SitesBLAST

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
35% identity, 72% coverage: 45:175/181 of query aligns to 56:190/209 of P50870

query
sites
P50870

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H82 (≠ V74) mutation to A: 105-fold decrease in activity.

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
35% identity, 72% coverage: 45:175/181 of query aligns to 56:190/206 of 1khrA

query
sites
1khrA

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binding coenzyme a: H82 (≠ V74), M84 (≠ I76), W121 (≠ L108), A141 (= A128), A142 (≠ S129), V147 (≠ T134), L155 (≠ V142), G157 (= G144), P160 (= P147), I164 (= I151)
binding virginiamycin m1: A80 (≠ S72), N81 (≠ Y73), H82 (≠ V74), L93 (≠ K85)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
62% identity, 32% coverage: 98:155/181 of query aligns to 109:166/210 of 6pubA

query
sites
6pubA

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Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
62% identity, 32% coverage: 98:155/181 of query aligns to 106:163/206 of 6u9cA

query
sites
6u9cA

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binding acetyl coenzyme *a: W116 (≠ L108), A136 (= A128), I159 (= I151)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
34% identity, 73% coverage: 28:160/181 of query aligns to 72:190/200 of 1krrA

query
sites
1krrA

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binding acetyl coenzyme *a: N84 (≠ R40), I103 (= I58), A104 (= A59), P105 (= P60), T112 (= T77), H114 (≠ S79), G140 (= G110), S141 (≠ H111), N146 (≠ M116), G158 (≠ A128), A159 (≠ S129), A174 (≠ G144), P177 (= P147), R182 (≠ K152)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
34% identity, 73% coverage: 28:160/181 of query aligns to 72:190/201 of 1krvA

query
sites
1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ I38), N84 (≠ R40), V90 (≠ F46), D92 (= D48), M126 (≠ P96)
binding coenzyme a: A104 (= A59), S110 (≠ A75), T112 (= T77), W138 (≠ L108), G140 (= G110), S141 (≠ H111), N146 (≠ M116), A159 (≠ S129), P177 (= P147), R179 (≠ K149), R182 (≠ K152)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
34% identity, 73% coverage: 28:160/181 of query aligns to 72:190/201 of 1kruA

query
sites
1kruA

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binding coenzyme a: H114 (≠ S79), W138 (≠ L108), G140 (= G110), S141 (≠ H111), G158 (≠ A128), A159 (≠ S129), P177 (= P147), R182 (≠ K152)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ I38), Y82 (≠ I38), N84 (≠ R40), V90 (≠ F46), D92 (= D48), L102 (≠ E57), H114 (≠ S79), M126 (≠ P96)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
33% identity, 71% coverage: 42:169/181 of query aligns to 53:178/208 of 2xatA

query
sites
2xatA

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binding desulfo-coenzyme a: G118 (= G110), T119 (≠ H111), G136 (≠ A128), S137 (= S129), G152 (= G144), G153 (= G145), I159 (= I151)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 73% coverage: 28:160/181 of query aligns to 73:191/203 of P07464

query
sites
P07464

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N85 (≠ R40) binding in other chain; binding in other chain
D93 (= D48) binding
H115 (≠ S79) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ H111) binding in other chain
A160 (≠ S129) binding in other chain
TK 165:166 (= TK 134:135) binding
R180 (≠ K149) binding
R183 (≠ K152) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
32% identity, 78% coverage: 24:164/181 of query aligns to 32:166/191 of G3XD01

query
sites
G3XD01

G

G

T

A

T

R

A

I

G

G

K

A

G

G

F

V

H

S

V

L

G

G

K

Q

N

N

V

V

I

F

I

V

R

-

G

G

N

N

N

K

C

V

V

V

F

I

G

G

D

D

Y

R

V

C

F

K

I

I

G

Q

E

N

N

N

S

V

E

S

I

V

A

Y

P

D

A

N

V

V

T

T

I

L

G

E

N

E

Y

G

A

V

M

F

I

C

S

G

S

P

Y

S

V

M

A

V

I

F

T

T

G

-

S

-

D

-

H

N

I

V

Y

Y

N

N

K

P

P

R

G

S

V

L

A

I

I

E

R

R

F

-

S

-

G

-

R

K

P

D

D

Q

S

Y

V

R

K

N

T

T

H

L

I

V

G

K

H

K

D

G

V

A

L

T

I

L

G

G

H

A

G

N

A

C

I

T

V

I

M

V

R

C

G

G

V

V

T

T

I

I

G

G

N

E

G

Y

A

A

V

F

I

V

A

G

S

A

G

G

A

A

V

V

V

I

T

N

K
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K

D

N

V

V

P

P

A

S

Y

Y

A

A

V

L

M

M

G

V

G

G

V

V

P

P

A

A

K

R

F

Q

I

I

K

G

W

W

R

M

F

S

D

E

H

F

-

G

E

E

Q

Q

Q

L

K

Q

L

L

H

N

E

E

K136 (= K135) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
36% identity, 61% coverage: 45:155/181 of query aligns to 55:167/211 of 4hurA

query
sites
4hurA

V

V

F

I

G

G

D

D

Y

K

V

L

F

I

I

I

G

G

E

R

N

F

S

C

E
x
S

I
|
I

A
x
G

P

P

A

G

V

T

T

T

I

-

G

-

N

-

Y

F

A

I

M

M

I

N

S

G

S
x
A

Y
x
N

V

H

A

R

I

M

T

D

G

G

S

S

D

T

H

Y

I

P

Y

F

N

H

K

L

P

F

G

R

V

M

A

G

I

W

R

E

-

K

-

Y

-

M

-

P

-

S

F

L

S

K

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D

R

L

P

P

D

L

S
x
K

V

G

K

D

T

I

H

E

I

I

G

G

H

N

D

D

V

V

L
x
W

I
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I

G
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G

H
x
R

G

D

A

V

I

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V

I

M
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M

R

P

G

G

V

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

I

I

A
|
A

S
x
A

G

E

A

A

V

V

T
|
T

K

K

D

N

V

V

P

A

A

P

Y

Y

A

S

V

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M

V

G
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G

G

G

V

N

P
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P

A

L

K

K

F

F

I
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I

K
x
R

W

K

R

R

F

F

binding acetyl coenzyme *a: S67 (≠ E57), I68 (= I58), G69 (≠ A59), A79 (≠ S72), N80 (≠ Y73), K110 (≠ S98), W120 (≠ L108), I121 (= I109), G122 (= G110), R123 (≠ H111), M128 (= M116), A140 (= A128), A141 (≠ S129), T146 (= T134), G156 (= G144), P159 (= P147), I163 (= I151), R164 (≠ K152)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
36% identity, 61% coverage: 45:155/181 of query aligns to 55:167/212 of 4husA

query
sites
4husA

V

V

F

I

G

G

D

D

Y

K

V

L

F

I

I

I

G

G

E

R

N

F

S

C

E

S

I

I

A

G

P

P

A

G

V

T

T

T

I

-

G

-

N

-

Y

F

A

I

M

M

I

N

S

G

S

A

Y

N

V

H

A

R

I

M

T

D

G

G

S

S

D

T

H

Y

I

P

Y

F

N

H

K

L

P

F

G

R

V

M

A

G

I

W

R

E

-

K

-

Y

-

M

-

P

-

S

F

L

S

K

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D

R

L

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P

D

L

S

K

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I

I

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G

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N

D

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V

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G

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R

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M

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V

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K

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D

G

G

A

A

V

I

I

I

A

A

S

A

G

E

A

A

V

V

T

T

K

K

D

N

V

V

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A

A

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Y

Y

A

S

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G

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N

P

P

A

L

K

K

F

F

I

I

K

R

W

K

R

R

F

F

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
36% identity, 61% coverage: 45:155/181 of query aligns to 55:167/206 of 6x3jA

query
sites
6x3jA

V
|
V

F

I

G

G

D

D

Y

K

V

L

F

I

I

I

G

G

E

R

N

F

S

C

E

S

I

I

A
x
G

P

P

A

G

V

T

T

T

I

-

G

-

N

-

Y

F

A

I

M

M

I

N

S

G

S
x
A

Y

N

V
x
H

A

R

I

M

T

D

G

G

S

S

D

T

H

Y

I

P

Y

F

N
x
H

K
x
L

P

F

G

R

V

M

A

G

I

W

R

E

-

K

-

Y

-
x
M

-
x
P

-

S

F

L

S

K

G

D

R
x
L

P

P

D

L

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K

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K

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I

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I

I

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G

H

N

D

D

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V

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x
W

I

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G

H

R

G

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A

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I

T

V

I

M

M

R

P

G

G

V

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

I

I

A

A

S

A

G

E

A

A

V

V

T

T

K

K

D

N

V

V

P

A

A

P

Y

Y

A

S

V

I

M

V

G

G

V

N

P

P

A

L

K

K

F

F

I

I

K

R

W

K

R

R

F

F

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: V55 (= V45), H91 (≠ N84), L92 (≠ K85), M101 (vs. gap), P102 (vs. gap), L107 (≠ R95)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A59), A79 (≠ S72), H81 (≠ V74), W120 (≠ L108), G122 (= G110)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
36% identity, 61% coverage: 45:155/181 of query aligns to 55:167/207 of 6x3cA

query
sites
6x3cA

V

V

F

I

G

G

D

D

Y

K

V

L

F

I

I

I

G

G

E

R

N

F

S

C

E

S

I

I

A
x
G

P

P

A

G

V

T

T

T

I

-

G

-

N

-

Y

F

A

I

M

M

I

N

S
x
G

S

A

Y
x
N

V
x
H

A

R

I

M

T

D

G

G

S

S

D

T

H

Y

I

P

Y

F

N
x
H

K
x
L

P

F

G

R

V

M

A

G

I

W

R

E

-

K

-

Y

-
x
M

-
x
P

-

S

F
x
L

S

K

G

D

R

L

P

P

D

L

S

K

V

G

K

D

T

I

H

E

I

I

G

G

H

N

D

D

V

V

L
x
W

I

I

G
|
G

H
x
R

G

D

A

V

I

T

V

I

M
|
M

R

P

G

G

V

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

I

I

A
|
A

S
x
A

G

E

A

A

V

V

T

T

K

K

D

N

V

V

P

A

A

P

Y

Y

A

S

V

I

M

V

G

G

V

N

P

P

A

L

K

K

F

F

I

I

K

R

W

K

R

R

F

F

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (≠ S71), N80 (≠ Y73), H81 (≠ V74), H91 (≠ N84), L92 (≠ K85), M101 (vs. gap), P102 (vs. gap), L104 (≠ F92)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A59), N80 (≠ Y73), H81 (≠ V74), W120 (≠ L108), G122 (= G110), R123 (≠ H111), M128 (= M116), A140 (= A128), A141 (≠ S129)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
36% identity, 61% coverage: 45:155/181 of query aligns to 55:167/203 of 6x3cE

query
sites
6x3cE

V

V

F

I

G

G

D

D

Y

K

V

L

F

I

I

I

G

G

E

R

N

F

S

C

E

S

I

I

A
x
G

P

P

A

G

V

T

T

T

I

-

G

-

N

-

Y

F

A

I

M

M

I

N

S
x
G

S

A

Y
x
N

V
x
H

A

R

I
x
M

T

D

G

G

S

S

D

T

H

Y

I

P

Y

F

N
x
H

K
x
L

P

F

G

R

V

M

A

G

I

W

R

E

-

K

-

Y

-
x
M

-
x
P

-

S

F

L

S

K

G

D

R

L

P

P

D

L

S

K

V

G

K

D

T

I

H

E

I

I

G

G

H

N

D

D

V

V

L
x
W

I

I

G
|
G

H

R

G

D

A

V

I

T

V

I

M

M

R

P

G

G

V

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

I

I

A
|
A

S
x
A

G

E

A

A

V

V

T

T

K

K

D

N

V

V

P

A

A

P

Y

Y

A

S

V

I

M

V

G

G

V
x
N

P
|
P

A

L

K

K

F

F

I

I

K

R

W

K

R

R

F

F

binding magnesium ion: N158 (≠ V146), P159 (= P147)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (≠ S71), N80 (≠ Y73), H81 (≠ V74), H91 (≠ N84), L92 (≠ K85), M101 (vs. gap), P102 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A59), H81 (≠ V74), M83 (≠ I76), W120 (≠ L108), G122 (= G110), A140 (= A128), A141 (≠ S129)

Sites not aligning to the query:

Query Sequence

>WP_013817130.1 NCBI__GCF_000214665.1:WP_013817130.1
MKDQIKEFLKACRAKYLLITKYRGTTAGKGFHVGKNVIIRGNNCVFGDYVFIGENSEIAP
AVTIGNYAMISSYVAITGSDHIYNKPGVAIRFSGRPDSVKTHIGHDVLIGHGAIVMRGVT
IGNGAVIASGAVVTKDVPAYAVMGGVPAKFIKWRFDHEQQKLHEAMLYSPTKFINGLERP
F

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory