SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013819023.1 NCBI__GCF_000214665.1:WP_013819023.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
67% identity, 98% coverage: 5:294/296 of query aligns to 3:292/303 of P16703

query
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P16703

T

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N71 (= N73) binding pyridoxal 5'-phosphate
S255 (= S257) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
66% identity, 98% coverage: 5:294/296 of query aligns to 3:292/294 of 2bhtA

query
sites
2bhtA

T

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active site: K41 (= K43), S69 (= S71), Q199 (= Q201), G203 (≠ A205), S255 (= S257), C280 (= C282)
binding pyridoxal-5'-phosphate: K41 (= K43), N71 (= N73), M173 (= M175), G174 (= G176), T175 (= T177), T176 (= T178), T178 (= T180), G208 (= G210), S255 (= S257), C280 (= C282)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
44% identity, 99% coverage: 2:294/296 of query aligns to 4:306/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (= M121), Q144 (= Q142), R218 (≠ A205), H219 (≠ S206), Q222 (≠ P209), G223 (= G210), A226 (≠ R216)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
45% identity, 98% coverage: 6:294/296 of query aligns to 9:308/322 of P47998

query
sites
P47998

V

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K

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K

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K

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K

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K

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K46 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T70) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S71) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N73) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T74) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q142) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H152) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G157) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 176:180) binding pyridoxal 5'-phosphate
T182 (= T177) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T180) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R212) mutation to A: Impaired interaction with SAT1.
H221 (≠ R216) mutation to A: Impaired interaction with SAT1.
K222 (≠ E217) mutation to A: Impaired interaction with SAT1.
S269 (= S257) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
45% identity, 98% coverage: 6:294/296 of query aligns to 7:306/320 of 2isqA

query
sites
2isqA

V

V

E

T

S

E

Q

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

N

L

V

P

A

G

E

D

G

T

C

S

V

N

G

T

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

K

S

H

D

A

A

E

E

A

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

R

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

K

K

G

G

Y

Y

K

K

M

L

T

I

L

I

I

T

M

M

P

P

D

A

N

S

M

M

S

S

A

T

E

E

R

R

I

R

A

I

S

I

M

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

I

V

L

L

T

T

-

D

P

P

A

A

Q

K

G
|
G

S

-

M
|
M

E
x
K

A

G

A

A

I

I

D

A

L

K

A

A

R

E

G

E

-

I

M

L

E

A

E

K

D

T

G

P

Q

N

G

G

R

Y

I

M

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

Q

G

G

G

T

K

I

I

T

D

H

G

F

F

V

V

S

S

A
x
G

M

I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

A

A

S

N

V

V

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

E

V

G

E

A

S

S

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W
x
G

P
|
P

R
x
H

E

K

-

I

-
x
Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

D

N

A

V

Y

D

R

L

V

I

D

D

Q

E

I

V

E

Q

V

V

N

S

Q

S

T

D

D

E

A

S

E

I

D

D

T

M

T

A

R

R

A

Q

L

L

A

A

A

L

Q

K

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

F

A

A

A

L

I

Q

K

L

L

A

A

K

Q

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

F

V

V

A

A

I

I

I

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

active site: K44 (= K43), S267 (= S257)
binding pyridoxal-5'-phosphate: K44 (= K43), N75 (= N73), G177 (≠ A174), G179 (= G176), T180 (= T177), G181 (≠ T178), T183 (= T180), G223 (vs. gap), S267 (= S257), P294 (≠ C282)
binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), G122 (= G119), M123 (= M121), K124 (≠ E122), G217 (≠ W214), P218 (= P215), H219 (≠ R216), Q222 (vs. gap), G223 (vs. gap)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
42% identity, 99% coverage: 2:294/296 of query aligns to 9:311/329 of 8b9wA

query
sites
8b9wA

I

V

F

A

P

P

T

S

V

V

E

D

S

A

Q

L

V

I

G

G

N

N

T

T

P

P

L

A

V

V

R

Y

L

L

Q

R

R

N

L

M

P

-

G

N

D

K

T

T

S

A

N

A

T

T

I

I

L

V

A

L

K

K

L

L

E

E

G

S

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

S

A

M

I

I

Y

K

D

H

K

A

A

E

E

A

K

R

E

G

G

E

K

I

I

K

K

P

R

G

G

D

K

R

S

L

V

I

I

-

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

L

A

L

A

G

A

A

I

V

K

R

G

G

Y

Y

K

K

M

V

T

I

L

I

I

T

M

M

P

P

D

D

N

S

M

M

S

S

A

M

E

E

R

R

I

R

A

S

S

L

M

L

K

R

A

I

Y

F

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

A

A

Q

S

G

L

S

G

M

M

E

K

A

G

A

A

I

V

D

A

L

M

A

A

R

N

G

R

M

I

E

V

E

S

D

N

G

N

Q

P

G

D

R

A

I

V

L

L

-

A

D

D

Q

Q

F

F

A

C

N

T

P

K

D

Y

N

N

P

A

R

Q

A

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

Q

K

G

G

T

H

I

V

T

D

H

C

F

F

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

L

M

T

G

G

T

V

S

A

R

R

Y

F

L

L

K

K

E

S

Q

V

N

G

A

C

S

N

V

A

Q

N

I

I

V

V

G

A

V

V

Q

E

P

P

E

Q

G

E

A

S

S

P

K

V

I

L

P

S

G

G

I

G

R

R

R

P

W

G

P

P

R

H

E

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

E

I

V

Y

L

D

D

A

R

Y

E

R

L

V

V

D

D

Q

E

I

I

E

Q

V

V

N

S

Q

S

T

D

D

K

A

A

E

I

D

E

T

T

T

A

R

Q

A

K

L

L

A

S

A

R

Q

M

E

D

G

G

I

I

F

F

A

C

G

G

V

F

S
|
S

S

A

G

G

G

A

A

N

V

V

F

F

A

A

L

L

Q

K

L

I

A

A

K

E

Q

R

V

P

E

E

N

M

A

A

-

G

-

K

I

T

I

I

V

V

A

T

I

V

I

I

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

Y

binding pyridoxal-5'-phosphate: K49 (= K43), N80 (= N73), G184 (= G176), T185 (= T177), G186 (≠ T178), T188 (= T180), G228 (vs. gap), S272 (= S257), P299 (≠ C282)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
45% identity, 98% coverage: 6:294/296 of query aligns to 7:306/320 of 1z7yA

query
sites
1z7yA

V

V

E

T

S

E

Q

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

N

L

V

P

A

G

E

D

G

T

C

S

V

N

G

T

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

K

S

H

D

A

A

E

E

A

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

R

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

K

K

G

G

Y

Y

K

K

M

L

T

I

L

I

I

T

M

M

P

P

D

A

N

S

M

M

S

S

A

T

E

E

R

R

I

R

A

I

S

I

M

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

I

V

L

L

T

T

-

D

P

P

A

A

Q

K

G

G

S

-

M

M

E

K

A

G

A

A

I

I

D

A

L

K

A

A

R

E

G

E

-

I

M

L

E

A

E

K

D

T

G

P

Q

N

G

G

R

Y

I

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

Q

G

G

G

T

K

I

I

T

D

H

G

F

F

V

V

S

S

A

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

A

A

S

N

V

V

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

E

V

G

E

A

S

S

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

R

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

D

N

A

V

Y

D

R

L

V

I

D

D

Q

E

I

V

E

Q

V

V

N

S

Q

S

T

D

D

E

A

S

E

I

D

D

T

M

T

A

R

R

A

Q

L

L

A

A

A

L

Q

K

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

F

A

A

A

L

I

Q

K

L

L

A

A

K

Q

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

F

V

V

A

A

I

I

I

F

C
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

active site: A44 (≠ K43), S267 (= S257)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G72), N75 (= N73), T76 (= T74), Q145 (= Q142), I178 (≠ M175), G179 (= G176), T180 (= T177), G181 (≠ T178), T183 (= T180), G223 (vs. gap), S267 (= S257), P294 (≠ C282), S295 (≠ D283)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
44% identity, 96% coverage: 10:294/296 of query aligns to 11:305/306 of 2q3dA

query
sites
2q3dA

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

D

G

T

A

S

V

N

A

T

D

I

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

K

Q

H

A

A

A

E

E

A

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

R

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

K

R

M

C

T

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

A

L

E

E

R

R

I

R

A

M

S

L

M

L

K

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

A

G

Q

A

G

D

S

G

M

M

E

S

A

G

A

A

I

I

D

A

L

K

A

A

R

E

G

E

M

L

E

A

E

K

D

T

G

D

Q

Q

G

R

R

Y

I

F

L

V

-

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

A

I

H

H

Y

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

Q

D

G

G

T

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

S

S

V

A

Q

R

I

F

V

V

G

A

V

V

Q

E

P

P

E

A

G

A

A

S

S

P

K

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

R

H

E

P

-

I

-

Q

-
x
G

-
x
I

-

G

-

A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

D

D

A

Q

Y

D

R

L

V

V

D

D

Q

E

I

I

E

T

V

V

N

G

Q

N

T

E

D

D

A

A

E

L

D

N

T

V

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

F

V

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

V

A

V

I

V

I

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K44 (= K43), S266 (= S257), P293 (≠ C282)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (= Q142), V177 (≠ M175), G178 (= G176), T179 (= T177), G180 (≠ T178), T182 (= T180), G222 (vs. gap), I223 (vs. gap), S266 (= S257), P293 (≠ C282), D294 (= D283)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 96% coverage: 10:294/296 of query aligns to 11:305/310 of P9WP55

query
sites
P9WP55

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

D

G

T

A

S

V

N

A

T

D

I

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

K

Q

H

A

A

A

E

E

A

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

R

I

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

K

R

M

C

T

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

A

L

E

E

R

R

I

R

A

M

S

L

M

L

K

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

A

G

Q

A

G

D

S

G

M

M

E

S

A

G

A

A

I

I

D

A

L

K

A

A

R

E

G

E

M

L

E

A

E

K

D

T

G

D

Q

Q

G

R

R

Y

I

F

L

V

-

P

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

A

I

H

H

Y

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

Q

D

G

G

T

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

S

S

V

A

Q

R

I

F

V

V

G

A

V

V

Q

E

P

P

E

A

G

A

A

S

S

P

K

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

R

H

E

P

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

D

D

A

Q

Y

D

R

L

V

V

D

D

Q

E

I

I

E

T

V

V

N

G

Q

N

T

E

D

D

A

A

E

L

D

N

T

V

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

F

V

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

V

A

V

I

V

I

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 176:180) binding pyridoxal 5'-phosphate
S266 (= S257) binding pyridoxal 5'-phosphate

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
42% identity, 100% coverage: 2:296/296 of query aligns to 10:314/330 of 8b9yC

query
sites
8b9yC

I

V

F

A

P

P

T

S

V

M

E

D

S

A

Q

L

V

I

G

G

N

E

T

T

P

P

L

A

V

V

R

Y

L

L

Q

K

R

R

L

M

P

-

G

N

D

D

T

T

S

A

N

A

T

T

I

I

L

V

A

L

K

K

L

L

E

E

G

C

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

S

A

M

I

I

Y

K

D

H

K

A

A

E

E

A

K

R

E

G

G

E

K

I

I

K

I

P

P

G

G

D

K

R

S

L

V

I

I

-

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

H

A

I

A

G

A

T

I

I

K

R

G

G

Y

Y

K

K

M

V

T

I

L

I

I

V

M

M

P

P

D

E

N

S

M

M

S

S

A

I

E

E

R

R

I

R

A

C

S

L

M

M

K

R

A

I

Y

F

G

G

A

A

E

E

I

V

I

I

L

L

T

T

P

P

A

A

Q

A

G

L

S

G

M

M

E

K

A

G

A

A

I

L

D

E

-

A

L

V

A

N

R

R

G

I

M

V

E

S

E

N

D

N

G

P

Q

D

G

A

R

V

I

S

L

A

D

N

Q

Q

F

F

A

A

N

T

P

K

D

Y

N

N

P

A

R

Q

A

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

Q

K

G

G

T

H

I

V

T

D

H

C

F

F

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

R

Y

Y

L

L

K

K

E

S

Q

V

N

G

A

C

S

G

V

A

Q

T

I

I

V

F

G

A

V

V

Q

E

P

P

E

A

G

E

A

S

S

P

K

V

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

R

H

E

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

E

I

V

Y

F

D

E

A

A

Y

A

R

L

V

V

D

D

Q

E

I

V

I

I

E

Q

V

V

N

S

Q

G

T

D

D

E

A

A

E

I

D

D

T

T

T

A

R

Q

A

K

L

L

A

P

A

R

Q

T

E

D

G

G

I

I

F

F

A

C

G

G

V

F

S
|
S

S

G

G

G

G

A

A

N

V

V

F

Y

A

A

L

L

Q

Q

L

I

A

A

K

K

Q

R

V

P

E

E

N

M

A

A

-

G

-

K

I

T

I

I

V

V

A

T

I

V

I

I

C
x
P

D
x
S

R

Y

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

Y

P

S

T

S

binding pyridoxal-5'-phosphate: K50 (= K43), N81 (= N73), G185 (= G176), T186 (= T177), G187 (≠ T178), T189 (= T180), G229 (vs. gap), S273 (= S257), P300 (≠ C282), S301 (≠ D283)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
44% identity, 98% coverage: 6:294/296 of query aligns to 7:306/309 of 7n2tA

query
sites
7n2tA

V

V

E

T

S

E

Q

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

R

L

V

P

V

G

D

D

G

T

C

S

V

N

A

T

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

I

K

S

H

D

A

A

E

E

A

N

R

K

G

G

E

L

I

I

K

T

P

P

G

G

D

E

R

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

A

K

K

G

G

Y

Y

K

R

M

L

T

I

L

I

I

C

M

M

P

P

D

A

N

S

M

M

S

S

A

L

E

E

R

R

I

R

A

T

S

I

M

L

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

I

V

L

L

T

T

-

D

P

P

A

A

Q

R

G

G

S

-

M

M

E

K

A

G

A

A

I

V

D

Q

L

K

A

A

R

E

G

E

M

I

E

K

-

A

E

K

D

T

G

P

Q

N

G

S

R

Y

I

I

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

G

T

S

Q

G

G

G

T

K

I

I

T

D

H

A

F

L

V

V

S

S

A

G

M

I

G

G

T

T

T

G

G

G

T

T

I

V

M

T

G

G

T

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

A

P

S

N

V

I

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

E

V

G

E

A

S

S

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

R

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

G

I

V

Y

L

D

D

A

V

Y

N

R

L

V

L

D

D

Q

E

I

V

I

I

E

Q

V

V

N

S

Q

S

T

E

D

E

A

S

E

I

D

E

T

T

T

A

R

K

A

L

L

L

A

A

A

L

Q

K

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S

S

G

G

G

A

A

A

V

A

F

A

A

A

L

I

Q
x
R

L

I

A

A

K

K

Q
x
R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

V

A

A

I

V

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

binding phosphate ion: R277 (≠ Q267), R281 (≠ Q271)

8b9yA Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
43% identity, 99% coverage: 4:296/296 of query aligns to 10:297/313 of 8b9yA

query
sites
8b9yA

P

P

T

S

V

M

E

D

S

A

Q

L

V

I

G

G

N

E

T

T

P

P

L

A

V

V

R

Y

L

L

Q

K

R

R

L

M

P

-

G

N

D

D

T

T

S

A

N

A

T

T

I

I

L

V

A

L

K

K

L

L

E

E

G
x
C

N
x
E

N
|
N

P
|
P

A

M

G
x
A

S

S

V
|
V

K
|
K

D

D

R

R

P

L

A

A

L

Y

S

A

M

I

I

Y

K

D

H

K

A

A

E

E

A

K

R

E

G

G

E

K

I

I

K

I

P

P

G

G

D

K

R

S

L

V

I

I

-

V

E

E

A

A

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

H

A

I

A

G

A

T

I

I

K

R

G

G

Y

Y

K

K

M

V

T

I

L

I

I

V

M

M

P

P

D

E

N

S

M

M

S

S

A

I

E

E

R

R

I

R

A

C

S

L

M

M

K

R

A

I

Y

F

G

G

A

A

E

E

I

V

I

I

L

L

T

T

P

P

A

A

Q

A

G

L

S

G

M

M

E

K

A

G

A

A

I

L

D

E

-

A

L

V

A

N

R

R

G

I

M

V

E

S

E

N

D

N

G

P

Q

D

G

A

R

V

I

S

L

A

D

N

Q

Q

F

F

A

A

N

T

P

K

D

Y

N

N

P

A

R

Q

A

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

Q

K

G

G

T

H

I

V

T

D

H

C

F

F

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

R

Y

Y

L

L

K

K

E

S

Q

V

N

G

A

C

S

G

V

A

Q

T

I

I

V

F

G

A

V

V

Q

E

P

P

E

A

G

E

A

S

S

P

K

V

I

L

P

-

G

-

I

-

R

-

W

-

P

-

R

-

E

S

Y

F

L

V

P

P

K

E

I

V

Y

F

D

E

A

A

Y

A

R

L

V

V

D

D

Q

E

I

V

I

I

E

Q

V

V

N

S

Q

G

T

D

D

E

A

A

E

I

D

D

T

T

T

A

R

Q

A

K

L

L

A

P

A

R

Q

T

E

D

G

G

I

I

F

F

A

C

G

G

V

F

S
|
S

S

G

G

G

G

A

A

N

V

V

F

Y

A

A

L

L

Q

Q

L

I

A

A

K

K

Q

R

V

P

E

E

N

M

A

A

-

G

-

K

I

T

I

I

V

V

A

T

I

V

I

I

C
x
P

D
x
S

R
x
Y

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

Y

P

S

T

S

binding o-acetylserine: T76 (= T70), G78 (= G72), N79 (= N73), T80 (= T74)
binding pyridoxal-5'-phosphate: V47 (= V42), K48 (= K43), N79 (= N73), G183 (= G176), T184 (= T177), G185 (≠ T178), T187 (= T180), S256 (= S257), P283 (≠ C282), S284 (≠ D283)
binding alpha-D-ribofuranose: C40 (≠ G35), E41 (≠ N36), N42 (= N37), P43 (= P38), A45 (≠ G40), Y285 (≠ R284)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
43% identity, 96% coverage: 10:294/296 of query aligns to 15:310/341 of Q93244

query
sites
Q93244

V

I

G

G

N

N

T

T

P

P

L

M

V

V

R

Y

L

I

Q

N

R

K

L

L

P

T

G

K

D

G

T

L

S

P

N

G

T

T

I

V

L

A

A

V

K

K

L

I

E

E

G

Y

N

M

N

N

P

P

A

A

G

G

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

A

S

A

M

M

I

L

K

A

H

A

A

A

E

E

A

K

R

D

G

G

E

T

I

V

K

I

P

P

G

G

-

V

D

T

R

T

L

L

I

I

E

E

A
x
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

M

F

A

V

A
|
A

A

A

I

A

K

K

G

G

Y

Y

K

R

M

C

T

I

L

V

I

T

M

M

P

P

D

A

N

S

M

M

S

S

A

G

E

E

R

R

I

R

A

T

S

L

M

L

K

K

A

A

Y

Y

G

G

A

S

E

E

I

V

L

L

T

T

-

D

P

P

A

A

Q

K

G

G

S

-

M

M

E

K

A

G

A

A

I

I

D

D

L

M

A

A

R

N

G

Q

M

L

E

K

E

E

D

N

G

I

Q

P

G

G

R
x
S

I

I

-

I

L

L

D

A

Q

Q

F

F

A

D

N

N

P

P

D

N

N

N

P

P

R

L

A

V

H

H

Y

Y

E

Q

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

Q

K

G

G

T

T

I

V

T

D

H

A

F

V

V

V

S

F

A
x
G

M

V

G
|
G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

Q

E

E

Q

Q

N

N

A

P

S

G

V

V

Q

R

I

V

V

F

G

A

V

V

Q

E

P

P

E

E

G

E

A

S

S

A

K

I

I

L

P

S

G

G

I

R

R

P

R

A

W

G

P

P

R

H

E

K

-

I

-

Q

-

G

-

I

-
x
G

-

A

-

G

Y

F

L

A

P

P

K

A

I

V

Y

L

D

D

A

T

Y

K

R

I

V

Y

D

E

Q

D

I

V

I

I

E

R

V

I

N

H

Q

S

T

D

D

E

A

A

E

I

D

V

T

M

T

A

R

Q

A
x
R

L

L

A

S

A

Y

Q

E

E

E

G

G

I

L

F

L

A

G

G

G

V

I

S

S

S
|
S

G

G

G

A

A

N

V

V

F

A

A

A

L

L

Q

Q

L

L

A

A

K

A

Q

R

V

P

E

E

N

M

A

A

-

G

-

K

I

L

I

I

V

V

A
x
T

I

C

I

L

C

P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

M

S

T

T

S

G

P

V

L

F

Y

P75 (≠ A69) mutation to L: In n5537; severe loss of protein stability.
A88 (= A82) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ R138) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (≠ A174) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G176) mutation to R: In n5515; severe loss of protein stability.
G229 (vs. gap) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (≠ A245) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S258) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ A279) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
44% identity, 95% coverage: 10:289/296 of query aligns to 11:300/300 of 3zeiA

query
sites
3zeiA

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

D

G

T

A

S

V

N

A

T

D

I

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

K

Q

H

A

A

A

E

E

A

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

R

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

K

R

M

C

T

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

A

L

E

E

R

R

I

R

A

M

S

L

M

L

K

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

A

G

Q

A

G

D

S

G

M

M

E

S

A

G

A

A

I
|
I

D

A

L

K

A

A

R

E

G

E

M

L

E

A

E

K

D

T

G

D

Q

Q

G

R

R

Y

I

F

L

V

-

P

D

Q

Q
|
Q

F
|
F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

A

I

H

H

Y

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

Q

D

G

G

T

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

S

S

V

A

Q

R

I

F

V

V

G

A

V

V

Q

E

P

P

E

A

G

A

A

S

S

P

K

V

I

L

P

S

G

G

I

G

R

Q

R
x
K

W

G

P

P

R

H

E

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

-

G

Y
x
F

L

V

P

P

K

P

I

V

Y

L

D

D

A

Q

Y

D

R

L

V

V

D

D

Q

E

I

I

E

T

V

V

N

G

Q

N

T

E

D

D

A

A

E

L

D

N

T

V

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

F

V

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

V

A

V

I

V

I

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S257), P293 (≠ C282)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T70), S72 (= S71), I126 (= I125), Q144 (= Q142), F145 (= F143), K215 (≠ R213), G222 (vs. gap), A225 (vs. gap), F227 (≠ Y218)
binding pyridoxal-5'-phosphate: K44 (= K43), N74 (= N73), V177 (≠ M175), G178 (= G176), T179 (= T177), G180 (≠ T178), T182 (= T180), G222 (vs. gap), S266 (= S257), P293 (≠ C282), D294 (= D283)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
44% identity, 95% coverage: 10:289/296 of query aligns to 11:300/300 of 2q3cA

query
sites
2q3cA

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

D

G

T

A

S

V

N

A

T

D

I

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

K

Q

H

A

A

A

E

E

A

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

R

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

K

R

M

C

T

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

A

L

E

E

R

R

I

R

A

M

S

L

M

L

K

R

A

A

Y

Y

G

G

A

A

E

E

I

L

I

I

L

L

T

T

P

P

A

G

Q

A

G

D

S

G

M
|
M

E

S

A

G

A

A

I

I

D

A

L

K

A

A

R

E

G

E

M

L

E

A

E

K

D

T

G

D

Q

Q

G

R

R

Y

I

F

L

V

-

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

A

I

H

H

Y

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

Q

D

G

G

T

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

T

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

A

P

S

S

V

A

Q

R

I

F

V

V

G

A

V

V

Q

E

P

P

E

A

G

A

A

S

S

P

K

V

I

L

P

S

G

G

I

G

R

Q

R
x
K

W

G

P

P

R

H

E

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

D

D

A

Q

Y

D

R

L

V

V

D

D

Q

E

I

I

E

T

V

V

N

G

Q

N

T

E

D

D

A

A

E

L

D

N

T

V

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

F

V

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

V

A

V

I

V

I

L

C
x
P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S257), P293 (≠ C282)
binding : T71 (= T70), S72 (= S71), G73 (= G72), T75 (= T74), M122 (= M121), Q144 (= Q142), K215 (≠ R213), G222 (vs. gap), A225 (vs. gap)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
44% identity, 98% coverage: 6:294/296 of query aligns to 3:301/302 of 2efyA

query
sites
2efyA

V

V

E

E

S

G

Q

A

V

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

R

K

L

V

P

V

G

E

D

P

T

D

S

M

N

A

T

E

I

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

S

Y

M

M

I

I

K

K

H

D

A

A

E

E

A

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

R

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

A

I

A

A

I

S

K

R

G

G

Y

Y

K

R

M

L

T

I

L

L

I

T

M

M

P

P

D

A

N

Q

M

M

S

S

A

E

E

E

R

R

I

K

A

R

S

V

M

L

K

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

V

L

L

T

T

P

D

A

P

Q

E

G

R

S

R

M

M

E

L

A

A

I

R

D

E

L

E

A

A

R

L

G

R

M

L

E

K

E

E

D

E

G

L

Q

G

G

A

R

F

I

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

R

R

A

A

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

Q

E

G

G

T

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

A

P

S

H

V

V

Q

K

I

V

V

I

G

A

V

V

Q
x
E

P

P

E

A

G

R

A
x
S

S

N

K

V

I

L

P

S

G

G

I

G

R

K

R

M

W

G

P

Q

R

H

-

G

-

F

-

Q

-
x
G

-
x
M

-

G

-
x
P

E

G

Y

F

L

I

P

P

K

E

I

N

Y

L

D

D

A

L

Y

S

R

L

V

L

D

D

Q

G

I

V

I

I

E

Q

V

V

N

W

Q

E

T

E

D

D

A

A

E

F

D

P

T

L

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

F

A

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

F

W

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

E

V

L

E

G

N

P

A

G

I

K

I

R

V

V

A

A

I

C

I

I

C

S

-
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (≠ Q201), S204 (≠ A205), S263 (= S257)
binding 5-oxohexanoic acid: T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q142), G175 (= G176), G219 (vs. gap), M220 (vs. gap), P222 (vs. gap)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S173), G175 (= G176), T176 (= T177), G177 (≠ T178), T179 (= T180), G219 (vs. gap), S263 (= S257), P289 (vs. gap), D290 (= D283)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
44% identity, 98% coverage: 6:294/296 of query aligns to 3:301/302 of 2ecqA

query
sites
2ecqA

V

V

E

E

S

G

Q

A

V

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

R

K

L

V

P

V

G

E

D

P

T

D

S

M

N

A

T

E

I

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

S

Y

M

M

I

I

K

K

H

D

A

A

E

E

A

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

R

V

L

I

I

V

E

E

A

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

A

I

A

A

I

S

K

R

G

G

Y

Y

K

R

M

L

T

I

L

L

I

T

M

M

P

P

D

A

N

Q

M

M

S

S

A

E

E

E

R

R

I

K

A

R

S

V

M

L

K

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

V

L

L

T

T

P

D

A

P

Q

E

G

R

S

R

M

M

E

L

A

A

I

R

D

E

L

E

A

A

R

L

G

R

M

L

E

K

E

E

D

E

G

L

Q

G

G

A

R

F

I

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

R

R

A

A

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

Q

E

G

G

T

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A
x
G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

A

P

S

H

V

V

Q

K

I

V

V

I

G

A

V

V

Q
x
E

P

P

E

A

G

R

A
x
S

S

N

K

V

I

L

P

S

G

G

I

G

R

K

R

M

W

G

P

Q

R

H

-

G

-

F

-

Q

-
x
G

-

M

-

G

-

P

E

G

Y

F

L

I

P

P

K

E

I

N

Y

L

D

D

A

L

Y

S

R

L

V

L

D

D

Q

G

I

V

I

I

E

Q

V

V

N

W

Q

E

T

E

D

D

A

A

E

F

D

P

T

L

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

F

A

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

F

W

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

E

V

L

E

G

N

P

A

G

I

K

I

R

V

V

A

A

I

C

I

I

C

S

-
x
P

D

D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (≠ Q201), S204 (≠ A205), S263 (= S257)
binding (3s)-3-hydroxybutanoic acid: K40 (= K43), G71 (= G72), T73 (= T74), Q141 (= Q142), G219 (vs. gap)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S173), G173 (≠ A174), G175 (= G176), T176 (= T177), T179 (= T180), G219 (vs. gap), S263 (= S257), P289 (vs. gap)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
44% identity, 98% coverage: 6:294/296 of query aligns to 3:301/302 of 2ecoA

query
sites
2ecoA

V

V

E

E

S

G

Q

A

V

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

R

K

L

V

P

V

G

E

D

P

T

D

S

M

N

A

T

E

I

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

S

Y

M

M

I

I

K

K

H

D

A

A

E

E

A

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

R

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

A

I

A

A

I

S

K

R

G

G

Y

Y

K

R

M

L

T

I

L

L

I

T

M

M

P

P

D

A

N

Q

M

M

S

S

A

E

E

E

R

R

I

K

A

R

S

V

M

L

K

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

V

L

L

T

T

P

D

A

P

Q

E

G

R

S

R

M

M

E

L

A

A

I

R

D

E

L

E

A

A

R

L

G

R

M

L

E

K

E

E

D

E

G

L

Q

G

G

A

R

F

I

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

R

R

A

A

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

Q

E

G

G

T

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

A

P

S

H

V

V

Q

K

I

V

V

I

G

A

V

V

Q
x
E

P

P

E

A

G

R

A
x
S

S

N

K

V

I

L

P

S

G

G

I

G

R

K

R

M

W

G

P

Q

R

H

-

G

-

F

-

Q

-
x
G

-

M

-

G

-

P

E

G

Y

F

L

I

P

P

K

E

I

N

Y

L

D

D

A

L

Y

S

R

L

V

L

D

D

Q

G

I

V

I

I

E

Q

V

V

N

W

Q

E

T

E

D

D

A

A

E

F

D

P

T

L

T

A

R

R

A

R

L

L

A

A

A

R

Q

E

E

E

G

G

I

L

F

F

A

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

F

W

A

A

L

L

Q

Q

L

V

A

A

K

R

Q

E

V

L

E

G

N

P

A

G

I

K

I

R

V

V

A

A

I

C

I

I

C

S

-
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (≠ Q201), S204 (≠ A205), S263 (= S257)
binding 4-methyl valeric acid: K40 (= K43), T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q142), G175 (= G176), T176 (= T177), G219 (vs. gap)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S173), G175 (= G176), T176 (= T177), T179 (= T180), G219 (vs. gap), S263 (= S257), P289 (vs. gap), D290 (= D283)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 96% coverage: 10:294/296 of query aligns to 83:378/392 of P47999

query
sites
P47999

V

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

N

R

N

L

V

P

V

G

K

D

G

T

C

S

V

N

A

T

S

I

V

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

I

K

T

H

D

A

A

E

E

A

E

R

K

G

G

E

L

I

I

K

T

P

P

G

G

D

K

R

S

-

V

L

L

I

V

E

E

A

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

S

K

K

G

G

Y

Y

K

K

M

L

T

I

L

L

I

T

M

M

P

P

D

A

N

S

M

M

S

S

A

L

E

E

R

R

I

R

A

V

S

L

M

L

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

I

V

L

L

T

T

-

E

P

P

A

A

Q

K

G

G

S

-

M

M

E

T

A

G

A

A

I

I

D

Q

L

K

A

A

R

E

G

E

-

I

M

L

E

K

D

T

G

P

Q

N

G

S

R

Y

I

M

L

L

D

Q

Q

Q

F

F

A

D

N

N

P

P

D

A

N

N

P

P

R

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

Q

R

G

G

T

K

I

I

T

D

H

I

F

L

V

V

S

A

A

G

M

I

G

G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

T

V

S

G

R

R

Y

F

L

I

K

K

E

E

Q

R

N

K

A

P

S

E

V

L

Q

K

I

V

V

I

G

G

V

V

Q

E

P

P

E

T

G

E

A

S

S

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

R

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

K

I

N

Y

L

D

D

A

L

Y

A

R

I

V

V

D

D

Q

E

I

Y

I

I

E

A

V

I

N

S

Q

S

T

E

D

E

A

A

E

I

D

E

T

T

T

S

R

K

A

Q

L

L

A

A

A

L

Q

Q

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S

S

G

G

G

A

A

A

V

A

F

A

A

A

L

I

Q

Q

L

V

A

A

K

K

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

A

A

V

I

V

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

Q

V

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
42% identity, 98% coverage: 6:294/296 of query aligns to 17:316/323 of 4aecA

query
sites
4aecA

V

V

E

S

S

Q

Q

L

V

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

N

R

S

L

I

P

A

G

K

D

G

T

C

S

V

N

A

T

N

I

I

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

V

K

T

H

D

A

A

E

E

A

Q

R

K

G

G

E

F

I

I

K

S

P

P

G

G

D

K

R

S

-

V

L

L

I

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

S

K

R

G

G

Y

Y

K

R

M

L

T

I

L

L

I

T

M

M

P

P

D

A

N

S

M

M

S

S

A

M

E

E

R

R

I

R

A

V

S

L

M

L

K

K

A

A

Y

F

G

G

A

A

E

E

I

L

I

V

L

L

T

T

-

D

P

P

A

A

Q

K

G

G

S

M

M

T

E

G

A

A

A

V

I

Q

D

K

L

A

A

E

R

E

G

I

M

L

E

K

E

N

D

T

G

P

Q

D

G

A

R

Y

I

M

L

L

D

Q

Q

Q

F

F

A

D

N

N

P

P

D

A

N

N

P

P

R

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

D

D

D

T

T

Q

K

G

G

T

K

I

V

T

D

H

I

F

F

V

V

S

A

A

G

M
x
I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

F

L

I

K

K

E

E

Q

K

N

N

A

P

S

K

V

T

Q

Q

I

V

V

I

G

G

V

V

Q

E

P

P

E

T

G

E

A

S

S

D

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

R

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

K

I

N

Y

L

D

D

A

Q

Y

K

R

I

V

M

D

D

Q

E

I

V

I

I

E

A

V

I

N

S

Q

S

T

E

D

E

A

A

E

I

D

E

T

T

T

A

R

K

A

Q

L

L

A

A

A

L

Q

K

E

E

G

G

I

L

F

M

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

F

A

A

A

L

I

Q

K

L

V

A

A

K

K

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

I

L

I

I

V

A

A

V

I

V

I

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K54 (= K43), S277 (= S257)
binding pyridoxal-5'-phosphate: K54 (= K43), N85 (= N73), I188 (≠ M175), G189 (= G176), T190 (= T177), G191 (≠ T178), G192 (= G179), T193 (= T180), G233 (vs. gap), S277 (= S257), P304 (≠ C282)

Query Sequence

>WP_013819023.1 NCBI__GCF_000214665.1:WP_013819023.1
MIFPTVESQVGNTPLVRLQRLPGDTSNTILAKLEGNNPAGSVKDRPALSMIKHAEARGEI
KPGDRLIEATSGNTGIALAMAAAIKGYKMTLIMPDNMSAERIASMKAYGAEIILTPAQGS
MEAAIDLARGMEEDGQGRILDQFANPDNPRAHYEGTGPEIWRDTQGTITHFVSAMGTTGT
IMGTSRYLKEQNASVQIVGVQPEGASKIPGIRRWPREYLPKIYDAYRVDQIIEVNQTDAE
DTTRALAAQEGIFAGVSSGGAVFAALQLAKQVENAIIVAIICDRGDRYLSTGVFPT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory