SitesBLAST
Comparing WP_013818750.1 NCBI__GCF_000214665.1:WP_013818750.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
30% identity, 79% coverage: 96:468/474 of query aligns to 25:399/404 of 3wx9A
- binding 4-(2-aminophenyl)-2,4-dioxobutanoic acid: G40 (≠ A114), L41 (≠ V115)
- binding 2-oxoglutaric acid: D213 (= D283), P214 (≠ I284), Y215 (= Y285), G216 (= G286), E217 (≠ D287), G241 (≠ S311), T242 (≠ S312), I246 (≠ S316)
- binding (2E)-pent-2-enedioic acid: G40 (≠ A114), Y130 (≠ F204), N184 (= N254), R376 (= R445)
- binding glutamic acid: L131 (≠ Y205), V360 (≠ A429), A364 (≠ I433), R369 (≠ Q438)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G178), S105 (≠ C179), Q106 (= Q180), Y130 (≠ F204), N184 (= N254), D212 (= D282), P214 (≠ I284), Y215 (= Y285), T242 (≠ S312), S244 (= S314), K245 (= K315), R252 (= R322)
Sites not aligning to the query:
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
30% identity, 79% coverage: 96:468/474 of query aligns to 25:399/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: L131 (≠ Y205), Q135 (= Q209), A364 (≠ I433), R369 (≠ Q438)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: G40 (≠ A114), Y130 (≠ F204), L131 (≠ Y205), A132 (≠ G206), N184 (= N254), R376 (= R445)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G178), S105 (≠ C179), Q106 (= Q180), Y130 (≠ F204), V179 (= V249), N184 (= N254), D212 (= D282), P214 (≠ I284), Y215 (= Y285), T242 (≠ S312), S244 (= S314), K245 (= K315), R252 (= R322)
Sites not aligning to the query:
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
30% identity, 79% coverage: 96:468/474 of query aligns to 25:399/404 of 3aowC
- binding 2-oxoglutaric acid: Y70 (= Y144), Y130 (≠ F204), L275 (= L344)
- binding pyridoxal-5'-phosphate: G104 (= G178), S105 (≠ C179), Q106 (= Q180), Y130 (≠ F204), V179 (= V249), N184 (= N254), D212 (= D282), P214 (≠ I284), Y215 (= Y285), T242 (≠ S312), S244 (= S314), K245 (= K315), R252 (= R322)
Sites not aligning to the query:
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
30% identity, 79% coverage: 96:468/474 of query aligns to 25:399/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G178), S105 (≠ C179), Q106 (= Q180), Y130 (≠ F204), V179 (= V249), N184 (= N254), D212 (= D282), P214 (≠ I284), Y215 (= Y285), T242 (≠ S312), S244 (= S314), K245 (= K315), R252 (= R322)
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
27% identity, 78% coverage: 96:467/474 of query aligns to 22:396/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G178), S103 (≠ C179), Q104 (= Q180), Y128 (≠ F204), V177 (= V249), N182 (= N254), D210 (= D282), P212 (≠ I284), Y213 (= Y285), T240 (≠ S312), S242 (= S314), K243 (= K315), R250 (= R322)
2zc0A Crystal structure of an archaeal alanine:glyoxylate aminotransferase (see paper)
28% identity, 72% coverage: 107:449/474 of query aligns to 35:383/405 of 2zc0A
- active site: Y132 (≠ F204), D214 (= D282), A216 (≠ I284), S246 (= S314)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G106 (= G178), G107 (≠ C179), T108 (≠ Q180), Y132 (≠ F204), N186 (= N254), D214 (= D282), A216 (≠ I284), Y217 (= Y285), T244 (≠ S312), S246 (= S314), K247 (= K315), R254 (= R322)
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
29% identity, 78% coverage: 99:470/474 of query aligns to 21:389/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: G35 (≠ A113), G36 (≠ A114), G95 (= G178), S96 (≠ C179), Q97 (= Q180), Y121 (≠ F204), N170 (= N254), D198 (= D282), Y201 (= Y285), S231 (= S312), S233 (= S314), K234 (= K315), R241 (= R322), R364 (= R445)
Sites not aligning to the query:
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
29% identity, 78% coverage: 99:470/474 of query aligns to 21:389/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G178), S96 (≠ C179), Q97 (= Q180), Y121 (≠ F204), N170 (= N254), D198 (= D282), A200 (≠ I284), Y201 (= Y285), S231 (= S312), S233 (= S314), K234 (= K315), R241 (= R322)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
29% identity, 78% coverage: 99:470/474 of query aligns to 25:393/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: G39 (≠ A113), G40 (≠ A114), G99 (= G178), S100 (≠ C179), Q101 (= Q180), Y125 (≠ F204), N174 (= N254), D202 (= D282), Y205 (= Y285), S235 (= S312), S237 (= S314), K238 (= K315), R245 (= R322), R368 (= R445)
Sites not aligning to the query:
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
29% identity, 78% coverage: 99:470/474 of query aligns to 25:393/397 of Q72LL6
- G40 (≠ A114) binding substrate
- Y70 (= Y144) binding pyridoxal 5'-phosphate
- N174 (= N254) binding pyridoxal 5'-phosphate; binding substrate
- R245 (= R322) binding pyridoxal 5'-phosphate
- R368 (= R445) binding substrate
Sites not aligning to the query:
- 20 S→E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- 23 R→A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; R→Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
29% identity, 76% coverage: 109:470/474 of query aligns to 27:385/389 of 2z1yA
- binding leucine: G32 (≠ A114), Y117 (≠ F204), R360 (= R445)
- binding pyridoxal-5'-phosphate: G91 (= G178), S92 (≠ C179), Q93 (= Q180), Y117 (≠ F204), N166 (= N254), D194 (= D282), Y197 (= Y285), S227 (= S312), S229 (= S314), K230 (= K315), R237 (= R322)
8tn3A Structure of s. Hygroscopicus aminotransferase mppq complexed with pyridoxamine 5'-phosphate (pmp) (see paper)
31% identity, 70% coverage: 109:438/474 of query aligns to 22:360/388 of 8tn3A
2vgzA Crystal structure of human kynurenine aminotransferase ii (see paper)
26% identity, 78% coverage: 98:467/474 of query aligns to 7:410/414 of 2vgzA
2xh1A Crystal structure of human kat ii-inhibitor complex (see paper)
26% identity, 78% coverage: 98:467/474 of query aligns to 5:408/412 of 2xh1A
- binding (3s)-10-(4-aminopiperazin-1-yl)-9-fluoro-7-hydroxy-3-methyl-2,3-dihydro-8h-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylate: G26 (≠ A114), L27 (≠ V115), Y61 (= Y144), L280 (= L344)
- binding pyridoxal-5'-phosphate: S104 (≠ C179), Q105 (= Q180), Y129 (≠ F204), D217 (= D282), P219 (≠ I284), S247 (= S312), S249 (= S314), R257 (= R322)
3ue8A Kynurenine aminotransferase ii inhibitors (see paper)
26% identity, 78% coverage: 98:467/474 of query aligns to 5:404/410 of 3ue8A
- binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G22 (≠ A114), Y57 (= Y144), S100 (≠ C179), Q101 (= Q180), Y125 (≠ F204), N185 (= N254), D213 (= D282), P215 (≠ I284), Y216 (= Y285), S243 (= S312), S245 (= S314), K246 (= K315), R253 (= R322), R382 (= R445)
1vp4A Crystal structure of a putative aminotransferase (tm1131) from thermotoga maritima msb8 at 1.82 a resolution
24% identity, 79% coverage: 95:467/474 of query aligns to 27:410/420 of 1vp4A
- binding pyridoxal-5'-phosphate: G112 (= G178), S113 (≠ C179), Q114 (= Q180), Y138 (≠ F204), N194 (= N254), D222 (= D282), P224 (≠ I284), Y225 (= Y285), T252 (≠ S312), S254 (= S314), K255 (= K315), R262 (= R322)
4ge4A Kynurenine aminotransferase ii inhibitors (see paper)
26% identity, 76% coverage: 108:467/474 of query aligns to 29:415/422 of 4ge4A
- binding (5-hydroxy-4-{[(1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G34 (≠ A113), G35 (≠ A114), L36 (≠ V115), Y68 (= Y144), G110 (= G178), S111 (≠ C179), Q112 (= Q180), Y136 (≠ F204), N196 (= N254), D224 (= D282), P226 (≠ I284), Y227 (= Y285), S254 (= S312), S256 (= S314), K257 (= K315), R264 (= R322), R393 (= R445)
Sites not aligning to the query:
4gebA Kynurenine aminotransferase ii inhibitors (see paper)
28% identity, 62% coverage: 172:467/474 of query aligns to 110:421/428 of 4gebA
- binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ C179), Q118 (= Q180), Y142 (≠ F204), N202 (= N254), D230 (= D282), P232 (≠ I284), Y233 (= Y285), S260 (= S312), S262 (= S314), K263 (= K315), R270 (= R322), R399 (= R445)
Sites not aligning to the query:
4ge9A Kynurenine aminotransferase ii inhibitors (see paper)
28% identity, 62% coverage: 172:467/474 of query aligns to 110:421/428 of 4ge9A
- binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ C179), Q118 (= Q180), Y142 (≠ F204), N202 (= N254), D230 (= D282), P232 (≠ I284), Y233 (= Y285), S260 (= S312), S262 (= S314), R270 (= R322), L293 (= L344), R399 (= R445)
Sites not aligning to the query:
4ge7A Kynurenine aminotransferase ii inhibitors (see paper)
28% identity, 62% coverage: 172:467/474 of query aligns to 110:421/428 of 4ge7A
- binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-6-phenoxy-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ C179), Q118 (= Q180), Y142 (≠ F204), N202 (= N254), D230 (= D282), P232 (≠ I284), Y233 (= Y285), S260 (= S312), S262 (= S314), R270 (= R322), L293 (= L344), R399 (= R445)
Sites not aligning to the query:
Query Sequence
>WP_013818750.1 NCBI__GCF_000214665.1:WP_013818750.1
MPLRYETVAEHLLTAITQGVYRPGERLPSVRLLSRQHQVSTATAVSALRLLEDQGQIEAR
QRSGYYVRPRLRSALQEPAISSPSREPTQVTGQELVLRLVTAANNPRFIQLGAAVPAPSF
LPTQKLAQLSAITARRYRQRIASYEFPPGAPELRRQIAKRMSEQGCPVDPNDILISNGCQ
EALTLALRAITAPGDIVAIESPTFYGLLQVIESLSLRAIEIPTHPREGIALDALQLACEQ
WPIKACIAVPNYSNPLGYCMSDARKQALIELAKRYRIMLIEDDIYGDLGFGPQRPSTLKS
WDKEGRVLYCSSFSKSLCPGLRVGWLVPGAFQEKIEYLKYVTNLATPTLAQLTVAEVLEH
GGYDRYLRQARQQYRQAVERMTAALGVYFPTGTRITQPEGGFLLWVELPGTVDAIALTQE
ALTLGISIAPGPIFSATQKYQHFIRLNCAVDWDERLNKALVTLGQLVHAMNINA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory