SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013819405.1 NCBI__GCF_000214665.1:WP_013819405.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
61% identity, 99% coverage: 1:299/301 of query aligns to 1:301/310 of P9WP55

query
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P9WP55

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K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
N74 (= N74) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 177:181) binding pyridoxal 5'-phosphate
S266 (= S265) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
61% identity, 99% coverage: 1:299/301 of query aligns to 1:301/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K44), S266 (= S265), P293 (≠ H291)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K44), T71 (= T71), S72 (= S72), N74 (= N74), T75 (= T75), Q144 (= Q143), V177 (= V176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (= T181), G222 (= G221), I223 (= I222), S266 (= S265), P293 (≠ H291), D294 (= D292)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
61% identity, 99% coverage: 1:298/301 of query aligns to 1:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K44), S266 (= S265), P293 (≠ H291)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T71), S72 (= S72), I126 (= I126), Q144 (= Q143), F145 (= F144), K215 (≠ H214), G222 (= G221), A225 (= A224), F227 (= F226)
binding pyridoxal-5'-phosphate: K44 (= K44), N74 (= N74), V177 (= V176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (= T181), G222 (= G221), S266 (= S265), P293 (≠ H291), D294 (= D292)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
61% identity, 99% coverage: 1:298/301 of query aligns to 1:300/300 of 2q3cA

query
sites
2q3cA

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L

active site: K44 (= K44), S266 (= S265), P293 (≠ H291)
binding : T71 (= T71), S72 (= S72), G73 (= G73), T75 (= T75), M122 (= M122), Q144 (= Q143), K215 (≠ H214), G222 (= G221), A225 (= A224)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
58% identity, 99% coverage: 1:299/301 of query aligns to 1:304/318 of 4lmaA

query
sites
4lmaA

M

M

A

R

N

I

A

A

I

H

N

D

I

V

T

T

Q

E

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

K

Q

L

L

H

N

R

R

I

I

V

P

P

Q

D

A

D

E

S

G

A

C

-

L

-

G

N

R

V

I

L

V

V

M

K

K

L

L

E

E

S

G

R

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

L

T

A

H

M

M

I

I

Q

N

A

S

A

A

E

E

K

K

S

A

G

G

Y

L

L

I

K

N

A

P

G

E

G

T

S

T

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

I

T

A

A

R

K

G

G

Y

Y

R

R

C

L

T

I

L

L

V

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

Q

A

L

M

L

L

A

K

L

A

Y

Y

G

G

A

A

E

T

I

L

V

E

L

L

T

T

P

P

G

G

S

S

E

Q

G

G

M

M

K

K

G

G

A

A

I

I

S

L

K

R

A

A

H

Q

D

Q

I

I

A

V

A

D

E

S

T

I

-

P

G

G

A

A

F

Y

M

M

P

L

Q

Q

F

F

E

R

N

N

P

P

A

S

N

N

P

P

E

E

V

I

H

H

R

R

Q

L

T

T

A

A

Q

E

E

E

I

I

W

W

S

Q

G

D

T

T

D

E

G

G

Q

Q

I

V

D

D

A

F

F

I

V

V

C

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

A

A

D

E

V

V

I

I

K

K

N

S

R

R

N

K

P

P

D

S

F

F

K

Q

A

V

I

V

A

A

V

V

E

E

P

P

A

F

E

N

S

S

P

P

V

V

I

I

S

S

G

G

V

N

H

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

E

N

V

L

L

D

R

V

T

D

D

L

L

L

I

D

D

G

E

I

V

E

I

L

T

V

V

S

S

T

D

E

E

A

A

F

F

A

Q

M

F

R

G

K

R

R

R

L

L

I

A

Q

K

E

E

G

G

I

L

M

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

S

N

V

L

C

C

A

A

L

I

R

Q

V

L

A

A

A

Q

R

R

-

P

L

E

G

N

V

E

G

G

K

K

T

L

V

I

V

V

T

V

I

I

V

Q

H
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

active site: K46 (= K44), S269 (= S265)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), G181 (= G177), T182 (= T178), G183 (= G179), T185 (= T181), G225 (= G221), S269 (= S265), P296 (≠ H291)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
58% identity, 97% coverage: 8:299/301 of query aligns to 9:302/310 of 5xoqA

query
sites
5xoqA

T

S

Q

E

L

S

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

Q

L

I

H

N

R

R

I

L

V

T

P

A

D

G

D

L

S

S

A

S

N

R

V

V

L

L

V

A

K

K

L

I

E

E

S

G

R

R

N

N

P

P

G

A

G

Y

S

S

V

V

K

K

D

C

R

R

I

I

G

G

L

A

A

A

M

M

I

I

Q

W

A

D

A

A

E

E

K

Q

S

S

G

G

Y

K

L

L

K

K

A

P

G

G

G

M

S

H

I

V

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

F

I

V

A

C

A

A

R

R

G

G

Y

Y

R

K

C

L

T

T

L

L

V

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

Q

M

L

M

L

L

A

K

L

S

Y

F

G

G

A

A

E

D

I

L

V

V

L

L

T

T

P

P

G

G

S

A

E

D

G

G

M
|
M

K

K

G

G

A

A

I

I

S

S

K

K

A

A

H

E

D

E

I

L

A

A

E

Q

T

P

G

G

A

W

F

F

M

I

P

P

Q

Q

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

P

E

A

V

I

H

H

R

V

Q

K

T

T

A

G

Q

P

E

E

I

I

W

W

S

N

G

D

T

T

D

E

G

G

Q

Q

I

V

D

D

A

V

F

F

V

V

C

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

A

A

D

R

V

F

I

L

K

K

N

H

-

E

R

K

N

K

P

H

D

P

F

V

K

H

A

V

I

V

A

A

V

V

E

E

P

P

A

A

E

A

S

S

P

P

V

V

I

L

S

A

G

G

V

P

H

A

S

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

K

D

N

T

L

F

D

D

V

R

D

S

L

V

D

D

G

E

I

I

E

L

L

S

V

V

S

T

T

D

E

D

E

E

A

A

F

I

A

E

M

T

R

A

K

R

R

K

L

L

I

A

Q

M

E

E

G

G

I

I

M

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

S

A

V

M

C

A

A

G

A

A

L

L

R

K

V

V

A

A

R

R

L

P

G

E

V

F

-

A

G

G

K

K

T

T

V

I

V

V

T

T

I

V

V

L

H

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), M123 (= M122), Q144 (= Q143), R218 (≠ P216), H219 (= H217), Q222 (= Q220), G223 (= G221), A226 (= A224)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
56% identity, 99% coverage: 1:299/301 of query aligns to 1:304/310 of 4lmbA

query
sites
4lmbA

M

M

A

L

N

I

A

A

I

R

N

D

I

I

T

T

Q

Q

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

K

Q

L

L

H

N

R

R

I

I

V

P

P

V

D

A

D

E

-

G

-

V

S

K

A

A

N

R

V

I

L

V

V

V

K

K

L

L

E

E

S

S

R

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

L

V

A

S

M

M

I

V

Q

E

A

D

A

A

E

E

K

A

S

A

G

G

Y

L

L

I

K

H

A

P

G

D

G

K

S

T

I

I

-

L

V

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

I

V

A

A

R

K

G

G

Y

Y

R

R

C

L

T

V

L

L

V

T

M

M

P

P

D

E

T

T

M
|
M

S

S

I

L

E

E

R

R

Q

A

L

M

L

L

A

K

L

A

Y

Y

G

G

A

A

E

Q

I

L

V

E

L

L

T

T

P

P

G

G

S

S

E

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

S

T

K

R

A

A

H

E

D

E

I

I

A

V

A

E

E

N

T

T

-

P

G

N

A

A

F

Y

M

S

P

L

Q

Q

Q
|
Q

F
|
F

E

R

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H

H

R

R

Q

E

T

T

A

A

Q

E

E

E

I

I

W

W

S

A

G

D

T

T

D

D

G

G

Q

L

I

V

D

D

A

I

F

V

V

I

C

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

A

A

D

E

V

T

I

I

K

K

N

P

R

R

N

R

P

P

D

Q

F

F

K

Q

A

A

I

I

A

A

V

V

E

E

P

P

A

S

E

N

S

S

P

P

V

V

I

L

S

S

G

G

V

Q

H

P

S

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

A

N

I

L

F

D

R

V

P

D

E

L

L

L

I

D

D

G

E

I

V

E

I

L

I

V

V

S

D

T

D

E

T

E

E

A

A

F

F

A

A

M

Y

R

A

K

R

R

R

L

L

I

A

Q

R

E

Q

E

E

G

G

I

L

M

L

V

S

G

G

I

I

S
|
S

S

A

G

G

A

A

S

A

V

L

C

W

A

A

L

I

R

Q

V

V

A

G

A

K

R

R

L

P

-

E

G

N

V

E

G

D

K

K

T

L

V

I

V

V

T

M

I

I

V

Q

H
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

active site: K46 (= K44), S269 (= S265)
binding cysteine: K46 (= K44), T74 (= T71), S75 (= S72), N77 (= N74), T78 (= T75), M101 (= M98), M125 (= M122), M125 (= M122), Q147 (= Q143), F148 (= F144), Q224 (= Q220), G225 (= G221), G225 (= G221), I226 (= I222), A228 (= A224)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), V180 (= V176), G181 (= G177), T182 (= T178), G183 (= G179), T185 (= T181), G225 (= G221), S269 (= S265), P296 (≠ H291)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
55% identity, 96% coverage: 11:299/301 of query aligns to 12:308/323 of P0ABK5

query
sites
P0ABK5

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

H

N

R

R

I

I

V

-

P

-

D

-

D

G

S

N

A

G

N

R

V

I

L

L

V

A

K

K

L

V

E

E

S

S

R

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

L

A

A

N

M

M

I

I

Q

W

A

D

A

A

E

E

K

K

S

R

G

G

Y

V

L

L

K

K

A

P

G

G

G

V

S

E

I

L

V

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

M

Y

I

V

A

A

R

R

G

G

Y

Y

R

K

C

L

T

T

L

L

V

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

Q

K

L

L

A

K

L

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

P

E

G

G

S

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

S

Q

K

K

A

A

H

E

D

E

I

I

A

V

A

A

E

S

T

N

-

P

-

E

G

K

A

Y

F

L

M

L

P

L

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

V

I

H

H

R

E

Q

K

T

T

A

G

Q

P

E

E

I

I

W

W

S

E

G

D

T

T

D

D

G

G

Q

Q

I

V

D

D

A

V

F

F

V

I

C

A

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

A

S

D

R

V

Y

I

I

K

K

N

G

R

T

-

K

-

G

N

K

P

T

D

D

F

L

K

I

A

S

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

I

I

S

A

G

Q

G

A

V

L

H

A

-

G

-

E

-

I

-

K

-

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

A

N

N

L

L

D

D

V

L

D

K

L

L

L

V

D

D

G

K

I

V

E

I

L

G

V

I

S

T

T

N

E

E

A

A

F

I

A

S

M

T

R

A

K

R

R

R

L

L

I

M

Q

E

E

E

G

G

I

I

M

L

V

A

G

G

I

I

S

S

G

G

A

A

S

A

V

V

C

A

A

A

L

L

R

K

V

L

A

Q

A

E

R

D

L

E

G

S

V

F

-

T

G

N

K

K

T

N

V

I

V

V

T

V

I

I

V

L

H

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

K42 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
55% identity, 96% coverage: 11:299/301 of query aligns to 12:308/323 of P0A1E3

query
sites
P0A1E3

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

H

N

R

R

I

I

V

-

P

-

D

-

D

G

S

N

A

G

N

R

V

I

L

L

V

A

K

K

L

V

E

E

S

S

R

R

N

N

P

P

G

S

G

F

S

S

V

V

K

K

D

C

R

R

I

I

G

G

L

A

A

N

M

M

I

I

Q

W

A

D

A

A

E

E

K

K

S

R

G

G

Y

V

L

L

K

K

A

P

G

G

G

V

S

E

I

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

Y

I

V

A

A

R

R

G

G

Y

Y

R

K

C

L

T

T

L

L

V

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

Q

K

L

L

A

K

L

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

P

E

G

G

S

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

S

Q

K

K

A

A

H

E

D

E

I

I

A

V

A

A

E

S

-

D

-

P

T

Q

G

K

A

Y

F

L

M

L

P

L

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

V

I

H

H

R

E

Q

K

T

T

A

G

Q

P

E

E

I

I

W

W

S

E

G

D

T

T

D

D

G

G

Q

Q

I

V

D

D

A

V

F

F

V

I

C

S

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

A

T

D

R

V

Y

I

I

K

K

N

G

R

T

-

K

-

G

N

K

P

T

D

D

F

L

K

I

A

T

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

I

I

S

A

G

Q

G

A

V

L

H

A

-

G

-

E

-

I

-

K

-

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

V

L

D

K

L

L

L

I

D

D

G

K

I

V

E

V

L

G

V

I

S

T

T

N

E

E

A

A

F

I

A

S

M

T

R

A

K

R

R

R

L

L

I

M

Q

E

E

E

G

G

I

I

M

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

V

C

A

A

A

L

L

R

K

V

L

A

Q

A

E

R

D

L

E

G

S

V

F

-

T

G

N

K

K

T

N

V

I

V

V

T

V

I

I

V

L

H

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

N72 (= N74) binding pyridoxal 5'-phosphate
S273 (= S265) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
55% identity, 96% coverage: 11:299/301 of query aligns to 13:309/321 of 6z4nAAA

query
sites
6z4nAAA

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

H

N

R

R

I

I

V

-

P

-

D

-

D

G

S

N

A

G

N

R

V

I

L

L

V

A

K

K

L

V

E

E

S

S

R

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

L

A

A

N

M

M

I

I

Q

W

A

D

A

A

E

E

K

K

S

R

G

G

Y

V

L

L

K

K

A

P

G

G

G

V

S

E

I

L

V

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

Y

I

V

A

A

R

R

G

G

Y

Y

R

K

C

L

T

T

L

L

V

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

Q

K

L

L

A

K

L

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

P

E

G

G

S

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

S

Q

K

K

A

A

H

E

D

E

I

I

A

V

A

A

E

S

-

D

-

P

T

Q

G

K

A

Y

F

L

M

L

P

L

Q

Q

Q
|
Q

F
|
F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

V

I

H

H

R

E

Q

K

T

T

A

G

Q

P

E

E

I

I

W

W

S

E

G

D

T

T

D

D

G

G

Q

Q

I

V

D

D

A

V

F

F

V

I

C

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

A

T

D

R

V

Y

I

I

K

K

N

G

R

T

-

K

-

G

N

K

P

T

D

D

F

L

K

I

A

T

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

I

I

S

A

G

Q

G

A

V

L

H

A

-

G

-

E

-

I

-

K

-

P

S

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

V

L

D

K

L

L

L

I

D

D

G

K

I

V

E

V

L

G

V

I

S

T

T

N

E

E

A

A

F

I

A

S

M

T

R

A

K

R

R

R

L

L

I

M

Q

E

E

E

G

G

I

I

M

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

V

C

A

A

A

L

L

R

K

V

L

A

Q

A

E

R

D

L

E

G

S

V

F

-

T

G

N

K

K

T

N

V

I

V

V

T

V

I

I

V

L

H
x
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

binding pyridoxal-5'-phosphate: K43 (= K44), N73 (= N74), V177 (= V176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (= T181), G230 (= G221), S274 (= S265), P301 (≠ H291)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K44), T70 (= T71), G72 (= G73), N73 (= N74), T74 (= T75), Q144 (= Q143), F145 (= F144), Q229 (= Q220), G230 (= G221), I231 (= I222), A233 (= A224)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
55% identity, 98% coverage: 4:299/301 of query aligns to 14:312/323 of 4aecA

query
sites
4aecA

A

A

I

D

N

N

I

V

T

S

Q

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

H

N

R

S

I

I

V

A

P

K

D

G

D

C

S

V

A

A

N

N

V

I

L

A

V

A

K

K

L

L

E

E

S

I

R

M

N

E

P

P

G

C

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

L

Y

A

S

M

M

I

V

Q

T

A

D

A

A

E

E

K

Q

S

K

G

G

Y

F

L

I

K

S

A

P

G

G

G

K

S

S

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

A

A

A

S

R

R

G

G

Y

Y

R

R

C

L

T

I

L

L

V

T

M

M

P

P

D

A

T

S

M

M

S

S

I

M

E

E

R

R

Q

V

L

L

A

K

L

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

A

E

K

G

G

M

M

K

T

G

G

A

A

I

V

S

Q

K

K

A

A

H

E

D

E

I

I

A

L

A

K

E

N

T

T

-

P

G

D

A

A

F

Y

M

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

I

W

W

S

D

G

D

T

T

D

K

G

G

Q

K

I

V

D

D

A

I

F

F

V

V

C

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

A

G

D

R

V

F

I

I

K

K

N

E

R

K

N

N

P

P

D

K

F

T

K

Q

A

V

I

I

A

G

V

V

E

E

P

P

A

T

E

E

S

S

P

D

V

I

I

L

S

S

G

G

V

K

H

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

K

N

N

L

L

D

D

V

Q

D

K

L

I

L

M

D

D

G

E

I

V

E

I

L

A

V

I

S

S

T

S

E

E

A

A

F

I

A

E

M

T

R

A

K

K

R

Q

L

L

I

A

Q

L

E

K

E

E

G

G

I

L

M

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

A

C

A

A

A

L

I

R

K

V

V

A

A

A

K

R

R

L

P

-

E

G

N

V

A

G

G

K

K

T

L

V

I

V

A

T

V

I

V

V

F

H
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

active site: K54 (= K44), S277 (= S265)
binding pyridoxal-5'-phosphate: K54 (= K44), N85 (= N74), I188 (≠ V176), G189 (= G177), T190 (= T178), G191 (= G179), G192 (= G180), T193 (= T181), G233 (= G221), S277 (= S265), P304 (≠ H291)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
54% identity, 97% coverage: 11:301/301 of query aligns to 11:309/322 of 1d6sA

query
sites
1d6sA

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

H

N

R

R

I

I

V

-

P

-

D

-

D

G

S

N

A

G

N

R

V

I

L

L

V

A

K

K

L

V

E

E

S

S

R

R

N

N

P

P

G

S

G

F

S

S

V

V

K
x
A

D

C

R

R

I

I

G

G

L

A

A

N

M

M

I

I

Q

W

A

D

A

A

E

E

K

K

S

R

G

G

Y

V

L

L

K

K

A

P

G

G

G

V

S

E

I

L

V

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

Y

I

V

A

A

R

R

G

G

Y

Y

R

K

C

L

T

T

L

L

V

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

Q

K

L

L

A

K

L

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

P

E

G

G

S

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

S

Q

K

K

A

A

H

E

D

E

I

I

A

V

A

A

E

S

-

D

-

P

T

Q

G

K

A

Y

F

L

M

L

P

L

Q

Q

Q
|
Q

F
|
F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

V

I

H

H

R

E

Q

K

T

T

A

G

Q

P

E

E

I

I

W

W

S

E

G

D

T

T

D

D

G

G

Q

Q

I

V

D

D

A

V

F

F

V

I

C

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

A

T

D

R

V

Y

I

I

K

K

N

G

R

T

-

K

-

G

N

K

P

T

D

D

F

L

K

I

A

T

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

I

I

S

A

G

Q

G

A

V

L

H

A

-

G

-

E

-

I

-

K

-

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

V

L

D

K

L

L

L

I

D

D

G

K

I

V

E

V

L

G

V

I

S

T

T

N

E

E

A

A

F

I

A

S

M

T

R

A

K

R

R

R

L

L

I

M

Q

E

E

E

G

G

I

I

M

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

V

C

A

A

A

L

L

R

K

V

L

A

Q

A

E

R

D

L

E

G

S

V

F

-

T

G

N

K

K

T

N

V

I

V

V

T

V

I

I

V

L

H
x
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

M

T

S

A

active site: A41 (≠ K44), G228 (= G221)
binding methionine: T68 (= T71), N69 (≠ S72), N71 (= N74), T72 (= T75), Q142 (= Q143), F143 (= F144), G176 (= G177), G228 (= G221)
binding pyridoxal-5'-phosphate: N71 (= N74), G176 (= G177), T177 (= T178), G178 (= G179), T180 (= T181), G228 (= G221), S272 (= S265), P299 (≠ H291)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
55% identity, 98% coverage: 4:299/301 of query aligns to 4:302/309 of 7n2tA

query
sites
7n2tA

A

A

I

K

N

D

I

V

T

T

Q

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

H

N

R

R

I

V

V

V

P

D

D

G

D

C

S

V

A

A

N

R

V

V

L

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

P

P

G

C

G

S

S

S

V

V

K

K

D

D

R

R

I

I

G

G

L

Y

A

S

M

M

I

I

Q

S

A

D

A

A

E

E

K

N

S

K

G

G

Y

L

L

I

K

T

A

P

G

G

G

E

S

S

I

V

-

L

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

A

A

R

K

G

G

Y

Y

R

R

C

L

T

I

L

I

V

C

M

M

P

P

D

A

T

S

M

M

S

S

I

L

E

E

R

R

Q

T

L

I

L

L

A

R

L

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

A

E

R

G

G

M

M

K

K

G

G

A

A

I

V

S

Q

K

K

A

A

H

E

D

E

I

I

A

K

A

A

E

K

T

T

-

P

G

N

A

S

F

Y

M

I

P

L

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

I

W

W

S

R

G

G

T

S

D

G

G

G

Q

K

I

I

D

D

A

A

F

L

V

V

C

S

G

G

V

I

G

G

T

T

G

G

T

T

I

V

T

T

G

G

V

A

A

G

D

K

V

Y

I

L

K

K

N

E

R

Q

N

N

P

P

D

N

F

I

K

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

I

L

S

S

G

G

V

K

H

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

V

L

L

D

D

V

V

D

N

L

L

D

D

G

E

I

V

E

I

L

Q

V

V

S

S

T

S

E

E

A

S

F

I

A

E

M

T

R

A

K

K

R

L

L

L

I

A

Q

L

E

K

E

E

G

G

I

L

M

L

V

V

G

G

I

I

S

S

G

G

A

A

S

A

V

A

C

A

A

A

L

I

R
|
R

V

I

A

A

A

K

R
|
R

-

P

L

E

G

N

V

A

G

G

K

K

T

L

V

I

V

V

T

A

I

V

V

F

H

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

binding phosphate ion: R277 (= R275), R281 (= R279)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
53% identity, 98% coverage: 4:299/301 of query aligns to 6:304/322 of P47998

query
sites
P47998

A

A

I

K

N

D

I

V

T

T

Q

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

H

N

R

N

I

V

V

A

P

E

D

G

D

C

S

V

A

G

N

R

V

V

L

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

L

F

A

S

M

M

I

I

Q

S

A

D

A

A

E

E

K

K

S

K

G

G

Y

L

L

I

K

K

A

P

G

G

G

E

S

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

I

T

A

A

R

K

G

G

Y

Y

R

K

C

L

T

I

L

I

V

T

M

M

P

P

D

A

T

S

M

M

S

S

I

T

E

E

R

R

Q

I

L

I

L

L

A

L

L

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

S

A

K

K

A

A

H

E

D

E

I

I

A

L

A

A

E

K

T

T

-

P

G

N

A

G

F

Y

M

M

P

L

Q

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H
|
H

R

Y

Q

E

T

T

A
x
G

Q

P

E

E

I

I

W

W

S

K

G

G

T

T

D

G

G

G

Q

K

I

I

D

D

A

G

F

F

V

V

C

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

A

G

D

K

V

Y

I

L

K

K

N

E

R

Q

N

N

P

A

D

N

F

V

K

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

I

L

S

S

G

G

V
x
K

H

P

S

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

N

V

L

L

D

N

V

V

D

D

L

L

L

I

D

D

G

E

I

V

E

V

L

Q

V

V

S

S

T

S

E

D

E

E

A

S

F

I

A

D

M

M

R

A

K

R

R

Q

L

L

I

A

Q

L

E

K

E

E

G

G

I

L

M

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

A

C

A

A

A

L

I

R

K

V

L

A

A

A

Q

R

R

-

P

L

E

G

N

V

A

G

G

K

K

T

L

V

F

V

V

T

A

I

I

V

F

H

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

K46 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T71) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S72) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N74) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T75) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q143) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H153) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A158) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 177:181) binding pyridoxal 5'-phosphate
T182 (= T178) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T181) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ V213) mutation to A: Impaired interaction with SAT1.
H221 (= H217) mutation to A: Impaired interaction with SAT1.
K222 (= K218) mutation to A: Impaired interaction with SAT1.
S269 (= S265) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
53% identity, 98% coverage: 4:299/301 of query aligns to 4:302/320 of 2isqA

query
sites
2isqA

A

A

I

K

N

D

I

V

T

T

Q

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

H

N

R

N

I

V

V

A

P

E

D

G

D

C

S

V

A

G

N

R

V

V

L

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

L

F

A

S

M

M

I

I

Q

S

A

D

A

A

E

E

K

K

S

K

G

G

Y

L

L

I

K

K

A

P

G

G

G

E

S

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

I

T

A

A

R

K

G

G

Y

Y

R

K

C

L

T

I

L

I

V

T

M

M

P

P

D

A

T

S

M

M

S

S

I

T

E

E

R

R

Q

I

L

I

L

L

A

L

L

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

A

E

K

G
|
G

M
|
M

K
|
K

G

G

A

A

I

I

S

A

K

K

A

A

H

E

D

E

I

I

A

L

A

A

E

K

T

T

-

P

G

N

A

G

F

Y

M

M

P

L

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

I

W

W

S

K

G

G

T

T

D

G

G

G

Q

K

I

I

D

D

A

G

F

F

V

V

C

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

A

G

D

K

V

Y

I

L

K

K

N

E

R

Q

N

N

P

A

D

N

F

V

K

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

I

L

S

S

G

G

V

K

H

P

S
x
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

N

V

L

L

D

N

V

V

D

D

L

L

L

I

D

D

G

E

I

V

E

V

L

Q

V

V

S

S

T

S

E

D

E

E

A

S

F

I

A

D

M

M

R

A

K

R

R

Q

L

L

I

A

Q

L

E

K

E

E

G

G

I

L

M

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

A

C

A

A

A

L

I

R

K

V

L

A

A

A

Q

R

R

-

P

L

E

G

N

V

A

G

G

K

K

T

L

V

F

V

V

T

A

I

I

V

F

H
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

active site: K44 (= K44), S267 (= S265)
binding pyridoxal-5'-phosphate: K44 (= K44), N75 (= N74), G177 (= G175), G179 (= G177), T180 (= T178), G181 (= G179), T183 (= T181), G223 (= G221), S267 (= S265), P294 (≠ H291)
binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), G122 (= G121), M123 (= M122), K124 (= K123), G217 (≠ S215), P218 (= P216), H219 (= H217), Q222 (= Q220), G223 (= G221)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
54% identity, 96% coverage: 11:299/301 of query aligns to 7:297/302 of 2efyA

query
sites
2efyA

I

I

G

G

N

K

T

T

P

P

L

V

V

V

K

R

L

L

H

A

R

K

I

V

V

V

P

E

D

P

D

D

S

M

A

A

N

E

V

V

L

W

V

V

K

K

L

L

E

E

S

G

R

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

L

W

A

Y

M

M

I

I

Q

K

A

D

A

A

E

E

K

E

S

R

G

G

Y

I

L

L

K

R

A

P

G

G

G

S

S

G

-

Q

-

V

I

I

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

A

A

A

S

R

R

G

G

Y

Y

R

R

C

L

T

I

L

L

V

T

M

M

P

P

D

A

T

Q

M

M

S

S

I

E

E

E

R

R

R

K

Q

R

L

V

L

L

A

K

L

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

E

E

R

G

R

M

M

K

L

G

A

A

A

I

R

S

E

K

E

A

A

H

L

D

R

I

L

A

K

A

E

E

E

T

L

G

G

A

A

F

F

M

M

P

P

Q

D

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

V

E

R

V

A

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

L

W

Y

S

E

G

A

T

L

D

E

G

G

Q

R

I

I

D

D

A

A

F

F

V

V

C
x
Y

G

G

V

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

A

G

D

R

V

Y

I

L

K

K

N

E

R

R

N

I

P

P

D

H

F

V

K

K

A

V

I

I

A

A

V

V

E
|
E

P

P

A

A

E

R

S
|
S

P

N

V

V

I

L

S

S

G

G

V

K

H

M

S

G

P

Q

H

H

K

G

I

F

Q

Q

G
|
G

I
x
M

G

G

A
x
P

G

G

F

F

V

I

P

P

K

E

N

N

L

L

D

D

V

L

D

S

L

L

D

D

G

G

I

V

E

I

L

Q

V

V

S

W

T

E

E

E

E

D

A

A

F

F

A

P

M

L

R

A

K

R

R

R

L

L

I

A

Q

R

E

E

G

G

I

L

M

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

S

I

V

V

C

W

A

A

L

L

R

Q

V

V

A

A

A

R

R

E

L

L

G

G

V

P

G

G

K

K

T

R

V

V

V

A

T

C

I

I

V

S

H
x
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

active site: K40 (= K44), S70 (= S72), E200 (= E202), S204 (= S206), S263 (= S265)
binding 5-oxohexanoic acid: T69 (= T71), G71 (= G73), T73 (= T75), Q141 (= Q143), G175 (= G177), G219 (= G221), M220 (≠ I222), P222 (≠ A224)
binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ C174), G175 (= G177), T176 (= T178), G177 (= G179), T179 (= T181), G219 (= G221), S263 (= S265), P289 (≠ H291), D290 (= D292)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
54% identity, 96% coverage: 11:299/301 of query aligns to 7:297/302 of 2ecqA

query
sites
2ecqA

I

I

G

G

N

K

T

T

P

P

L

V

V

V

K

R

L

L

H

A

R

K

I

V

V

V

P

E

D

P

D

D

S

M

A

A

N

E

V

V

L

W

V

V

K

K

L

L

E

E

S

G

R

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

L

W

A

Y

M

M

I

I

Q

K

A

D

A

A

E

E

K

E

S

R

G

G

Y

I

L

L

K

R

A

P

G

G

G

S

S

G

-

Q

-

V

I

I

V

V

E

E

P

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

A

A

A

S

R

R

G

G

Y

Y

R

R

C

L

T

I

L

L

V

T

M

M

P

P

D

A

T

Q

M

M

S

S

I

E

E

E

R

R

R

K

Q

R

L

V

L

L

A

K

L

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

E

E

R

G

R

M

M

K

L

G

A

A

A

I

R

S

E

K

E

A

A

H

L

D

R

I

L

A

K

A

E

E

E

T

L

G

G

A

A

F

F

M

M

P

P

Q

D

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

V

E

R

V

A

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

L

W

Y

S

E

G

A

T

L

D

E

G

G

Q

R

I

I

D

D

A

A

F

F

V

V

C
x
Y

G
|
G

V

S

G
|
G

T
|
T

G

G

T
|
T

I

I

T

T

G

G

V

V

A

G

D

R

V

Y

I

L

K

K

N

E

R

R

N

I

P

P

D

H

F

V

K

K

A

V

I

I

A

A

V

V

E
|
E

P

P

A

A

E

R

S
|
S

P

N

V

V

I

L

S

S

G

G

V

K

H

M

S

G

P

Q

H

H

K

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

V

I

P

P

K

E

N

N

L

L

D

D

V

L

D

S

L

L

D

D

G

G

I

V

E

I

L

Q

V

V

S

W

T

E

E

E

E

D

A

A

F

F

A

P

M

L

R

A

K

R

R

R

L

L

I

A

Q

R

E

E

G

G

I

L

M

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

S

I

V

V

C

W

A

A

L

L

R

Q

V

V

A

A

A

R

R

E

L

L

G

G

V

P

G

G

K

K

T

R

V

V

V

A

T

C

I

I

V

S

H
x
P

D

D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

active site: K40 (= K44), S70 (= S72), E200 (= E202), S204 (= S206), S263 (= S265)
binding (3s)-3-hydroxybutanoic acid: K40 (= K44), G71 (= G73), T73 (= T75), Q141 (= Q143), G219 (= G221)
binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ C174), G173 (= G175), G175 (= G177), T176 (= T178), T179 (= T181), G219 (= G221), S263 (= S265), P289 (≠ H291)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
54% identity, 96% coverage: 11:299/301 of query aligns to 7:297/302 of 2ecoA

query
sites
2ecoA

I

I

G

G

N

K

T

T

P

P

L

V

V

V

K

R

L

L

H

A

R

K

I

V

V

V

P

E

D

P

D

D

S

M

A

A

N

E

V

V

L

W

V

V

K

K

L

L

E

E

S

G

R

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

L

W

A

Y

M

M

I

I

Q

K

A

D

A

A

E

E

K

E

S

R

G

G

Y

I

L

L

K

R

A

P

G

G

G

S

S

G

-

Q

-

V

I

I

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

A

A

A

S

R

R

G

G

Y

Y

R

R

C

L

T

I

L

L

V

T

M

M

P

P

D

A

T

Q

M

M

S

S

I

E

E

E

R

R

R

K

Q

R

L

V

L

L

A

K

L

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

E

E

R

G

R

M

M

K

L

G

A

A

A

I

R

S

E

K

E

A

A

H

L

D

R

I

L

A

K

A

E

E

E

T

L

G

G

A

A

F

F

M

M

P

P

Q

D

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

V

E

R

V

A

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

L

W

Y

S

E

G

A

T

L

D

E

G

G

Q

R

I

I

D

D

A

A

F

F

V

V

C
x
Y

G

G

V

S

G
|
G

T
|
T

G

G

T
|
T

I

I

T

T

G

G

V

V

A

G

D

R

V

Y

I

L

K

K

N

E

R

R

N

I

P

P

D

H

F

V

K

K

A

V

I

I

A

A

V

V

E
|
E

P

P

A

A

E

R

S
|
S

P

N

V

V

I

L

S

S

G

G

V

K

H

M

S

G

P

Q

H

H

K

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

V

I

P

P

K

E

N

N

L

L

D

D

V

L

D

S

L

L

D

D

G

G

I

V

E

I

L

Q

V

V

S

W

T

E

E

E

E

D

A

A

F

F

A

P

M

L

R

A

K

R

R

R

L

L

I

A

Q

R

E

E

G

G

I

L

M

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

S

I

V

V

C

W

A

A

L

L

R

Q

V

V

A

A

A

R

R

E

L

L

G

G

V

P

G

G

K

K

T

R

V

V

V

A

T

C

I

I

V

S

H
x
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

active site: K40 (= K44), S70 (= S72), E200 (= E202), S204 (= S206), S263 (= S265)
binding 4-methyl valeric acid: K40 (= K44), T69 (= T71), G71 (= G73), T73 (= T75), Q141 (= Q143), G175 (= G177), T176 (= T178), G219 (= G221)
binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ C174), G175 (= G177), T176 (= T178), T179 (= T181), G219 (= G221), S263 (= S265), P289 (≠ H291), D290 (= D292)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
53% identity, 98% coverage: 4:299/301 of query aligns to 4:302/320 of 1z7yA

query
sites
1z7yA

A

A

I

K

N

D

I

V

T

T

Q

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

H

N

R

N

I

V

V

A

P

E

D

G

D

C

S

V

A

G

N

R

V

V

L

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

P

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

I

I

G

G

L

F

A

S

M

M

I

I

Q

S

A

D

A

A

E

E

K

K

S

K

G

G

Y

L

L

I

K

K

A

P

G

G

G

E

S

S

I

V

-

L

V

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

I

T

A

A

R

K

G

G

Y

Y

R

K

C

L

T

I

L

I

V

T

M

M

P

P

D

A

T

S

M

M

S

S

I

T

E

E

R

R

Q

I

L

I

L

L

A

L

L

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

P

D

G

P

S

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

S

A

K

K

A

A

H

E

D

E

I

I

A

L

A

A

E

K

T

T

-

P

G

N

A

G

F

Y

M

M

P

L

Q

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

I

W

W

S

K

G

G

T

T

D

G

G

G

Q

K

I

I

D

D

A

G

F

F

V

V

C

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

A

G

D

K

V

Y

I

L

K

K

N

E

R

Q

N

N

P

A

D

N

F

V

K

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

I

L

S

S

G

G

V

K

H

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

N

V

L

L

D

N

V

V

D

D

L

L

L

I

D

D

G

E

I

V

E

V

L

Q

V

V

S

S

T

S

E

D

E

E

A

S

F

I

A

D

M

M

R

A

K

R

R

Q

L

L

I

A

Q

L

E

K

E

E

G

G

I

L

M

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

V

A

C

A

A

A

L

I

R

K

V

L

A

A

A

Q

R

R

-

P

L

E

G

N

V

A

G

G

K

K

T

L

V

F

V

V

T

A

I

I

V

F

H
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

active site: A44 (≠ K44), S267 (= S265)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G73), N75 (= N74), T76 (= T75), Q145 (= Q143), I178 (≠ V176), G179 (= G177), T180 (= T178), G181 (= G179), T183 (= T181), G223 (= G221), S267 (= S265), P294 (≠ H291), S295 (≠ D292)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
54% identity, 98% coverage: 4:299/301 of query aligns to 76:374/392 of P47999

query
sites
P47999

A

A

I

D

N

N

I

A

T

A

Q

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

H

N

R

N

I

V

V

V

P

K

D

G

D

C

S

V

A

A

N

S

V

V

L

A

V

A

K

K

L

L

E

E

S

I

R

M

N

E

P

P

G

C

S

S

V

V

K

K

D

D

R

R

I

I

G

G

L

Y

A

S

M

M

I

I

Q

T

A

D

A

A

E

E

K

E

S

K

G

G

Y

L

L

I

K

T

A

P

G

G

G

K

S

S

I

V

-

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

A

A

A

S

R

K

G

G

Y

Y

R

K

C

L

T

I

L

L

V

T

M

M

P

P

D

A

T

S

M

M

S

S

I

L

E

E

R

R

Q

V

L

L

A

R

L

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

E

G

P

S

A

E

K

G

G

M

M

K

T

G

G

A

A

I

I

S

Q

K

K

A

A

H

E

D

E

I

I

A

L

A

K

E

K

T

T

-

P

G

N

A

S

F

Y

M

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

E

K

V

I

H

H

R

Y

Q

E

T

T

A

G

Q

P

E

E

I

I

W

W

S

E

G

D

T

T

D

R

G

G

Q

K

I

I

D

D

A

I

F

L

V

V

C

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

A

G

D

R

V

F

I

I

K

K

N

E

R

R

N

K

P

P

D

E

F

L

K

K

A

V

I

I

A

G

V

V

E

E

P

P

A

T

E

E

S

S

P

A

V

I

I

L

S

S

G

G

V

K

H

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

K

K

N

N

L

L

D

D

V

L

D

A

L

I

L

V

D

D

G

E

I

Y

E

I

L

A

V

I

S

S

T

S

E

E

A

A

F

I

A

E

M

T

R

S

K

K

R

Q

L

L

I

A

Q

L

E

Q

E

E

G

G

I

L

M

L

V

V

G

G

I

I

S

S

G

G

A

A

S

A

V

A

C

A

A

A

L

I

R

Q

V

V

A

A

A

K

R

R

-

P

L

E

G

N

V

A

G

G

K

K

T

L

V

I

V

A

T

V

I

V

V

F

H

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

Sites not aligning to the query:

61 modified: N-acetylalanine

Query Sequence

>WP_013819405.1 NCBI__GCF_000214665.1:WP_013819405.1
MANAINITQLIGNTPLVKLHRIVPDDSANVLVKLESRNPGGSVKDRIGLAMIQAAEKSGY
LKAGGSIVEPTSGNTGIALAMIAAARGYRCTLVMPDTMSIERRQLLALYGAEIVLTPGSE
GMKGAISKAHDIAAETGAFMPQQFENPANPEVHRQTTAQEIWSGTDGQIDAFVCGVGTGG
TITGVADVIKNRNPDFKAIAVEPAESPVISGGVHSPHKIQGIGAGFVPKNLDVDLLDGIE
LVSTEEAFAMRKRLIQEEGIMVGISSGASVCAALRVAARLGVGKTVVTIVHDTGERYLSM
S

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory