

S

D

D

D

L

L

T

S

A

P

E

A

E

E

L

Q

A

A

E

E

I

V

L

L

E

E

L

L

A

A

E

A

K

E

L

L

K

K

A

K

E

D

R

-

Y

-

K

-

G

P

V

V

V

S

T

R

D

R

Y

P

L

L

K

Q

N

G

-

P

K

R

S

G

L

V

A

A

M

V

I

I

F

F

E

D

L

K

P

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

V

L

A

G

M

I

T

A

D

Q

L

L

G

G

H

H

A

A

L

V

Y

V

L

V

G

D

W

S

N

G

E

S

L

T

Q

Q

L

L

G

G

R

R

G

D

E

E

P

T

I

L

K

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

Y

Y

V

V

H

D

A

A

I

I

M

V

M

W

R
|
R

V

T

R

F

N

G

H

Q

S

E

T

R

L

L

E

D

E

A

F

M

A

A

A

S

Y

V

S

A

S

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

S

S

N

D

L

E

E

F

H
|
H

P

P

C

C

Q
|
Q

I

V

I

L

A

A

D

D

L

L

L

Q

T

T

I

I

K

A

E

E

Y

R

K

K

G

G

S

A

L

L

E

R

D

G

V

L

K

R

V

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

C

A

N

H

S

S

L

L

I

L

L

L

A

G

T

G

A

V

L

T

A

A

G

G

M

I

E

H

I

V

V

T

V

V

S

A

T

A

P

P

S

E

G

G

Y

F

E

L

P

P

D

D

E

P

S

S

I

V

I

R

-

A

-

E

K

R

A

A

K

Q

E

D

L

T

K

G

A

A

N

S

L

V

K

T

F

V

E

T

S

A

N

D

P

A

V

H

K

A

A

A

V

A

K

A

D

G

A

A

D

D

V

V

I

L

Y

V

T

T

D
|
D

V

T

W

W

V

T

S

-

M

-

G

-

Q

-

E

-

S

-

E

-

K

-

R

-

K

-

R

R

L

V

K

K

D

P

F

F

G

R

P

P

Y

F

Q

Q

V

L

N

N

K

S

K

R

L

L

V

L

D

A

V

L

A

A

K

D

E

S

D

D

V

A

I

I

V

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

T

T

D

D

E

A

V

V

I

M

E

D

G

G

A

P

S

A

S

S

V

A

I

V

F

W

D

D

Q

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

L

L

L

V

K

W

L

L

I

L

active site: R101 (= R102), H128 (= H129), Q131 (= Q132), D224 (= D220), C254 (= C260), R282 (= R288)
binding 5-methyl-4-phenyl-1,3-thiazol-2-amine: T51 (= T52), R52 (= R53), F55 (≠ V56)

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
43% identity, 98% coverage: 2:300/305 of query aligns to 40:342/354 of P00480

query
sites
P00480

K
x
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L

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A

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-

I

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Y

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K

K

G

G

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E

V

Y

T

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P

Y

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N

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E

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x
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S

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T

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T

R

R

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x
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x
F

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L

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N

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H

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G

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-

L

-

A

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A

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L

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x
E

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R

L

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Y

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T

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V

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C

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L

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A

-

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R

R

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E

E

E

I

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T

D

D

D

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E

V

V

I

F

E

Y

G

S

A

P

S

R

S

S

V

L

I

V

F

F

D

P

Q

E

A

A

E

E

N

N

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R

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W

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x
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A

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S

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I

L

R40 (≠ K2) to H: in OTCD; late onset; dbSNP:rs72554308
L43 (= L5) to F: in dbSNP:rs72554309
K46 (≠ S8) to R: in dbSNP:rs1800321
Y55 (≠ E17) to D: in OTCD; late onset; dbSNP:rs72554319
L63 (= L25) to P: in OTCD; late onset; dbSNP:rs72554324
K88 (≠ L49) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
STRT 90:93 (= STRT 51:54) binding carbamoyl phosphate
G100 (≠ A61) to D: in OTCD; late onset; dbSNP:rs72554349
F101 (≠ M62) to L: in dbSNP:rs1133135
L111 (= L72) to P: in dbSNP:rs1800324
T125 (≠ K86) to M: in OTCD; neonatal; dbSNP:rs72554356
D126 (= D87) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
R129 (= R90) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
A140 (≠ M101) to P: in OTCD; late onset; dbSNP:rs72556260
R141 (= R102) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
H168 (= H129) binding carbamoyl phosphate
Q171 (= Q132) binding carbamoyl phosphate
I172 (= I133) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
Y176 (≠ L137) to C: in OTCD; late onset; dbSNP:rs72556283
TL 178:179 (≠ TI 139:140) natural variant: Missing (in OTCD; neonatal)
Y183 (≠ K144) to D: in OTCD; late onset; dbSNP:rs72556292
G188 (≠ D149) to R: in OTCD; neonatal; dbSNP:rs72556294
G195 (= G156) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
D196 (= D157) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
L201 (≠ C162) to P: in OTCD; neonatal; dbSNP:rs72558407
S207 (≠ A168) to R: in OTCD; neonatal; dbSNP:rs72558415
A209 (= A170) to V: in OTCD; neonatal; dbSNP:rs72558417
M213 (= M174) to K: in OTCD; late onset
H214 (≠ E175) to Y: in OTCD; neonatal; dbSNP:rs72558420
P220 (= P181) to A: in OTCD; late onset; dbSNP:rs72558425
P225 (= P186) to T: in OTCD; late onset; dbSNP:rs72558428
L244 (= L201) to Q: in OTCD; late onset; dbSNP:rs72558436
T262 (= T219) to K: in OTCD; mild; dbSNP:rs67333670
T264 (≠ V221) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
W265 (= W222) to L: in OTCD; mild; dbSNP:rs72558446
G269 (= G226) to E: in OTCD; neonatal; dbSNP:rs72558450
Q270 (= Q227) to R: in dbSNP:rs1800328
E272 (≠ S229) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
R277 (= R234) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
H302 (= H259) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
C303 (= C260) to R: in OTCD; neonatal; dbSNP:rs67468335
CL 303:304 (= CL 260:261) binding carbamoyl phosphate
E309 (= E267) natural variant: Missing (in OTCD; late onset)
R330 (= R288) binding carbamoyl phosphate
T333 (≠ A291) natural variant: T -> A
S340 (≠ K298) to P: in OTCD; late onset; dbSNP:rs72558489

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
39 G → C: in OTCD; late onset; dbSNP:rs72554306
343 T → K: in OTCD; late onset; dbSNP:rs72558491

1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
42% identity, 98% coverage: 2:300/305 of query aligns to 7:309/321 of 1othA

query
sites
1othA

K

R

H

D

L

L

S

T

I

L

S

K

D

N

L

F

T

T

A

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E

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A

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W

L

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A

S

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A

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L

L

K

K

A

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E

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-

I

R

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Y

Q

K

K

G

G

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V

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T

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Y

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N

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S

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T

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R

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T

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S

F

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E

E

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T

A

G

M

F

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A

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L

L

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G

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H

A

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Y

F

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G

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x
V

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D

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A

A

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A

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L

A

A

A

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A

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P

V

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I

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N

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H

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A

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D

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Y

L

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T

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Y

G

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S

S

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L

E

K

D

G

V

L

K

T

V

L

A

S

W

W

V

I

G

G

D

D

G

G

N

N

N
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N

V

I

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N

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I

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M

A

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A

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V

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G

Y

Y

E

E

P

P

D

D

E

A

S

S

I

V

I

T

K

K

-

L

-

A

-

E

-

Q

K

Y

A

A

K

K

E

E

L

N

K

G

A

T

N

K

L

L

K

L

F

L

E

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S

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N

D

P

P

V

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A

A

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A

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G

A

G

D

N

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V

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D
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D

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T

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W

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S

M
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M

G

G

Q

R

E

E

S

E

E

E

K

K

R

K

K

K

R

R

L

L

K

Q

D

A

F

F

G

Q

P

G

Y

Y

Q

Q

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V

N

T

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M

K

K

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T

V

A

D

K

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V

A

A

K

A

E

S

D

D

V

W

I

T

V

F

M

L

H

H

C
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C

L
|
L

P

P

A

-

H

R

R

K

G

P

E

E

I

V

T

D

D

D

E

E

V

V

I

F

E

Y

G

S

A

P

S

R

S

S

V

L

I

V

F

F

D

P

Q

E

A

A

E

E

N

N

R
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R

L

K

H

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A

A

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active site: R59 (= R53), T60 (= T54), V87 (≠ R81), R108 (= R102), H135 (= H129), Q138 (= Q132), D230 (= D220), C270 (= C260), R297 (= R288)
binding n-(phosphonoacetyl)-l-ornithine: S57 (= S51), T58 (= T52), R59 (= R53), T60 (= T54), R108 (= R102), L130 (= L124), H135 (= H129), N166 (= N160), D230 (= D220), S234 (= S224), M235 (= M225), C270 (= C260), L271 (= L261), R297 (= R288)

1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
42% identity, 98% coverage: 2:300/305 of query aligns to 7:309/321 of 1c9yA

query
sites
1c9yA

K

R

H

D

L

L

S

T

I

L

S

K

D

N

L

F

T

T

A

G

E

E

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I

A

K

E

Y

I

M

L

L

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W

L

L

A

S

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A

K

D

L

L

K

K

A

F

E

R

-

I

R

K

Y

Q

K

K

G

G

V

E

V

Y

T

L

D

P

Y

L

L

L

K

Q

N

G

K

K

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S

L

L

A

G

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M

I

I

F

F

E

E

L

K

P

R

S
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S

T
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T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

E

E

V

T

A

G

M

F

T

A

D

L

L

L

G

G

H

H

A

P

L

C

Y

F

L

L

G

T

W

T

N

Q

E

D

L

I

Q

H

L

L

G

G

R
x
V

G

N

E

E

P

S

I

L

K

T

D

D

T

T

A

A

R

R

V

V

L

L

S

S

R

S

Y

M

V

A

H

D

A

A

I

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M

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M

A

R
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R

V

V

R

Y

N

K

H

Q

S

S

T

D

L

L

E

D

E

T

F

L

A

A

A

K

Y

E

S

A

S

S

V

I

P

P

V

I

I

I

N

N

G

G

L
|
L

S

S

N

D

L

L

E

Y

H
|
H

P

P

C

I

Q
|
Q

I

I

I

L

A

A

D

D

L

Y

L

L

T

T

I

L

K

Q

E

E

Y

H

K

Y

G

S

S

S

L

L

E

K

D

G

V

L

K

T

V

L

A

S

W

W

V

I

G

G

D

D

G

G

N

N

N
|
N

V

I

C

L

N

H

S

S

L

I

I

M

L

M

A

S

T

A

A

A

L

K

A

F

G

G

M

M

E

H

I

L

V

Q

V

A

S

A

T

T

P

P

S

K

G

G

Y

Y

E

E

P

P

D

D

E

A

S

S

I

V

I

T

K

K

-

L

-

A

-

E

-

Q

K

Y

A

A

K

K

E

E

L

N

K

G

A

T

N

K

L

L

K

L

F

L

E

T

S

N

N

D

P

P

V

L

K

E

A

A

V

A

K

H

D

G

A

G

D

N

V

V

I

L

Y

I

T

T

D
|
D

V

T

W

W

V

I

S
|
S

M
|
M

G

G

Q

R

E

E

S

E

E

E

K

K

R

K

K

K

R

R

L

L

K

Q

D

A

F

F

G

Q

P

G

Y

Y

Q

Q

V

V

N

T

K

M

K

K

L

T

V

A

D

K

V

V

A

A

K

A

E

S

D

D

V

W

I

T

V

F

M

L

H

H

C
|
C

L
|
L

P

P

A

-

H

R

R

K

G

P

E

E

I

V

T

D

D

D

E

E

V

V

I

F

E

Y

G

S

A

P

S

R

S

S

V

L

I

V

F

F

D

P

Q

E

A

A

E

E

N

N

R
|
R

L

K

H

W

A

T

Q

I

K

M

A

A

I

V

L

M

L

V

K

S

L

L

I

L

active site: R59 (= R53), T60 (= T54), V87 (≠ R81), R108 (= R102), H135 (= H129), Q138 (= Q132), D230 (= D220), C270 (= C260), R297 (= R288)
binding phosphoric acid mono(formamide)ester: S57 (= S51), T58 (= T52), R59 (= R53), T60 (= T54), R108 (= R102), C270 (= C260), L271 (= L261), R297 (= R288)
binding norvaline: L130 (= L124), N166 (= N160), D230 (= D220), S234 (= S224), M235 (= M225)

Query Sequence

>WP_013684049.1 NCBI__GCF_000194625.1:WP_013684049.1
MKHLLSISDLTAEELAEILELAEKLKAERYKGVVTDYLKNKSLAMIFELPSTRTRVSFEV
AMTDLGGHALYLGWNELQLGRGEPIKDTARVLSRYVHAIMMRVRNHSTLEEFAAYSSVPV
INGLSNLEHPCQIIADLLTIKEYKGSLEDVKVAWVGDGNNVCNSLILATALAGMEIVVST
PSGYEPDESIIKKAKELKANLKFESNPVKAVKDADVIYTDVWVSMGQESEKRKRLKDFGP
YQVNKKLVDVAKEDVIVMHCLPAHRGEEITDEVIEGASSVIFDQAENRLHAQKAILLKLI
GGEYD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory