SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013705949.1 NCBI__GCF_000195295.1:WP_013705949.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
44% identity, 94% coverage: 6:132/135 of query aligns to 5:131/138 of 3oa4A

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active site: H7 (= H8), I43 (≠ V44), E54 (= E55), H78 (= H79), E128 (= E129)
binding zinc ion: H7 (= H8), E54 (= E55), H78 (= H79), E128 (= E129)

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
38% identity, 96% coverage: 6:134/135 of query aligns to 5:139/139 of 6wfhA

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active site: H7 (= H8), E43 (≠ V44), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding (3S,5R,9R,19E)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,19-tetrahydroxy-8,8,20-trimethyl-10,14-dioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphahenicos-19-en-21-oic acid 3,5-dioxide (non-preferred name): H7 (= H8), Q39 (= Q40), Q60 (≠ E55), A70 (= A65), K73 (= K68), W74 (≠ Y69), H83 (= H78), H84 (= H79), L107 (≠ I102), G114 (= G109), S115 (≠ A110), F122 (= F117), P125 (= P120), K126 (= K121), L132 (= L127), E134 (= E129)

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
38% identity, 96% coverage: 6:134/135 of query aligns to 5:139/141 of 6wf6A

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active site: H7 (= H8), E43 (≠ V44), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E129)

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
38% identity, 96% coverage: 6:134/135 of query aligns to 5:139/144 of 6xbqA

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active site: H7 (= H8), E43 (≠ V44), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding carboxymethyldethia coenzyme *a: Q39 (= Q40), Q60 (≠ E55), A70 (= A65), K73 (= K68), W74 (≠ Y69), H83 (= H78), H84 (= H79), L107 (≠ I102), F122 (= F117), P125 (= P120), K126 (= K121), L132 (= L127)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E129)

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
38% identity, 96% coverage: 6:134/135 of query aligns to 5:139/144 of 6wfiA

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active site: H7 (= H8), E43 (≠ V44), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H8), Q39 (= Q40), Q60 (≠ E55), A70 (= A65), K73 (= K68), W74 (≠ Y69), H83 (= H78), H84 (= H79), L107 (≠ I102), G114 (= G109), S115 (≠ A110), I120 (= I115), F122 (= F117), P125 (= P120), L132 (= L127), E134 (= E129)

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
38% identity, 96% coverage: 6:134/135 of query aligns to 5:139/144 of 6wf7A

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active site: H7 (= H8), E43 (≠ V44), Q60 (≠ E55), H84 (= H79), E134 (= E129)
binding (S)-Methylmalonyl-Coenzyme A: Q39 (= Q40), E43 (≠ V44), Q60 (≠ E55), A70 (= A65), W74 (≠ Y69), H83 (= H78), H84 (= H79), L107 (≠ I102), G114 (= G109), S115 (≠ A110), F122 (= F117), P125 (= P120), K126 (= K121), L132 (= L127), E134 (= E129)
binding methylmalonyl-coenzyme a: Q39 (= Q40), Q60 (≠ E55), A70 (= A65), W74 (≠ Y69), H83 (= H78), H84 (= H79), L107 (≠ I102), G114 (= G109), S115 (≠ A110), I120 (= I115), F122 (= F117), P125 (= P120), K126 (= K121), L132 (= L127), E134 (= E129)

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
38% identity, 96% coverage: 6:134/135 of query aligns to 5:139/140 of 6xbtA

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active site: H7 (= H8), E43 (≠ V44), A60 (≠ E55), H84 (= H79), E134 (= E129)
binding cobalt (ii) ion: H7 (= H8), H84 (= H79), E134 (= E129)
binding coenzyme a: Q39 (= Q40), A70 (= A65), K73 (= K68), W74 (≠ Y69), K77 (= K72), H83 (= H78), L107 (≠ I102), F122 (= F117), P125 (= P120), K126 (= K121), L132 (= L127)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H8), Q39 (= Q40), A70 (= A65), K73 (= K68), W74 (≠ Y69), H83 (= H78), H84 (= H79), L107 (≠ I102), G114 (= G109), S115 (≠ A110), P125 (= P120), K126 (= K121), L132 (= L127), E134 (= E129)

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
34% identity, 94% coverage: 6:132/135 of query aligns to 48:175/176 of Q96PE7

query
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H50 (= H8) binding Co(2+)
R104 (≠ P62) to L: in dbSNP:rs6748672
H122 (= H79) binding Co(2+)
E172 (= E129) binding Co(2+)

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
34% identity, 94% coverage: 6:132/135 of query aligns to 12:137/138 of 6qh4C

query
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active site: H14 (= H8), V50 (= V44), E61 (= E55), H86 (= H79), E134 (= E129)
binding cobalt (ii) ion: H14 (= H8), H86 (= H79), E134 (= E129)

2qh0A Crystal structure of a glyoxalase from clostridium acetobutylicum
31% identity, 93% coverage: 8:132/135 of query aligns to 6:128/129 of 2qh0A

query
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binding zinc ion: H6 (= H8), E53 (= E55), H76 (= H79), E125 (= E129)

Query Sequence

>WP_013705949.1 NCBI__GCF_000195295.1:WP_013705949.1
MKIKCISHLGIAVKELEPVKKLYGENLQLEGHHEEVVESQKVKVSFFKVGETNIELLLAT
SPESAVAKYIEKNGEGIHHVAFEVEDLESALEELKAAGVKLIDEKPREGAHGAKIAFIHP
KATHGVLIELCQHAH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory