SitesBLAST
Comparing WP_013723230.1 NCBI__GCF_000204645.1:WP_013723230.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
50% identity, 79% coverage: 3:300/377 of query aligns to 1:303/378 of P69874
- C26 (≠ N28) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F29) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ M47) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C56) mutation to T: Loss of ATPase activity and transport.
- L60 (= L62) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ Q78) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ A137) mutation to M: Loss of ATPase activity and transport.
- D172 (= D174) mutation to N: Loss of ATPase activity and transport.
- C276 (= C275) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E295) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
51% identity, 75% coverage: 18:300/377 of query aligns to 1:288/358 of 8y5iA
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
39% identity, 94% coverage: 24:377/377 of query aligns to 11:373/375 of 2d62A
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
40% identity, 95% coverage: 20:377/377 of query aligns to 7:351/353 of 1vciA
1g291 Malk (see paper)
44% identity, 72% coverage: 25:296/377 of query aligns to 9:293/372 of 1g291
- binding magnesium ion: D69 (vs. gap), E71 (vs. gap), K72 (vs. gap), K79 (≠ S89), D80 (≠ E90), E292 (= E295), D293 (= D296)
- binding pyrophosphate 2-: S38 (= S54), G39 (= G55), C40 (= C56), G41 (= G57), K42 (= K58), T43 (= T59), T44 (= T60)
Sites not aligning to the query:
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
49% identity, 60% coverage: 25:251/377 of query aligns to 9:236/393 of P9WQI3
- H193 (= H208) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 73% coverage: 19:295/377 of query aligns to 2:287/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 73% coverage: 19:295/377 of query aligns to 2:287/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F29), S37 (= S54), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (≠ T59), T43 (= T60), Q81 (= Q98), R128 (≠ K145), A132 (≠ Q149), S134 (= S151), G136 (= G153), Q137 (= Q154), E158 (= E175), H191 (= H208)
- binding magnesium ion: S42 (≠ T59), Q81 (= Q98)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 73% coverage: 19:295/377 of query aligns to 2:287/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F29), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (≠ T59), T43 (= T60), R128 (≠ K145), S134 (= S151), Q137 (= Q154)
- binding beryllium trifluoride ion: S37 (= S54), G38 (= G55), K41 (= K58), Q81 (= Q98), S134 (= S151), G136 (= G153), H191 (= H208)
- binding magnesium ion: S42 (≠ T59), Q81 (= Q98)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 73% coverage: 19:295/377 of query aligns to 2:287/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F29), V17 (≠ A34), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (≠ T59), T43 (= T60), R128 (≠ K145), A132 (≠ Q149), S134 (= S151), Q137 (= Q154)
- binding tetrafluoroaluminate ion: S37 (= S54), G38 (= G55), K41 (= K58), Q81 (= Q98), S134 (= S151), G135 (= G152), G136 (= G153), E158 (= E175), H191 (= H208)
- binding magnesium ion: S42 (≠ T59), Q81 (= Q98)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 73% coverage: 19:295/377 of query aligns to 2:287/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F29), V17 (≠ A34), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (≠ T59), T43 (= T60), R128 (≠ K145), A132 (≠ Q149), S134 (= S151), Q137 (= Q154)
- binding magnesium ion: S42 (≠ T59), Q81 (= Q98)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 73% coverage: 19:295/377 of query aligns to 3:288/371 of P68187
- A85 (= A101) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ R122) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V130) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L133) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A135) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ P140) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G153) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D174) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ P244) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ V255) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ E274) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G285) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (= S289) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ Y291) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 73% coverage: 20:295/377 of query aligns to 1:285/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F29), S35 (= S54), G36 (= G55), C37 (= C56), G38 (= G57), K39 (= K58), S40 (≠ T59), T41 (= T60), R126 (≠ K145), A130 (≠ Q149), S132 (= S151), G134 (= G153), Q135 (= Q154)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
42% identity, 73% coverage: 19:295/377 of query aligns to 3:286/369 of P19566
- L86 (= L102) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P176) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D181) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
8hplC Lpqy-sugabc in state 1 (see paper)
48% identity, 58% coverage: 33:251/377 of query aligns to 15:233/384 of 8hplC
Sites not aligning to the query:
8hprC Lpqy-sugabc in state 4 (see paper)
48% identity, 58% coverage: 33:251/377 of query aligns to 17:235/363 of 8hprC