SitesBLAST

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
30% identity, 92% coverage: 12:265/275 of query aligns to 18:273/283 of P19938

query
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P19938

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Y32 (≠ F26) binding substrate
R51 (= R45) binding pyridoxal 5'-phosphate
R99 (= R90) binding substrate
H101 (≠ Y92) binding substrate
K146 (= K140) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
E178 (= E173) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
L202 (≠ I197) mutation to A: Inactivates enzyme.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
30% identity, 92% coverage: 12:265/275 of query aligns to 17:272/277 of 3daaA

query
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3daaA

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active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ I197)
binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F26), R50 (= R45), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (= S177), L201 (≠ I197), G203 (= G199), I204 (= I200), T205 (≠ L201), S240 (≠ N236), T241 (≠ S237)

2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
30% identity, 92% coverage: 12:265/275 of query aligns to 17:272/277 of 2daaA

query
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2daaA

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active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ I197)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F26), V33 (≠ T28), R50 (= R45), R98 (= R90), H100 (≠ Y92), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (= S177), N182 (= N178), L201 (≠ I197), G203 (= G199), I204 (= I200), T205 (≠ L201), T241 (≠ S237)

1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
30% identity, 92% coverage: 12:265/275 of query aligns to 17:272/277 of 1daaA

query
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1daaA

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active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ I197)
binding pyridoxal-5'-phosphate: R50 (= R45), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (= S177), L201 (≠ I197), G203 (= G199), I204 (= I200), T205 (≠ L201), S240 (≠ N236), T241 (≠ S237)

3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
30% identity, 92% coverage: 12:265/275 of query aligns to 17:272/280 of 3lqsA

query
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3lqsA

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W

-

R

-

L

-

A

D

I

I

N

R

P

W

G

L

F

R

A

C

G

D

I

I

K
|
K

H

S

M

L

N

N

R

L

L

L

E

G

Q

A

V

V

M

L

A

A

R

K

A

Q

E

E

W

A

Q

H

H

E

D

K

T

G

V

C

Q

Y

E

E

G

A

L

I

M

L

F

H

D

-

S

R

D

N

R

T

I

V

V

T

E
|
E

G

G

T

S

M
x
S

S
|
S

N
|
N

L

V

F

F

F

G

V

I

K

K

D

D

G

G

I

I

L

L

H

Y

T

T

P

H

L

P

L

A

V

N

N

N

C

M

G

I

I
x
L

A

K

G
|
G

I
|
I

L
x
T

R

R

Q

D

I

V

V

V

I

I

E

A

F

C

A

A

V

N

R

E

N

I

G

N

L

M

A

P

L

V

S

K

E

E

G

I

H

P

F

F

S

T

K

T

Q

H

N

E

V

A

L

L

E

K

A

M

D

D

E

E

V

L

F

F

V

V

T

T

N
x
S

S
x
T

V
x
T

I

S

G

E

I

I

W

T

P

P

V

V

K

I

Q

E

L

I

E

D

K

G

Q

K

C

L

F

I

C

R

V

D

G

G

R

K

V

V

-

G

-

E

-

W

T

T

R

R

A

K

I

L

Q

Q

Q

K

W

Q

Y

F

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ I197)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ T28), R50 (= R45), E177 (= E173), S180 (≠ M176), S181 (= S177), N182 (= N178), L201 (≠ I197), G203 (= G199), I204 (= I200), T205 (≠ L201), S240 (≠ N236), T241 (≠ S237), T242 (≠ V238)

1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
30% identity, 92% coverage: 12:265/275 of query aligns to 17:272/282 of 1a0gB

query
sites
1a0gB

V

I

D

D

V

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

I

V

A

K

V

V

C

Y

N

N

G

G

R

E

P

M

L

F

F

T

L

V

Q

N

R

E

H

H

L

I

D

D

R
|
R

L

L

L

Y

K

A

G

S

C

A

R

E

R

K

L

I

H

R

I

I

P

T

P

I

P

P

D

Y

L

T

S

K

-

D

-

K

-

F

-

H

-

Q

L

L

E

H

S

E

-

L

-

V

E

E

A

K

R

N

Q

E

L

L

S

N

A

T

G

G

A

H

D

-

V

-

A

-

V

-

L

I

K

Y

L

F

I

Q

L

V

T

T

R

R

G

G

S

T

G

S

G

P

R

R

G

A

Y

H

R

Q

Q

F

P

P

E

E

Q

N

I

T

V

V

P

K

T

P

R

V

L

I

F

I

S

G

L

Y

H

T

P

K

S

E

P

N

N

P

Y

R

P

P

D

L

C

E

Y

N

Q

L

T

E

D

K

G

G

I

V

V

K

A

A

R

T

F

F

C

V

E

E

W

-

R

-

L

-

A

D

I

I

N

R

P

W

G

L

F

R

A

C

G

D

I

I

K
|
K

H

S

M

L

N

N

R

L

L

L

E

G

Q

A

V

V

M

L

A

A

R

K

A

Q

E

E

W

A

Q

H

H

E

D

K

T

G

V

C

Q

Y

E

E

G

A

L

I

M

L

F

H

D

-

S

R

D

N

R

T

I

V

V

T

E
|
E

G

G

T

S

M
x
S

S
|
S

N

N

L

V

F

F

F

G

V

I

K

K

D

D

G

G

I

I

L

L

H

Y

T

T

P

H

L

P

L

A

V

N

N

N

C

M

G

I

I
x
A

A

K

G
|
G

I
|
I

L
x
T

R

R

Q

D

I

V

V

V

I

I

E

A

F

C

A

A

V

N

R

E

N

I

G

N

L

M

A

P

L

V

S

K

E

E

G

I

H

P

F

F

S

T

K

T

Q

H

N

E

V

A

L

L

E

K

A

M

D

D

E

E

V

L

F

F

V

V

T

T

N
x
S

S
x
T

V

T

I

S

G

E

I

I

W

T

P

P

V

V

K

I

Q

E

L

I

E

D

K

G

Q

K

C

L

F

I

C

R

V

D

G

G

R

K

V

V

-

G

-

E

-

W

T

T

R

R

A

K

I

L

Q

Q

Q

K

W

Q

Y

F

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), A201 (≠ I197)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R45), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (= S177), G203 (= G199), I204 (= I200), T205 (≠ L201), S240 (≠ N236), T241 (≠ S237)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
30% identity, 94% coverage: 7:265/275 of query aligns to 14:278/290 of 5e25A

query
sites
5e25A

E

E

S

S

R

Q

H

A

C

K

V

V

D

S

V

V

S

F

D

D

R

H

G

G

F

F

Q

L

Y

Y

G

G

D

D

G

G

L

V

F
|
F

E

E

T
x
G

I

I

A

R

V

A

C

Y

N

N

G

G

R

K

P

V

L

F

F

K

L

L

Q

Y

R

E

H

H

L

I

D

D

R
|
R

L

L

L

Y

K

D

G

C

C

A

R

R

-

V

-

I

R

D

L

L

H

K

I

I

P

P

P

L

P

S

D

K

L

E

S

E

L

F

L

A

E

E

S

A

E

I

A

L

R

E

Q

T

L

L

-

R

S

R

A

N

G

N

A

L

D

R

V

D

A

A

V
x
Y

L

I

K

R

L

P

I

I

L

V

T

T

R

R

G

G

S

A

G

G

G

D

R

L

G

G

Y

L

R

D

Q

P

P

R

E

K

Q

C

I

P

V

S

P

P

T

N

R

V

L

I

F

I

S

I

L

T

H

K

P

P

-

W

S

G

P

K

N

L

Y

Y

P

G

D

D

C

L

Y

Y

Q

E

T

K

D

G

G

L

I

K

V

A

A

I

R

T

F

V

C

A

E

I

W

R

R

R

L

N

A

A

I

I

N

D

P

S

G

L

F

P

A

P

G

N

I

I

K
|
K

H

S

M

L

N

N

R
x
Y

L

L

E

N

Q

N

V

I

M

L

A

A

R

K

A

I

E

E

W

A

Q

N

H

A

D

K

T

G

V

G

Q

D

E

E

G

A

L

I

M

F

F

L

D

D

S

H

D

N

D

G

R

Y

I

I

V

S

E
|
E

G

G

T

S

M
x
G

S
x
D

N

N

L

I

F

F

F

I

V

V

K

K

D

N

G

G

I

T

L

I

H

T

T

T

P

P

L

P

L

T

V

L

N

N

C

-

G

N

I
x
L

A

K

G
|
G

I
|
I

L
x
T

R

R

Q

Q

I

V

V

V

I

I

E

E

F

L

A

I

V

N

R

E

N

L

G

E

L

I

A

P

L

F

S

R

E

E

G

A

H

N

F

I

S

G

K

L

Q

F

N

D

V

L

L

Y

E

S

A

A

D

D

E

E

V

I

F

F

V

V

T

T

N
x
G

S
x
T

V
x
A

I

A

G

E

I

I

W

A

P

P

V

V

K

T

Q

Y

L

I

E

D

K

G

Q

R

C

T

F

V

C

G

-

N

-

G

-

K

V

P

G

G

R

K

V

V

T

T

R

K

A

M

I

L

Q

M

Q

E

W

K

Y

F

active site: F33 (= F26), G35 (≠ T28), K151 (= K140), E184 (= E173), L207 (≠ I197)
binding 2-oxoglutaric acid: Y88 (≠ V78), K151 (= K140), T247 (≠ S237), A248 (≠ V238)
binding pyridoxal-5'-phosphate: R52 (= R45), K151 (= K140), Y155 (≠ R144), E184 (= E173), G187 (≠ M176), D188 (≠ S177), L207 (≠ I197), G209 (= G199), I210 (= I200), T211 (≠ L201), G246 (≠ N236), T247 (≠ S237)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
29% identity, 95% coverage: 7:266/275 of query aligns to 13:278/290 of 5mr0D

query
sites
5mr0D

E

E

S

N

R

E

H

A

C

K

V

V

D

S

V

I

S

F

D

D

R

H

G

G

F

F

Q

L

Y

Y

G

G

D

D

G

G

L

V

F
|
F

E

E

T
x
G

I

I

A

R

V

A

C

Y

N

N

G

G

R

R

P

V

L

F

F

R

L

L

Q

K

R

E

H

H

L

I

D

D

R
|
R

L

L

L

Y

K

D

G

S

C

A

R

K

R

A

-

I

-

D

L

L

H

E

I

I

P

P

P

I

P

T

D

K

L

E

S

E

L

F

L

M

E

E

S

I

E

I

A

L

R

E

Q

T

L

L

-

R

S

K

A

N

G

N

A

L

D

R

V

D

A

A

V

Y

L

I

K

R

L

P

I

I

L

V

T

T

R

R

G

G

S

I

G

G

G

D

R

L

G
|
G

Y
x
L

-

D

-

P

-

R

-

K

-

C

R

Q

Q

N

P

P

E

S

Q

I

I

I

V

V

P

I

T

T

R

K

L

P

F

W

S

G

L

K

H

L

P

-

S

-

P

-

N

-

Y

Y

P

G

D

D

C

L

Y

Y

Q

E

T

K

D

G

G

L

I

T

V

A

A

I

R

T

F

V

C

A

E

V

W

R

R

R

L

N

A

S

I

F

N

D

P

A

G

L

F

P

A

P

G

N

I

I

K
|
K

H

S

M

L

N

N

R
x
Y

L

L

E

N

Q

N

V

I

M

L

A

A

R

K

A

I

E

E

W

A

Q

N

H

A

D

K

T

G

V

G

Q

D

E

E

G

A

L

I

M

F

F

L

D

D

S

R

D

N

D

G

R

Y

I

V

V

S

E
|
E

G

G

T

S

M
x
G

S
x
D

N

N

L

I

F

F

F

V

V

V

K

K

D

N

G

G

I

A

L

I

H

T

T

T

P

P

L

P

L

T

V

I

N

N

C

-

G

N

I
x
L

A

R

G

G

I
|
I

L
x
T

R

R

Q

E

I

A

V

V

I

I

E

E

F

I

A

I

V

N

R

R

N

L

G

G

L

I

A

P

L

F

S

K

E

E

G

T

H

N

F

I

S

G

K

L

Q

Y

N

D

V

L

L

Y

E

T

A

A

D

D

E

E

V

V

F

F

V

V

T

T

N
x
G

S
x
T

V

A

I

A

G

E

I

I

W

A

P

P

V

I

K

V

Q

V

L

I

E

D

K

G

Q

R

C

K

F

I

C

G

V

D

G

G

R

K

-

P

-

G

-

E

V

I

T

T

R

R

A

K

I

L

Q

M

Q

E

W

E

Y

F

A

S

active site: F32 (= F26), G34 (≠ T28), K150 (= K140), E183 (= E173), L206 (≠ I197)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R45), G100 (= G91), L101 (≠ Y92), K150 (= K140), Y154 (≠ R144), E183 (= E173), G186 (≠ M176), D187 (≠ S177), L206 (≠ I197), I209 (= I200), T210 (≠ L201), G245 (≠ N236), T246 (≠ S237)

4cmfA The (r)-selective transaminase from nectria haematococca with inhibitor bound (see paper)
28% identity, 92% coverage: 16:269/275 of query aligns to 48:309/322 of 4cmfA

query
sites
4cmfA

D

D

R

Q

G

G

F

F

Q

M

Y

H

G

S

D

D

G

L

L

T

F
x
Y

E

D

T
x
V

I

P

A

S

V

V

C

W

N

D

G

G

R

R

P

F

L

F

F

R

L

L

Q

E

R

D

H

H

L

L

D

N

R
|
R

L

L

L

E

K

A

G

S

C

C

R

K

-

K

-

M

R

R

L

L

H

R

I

M

P

P

P

L

P

P

D

R

L

E

S

E

L

V

L

I

E

K

S

T

E

L

A

V

R

D

Q

M

L

V

S

A

-

K

A

S

G

G

A

I

D

R

V

D

A

A

V
x
F

L

V

K

E

L

L

I

I

L

V

T

T

R

R

G

G

S

L

G

T

G

G

R

V

G

R

Y

G

R

A

Q

K

P

P

E

E

Q

E

I

L

V

L

P

N

T

N

R

N

L

L

F

Y

S

M

L

F

H

I

P

Q

S

P

P

Y

N

V

Y

W

-

V

-

M

-

D

P

P

D

D

C

V

Y

Q

Q

Y

T

T

D

G

G

G

-

R

-

A

I

I

V

V

A

A

R

R

F

T

C

V

E

R

W

-

R

-

L

-

A

R

I

V

N

P

P

P

G

G

F

S

-

I

-

D

A

P

G

T

I

I

K
|
K

H

N

M

L

N

Q

R

W

L

G

E

D

Q

L

V

V

M

R

A

G

R

L

A

F

E

E

W

A

Q

N

H

D

D

R

T

G

V

A

Q

T

E

Y

G

P

L

F

M

L

F

T

D

D

S

G

D

D

D

A

R

N

I

L

V

T

E
|
E

G

G

T

S

M
x
G

S
x
F

N
|
N

L

V

F

V

F

L

V

I

K

K

D

D

G

G

I

V

L

L

H

Y

T

T

P

P

L

-

V

-

N

D

C

R

G

G

I

V

-
x
L

A

Q

G
|
G

I
|
I

L
x
T

R

R

Q

K

I

S

V

V

I

I

E

D

F

A

A

A

V

R

R

S

N

C

G

G

L

Y

A

E

L

I

S

R

E

V

G

E

H

H

F

V

S

P

K

I

Q

E

N

A

V

T

L

Y

E

Q

A

A

D

D

E

E

V

I

F

L

V

M

T

C

N
x
T

S
x
T

V
x
A

I

G

G

G

I

I

W

M

P

P

V

I

K

T

Q

T

L

L

E

D

K

D

Q

K

C

P

F

V

-

K

-

D

-

G

C

K

V

V

G

G

R

P

V

I

T

T

R

K

A

A

I

I

Q

W

Q

D

W

R

Y

Y

A

W

G

A

A

M

H

H

active site: Y58 (≠ F26), V60 (≠ T28), K179 (= K140), E212 (= E173), L234 (vs. gap)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: V60 (≠ T28), R77 (= R45), F113 (≠ V78), K179 (= K140), E212 (= E173), G215 (≠ M176), F216 (≠ S177), N217 (= N178), L234 (vs. gap), G236 (= G199), I237 (= I200), T238 (≠ L201), T273 (≠ N236), T274 (≠ S237), A275 (≠ V238)

4cmdA The (r)-selective transaminase from nectria haematococca (see paper)
28% identity, 92% coverage: 16:269/275 of query aligns to 48:309/322 of 4cmdA

query
sites
4cmdA

D

D

R

Q

G

G

F

F

Q

M

Y

H

G

S

D

D

G

L

L

T

F
x
Y

E

D

T
x
V

I

P

A

S

V

V

C

W

N

D

G

G

R

R

P

F

L

F

F

R

L

L

Q

E

R

D

H

H

L

L

D

N

R
|
R

L

L

L

E

K

A

G

S

C

C

R

K

-

K

-

M

R

R

L

L

H

R

I

M

P

P

P

L

P

P

D

R

L

E

S

E

L

V

L

I

E

K

S

T

E

L

A

V

R

D

Q

M

L

V

S

A

-

K

A

S

G

G

A

I

D

R

V

D

A

A

V

F

L

V

K

E

L

L

I

I

L

V

T

T

R

R

G

G

S

L

G

T

G

G

R

V

G

R

Y

G

R

A

Q

K

P

P

E

E

Q

E

I

L

V

L

P

N

T

N

R

N

L

L

F

Y

S

M

L

F

H

I

P

Q

S

P

P

Y

N

V

Y

W

-

V

-

M

-

D

P

P

D

D

C

V

Y

Q

Q

Y

T

T

D

G

G

G

-

R

-

A

I

I

V

V

A

A

R

R

F

T

C

V

E

R

W

-

R

-

L

-

A

R

I

V

N

P

P

P

G

G

F

S

-

I

-

D

A

P

G

T

I

I

K
|
K

H

N

M

L

N

Q

R

W

L

G

E

D

Q

L

V

V

M

R

A

G

R

L

A

F

E

E

W

A

Q

N

H

D

D

R

T

G

V

A

Q

T

E

Y

G

P

L

F

M

L

F

T

D

D

S

G

D

D

D

A

R

N

I

L

V

T

E
|
E

G

G

T

S

M
x
G

S
x
F

N

N

L

V

F

V

F

L

V

I

K

K

D

D

G

G

I

V

L

L

H

Y

T

T

P

P

L

-

V

-

N

D

C

R

G

G

I

V

-
x
L

A

Q

G
|
G

I
|
I

L
x
T

R

R

Q

K

I

S

V

V

I

I

E

D

F

A

A

A

V

R

R

S

N

C

G

G

L

Y

A

E

L

I

S

R

E

V

G

E

H

H

F

V

S

P

K

I

Q

E

N

A

V

T

L

Y

E

Q

A

A

D

D

E

E

V

I

F

L

V

M

T

C

N

T

S
x
T

V

A

I

G

G

G

I

I

W

M

P

P

V

I

K

T

Q

T

L

L

E

D

K

D

Q

K

C

P

F

V

-

K

-

D

-

G

C

K

V

V

G

G

R

P

V

I

T

T

R

K

A

A

I

I

Q

W

Q

D

W

R

Y

Y

A

W

G

A

A

M

H

H

active site: Y58 (≠ F26), V60 (≠ T28), K179 (= K140), E212 (= E173), L234 (vs. gap)
binding pyridoxal-5'-phosphate: R77 (= R45), K179 (= K140), E212 (= E173), G215 (≠ M176), F216 (≠ S177), L234 (vs. gap), G236 (= G199), I237 (= I200), T238 (≠ L201), T274 (≠ S237)

7p3tB Transaminase of gamma-proteobacterium (see paper)
28% identity, 96% coverage: 1:265/275 of query aligns to 5:279/299 of 7p3tB

query
sites
7p3tB

M

I

F

I

L

Y

L

I

N

N

G

G

E

D

-

Y

-

L

-

P

-

L

S

S

R

Q

H

A

C

R

V

V

D

S

V

P

S

V

D

D

R

Q

G

G

F

F

Q

L

Y

L

G

G

D

D

G

G

L

V

F

F

E

D

T

V

I

V

A

S

V

A

C

W

N

K

G

G

R

N

P

I

L

F

F

K

L

L

Q

D

R

A

H

H

L

L

D

D

R
|
R

L

F

-

F

-

D

-

S

L

I

K

Q

G

A

C

A

R

R

R

L

L

N

H

H

I

D

P

M

P

S

P

R

D

D

L

A

-

W

-

K

-

E

S

A

L

I

E

E

S

T

E

T

A

R

R

R

Q

N

L

-

S

-

A

-

G

G

A

L

D

D

V

D

A

A

V

S

L

I

K

R

L

F

I

I

L

V

T

T

R

R

G

G

S

E

G

P

G

K

R

G

G

V

Y

V

R

A

Q

D

P

P

E

R

Q

D

I

F

V

K

P

P

T

T

R

C

L

I

F

V

S

W

L

V

H

A

P

P

S

Y

P

I

N

F

Y

L

P

A

D

D

C

E

Y

E

Q

K

T

R

-

R

D

N

G

G

I

I

V

R

A

L

R

M

F

I

C

S

E

A

W

T

R

R

-

G

-

F

-

P

-

A

L

D

A

T

I

L

N

D

P

P

G

R

F

Y

A

-

G

-

I

-

K
|
K

H

C

M

L

N

D

R
|
R

L

L

E

H

Q

S

V

Q

M

L

A

I

R

R

A

L

E

E

W

A

Q

L

H

E

D

A

T

G

V

Y

Q

D

E

D

G

A

L

L

M

W

F

L

D

D

S

H

D

S

D

G

R

H

I

V

V

S

E
|
E

G
x
S

T
x
A

M
x
A

S
|
S

N

N

L

L

F

F

F

I

V

V

K

K

D

N

G

G

I

V

L

L

H

Y

T

T

P

P

L

-

V

-

N

S

C

A

G

G

I

I

-

L

A

R

G
|
G

I
|
I

L
x
T

R

R

Q

D

I

T

V

I

I

L

E

E

F

L

A

A

V

T

R

E

N

L

G

D

L

I

A

P

L

W

S

K

E

E

G

R

H

Q

F

L

S

S

K

A

Q

F

N

D

V

V

L

Y

E

I

A

A

D

D

E

E

V

V

F

F

V

T

T

C

N

S

S
x
T

V

A

I

G

G

G

I

A

W

L

P

P

V

V

K

R

Q

E

L

V

E

A

K

G

Q

R

C

T

F

I

-

R

-

G

-

T

C

T

V

P

G

G

R

P

V

I

T

T

R

Q

A

A

I

I

Q

D

Q

N

W

A

Y

Y

binding pyridoxal-5'-phosphate: R53 (= R45), K153 (= K140), R157 (= R144), E186 (= E173), S187 (≠ G174), A188 (≠ T175), A189 (≠ M176), S190 (= S177), G210 (= G199), I211 (= I200), T212 (≠ L201), T248 (≠ S237)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
26% identity, 95% coverage: 4:265/275 of query aligns to 15:282/301 of 6thqB

query
sites
6thqB

L

L

N

V

G

D

E

E

S

E

R

E

H

A

C

K

V

V

D

T

V

V

S

L

D

S

R

P

G

S

F

L

Q

N

Y

Y

G

G

D

F

G

G

L

V

F
|
F

E

E

T

G

I

I

-

R

A

A

V

Y

C

W

N

N

G

G

R

E

P

N

L

L

F

Y

L

V

Q

F

R

R

-

L

-

R

-

D

H

H

L

M

D

E

R
|
R

L

L

K

R

G

S

C

A

R

K

R

I

L

I

H

G

I

L

P

D

P

V

P

P

D

-

L

-

S

-

L

-

E

-

S

Y

E

T

A

A

R

E

Q

E

L

L

S

S

A

K

G

-

A

-

D

-

V

-

A

A

V

V

L

V

K

E

L

T

I

V

L

R

T

A

R

N

G

G

S

F

G

K

G

E

R

D

G

L

Y
|
Y

R

I

Q

R

P

P

E

V

Q

A

I

Y

V

I

P

S

T

K

R

P

L

Q

F

I

S

S

L

L

H

D

-

V

-

R

-

G

-

L

-

Q

-

A

-

S

-

V

-

A

-

I

P

A

S

A

P

I

N

P

Y

F

P

G

D

K

C

Y

Y

L

Q

K

T

V

D

E

G

G

I

V

V

R

A

A

R

A

F

V

C

V

E

S

W

W

R

R

-

R

-

V

-

H

L

T

A

S

I

M

N

M

P

P

G

V

F

M

A

A

G
x
K

I

A

K

T

H

G

M

I

N
x
Y

R

-

L

L

E

N

Q

S

V

I

M

M

A

A

R

A

A

V

E

E

W

A

Q

R

H

A

D

R

T

G

V

Y

Q

D

E

E

G

A

L

I

M

M

F

L

D

N

S

A

D

E

D

G

R

K

I

V

V

V

E
|
E

G

G

T

S

M
x
G

S
x
E

N
|
N

L

I

F

F

F

I

V

V

K

R

D

R

G

G

I

V

L

L

H

M

T

T

P

P

L

P

L

L

V

E

N

D

C

G

G

I

I
x
L

A

E

G
|
G

I
|
I

L
x
T

R

R

Q

E

I

T

V

V

I

I

E

S

F

I

A

A

V

G

R

D

N

L

G

G

L

I

A

P

L

L

S

L

E

E

G

K

H

S

F

I

S

T

K

R

Q

E

N

E

V

L

L

Y

E

A

A

A

D

D

E

E

V

A

F

F

V

F

T

V

N
x
G

S
x
T

V
x
A

I

A

G

E

I

I

W

T

P

P

V

I

K

I

Q

E

L

I

E

D

K

G

Q

R

C

V

F

L

C

Q

V

R

G

G

R

P

V

I

T

T

R

Q

A

K

I

I

Q

A

Q

E

W

T

Y

Y

active site: F37 (= F26), K156 (≠ G138), E190 (= E173), L214 (≠ I197)
binding pyridoxal-5'-phosphate: R60 (= R45), K156 (≠ G138), Y161 (≠ N143), E190 (= E173), N195 (= N178), L214 (≠ I197), G216 (= G199), I217 (= I200), T218 (≠ L201), T254 (≠ S237)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R45), Y97 (= Y92), K156 (≠ G138), Y161 (≠ N143), E190 (= E173), G193 (≠ M176), E194 (≠ S177), N195 (= N178), G216 (= G199), I217 (= I200), T218 (≠ L201), G253 (≠ N236), T254 (≠ S237), A255 (≠ V238)

6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
26% identity, 92% coverage: 16:269/275 of query aligns to 48:309/321 of 6fteB

query
sites
6fteB

D

D

R

Q

G

G

F

F

Q

L

Y

H

G

S

D

D

G

L

L

T

F
x
Y

E

D

T

V

I

P

A

S

V

V

C

W

N

D

G

G

R

R

P

F

L

F

F

R

L

L

Q

D

R

D

H

H

L

L

D

D

R
|
R

L

F

L

E

K

L

G

S

C

C

R

S

R

K

L

M

H

R

I

F

P

K

P

M

P

P

D

-

L

-

S

-

L

L

L

P

E

R

S

Q

E

E

A

V

R

K

Q

R

L

I

-

L

-

V

-

D

-

M

-

V

-

A

S

K

A

S

G

G

A

I

D

K

V

D

A

A

V

F

L

V

K

E

L

I

I

I

L

V

T

T

R

R

G

G

-

L

S

K

G

G

G

V

R

R

G

G

Y

L

R

K

Q

A

P

G

E

E

Q

S

I

L

V

T

P

N

T

N

R

L

L

Y

F

M

S

W

L

I

H

Q

P

P

S

Y

-

I

-

W

P

V

N

M

Y

E

P

P

D

E

C

M

Y

Q

Q

R

T

T

D

G

G

G

-

S

-

A

I

I

V

I

A

A

R

R

F

T

C

V

E

K

W

-

R

R

L

T

A

S

I

P

N

G

P

S

G

M

F

D

A

P

G

T

I

V

K
|
K

H

N

M

L

N

Q

R

W

L

G

E

D

Q

L

V

T

M

R

A

G

R

M

A

L

E

E

W

A

Q

Q

H

D

D

R

T

G

V

A

Q

D

E

Y

G

P

L

F

M

L

F

T

D

D

S

G

D

D

D

G

R

N

I

I

V

T

E
|
E

G

G

T

S

M

G

S
x
F

N
|
N

L

I

F

V

F

F

V

I

K

K

D

D

G

G

I

V

L

L

H

Y

T

T

P

P

L

-

V

-

N

D

C

R

G

G

I

V

-
x
L

A

K

G
|
G

I
x
V

L
x
T

R

R

Q

K

I

S

V

V

I

A

E

D

F

A

A

A

V

K

R

A

N

N

G

G

L

I

A

E

L

M

S

R

E

I

G

E

H

F

F

V

S

P

K

T

Q

E

N

M

V

A

L

Y

E

Q

A

C

D

D

E

E

V

C

F

F

V

M

T

C

N

T

S
x
T

V

A

I

G

G

G

I

V

W

M

P

P

V

I

K

T

Q

S

L

M

E

D

K

G

Q

Q

C

P

F

I

C

G

-

D

-

G

-

K

V

V

G

G

R

P

V

V

T

T

R

K

A

E

I

I

Q

W

Q

D

W

G

Y

Y

A

W

G

A

A

M

H

H

active site: Y58 (≠ F26), K179 (= K140), E212 (= E173), L234 (vs. gap)
binding pyridoxal-5'-phosphate: R77 (= R45), K179 (= K140), E212 (= E173), F216 (≠ S177), N217 (= N178), L234 (vs. gap), G236 (= G199), V237 (≠ I200), T238 (≠ L201), T274 (≠ S237)

6xwbA Crystal structure of an r-selective transaminase from thermomyces stellatus. (see paper)
28% identity, 92% coverage: 16:269/275 of query aligns to 48:308/319 of 6xwbA

query
sites
6xwbA

D

D

R

E

G

G

F

F

Q

M

Y

R

G

S

D

D

G

L

L

T

F

Y

E

D

T

V

I

P

A

S

V

V

C

W

N

D

G

G

R

R

P

F

L

F

F

R

L

L

Q

D

R

D

H

H

L

L

D

S

R
|
R

L

L

L

E

K

A

G

S

C

C

R

A

R

K

L

L

H

R

I

L

P

-

D

K

L

L

S

P

L

L

L

P

E

R

S

E

E

E

A

V

R

K

Q

K

L

I

-

L

-

V

-

E

-

M

-

V

-

A

S

K

A

S

G

G

A

I

D

R

V

D

A

A

V

F

L

V

K

E

L

I

I

I

L

V

T

T

R

R

G

G

-

L

S

K

G

G

G

V

R

R

G

G

Y

S

R

R

Q

-

P

P

E

E

Q

E

I

I

V

V

P

-

T

N

R

R

L

L

F

Y

S

M

L

L

H

V

P

Q

S

P

P

Y

N

V

Y

W

-

V

-

M

-

E

P

P

D

E

C

V

Y

Q

Q

P

T

V

-

G

D

D

G

A

I

V

V

I

A

A

R

R

F

T

C

V

E

R

W

-

R

-

L

-

A

R

I

V

N

P

P

P

G

G

F

S

-

I

-

D

A

P

G

T

I

V

K
|
K

H

N

M

L

N

Q

R

W

L

G

E

D

Q
x
F

V

V

M

R

A

G

R

L

A

F

E

E

W

A

Q

S

H

D

D

R

T

G

V

A

Q

T

E

Y

G

P

L

F

M

L

F

T

D

D

S

G

D

D

D

A

R

N

I

L

V

T

E
|
E

G

G

T

S

M
x
G

S
x
F

N
|
N

L

I

F

V

F

L

V

V

K

K

D

D

G

G

I

V

L

L

H

Y

T

T

P

P

L

-

V

-

N

D

C

R

G

G

I

V

-
x
L

A

Q

G
|
G

I
x
V

L
x
T

R

R

Q

K

I

S

V

V

I

I

E

D

F

V

A

A

V

N

R

A

N

K

G

G

L

F

A

E

L

V

S

R

E

V

G

E

H

Y

F

V

S

P

K

V

Q

E

N

A

V

A

L

Y

E

H

A

A

D

D

E

E

V

I

F

F

V

M

T

C

N

T

S
x
T

V

A

I

G

G

G

I

I

W

M

P

P

V

I

K

R

Q

S

L

L

E

D

K

G

Q

K

C

P

F

V

C

N

-

D

-

G

-

K

V

V

G

G

R

P

V

I

T

T

R

K

A

A

I

I

Q

W

Q

D

W

G

Y

Y

A

W

G

E

A

M

H

H

binding pyridoxal-5'-phosphate: R77 (= R45), K178 (= K140), F185 (≠ Q147), E211 (= E173), G214 (≠ M176), F215 (≠ S177), N216 (= N178), L233 (vs. gap), G235 (= G199), V236 (≠ I200), T237 (≠ L201), T273 (≠ S237)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
28% identity, 93% coverage: 12:267/275 of query aligns to 19:287/304 of 1iyeA

query
sites
1iyeA

V

V

D

H

V

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

I

I

A

R

V

C

C

Y

N

D

-

S

-

H

-

K

G

G

R

P

P

V

L

V

F

F

L

R

Q

H

R

R

-

E

H

H

L

M

D

Q

R
|
R

L

L

L

H

K

D

G

S

C

A

R

K

R

I

L

Y

H

R

I

F

P

P

P

-

D

-

L

-

S

-

L

V

L

S

E

Q

S

S

E

I

A

D

R

E

Q

L

L

M

S

E

A

A

G

C

A

R

D

D

V

V

-

I

-

R

-

K

-

N

-

L

-

T

-

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

L

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

Q

P

P

P

E

A

Q

G

I

Y

V

S

P

T

T

D

R

V

L

I

F

I

S

A

L

A

H

F

P

P

S

W

P

G

N

A

Y
|
Y

P

L

D

G

C

A

Y

E

Q

A

T

L

D

E

-

Q

G

G

I

I

V

D

A

A

R

M

F

V

C

S

E

S

W

W

-

N

R

R

L

A

A

A

I

P

N

N

P

T

-

I

G

P

F

T

A

A

G

A

I
x
K

K
x
A

H

G

M

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

M

L

A

V

R

G

A

S

E

E

W

A

Q

R

H

R

D

H

T

G

V

Y

Q

Q

E

E

G

G

L

I

M

A

F

L

D

D

S

V

D

N

D

G

R

Y

I

I

V

S

E
|
E

G

G

T

A

M
x
G

S
x
E

N
|
N

L

L

F

F

F

E

V

V

K

K

D

D

G

G

I

V

L

L

H

F

T

T

P

P

L

P

L

F

V

T

N

S

C

S

G

A

I
x
L

A

P

G
|
G

I
|
I

L
x
T

R

R

Q

D

I

A

V

I

I

I

E

K

F

L

A

A

V

K

R

E

N

L

G

G

L

I

A

E

L

V

S

R

E

E

G

Q

H

V

F

L

S

S

K

R

Q

E

N

S

V

L

L

Y

E

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

N
x
G

S
x
T

V
x
A

I

A

G

E

I

I

W

T

P

P

V

V

K

R

Q

S

L

V

E

D

K

G

Q

I

C

Q

F

V

C

G

V

E

G

G

R

R

-

C

-

G

-

P

V

V

T

T

R

K

A

R

I

I

Q

Q

W

A

Y

F

A

F

G

G

active site: F33 (= F26), G35 (≠ T28), K156 (≠ I139), A157 (≠ K140), E190 (= E173), L214 (≠ I197)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R45), Y92 (≠ V78), Y126 (= Y112), K156 (≠ I139), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), E194 (≠ S177), N195 (= N178), L214 (≠ I197), G216 (= G199), I217 (= I200), T218 (≠ L201), G253 (≠ N236), T254 (≠ S237), A255 (≠ V238)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
28% identity, 93% coverage: 12:267/275 of query aligns to 19:287/304 of 1iydA

query
sites
1iydA

V

V

D

H

V

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

I

I

A

R

V

C

C

Y

N

D

-

S

-

H

-

K

G

G

R

P

P

V

L

V

F

F

L

R

Q

H

R

R

-

E

H

H

L

M

D

Q

R
|
R

L

L

L

H

K

D

G

S

C

A

R

K

R

I

L

Y

H

R

I

F

P

P

P

-

D

-

L

-

S

-

L

V

L

S

E

Q

S

S

E

I

A

D

R

E

Q

L

L

M

S

E

A

A

G

C

A

R

D

D

V

V

-

I

-

R

-

K

-

N

-

L

-

T

-

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

L

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

Q

P

P

P

E

A

Q

G

I

Y

V

S

P

T

T

D

R

V

L

I

F

I

S

A

L

A

H

F

P

P

S

W

P

G

N

A

Y
|
Y

P

L

D

G

C

A

Y

E

Q

A

T

L

D

E

-

Q

G

G

I

I

V

D

A

A

R

M

F

V

C

S

E

S

W

W

-

N

R

R

L

A

A

A

I

P

N

N

P

T

-

I

G

P

F

T

A

A

G

A

I
x
K

K
x
A

H

G

M

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

M

L

A

V

R

G

A

S

E

E

W

A

Q

R

H

R

D

H

T

G

V

Y

Q

Q

E

E

G

G

L

I

M

A

F

L

D

D

S

V

D

N

D

G

R

Y

I

I

V

S

E
|
E

G

G

T

A

M
x
G

S
x
E

N

N

L

L

F

F

F

E

V

V

K

K

D

D

G

G

I

V

L

L

H

F

T

T

P

P

L

P

L

F

V

T

N

S

C

S

G

A

I
x
L

A

P

G
|
G

I
|
I

L
x
T

R

R

Q

D

I

A

V

I

I

I

E

K

F

L

A

A

V

K

R

E

N

L

G

G

L

I

A

E

L

V

S

R

E

E

G

Q

H

V

F

L

S

S

K

R

Q

E

N

S

V

L

L

Y

E

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

N

G

S
x
T

V
x
A

I

A

G

E

I

I

W

T

P

P

V

V

K

R

Q

S

L

V

E

D

K

G

Q

I

C

Q

F

V

C

G

V

E

G

G

R

R

-

C

-

G

-

P

V

V

T

T

R

K

A

R

I

I

Q

Q

W

A

Y

F

A

F

G

G

active site: F33 (= F26), G35 (≠ T28), K156 (≠ I139), A157 (≠ K140), E190 (= E173), L214 (≠ I197)
binding glutaric acid: Y92 (≠ V78), Y126 (= Y112), A255 (≠ V238)
binding pyridoxal-5'-phosphate: R56 (= R45), K156 (≠ I139), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), E194 (≠ S177), L214 (≠ I197), G216 (= G199), I217 (= I200), T218 (≠ L201), T254 (≠ S237)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
28% identity, 93% coverage: 12:267/275 of query aligns to 19:287/304 of 1i1mA

query
sites
1i1mA

V

V

D

H

V

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F

F

E

E

T

G

I

I

A

R

V

C

C

Y

N

D

-

S

-

H

-

K

G

G

R

P

P

V

L

V

F

F

L

R

Q

H

R

R

-

E

H

H

L

M

D

Q

R
|
R

L

L

L

H

K

D

G

S

C

A

R

K

R

I

L

Y

H

R

I

F

P

P

P

-

D

-

L

-

S

-

L

V

L

S

E

Q

S

S

E

I

A

D

R

E

Q

L

L

M

S

E

A

A

G

C

A

R

D

D

V

V

-

I

-

R

-

K

-

N

-

L

-

T

-

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

L

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

Q

P

P

P

E

A

Q

G

I

Y

V

S

P

T

T

D

R

V

L

I

F

I

S

A

L

A

H

F

P

P

S

W

P

G

N

A

Y

Y

P

L

D

G

C

A

Y

E

Q

A

T

L

D

E

-

Q

G

G

I

I

V

D

A

A

R

M

F

V

C

S

E

S

W

W

-

N

R

R

L

A

A

A

I

P

N

N

P

T

-

I

G

P

F

T

A

A

G

A

I
x
K

K

A

H

G

M

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

M

L

A

V

R

G

A

S

E

E

W

A

Q

R

H

R

D

H

T

G

V

Y

Q

Q

E

E

G

G

L

I

M

A

F

L

D

D

S

V

D

N

D

G

R

Y

I

I

V

S

E
|
E

G

G

T

A

M
x
G

S
x
E

N

N

L

L

F

F

F

E

V

V

K

K

D

D

G

G

I

V

L

L

H

F

T

T

P

P

L

P

L

F

V

T

N

S

C

S

G

A

I
x
L

A

P

G
|
G

I
|
I

L
x
T

R

R

Q

D

I

A

V

I

I

I

E

K

F

L

A

A

V

K

R

E

N

L

G

G

L

I

A

E

L

V

S

R

E

E

G

Q

H

V

F

L

S

S

K

R

Q

E

N

S

V

L

L

Y

E

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

N
x
G

S
x
T

V
x
A

I

A

G

E

I

I

W

T

P

P

V

V

K

R

Q

S

L

V

E

D

K

G

Q

I

C

Q

F

V

C

G

V

E

G

G

R

R

-

C

-

G

-

P

V

V

T

T

R

K

A

R

I

I

Q

Q

W

A

Y

F

A

F

G

G

active site: K156 (≠ I139)
binding 4-methyl valeric acid: Y92 (≠ V78), K156 (≠ I139), T254 (≠ S237), A255 (≠ V238)
binding pyridoxal-5'-phosphate: R56 (= R45), K156 (≠ I139), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), E194 (≠ S177), L214 (≠ I197), G216 (= G199), I217 (= I200), T218 (≠ L201), G253 (≠ N236), T254 (≠ S237)

Query Sequence

>WP_013820081.1 NCBI__GCF_000214665.1:WP_013820081.1
MFLLNGESRHCVDVSDRGFQYGDGLFETIAVCNGRPLFLQRHLDRLLKGCRRLHIPPPDL
SLLESEARQLSAGADVAVLKLILTRGSGGRGYRQPEQIVPTRLFSLHPSPNYPDCYQTDG
IVARFCEWRLAINPGFAGIKHMNRLEQVMARAEWQHDTVQEGLMFDSDDRIVEGTMSNLF
FVKDGILHTPLLVNCGIAGILRQIVIEFAVRNGLALSEGHFSKQNVLEADEVFVTNSVIG
IWPVKQLEKQCFCVGRVTRAIQQWYAGAHKLEANL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory