SitesBLAST

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
35% identity, 98% coverage: 3:377/381 of query aligns to 9:390/401 of Q56YA5

query
sites
Q56YA5

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TGT 68:70 (= TGT 61:63) binding pyridoxal 5'-phosphate
T148 (= T139) binding pyridoxal 5'-phosphate
QK 200:201 (= QK 190:191) binding pyridoxal 5'-phosphate
K201 (= K191) binding 3-hydroxypyruvate
P251 (≠ S240) mutation to L: Abolishes aminotransferase activity.
R347 (= R334) binding 3-hydroxypyruvate

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
35% identity, 98% coverage: 3:377/381 of query aligns to 7:388/399 of 6pk1A

query
sites
6pk1A

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binding 3-hydroxypyruvic acid: F93 (= F88), R345 (= R334)
binding pyridoxal-5'-phosphate: T66 (= T61), G67 (= G62), T68 (= T63), W71 (≠ M66), F93 (= F88), T146 (= T139), D173 (= D165), V175 (≠ I167), K199 (= K191)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
35% identity, 94% coverage: 3:360/381 of query aligns to 16:375/381 of 2dr1A

query
sites
2dr1A

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binding pyridoxal-5'-phosphate: S75 (≠ T61), G76 (= G62), T77 (= T63), F102 (= F88), T153 (= T139), D178 (= D165), V180 (≠ I167), S201 (≠ G188), K204 (= K191)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
31% identity, 97% coverage: 6:376/381 of query aligns to 10:384/387 of 3islA

query
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3islA

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A

I

V

V

C

A

I

M

Q

V

A

H

H

G

E

E

T
|
T

S

S

T

T

G

G

V

R

N

I

H

H

P

P

V

L

K

K

E

A

L

I

A

G

E

E

L

A

T

C

K

R

S

T

L

-

P

E

G

D

T

A

L

L

L

F

L

I

V

V

D
|
D

A

A

I
x
V

S

A

A

T

L

I

G

G

A

G

F

C

E

E

L

V

P

K

M

V

D

D

A

E

W

W

G

K

I

I

D

D

I

A

M

A

V

I

A

G

G

G

S

T

Q
|
Q

K
|
K

A

C

M

L

M

S

L

V

P

P

P

S

G

G

L

M

A

A

F

P

A

I

C

T

L

Y

S

N

E

E

K

R

A

V

W

A

E

D

F

V

-

I

-

A

T

A

K

R

T

N

A

R

T

P

C

I

N

T

K

S

Y

N

Y

Y

F

F

N

D

F

L

S

S

K

Q

-

L

E

E

L

D

K

Y

N

W

I

S

Q

E

K

R

N

R

T

L

G

N

A

H

Y

H

T

T

S

E

A

A

V

T

S

T

L

M

V

L

M

Y

G

A

L

L

R

R

D

E

V

G

L

V

R

R

Y

L

F

V

K

L

E

E

A

E

T

G

L

L

E

E

K

T

I

R

F

F

A

E

E

R

H

H

Q

R

L

H

M

H

S

E

K

A

A

A

T

L

K

A

A

A

A

G

V

I

K

K

A

A

L

M

G

G

L

L

E

R

L

L

F

F

S

G

Q

D

E

D

G

S

A

C

S

K

-

M

D

P

A

V

L

V

T

T

A

C

V

V

R

E

A

I

P

P

A

G

G

G

V

I

D

D

G

G

Q

E

D

S

V

V

V

R

K

D

L

M

L

L

R

L

D

A

K

Q

Y

F

G

G

I

I

M

E

I

I

A

A

G

S

Q

F

A

G

E

P

A

L

K

A

G

G

K

K

I

I

F

W

R

R

I

I

A

G

H

T

M

M

G

G

Y

Y

-

S

I

C

G

R

N

K

F

E

D

N

I

V

V

L

M

F

I

V

I

L

A

A

A

G

L

L

E

E

V

A

V

V

L

L

N

L

E

R

L

H

G

N

Y

A

K

G

A

I

P

E

Y

A

G

G

A

K

G

A

V

L

K

Q

A

A

E

L

E

D

V

V

binding pyridoxal-5'-phosphate: T66 (= T61), S67 (≠ G62), R68 (≠ T63), F93 (= F88), T143 (= T139), D168 (= D165), V170 (≠ I167), Q193 (= Q190), K194 (= K191)

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
33% identity, 92% coverage: 6:357/381 of query aligns to 21:376/377 of 1vjoA

query
sites
1vjoA

L

L

A

G

P

P

G

G

P

P

T

S

P

N

V

A

S

H

P

P

E

S

T

V

L

L

L

Q

A

A

M

M

A

N

T

V

P

S

I

P

I

V

H

G

H

H

R

L

S

D

P

P

Q

A

F

F

A

L

E

A

V

L

V

M

A

D

E

E

C

I

R

Q

V

S

G

L

L

L

K

R

Y

Y

L

V

F

W

Q

Q

T

T

K

E

Q

N

E

P

V

L

L

T

I

I

L

A

A

V

S

S

-

G

T
|
T

G
|
G

T
|
T

G

A

A

A

M

M

E

E

G

A

A

T

I

I

T

A

N

N

T

A

L

V

S

E

P

P

G

G

D

D

T

V

A

V

L

L

V

I

V

G

R

V

G

A

G

G

K

Y

F
|
F

G

G

E

N

R

R

W

L

G

V

E

D

I

M

C

A

A

G

A

R

Y

Y

G

G

V

A

N

D

F

V

E

R

A

T

I

I

D

S

V

K

E

P

W

W

G

G

R

E

A

V

V

F

Q

S

V

L

A

E

D

E

V

L

A

R

A

T

K

A

L

L

K

E

A

T

N

H

-

R

P

P

A

A

I

I

K

L

A

A

V

L

C

-

I

V

Q

H

A

A

H

-

E

E

T
|
T

S

S

T

T

G

G

V

A

N

R

H

Q

P

P

V

L

K

E

E

G

L

V

A

G

E

E

L

L

T

C

K

R

S

E

L

F

P

-

G

G

T

T

L

L

V

V

D
|
D

A

T

I
x
V

S

T

A

S

L

L

G

G

A

G

F

V

E

P

L

I

P

F

M

L

D

D

A

A

W

W

G

G

I

V

D

D

I

L

M

A

V

Y

A

S

G

C

S

S

Q

Q

K
|
K

A

G

M

L

M

G

L

C

P

S

P

P

G

G

L

A

A

S

F

P

A

F

C

T

L

M

S

S

E

S

K

R

A

A

W

I

E

E

F

-

T

-

K

K

T

L

A

Q

T

R

C

R

N

R

K

T

Y

K

Y

V

F

A

N

N

F

W

S

Y

K

L

E

D

L

M

K

N

N

L

I

L

Q

G

K

K

N

Y

T

W

G

G

A

S

-

E

-

R

-

V

-

Y

-

H

Y

H

T

T

S

A

A

P

V

I

S

N

L

L

V

Y

M

Y

G

A

L

L

R

R

D

E

V

A

L

L

R

R

Y

L

F

I

K

A

E

Q

A

E

T

G

L

L

E

A

K

N

I

C

F

W

A

Q

E

R

H

H

Q

Q

L

K

M

N

S

V

K

E

A

Y

T

L

K

W

A

E

A

R

V

L

K

E

A

D

L

I

G

G

L

L

E

S

L

L

F

H

-

V

S

E

Q

K

E

E

G

Y

A

R

S

L

D

P

A

T

L

L

T

T

A

T

V

V

R

C

A

I

P

P

A

D

G

G

V

V

D

D

G

G

Q

K

D

A

V

V

V

A

K

R

L

R

L

L

R

L

D

N

K

E

Y

H

G

N

I

I

M

E

I

V

A

G

G

G

Q

L

A

G

E

E

A

L

K

A

G

G

K

K

I

V

F

W

R

R

I

V

A

G

H

L

M

M

G

G

Y

F

I

N

G

S

N

R

F

K

D

E

I

S

V

V

-

D

M

Q

I

L

I

I

A

P

A

A

L

L

E

E

V

Q

V

V

L

L

binding pyridoxal-5'-phosphate: T77 (= T61), G78 (= G62), T79 (= T63), F104 (= F88), T154 (= T139), D179 (= D165), V181 (≠ I167), K205 (= K191)

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
30% identity, 97% coverage: 6:376/381 of query aligns to 14:406/416 of O32148

query
sites
O32148

L

I

L

M

A

T

P

P

G

G

P

P

T

V

P

E

V

V

S

D

P

P

E

R

T

V

L

L

L

R

A

V

M

M

A

S

T

T

P

P

I

V

H

G

H
x
Q

R

F

S

D

P

P

Q

A

F

F

A

T

E

G

V

I

V

M

A

N

E

E

C

T

R

M

V

E

G

M

L

L

K

R

Y

E

L

L

F

F

Q

Q

T

T

K

K

Q

N

E

R

-

W

V

A

L

Y

I

P

L

I

A

D

S

G

T

T

G

S

T

R

G

A

A

G

M

I

E

E

G

A

A

V

I

L

T

A

N

S

T

V

L

I

S

E

P

P

G

E

D

D

T

D

A

V

L

L

V

I

V

P

R

I

G

Y

G

G

K

R

F

F

G

G

E

Y

R

L

W

L

G

T

E

E

I

I

C

A

A

E

A

R

Y

Y

G

G

V

A

N

N

F

V

E

H

A

M

I

L

D

E

V

C

E

E

W

W

G

G

R

T

A

V

V

F

Q

D

V

P

A

E

D

D

V

I

A

I

A

R

K

E

L

I

K

K

-

K

A

V

N

K

P

P

A

K

I

I

K

-

A

-

V

V

C

A

I

M

Q

V

A

H

H

G

E

E

T

T

S

S

T

T

G

G

V

R

N

I

H

H

P

P

V

L

K

K

E

A

L

I

A

G

E

E

L

A

T

C

K

R

S

T

L

-

P

E

G

D

T

A

L

L

L

F

L

I

V

V

D

D

A

A

I

V

S

A

A

T

L

I

G

G

A

G

F

C

E

E

L

V

P

K

M

V

D

D

A

E

W

W

G

K

I

I

D

D

I

A

M

A

V

I

A

G

G

G

S

T

Q

Q

K
|
K

A

C

M

L

M

S

L

V

P

P

P

S

G

G

L

M

A

A

F

P

A

I

C

T

L

Y

S

N

E

E

K

R

-

V

-

A

-

D

-

V

-

I

-

A

-

R

-

K

-

V

-

E

-

R

-

G

-

I

-

A

-

T

-

Q

-

A

-

D

-

R

-

A

A

A

W

L

E

S

F

G

T

N

K

R

T

P

A

I

T

T

C

S

N

N

K

-

Y

-

Y

Y

F

F

N

D

F

L

S

S

K

Q

-

L

E

E

L

D

K

Y

N

W

I

S

Q

E

K

R

N

R

T

L

G
x
N

A

H

Y

H

T

T

S

E

A

A

V

T

S

T

L

M

V

L

M

Y

G

A

L

L

R

R

D

E

V

G

L

V

R

R

Y

L

F

V

K

L

E

E

A

E

T

G

L

L

E

E

K

T

I

R

F

F

A

E

E

R

H

H

Q

R

L

H

M

H

S

E

K

A

A

A

T

L

K

A

A

A

A

G

V

I

K

K

A

A

L

M

G

G

L

L

E

R

L

L

F

F

S

G

Q

D

E

D

G

S

A

C

S

K

-

M

D

P

A

V

L

V

T

T

A

C

V

V

R

E

A

I

P

P

A

G

G

G

V

I

D

D

G

G

Q

E

D

S

V

V

V

R

K

D

L

M

L

L

R

L

D

A

K

Q

Y

F

G

G

I

I

M

E

I

I

A

A

G

S

Q

F

A

G

E

P

A

L

K

A

G

G

K

K

I

I

F

W

R

R

I

I

A

G

H

T

M

M

G

G

Y

Y

-

S

I

C

G

R

N

K

F

E

D

N

I

V

V

L

M

F

I

V

I

L

A

A

A

G

L

L

E

E

V

A

V

V

L

L

N

L

E

R

L

H

G

N

Y

A

K

G

A

I

P

E

Y

A

G

G

A

K

G

A

V

L

K

Q

A

A

E

L

E

D

V

V

Q37 (≠ H29) mutation to H: 5-fold decrease in transamination activity.
K198 (= K191) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ G236) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

1iugA The crystal structure of aspartate aminotransferase which belongs to subgroup iv from thermus thermophilus (see paper)
34% identity, 93% coverage: 5:360/381 of query aligns to 2:347/348 of 1iugA

query
sites
1iugA

Y

W

L

L

A

T

P

P

G

G

P

P

T

V

P

R

V

L

S

H

P

P

E

K

T

A

L

L

L

E

A

A

M

L

A

A

T

R

P

P

I

Q

I

L

H

H

R

R

S

T

P

E

Q

A

F

A

A

R

E

E

V

V

V

F

A

L

E

K

C

A

R

R

V

G

G

L

L

L

K

R

Y

E

L

A

F

F

Q

R

T

T

K

E

Q

G

E

E

V

V

L

L

I

I

L

L

A

T

S

G

T

S

G

G

T

T

G

L

A

A

M

M

E

E

G

A

A

L

I

V

T

K

N

N

T

L

L

F

S

A

P

P

G

G

D

E

T

R

A

V

L

L

V

V

V

P

R

V

G

Y

G

G

K
|
K

F

F

G

S

E

E

R

R

W

F

G

Y

E

E

I

I

C

A

A

L

A

E

Y

A

G

G

V

L

N

V

F

V

E

E

A

R

I

L

D

D

V

Y

E

P

W

Y

G

G

R

D

A

T

V

P

Q

R

V

P

A

E

D

D

V

V

A

A

A

K

K

E

L

G

K

Y

A

A

N

G

P

-

A

-

I

-

K

-

A

-

V

L

C

L

I

L

Q

V

A

H

H

S

E

E

T

T

S

S

T

T

G

G

V

A

N

L

H

A

P

D

V

L

K

P

E

A

L

L

A

A

E

R

L

A

T

F

K

K

S

E

L

K

-

N

P

P

G

E

T

G

L

L

L

V

L

G

V

A

D

D

A

M

I

V

S

T

A

S

L

L

G

L

A

V

F

G

E

E

L

V

P

A

M

L

D

E

A

A

W

M

G

G

I

V

D

D

I

A

M

A

V

A

A

S

G

G

S

S

Q

Q

K
|
K

A

G

M

L

M

M

L

C

P

P

G

G

L

L

A

G

F

F

A

V

C

A

L

L

S

S

E

P

K

R

A

A

W

L

E

E

F

R

T

L

K

K

T

P

A

-

T

-

C

-

N

R

K

G

Y

Y

F

L

N

D

F

L

S

A

K

R

E

E

L

L

K

K

N

A

I

Q

Q

K

K

E

N

G

T

E

G

S

A

A

Y

W

T

T

S

P

A

A

V

I

S

N

L

L

V

V

M

L

G

A

L

V

R

A

D

A

V

V

L

L

R

-

Y

-

F

-

K

-

E

E

A

E

T

V

L

L

E

P

K

R

I

L

F

-

A

-

E

E

H

E

Q

H

L

L

M

A

S

L

K

K

A

A

T

W

K

Q

A

N

A

A

V

L

K

-

A

-

L

-

G

-

L

L

E

Y

L

G

F

V

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binding phosphate ion: K84 (= K87), K184 (= K191)

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
31% identity, 88% coverage: 6:339/381 of query aligns to 20:360/385 of 5hhyA

query
sites
5hhyA

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binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ T61), G77 (= G62), H78 (≠ T63), W103 (≠ F88), S153 (≠ T139), D178 (= D165), V180 (≠ I167), Q203 (= Q190), K204 (= K191), Y255 (≠ G236), T258 (= T239)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
31% identity, 88% coverage: 6:339/381 of query aligns to 25:365/392 of P21549

query
sites
P21549

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R36 (≠ E17) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ M22) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (≠ H28) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G62) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F88) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ W92) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ K130) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ V132) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ A136) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T139) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G142) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ V147) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ A151) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ T154) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D165) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (≠ A169) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (= I184) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (≠ A187) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K191) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ A200) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ K213) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ F224) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (vs. gap) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ F255) to T: in dbSNP:rs140992177
A280 (≠ K256) to V: in dbSNP:rs73106685
V326 (≠ A300) to I: in dbSNP:rs115057148
I340 (≠ R314) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
31% identity, 88% coverage: 6:339/381 of query aligns to 20:360/384 of 6rv0A

query
sites
6rv0A

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P

G

G

L

T

A

S

F

L

A

I

C

S

L

F

S

S

E

D

K

K

A

A

W

K

E

K

-

K

-

M

F

Y

T

S

K

R

T

K

A

T

T

K

C

P

N

F

K

S

Y

F

Y

Y

F

L

N

D

F

I

S

-

K

K

E

W

L

L

K

A

N

N

I

F

-

W

-

G

-

C

-

D

Q

Q

K

P

N

R

T

M

G

Y

A

H

Y

H

T

T

S

I

A

P

V

V

S

I

L

S

V

L

M

Y

G

S

L

L

R

R

D

E

V

S

L

L

R

A

Y

L

F

I

K

A

E

E

A

Q

T

G

L

L

E

E

K

N

I

S

F

W

A

R

E

Q

H

H

Q

R

L

E

M

A

S

A

K

A

A

Y

T

L

K

H

A

G

A

R

V

L

K

Q

A

A

L

L

G

G

L

L

E

Q

L

L

F

F

S

V

Q

K

E

D

G

P

A

A

-

L

-

R

S

L

D

P

A

T

L

V

T

T

A

T

V

V

R

A

A

V

P

P

A

A

G

G

V

Y

D

D

G

W

Q

R

D

D

V

I

V

V

K

S

L

Y

L

V

R

I

D

D

K

H

Y

F

G

D

I

I

M

E

I

I

A

M

G

G

Q

L

A

G

E

P

A

S

K

T

G

G

K

K

I

V

F

L

R

R

I

I

A

G

H

L

M

L

G

G

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (≠ T61), G77 (= G62), H78 (≠ T63), W103 (≠ F88), D178 (= D165), V180 (≠ I167), A181 (≠ S168), K204 (= K191)

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
31% identity, 88% coverage: 6:339/381 of query aligns to 22:362/387 of 1j04A

query
sites
1j04A

L

L

A

G

P
|
P

G

G

P

P

T

S

P

N

V

L

S

P

P

P

E

R

T

I

L

M

L

A

A

A

M

G

A

G

T

L

P

Q

I

M

I

I

H

G

H

S

R

M

S

S

P

K

Q

D

F

M

A

Y

E

Q

V

I

V

M

A

D

E

E

C

I

R

K

V

E

G

G

L

I

K

Q

Y

Y

L

V

F

F

Q

Q

T

T

K

R

Q

N

E

P

V

L

-

T

L

L

I

V

L

I

A

S

S

G

T

S

G

G

T

H

G

C

A

A

M

L

E

E

G

A

A

A

I

L

T

V

N

N

T

V

L

L

S

E

P

P

G

G

D

D

T

S

A

F

L

L

V

V

V

G

R

A

G

N

G

G

K

I

F

W

G

G

E

Q

R

R

W

A

G

V

E

D

I

I

C

G

A

E

A

R

Y

I

G

G

V

A

N

R

F

V

E

H

A

P

I

M

D

T

V

K

E

D

W

P

G

G

R

G

A

H

V

Y

Q

T

V

L

A

Q

D

E

V

V

A

E

K

G

L

L

K

A

A

Q

N

H

P

K

A

P

I

V

K

L

A

L

V

F

C

L

I

T

Q

H

A

G

H

-

E

E

T

S

S

S

T

T

G

G

V

V

N

L

H

Q

P

P

V

L

K

D

E

G

L

F

A

R

E

E

L

L

T

C

K

H

S

R

L

Y

P

-

G

K

T

C

L

L

V

V

D

D

A

S

I

V

S

A

A

S

L

L

G

G

A

G

F

T

E

P

L

L

P

Y

M

M

D

D

A

R

W

Q

G

G

I

I

D

D

I

I

M

L

V

Y

A

S

G

G

S

S

Q

Q

K

K

A

A

M

L

M

N

L

A

P

P

G

G

L

T

A

S

F

L

A

I

C

S

L

F

S

S

E

D

K

K

A

A

W

K

E

K

-

K

-

M

F

Y

T

S

K

R

T

K

A

T

T

K

C

P

N

F

K

S

Y

F

Y

Y

F

L

N

D

F

I

S

-

K

K

E

W

L

L

K

A

N

N

I

F

-

W

-

G

-

C

-

D

Q

Q

K

P

N

R

T

M

G

Y

A

H

Y

H

T

T

S

I

A

P

V

V

S

I

L

S

V

L

M

Y

G

S

L

L

R

R

D

E

V

S

L

L

R

A

Y

L

F

I

K

A

E

E

A

Q

T

G

L

L

E

E

K

N

I

S

F

W

A

R

E

Q

H

H

Q

R

L

E

M

A

S

A

K

A

A

Y

T

L

K

H

A

G

A

R

V

L

K

Q

A

A

L

L

G

G

L

L

E

Q

L

L

F

F

S

V

Q

K

E

D

G

P

A

A

-

L

-

R

S

L

D

P

A

T

L

V

T

T

A

T

V

V

R

A

A

V

P

P

A

A

G

G

V

Y

D

D

G

W

Q

R

D

D

V

I

V

V

K

S

L

Y

L

V

R

I

D

D

K

H

Y

F

G

D

I

I

M

E

I

I

A

M

G

G

Q

L

A

G

E

P

A

S

K

T

G

G

K

K

I

V

F

L

R
|
R

I

I

A

G

H

L

M

L

G

G

binding (aminooxy)acetic acid: P25 (= P9), R357 (= R334)

3zrqA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
32% identity, 98% coverage: 6:378/381 of query aligns to 4:380/382 of 3zrqA

query
sites
3zrqA

L

L

A

H

P

V

G

G

P

P

T

T

P

T

V

I

S

K

P

E

E

D

T

V

L

L

L

V

A

A

M

G

A

L

T

E

P

N

I

N

I

V

H

G

H

F

R

T

S

S

P

K

Q

E

F

F

A

V

E

E

V

A

V

L

A

A

E

Y

C

S

R

L

V

K

G

G

L

L

K

R

Y

Y

L

V

F

M

Q

G

T

A

K

S

Q

K

-

N

-

Y

E

Q

V

P

L

L

I

I

L

I

A

P

S

G

T

G

G
|
G

T
|
T

G

S

A

A

M

M

E

E

G

-

A

S

I

V

T

T

N

S

T

L

L

L

S

K

P

P

G

N

D

D

T

K

A

I

L

L

V

V

R

S

G

N

G

G

K

V

F
|
F

G

G

E

D

R

R

W

W

G

E

E

Q

I

I

C

F

A

K

A

R

Y

Y

G

P

V

V

N

N

F

V

E

K

A

V

I

L

D

R

V

P

E

S

W

P

G

G

R

D

A

Y

V

V

Q

K

V

P

A

G

D

E

V

V

A

E

K

E

L

V

K

R

A

K

N

S

P

-

A

E

I

Y

K

K

A

L

V

V

C

A

I

L

Q

T

A

H

H

V

E

E

T
|
T

S

S

T

T

G

G

V

V

N

R

H

E

P

P

V

V

K

K

E

D

L

V

A

I

E

N

L

K

T

I

K

R

S

K

L

Y

P

V

G

-

T

E

L

L

L

I

L

V

V

V

D
|
D

A

G

I
x
V

S

S

A

S

L

V

G

G

A

A

F

E

E

E

L

V

P

K

M

A

D

E

A

E

W

W

G

N

I

V

D

D

I

V

M

Y

V

L

A

T

G

A

S

S

Q

Q

K
|
K

A

A

M

L

M

G

L

S

P

A

G

G

L

L

A

G

F

L

A

L

C

L

L

L

S

S

E

P

K

K

A

A

W

L

E

S

F

I

T

L

K

D

T

S

A

Q

T

N

C

S

N

I

K

A

Y

G

Y

Y

F

Y

-

L

-

D

-

L

-

R

N

N

F

W

S

L

K

P

E

V

L

M

K

R

N

G

I

A

Q

E

K

E

N

G

T

K

G

A

A

A

Y
|
Y

-

F

-

A

T
|
T

S

P

A

P

V

V

S

H

L

V

V

I

M

L

G

Q

L

L

R

A

D

E

V

A

L

F

R

R

Y

L

F

I

K

E

E

K

A

E

T

G

L

I

E

E

K

N

I

R

F

I

A

K

E

R

H

H

Q

T

L

M

M

V

S

A

K

S

A

A

T

I

K

R

A

A

A

G

V

L

K

E

A

A

L

L

G

G

L

L

E

E

L

I

F

V

S

A

Q

R

-

R

-

P

E

E

G

S

A

Y

S

S

D

N

A

T

L

V

T

T

A

G

V

V

R

I

A

L

P

K

A

V

G

A

V

-

D

D

G

P

Q

Q

D

K

V

V

V

L

K

A

L

G

L

T

R

V

D

N

K

E

Y

-

G

G

I

V

M

E

I

F

A

A

G

P

G

G

Q

V

A

H

E

P

A

A

K

-

G

F

K

K

I

Y

F

F

R

R

I

I

A

G

H

H

M

M

G

G

Y

W

I

V

G

T

N

P

F

N

D

D

I

A

V

I

M

I

I

A

I

I

A

S

A

V

L

I

E

E

V

R

V

T

L

L

N

R

E

K

L

L

G

G

Y

E

K

P

A

I

P

R

Y

F

G

G

A

E

G

G

V

V

K

K

A

A

A

V

E

E

V

V

L

L

F

F

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G62 (= G62), T63 (= T63), F87 (= F88), T137 (= T139), D162 (= D165), V164 (≠ I167), K188 (= K191), Y239 (= Y238), T242 (= T239)

3zrrA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
32% identity, 98% coverage: 6:378/381 of query aligns to 1:377/379 of 3zrrA

query
sites
3zrrA

L

L

A

H

P
x
V

G

G

P

P

T

T

P

T

V

I

S

K

P

E

E

D

T

V

L

L

L

V

A

A

M

G

A

L

T

E

P

N

I

N

I

V

H

G

H
x
F

R

T

S

S

P

K

Q

E

F

F

A

V

E

E

V

A

V

L

A

A

E

Y

C

S

R

L

V

K

G

G

L

L

K

R

Y

Y

L

V

F

M

Q

G

T

A

K

S

Q

K

-

N

-

Y

E

Q

V

P

L

L

I

I

L

I

A

P

S

G

T
x
G

G
|
G

T
|
T

G

S

A

A

M

M

E

E

G

-

A

S

I

V

T

T

N

S

T

L

L

L

S

K

P

P

G

N

D

D

T

K

A

I

L

L

V

V

R

S

G

N

G

G

K

V

F
|
F

G

G

E

D

R

R

W

W

G

E

E

Q

I

I

C

F

A

K

A

R

Y

Y

G

P

V

V

N

N

F

V

E

K

A

V

I

L

D

R

V

P

E

S

W

P

G

G

R

D

A

Y

V

V

Q

K

V

P

A

G

D

E

V

V

A

E

K

E

L

V

K

R

A

K

N

S

P

-

A

E

I

Y

K

K

A

L

V

V

C

A

I

L

Q

T

A

H

H

V

E

E

T
|
T

S

S

T

T

G

G

V

V

N

R

H

E

P

P

V

V

K

K

E

D

L

V

A

I

E

N

L

K

T

I

K

R

S

K

L

Y

P

V

G

-

T

E

L

L

L

I

L

V

V

V

D
|
D

A

G

I
x
V

S

S

A

S

L

V

G

G

A

A

F

E

E

E

L

V

P

K

M

A

D

E

A

E

W

W

G

N

I

V

D

D

I

V

M

Y

V

L

A

T

G

A

S

S

Q

Q

K

K

A

A

M

L

M

G

L

S

P

A

G

G

L

L

A

G

F

L

A

L

C

L

L

L

S

S

E

P

K

K

A

A

W

L

E

S

F

I

T

L

K

D

T

S

A

Q

T

N

C

S

N

I

K

A

Y

G

Y

Y

F

Y

-

L

-

D

-

L

-

R

N

N

F

W

S

L

K

P

E

V

L

M

K

R

N

G

I

A

Q

E

K

E

N

G

T

K

G

A

A

A

Y
|
Y

-

F

-

A

T
|
T

S

P

A

P

V

V

S

H

L

V

V

I

M

L

G

Q

L

L

R

A

D

E

V

A

L

F

R

R

Y

L

F

I

K

E

E

K

A

E

T

G

L

I

E

E

K

N

I

R

F

I

A

K

E

R

H

H

Q

T

L

M

M

V

S

A

K

S

A

A

T

I

K

R

A

A

A

G

V

L

K

E

A

A

L

L

G

G

L

L

E

E

L

I

F

V

S

A

Q

R

-

R

-

P

E

E

G

S

A

Y

S

S

D

N

A

T

L

V

T

T

A

G

V

V

R

I

A

L

P

K

A

V

G

A

V

-

D

D

G

P

Q

Q

D

K

V

V

V

L

K

A

L

G

L

T

R

V

D

N

K

E

Y

-

G

G

I

V

M

E

I

F

A

A

G

P

G

G

Q

V

A

H

E

P

A

A

K

-

G

F

K

K

I

Y

F

F

R
|
R

I

I

A

G

H

H

M

M

G

G

Y

W

I

V

G

T

N

P

F

N

D

D

I

A

V

I

M

I

I

A

I

I

A

S

A

V

L

I

E

E

V

R

V

T

L

L

N

R

E

K

L

L

G

G

Y

E

K

P

A

I

P

R

Y

F

G

G

A

E

G

G

V

V

K

K

A

A

A

V

E

E

V

V

L

L

F

F

binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: V4 (≠ P9), F24 (≠ H29), G58 (≠ T61), G59 (= G62), T60 (= T63), F84 (= F88), T134 (= T139), D159 (= D165), V161 (≠ I167), Y236 (= Y238), T239 (= T239), R333 (= R334)

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
32% identity, 88% coverage: 6:339/381 of query aligns to 22:360/385 of 1h0cA

query
sites
1h0cA

L

L

A

G

P
|
P

G
|
G

P

P

T

S

P

N

V

L

S

P

P

P

E

R

T

I

L

M

L

A

A

A

M

G

A

G

T

L

P

Q

I

M

I

I

H

G

H

S

R

M

S

S

P

K

Q

D

F

M

A

Y

E

Q

V

I

V

M

A

D

E

E

C

I

R

K

V

E

G

G

L

I

K

Q

Y

Y

L

V

F

F

Q

Q

T

T

K

R

Q

N

E

P

V

L

-

T

L

L

I

V

L

I

A

S

S

G

T
x
S

G
|
G

T
x
H

G

C

A

A

M

L

E

E

G

A

A

A

I

L

T

V

N

N

T

V

L

L

S

E

P

P

G

G

D

D

T

S

A

F

L

L

V

V

V

G

R

A

G

N

G

G

K

I

F
x
W

G

G

E

Q

R

R

W

A

G

V

E

D

I

I

C

G

A

E

A

R

Y

I

G

G

V

V

N

H

F

P

E

M

A

T

I

K

D

D

V

P

E

-

W

-

G

G

R

G

A

H

V

Y

Q

T

V

L

A

Q

D

E

V

V

A

E

K

G

L

L

K

A

A

Q

N

H

P

K

A

P

I

V

K

L

A

L

V

F

C

L

I

T

Q

H

A

G

H

-

E

E

T
x
S

S

S

T

T

G

G

V

V

N

L

H

Q

P

P

V

L

K

D

E

G

L

F

A

G

E

E

L

L

T

C

K

H

S

R

L

Y

P

-

G

K

T

C

L

L

V

V

D
|
D

A

S

I
x
V

S

A

A

S

L

L

G

G

A

G

F

T

E

P

L

L

P

Y

M

M

D

D

A

R

W

Q

G

G

I

I

D

D

I

I

M

L

V

Y

A

S

G

G

S

S

Q

Q

K
|
K

A

A

M

L

M

N

L

A

P

P

G

G

L

T

A

S

F

L

A

I

C

S

L

F

S

S

E

D

K

K

A

A

W

K

E

K

-

K

-

M

F

Y

T

S

K

R

T

K

A

T

T

K

C

P

N

F

K

S

Y

F

Y

Y

F

L

N

D

F

I

S

-

K

K

E

W

L

L

K

A

N

N

I

F

-

W

-

G

-

C

-

D

Q

Q

K

P

N

R

T

M

G

Y

A

H

Y

H

T

T

S

I

A

P

V

V

S

I

L

S

V

L

M

Y

G

S

L

L

R

R

D

E

V

S

L

L

R

A

Y

L

F

I

K

A

E

E

A

Q

T

G

L

L

E

E

K

N

I

S

F

W

A

R

E

Q

H

H

Q

R

L

E

M

A

S

A

K

A

A

Y

T

L

K

H

A

G

A

R

V

L

K

Q

A

A

L

L

G

G

L

L

E

Q

L

L

F

F

S

V

Q

K

E

D

G

P

A

A

-

L

-

R

S

L

D

P

A

T

L

V

T

T

A

T

V

V

R

A

A

V

P

P

A

A

G

G

V

Y

D

D

G

W

Q

R

D

D

V

I

V

V

K

S

L

Y

L

V

R

I

D

D

K

H

Y

F

G

D

I

I

M

E

I

I

A

M

G

G

Q
x
L

A

G

E

P

A

S

K

T

G

G

K

K

I

V

F

L

R
|
R

I

I

A

G

H

L

M

L

G

G

binding (aminooxy)acetic acid: P25 (= P9), G26 (= G10), L346 (≠ Q325), R355 (= R334)
binding pyridoxal-5'-phosphate: S78 (≠ T61), G79 (= G62), H80 (≠ T63), W105 (≠ F88), S153 (≠ T139), D178 (= D165), V180 (≠ I167), K204 (= K191)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

3zrpA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
31% identity, 97% coverage: 10:378/381 of query aligns to 4:376/377 of 3zrpA

query
sites
3zrpA

G

G

P

P

T

T

P

T

V

I

S

K

P

E

E

D

T

V

L

L

L

V

A

A

M

G

A

L

T

E

P

N

I

N

I

V

H

G

H

F

R

T

S

S

P

K

Q

E

F

F

A

V

E

E

V

A

V

L

A

A

E

Y

C

S

R

L

V

K

G

G

L

L

K

R

Y

Y

L

V

F

M

Q

G

T

A

K

S

Q

K

-

N

-

Y

E

Q

V

P

L

L

I

I

L

I

A

P

S

G

T
x
G

G
|
G

T
|
T

G

S

A

A

M

M

E

E

G

-

A

S

I

V

T

T

N

S

T

L

L

L

S

K

P

P

G

N

D

D

T

K

A

I

L

L

V

V

R

S

G

N

G

G

K

V

F
|
F

G

G

E

D

R

R

W

W

G

E

E

Q

I

I

C

F

A

K

A

R

Y

Y

G

P

V

V

N

N

F

V

E

K

A

V

I

L

D

R

V

P

E

S

W

P

G

G

R

D

A

Y

V

V

Q

K

V

P

A

G

D

E

V

V

A

E

K

E

L

V

K

R

A

K

N

S

P

-

A

E

I

Y

K

K

A

L

V

V

C

A

I

L

Q

T

A

H

H

V

E

E

T
|
T

S

S

T

T

G

G

V

V

N

R

H

E

P

P

V

V

K

K

E

D

L

V

A

I

E

N

L

K

T

I

K

R

S

K

L

Y

P

V

G

-

T

E

L

L

L

I

L

V

V

V

D
|
D

A

G

I
x
V

S

S

A

S

L

V

G

G

A

A

F

E

E

E

L

V

P

K

M

A

D

E

A

E

W

W

G

N

I

V

D

D

I

V

M

Y

V

L

A

T

G

A

S

S

Q

Q

K
|
K

A

A

M

L

M

G

L

S

P

A

G

G

L

L

A

G

F

L

A

L

C

L

L

L

S

S

E

P

K

K

A

A

W

L

E

S

F

I

T

L

K

D

T

S

A

Q

T

N

C

S

N

I

K

A

Y

G

Y

Y

F

Y

-

L

-

D

-

L

-

R

N

N

F

W

S

L

K

P

E

V

L

M

K

R

N

G

I

A

Q

E

K

E

N

G

T

K

G

A

A

A

Y
|
Y

-

F

-

A

T
|
T

S

P

A

P

V

V

S

H

L

V

V

I

M

L

G

Q

L

L

R

A

D

E

V

A

L

F

R

R

Y

L

F

I

K

E

E

K

A

E

T

G

L

I

E

E

K

N

I

R

F

I

A

K

E

R

H

H

Q

T

L

M

M

V

S

A

K

S

A

A

T

I

K

R

A

A

A

G

V

L

K

E

A

A

L

L

G

G

L

L

E

E

L

I

F

V

S

A

Q

R

-

R

-

P

E

E

G

S

A

Y

S

S

D

N

A

T

L

V

T

T

A

G

V

V

R

I

A

L

P

K

A

V

G

A

V

-

D

D

G

P

Q

Q

D

K

V

V

V

L

K

A

L

G

L

T

R

V

D

N

K

E

Y

-

G

G

I

V

M

E

I

F

A

A

G

P

G

G

Q

V

A

H

E

P

A

A

K

-

G

F

K

K

I

Y

F

F

R

R

I

I

A

G

H

H

M

M

G

G

Y

W

I

V

G

T

N

P

F

N

D

D

I

A

V

I

M

I

I

A

I

I

A

S

A

V

L

I

E

E

V

R

V

T

L

L

N

R

E

K

L

L

G

G

Y

E

K

P

A

I

P

R

Y

F

G

G

A

E

G

G

V

V

K

K

A

A

A

V

E

E

V

V

L

L

F

F

binding pyridoxal-5'-phosphate: G57 (≠ T61), G58 (= G62), T59 (= T63), F83 (= F88), T133 (= T139), D158 (= D165), V160 (≠ I167), K184 (= K191), Y235 (= Y238), T238 (= T239)

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
29% identity, 88% coverage: 6:340/381 of query aligns to 25:364/388 of 3kgwB

query
sites
3kgwB

L

L

A

G

P

P

G

G

P

P

T

S

P

N

V

L

S

A

P

P

E

R

T

V

L

L

L

A

A

A

M

G

A

S

T

L

P

R

I

M

I

I

H

G

H

H

R

M

S

Q

P

K

Q

E

F

M

A

L

E

Q

V

I

V

M

A

E

E

E

C

I

R

K

V

Q

G

G

L

I

K

Q

Y

Y

L

V

F

F

Q

Q

T

T

K

R

Q

N

E

P

V

L

-

T

L

L

I

V

L

V

A

S

S

G

T
x
S

G
|
G

T
x
H

G

C

A

A

M

M

E

E

G

T

A

A

I

L

T

F

N

N

T

L

L

L

S

E

P

P

G

G

D

D

T

S

A

F

L

L

V

T

V

G

R

T

G

N

G

G

K

I

F
x
W

G

G

E

M

R

R

W

A

G

A

E

E

I

I

C

A

A

D

A

R

Y

I

G

G

V

A

N

R

F

V

E

H

A

Q

I

M

D

I

V

K

E

K

W

P

G

G

R

E

A

H

V

Y

Q

T

V

L

A

Q

D

E

V

V

A

E

K

G

L

L

K

A

A

Q

N

H

P

K

A

P

I

V

K

L

A

L

V

F

C

L

I

V

Q

H

A

G

H

-

E

E

T
x
S

S

S

T

T

G

G

V

V

N

V

H

Q

P

P

V

L

K

D

E

G

L

F

A

G

E

E

L

L

T

C

K

H

S

R

L

Y

P

-

G

Q

T

C

L

L

V

V

D
|
D

A

S

I
x
V

S

A

A

S

L

L

G

G

A

G

F

V

E

P

L

I

P

Y

M

M

D

D

A

Q

W

Q

G

G

I

I

D

D

I

I

M

M

V

Y

A

S

G

S

S

S

Q

Q

K
|
K

A

V

M

L

M

N

L

A

P

P

G

G

L

I

A

S

F

L

A

I

C

S

L

F

S

N

E

D

K

K

A

A

W

K

E

Y

-

K

-

V

F

Y

T

S

K

R

T

K

A

T

T

K

C

P

N

V

K

S

Y

F

Y

Y

F

T

N

D

F

I

S

T

-

Y

-

L

K

A

E

K

L

L

K

W

N

G

I

C

Q

E

K

T

N

R

T

V

G

I

A

H

Y

H

T

T

S

T

A

P

V

V

S

T

L

S

V

L

M

Y

G

C

L

L

R

R

D

E

V

S

L

L

R

A

Y

L

F

I

K

A

E

E

A

Q

T

G

L

L

E

E

K

N

I

C

F

W

A

R

E

R

H

H

Q

R

L

E

M

A

S

T

K

A

A

H

T

L

K

H

A

K

A

H

V

L

K

Q

A

E

L

M

G

G

L

L

E

K

L

F

F

F

S

V

Q

K

-

D

-

P

E

E

G

I

A

R

S

L

D

P

A

T

L

I

T

T

A

T

V

V

R

T

A

V

P

P

A

A

G

G

V

Y

D

N

G

W

Q

R

D

D

V

I

V

V

K

S

L

Y

L

V

R

L

D

D

K

H

Y

F

G

S

I

I

M

E

I

I

A

S

G

G

Q

L

A

G

E

P

A

T

K

E

G

E

K

R

I

V

F

L

R

R

I

I

A

G

H

L

M

L

G

G

Y

Y

binding pyridoxal-5'-phosphate: S81 (≠ T61), G82 (= G62), H83 (≠ T63), W108 (≠ F88), S158 (≠ T139), D183 (= D165), V185 (≠ I167), K209 (= K191)

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
29% identity, 88% coverage: 6:340/381 of query aligns to 21:359/383 of 3kgxA

query
sites
3kgxA

L

L

A

G

P

P

G

G

P

P

T

S

P

N

V

L

S

A

P

P

E

R

T

V

L

L

L

A

A

A

M

G

A

S

T

L

P

R

I

M

I

I

H

G

H

H

R

M

S

Q

P

K

Q

E

F

M

A

L

E

Q

V

I

V

M

A

E

E

E

C

I

R

K

V

Q

G

G

L

I

K

Q

Y

Y

L

V

F

F

Q

Q

T

T

K

R

Q

N

E

P

V

L

-

T

L

L

I

V

L

V

A

S

S

G

T

S

G

G

T

H

G

C

A

A

M

M

E

E

G

T

A

A

I

L

T

F

N

N

T

L

L

L

S

E

P

P

G

G

D

D

T

S

A

F

L

L

V

T

V

G

R

T

G

N

G

G

K

I

F

W

G

G

E

M

R

R

W

A

G

A

E

E

I

I

C

A

A

D

A

R

Y

I

G

G

V

A

N

R

F

V

E

H

A

Q

I

M

D

I

V

K

E

K

W

P

G

G

R

E

A

H

V

Y

Q

T

V

L

A

Q

D

E

V

V

A

E

K

G

L

L

K

A

A

Q

N

H

P

K

A

P

I

V

K

L

A

L

V

F

C

L

I

V

Q

H

A

G

H

-

E

E

T

S

S

S

T

T

G

G

V

V

N

V

H

Q

P

P

V

L

K

D

E

G

L

F

A

G

E

E

L

L

T

C

K

H

S

R

L

Y

P

-

G

Q

T

C

L

L

V

V

D

D

A

S

I

V

S

A

A

S

L

L

G

G

A

G

F

V

E

P

L

I

P

Y

M

M

D

D

A

Q

W

Q

G

G

I

I

D

D

I

I

M

M

V

Y

A

S

G

S

S

S

Q

Q

K

K

A

V

M

L

M

N

L

A

P

P

G

G

L

I

A

S

F

L

A

I

C

S

L

F

S

N

E

D

K

K

A

A

W

-

E

-

F

K

T

Y

K

K

T

V

A

Y

T

S

C

R

N

K

K

T

Y

K

Y

P

F

V

N

S

F

F

S

Y

K

T

E

D

L

I

K

T

N

Y

I

L

Q

A

K

K

N

L

T

W

G

G

A

C

-

E

-

G

-

V

-

I

-

H

Y

H

T

T

S

T

A

P

V

V

S

T

L

S

V

L

M

Y

G

C

L

L

R

R

D

E

V

S

L

L

R

A

Y

L

F

I

K

A

E

E

A

Q

T

G

L

L

E

E

K

N

I

C

F

W

A

R

E

R

H

H

Q

R

L

E

M

A

S

T

K

A

A
x
H

T

L

K

H

A

K

A
x
H

V

L

K

Q

A

E

L

M

G

G

L

L

E

K

L

F

F

F

S

V

Q

K

-

D

-

P

E

E

G

I

A

R

S

L

D

P

A

T

L

I

T

T

A

T

V

V

R

T

A

V

P

P

A

A

G

G

V

Y

D

N

G

W

Q

R

D

D

V

I

V

V

K

S

L

Y

L

V

R

L

D

D

K

H

Y

F

G

S

I

I

M

E

I

I

A

S

G

G

Q

L

A

G

E

P

A

T

K

E

G

E

K

R

I

V

F

L

R

R

I

I

A

G

H

L

M

L

G

G

Y

Y

binding magnesium ion: H290 (≠ A273), H294 (≠ A277)

Sites not aligning to the query:

binding magnesium ion: 367

Q988B8 Pyridoxamine--pyruvate transaminase; Pyridoxamine-pyruvate aminotransferase; EC 2.6.1.30 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
30% identity, 97% coverage: 7:377/381 of query aligns to 14:389/393 of Q988B8

query
sites
Q988B8

L

L

A

T

P

A

G

G

P

P

T

V

P

N

V

A

S

Y

P

P

E

E

T

V

L

L

L

R

A

G

M

L

A

G

T

R

P

T

I

V

I

L

H

Y

H

D

R

Y

S

D

P

P

Q

A

F

F

A

Q

E

L

V

L

V

Y

A

E

E

K

C

V

R

V

V

D

G

K

L

A

K

Q

Y

K

L

A

F

M

Q

R

T

L

K

S

Q

N

E

K

V

P

L

V

I

I

L

L

A

H

S

G

T
x
E

G

P

T

V

G

L

A

G

M

L

E

E

G

A

A

A

I

A

T

A

N

S

T

L

L

I

S

S

P

P

G

D

D

D

T

V

A

V

L

L

V

N

V

L

R

A

G

S

G

G

K

V

F
x
Y

G

G

E

K

R

G

W

F

G

G

E

Y

I

W

C

A

A

K

A

R

Y

Y

G

S

V

P

N

H

F

L

E

L

A

E

I

I

D

E

V

V

E

P

W

Y

G

N

R

E

A

A

V

I

Q

D

V

P

A

Q

D

A

V

V

A

A

A

D

K

M

L

L

K

K

A

A

N

H

P

P

A

E

I

I

K

T

A

V

V

V

C

S

I

V

Q

C

A

H

H

H

E

D

T
|
T

S

P

T

S

G

G

V

T

N

I

H

N

P

P

V

I

K

D

E

A

L

I

A

G

E

A

L

L

T

V

K

-

S

S

L

A

P

H

G

G

T

A

L

Y

L

L

L

I

V

V

D

D

A

A

I

V

S

S

A

S

L

F

G

G

A

G

F

M

E

K

L

T

P

H

M

P

D

E

A

D

W

C

G

K

I

A

D

D

I

I

M

Y

V

V

A

T

G

G

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P

Q

N

K
|
K

A
x
C

M

L

M

G

L

A

P

P

G

G

L

L

A

T

F

M

A

M

C

G

L

V

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S

E

E

K

R

A

A

W

W

E

A

F

K

T

M

K

K

T

A

A

N

T

P

C

L

N

A

K

P

Y

R

Y

A

F

S

N

M

F

L

S

S

-

I

K

V

E

D

L

W

K

E

N

N

I

A

-

W

-

S

Q

R

K

D

N

K

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P

G

F

A

P

Y

F

T

T

S

P

A

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V

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S

L

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M

N

G

G

L

L

R

D

D

V

V

A

L

L

R

D

Y

L

F

Y

K

L

E

N

A

E

T

G

L

P

E

E

K

A

I

V

F

W

A

A

E

R

H

H

Q

A

L

L

M

T

S

A

K

K

A

A

T

M

K

R

A

A

A

G

V

V

K

T

A

A

L

M

G

G

L

L

E

S

L

V

F

W

-

A

S

S

Q

D

E

S

G

I

A

A

S

S

D

P

A

T

L

T

T

T

A

A

V

V

R

R

A

T

P

P

A

D

G

G

V

V

D

D

G

E

Q

K

D

A

V

L

V

R

K

Q

L

A

R

R

D

A

K

R

Y

Y

G

G

I

V

M

V

I

F

A

S

G

S

G

G

Q
x
R

A

G

E

E

A

T

K

L

G

G

K

K

I

L

F

T

R
|
R

I

I

A

G

H

H

M

M

G

G

Y

P

I

T

G

A

N

Q

F

-

D

-

I

P

V

I

M

Y

I

A

I

I

A

A

L

L

E

T

V

A

V

L

-

G

-

A

L

M

N

N

E

A

L

A

G

G

Y

R

K

K

A

L

P

A

Y

I

G

G

A

K

G

G

V

I

K

E

A

A

E

L

E

A

V

V

L

I

E68 (≠ T61) binding pyridoxal 5'-phosphate; mutation E->A,G: Low but detectable pyridoxamine--pyruvate transaminase activity.
Y95 (≠ F88) binding pyridoxal 5'-phosphate
T146 (= T139) binding pyridoxal 5'-phosphate
K197 (= K191) modified: N6-(pyridoxal phosphate)lysine; mutation to L: Loss of function.
C198 (≠ A192) mutation to A: No effect on enzyme activity.
R336 (≠ Q325) mutation to A: Strongly decreased affinity for pyruvate.
R345 (= R334) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2z9xA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxyl-l-alanine (see paper)
30% identity, 97% coverage: 7:377/381 of query aligns to 13:388/392 of 2z9xA

query
sites
2z9xA

L

L

A

T

P

A

G

G

P

P

T

V

P

N

V

A

S

Y

P

P

E

E

T

V

L

L

L

R

A

G

M

L

A

G

T

R

P

T

I

V

I

L

H

Y

H

D

R

Y

S

D

P

P

Q

A

F

F

A

Q

E

L

V

L

V

Y

A

E

E

K

C

V

R

V

V

D

G

K

L

A

K

Q

Y

K

L

A

F

M

Q

R

T

L

K

S

Q

N

E

K

V

P

L

V

I

I

L

L

A

H

S

G

T
x
E

G

P

T

V

G

L

A

G

M

L

E

E

G

A

A

A

I

A

T

A

N

S

T

L

L

I

S

S

P

P

G

D

D

D

T

V

A

V

L

L

V

N

V

L

R

A

G

S

G

G

K

V

F
x
Y

G

G

E

K

R

G

W

F

G

G

E

Y

I

W

C

A

A

K

A

R

Y

Y

G

S

V

P

N

H

F

L

E

L

A

E

I

I

D

E

V

V

E

P

W

Y

G

N

R

E

A

A

V

I

Q

D

V

P

A

Q

D

A

V

V

A

A

A

D

K

M

L

L

K

K

A

A

N

H

P

P

A

E

I

I

K

T

A

V

V

V

C

S

I

V

Q

C

A

H

H

H

E

D

T
|
T

S

P

T

S

G

G

V

T

N

I

H

N

P

P

V

I

K

D

E

A

L

I

A

G

E

A

L

L

T

V

K

-

S

S

L

A

P

H

G

G

T

A

L

Y

L

L

L

I

V

V

D
|
D

A

A

I
x
V

S

S

A

S

L

F

G

G

A

G

F

M

E

K

L

T

P

H

M

P

D

E

A

D

W

C

G

K

I

A

D

D

I

I

M

Y

V

V

A

T

G

G

S

P

Q

N

K
|
K

A

C

M

L

M

G

L

A

P

P

G

G

L

L

A

T

F

M

A

M

C

G

L

V

S

S

E

E

K

R

A

A

W

W

E

A

F

K

T

M

K

K

T

A

A

N

T

P

C

L

N

A

K

P

Y

R

Y

A

F

S

N

M

F

L

S

S

-

I

K

V

E

D

L

W

K

E

N

N

I

A

-

W

-

S

Q

R

K

D

N

K

T

P

G

F

A

P

Y

F

T

T

S

P

A

S

V

V

S

S

L

E

V

I

M

N

G

G

L

L

R

D

D

V

V

A

L

L

R

D

Y

L

F

Y

K

L

E

N

A

E

T

G

L

P

E

E

K

A

I

V

F

W

A

A

E

R

H

H

Q

A

L

L

M

T

S

A

K

K

A

A

T

M

K

R

A

A

A

G

V

V

K

T

A

A

L

M

G

G

L

L

E

S

L

V

F

W

-

A

S

S

Q

D

E

S

G

I

A

A

S

S

D

P

A

T

L

T

T

T

A

A

V

V

R

R

A

T

P

P

A

D

G

G

V

V

D

D

G

E

Q

K

D

A

V

L

V

R

K

Q

L

A

R

R

D

A

K

R

Y

Y

G

G

I

V

M

V

I

F

A

S

G

S

G

G

Q

R

A

G

E

E

A

T

K

L

G

G

K

K

I

L

F

T

R
|
R

I

I

A

G

H

H

M

M

G

G

Y

P

I

T

G

A

N

Q

F

-

D

-

I

P

V

I

M

Y

I

A

I

I

A

A

L

L

E

T

V

A

V

L

-

G

-

A

L

M

N

N

E

A

L

A

G

G

Y

R

K

K

A

L

P

A

Y

I

G

G

A

K

G

G

V

I

K

E

A

A

E

L

E

A

V

V

L

I

binding alanine: Y94 (≠ F88), R344 (= R334)
binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ T61), Y94 (≠ F88), T145 (= T139), D170 (= D165), V172 (≠ I167), K196 (= K191)

Query Sequence

>WP_013706246.1 NCBI__GCF_000195295.1:WP_013706246.1
MKKHYLLAPGPTPVSPETLLAMATPIIHHRSPQFAEVVAECRVGLKYLFQTKQEVLILAS
TGTGAMEGAITNTLSPGDTALVVRGGKFGERWGEICAAYGVNFEAIDVEWGRAVQVADVA
AKLKANPAIKAVCIQAHETSTGVNHPVKELAELTKSLPGTLLLVDAISALGAFELPMDAW
GIDIMVAGSQKAMMLPPGLAFACLSEKAWEFTKTATCNKYYFNFSKELKNIQKNTGAYTS
AVSLVMGLRDVLRYFKEATLEKIFAEHQLMSKATKAAVKALGLELFSQEGASDALTAVRA
PAGVDGQDVVKLLRDKYGIMIAGGQAEAKGKIFRIAHMGYIGNFDIVMIIAALEVVLNEL
GYKAPYGAGVKAAEEVLFGGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory