SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013819186.1 NCBI__GCF_000214665.1:WP_013819186.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
40% identity, 98% coverage: 5:611/619 of query aligns to 7:560/562 of 6ovtA

query
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6ovtA

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binding fe2/s2 (inorganic) cluster: C51 (≠ G49), G84 (= G82), C124 (= C122), D125 (= D123), A200 (≠ S200), C201 (= C201)
binding magnesium ion: D83 (= D81), D125 (= D123), K126 (= K124), E452 (= E496)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
40% identity, 98% coverage: 5:611/619 of query aligns to 20:573/575 of P9WKJ5

query
sites
P9WKJ5

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C64 (≠ G49) binding [2Fe-2S] cluster
D96 (= D81) binding Mg(2+)
C137 (= C122) binding [2Fe-2S] cluster
D138 (= D123) binding Mg(2+)
K139 (= K124) binding via carbamate group; modified: N6-carboxylysine
C214 (= C201) binding [2Fe-2S] cluster
E465 (= E496) binding Mg(2+)
S491 (= S522) mutation to A: Loss of catalytic activity.

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 98% coverage: 6:613/619 of query aligns to 57:607/608 of Q9LIR4

query
sites
Q9LIR4

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L

K

L

D

K

L

L

G

S

Q

E

L

A

V

V

A

K

R

E

E

G

I

V

E

E

Q

N

A

A

G

G

G

M

V

V

A

G

K

F

E

R

F

F

D

N

T

T

I

I

A

G

V

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

D

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

C

W

A

Y

D

D

A

G

L

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K

K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

M

G

R

R

I

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

H

V

-

R

-

L

-

A

-

N

-

P

-

D

-

S

-

Q

-

A

-

I

F

E

Q

F

D

K

K

K

T

L

Y

D

D

L

I

I

V

D

S

A

A

M

F

V

Q

M

S

A

Y

A

G

D

E

-

F

-

V

-

S

S

G

K

S

V

I

S

S

D

D

T

E

D

Q

L

R

L

K

E

T

V

V

E

L

R

H

S

H

A

S

C

C

P

P

T

G

C

A

G

G

S

A

C
|
C

S

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

T

I

E

E

A

A

L

M

G

G

L

M

S

S

L

L

P

P

G

Y

N

S

G

S

T

S

V

I

V

P

A

A

T

E

H

D

A

P

D

L

R

K

E

L

Q

D

L

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F

C

K

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Q

L

A

A

G

G

R

K

R

Y

I

L

V

L

D

E

L

L

A

L

K

K

Q

M

Y

D

Y

L

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K

Q

P

D

R

D

D

E

-

S

I

V

I

L

T

P

P

R

K

S

S

V

-

G

-

F

-

K

-

A

-

F

L

E

R

N

N

A

A

I

M

A

V

L

S

D

V

I

M

A

A

M

L

G

G

G

G

S

S

T

T

N

N

T

A

I

V

L

L

H

H

L

L

L

I

A

A

I

I

A

A

Q

R

E

S

A

V

G

G

I

L

D

E

F

L

T

T

M

L

A

D

D

D

I

F

D

Q

R

K

M

V

S

S

K

D

L

A

V

V

P

P

Q

F

L

L

C

A

K

D

V

L

A

K

P

P

N

-

T

S

N

G

K

K

Y

Y

H

V

I

M

E

E

D

D

V

I

H

H

R

K

A

I

G

G

G

G

I

T

M

P

A

A

I

V

L

L

A

R

E

Y

L

L

N

L

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A

L

G

G

R

L

L

M

H

D

T

G

D

D

V

C

P

M

T

T

V

V

H

T

A

G

K

Q

T

T

L

L

G

A

D

Q

A

N

L

L

A

E

Q

N

W

-

D

-

I

-

M

-

A

-

N

-

P

-

S

-

D

-

A

-

V

-

K

-

T

-

F

-

Y

-

M

-

A

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G

-

P

-

A

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G

-

I

-

P

-

S

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Q

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V

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A

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F

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S

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Q

-

N

-

T

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R

-

W

V

P

P

S

S

L

L

D

T

Q

E

D

-

R

-

A

G

E

Q

G

E

C

I

I

I

R

R

S

P

I

L

D

S

H

N

A

P

F

I

S

K

T

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E

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G

G

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L

I

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I

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L

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G

N

D

I

L

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A

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D

D

G

G

C

S

V

V

V

A

K

K

S

I

A

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G

G

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K

D

E

D

G

S

-

L

-

L

L

V

Y

F

F

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S

G

G

T

P

A

A

H

L

V

V

V

F

E

E

S

G

Q

E

D

E

E

S

A

M

V

L

E

A

N

A

I

I

L

S

A

A

D

D

K

P

V

M

K

S

-

F

A

K

G

G

D

T

V

V

V

V

V

V

V

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E
|
E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

I

I

K

M

S

G

K

A

G

G

L

L

G

G

K

K

A

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

V

G

G

H

H

C

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

G

G

L

L

V

I

R

K

N

N

G

G

D

D

R

I

I

I

R

T

I

I

D

D

I

I

P

G

N

K

R

K

T

R

I

I

N

D

V

T

L

Q

V

V

G

S

D

P

E

E

E

E

L

M

A

N

Q

D

R

R

R

R

A

K

E

K

Q

-

D

-

R

-

A

-

G

-

W

W

K

-

P

-

V

T

K

A

P

P

R

A

A

Y

R

K

K

V

V

N

S

R

V

G

A

V

L

L

R

Y

A

K

Y

Y

A

I

H

K

F

N

A

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

V

V

R

T

D

D

C100 (≠ G49) binding [2Fe-2S] cluster
C173 (= C122) binding [2Fe-2S] cluster
D174 (= D123) binding Mg(2+)
C245 (= C201) binding [2Fe-2S] cluster
E497 (= E496) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
36% identity, 98% coverage: 6:613/619 of query aligns to 19:569/570 of 8hs0A

query
sites
8hs0A

S

S

H

S

T

R

T

I

T

T

H

E

G

P

R

K

N

S

M

Q

A

G

G

G

A

S

R

Q

A

A

L

I

W

L

R

H

A

G

T

V

G

G

M

L

K

S

D

D

G

D

D

D

F

L

D

L

K

K

P

P

I

Q

I

I

A

G

I

I

A

S

N

S

S

V

F

W

T

Y

Q

E

F

G

V

N

P

T

G
x
C

H

N

V

M

H

H

L

L

K

L

D

K

L

L

G

S

Q

E

L

A

V

V

A

K

R

E

E

G

I

V

E

E

Q

N

A

A

G

G

G

M

V

V

A

G

K

F

E

R

F

F

D

N

T

T

I

I

A

G

V

V

D

S

D
|
D

G
x
A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

D

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

C

W

A

Y

D

D

A

G

L

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K
|
K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

M

G

R

R

I

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

H

V

-

R

-

L

-

A

-

N

-

P

-

D

-

S

-

Q

-

A

-

I

F

E

Q

F

D

K

K

K

T

L

Y

D

D

L

I

I

W

D

S

A

A

M

F

V

Q

M

S

A

Y

A

G

D

E

-

F

-

V

-

S

S

G

K

S

V

I

S

S

D

D

T

E

D

Q

L

R

L

K

E

T

V

V

E

L

R

H

S

H

A

S

C

C

P

P

T

G

C

A

G

G

S

A

C
|
C

S

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

T

I

E

E

A

A

L

M

G

G

L

M

S

S

L

L

P

P

G

Y

N

S

G

S

T

S

V

I

V

P

A

A

T

E

H

D

A

P

D

L

R

K

E

L

Q

D

L

E

F

C

K

R

Q

L

A

A

G

G

R

K

R

Y

I

L

V

L

D

E

L

L

A

L

K

K

Q

M

Y

D

Y

L

E

K

Q

P

D

R

D

D

E

-

S

I

V

I

L

T

P

P

R

K

S

S

V

-

G

-

F

-

K

-

A

-

F

L

E

R

N

N

A

A

I

M

A

V

L

S

D

V

I

M

A

A

M

L

G

G

G

G

S

S

T

T

N
|
N

T

A

I

V

L

L

H
|
H

L

L

L

I

A

A

I

I

A

A

Q

R

E

S

A

V

G

G

I

L

D

E

F

L

T

T

M

L

A

D

D

D

I

F

D

Q

R

K

M

V

S

S

K

D

L

A

V

V

P

P

Q

F

L

L

C

A

K

D

V

L

A

K

P

P

N

-

T

S

N

G

K

K

Y

Y

H

V

I

M

E

E

D

D

V

I

H

H

R

K

A

I

G

G

G

G

I

T

M

P

A

A

I

V

L

L

A

R

E

Y

L

L

N

L

R

E

A

L

G

G

R

L

L

M

H

D

T

G

D

D

V

C

P

M

T

T

V

V

H

T

A

G

K

Q

T

T

L

L

G

A

D

Q

A

N

L

L

A

-

Q

-

W

-

D

-

I

-

M

-

A

-

N

-

P

-

S

-

D

-

A

-

V

-

K

-

T

-

F

-

Y

-

M

-

A

-

G

-

P

-

A

-

G

-

I

-

P

-

S

-

Q

-

V

-

A

-

F

-

S

-

Q

E

N

N

T

V

R

-

W

-

P

P

S

S

L

L

D

T

Q

E

D

-

R

-

A

G

E

Q

G

E

C

I

I

I

R

R

S

P

I

L

D

S

H

N

A

P

F

I

S

K

T

E

E

T

G

G

G

H

L

I

A

Q

V

I

L

L

H

R

G

G

N

D

I

L

A

A

L

P

D

D

G

G

C

S

V

V

V

A

K

K

S

I

A

T

G

G

V

K

D

E

D

G

S

-

L

-

L

L

V

Y

F

F

E

S

G

G

T

P

A

A

H

L

V

V

V

F

E

E

S

G

Q

E

D

E

E

S

A

M

V

L

E

A

N

A

I

I

L

S

A

A

D

D

K

P

V

M

K

S

-

F

A

K

G

G

D

T

V

V

V

V

V

V

V

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E

E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

I

I

K

M

S

G

K

A

G

G

L

L

G

G

K

K

A

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

V

G

G

H

H

C

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

G

G

L

L

V

I

R

K

N

N

G

G

D

D

R

I

I

I

R

T

I

I

D

D

I

I

P

G

N

A

R

A

T

R

I

I

N

D

V

T

L

Q

V

V

G

S

D

P

E

E

E

E

L

M

A

N

Q

D

R

R

R

R

A

K

E

K

Q

-

D

-

R

-

A

-

G

-

W

W

K

-

P

-

V

T

K

A

P

P

R

A

A

Y

R

K

K

V

V

N

S

R

V

G

A

V

L

L

R

Y

A

K

Y

Y

A

I

H

K

F

N

A

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

V

V

R

T

D

D

binding fe2/s2 (inorganic) cluster: C62 (≠ G49), D94 (= D81), A95 (≠ G82), C135 (= C122), D136 (= D123), C207 (= C201)
binding magnesium ion: D136 (= D123), K137 (= K124), N285 (= N285), H289 (= H289)

8imuA Dihydroxyacid dehydratase (dhad) mutant-v497f
35% identity, 98% coverage: 6:613/619 of query aligns to 16:522/523 of 8imuA

query
sites
8imuA

S

S

H

S

T

R

T

I

T

T

H

E

G

P

R

K

N

S

M

Q

A

G

G

G

A

S

R

Q

A

A

L

I

W

L

R

H

A

G

T

V

G

G

M

L

K

S

D

D

G

D

D

D

F

L

D

L

K

K

P

P

I

Q

I

I

A

G

I

I

A

S

N

S

S

V

F

W

T

Y

Q

E

F

G

V
x
N

P

T

G
x
C

H

N

V

M

H

H

L

L

K

L

D

K

L

L

G

S

Q

E

L

A

V

V

A

K

R

E

E

G

I

V

E

E

Q

N

A

A

G

G

G

M

V

V

A

G

K

F

E

R

F

F

D

N

T

T

I

I

A

G

V

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

D

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

C

W

A

Y

D

D

A

G

L

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K

K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

M

G

R

R

I

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

-

V

-

R

-

L

-

A

-

N

-

P

-

D

-

S

-

Q

-

A

-

I

-

E

-

F

-

K

-

K

-

L

-

D

-

L

-

I

-

D

-

A

-

M

-

V

-

M

-

A

-

A

-

D

-

S

-

K

-

V

-

S

-

D

-

T

-

D

-

L

-

L

-

E

-

V

-

E

-

R

-

S

-

A

-

C

-

P

-

T

-

C

-

G

-

S

-

C

-

S

-

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

T

I

E

E

A

A

L

M

G

G

L

M

S

S

L

L

P

P

G

Y

N

S

G

S

T

S

V

I

V

P

A

A

T

E

H

D

A

P

D

L

R

K

E

L

Q

D

L

E

F

C

K

R

Q

L

A

A

G

G

R

K

R

Y

I

L

V

L

D

E

L

L

A

L

K

K

Q

M

Y

D

Y

L

E

K

Q

P

D

R

D

D

E

-

S

I

V

I

L

T

P

P

R

K

S

S

V

-

G

-

F

-

K

-

A

-

F

L

E

R

N

N

A

A

I

M

A

V

L

S

D

V

I

M

A

A

M

L

G

G

G

G

S

S

T

T

N

N

T

A

I

V

L

L

H

H

L

L

L

I

A

A

I

I

A

A

Q

R

E

S

A

V

G

G

I

L

D

E

F

L

T

T

M

L

A

D

D

D

I

F

D

Q

R

K

M

V

S

S

K

D

L

A

V

V

P

P

Q

F

L

L

C

A

K

D

V

L

A

K

P

P

N

-

T

S

N

G

K

K

Y

Y

H

V

I

M

E

E

D

D

V

I

H

H

R

K

A

I

G

G

G

G

I

T

M

P

A

A

I

V

L

L

A

R

E

Y

L

L

N

L

R

E

A

L

G

G

R

L

L

M

H

D

T

G

D

D

V

C

P

M

T

T

V

V

H

T

A

G

K

Q

T

T

L

L

G

A

D

Q

A

N

L

L

A

-

Q

-

W

-

D

-

I

-

M

-

A

-

N

-

P

-

S

-

D

-

A

-

V

-

K

-

T

-

F

-

Y

-

M

-

A

-

G

-

P

-

A

-

G

-

I

-

P

-

S

-

Q

-

V

-

A

-

F

-

S

-

Q

E

N

N

T

V

R

-

W

-

P

P

S

S

L

L

D

T

Q

E

D

-

R

-

A

G

E

Q

G

E

C

I

I

I

R

R

S

P

I

L

D

S

H

N

A

P

F

I

S

K

T

E

E

T

G

G

G

H

L

I

A

Q

V

I

L

L

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R

G

G

N

D

I

L

A

A

L

P

D

D

G

G

C

S

V

V

V

A

K

K

S

I

A

T

G

G

V

K

D

E

D

G

S

-

L

-

L

L

V

Y

F

F

E

S

G

G

T

P

A

A

H

L

V

V

V

F

E

E

S

G

Q

E

D

E

E

S

A

M

V

L

E

A

N

A

I

I

L

S

A

A

D

D

K

P

V

M

K

S

-

F

A

K

G

G

D

T

V

V

V

V

V

V

V

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E

E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

I

I

K

M

S

G

K

A

G

G

L

L

G

G

K

K

A

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

F

G

G

H

H

C

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

G

G

L

L

V

I

R

K

N

N

G

G

D

D

R

I

I

I

R

T

I

I

D

D

I

I

P

G

N

A

R

A

T

R

I

I

N

D

V

T

L

Q

V

V

G

S

D

P

E

E

E

E

L

M

A

N

Q

D

R

R

R

R

A

K

E

K

Q

-

D

-

R

-

A

-

G

-

W

W

K

-

P

-

V

T

K

A

P

P

R

A

A

Y

R

K

K

V

V

N

S

R

V

G

A

V

L

L

R

Y

A

K

Y

Y

A

I

H

K

F

N

A

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

V

V

R

T

D

D

binding fe2/s2 (inorganic) cluster: N57 (≠ V47), C59 (≠ G49), C132 (= C122), D133 (= D123)

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
32% identity, 97% coverage: 19:616/619 of query aligns to 20:557/569 of 8epzA

query
sites
8epzA

R

R

A

S

L

W

W

M

R

K

A

N

T

R

G

G

M

I

K

P

D

H

G

D

D

Q

F

F

D

D

-

G

K

R

P

P

I

V

I

I

A

G

I

I

A

C

N

N

S

T

F

F

T

S

Q

E

F

L

V

T

P

P

G
x
C

H

N

V

S

H

H

L

F

K

R

D

T

L

L

G

A

Q

E

L

Q

V

V

A

K

R

I

E

G

I

V

E

W

Q

E

A

S

G

G

G

G

V

F

A

P

K

L

E

E

F

F

D

P

T

V

I

M

A

S

V

L

D

G

D
x
E

G

T

I

M

A

-

M

L

G

R

H

P

D

T

G

A

M

M

L

L

Y

F

S

-

L

-

P

-

S

-

R

R

D

N

L

L

I

A

A

S

D

M

S

D

V

V

E

E

Y

E

M

S

V

I

N

R

A

G

H

N

C

P

A

L

D

D

A

G

L

V

V

V

C

L

I

L

S

M

N

G

C
|
C

D
|
D

K
|
K

I

T

T

T

P

P

G

S

M

L

L

M

M

M

A

G

A

A

M

A

R

S

I

C

N

D

I

L

P

P

V

T

I

I

F

G

V

V

S

S

G

G

G

G

P

P

M

M

E

L

A

S

G

G

K

K

V

F

R

R

L

G

A

R

N

E

P

L

D

G

S

S

Q

G

A

T

I

D

E

V

F

W

K

K

K

M

L

S

D

E

L

E

I

V

D

R

A

A

M

-

V

-

M

-

A

-

A

-

D

-

S

G

K

Q

V

M

S

S

D

Q

T

E

D

E

L

F

L

F

E

E

V

A

E

E

R

S

S

C

A

M

C

H

P

R

T

S

C

H

G

G

S

H

C
|
C

S

M

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

T

V

E

E

A

A

L

L

G

G

L

M

S

S

L

L

P

P

G

G

N

N

G

A

T

A

V

I

V

P

A

A

T

V

H

D

A

A

D

R

R

R

E

N

Q

L

L

L

F

A

K

R

Q

A

A

S

G

G

R

R

R

R

I

I

V

V

D

Q

L

M

A

V

K

K

Q

-

Y

-

Y

-

E

-

Q

-

D

-

D

D

E

D

S

L

V

V

L

M

P

S

R

K

S

I

V

L

G

T

F

R

K

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

L

V

D

N

I

A

A

A

M

I

G

G

G

G

S

S

T

T

N

N

T

A

I

V

L

I

H

H

L

L

L

L

A

A

I

I

A

A

Q

G

E

R

A

I

G

G

I

V

D

D

F

L

T

T

M

L

A

A

D

D

I

W

D

D

R

A

M

L

S

G

K

H

L

K

V

L

P

P

Q

C

L

L

C

V

K

D

V

L

A

Q

P

P

N

-

T

S

N

G

K

T

Y

H

H

L

I

M

E

E

D

D

V

F

H

Y

R

Y

A

A

G

G

G

G

I

V

M

P

A

A

I

V

L

I

A

R

E

E

L

L

N

G

R

D

A

V

G

-

R

-

L

I

H

A

T

R

D

D

V

A

P

L

T

T

V

V

H

N

A

G

K

Q

T

T

L

L

G

-

D

-

A

-

L

-

A

-

Q

-

W

W

D

D

I

-

M

-

A

-

N

N

P

C

S

K

D

D

A

A

V

-

K

-

T

-

F

-

Y

-

M

-

A

-

G

-

P

-

A

-

G

-

I

-

P

-

S

-

Q

-

V

-

A

-

F

-

S

-

Q

-

N

-

T

-

R

-

W

-

P

P

S

N

L

W

D

N

Q

R

D

E

R

-

A

-

E

-

G

-

C

V

I

I

R

H

S

A

I

F

D

N

H

E

A

P

F

F

S

K

T

T

E

E

G

A

G

G

L

I

A

A

V

V

L

L

H

R

G

G

N

N

I

L

A

C

L

P

D

D

G

G

C

A

V

V

V

I

K

K

S

P

A

S

G

A

V

A

D

T

D

P

S

A

L

L

L

L

-

K

-

H

-

K

-

G

-

R

-

A

-

V

V

V

F

F

E

E

G

N

T

S

A

E

H

H

V

M

V

H

E

E

S

R

Q

M

D

D

E

D

A

-

V

-

E

E

N

N

I

L

L

D

A

V

D

D

K

E

V

-

K

-

A

-

G

N

D

C

V

V

V

L

V

V

V

L

R

K

Y

N

E

C

G

G

P

P

K

R

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

-

A

-

G

-

N

M

M

L

P

Y

L

P

P

T

P

S

K

Y

I

I

L

K

R

S

-

K

K

G

G

L

I

G

T

K

D

A

M

C

V

A

R

L

-

L

V

T

S

D

D

G

A

R

R

F

M

S

S

G

G

G

T

T

A

S

Y

G

G

L

T

S

V

I

V

G

L

H

H

C

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

A

P

I

L

G

A

L

L

V

V

R

Q

N

D

G

G

D

D

R

I

I

I

R

E

I

L

D

D

I

V

P

A

N

A

R

R

T

K

I

L

N

H

V

L

L

H

V

V

G

S

D

D

E

E

E

E

L

L

A

A

Q

R

R

R

R

-

A

-

E

-

Q

-

D

-

R

R

A

E

G

A

W

W

K

Q

-

A

P

P

V

P

K

A

P

P

R

M

A

A

R

R

K

G

V

-

S

-

V

-

A

W

L

V

R

K

A

L

Y

Y

A

V

H

E

F

H

A

V

T

Q

S

Q

A

A

D

N

K

L

G

G

A

A

V

D

R

L

D

D

L

F

S

L

K

R

binding fe2/s2 (inorganic) cluster: C51 (≠ G49), C119 (= C122), D120 (= D123), C192 (= C201)
binding manganese (ii) ion: E83 (≠ D81), D120 (= D123), K121 (= K124), E443 (= E496)

8ej0A Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mg
32% identity, 97% coverage: 19:616/619 of query aligns to 19:556/568 of 8ej0A

query
sites
8ej0A

R

R

A

S

L

W

W

M

R

K

A

N

T

R

G

G

M

I

K

P

D

H

G

D

D

Q

F

F

D

D

-

G

K

R

P

P

I

V

I

I

A

G

I

I

A

C

N

N

S

T

F

F

T

S

Q

E

F

L

V

T

P

P

G
x
C

H

N

V

S

H

H

L

F

K

R

D

T

L

L

G

A

Q

E

L

Q

V

V

A

K

R

I

E

G

I

V

E

W

Q

E

A

S

G

G

G

G

V

F

A

P

K

L

E

E

F

F

D

P

T

V

I

M

A

S

V

L

D

G

D
x
E

G

T

I

M

A

-

M

L

G

R

H

P

D

T

G

A

M

M

L

L

Y

F

S

-

L

-

P

-

S

-

R

R

D

N

L

L

I

A

A

S

D

M

S

D

V

V

E

E

Y

E

M

S

V

I

N

R

A

G

H

N

C

P

A

L

D

D

A

G

L

V

V

V

C

L

I

L

S

M

N

G

C
|
C

D
|
D

K
|
K

I

T

T

T

P

P

G

S

M

L

L

M

M

M

A

G

A

A

M

A

R

S

I

C

N

D

I

L

P

P

V

T

I

I

F

G

V

V

S

S

G

G

G

G

P

P

M

M

E

L

A

S

G

G

K

K

V

F

R

R

L

G

A

R

N

E

P

L

D

G

S

S

Q

G

A

T

I

D

E

V

F
x
W

K

K

K

M

L

S

D

E

L

E

I

V

D

R

A

A

M

-

V

-

M

-

A

-

A

-

D

-

S

G

K

Q

V

M

S

S

D

Q

T

E

D

E

L

F

L

F

E

E

V

A

E

E

R

S

S

C

A

M

C

H

P

R

T

S

C

H

G

G

S

H

C
|
C

S

M

G

T

M

M

F

G

T
|
T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

T

V

E

E

A

A

L

L

G

G

L

M

S

S

L

L

P

P

G

G

N

N

G

A

T

A

V

I

V

P

A

A

T

V

H

D

A

A

D

R

R

R

E

N

Q

L

L

L

F

A

K

R

Q

A

A

S

G

G

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binding bicarbonate ion: D119 (= D123), W161 (≠ F165), T196 (= T206), E442 (= E496), S469 (= S522), S469 (= S522), G470 (= G523)
binding fe2/s2 (inorganic) cluster: C50 (≠ G49), E82 (≠ D81), C118 (= C122), D119 (= D123), C191 (= C201)
binding magnesium ion: E82 (≠ D81), D119 (= D123), K120 (= K124), E442 (= E496)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
33% identity, 92% coverage: 13:584/619 of query aligns to 22:537/579 of B5ZZ34

query
sites
B5ZZ34

R

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C59 (≠ G49) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (≠ D81) binding Mg(2+)
C127 (= C122) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D123) binding Mg(2+)
C200 (= C201) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (vs. gap) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ E486) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E496) binding Mg(2+)
S480 (= S522) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
32% identity, 92% coverage: 13:584/619 of query aligns to 19:534/576 of 5j85A

query
sites
5j85A

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T

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|
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D

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W

D

D

R

R

M

C

S

G

K

R

L

D

V

V

P

P

Q

T

L

I

C

V

K

N

V

L

A

M

P

P

N

-

T

S

N

G

K

K

Y

Y

H

L

I

M

E

E

D

E

V

F

H

F

R

Y

A

A

G

G

G

G

I

L

M

P

A

V

I

V

L

L

A

K

E

R

L

L

N

G

R

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A

A

G

G

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L

L

L

H

H

T

K

D

D

V

A

P

L

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V

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S

A

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K

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T

T

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G

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D

D

A

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L

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A

-

Q

-

W

-

D

-

I

-

M

-

A

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N

-

P

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K

D

D

A

V

V

V

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N

T

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F

-

Y

-

M

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P

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A

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G

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I

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W

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G

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N

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Q

I

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D

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Y

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K

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A

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K

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I

L

N

A

D

D

D

K

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L

K

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A

I

G

D

D

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-

N

-

C

V

I

V

M

V

V

V

M

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K

Y

N

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C

G

G

P

P

K

K

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

-

V

-

G

-

N

M

M

L

G

Y

L

P

P

T

P

S

K

Y

V

I

L

K

K

S

-

K

K

G

G

L

I

G

L

K

D

A

M

C

V

A

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L

-

L

I

T

S

D

D

G

A

R

R

F

M

S

A

G

G

G

T

T

A

S

Y

G

G

L

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I

V

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H

H

C

T

S

S

P

P

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E

A

A

A

A

A

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G

G

G

G

A

P

I

L

G

A

L

V

V

V

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K

N

N

G

G

D

D

R

M

I

I

R

E

I

L

D

D

I

V

P

P

N

N

R

R

T

R

I

L

N

H

V

L

L

D

V

I

G

S

D

D

E

E

E

E

L

L

A

A

Q

R

R

R

R

L

A

A

E

E

Q

-

D

-

R

-

A

-

G

-

W

W

K

Q

P

P

binding fe2/s2 (inorganic) cluster: C56 (≠ G49), N89 (≠ G82), C124 (= C122), D125 (= D123), T196 (≠ S200), C197 (= C201)
binding magnesium ion: E88 (≠ D81), D125 (= D123), K126 (= K124), T202 (= T206), E450 (= E496)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
31% identity, 98% coverage: 10:614/619 of query aligns to 16:569/583 of Q1JUQ1

query
sites
Q1JUQ1

T

T

H

N

G

D

R

K

N

N

M

G

A

F

G

M

A

Y

R

R

A

S

L

W

W

M

R

K

A

N

T

Q

G

G

M

I

K

P

D

D

G

H

D

E

F

F

D

D

-

G

K

R

P

P

I

I

I

I

A

G

I

I

A
x
C

N

N

S

T

F

W

T

S

Q

E

F

L

V

T

P

P

G
x
C

H

N

V

A

H

H

L

F

K

R

D

K

L

L

G

A

Q

E

L

H

V

V

A

K

R

R

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G

I

I

E

S

Q

E

A

A

G

G

G

G

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F

A

P

K

V

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E

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F

D

P

T

V

I

F

A

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N

D

G

D

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G

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I

-

A

N

M

L

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A

M

M

L

L

Y

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S

-

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T

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R

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N

L

L

I

A

A

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D

M

S

D

V

V

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A

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I

N

R

A

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P

A

I

D

D

A

A

L

V

V

V

C

L

I

L

S

A

N

G

C
|
C

D

D

K

K

I

T

T

T

P

P

G

A

M

L

L

L

M

M

A

G

A

A

M

A

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S

I

C

N

D

I

V

P

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V

A

I

I

F

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V

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S

G

G

G

G

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P

M

M

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L

A

N

G

G

K

K

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L

R

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L

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A

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N

N

P

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S

S

Q

G

A

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F

W

K

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K

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G

A

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C

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A

G

G

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|
C

S

N

G

T

M

M

F

G

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T

A

A

N

S

S

T

M

M

N

A

C

C

L

M

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A

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E

A

A

L

L

G

G

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S

A

L

L

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P

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N

N

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A

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A

A

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F

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N

N

A

A

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N

I

A

A

A

M

I

G

G

G

G

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N

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A

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W

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G

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N

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N

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D

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I

K

D

A

A

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S

D

S

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V

V

M

V

V

V

L

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Y

N

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x
C

G

G

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P

K

R

G

G

G

Y

P

P

G

G

M

M

Q

A

E

E

-

V

-

G

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N

M

M

L

G

Y

L

P

P

T

P

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K

Y

L

I

L

K

R

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-

K

Q

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G

L

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G

-

K

K

A

D

C

M

A

V

L

R

L

I

T

S

D

D

G

A

R

R

F

M

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S

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G

G

T

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A

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Y

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G

L

T

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I

V

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L

H

H

C

V

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A

P

P

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E

A

A

A

A

A

A

G

G

G

G

A

P

I

L

G

A

L

A

V

V

R

R

N

N

G

G

D

D

R

W

I

I

R

E

I

L

D

D

I

C

P

E

N

A

R

G

T

T

I

L

N

H

V

L

L

D

V

I

G

T

D

D

E

D

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E

L

L

A

H

Q

R

R

R

R

L

A

S

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D

Q

V

D

D

R

P

A

T

G

A

W

A

K

P

P

G

V

V

K

A

P

G

R

Q

A

L

R

G

K

K

V

G

S

G

V

Y

A

A

L

-

R

R

A

L

Y

Y

A

I

H

D

F

H

A

V

T

L

S

Q

A

A

D

D

K

E

G

G

A

C

V

D

R

L

D

D

L

F

C47 (≠ A40) mutation to A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
C56 (≠ G49) mutation to A: Loss of activity but seems to be able to bind a Fe-S cluster.
C124 (= C122) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C201) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (≠ E486) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

7m3kA Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
29% identity, 89% coverage: 34:581/619 of query aligns to 38:543/587 of 7m3kA

query
sites
7m3kA

K

R

P

P

I

I

I

I

A

G

I

I

A

A

N

Q

S

T

F

G

T

S

Q

D

F

L

V

S

P

P

G
x
C

H

N

V

R

H

H

L

H

K

L

D

E

L

L

G

A

Q

K

L

R

V

V

A

R

R

D

E

G

I

V

E

R

Q

E

A

A

G

G

G

G

V

I

A

V

K

I

E

E

F

F

D

P

T

V

I

H

A

P

V

I

D

Q

D

E

G

T

I

G

A

K

M

R

G

P

H

T

D

A

G

G

M

L

L

-

Y

-

S

-

L

-

P

-

S

D

R

R

D

N

L

L

I

A

A

Y

D

L

S

G

V

L

E

V

Y

E

M

V

V

L

N

Y

A

G

H

Y

C

P

A

L

D

D

A

G

L

V

V

V

C

L

I

T

S

I

N

G

C
|
C

D

D

K

K

I

T

T

T

P

P

G

A

M

C

L

L

M

M

A

A

A

A

M

A

R

T

I

V

N

N

I

I

P

P

V

A

I

I

F

A

V

L

S

S

G

V

G

G

P

P

M

M

E

L

A

N

G

G

K

W

V

F

R

R

L

G

A

E

N

R

P

T

D

G

S

S

Q

G

A

T

I

I

E

V

F

W

K

K

K

A

L

R

D

E

L

L

I

L

D

-

A

-

M

-

V

-

M

-

A

-

A

A

D

K

S

G

K

E

V

I

S

D

D

Y

T

Q

D

G

L

F

L

V

E

K

V

L

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V

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A

S

S

A

S

C

A

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P

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C

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G

G

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Y

C

C

S

N

G

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M

M

F

G

T

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A

A

N

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M

N

N

C

S

L

L

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A

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E

A

A

L

L

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G

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A

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Q

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A

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G

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V

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L

M

A

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K

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Q

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Y

D

Y

L

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K

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D

S

D

D

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-

S

-

V

I

L

L

P

T

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K

S

-

V

-

G

-

F

-

K

E

A

A

F

F

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I

N

N

A

A

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I

A

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L

V

D

N

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A

A

M

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G

G

G

G

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S

T

T

N

N

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L

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L

F

A

R

V

V

L

L

H

R

G

G

N

N

I

L

A

-

L

F

D

S

G

S

C

A

V

I

V

M

K

K

S

L

A

S

G

V

V

I

D

S

D

D

S

E

L

F

L

R

-

N

-

R

-

Y

-

L

-

S

-

D

-

A

-

K

-

D

-

P

-

N

V

A

F

F

E

E

G

G

T

K

A

A

H

V

V

V

V

F

E

D

S

G

Q

P

D

E

E

D

A

Y

V

H

E

H

N

R

I

I

-

D

-

D

L

P

A

A

D

L

K

E

V

I

K

D

A

E

G

H

D

T

V

V

V

L

V

F

V

M

R

R

Y

G

E

A

G

G

P

P

K

I

G

G

G

Y

P

P

G

G

M

A

Q

A

E

E

M

V

-

V

-

N

L

M

Y

R

P

A

T

P

S

D

Y

Y

I

L

K

L

S

K

K

K

G

G

L

I

G

-

K

T

A

S

C

L

A

P

L

C

L

I

T

G

D

D

G

G

R

R

F

Q

S

S

G

G

G

T

T

S

S

G

G

S

L

P

S

S

I

I

G

L

H

N

C

A

S

S

P

P

E

E

A

A

A

A

A

A

G

G

G

G

A

G

I

L

G

A

L

I

V

L

R

K

N

T

G

G

D

D

R

R

I

V

R

R

I

I

D

D

I

L

P

G

N

R

R

G

T

T

I

A

N

D

V

I

L

L

V

I

G

S

D

D

E

E

E

E

L

L

A

A

Q

E

R

R

R

R

A

K

E

A

Q

L

D

E

R

A

A

V

G

G

binding zinc ion: C53 (≠ G49), C121 (= C122)

5oynA Crystal structure of d-xylonate dehydratase in holo-form (see paper)
32% identity, 88% coverage: 34:576/619 of query aligns to 43:542/589 of 5oynA

query
sites
5oynA

K

K

P

P

I

I

I

I

A

G

I

I

A

A

N

Q

S

T

F

G

T

S

Q

D

F

I

V

S

P

P

-
x
C

G

N

H

R

V

I

H

H

L

L

K

-

D

D

L

L

G

V

Q

Q

L

R

V

V

A

R

R

D

E

G

I

I

E

R

Q

D

A

A

G

G

G

G

V

I

A

P

K

M

E

E

F

F

D

P

T

V

I

H

A

P

V

I

D

F

D
x
E

G
x
N

I

C

A

R

M

R

G

P

H

T

D

A

G

A

M

L

L

-

Y

-

S

-

L

-

P

-

S

D

R

R

D

N

L

L

I

S

A

Y

D

L

S

G

V

L

E

V

Y

E

M

T

V

L

N

H

A

G

H

Y

C

P

A

I

D

D

A

A

L

V

V

V

C

L

I

T

S

T

N

G

C
|
C

D
|
D

K
|
K

I

T

T

T

P

P

G

A

M

G

L

I

M

M

A

A

A

A

M

T

R

T

I

V

N

N

I

I

P

P

V

A

I

I

F

V

V

L

S

S

G

G

G

G

P

P

M

M

E

L

A

D

G

G

K

W

V

H

R

E

L

N

A

E

N

L

P

V

D

G

S

S

Q

G

A

T

I

V

E

I

F

W

K

R

K

S

L

R

D

R

L

K

I

L

D

A

A

A

M

-

V

-

M

-

A

-

A

-

D

-

S

G

K

E

V

I

S

T

D

E

T

E

D

E

L

F

L

I

E

D

V

R

E

A

R

A

S

S

A

S

C

A

P

P

T

S

C

A

G

G

S

H

C
|
C

S

N

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

N

C

A

L

V

T

A

E

E

A

A

L

L

G

G

L

L

S

S

L

L

P

T

G

G

N

C

G

A

T

A

V

I

V

P

A

A

T

P

H

Y

A

R

D

E

R

R

E

G

Q

Q

L

M

F

A

K

Y

Q

K

A

T

G

G

R

Q

R

R

I

I

V

V

D

D

L

L

A

A

K

-

Q

-

Y

Y

Y

D

E

D

Q

V

D

K

D

P

E

L

S

D

V

I

L

L

P

T

R

K

S

-

V

-

G

-

F

-

K

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

A

L

L

D

V

I

A

A

A

M

A

G

G

G

G

S

S

T

T

N

N

T

A

I

Q

L

P

H

H

L

I

L

V

A

A

I

M

A

A

Q

R

E

H

A

A

G

G

I

V

D

E

F

I

T

T

M

-

A

A

D

D

I

D

D

W

R

R

M

A

S

A

K

Y

L

D

V

I

P

P

Q

L

L

I

C

V

K

N

V

M

A

Q

P

P

N

-

T

A

N

G

K

K

Y

Y

H

L

I

G

E

E

D

R

V

F

H

H

R

R

A

A

G

G

G

G

I

A

M

P

A

A

I

V

L

L

A

W

E

E

L

L

N

L

R

Q

A

Q

G

G

R

R

L

L

H

H

T

G

D

D

V

V

P

L

T

T

V

V

H

T

A

G

K

K

T

T

L

M

G

S

D

E

A

N

L

L

A

Q

Q

G

W

R

D

E

I

T

M

-

A

-

N

-

P

-

S

S

D

D

A

R

V

E

K

V

T

I

F

F

Y

P

M

Y

A

H

G

E

P

P

A

-

G

-

I

-

P

-

S

-

Q

-

V

-

A

-

F

-

S

-

Q

-

N

-

T

-

R

-

W

-

P

-

S

-

L

-

D

-

Q

-

D

-

R

-

A

-

E

-

G

-

C

-

I

-

R

-

S

-

I

-

D

-

H

-

A

-

F

L

S

A

T

E

E

K

G

A

G

G

L

F

A

L

V

V

L

L

H

K

G

G

N

N

I

L

A

-

L

F

D

D

G

F

C

A

V

I

V

M

K

K

S

S

A

S

G

V

V

I

D

G

D

E

S

E

L

F

L

R

-

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

V

V

F

F

E

E

G

A

T

R

A

A

H

I

V

V

V

F

E

D

S

G

Q

S

D

D

E

D

A

Y

V

H

E

K

N

R

I

I

-

N

-

D

L

P

A

A

D

L

K

E

V

I

K

D

A

E

G

R

D

C

V

I

V

L

V

V

V

I

R

R

Y

G

E

A

G

G

P

P

K

I

G

G

G

W

P

P

G

G

M

S

Q

A

E
|
E

M

V

-

V

-

N

L

M

Y

Q

P

P

T

P

S

D

Y

H

I

L

K

L

S

K

K

K

G

G

L

I

G

-

K

M

A

S

C

L

A

P

L

T

L

L

T

G

D

D

G

G

R

R

F

Q

S

S

G

G

G

T

T

A

S

D

G

S

L

P

S

S

I

I

G

L

H

N

C

A

S

S

P

P

E

E

A

S

A

A

A

I

G

G

G

G

A

G

I

L

G

S

L

W

V

L

R

R

N

T

G

G

D

D

R

T

I

I

R

R

I

I

D

D

I

L

P

N

N

T

R

G

T

R

I

C

N

D

V

A

L

L

V

V

G

D

D

E

E

A

E

T

L

I

A

A

Q

A

R

R

R

K

A

Q

E

D

binding fe2/s2 (inorganic) cluster: C58 (vs. gap), N91 (≠ G82), C126 (= C122), D127 (= D123), C199 (= C201)
binding magnesium ion: E90 (≠ D81), D127 (= D123), K128 (= K124), E461 (= E496)

Q9A9Z2 D-xylonate dehydratase; XyDHT; Gluconate dehydratase; EC 4.2.1.82; EC 4.2.1.39 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see 2 papers)
32% identity, 88% coverage: 34:576/619 of query aligns to 49:548/595 of Q9A9Z2

query
sites
Q9A9Z2

K

K

P

P

I

I

I

I

A

G

I

I

A

A

N

Q

S

T

F

G

T

S

Q

D

F

I

V

S

P

P

-
x
C

G

N

H

R

V

I

H

H

L

L

K

-

D

D

L

L

G

V

Q

Q

L

R

V

V

A

R

R

D

E

G

I

I

E

R

Q

D

A

A

G

G

G

G

V

I

A

P

K

M

E

E

F

F

D

P

T

V

I

H

A

P

V

I

D

F

D
x
E

G

N

I

C

A

R

M

R

G

P

H

T

D

A

G

A

M

L

L

-

Y

-

S

-

L

-

P

-

S

D

R

R

D

N

L

L

I

S

A

Y

D

L

S

G

V

L

E

V

Y

E

M

T

V

L

N

H

A

G

H

Y

C

P

A

I

D

D

A

A

L

V

V

V

C

L

I

T

S

T

N

G

C
|
C

D
|
D

K

K

I

T

T

T

P

P

G

A

M

G

L

I

M

M

A

A

A

A

M

T

R

T

I

V

N

N

I

I

P

P

V

A

I

I

F

V

V

L

S

S

G

G

G

G

P

P

M

M

E

L

A

D

G

G

K

W

V

H

R

E

L

N

A

E

N

L

P

V

D

G

S

S

Q

G

A

T

I

V

E

I

F

W

K

R

K

S

L

R

D

R

L

K

I

L

D

A

A

A

M

-

V

-

M

-

A

-

A

-

D

-

S

G

K

E

V

I

S

T

D

E

T

E

D

E

L

F

L

I

E

D

V

R

E

A

R

A

S

S

A

S

C

A

P

P

T

S

C

A

G

G

S

H

C
|
C

S

N

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

N

C

A

L

V

T

A

E

E

A

A

L

L

G

G

L

L

S

S

L

L

P

T

G

G

N

C

G

A

T

A

V

I

V

P

A

A

T

P

H

Y

A

R

D

E

R

R

E

G

Q

Q

L

M

F

A

K

Y

Q

K

A

T

G

G

R

Q

R

R

I

I

V

V

D

D

L

L

A

A

K

-

Q

-

Y

Y

Y

D

E

D

Q

V

D

K

D

P

E

L

S

D

V

I

L

L

P

T

R

K

S

-

V

-

G

-

F

-

K

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

A

L

L

D

V

I

A

A

A

M

A

G

G

G

G

S

S

T

T

N

N

T

A

I

Q

L

P

H

H

L

I

L

V

A

A

I

M

A

A

Q

R

E

H

A

A

G

G

I

V

D

E

F

I

T

T

M

-

A

A

D

D

I

D

D

W

R

R

M

A

S

A

K

Y

L

D

V

I

P

P

Q

L

L

I

C

V

K

N

V

M

A

Q

P

P

N

-

T

A

N

G

K

K

Y

Y

H

L

I

G

E

E

D

R

V

F

H

H

R

R

A

A

G

G

G

G

I

A

M

P

A

A

I

V

L

L

A

W

E

E

L

L

N

L

R

Q

A

Q

G

G

R

R

L

L

H

H

T

G

D

D

V

V

P

L

T

T

V

V

H

T

A

G

K

K

T

T

L

M

G

S

D

E

A

N

L

L

A

Q

Q

G

W

R

D

E

I

T

M

-

A

-

N

-

P

-

S

S

D

D

A

R

V

E

K

V

T

I

F

F

Y

P

M

Y

A

H

G

E

P

P

A

-

G

-

I

-

P

-

S

-

Q

-

V

-

A

-

F

-

S

-

Q

-

N

-

T

-

R

-

W

-

P

-

S

-

L

-

D

-

Q

-

D

-

R

-

A

-

E

-

G

-

C

-

I

-

R

-

S

-

I

-

D

-

H

-

A

-

F

L

S

A

T

E

E

K

G

A

G

G

L

F

A

L

V

V

L

L

H

K

G

G

N

N

I

L

A

-

L

F

D

D

G

F

C

A

V

I

V

M

K

K

S

S

A

S

G

V

V

I

D

G

D

E

S

E

L

F

L

R

-

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

V

V

F

F

E

E

G

A

T

R

A

A

H

I

V

V

V

F

E

D

S

G

Q

S

D

D

E

D

A

Y

V

H

E

K

N

R

I

I

-

N

-

D

L

P

A

A

D

L

K

E

V

I

K

D

A

E

G

R

D
x
C

V

I

V

L

V

V

V

I

R

R

Y

G

E

A

G

G

P

P

K

I

G

G

G

W

P

P

G

G

M

S

Q

A

E
|
E

M

V

-

V

-

N

L

M

Y

Q

P

P

T

P

S

D

Y

H

I

L

K

L

S

K

K

K

G

G

L

I

G

-

K

M

A

S

C

L

A

P

L

T

L

L

T

G

D

D

G

G

R

R

F

Q

S

S

G

G

G

T

T

A

S

D

G

S

L

P

S

S

I

I

G

L

H

N

C

A

S

S

P

P

E

E

A

S

A

A

A

I

G

G

G

G

A

G

I

L

G

S

L

W

V

L

R

R

N

T

G

G

D

D

R

T

I

I

R

R

I

I

D

D

I

L

P

N

N

T

R

G

T

R

I

C

N

D

V

A

L

L

V

V

G

D

D

E

E

A

E

T

L

I

A

A

Q

A

R

R

R

K

A

Q

E

D

C64 (vs. gap) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
E96 (≠ D81) binding Mg(2+)
C132 (= C122) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
D133 (= D123) binding Mg(2+)
C205 (= C201) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
C450 (≠ D479) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E467 (= E496) binding Mg(2+)

Query Sequence

>WP_013819186.1 NCBI__GCF_000214665.1:WP_013819186.1
MPAYRSHTTTHGRNMAGARALWRATGMKDGDFDKPIIAIANSFTQFVPGHVHLKDLGQLV
AREIEQAGGVAKEFDTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADALVCIS
NCDKITPGMLMAAMRINIPVIFVSGGPMEAGKVRLANPDSQAIEFKKLDLIDAMVMAADS
KVSDTDLLEVERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGTVVATHADREQLFKQ
AGRRIVDLAKQYYEQDDESVLPRSVGFKAFENAIALDIAMGGSTNTILHLLAIAQEAGID
FTMADIDRMSKLVPQLCKVAPNTNKYHIEDVHRAGGIMAILAELNRAGRLHTDVPTVHAK
TLGDALAQWDIMANPSDAVKTFYMAGPAGIPSQVAFSQNTRWPSLDQDRAEGCIRSIDHA
FSTEGGLAVLHGNIALDGCVVKSAGVDDSLLVFEGTAHVVESQDEAVENILADKVKAGDV
VVVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALLTDGRFSGGTSGLSIGHCSPEAAAG
GAIGLVRNGDRIRIDIPNRTINVLVGDEELAQRRAEQDRAGWKPVKPRARKVSVALRAYA
HFATSADKGAVRDLSKLED

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory