SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013705639.1 NCBI__GCF_000195295.1:WP_013705639.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
45% identity, 93% coverage: 10:226/233 of query aligns to 1:221/232 of 1f3oA

query
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1f3oA

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binding adenosine-5'-diphosphate: Y11 (= Y20), G41 (= G50), G43 (= G52), K44 (= K53), S45 (≠ T54), T46 (= T55)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
45% identity, 93% coverage: 10:226/233 of query aligns to 1:221/230 of 1l2tA

query
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binding adenosine-5'-triphosphate: Y11 (= Y20), S40 (= S49), G41 (= G50), S42 (= S51), G43 (= G52), K44 (= K53), S45 (≠ T54), T46 (= T55), F138 (vs. gap), Q145 (= Q150), S147 (= S152), G149 (= G154), Q150 (= Q155), H204 (= H209)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
46% identity, 94% coverage: 10:228/233 of query aligns to 3:220/226 of 5xu1B

query
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binding magnesium ion: G45 (= G52), K46 (= K53), S47 (≠ T54)

8g4cB Bceabs atpgs high res tm (see paper)
46% identity, 94% coverage: 9:226/233 of query aligns to 2:219/248 of 8g4cB

query
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8g4cB

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binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y20), S42 (= S49), S44 (= S51), T47 (= T54), D168 (= D175)

7tchB Bceab e169q variant atp-bound conformation (see paper)
45% identity, 94% coverage: 9:226/233 of query aligns to 1:218/245 of 7tchB

query
sites
7tchB

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binding adenosine-5'-triphosphate: Y12 (= Y20), Q19 (≠ I27), V21 (= V29), S41 (= S49), G42 (= G50), S43 (= S51), K45 (= K53), T46 (= T54), E142 (≠ Q150), G145 (= G153), G146 (= G154)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
44% identity, 93% coverage: 10:226/233 of query aligns to 4:219/648 of P75831

query
sites
P75831

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S

A

D

G

L

L

P

E

K

R

A

K

K

Q

R

R

R

L

Q

L

H

R

A

A

E

Q

L

E

A

L

L

L

K

Q

L

R

V

L

G

G

I

L

Y

E

D

D

R

R

R

T

D

E

H

Y

Y

Y

P

P

R

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

I

L

V

M

T

N

D

G

P

G

T

Q

I

V

I

I

A

L

A

A

D
|
D

E

E

P

P

T

T

G

G

D

A

L

L

D

D

K

S

V

H

S

S

A

G

E

E

E

E

V

V

L

M

N

A

L

I

M

L

T

H

R

Q

L

L

N

-

Q

R

E

D

L

R

R

G

K

H

T

T

I

V

I

I

M

I

V

V

T

T

H

H

D

D

P

P

R

Q

A

V

A

A

E

A

K

Q

S

A

T

E

R

R

L

V

Q

I

H

E

L

I

D

R

K

D

G

G

K47 (= K53) mutation to L: Lack of activity.
D169 (= D175) mutation to N: Lack of activity.

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
42% identity, 93% coverage: 10:226/233 of query aligns to 3:218/615 of 5lilA

query
sites
5lilA

I

I

V

I

E

E

I

I

K

K

D

Q

L

L

S

N

K

R

S

Y

Y
x
F

R

G

R

E

G

G

A

E

Q

N

I

R

I

V

P

H

V
|
V

L

L

Q

K

H

D

V

I

C

S

L

L

D

S

I

I

Q

E

E

R

G

G

E

D

F

F

L

V

A

A

L

I

M

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

M

N

N

L

I

I

I

A

G

G

C

I

L

D

D

Q

T

P

A

D

T

A

G

G

G

I

S

L

S

R

K

V

I

A

D

G

G

V

K

D

E

I

T

S

I

R

E

L

L

S

T

E

N

S

D

E

Q

L

L

A

S

V

D

W

L

R

R

H

S

R

Q

N

K

V

F

G

G

F

F

I

I

F

F

Q
|
Q

F

R

Y

Y

N

N

L

L

V

L

P

S

V

S

L

L

T

T

A

A

L

A

E

E

N

N

V

V

E

A

L

L

P

P

L

A

M

I

L

Y

S

A

D

G

L

M

P

P

K

Q

A

S

K

Q

R

R

R

L

Q

E

H

R

A

A

E

K

L

Q

A

L

L

L

K

E

L

K

V

L

G

G

I

L

Y

G

D

D

R
x
K

R

W

D

Q

H

N

Y

K

P

P

R

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

I

L

V

M

T

N

D

G

P

G

T

E

I

I

I

I

A

L

A

A

D

D

E

Q

P

P

T

T

G

G

D

A

L

L

D

D

K

S

V

H

S

S

A

G

E

E

E

N

V

V

L

M

N

E

L

I

M

L

T

R

R

Q

L

L

N

H

Q

E

E

E

L

-

R

G

K

H

T

T

I

I

I

I

M

M

V

V

T

T

H

H

D

D

P

K

R

H

A

I

A

A

E

A

K

S

S

A

T

N

R

R

L

I

Q

I

H

E

L

I

D

K

K

D

G

G

binding adenosine-5'-triphosphate: F13 (≠ Y20), V22 (= V29), S42 (= S49), G43 (= G50), G45 (= G52), K46 (= K53), S47 (≠ T54), T48 (= T55), Q92 (= Q99), K136 (≠ R143), Q143 (= Q150), S145 (= S152), G147 (= G154), Q148 (= Q155)
binding magnesium ion: S47 (≠ T54), Q92 (= Q99)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
42% identity, 93% coverage: 10:226/233 of query aligns to 3:218/592 of 5lj7A

query
sites
5lj7A

I

I

V

I

E

E

I

I

K

K

D

Q

L

L

S

N

K

R

S

Y

Y

F

R

G

R

E

G

G

A

E

Q

N

I

R

I

V

P

H

V
|
V

L

L

Q

K

H

D

V

I

C

S

L

L

D

S

I

I

Q

E

E

R

G

G

E

D

F

F

L

V

A

A

L

I

M

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

M

N

N

L

I

I

I

A

G

G

C

I

L

D

D

Q

T

P

A

D

T

A

G

G

G

I

S

L

S

R

K

V

I

A

D

G

G

V

K

D

E

I

T

S

I

R

E

L

L

S

T

E

N

S

D

E

Q

L

L

A

S

V

D

W

L

R

R

H

S

R

Q

N

K

V

F

G

G

F

F

I

I

F

F

Q
|
Q

F

R

Y

Y

N

N

L

L

V

L

P

S

V

S

L

L

T

T

A

A

L

A

E

E

N

N

V

V

E

A

L

L

P

P

L

A

M

I

L

Y

S

A

D

G

L

M

P

P

K

Q

A

S

K

Q

R

R

R

L

Q

E

H

R

A

A

E

K

L

Q

A

L

L

L

K

E

L

K

V

L

G

G

I

L

Y

G

D

D

R
x
K

R

W

D

Q

H

N

Y

K

P

P

R

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

I

L

V

M

T

N

D

G

P

G

T

E

I

I

I

I

A

L

A

A

D

D

E

Q

P

P

T

T

G

G

D

A

L

L

D

D

K

S

V

H

S

S

A

G

E

E

E

N

V

V

L

M

N

E

L

I

M

L

T

R

R

Q

L

L

N

H

Q

E

E

E

L

-

R

G

K

H

T

T

I

I

I

I

M

M

V

V

T

T

H

H

D

D

P

K

R

H

A

I

A

A

E

A

K

S

S

A

T

N

R

R

L

I

Q

I

H

E

L

I

D

K

K

D

G

G

binding adenosine-5'-triphosphate: V22 (= V29), S42 (= S49), G43 (= G50), G45 (= G52), K46 (= K53), S47 (≠ T54), T48 (= T55), Q92 (= Q99), K136 (≠ R143), Q143 (= Q150), S145 (= S152), G147 (= G154), Q148 (= Q155)
binding magnesium ion: S47 (≠ T54), Q92 (= Q99)

7mdyC Lolcde nucleotide-bound
40% identity, 94% coverage: 9:228/233 of query aligns to 1:220/226 of 7mdyC

query
sites
7mdyC

V

I

I

L

V

L

E

Q

I

C

K

D

D

N

L

L

S

C

K

K

S

R

Y
|
Y

R

Q

R

E

G

G

A

S

Q

V

I

Q

I

T

P

D

V

V

L

L

Q

H

H

N

V

V

C

S

L

F

D

S

I

V

Q

G

E

E

G

G

E

E

F

M

L

M

A

A

L

I

M

V

G

G

P

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

N

H

L

L

I

L

A

G

G

G

I

L

D

D

Q

T

P

P

D

T

A

S

G

G

I

D

L

V

R

I

V

F

A

N

G

G

V

Q

D

P

I

M

S

S

R

K

L

L

S

S

E

S

S

A

E

A

L

K

A

A

V

E

W

L

R

R

H

N

R

Q

N

K

V

L

G

G

F

F

I

I

F

Y

Q
|
Q

F

F

Y

H

N

H

L

L

V

L

P

P

V

D

L

F

T

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

M

L

L

I

S

G

D

K

L

K

P

K

K

P

A

A

K

E

R

I

R

N

Q

S

H

R

A

A

E

L

L

E

A

M

L

L

K

K

L

A

V

V

G

G

I

L

Y

D

D

H

R

R

R

A

D

N

H
|
H

Y

R

P

P

R

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

T

N

D

N

P

P

T

R

I

L

I

V

A

L

A

A

D

D

E
|
E

P

P

T

T

G

G

D
x
N

L

L

D

D

K

A

V

R

S

N

A

A

E

D

E

S

V

I

L

F

N

Q

L

L

M

L

T

G

R

E

L

L

N

N

Q

R

E

L

L

Q

R

G

K

T

T

A

I

F

I

L

M

V

V

V

T

T

H
|
H

D

D

P

L

R

Q

A

L

A

A

E

K

K

R

S

M

T

S

R

R

L

Q

Q

L

H

E

L

M

D

R

K

D

G

G

V

R

L

L

binding adp orthovanadate: Y12 (= Y20), G42 (= G50), S43 (= S51), G44 (= G52), K45 (= K53), S46 (≠ T54), T47 (= T55), Q91 (= Q99), H138 (= H146), E142 (≠ Q150), S144 (= S152), G145 (= G153), G146 (= G154), E168 (= E176), N172 (≠ D180), H201 (= H209)
binding magnesium ion: S46 (≠ T54), Q91 (= Q99)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
41% identity, 91% coverage: 9:219/233 of query aligns to 4:214/233 of P75957

query
sites
P75957

V

I

I

L

V

L

E

Q

I

C

K

D

D

N

L

L

S

C

K

K

S

R

Y

Y

R

Q

R

E

G

G

A

S

Q

V

I

Q

I

T

P

D

V

V

L

L

Q

H

H

N

V

V

C

S

L

F

D

S

I

V

Q

G

E

E

G

G

E

E

F

M

L

M

A

A

L

I

M

V

G
|
G

P

S

S

S

G

G

S

S

G

G

K

K

T

S

T

T

L

L

L

L

N

H

L

L

I

L

A

G

G

G

I

L

D

D

Q

T

P

P

D

T

A

S

G

G

I

D

L

V

R

I

V

F

A

N

G

G

V

Q

D

P

I

M

S

S

R

K

L

L

S

S

E

S

S

A

E

A

L

K

A

A

V

E

W

L

R

R

H

N

R

Q

N

K

V

L

G

G

F

F

I

I

F

Y

Q

Q

F

F

Y

H

N

H

L

L

V

L

P

P

V

D

L

F

T

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

M

L

L

I

S

G

D

K

L

K

P

K

K

P

A

A

K

E

R

I

R

N

Q

S

H

R

A

A

E

L

L

E

A

M

L

L

K

K

L

A

V

V

G

G

I

L

Y

D

D

H

R

R

R

A

D

N

H

H

Y

R

P

P

R

S

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

T

N

D

N

P

P

T

R

I

L

I

V

A

L

A

A

D

D

E

E

P

P

T

T

G

G

D

N

L

L

D

D

K

A

V

R

S

N

A

A

E

D

E

S

V

I

L

F

N

Q

L

L

M

L

T

G

R

E

L

L

N

N

Q

R

E

L

L

Q

R

G

K

T

T

A

I

F

I

L

M

V

V

V

T

T

H

H

D

D

P

L

R

Q

A

L

A

A

E

K

K

R

S

M

T

S

R

R

G42 (= G47) mutation to D: Loss of lipoprotein release when overexpressed.

7arlD Lolcde in complex with lipoprotein and adp (see paper)
40% identity, 94% coverage: 9:228/233 of query aligns to 1:220/222 of 7arlD

query
sites
7arlD

V

I

I

L

V

L

E

Q

I

C

K

D

D

N

L

L

S

C

K

K

S

R

Y

Y

R

Q

R

E

G

G

A

S

Q

V

I

Q

I

T

P

D

V

V

L

L

Q

H

H

N

V

V

C

S

L

F

D

S

I

V

Q

G

E

E

G

G

E

E

F

M

L

M

A

A

L

I

M

V

G

G

P

S

S

S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T

T

L

L

L

L

N

H

L

L

I

L

A

G

G

G

I

L

D

D

Q

T

P

P

D

T

A

S

G

G

I

D

L

V

R

I

V

F

A

N

G

G

V

Q

D

P

I

M

S

S

R

K

L

L

S

S

E

S

S

A

E

A

L

K

A

A

V

E

W

L

R

R

H

N

R

Q

N

K

V

L

G

G

F

F

I

I

F

Y

Q

Q

F

F

Y

H

N

H

L

L

V

L

P

P

V

D

L

F

T

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

M

L

L

I

S

G

D

K

L

K

P

K

K

P

A

A

K

E

R

I

R

N

Q

S

H

R

A

A

E

L

L

E

A

M

L

L

K

K

L

A

V

V

G

G

I

L

Y

D

D

H

R

R

R

A

D

N

H

H

Y

R

P

P

R

S

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

T

N

D

N

P

P

T

R

I

L

I

V

A

L

A

A

D

D

E

E

P

P

T

T

G

G

D

N

L

L

D

D

K

A

V

R

S

N

A

A

E

D

E

S

V

I

L

F

N

Q

L

L

M

L

T

G

R

E

L

L

N

N

Q

R

E

L

L

Q

R

G

K

T

T

A

I

F

I

L

M

V

V

V

T

T

H

H

D

D

P

L

R

Q

A

L

A

A

E

K

K

R

S

M

T

S

R

R

L

Q

Q

L

H

E

L

M

D

R

K

D

G

G

V

R

L

L

binding adenosine-5'-diphosphate: G42 (= G50), G44 (= G52), K45 (= K53), S46 (≠ T54)

9gvkD Cryo-em structure of endogenous atp-bound lolcde with lold-e171q mutations in nanodiscs (see paper)
40% identity, 94% coverage: 9:228/233 of query aligns to 2:221/226 of 9gvkD

query
sites
9gvkD

V

I

I

L

V

L

E

Q

I

C

K

D

D

N

L

L

S

C

K

K

S

R

Y
|
Y

R

Q

R

E

G

G

A

S

Q

V

I

Q

I

T

P

D

V

V

L

L

Q

H

H

N

V

V

C

S

L

F

D

S

I

V

Q

G

E

E

G

G

E

E

F

M

L

M

A

A

L

I

M

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

N

H

L

L

I

L

A

G

G

G

I

L

D

D

Q

T

P

P

D

T

A

S

G

G

I

D

L

V

R

I

V

F

A

N

G

G

V

Q

D

P

I

M

S

S

R

K

L

L

S

S

E

S

S

A

E

A

L

K

A

A

V

E

W

L

R

R

H

N

R

Q

N

K

V

L

G

G

F

F

I

I

F

Y

Q
|
Q

F

F

Y

H

N

H

L

L

V

L

P

P

V

D

L

F

T

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

M

L

L

I

S

G

D

K

L

K

P

K

K

P

A

A

K

E

R

I

R

N

Q

S

H

R

A

A

E

L

L

E

A

M

L

L

K

K

L

A

V

V

G

G

I

L

Y

D

D

H

R
|
R

R

A

D

N

H
|
H

Y

R

P

P

R

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

T

N

D

N

P

P

T

R

I

L

I

V

A

L

A

A

D

D

E

Q

P

P

T

T

G

G

D

N

L

L

D

D

K

A

V

R

S

N

A

A

E

D

E

S

V

I

L

F

N

Q

L

L

M

L

T

G

R

E

L

L

N

N

Q

R

E

L

L

Q

R

G

K

T

T

A

I

F

I

L

M

V

V

V

T

T

H

H

D

D

P

L

R

Q

A

L

A

A

E

K

K

R

S

M

T

S

R

R

L

Q

Q

L

H

E

L

M

D

R

K

D

G

G

V

R

L

L

binding adenosine-5'-triphosphate: Y13 (= Y20), S42 (= S49), G43 (= G50), G45 (= G52), K46 (= K53), S47 (≠ T54), T48 (= T55), Q92 (= Q99), R136 (= R143), H139 (= H146), E143 (≠ Q150), S145 (= S152), G146 (= G153), G147 (= G154), E148 (≠ Q155)
binding magnesium ion: S47 (≠ T54), Q92 (= Q99)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
41% identity, 91% coverage: 9:219/233 of query aligns to 3:213/229 of 7v8iD

query
sites
7v8iD

V

I

I

L

V

L

E

Q

I

C

K

D

D

N

L

L

S

C

K

K

S

R

Y

Y

R

Q

R

E

G

G

A

S

Q

V

I

Q

I

T

P

D

V
|
V

L

L

Q

H

H

N

V

V

C

S

L

F

D

S

I

V

Q

G

E

E

G

G

E

E

F

M

L

M

A

A

L

I

M

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

N

H

L

L

I

L

A

G

G

G

I

L

D

D

Q

T

P

P

D

T

A

S

G

G

I

D

L

V

R

I

V

F

A

N

G

G

V

Q

D

P

I

M

S

S

R

K

L

L

S

S

E

S

S

A

E

A

L

K

A

A

V

E

W

L

R

R

H

N

R

Q

N

K

V

L

G

G

F

F

I

I

F

Y

Q
|
Q

F

F

Y

H

N

H

L

L

V

L

P

P

V

D

L

F

T

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

M

L

L

I

S

G

D

K

L

K

P

K

K

P

A

A

K

E

R

I

R

N

Q

S

H

R

A

A

E

L

L

E

A

M

L

L

K

K

L

A

V

V

G

G

I

L

Y

D

D

H

R
|
R

R

A

D

N

H
|
H

Y

R

P

P

R

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

T

N

D

N

P

P

T

R

I

L

I

V

A

L

A

A

D

D

E

Q

P

P

T

T

G

G

D

N

L

L

D

D

K

A

V

R

S

N

A

A

E

D

E

S

V

I

L

F

N

Q

L

L

M

L

T

G

R

E

L

L

N

N

Q

R

E

L

L

Q

R

G

K

T

T

A

I

F

I

L

M

V

V

V

T

T

H
|
H

D

D

P

L

R

Q

A

L

A

A

E

K

K

R

S

M

T

S

R

R

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V29), S43 (= S49), G44 (= G50), G46 (= G52), K47 (= K53), S48 (≠ T54), T49 (= T55), Q93 (= Q99), R137 (= R143), H140 (= H146), E144 (≠ Q150), S146 (= S152), G148 (= G154), E149 (≠ Q155), H203 (= H209)
binding magnesium ion: S48 (≠ T54), Q93 (= Q99)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
40% identity, 88% coverage: 22:226/233 of query aligns to 14:214/223 of 2pclA

query
sites
2pclA

R

R

G

G

A

Y

Q

E

I

I

I

-

P

-

V

-

L

L

Q

K

H

G

V

I

C

S

L

L

D

S

I

V

Q

K

E

K

G

G

E

E

F

F

L

V

A

S

L

I

M

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

T

S

T

T

L

L

L

L

N

Y

L

I

I

L

A

G

G

L

I

L

D

D

Q

A

P

P

D

T

A

E

G

G

I

K

L

V

R

F

V

L

A

E

G

G

V

K

D

E

I

V

S

D

R

Y

L

T

S

N

E

E

S

K

E

E

L

L

A

S

V

L

W

L

R

R

H

N

R

R

N

K

V

L

G

G

F

F

I

V

F

F

Q

Q

F

F

Y

H

N

Y

L

L

V

I

P

P

V

E

L

L

T

T

A

A

L

L

E

E

N

N

V

V

E

I

L

V

P

P

L

M

M

L

L

K

S

M

D

G

L

K

P

P

K

K

A

K

K

E

R

A

R

K

Q

E

H

R

A

G

E

E

L

Y

A

L

L

L

K

S

L

E

V

L

G

G

I

L

Y

G

D

D

R

K

R

L

D

S

H

R

Y

K

P

P

R

Y

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

T

N

D

E

P

P

T

I

I

L

I

L

A

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

D

N

L

L

D

D

K

S

V

A

S

N

A

T

E

K

E

R

V

V

L

M

N

D

L

I

M

F

T

L

R

K

L

I

N

N

Q

-

E

E

L

G

R

G

K

T

T

S

I

I

I

V

M

M

V

V

T
|
T

H

H

D

E

P

R

R

E

A

L

A

A

E

E

K

L

S

T

T

H

R

R

L

T

Q

L

H

E

L

M

D

K

K

D

G

G

binding magnesium ion: E165 (= E176), P166 (= P177), T167 (= T178), T196 (= T208)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
41% identity, 93% coverage: 10:226/233 of query aligns to 4:219/650 of 5ws4A

query
sites
5ws4A

I

L

V

L

E

E

I

V

K

S

D

N

L

L

S

V

K

R

S

E

Y

F

R

P

R

A

G

G

A

E

Q

S

I

T

I

I

P

Q

V

I

L

L

Q

K

H

G

V

I

C

D

L

L

D

T

I

I

Q

Y

E

E

G

G

E

E

F

L

L

V

A

A

L

I

M

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

T
x
S

T

T

L

L

L

M

N

N

L

I

I

L

A

G

G

C

I

L

D

D

Q

R

P

P

D

T

A

S

G

G

I

S

L

Y

R

K

V

V

A

N

G

G

V

Q

D

E

I

T

S

G

R

K

L

L

S

E

E

P

S

D

E

Q

L

L

A

A

V

Q

W

L

R

R

H

R

R

E

N

Y

V

F

G

G

F

F

I

I

F

F

Q

Q

F

R

Y

Y

N

H

L

L

V

L

P

G

V

D

L

L

T

S

A

A

L

E

E

G

N

N

V

V

E

E

L

V

P

P

L

A

M

V

L

Y

S

A

D

G

L

V

P

T

K

P

A

A

K

D

R

R

R

K

Q

Q

H

R

A

A

E

T

L

A

A

L

L

L

K

T

L

E

V

L

G

G

I

L

Y

G

D

T

R

K

R

T

D

Q

H

N

Y

R

P

P

R

S

Q

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

I

L

V

M

T

N

D

G

P

G

T

D

I

V

I

I

A

L

A

A

D

D

E

E

P

P

T

T

G

G

D

A

L

L

D

D

K

S

V

H

S

S

A

G

E

V

E

E

V

V

L

M

N

R

L

I

M

L

T

R

R

E

L

L

N

N

Q

A

E

A

L

-

R

G

K

H

T

T

I

I

I

I

M

L

V

V

T

T

H

H

D

D

P

M

R

Q

A

V

A

A

E

K

K

N

S

A

T

T

R

R

L

I

Q

I

H

E

L

I

D

S

K

D

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S49), G44 (= G50), S48 (≠ T54)

P9WQK5 Uncharacterized ABC transporter ATP-binding protein Rv0073 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 91% coverage: 11:223/233 of query aligns to 4:215/330 of P9WQK5

query
sites
P9WQK5

V

L

E

S

I

I

K

Q

D

N

L

L

S

V

K

V

S

E

Y

Y

R

Y

R

S

G

G

A

G

Q

Y

I

A

I

L

P

R

V

P

L

I

Q

N

H

G

V

L

C

N

L

L

D

D

I

V

Q

A

E

A

G

G

E

S

F

L

L

V

A

M

L

L

M

L

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

T

T

T

L

L

L

L

N

S

L

C

I

L

A

G

G

G

I

I

D

L

Q

R

P

P

D

K

A

S

G

G

I

A

L

I

R
x
K

V

F

A

D

G

E

V

V

D

D

I

I

S

T

R

T

L

L

S

Q

E

G

S

A

E

E

L

L

A

A

V

N

W

Y

R

R

H

R

R

N

N

K

V

V

G

G

F

I

I

V

F

F

Q

Q

F

A

Y

F

N

N

L

L

V

V

P

P

V

S

L

L

T

T

A

A

L

V

E

E

N

N

V

V

E

M

L

V

P

P

L

L

M

R

L

S

S

A

D

G

L

M

P

S

K

R

A

R

K

A

R

S

R

R

Q

R

H

R

A

A

E

E

L

E

A

L

L

L

K

A

L

R

V

V

G

N

I

L

Y

A

D

E

R

R

R

M

D

N

H

H

Y

R

P

P

R

G

Q

D

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

A

I

V

A

A

R

R

A

A

I

I

V

A

T

L

D

D

P

P

T

P

I

L

I

I

A

L

A

A

D

D

E

E

P

P

T

T

G

A

D

H

L

L

D

D

K

F

V

I

S

Q

A

V

E

E

E

E

V

V

L

L

N

R

L

L

M

I

T

R

R

E

L

L

N

-

Q

A

E

D

L

G

R

E

K

R

T

V

I

V

I

V

M

V

V

A

T

T

H

H

D

D

P

S

R

R

A

M

A

L

E

P

K

M

S

A

T

D

R

R

L

V

Q

V

H

E

L

L

K65 (≠ R72) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 86% coverage: 10:210/233 of query aligns to 1:200/343 of P30750

query
sites
P30750

I

M

V

I

E

K

I

L

K

S

D

N

L

I

S

T

K

K

S

V

Y

F

R

H

R

Q

G

G

A

T

Q

R

I

T

I

I

P

Q

V

A

L

L

Q

N

H

N

V

V

C

S

L

L

D

H

I

V

Q

P

E

A

G

G

E

Q

F

I

L

Y

A

G

L

V

M

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

N

R

L

C

I

V

A

N

G

L

I

L

D

E

Q

R

P

P

D

T

A

E

G

G

I

S

L

V

R

L

V

V

A

D

G

G

V

Q

D

E

I

L

S

T

R

T

L

L

S

S

E

E

S

S

E

E

L

L

A

T

V

K

W

A

R

R

H

-

R

R

N

Q

V

I

G

G

F

M

I

I

F

F

Q

Q

F

H

Y

F

N

N

L

L

V

L

P

S

V

S

L

R

T

T

A

V

L

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

M

E

L

L

S

D

D

N

L

T

P

P

K

K

A

D

K

E

R

V

R

K

Q

R

H

R

A

V

E

T

L

E

A

L

L

L

K

S

L

L

V

V

G

G

I

L

Y

G

D

D

R

K

R

H

D

D

H

S

Y

Y

P

P

R

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

T

S

D

N

P

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E

P

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A

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E

R

E

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E

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N

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E

40:46 (vs. 49:55, 71% identical) binding ATP
E166 (= E176) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
40% identity, 96% coverage: 10:233/233 of query aligns to 1:220/222 of P0A9R7

query
sites
P0A9R7

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-

I

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K

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x
C

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N

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A

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A

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A

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A

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E

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L

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N

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I

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N

-

L

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G

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G

T

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G

G

K41 (= K53) mutation to R: Does not bind ATP.
C49 (≠ A61) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
40% identity, 94% coverage: 10:228/233 of query aligns to 1:215/219 of 8w6iD

query
sites
8w6iD

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R

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R

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Q

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-

I

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-

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x
A

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G

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x
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T

L

L

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S

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L

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R

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Q

F

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H

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N

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G

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Y

L

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x
K

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I

Q
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Q

L

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S

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G

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G

Q
x
E

Q

Q

Q

Q

R

R

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A

G

I

I

A

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R

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A

A

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D

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E

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T

G

G

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N

L

L

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G

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R

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R

E

L

F

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N

Q

R

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-

L

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K

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I

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I

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M

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T

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H
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H

D

D

P

I

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N

-

L

A

I

A

S

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R

K

R

S

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R

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L

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D

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G

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L

L

binding phosphothiophosphoric acid-adenylate ester: Y11 (= Y20), S37 (= S49), G38 (= G50), A39 (≠ S51), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55), Q86 (= Q99), K130 (≠ R143), Q137 (= Q150), S139 (= S152), G140 (= G153), G141 (= G154), E142 (≠ Q155), H195 (= H209)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
37% identity, 86% coverage: 10:210/233 of query aligns to 2:201/344 of 3tuzC

query
sites
3tuzC

I

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L

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x
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R
x
Q

G

G

A

T

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A

L

L

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H

N

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x
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T

L

L

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R

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G

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T

L

L

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E

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W

A

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R

R

N

Q

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I

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G

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M

I

I

F

F

Q

Q

F

H

Y

F

N

N

L

L

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L

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T

A

V

L

F

E

G

N

N

V

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A

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P

L

L

M

E

L

L

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D

N

L

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P

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R

A

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L

E

A

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L

L

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V

G

G

I

L

Y

G

D

D

R

K

R

H

D

D

H

S

Y

Y

P

P

R

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

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A

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S

D

N

P

P

T

K

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I

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A

L

A

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A

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T

G

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A

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L

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R

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E

L

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N

Q

R

E

R

L

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K

L

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T

H

H

D

E

binding adenosine-5'-diphosphate: F12 (≠ Y20), Q14 (≠ R22), G42 (= G50), G44 (= G52), K45 (= K53), S46 (≠ T54), T47 (= T55)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

Query Sequence

>WP_013705639.1 NCBI__GCF_000195295.1:WP_013705639.1
MASEVGLPVIVEIKDLSKSYRRGAQIIPVLQHVCLDIQEGEFLALMGPSGSGKTTLLNLI
AGIDQPDAGILRVAGVDISRLSESELAVWRHRNVGFIFQFYNLVPVLTALENVELPLMLS
DLPKAKRRQHAELALKLVGIYDRRDHYPRQLSGGQQQRVAIARAIVTDPTIIAADEPTGD
LDKVSAEEVLNLMTRLNQELRKTIIMVTHDPRAAEKSTRLQHLDKGVLKPCTG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory