SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013818545.1 NCBI__GCF_000214665.1:WP_013818545.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
30% identity, 99% coverage: 1:357/361 of query aligns to 1:376/386 of P0A9J8

query
sites
P0A9J8

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R11 (= R11) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R28) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K39) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E54) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ E89) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
35% identity, 73% coverage: 94:356/361 of query aligns to 4:273/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T261), F171 (= F263)
binding phenylalanine: N204 (= N287), E205 (≠ Q288), L209 (= L292), D222 (≠ S305), L223 (≠ M306), T224 (≠ A307), K225 (≠ H308), S228 (= S311), Y238 (= Y321), F240 (= F323)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
36% identity, 73% coverage: 96:359/361 of query aligns to 5:277/306 of 3mwbA

query
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3mwbA

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active site: T176 (= T261), F178 (= F263)
binding phenylalanine: D205 (≠ N287), H206 (≠ Q288), L210 (= L292), N223 (≠ S305), L224 (≠ M306), S225 (≠ A307), R226 (≠ H308), S229 (= S311), F241 (= F323)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
35% identity, 73% coverage: 96:359/361 of query aligns to 5:274/303 of 3mwbB

query
sites
3mwbB

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active site: T176 (= T261), F178 (= F263)
binding magnesium ion: A155 (= A240), Q158 (≠ L243), L161 (= L246)
binding phenylalanine: D205 (≠ N287), H206 (≠ Q288), L210 (= L292), N223 (≠ S305), L224 (≠ M306), S225 (≠ A307), R226 (≠ H308), I227 (= I309), Y236 (= Y321), F238 (= F323)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
32% identity, 73% coverage: 94:356/361 of query aligns to 9:276/282 of 6vh5D

query
sites
6vh5D

V

I

A

S

Y

F

L

Q

G

G

P

E

Q

A

G

G

T

A

F

N

T

S

Q

D

Q

T

A

A

T

C

F

R

K

N

H

M

F

F

G

P

H

D

A

-

V

M

K

E

D

P

V

L

P

P

V

C

P

P

T

T

I

F

H

E

E

D

I

A

F

F

Q

N

A

A

V

V

E

E

T

T

G

G

H

A

C

A

Q

D

F

L

G

A

V

M

V

I

P

P

V

I

E

E

N

N

S

T

T

L

E

A

G

G

V

R

I

V

A

A

H

D

T

I

L

H

D

Y

R

L

F

L

I

P

N

L

S

A

P

D

L

M

K

H

I

I

C

V

G

G

E

E

V

Y

E

F

I

L

R

P

V

I

H

H

Q

F

N

Q

L

L

L

M

G

-

K

-

M

-

D

-

D

V

L

L

I

P

G

G

-

V

-

R

-

E

I

I

D

K

E

T

V

V

F

H

S

S

H

H

Q

I

Q

H

S

A

L

L

A

G

Q

Q

C

C

R

R

Q

N

W

V

L

I

N

R

T

Q

Y

N

L

-

P

-

G

G

V

W

T

K

V

G

T

V

A

I

V

A

S

G

S

D

N

T

A

A

E

G

A

A

R

R

L

L

A

V

S

A

-

D

-

V

L

K

D

D

H

R

S

S

K

M

A

A

I

L

A

A

G

P

L

R

V

L

A

A

E

D

L

L

Y

Y

D

G

L

L

N

D

I

I

I

L

E

E

K

E

N

N

I

V

E

E

D

D

E

S

A

E

N

N

T

V

T

T

R

R

F

F

I

V

I

L

G

S

-

K

-

N

-

K

-

Q

-

W

A

A

Q

A

Q

R

P

P

V

E

S

N

T

D

G

E

L

R

D

I

K

V

T

T

S

T

I

F

L

V

V

F

S

R

T

V

G

R

N
|
N

Q
x
V

S

P

G

A

A

A

L
|
L

H

Y

R

K

I

A

L

L

G

G

P

G

F

F

A

A

E

T

Y

N

G

G

I

V

S
x
N

M
|
M

A
x
T

H
x
K

I

L

E

E

S
|
S

R

Y

P

Q

S

L

R

G

Q

G

G

R

L

F

W

I

D

A

Y
x
T

V

Q

F
|
F

F

Y

I

A

D

D

I

I

E

E

G

G

H

H

C

P

D

E

E

R

H

S

V

V

A

Q

K

L

A

A

L

L

K

E

V

E

L

L

Q

R

N

F

V

T

K

K

M

E

L

V

K

R

I

I

L

L

G

G

S

V

Y

Y

binding phenylalanine: N207 (= N287), V208 (≠ Q288), L212 (= L292), N225 (≠ S305), M226 (= M306), T227 (≠ A307), K228 (≠ H308), S231 (= S311), T241 (≠ Y321), F243 (= F323)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
29% identity, 74% coverage: 94:359/361 of query aligns to 4:272/278 of 7am0B

query
sites
7am0B

V

I

A

A

Y

F

L

Q

G

G

P

R

Q

P

G

G

T

A

F

Y

T

S

Q

D

Q

L

A

A

T

C

F

-

K

R

H

Q

F

A

G

R

H

P

A

G

V

W

K

T

D

T

V

L

P

P

V

C

P

Q

T

T

I

F

H

A

E

Q

I

T

F

I

Q

A

A

A

V

V

E

H

T

D

G

G

H

R

C

A

Q

E

F

L

G

A

V

M

V

L

P

A

V

C

E

E

N

N

S

S

T

L

E

A

G

G

V

R

I

V

A

P

H

D

T

I

L

H

D

A

R

L

F

L

I

P

N

E

S

A

P

G

L

L

K

F

I

I

C

V

G

G

E

E

V

H

E

F

I

Q

R

R

V

V

H

E

Q

H

N

C

L

L

G

G

-

I

K

P

M

G

D

S

D

T

L

L

I

A

G

D

I

A

D

R

E

R

V

I

F

H

S

T

H

H

Q

P

Q

V

S

A

L

M

A

A

Q

Q

C

V

R

R

Q

G

W

I

L

I

N

T

T

E

Y

L

L

-

P

-

G

G

V

L

T

D

V

P

T

V

A

V

V

E

S

F

S

D

N

T

A

A

E

G

A

A

R

E

L

M

A

V

S

R

L

E

D

W

H

G

S

R

K

K

-

E

-

D

A

V

A

A

I

V

A

A

G

S

L

A

V

L

A

A

E

E

L

L

Y

N

D

G

L

L

N

E

I

I

I

L

E

R

K

R

N

N

I

V

E

E

D

D

E

A

A

T

N

H

N

N

T

T

R

R

F

F

I

Y

I

I

I

A

G

S

A

R

Q

R

-

P

-

A

-

T

-

L

Q

P

P

P

V

P

S

G

T

P

G

G

L

F

D

-

K

M

T

T

S

T

I

L

L

L

V

F

S

R

T

V

G
x
N

N
|
N

Q
|
Q

S
x
P

G

G

A

A

L
|
L

H

Y

R

K

I

A

L

L

G

G

P

G

F

L

A

A

E

T

Y

A

G

G

I

V

S
x
N

M
|
M

A
x
T

H
x
R

I

L

E

E

S

S

R

Y

P

M

S

L

R

E

Q

G

G

S

L

F

W

S

D

A

Y
x
T

V

Q

F
|
F

F

L

I

M

D

D

I

V

E

E

G

G

H

H

C

P

D

E

D

A

E

P

H

P

V

L

A

A

K

R

A

A

L

L

K

D

V

E

L

L

Q

S

N

F

V

S

K

E

M

Q

L

Q

K

E

I

I

L

L

G

G

S

V

Y

Y

P

P

K

A

A

S

binding phenylalanine: N199 (≠ G286), N200 (= N287), Q201 (= Q288), P202 (≠ S289), L205 (= L292), N218 (≠ S305), M219 (= M306), T220 (≠ A307), R221 (≠ H308), T234 (≠ Y321), F236 (= F323)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
26% identity, 73% coverage: 96:358/361 of query aligns to 8:285/326 of 3luyA

query
sites
3luyA

Y

Y

L

L

G

G

P

P

Q

Q

G

G

T

T

F

F

T

T

Q

H

Q

Q

A

A

T

A

-

V

-

N

-

A

-

Q

-

E

-

L

-

A

-

R

F

F

K

E

H

P

F

Q

G

G

H

F

A

D

V

L

K

-

D

-

V

M

P

P

V

M

P

D

T

D

I

V

H

P

E

Q

I

I

F

L

Q

D

A

A

V

A

E

Q

T

H

G

G

H

D

C

G

Q

-

F

W

G

G

V

I

V

V

P

A

V

W

E

E

N

N

S

N

T

V

E

E

G

G

V

Y

I

V

A

V

H

P

T

N

L

L

D

D

R

A

F

L

I

I

N

D

S

A

P

K

L

-

K

D

I

L

C

V

G

G

E

F

V

A

E

R

I

V

R

G

V

V

H

N

Q

V

N

E

L

F

-

D

-

A

-

Y

L

V

G

A

K

Q

M

G

D

A

D

D

L

P

I

A

G

E

I

A

D

R

E

I

V

A

F

T

S

A

H

H

Q

P

Q

H

S

G

L

L

A

A

Q

Q

C

C

R

K

Q

R

W

F

L

I

N

A

T

E

Y

H

L

R

P

-

G

-

V

L

T

S

V

T

T

Q

A

P

V

A

S

T

S

S

N

N

A

A

E

A

A

A

A

C

R

R

L

-

A

-

S

D

L

L

D

I

H

P

S

G

K

E

A

I

A

A

I

F

A

G

G

P

L

A

V

I

A

C

A

G

E

E

L

L

Y

Y

D

D

L

I

N

T

I

R

I

I

E

G

K

T

N

A

I

I

E

Q

D

D

E

Y

A

Q

N

G

N

A

T

A

T
|
T

R

D

F

F

I

L

I

V

I

L

G

S

A

P

Q

R

Q

A

P

E

V

V

S

A

T

R

G

L

L

L

D

A

K

K

-

P

-

R

-

A

-

E

-

A

-

N

-

V

-

E

-

Y

-

E

-

S

-

V

-

L

T

T

S

L

I

I

L

P

V

L

S

V

T

T

G
|
G

N

-

Q

-

S

P

G

G

A

V

L
|
L

H

A

R

N

I

L

L

L

G

D

P

V

F

F

A

R

E

D

Y

A

G

G

I

L

S

N

M

M

A

T

H

S

I

F

E

I

S

S

R

R

P

P

S

I

R

K

Q

G

G

R

L

T

W

G

D

T

Y
|
Y

V

S

F
|
F

F

I

I

V

D

T

I

L

E

D

G

A

H

A

C

P

D

W

D

E

E

E

H

R

V

F

A

R

K

D

A

A

L

L

K

V

V

E

L

I

Q

A

N

E

N

H

V

G

K

D

M

W

L

A

K

K

I

T

L

L

G

A

S

V

Y

Y

P

P

K

R

active site: T177 (= T261)
binding 3-phenylpyruvic acid: G215 (= G286), L219 (= L292), Y248 (= Y321), F250 (= F323)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
38% identity, 23% coverage: 7:90/361 of query aligns to 4:85/352 of 5j6fA

query
sites
5j6fA

L

L

S

D

E

E

L

L

R

R

T

A

Q

R

I

V

D

D

A

E

I

I

D

N

Q

L

Q

Q

I

L

L

L

Q

K

L

L

I

I

N

N

Q

E

R
|
R

A

G

N

R

C

L

A

V

I

Q

E

E

V
x
I

A

G

K

K

T

I

K
|
K

Q

E

A

A

E

Q

G

G

E

T

T

-

G

-

S

H

F

R

Y
|
Y

R
x
D

P

P

D

V

R
|
R

E

E

A

R

Q

K

V
x
M

L

L

R

D

R

L

I

I

K

S

E

E

L

H

N

N

P

D

G

G

P

P

L

F

S

E

D

T

D

S

A

T

A

L

A

Q

R

H

L

I

F

F

R

K

E

E

V

I

M

F

S

K

A

A

C

A

L

L

A

E

L

L

E

Q

K

E

binding prephenic acid: R25 (= R28), I32 (≠ V35), K36 (= K39), Y44 (= Y49), D45 (≠ R50), R48 (= R53), M52 (≠ V57)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
34% identity, 28% coverage: 258:359/361 of query aligns to 84:188/194 of 7alzA

query
sites
7alzA

N

H

N

N

T

T

R

R

F

F

I

Y

I

I

I

A

G

S

A

R

Q

R

Q

P

-

A

-

T

-

L

-

P

P

P

V

P

S

G

T

P

G

G

L

F

D

-

K

M

T

T

S

T

I

L

L

L

V

F

S

R

T

V

G

N

N

N

Q
|
Q

S

P

G

G

A

A

L
|
L

H

Y

R

K

I

A

L

L

G

G

P

G

F

L

A

A

E

T

Y

A

G

G

I

V

S
x
N

M
|
M

A
x
T

H
x
R

I

L

E

E

S

S

R

Y

P

M

S

L

R

E

Q

G

G

S

L

F

W

S

D

A

Y
x
T

V

Q

F
|
F

F

L

I

M

D

D

I

V

E

E

G

G

H

H

C

P

D

E

D

A

E

P

H

P

V

L

A

A

K

R

A

A

L

L

K

D

V

E

L

L

Q

S

N

F

V

S

K

E

M

Q

L

Q

K

E

I

I

L

L

G

G

S

V

Y

Y

P

P

K

A

A

S

binding phenylalanine: Q117 (= Q288), L121 (= L292), N134 (≠ S305), M135 (= M306), T136 (≠ A307), R137 (≠ H308), T150 (≠ Y321), F152 (= F323)

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
37% identity, 22% coverage: 10:90/361 of query aligns to 8:86/87 of 5gmuB

query
sites
5gmuB

L

L

R

R

T

Q

Q

K

I

A

D

D

A

E

I

L

D

N

Q

L

Q

Q

I

I

L

L

Q

K

L

L

I

I

N

N

Q

E

R
|
R

A

G

N

N

C

V

A

V

I

K

E

E

V

I

A

G

K

K

T

A

K

K

Q

E

A

A

E

Q

G

G

E

-

T

-

G

V

S

N

F
x
R

Y
x
F

R
x
D

P

P

D

V

R

R

E
|
E

A

R

Q

T

V

M

L

L

R

N

I

I

K

I

E

E

L

N

N

N

P

D

G

G

P

P

L

F

S

E

D

N

D

S

A

T

A

I

A

Q

R

H

L

I

F

F

R

K

E

E

V

I

M
x
F

S
x
K

A

A

C

G

L
|
L

A

E

L

L

E
x
Q

K

E

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R28), R44 (≠ F48), F45 (≠ Y49), D46 (≠ R50), E50 (= E54), F78 (≠ M82), K79 (≠ S83), L82 (= L86), Q85 (≠ E89)

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
37% identity, 23% coverage: 7:90/361 of query aligns to 6:87/358 of P39912

query
sites
P39912

L

L

S

E

E

L

L

L

R

R

T

Q

Q

K

I

A

D

D

A

E

I

L

D

N

Q

L

Q

Q

I

I

L

L

Q

K

L

L

I

I

N

N

Q

E

R

R

A

G

N

N

C

V

A

V

I

K

E

E

V

I

A

G

K

K

T

A

K

K

Q

E

A

A

E

Q

G

G

E

V

T

-

G

-

S

N

F

R

Y

F

R

D

P

P

D

V

R

R

E

E

A

R

Q

T

V

M

L

L

R

N

I

I

K

I

E

E

L

N

N

N

P

D

G

G

P

P

L

F

S

E

D

N

D

S

A

T

A

I

A

Q

R

H

L

I

F

F

R

K

E

E

V

I

M

F

S

K

A

A

C

G

L

L

A

E

L

L

E

Q

K

E

Sites not aligning to the query:

2 modified: Phosphoserine

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
39% identity, 23% coverage: 9:90/361 of query aligns to 1:75/345 of 3nvtA

query
sites
3nvtA

E

E

L

L

R

R

T

T

Q

Q

I

V

D

D

A

Q

I

L

D

N

Q

I

Q

D

I

L

L

L

Q

E

L

L

I

I

N

S

Q

K

R

R

A

A

N

N

C

L

A

V

I

Q

E

E

V

I

A

G

K

K

T

I

K

K

Q

-

A

-

E

-

G

-

E

-

T

-

G

G

S

R

F

F

Y

-

R

D

P

P

D

L

R

R

E

E

A

R

Q

E

V

M

L

L

R

N

R

T

I

I

K

L

E

A

L

A

N

N

P

E

G

G

P

P

L

F

S

E

D

D

D

S

A

T

A

V

A

Q

R

K

L

L

F

F

R

K

E

E

V

I

M

F

S

K

A

A

C

G

L

L

A

E

L

L

E

Q

K

E

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
36% identity, 22% coverage: 10:90/361 of query aligns to 1:74/343 of 3tfcA

query
sites
3tfcA

L

L

R

R

T

T

Q

Q

I

V

D

D

A

Q

I

L

D

N

Q

I

Q

D

I

L

L

L

Q

E

L

L

I

I

N

S

Q

K

R

R

A

A

N

N

C

L

A

V

I

Q

E

E

V

I

A

G

K

K

T

I

K

K

Q

L

A

R

E

-

G

-

E

-

T

-

G

-

S

-

F

-

Y

F

R

D

P

P

D

L

R

R

E

E

A

R

Q

E

V

M

L

L

R

N

R

T

I

I

K

L

E

A

L

A

N

N

P

E

G

G

P

P

L

F

S

E

D

D

D

S

A

T

A

V

A

Q

R

K

L

L

F

F

R

K

E

E

V

I

M

F

S

K

A

A

C

G

L

L

A

E

L

L

E

Q

K

E

Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

Query Sequence

>WP_013818545.1 NCBI__GCF_000214665.1:WP_013818545.1
MTNIIPLSELRTQIDAIDQQILQLINQRANCAIEVAKTKQAEGETGSFYRPDREAQVLRR
IKELNPGPLSDDAAARLFREVMSACLALEKPLEVAYLGPQGTFTQQATFKHFGHAVKDVP
VPTIHEIFQAVETGHCQFGVVPVENSTEGVIAHTLDRFINSPLKICGEVEIRVHQNLLGK
MDDLIGIDEVFSHQQSLAQCRQWLNTYLPGVTVTAVSSNAEAARLASLDHSKAAIAGLVA
AELYDLNIIEKNIEDEANNTTRFIIIGAQQPVSTGLDKTSILVSTGNQSGALHRILGPFA
EYGISMAHIESRPSRQGLWDYVFFIDIEGHCDDEHVAKALKVLQNNVKMLKILGSYPKAV
I

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory