SitesBLAST

7k5zA Crystal structure of argininosuccinate synthase from legionella pneumophila philadelphia 1 in complex with anppnp and a substrate analogue arginine
45% identity, 98% coverage: 2:388/393 of query aligns to 5:383/390 of 7k5zA

query
sites
7k5zA

K

K

V

I

V

A

L

L

S
x
A

Y
|
Y

S
|
S

G

G

L

L

D
|
D

T

T

T

S

V

I

C

M

I

I

P

P

L

W

L

L

K

K

E

E

K

H

Y

Y

G

E

F

H

D

A

E

E

V

V

V

I

T

A

V

V

T

I

V
x
C

D

D

I

L

G

G

Q

Q

P

Q

P

E

E

D

E

L

I

D

K

A

E

I

A

K

E

N

K

K

R

A

G

L

K

K

K

S

Y

G

A

A

D

S

K

K

H

A

Y

Y

T

V

I

V

D

D

A

V

K

K

E

N

E

E

F

F

-

A

V

T

R

Q

L

Y

L

L

F

W

R

P

L

L

I

V

K

K

A

S

N

G

G

A

D

L

Y

Y

E

E

G

D

-

Q

Y
|
Y

V

I

L

L

G

G

T
|
T

S

-

L

I

A

S

R
|
R

P

P

L

L

I

I

A

A

E

Q

K

K

I

L

V

V

E

E

V

I

A

A

K

L

K

T

E

E

N

Q

A

V

D

N

A

A

I

V

A

A

H

H

G
|
G

C

A

T

T

G

G

K

K

G

G

N

N

D
|
D

Q

Q

L

V

R
|
R

F
|
F

E

E

N

Y

V

S

F

I

Y

K

Q

A

Y

L

G

A

-

P

-

Q

F

L

R

E

V

I

I

I

A

A

P

P

M

W

R

R

E

T

L

W

N

D

L

I

-

K

T

S

R

R

E

Q

W

E

E

A

I

I

E

V

Y

Y

A

A

K

K

Q

A

H

H

G

G

I

I

E

E

V

V

P

P

V

V

T

T

K

P

E

K

K

A

P

P

Y

Y

S
|
S

I

R

D

D

E

H

N

N

L

I

W

W

S

Y

R

I

S
|
S

I

H

E
|
E

G

G

K

V

L

L

E

E

D

D

P

P

S

S

F

Q

E

E

P

M

P

P

E

N

D

D

I

V

F

L

E

L

W

M

T

T

A

A

S

P

P

V

A

S

K

Q

A

T

P

P

D

D

E

E

P

E

E

E

K

V

V

V

E

V

I

L

Y

D

F

F

E

K

Q

K

G

G

V

V

P

P

V

V

A

A

I

L

N

N

G

G

E

Q

R

E

L

L

P

S

G

P

L

V

E

D

L

L

I

L

R

N

K

S

L

L

N

N

E

Q

I

K

G

A

G

G

R

Q

H

H

G

G

V

I

G

G

R

V

T

A

D

D

M

I

M

V

E
|
E

D

N

R

R

V

L

L

V

G

G

L

M

K

K

A

I

R

R

E

G

N

I

Y
|
Y

E

E

H

A

P

P

A

A

T

A

I

V

L

L

I

Y

T

K

A

A

H

H

R

K

D

L

L

L

E

E

K

S

L

L

V

C

L

L

S

T

R

R

R

S

E

T

L

L

K

H

F

L

K

K

K

Q

F

S

V

L

E

Q

E

T

W

Y

A

A

E

N

L

L

V

V

Y

Y

Y

E

G

G

L

R

T

W

N

F

D

S

P

Q

L

T

F

K

D

Q

A

A

L

L

N

D

A

A

F

F

I

I

D

D

E

V

T

T

Q

Q

K

Q

R

H

V

V

T

T

G

G

W

C

V

V

R

K

L

L

K

K

L

L

F

F

K

K

G

G

S

N

V

I

T

I

P

P

V

A

A

G

R

M

Y

H

S

S

E

P

F

Y

A

S

L

L

Y

H

R

H

E

-

L

-

V

-

S

-

F

-

D

-

T

-

A

-

I

-

D

-

Q

Q

K

K

Y

D

A

A

E

E

G

G

F

F

S

I

R

N

F

L

H

F

G

S

L

L

Q

S

G

A

R

K

I

I

Y

Y

R

S

E

Q

I

V

active site: D15 (= D12), R95 (= R91), D124 (= D120), S176 (= S169)
binding phosphoaminophosphonic acid-adenylate ester: A9 (≠ S6), Y10 (= Y7), S11 (= S8), C37 (≠ V34), G117 (= G113), F128 (= F124)
binding arginine: Y88 (= Y83), T92 (= T87), D124 (= D120), R127 (= R123), S185 (= S178), E187 (= E180), E261 (= E254), Y273 (= Y266)

1j20A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with product (see paper)
51% identity, 98% coverage: 1:384/393 of query aligns to 1:380/386 of 1j20A

query
sites
1j20A

M

M

K

K

V

I

V

V

L

L

S
x
A

Y

Y

S

S

G

G

L

L

D
|
D

T
|
T

T

S

V

I

C

I

I

L

P

K

L

W

L

L

K

K

E

E

K

T

Y

Y

G

R

F

-

D

A

E

E

V

V

V

I

T

A

V

F

T

T

V
x
A

D

D

I

I

G

G

Q

Q

P

G

P

-

E

E

I

V

K

E

E

E

A

A

-

R

E

E

K

K

R

A

G

L

K

R

K

T

Y

G

A

A

D

S

K

K

H

A

Y

I

T

A

I

L

D

D

A

L

K

K

E

E

F

F

V

V

R

R

-

D

L

F

L

V

F

F

R

P

L

M

I

M

K

R

A

A

N

G

G

A

D

V

Y

Y

E

E

G

G

Y

Y

-
x
Y

V

L

L

L

G

G

T
|
T

S

S

L

I

A

A

R
|
R

P

P

L

L

I

I

A

A

E

K

K

H

I

L

V

V

E

R

V

I

A

A

K

E

E

E

N

G

A

A

D

E

A

A

I

I

A

A

H
|
H

G
|
G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F
|
F

E

E

N

L

V

T

F

A

Y

Y

Q

A

Y

L

-

K

-

P

G

D

F

I

R

K

V

V

I

I

A

A

P

P

M

W

R

R

E

E

L

W

N

S

L

F

T

Q

-

G

R

R

E

K

W

E

E

M

I

I

E

A

Y

Y

A

A

K

E

Q

A

H

H

G

G

I

I

E

P

V

V

P

P

V

-

T

-

K

-

E

-

K

-

P

P

Y

Y

S
|
S

I

M

D

D

E

A

N

N

L

L

W

L

S

H

R

I

S
|
S

I

Y

E
|
E

G

G

K

V

L

L

E

E

D

D

P

P

S

W

F

A

E

E

P

P

E

K

D

G

I

M

F

F

E

R

W

M

T

T

A

Q

S

D

P

P

A

E

K

E

A

A

P

P

D

D

E

A

P

P

E

E

K

Y

V

V

E

E

I

V

Y

E

F

F

E

F

Q

E

G

G

V

D

P

P

V

V

A

A

I

V

N

N

G

G

E

E

R

R

L

L

P

S

G

P

L

A

E

A

L

L

I

L

R

Q

K

R

L

L

N

N

E

E

I

I

G

G

R

R

H

H

G

G

V

V

G

G

R

R

T

V

D

D

M

I

M

V

E
|
E

D

N

R

R

V

F

L

V

G

G

L

M

K

K

A

S

R

R

E

G

N

V

Y
|
Y

E

E

H

T

P

P

A

G

T

T

I

I

L

L

I

Y

T

H

A

A

H

R

R

R

D

A

L

V

E

E

K

S

L

L

V

T

L

L

S

D

R

R

R

E

E

V

L

L

K

H

F

Q

K

R

K

D

F

M

V

L

E

S

E

P

E

K

W

Y

A

A

E

E

L

L

V

V

Y

Y

G

G

L

F

T

W

N

Y

D

A

P

P

L

E

F

R

D

E

A

A

L

L

N

Q

A

A

F

Y

I

F

D

D

E

H

T

V

Q

A

K

R

R

S

V

V

T

T

G

G

W

V

V

A

R

R

L

L

K

K

L

L

F

Y

K

K

G

G

S

N

V

V

T

Y

P

V

V

V

A

G

R

R

Y

K

S

A

E

P

F

K

A

S

L

L

Y

Y

R

R

E

Q

E

D

L

L

V

V

S

S

F

F

D

-

T

-

A

G

I

Y

D

D

Q

Q

K

K

Y

D

A

A

E

E

G

G

F

F

S

I

R

K

F

I

H

Q

G

A

L

L

Q

R

G

L

R

R

I

V

active site: D12 (= D12), R92 (= R91), D121 (= D120), S168 (= S169)
binding adenosine monophosphate: A6 (≠ S6), T13 (= T13), A33 (≠ V34), R92 (= R91), H113 (= H112), G114 (= G113), F125 (= F124)
binding argininosuccinate: Y84 (vs. gap), T88 (= T87), A115 (≠ C114), T116 (= T115), G119 (= G118), N120 (= N119), D121 (= D120), R124 (= R123), S177 (= S178), E179 (= E180), E253 (= E254), Y265 (= Y266)

1j1zA Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with substrate (see paper)
51% identity, 98% coverage: 1:384/393 of query aligns to 1:380/386 of 1j1zA

query
sites
1j1zA

M

M

K

K

V

I

V

V

L

L

S
x
A

Y

Y

S

S

G

G

L

L

D
|
D

T
|
T

T

S

V

I

C

I

I

L

P

K

L

W

L

L

K

K

E

E

K

T

Y

Y

G

R

F

-

D

A

E

E

V

V

V

I

T

A

V

F

T

T

V
x
A

D

D

I

I

G

G

Q

Q

P

G

P

-

E

E

I

V

K

E

E

E

A

A

-

R

E

E

K

K

R

A

G

L

K

R

K

T

Y

G

A

A

D

S

K

K

H

A

Y

I

T

A

I

L

D

D

A

L

K

K

E

E

F

F

V

V

R

R

-

D

L

F

L

V

F

F

R

P

L

M

I

M

K

R

A

A

N

G

G

A

D

V

Y

Y

E

E

G

G

Y

Y

-
x
Y

V

L

L

L

G

G

T
|
T

S

S

L

I

A

A

R
|
R

P

P

L

L

I
|
I

A

A

E

K

K

H

I

L

V

V

E

R

V

I

A

A

K

E

E

E

N

G

A

A

D

E

A

A

I

I

A

A

H
|
H

G
|
G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F
|
F

E

E

N

L

V

T

F

A

Y

Y

Q

A

Y

L

-

K

-

P

G

D

F

I

R

K

V

V

I

I

A

A

P

P

M

W

R

R

E

E

L

W

N

S

L

F

T

Q

-

G

R

R

E

K

W

E

E

M

I

I

E

A

Y

Y

A

A

K

E

Q

A

H

H

G

G

I

I

E

P

V

V

P

P

V

-

T

-

K

-

E

-

K

-

P

P

Y

Y

S
|
S

I
x
M

D

D

E

A

N

N

L

L

W

L

S

H

R

I

S
|
S

I

Y

E
|
E

G

G

K

V

L

L

E

E

D

D

P

P

S

W

F

A

E

E

P

P

E

K

D

G

I

M

F

F

E

R

W

M

T

T

A

Q

S

D

P

P

A

E

K

E

A

A

P

P

D

D

E

A

P

P

E

E

K

Y

V

V

E

E

I

V

Y

E

F

F

E

F

Q

E

G

G

V

D

P

P

V

V

A

A

I

V

N

N

G

G

E

E

R

R

L

L

P

S

G

P

L

A

E

A

L

L

I

L

R

Q

K

R

L

L

N

N

E

E

I

I

G

G

R

R

H

H

G

G

V

V

G

G

R

R

T

V

D

D

M

I

M

V

E
|
E

D

N

R

R

V

F

L

V

G

G

L

M

K

K

A

S

R

R

E

G

N

V

Y
|
Y

E

E

H

T

P

P

A

G

T

T

I

I

L

L

I

Y

T

H

A

A

H

R

R

R

D

A

L

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K

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L

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T

L

L

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D

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R

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R

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D

F

M

V

L

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S

E

P

E

K

W

Y

A

A

E

E

L

L

V

V

Y

Y

G

G

L

F

T

W

N

Y

D

A

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P

L

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F

R

D

E

A

A

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L

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Q

A

A

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F

D

D

E

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Q

A

K

R

R

S

V

V

T

T

G

G

W

V

V

A

R

R

L

L

K

K

L

L

F

Y

K

K

G

G

S

N

V

V

T

Y

P

V

V

V

A

G

R

R

Y

K

S

A

E

P

F

K

A

S

L

L

Y

Y

R

R

E

Q

E

D

L

L

V

V

S

S

F

F

D

-

T

-

A

G

I

Y

D

D

Q

Q

K

K

Y

D

A

A

E

E

G

G

F

F

S

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R

K

F

I

H

Q

G

A

L

L

Q

R

G

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R

I

V

active site: D12 (= D12), R92 (= R91), D121 (= D120), S168 (= S169)
binding aspartic acid: A115 (≠ C114), T116 (= T115), G119 (= G118), N120 (= N119), D121 (= D120)
binding adenosine-5'-triphosphate: A6 (≠ S6), T13 (= T13), A33 (≠ V34), R92 (= R91), I95 (= I94), H113 (= H112), G114 (= G113), F125 (= F124)
binding citrulline: Y84 (vs. gap), T88 (= T87), R124 (= R123), S168 (= S169), M169 (≠ I170), S177 (= S178), E179 (= E180), E253 (= E254), Y265 (= Y266)

1kh3A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with inhibitor (see paper)
49% identity, 98% coverage: 1:384/393 of query aligns to 1:374/380 of 1kh3A

query
sites
1kh3A

M

M

K

K

V

I

V

V

L

L

S
x
A

Y

Y

S

S

G

G

L

L

D
|
D

T
|
T

T

S

V

I

C

I

I

L

P

K

L

W

L

L

K

K

E

E

K

T

Y

Y

G

R

F

-

D

A

E

E

V

V

V

I

T

A

V

F

T
|
T

V
x
A

D

D

I

I

G

G

Q

Q

P

G

P

-

E

E

I

V

K

E

E

E

A

A

-

R

E

E

K

K

R

A

G

L

K

R

K

T

Y

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active site: D12 (= D12), R92 (= R91), D121 (= D120), S168 (= S169)
binding phosphoaminophosphonic acid-adenylate ester: A6 (≠ S6), T13 (= T13), T32 (= T33), A33 (≠ V34), H113 (= H112), G114 (= G113), F125 (= F124), S168 (= S169), M169 (≠ I170)
binding arginine: Y84 (vs. gap), T88 (= T87), R124 (= R123), S168 (= S169), M169 (≠ I170), D170 (= D171), S177 (= S178), E179 (= E180), E253 (= E254), Y265 (= Y266)
binding aspartic acid: A115 (≠ C114), T116 (= T115), G119 (= G118), N120 (= N119), D121 (= D120)

4xfjB Crystal structure of argininosuccinate synthase from mycobacterium thermoresistibile in complex with amppnp and arginine
41% identity, 97% coverage: 2:384/393 of query aligns to 3:390/397 of 4xfjB

query
sites
4xfjB

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active site: D13 (= D12), R94 (= R91), D123 (= D120), S174 (= S169)
binding phosphoaminophosphonic acid-adenylate ester: A7 (≠ S6), Y8 (= Y7), S9 (= S8), T14 (= T13), I34 (≠ V34), G116 (= G113), C117 (= C114), F127 (= F124)
binding arginine: Y86 (= Y83), S90 (≠ T87), R126 (= R123), A183 (≠ S178), E185 (= E180), E259 (= E254), E269 (= E264), Y271 (= Y266)

P00966 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Homo sapiens (Human) (see 16 papers)
42% identity, 99% coverage: 3:391/393 of query aligns to 7:408/412 of P00966

query
sites
P00966

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V64 (≠ A62) to I: in CTLN1; uncertain significance; dbSNP:rs556297791
Y87 (= Y83) binding L-citrulline
T91 (= T87) to P: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs769018733
S92 (= S88) binding L-citrulline
R95 (= R91) to S: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity
P96 (= P92) to H: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; to L: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; loss of argininosuccinate synthase activity; to S: in CTLN1; no effect on thermal stability; decreased argininosuccinate synthase activity
G117 (= G113) to S: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770944877
A118 (≠ C114) to T: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs775305020
T119 (= T115) binding L-aspartate; to I: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity
N123 (= N119) binding L-aspartate; binding L-citrulline
D124 (= D120) binding L-aspartate; to N: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs936192871
R127 (= R123) binding L-citrulline; to L: increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to Q: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to W: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs771794639
R157 (vs. gap) to C: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770585183; to H: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908637
K165 (= K154) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-176 or R-176.
K176 (≠ E165) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-165 or R-165.
W179 (≠ Y168) to R: in CTLN1; mild; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908646
S180 (= S169) binding L-citrulline; to I: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs121908638; to N: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908638
S189 (= S178) binding L-citrulline
E191 (= E180) to Q: in CTLN1; loss of argininosuccinate synthase activity
A192 (≠ G181) to V: in CTLN1; decreased protein abundance
V263 (= V247) to M: in CTLN1; mild clinical course; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs192838388
R265 (= R249) to C: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs148918985
E270 (= E254) binding L-citrulline; to Q: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs775163147
R272 (= R256) to C: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs762387914; to H: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008; to L: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008
G280 (≠ E264) to R: in CTLN1; loss of argininosuccinate synthase activity
Y282 (= Y266) binding L-citrulline
T284 (≠ H268) to I: in CTLN1; mild clinical course; dbSNP:rs886039853
M302 (≠ L286) to V: in CTLN1; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity
R304 (= R288) to W: in CTLN1; decreased protein abundance; dbSNP:rs121908642
G324 (= G308) to S: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908639
G347 (= G331) to R: in CTLN1; severe clinical course
Y359 (≠ T343) to D: in CTLN1; mild clinical course
G362 (≠ A346) to V: in CTLN1; mild; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908647
G390 (= G373) to R: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908641

2nz2A Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline (see paper)
42% identity, 98% coverage: 3:388/393 of query aligns to 4:400/402 of 2nz2A

query
sites
2nz2A

V

V

L

L

S

A

Y

Y

S

S

G

G

L

L

D
|
D

T

T

T

S

V

C

C

I

I

L

P

V

L

W

L

L

K

K

E

E

K

Q

Y

-

G

G

F

Y

D

D

E

-

V

V

V

I

T

A

V

Y

T

L

V

A

D

N

I

I

G

G

Q

Q

P

K

P

-

E

E

E

D

I

F

K

E

E

E

A

A

E

R

K

K

R

K

G

A

K

L

K

K

Y

L

A

G

D

A

K

K

H

K

Y

V

T

F

I

I

-

E

D

D

A

V

K

S

E

R

E

E

F

F

V

V

R

E

-

E

L

F

L

I

F

W

R

P

L

A

I

I

K

Q

A

S

N

S

G

A

D

L

Y

Y

E

E

G

D

-

R

Y
|
Y

V

L

L

L

G

G

T
|
T

S

S

L

L

A

A

R
|
R

P

P

L

C

I

I

A

A

E

R

K

K

I

Q

V

V

E

E

V

I

A

A

K

Q

K

R

E

E

N

G

A

A

D

K

A

Y

I

V

A

S

H

H

G

G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F

F

E

E

N

L

V

S

F

C

Y

Y

Q

S

Y

L

G

A

-

P

-

Q

F

I

R

K

V

V

I

I

A

A

P

P

M

W

R

R

E

M

L

P

N

E

L

F

T

Y

R

N

E

R

W

F

E

K

-

R

-

N

-

D

-

L

I

M

E

E

Y

Y

A

A

K

K

Q

Q

H

H

G

G

I

I

E

P

V

I

P

P

V

V

T

T

K

P

E

K

K

N

P

P

Y

W

S
|
S

I

M

D
|
D

E

E

N

N

L

L

W

M

S

H

R

I

S
|
S

I

Y

E
|
E

G

A

G

G

K

I

L

L

E

E

D

N

P

P

S

K

F

N

E

Q

P

A

P

P

E

P

D

G

I

L

F

Y

E

T

W

K

T

T

A

Q

S

D

P

P

A

A

K

K

A

A

P

P

D

N

E

T

P

P

E

D

K

I

V

L

E

E

I

I

Y

E

F

F

E

K

Q

K

G

G

V

V

P

P

V

V

A

K

I

V

N

T

G

N

E

V

R

K

-

D

-

G

-

T

-

H

L

Q

P

T

G

S

L

L

E

E

L

L

I

F

R

M

K

Y

L

L

N

N

E

E

I

V

G

A

G

G

R

K

H

H

G

G

V

V

G

G

R

R

T

I

D

D

M

I

M

V

E
|
E

D

N

R

R

V

F

L

I

G

G

L

M

K

K

A

S

R

R

E

G

N

I

Y
|
Y

E

E

H

T

P

P

A

A

A

G

T

T

I

I

L

L

I

Y

T

H

A

A

H

H

R

L

D

D

L

I

E

E

K

A

L

F

V

T

L

M

S

D

R

R

R

E

E

V

L

R

K

K

F

I

K

K

K

Q

F

G

V

L

E

G

E

L

E

K

W

F

A

A

E

E

L

L

V

V

Y

Y

Y

T

G

G

L

F

T

W

N

H

D

S

P

P

L

E

F

C

D

E

A

F

L

V

N

R

A

H

F

C

I

I

D

A

E

K

T

S

Q

Q

K

E

R

R

V

V

T

E

G

G

W

K

V

V

R

Q

L

V

K

S

L

V

F

L

K

K

G

G

S

Q

V

V

T

Y

P

I

V

L

A

G

R

R

Y

E

S

S

E

P

F

L

A

S

L

L

Y

Y

R

N

E

E

L

L

V

V

S

S

F

N

D

V

T

Q

T

G

A

D

I

Y

D

E

Q

P

K

T

Y

D

A

A

E

T

G

G

F

F

S

I

R

N

F

I

H

N

G

S

L

L

Q

R

G

L

R

K

I

E

Y

Y

R

H

E

R

I

L

active site: D13 (= D12), R92 (= R91), D121 (= D120), S176 (= S169)
binding aspartic acid: A115 (≠ C114), T116 (= T115), G119 (= G118), N120 (= N119), D121 (= D120)
binding citrulline: Y84 (= Y83), T88 (= T87), N120 (= N119), R124 (= R123), D178 (= D171), S185 (= S178), E187 (= E180), E266 (= E254), Y278 (= Y266)

5us8A 2.15 angstrom resolution crystal structure of argininosuccinate synthase from bordetella pertussis
28% identity, 87% coverage: 2:341/393 of query aligns to 16:371/445 of 5us8A

query
sites
5us8A

K

K

V

V

V

G

L

I

S

A

Y

F

S

S

G

G

L

L

D
|
D

T

T

T

S

V

A

C

A

I

L

P

L

L

W

L

M

K

R

E

Q

K

K

Y

G

G

A

F

V

D

P

E

-

V

-

V

Y

T

A

V

Y

T

T

V

A

D

N

I

L

G

G

Q

Q

P

P

P

D

E

E

-

P

E

D

I

Y

K

D

E

E

A

I

E

P

K

R

R

R

G

A

K

M

K

Q

Y

Y

-

G

A

A

D

E

K

A

H

A

Y

R

T

L

I

V

D

D

A

C

K

R

E

A

E

Q

F

L

V

V

R

A

L

E

L

G

F

I

R

A

L

A

I

L

K

Q

A

A

N

G

G

A

D

F

Y

H

E

I

G

S

-

T

-

A

-

G

-

L

-

T

Y

Y

V

F

L

N

G

T

T

T

S

P

L

I

A

G

R
|
R

P

A

L

V

I

T

A

G

E

T

K

M

I

L

V

V

E

A

V

A

A

M

K

K

K

E

E

D

N

G

A

V

D

N

A

I

I

W

A

G

H

D

G

G

C

S

T

T

G

F

K

K

G

G

N

N

D
|
D

Q

I

L

E

R

R

F

F

E

-

N

-

V

-

F

-

Y

Y

Q

R

Y

Y

G

G

F

L

R

-

V

L

I

T

A

N

P

P

M

-

R

-

E

D

L

L

N

K

L

I

T

Y

R

K

E

P

W

W

-

L

-

D

-

Q

-

T

-

F

-

I

-

D

-

E

-

L

-

G

-

R

-

A

-

E

E

M

I

S

E

E

Y

Y

A

M

K

R

Q

Q

H

A

G

G

I

F

E

D

V

Y

P

K

V

M

T

S

K

A

E

E

K

K

P

A

Y

Y

S
|
S

I

T

D

D

E

S

N

N

L

M

W

L

S

G

R

A

S

T

I

H

E

E

G

A

G

K

K

D

L

L

E

E

D

L

P

L

S

S

-

A

-

G

-

I

-

R

-

I

F

V

E

Q

P

P

P

I

E

M

D

G

I

V

F

A

E

F

W

W

T

Q

A

D

S

S

P

-

A

-

K

-

A

V

P

Q

D

I

E

K

P

A

E

E

K

E

V

V

E

T

I

V

Y

R

F

F

E

E

Q

E

G

G

V

Q

P

P

V

V

A

A

I

L

N

N

G

G

-

V

E

E

R

Y

L

A

P

D

G

P

L

V

E

E

L

L

I

L

R

L

K

E

L

A

N

N

E

R

I

I

G

G

R

R

H

H

G

G

V

L

G

G

R

M

T

S

D

D

M

Q

M

I

E

E

D

N

R

R

V

I

L

I

G

E

L

A

K

K

A

S

R

R

E

G

N

I

Y

Y

E

E

H

A

P

P

A

G

A

L

T

A

I

L

L

L

I

F

T

I

A

A

H

Y

R

E

D

R

L

L

E

V

K

T

L

G

V

I

L

H

S

N

R

E

R

D

E

T

L

I

K
x
E

F

Q

K

Y

K
x
R

F
x
E

V

N

E

G

E
x
R

E
x
K

W

L

A

G

E

R

L

L

V

L

Y

Y

Y

Q

G

G

L

-

T

-

N

-

D

-

P

R

L

W

F

F

D

D

A

P

L

Q

N

A

A

I

F

M

I

L

D

R

E

E

T

T

Q

A

K

Q

R

R

-

W

-

V

-

A

-

R

-

A

V

I

T

T

G

G

W

E

V

V

R

T

L

L

K

E

L

L

F

R

K

R

G

G

S

N

active site: D26 (= D12), R110 (= R91), D139 (= D120), S195 (= S169)
binding adenosine: E321 (≠ K292), R324 (≠ K295), E325 (≠ F296), R328 (≠ E299), K329 (≠ E300)

6e5yA 1.50 angstrom resolution crystal structure of argininosuccinate synthase from bordetella pertussis in complex with amp.
28% identity, 87% coverage: 2:341/393 of query aligns to 12:367/438 of 6e5yA

query
sites
6e5yA

K

K

V

V

V

G

L

I

S
x
A

Y

F

S

S

G

G

L

L

D
|
D

T

T

T

S

V

A

C

A

I

L

P

L

L

W

L

M

K

R

E

Q

K

K

Y

G

G

A

F

V

D

P

E

-

V

-

V

Y

T

A

V

Y

T
|
T

V
x
A

D

N

I

L

G

G

Q

Q

P

P

P

D

E

E

-

P

E

D

I

Y

K

D

E

E

A

I

E

P

K

R

R

R

G

A

K

M

K

Q

Y

Y

-

G

A

A

D

E

K

A

H

A

Y

R

T

L

I

V

D

D

A

C

K

R

E

A

E

Q

F

L

V

V

R

A

L

E

L

G

F

I

R

A

L

A

I

L

K

Q

A

A

N

G

G

A

D

F

Y

H

E

I

G

S

-

T

-

A

-

G

-

L

-

T

Y

Y

V

F

L

N

G

T

T

T

S

P

L

I

A

G

R
|
R

P

A

L

V

I

T

A

G

E

T

K

M

I

L

V

V

E

A

V

A

A

M

K

K

K

E

E

D

N

G

A

V

D

N

A

I

I

W

A

G

H

D

G

G

C

S

T

T

G

F

K

K

G

G

N

N

D
|
D

Q

I

L

E

R

R

F

F

E

-

N

-

V

-

F

-

Y

Y

Q

R

Y

Y

G

G

F

L

R

-

V

L

I

T

A

N

P

P

M

-

R

-

E

D

L

L

N

K

L

I

T

Y

R

K

E

P

W

W

-

L

-

D

-

Q

-

T

-

F

-

I

-

D

-

E

-

L

-

G

-

R

-

A

-

E

E

M

I

S

E

E

Y

Y

A

M

K

R

Q

Q

H

A

G

G

I

F

E

D

V

Y

P

K

V

M

T

S

K

A

E

E

K

K

P

A

Y

Y

S
|
S

I

T

D
|
D

E

S

N

N

L

M

W

L

S

G

R

A

S

T

I

H

E

E

G

A

G

K

K

D

L

L

E

E

D

L

P

L

S

S

-

A

-

G

-

I

-

R

-

I

F

V

E

Q

P

P

P

I

E

M

D

G

I

V

F

A

E

F

W

W

T

Q

A

D

S

S

P

-

A

-

K

-

A

V

P

Q

D

I

E

K

P

A

E

E

K

E

V

V

E

T

I

V

Y

R

F

F

E

E

Q

E

G

G

V

Q

P

P

V

V

A

A

I

L

N

N

G

G

-

V

E

E

R

Y

L

A

P

D

G

P

L

V

E

E

L

L

I

L

R

L

K

E

L

A

N

N

E

R

I

I

G

G

R

R

H

H

G

G

V

L

G

G

R

M

T

S

D

D

M

Q

M

I

E

E

D

N

R

R

V

I

L

I

G

E

L

A

K

K

A

S

R

R

E

G

N

I

Y

Y

E

E

H

A

P

P

A

G

A

L

T

A

I

L

L

L

I

F

T

I

A

A

H

Y

R

E

D

R

L

L

E

V

K

T

L

G

V

I

L

H

S

N

R

E

R

D

E

T

L

I

K

E

F

Q

K

Y

K

R

F

E

V

N

E

G

E

R

E

K

W

L

A

G

E

R

L

L

V

L

Y

Y

Y

Q

G

G

L

-

T

-

N

-

D

-

P

R

L

W

F

F

D

D

A

P

L

Q

N

A

A

I

F

M

I

L

D

R

E

E

T

T

Q

A

K

Q

R

R

-

W

-

V

-

A

-

R

-

A

V

I

T

T

G

G

W

E

V

V

R

T

L

L

K

E

L

L

F

R

K

R

G

G

S

N

active site: D22 (= D12), R106 (= R91), D135 (= D120), S191 (= S169)
binding adenosine monophosphate: A16 (≠ S6), T41 (= T33), A42 (≠ V34), D193 (= D171)

1k97A Crystal structure of e. Coli argininosuccinate synthetase in complex with aspartate and citrulline (see paper)
28% identity, 91% coverage: 2:359/393 of query aligns to 12:386/432 of 1k97A

query
sites
1k97A

K

R

V

I

V

G

L

I

S

A

Y

F

S

S

G

G

L

L

D
|
D

T

T

T

S

V

A

C

A

I

L

P

L

L

W

L

M

K

R

E

Q

K

K

Y

G

G

A

F

V

D

P

E

-

V

-

V

Y

T

A

V

Y

T

T

V

A

D

N

I

L

G

G

Q

Q

P

P

P

D

E

E

I

D

K

Y

E

D

A

A

-

I

E

P

K

R

R

R

G

A

K

M

K

E

Y

Y

-

G

A

A

D

E

K

N

H

A

Y

R

T

L

I

I

D

D

A

C

K

R

E

K

E

Q

F

L

V

V

R

A

L

E

L

G

F

I

R

A

L

A

I

I

K

Q

A

C

N

G

G

A

D

F

Y

H

E

N

G

T

-

T

-

G

-

L

-

T

Y
|
Y

V

F

L

N

G

T

T
|
T

S
x
P

L

L

A

G

R
|
R

P

A

L

V

I

T

A

G

E

T

K

M

I

L

V

V

E

A

V

A

A

M

K

K

K

E

E

D

N

G

A

V

D

N

A

I

I

W

A

G

H

D

G

G

C
x
S

T
|
T

G

Y

K

K

G
|
G

N
|
N

D
|
D

Q

I

L

E

R
|
R

F

F

E

-

N

-

V

-

F

-

Y

Y

Q

R

Y

Y

G

G

F

L

R

L

V

T

I

N

A

A

P

-

M

-

R

-

E

E

L

L

N

Q

L

I

T

Y

R

K

E

P

W

W

-

L

-

D

-

T

-

D

-

F

-

I

-

D

-

E

-

L

-

G

-

R

-

H

-

E

E

M

I

S

E

E

Y

F

A

M

K

I

Q

A

H

C

G

G

I

F

E

D

V

Y

P

K

V

M

T

S

K

V

E

E

K

K

P

A

Y

Y

S
|
S

I
x
T

D
|
D

E

S

N

N

L

M

W

L

S

G

R

A

S
x
T

I

H

E
|
E

G

A

G

K

K

D

L

L

E

E

-

Y

-

L

D

N

P

S

S

S

F

V

E

K

-

I

-

V

-

N

P

P

P

I

E

M

D

G

I

V

F

K

E

F

W

W

T

D

A

E

S

S

P

-

A

V

K

K

A

I

P

P

D

-

E

-

P

A

E

E

K

E

V

V

E

T

I

V

Y

R

F

F

E

E

Q

Q

G

G

V

H

P

P

V

V

A

A

I

L

N

N

G

G

E

K

R

T

L

F

P

S

G

D

-

D

L

V

E

E

L

M

I

M

R

L

K

E

L

A

N

N

E

R

I

I

G

G

R

R

H

H

G

G

V

L

G

G

R

M

T

S

D

D

M

Q

M

I

E
|
E

D

N

R

R

V

I

L

I

G

E

L

A

K

K

A

S

R

R

E

G

N

I

Y
|
Y

E

E

H

A

P

P

A

G

A

M

T

A

I

L

L

L

I

H

T

I

A

A

H

Y

R

E

D

R

L

L

E

L

K

T

L

G

V

I

L

H

S

N

R

E

R

D

E

T

L

I

K

E

F

Q

K

Y

K

H

F

A

V

H

E

G

E

R

E

Q

W

L

A

G

E

R

L

L

V

L

Y
|
Y

Y

Q

G

G

L

R

T

W

N

F

D

D

P

S

-

Q

-

A

-

L

-

M

L

L

F

R

D

D

A

S

L

L

N

Q

A

R

F

W

I

V

D

-

E

-

T

-

Q

A

K

S

R

Q

V

I

T

T

G

G

W

E

V

V

R

T

L

L

K

E

L

L

F

R

K

R

G

G

S

N

-

D

V

Y

T

S

P

I

V

L

A

N

R

T

Y

V

S

S

E

E

F

N

A

L

L

T

Y

Y

R

K

E

P

E

E

L

S

V

V

active site: D22 (= D12), R106 (= R91), D135 (= D120), S191 (= S169)
binding aspartic acid: S129 (≠ C114), T130 (= T115), G133 (= G118), N134 (= N119), D135 (= D120)
binding citrulline: Y98 (= Y83), T102 (= T87), P103 (≠ S88), R138 (= R123), S191 (= S169), T192 (≠ I170), D193 (= D171), T200 (≠ S178), E202 (= E180), E279 (= E254), Y291 (= Y266), Y331 (= Y306)

1kp3A Crystal structure of e. Coli argininosuccinate synthetase in complex with atp and citrulline (see paper)
28% identity, 87% coverage: 2:341/393 of query aligns to 12:367/439 of 1kp3A

query
sites
1kp3A

K

R

V

I

V

G

L

I

S
x
A

Y

F

S
|
S

G

G

G
|
G

L

L

D
|
D

T
|
T

T

S

V

A

C

A

I

L

P

L

L

W

L

M

K

R

E

Q

K

K

Y

G

G

A

F

V

D

P

E

-

V

-

V

Y

T

A

V

Y

T
|
T

V
x
A

D

N

I

L

G

G

Q

Q

P

P

P

D

E

E

I

D

K

Y

E

D

A

A

-

I

E

P

K

R

R

R

G

A

K

M

K

E

Y

Y

-

G

A

A

D

E

K

N

H

A

Y

R

T

L

I

I

D

D

A

C

K

R

E

K

E

Q

F

L

V

V

R

A

L

E

L

G

F

I

R

A

L

A

I

I

K

Q

A

C

N

G

G

A

D

F

Y

H

E

N

G

T

-

T

-

G

-

L

-

T

Y
|
Y

V

F

L

N

G

T

T
|
T

S
x
P

L

L

A

G

R
|
R

P

A

L

V

I

T

A

G

E

T

K

M

I

L

V

V

E

A

V

A

A

M

K

K

K

E

E

D

N

G

A

V

D

N

A

I

I

W

A

G

H
x
D

G
|
G

C
x
S

T
|
T

G

Y

K

K

G
|
G

N
|
N

D
|
D

Q

I

L

E

R
|
R

F
|
F

E

-

N

-

V

-

F

-

Y

Y

Q

R

Y

Y

G

G

F

L

R

L

V

T

I

N

A

A

P

-

M

-

R

-

E

E

L

L

N

Q

L

I

T

Y

R

K

E

P

W

W

-

L

-

D

-

T

-

D

-

F

-

I

-

D

-

E

-

L

-

G

-

R

-

H

-

E

E

M

I

S

E

E

Y

F

A

M

K

I

Q

A

H

C

G

G

I

F

E

D

V

Y

P

K

V

M

T

S

K

V

E

E

K

K

P

A

Y

Y

S
|
S

I

T

D
|
D

E

S

N

N

L

M

W

L

S

G

R

A

S
x
T

I

H

E
|
E

G

A

G

K

K

D

L

L

E

E

-

Y

-

L

D

N

P

S

S

S

F

V

E

K

-

I

-

V

-

N

P

P

P

I

E

M

D

G

I

V

F

K

E

F

W

W

T

D

A

E

S

S

P

-

A

V

K

K

A

I

P

P

D

-

E

-

P

A

E

E

K

E

V

V

E

T

I

V

Y

R

F

F

E

E

Q

Q

G

G

V

H

P

P

V

V

A

A

I

L

N

N

G

G

E

K

R

T

L

F

P

S

G

D

-

D

L

V

E

E

L

M

I

M

R

L

K

E

L

A

N

N

E

R

I

I

G

G

R

R

H

H

G

G

V

L

G

G

R

M

T

S

D

D

M

Q

M

I

E
|
E

D

N

R

R

V

I

L

I

G

E

L

A

K

K

A
x
S

R

R

E

G

N

I

Y
|
Y

E

E

H

A

P

P

A

G

A

M

T

A

I

L

L

L

I

H

T

I

A

A

H

Y

R

E

D

R

L

L

E

L

K

T

L

G

V

I

L

H

S

N

R

E

R

D

E

T

L

I

K

E

F

Q

K

Y

K

H

F

A

V

H

E

G

E

R

E

Q

W

L

A

G

E

R

L

L

V

L

Y

Y

Y

Q

G

G

L

R

T

W

N

F

D

D

P

S

-

Q

-

A

-

L

-

M

L

L

F

R

D

D

A

S

L

L

N

Q

A

R

F

W

I

V

D

-

E

-

T

-

Q

A

K

S

R

Q

V

I

T

T

G

G

W

E

V

V

R

T

L

L

K

E

L

L

F

R

K

R

G

G

S

N

active site: D22 (= D12), R106 (= R91), D135 (= D120), S191 (= S169)
binding adenosine-5'-triphosphate: A16 (≠ S6), S18 (= S8), G20 (= G10), D22 (= D12), T23 (= T13), T41 (= T33), A42 (≠ V34), D127 (≠ H112), G128 (= G113), S129 (≠ C114), F139 (= F124), D193 (= D171)
binding citrulline: Y98 (= Y83), T102 (= T87), P103 (≠ S88), T130 (= T115), G133 (= G118), N134 (= N119), D135 (= D120), R138 (= R123), D193 (= D171), T200 (≠ S178), E202 (= E180), E202 (= E180), E279 (= E254), S287 (≠ A262), Y291 (= Y266)

P0A6E4 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Escherichia coli (strain K12) (see 4 papers)
28% identity, 87% coverage: 2:341/393 of query aligns to 13:368/447 of P0A6E4

query
sites
P0A6E4

K

R

V

I

V

G

L

I

S
x
A

Y
x
F

S
|
S

G
|
G

L
|
L

D
|
D

T
|
T

T
x
S

V

A

C

A

I

L

P

L

L

W

L

M

K

R

E

Q

K

K

Y

G

G

A

F

V

D

P

E

-

V

-

V

Y

T

A

V

Y

T

T

V
x
A

D

N

I

L

G

G

Q

Q

P

P

P

D

E

E

I

D

K

Y

E

D

A

A

-

I

E

P

K

R

R

R

G

A

K

M

K

E

Y

Y

-

G

A

A

D

E

K

N

H

A

Y

R

T

L

I

I

D

D

A

C

K

R

E

K

E

Q

F

L

V

V

R

A

L

E

L

G

F

I

R

A

L

A

I

I

K

Q

A

C

N

G

G

A

D

F

Y

H

E

N

G

T

-

T

-

G

-

L

-

T

Y
|
Y

V

F

L

N

G

T

T

T

S

P

L

L

A

G

R

R

P

A

L

V

I

T

A

G

E

T

K

M

I

L

V

V

E

A

V

A

A

M

K

K

K

E

E

D

N

G

A

V

D

N

A

I

I

W

A

G

H

D

G
|
G

C

S

T
|
T

G

Y

K

K

G

G

N
|
N

D
|
D

Q

I

L

E

R
|
R

F

F

E

-

N

-

V

-

F

-

Y

Y

Q

R

Y

Y

G

G

F

L

R

L

V

T

I

N

A

A

P

-

M

-

R

-

E

E

L

L

N

Q

L

I

T

Y

R

K

E

P

W

W

-

L

-

D

-

T

-

D

-

F

-

I

-

D

-

E

-

L

-

G

-

R

-

H

-

E

E

M

I

S

E

E

Y

F

A

M

K

I

Q

A

H

C

G

G

I

F

E

D

V

Y

P

K

V

M

T

S

K

V

E

E

K

K

P

A

Y

Y

S
|
S

I

T

D
|
D

E

S

N

N

L

M

W

L

S

G

R

A

S
x
T

I

H

E
|
E

G

A

G

K

K

D

L

L

E

E

-

Y

-

L

D

N

P

S

S

S

F

V

E

K

-

I

-

V

-

N

P

P

P

I

E

M

D

G

I

V

F

K

E

F

W

W

T

D

A

E

S

S

P

-

A

V

K

K

A

I

P

P

D

-

E

-

P

A

E

E

K

E

V

V

E

T

I

V

Y

R

F

F

E

E

Q

Q

G

G

V

H

P

P

V

V

A

A

I

L

N

N

G

G

E

K

R

T

L

F

P

S

G

D

-

D

L

V

E

E

L

M

I

M

R

L

K

E

L

A

N

N

E

R

I

I

G

G

R

R

H

H

G

G

V

L

G

G

R

M

T

S

D

D

M

Q

M

I

E
|
E

D

N

R

R

V

I

L

I

G

E

L

A

K

K

A

S

R

R

E

G

N

I

Y

Y

E

E

H

A

P

P

A

G

A

M

T

A

I

L

L

L

I

H

T

I

A

A

H

Y

R

E

D

R

L

L

E

L

K

T

L

G

V

I

L

H

S

N

R

E

R

D

E

T

L

I

K

E

F

Q

K

Y

K

H

F

A

V

H

E

G

E

R

E

Q

W

L

A

G

E

R

L

L

V

L

Y

Y

Y

Q

G

G

L

R

T

W

N

F

D

D

P

S

-

Q

-

A

-

L

-

M

L

L

F

R

D

D

A

S

L

L

N

Q

A

R

F

W

I

V

D

-

E

-

T

-

Q

A

K

S

R

Q

V

I

T

T

G

G

W

E

V

V

R

T

L

L

K

E

L

L

F

R

K

R

G

G

S

N

17:25 (vs. 6:14, 67% identical) binding ATP
A43 (≠ V34) binding ATP
Y99 (= Y83) binding L-citrulline
G129 (= G113) binding ATP
T131 (= T115) binding ATP; binding L-aspartate
N135 (= N119) binding L-aspartate; binding L-citrulline
D136 (= D120) binding ATP; binding L-aspartate
R139 (= R123) binding L-citrulline
S192 (= S169) binding L-citrulline
D194 (= D171) binding ATP
T201 (≠ S178) binding L-citrulline
E203 (= E180) binding L-citrulline
E280 (= E254) binding L-citrulline

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_013684503.1 NCBI__GCF_000194625.1:WP_013684503.1
MKVVLSYSGGLDTTVCIPLLKEKYGFDEVVTVTVDIGQPPEEIKEAEKRGKKYADKHYTI
DAKEEFVRLLFRLIKANGDYEGYVLGTSLARPLIAEKIVEVAKKENADAIAHGCTGKGND
QLRFENVFYQYGFRVIAPMRELNLTREWEIEYAKQHGIEVPVTKEKPYSIDENLWSRSIE
GGKLEDPSFEPPEDIFEWTASPAKAPDEPEKVEIYFEQGVPVAINGERLPGLELIRKLNE
IGGRHGVGRTDMMEDRVLGLKARENYEHPAATILITAHRDLEKLVLSRRELKFKKFVEEE
WAELVYYGLTNDPLFDALNAFIDETQKRVTGWVRLKLFKGSVTPVARYSEFALYREELVS
FDTTAIDQKYAEGFSRFHGLQGRIYREIIKKKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory