SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013707831.1 NCBI__GCF_000195295.1:WP_013707831.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
55% identity, 100% coverage: 1:339/339 of query aligns to 1:337/337 of P23247

query
sites
P23247

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C132 (= C132) active site, Acyl-thioester intermediate

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
55% identity, 100% coverage: 2:339/339 of query aligns to 1:336/336 of 2r00C

query
sites
2r00C

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active site: C131 (= C132), Q158 (= Q159), R237 (= R238), H244 (= H245)
binding 2,2'-oxydiacetic acid: V15 (= V16), N95 (= N96), C131 (= C132), N315 (= N318)

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
55% identity, 99% coverage: 1:334/339 of query aligns to 1:340/346 of Q04797

query
sites
Q04797

M

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S98 (= S98) modified: Phosphoserine
Y146 (≠ A148) modified: Phosphotyrosine

4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/360 of 4r51A

query
sites
4r51A

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P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), N93 (= N96), T94 (≠ S97), N126 (= N131), C127 (= C132), G160 (= G165), M161 (≠ Q166), G328 (= G322)
binding phthalic acid: S73 (≠ G76), T94 (≠ S97), S95 (= S98), R98 (= R101), N126 (= N131), K222 (= K216)

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/359 of 4r5hA

query
sites
4r5hA

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V
x
T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G76), T94 (≠ S97), S95 (= S98), R98 (= R101), N126 (= N131), C127 (= C132), Q154 (= Q159), G158 (= G163), K222 (= K216), R244 (= R238), H251 (= H245)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (≠ V78), N93 (= N96), T94 (≠ S97), P125 (= P130), N126 (= N131), C127 (= C132), G160 (= G165), M161 (≠ Q166), G328 (= G322)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/359 of 4r4jA

query
sites
4r4jA

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

V
x
T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S97), S95 (= S98), R98 (= R101), N126 (= N131), C127 (= C132), Q154 (= Q159), G158 (= G163), E219 (= E213), K222 (= K216), R244 (= R238), H251 (= H245)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (≠ V78), N93 (= N96), T94 (≠ S97), N126 (= N131), C127 (= C132), G160 (= G165), M161 (≠ Q166), G328 (= G322)

3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/359 of 3pyxB

query
sites
3pyxB

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

V
x
T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S
|
S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N
|
N

I
x
L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R101), G158 (= G163), E219 (= E213), K222 (= K216), R244 (= R238)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (≠ V78), C127 (= C132), S157 (= S162), G158 (= G163), G160 (= G165), M161 (≠ Q166), N324 (= N318), L325 (≠ I319)

3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/361 of 3pylC

query
sites
3pylC

F

Y

N

T

V

V

A

A

V

V

A

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A

A

S

S

E

A

R

R

S

S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L

T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

S

S

V

T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

T

A

G

G

Q

M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding 3-amino-D-alanine: N126 (= N131), C127 (= C132), G158 (= G163), E219 (= E213), R244 (= R238)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/357 of 4r54A

query
sites
4r54A

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V
x
T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q

M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R

R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G75), S73 (≠ G76), T94 (≠ S97), S95 (= S98), R98 (= R101), K222 (= K216)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (≠ V78), N93 (= N96), T94 (≠ S97), N126 (= N131), C127 (= C132), G160 (= G165), G328 (= G322)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/357 of 4r41A

query
sites
4r41A

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V

T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q
x
M

K
x
G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R

R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S73), G72 (= G75), S73 (≠ G76), N93 (= N96), T94 (≠ S97), S95 (= S98), R98 (= R101), K222 (= K216)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), A71 (= A74), G160 (= G165), M161 (≠ Q166), G162 (≠ K167)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/357 of 4r3nA

query
sites
4r3nA

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R

R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V

T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q
x
M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R238), H251 (= H245)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G76), T94 (≠ S97), S95 (= S98), R98 (= R101), N126 (= N131), K222 (= K216)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), N93 (= N96), T94 (≠ S97), N126 (= N131), C127 (= C132), G160 (= G165), M161 (≠ Q166), G328 (= G322)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/357 of 3q1lA

query
sites
3q1lA

F

Y

N

T

V

V

A

A

V

V

A

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A

A

S

S

E

A

R

R

S

S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L

T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

S

S

V

T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G

G

Q

M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G

-

V

-

K

-

P

-

C

E

D

A

L

E

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

Y

Y

R

P

I

I

A

A

F

F

N

N

V

A

F

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

E

D

N

N

G

D

F

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

K

K

I

I

M

M

G

E

D

D

S

S

I

I

R

A

V

V

C

S

A

A

T

T

T

C

A

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

V

V

N

Y

I

I

E

E

T

T

E

K

R

E

K

V

I

A

S

P

P

I

D

E

V

V

R

K

A

A

L

A

L

I

A

A

Q

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

R

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

Q

K

D

D

T

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

I

T

L

L

A

H

E

E

K

R

Y

G

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R238), H251 (= H245)
binding 2-amino-ethanethiol: N126 (= N131), C127 (= C132), G158 (= G163), E219 (= E213)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
52% identity, 99% coverage: 5:339/339 of query aligns to 2:345/357 of 3pwsA

query
sites
3pwsA

F

Y

N

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

T

A

Q

Q

M

M

V

I

Q

K

C

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

E

D

L

K

L

I

R

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

L

A

G

G

K

K

S

S

V

L

S

K

F

F

H

K

G

D

Q

Q

E

D

I

I

P

T

V

I

E

E

V

E

L
x
T

N

T

E

E

N

T

S

A

F

F

V

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

S

S

V
x
T

S

S

L

A

K

K

Y

Y

G

A

P

P

I

Y

A

A

A

V

A

K

S

A

G

G

A

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

I

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

D

H

D

-

I

-

A

A

L

L

Y

D

K

A

N

H

R

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

I

V

H

R

D

Q

A

K

A

W

R

G

L

L

K

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

V

I

T

L

E

E

L

T

A

Q

A

R

Q

E

V

L

R

R

A

E

L

V

L

L

S

N

G

D

Q

G