SitesBLAST
Comparing WP_013684515.1 NCBI__GCF_000194625.1:WP_013684515.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
39% identity, 42% coverage: 16:194/431 of query aligns to 3:187/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G25), Q15 (≠ R28), G16 (= G29), I17 (= I30), D36 (≠ E49), V63 (= V75), N89 (= N101), A91 (≠ T103), S94 (vs. gap), I142 (= I151), S143 (≠ T152), S144 (= S153), Y157 (= Y163), K161 (= K167), P187 (= P194)
Sites not aligning to the query:
4nbuB Crystal structure of fabg from bacillus sp (see paper)
38% identity, 43% coverage: 14:199/431 of query aligns to 3:187/244 of 4nbuB
- active site: G18 (= G29), N111 (= N125), S139 (≠ T150), Q149 (≠ V160), Y152 (= Y163), K156 (= K167)
- binding acetoacetyl-coenzyme a: D93 (≠ T107), K98 (≠ E112), S139 (≠ T150), N146 (≠ M157), V147 (≠ P158), Q149 (≠ V160), Y152 (= Y163), F184 (≠ M196)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G25), N17 (≠ R28), G18 (= G29), I19 (= I30), D38 (≠ E49), F39 (≠ I50), V59 (≠ T73), D60 (= D74), V61 (= V75), N87 (= N101), A88 (= A102), G89 (≠ T103), I90 (≠ V104), T137 (vs. gap), S139 (≠ T150), Y152 (= Y163), K156 (= K167), P182 (= P194), F184 (≠ M196), T185 (≠ V197), T187 (= T199)
Sites not aligning to the query:
8g9vE Crystal structures of 17-beta-hydroxysteroid dehydrogenase 13 (see paper)
33% identity, 46% coverage: 15:211/431 of query aligns to 33:235/293 of 8g9vE
- binding nicotinamide-adenine-dinucleotide: G43 (= G25), H46 (≠ R28), G47 (= G29), I48 (= I30), D67 (≠ E49), I68 (= I50), D93 (= D74), C94 (≠ V75), N120 (= N101), A121 (= A102), G122 (≠ T103), V170 (≠ I151), S172 (= S153), Y185 (= Y163), K189 (= K167), P218 (= P194), F220 (≠ M196), V221 (= V197), T223 (= T199), F225 (≠ G201)
- binding 4-{[2,5-dimethyl-3-(4-methylbenzene-1-sulfonyl)benzene-1-sulfonyl]amino}benzoic acid: S172 (= S153), V173 (vs. gap), C174 (vs. gap), I179 (≠ M157), Y185 (= Y163), V219 (≠ G195), F220 (≠ M196), F225 (≠ G201), W234 (≠ A210)
Sites not aligning to the query:
Q7Z5P4 17-beta-hydroxysteroid dehydrogenase 13; 17-beta-HSD 13; Hepatic retinol/retinal dehydrogenase; Short chain dehydrogenase/reductase family 16C member 3; Short-chain dehydrogenase/reductase 9; EC 1.1.1.-; EC 1.1.1.62; EC 1.1.1.105 from Homo sapiens (Human) (see 2 papers)
34% identity, 46% coverage: 15:211/431 of query aligns to 33:235/300 of Q7Z5P4
- GIG 47:49 (= GIG 29:31) mutation to AIA: Loss of retinol/retinal dehydrogenase activity.
- 69:84 (vs. 51:65, 31% identical) mutation Missing: Does not localize to lipid droplets.
- 85:93 (vs. 66:74, 22% identical) mutation Missing: Does not localize to lipid droplets.
- 94:106 (vs. 75:87, 31% identical) mutation Missing: Does not localize to lipid droplets.
- R97 (≠ E78) mutation to A: Decreased retinol/retinal dehydrogenase activity; when associated with A-101.
- Y101 (≠ K82) mutation to A: Decreased retinol/retinal dehydrogenase activity; when associated with A-97.
- N144 (= N125) mutation to A: Loss of retinol/retinal dehydrogenase activity.
- K153 (= K134) mutation to A: Loss of retinol/retinal dehydrogenase activity; when associated with A-156.
- L156 (= L137) mutation to A: Loss of retinol/retinal dehydrogenase activity; when associated with A-153.
- S172 (= S153) mutation to A: Loss of retinol/retinal dehydrogenase activity.
- Y185 (= Y163) mutation to A: Loss of retinol/retinal dehydrogenase activity; when associated with A-189.
- K189 (= K167) mutation to A: Loss of retinol/retinal dehydrogenase activity; when associated with A-185.
- L199 (= L177) mutation to A: Loss of retinol/retinal dehydrogenase activity; when associated with A-202.
- E202 (= E180) mutation to A: Loss of retinol/retinal dehydrogenase activity; when associated with A-199.
- K208 (≠ E184) mutation to A: Decreased retinol/retinal dehydrogenase activity.
Sites not aligning to the query:
- 22:28 mutation Missing: Does not localize to lipid droplets.
- 260 P → S: loss of retinol/retinal dehydrogenase activity; does not affect localization to lipid droplets; dbSNP:rs62305723
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
34% identity, 44% coverage: 16:203/431 of query aligns to 6:194/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G25), R18 (= R28), G19 (= G29), I20 (= I30), D39 (≠ E49), R40 (≠ I50), C63 (≠ T73), I65 (≠ V75), N91 (= N101), G93 (≠ T103), I94 (≠ V104), V114 (≠ I124), Y155 (= Y163), K159 (= K167), I188 (≠ V197), T190 (= T199), T193 (≠ I202)
2ewmB Crystal structure of the (s)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain ebn1 (see paper)
36% identity, 43% coverage: 16:199/431 of query aligns to 3:187/247 of 2ewmB
- active site: G16 (= G29), S139 (= S153), Y149 (= Y163), Y152 (vs. gap), K156 (= K167)
- binding nicotinamide-adenine-dinucleotide: G12 (= G25), N15 (≠ R28), G16 (= G29), I17 (= I30), D36 (≠ E49), L37 (≠ I50), C59 (≠ T73), D60 (= D74), V61 (= V75), N87 (= N101), S139 (= S153), Y152 (vs. gap), K156 (= K167), P182 (= P194), S183 (≠ G195), L184 (≠ M196), V185 (= V197)
Sites not aligning to the query:
3pgxA Crystal structure of a putative carveol dehydrogenase from mycobacterium paratuberculosis bound to nicotinamide adenine dinucleotide (see paper)
33% identity, 59% coverage: 15:268/431 of query aligns to 2:268/270 of 3pgxA
- active site: G16 (= G29), S155 (= S153), Y168 (= Y163), K172 (= K167)
- binding nicotinamide-adenine-dinucleotide: G12 (= G25), R15 (= R28), G16 (= G29), Q17 (≠ I30), D36 (≠ E49), I37 (= I50), A50 (vs. gap), D75 (= D74), V76 (= V75), N102 (= N101), A103 (= A102), G104 (≠ T103), V105 (= V104), V125 (≠ I124), V153 (≠ I151), Y168 (= Y163), K172 (= K167), P198 (= P194), V201 (= V197), T203 (= T199), M205 (≠ G201), I206 (= I202)
Q5P5I4 (S)-1-Phenylethanol dehydrogenase; EC 1.1.1.311 from Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) (see 2 papers)
36% identity, 43% coverage: 16:199/431 of query aligns to 5:189/249 of Q5P5I4
- NGI 17:19 (≠ RGI 28:30) binding NAD(+)
- D38 (≠ E49) binding NAD(+)
- CDV 61:63 (≠ TDV 73:75) binding NAD(+)
- N89 (= N101) binding NAD(+)
- Y93 (≠ V105) binding NAD(+)
- K158 (= K167) binding NAD(+)
- PSLV 184:187 (≠ PGMV 194:197) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 191 binding NAD(+)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
34% identity, 42% coverage: 19:200/431 of query aligns to 6:189/248 of Q9KJF1
- S15 (≠ R28) binding NAD(+)
- D36 (≠ E49) binding NAD(+)
- D62 (= D74) binding NAD(+)
- I63 (≠ V75) binding NAD(+)
- N89 (= N101) binding NAD(+)
- Y153 (= Y163) binding NAD(+)
- K157 (= K167) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Q73SC8 Uncharacterized NAD-dependent oxidoreductase MAP_4146; EC 1.-.-.- from Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) (Mycobacterium paratuberculosis)
33% identity, 59% coverage: 15:268/431 of query aligns to 7:273/275 of Q73SC8
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
34% identity, 42% coverage: 19:200/431 of query aligns to 5:188/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G25), M16 (≠ I30), D35 (≠ E49), I36 (= I50), I62 (≠ V75), N88 (= N101), G90 (≠ T103), I138 (= I151), S140 (= S153), Y152 (= Y163), K156 (= K167), I185 (≠ V197)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
36% identity, 43% coverage: 14:199/431 of query aligns to 2:191/247 of P73574
- A14 (= A26) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P158) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K167) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ M196) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
Sites not aligning to the query:
- 198 N→R: 3.5-fold increase in activity on acetoacetyl-CoA.
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 42% coverage: 18:199/431 of query aligns to 5:186/244 of P0AEK2
- GASR 12:15 (≠ GAGR 25:28) binding NADP(+)
- T37 (≠ I50) binding NADP(+)
- NV 59:60 (≠ DV 74:75) binding NADP(+)
- N86 (= N101) binding NADP(+)
- Y151 (= Y163) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSASK 163:167) binding NADP(+)
- A154 (≠ S166) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K167) mutation to A: Defect in the affinity for NADPH.
- I184 (≠ V197) binding NADP(+)
Sites not aligning to the query:
- 233 E→K: Decreases in the thermolability of the reductase; when associated with T-154.
D4A1J4 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Rattus norvegicus (Rat) (see paper)
37% identity, 44% coverage: 16:203/431 of query aligns to 4:186/245 of D4A1J4
- Y147 (= Y163) mutation to F: Loss of function.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
36% identity, 42% coverage: 18:199/431 of query aligns to 4:185/243 of 1q7bA
- active site: G15 (= G29), E101 (= E117), S137 (= S153), Q147 (≠ V160), Y150 (= Y163), K154 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G25), S13 (≠ G27), R14 (= R28), T36 (≠ I50), N58 (≠ D74), V59 (= V75), N85 (= N101), A86 (= A102), G87 (≠ T103), I88 (≠ V104), S137 (= S153), Y150 (= Y163), K154 (= K167), P180 (= P194), G181 (= G195), I183 (≠ V197)
Sites not aligning to the query:
8dt1C Crystal structure of a putative d-beta-hydroxybutyrate dehydrogenase from burkholderia cenocepacia j2315 in complex with NAD
38% identity, 48% coverage: 16:223/431 of query aligns to 2:213/259 of 8dt1C
- binding nicotinamide-adenine-dinucleotide: G11 (= G25), S14 (≠ R28), G15 (= G29), I16 (= I30), D35 (≠ E49), L36 (≠ I50), M60 (≠ T73), V62 (= V75), N88 (= N101), A89 (= A102), M139 (vs. gap), Y154 (= Y163), K158 (= K167), P184 (= P194), G185 (= G195), F186 (≠ M196), V187 (= V197), T189 (= T199)
6zzpA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and 3-oxovalerate (see paper)
33% identity, 48% coverage: 16:222/431 of query aligns to 8:218/265 of 6zzpA
- binding nicotinamide-adenine-dinucleotide: G17 (= G25), S20 (≠ R28), G21 (= G29), I22 (= I30), D41 (≠ E49), I42 (= I50), M66 (≠ T73), D67 (= D74), V68 (= V75), N94 (= N101), A95 (= A102), G96 (≠ T103), M145 (vs. gap), S147 (≠ T150), Y160 (= Y163), K164 (= K167), P190 (= P194), F192 (≠ M196), V193 (= V197), T195 (= T199), L197 (≠ G201), V198 (≠ I202)
- binding 3-oxidanylidenepentanoic acid: Q98 (≠ V105), S147 (≠ T150), H149 (≠ T152), K157 (≠ V160), Y160 (= Y163), F192 (≠ M196), Q201 (≠ A205)
6zzoC Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and acetoacetate (see paper)
33% identity, 48% coverage: 16:222/431 of query aligns to 8:218/265 of 6zzoC
- binding acetoacetic acid: Q98 (≠ V105), H149 (≠ T152), K157 (≠ V160), F192 (≠ M196), Q201 (≠ A205)
- binding nicotinamide-adenine-dinucleotide: G17 (= G25), S20 (≠ R28), G21 (= G29), I22 (= I30), D41 (≠ E49), I42 (= I50), M66 (≠ T73), D67 (= D74), V68 (= V75), N94 (= N101), A95 (= A102), G96 (≠ T103), M145 (vs. gap), Y160 (= Y163), K164 (= K167), P190 (= P194), F192 (≠ M196), V193 (= V197), T195 (= T199), L197 (≠ G201), V198 (≠ I202)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
36% identity, 42% coverage: 18:199/431 of query aligns to 4:185/243 of 1q7cA
- active site: G15 (= G29), S137 (= S153), Q147 (≠ V160), F150 (≠ Y163), K154 (= K167)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G25), S13 (≠ G27), R14 (= R28), A35 (≠ E49), T36 (≠ I50), L57 (≠ V71), N58 (≠ D74), V59 (= V75), G87 (≠ T103), I88 (≠ V104)
1g0oC Structure of trihydroxynaphthalene reductase in complex with NADPH and pyroquilon (see paper)
34% identity, 47% coverage: 9:211/431 of query aligns to 18:223/281 of 1g0oC
- active site: G38 (= G29), S162 (≠ T150), H173 (≠ V160), Y176 (= Y163), K180 (= K167), Y221 (≠ L209)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G34 (= G25), R37 (= R28), G38 (= G29), I39 (= I30), A59 (= A48), N60 (≠ E49), S61 (≠ I50), N85 (≠ D74), V86 (= V75), N112 (= N101), S113 (≠ A102), G114 (≠ T103), M160 (vs. gap), Y176 (= Y163), K180 (= K167), P206 (= P194), G208 (≠ M196), I209 (≠ V197), T211 (= T199), M213 (≠ G201)
- binding pyroquilon: S162 (≠ T150), I163 (= I151), Y176 (= Y163), G208 (≠ M196), Y221 (≠ L209)
Query Sequence
>WP_013684515.1 NCBI__GCF_000194625.1:WP_013684515.1
MLVEKMEKMGLSPSALAGKVAVITGAGRGIGKELARALAWLGAKVVIAEIRDTGAEVEAL
IRSEGGMALFVRTDVGDEESIKRLANKVFEEFGKVDILINNATVVKTGSILELPLEEWDQ
TWSINVRAAILTIKAFLPGMLEREEGVIVTITSDEGMPYVAPYSASKAVLSSLGLSLAAE
LGNESGISVFVFAPGMVDTPGIRNAARELAPRYGMTYEEFIDQSVNPGYDGLMPAEDCAA
GFAYCIVNAQDYHGQIADPFQPLAKAGLLSFSSQQKSEDGSVDLSKTSEKVSRSESAVEL
ARELKKILEDVNRETNELDMFRKMWVTRTFRKRCGMGIKDWLETITELISELEEVDQAIE
TGDTAKAEGIRGNLTWMKSNLEKLADYFKKNMKDAEGYFKDPKALSAALDVLAYRENTVR
LLILELEQILE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory