SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013820404.1 NCBI__GCF_000214665.1:WP_013820404.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
68% identity, 98% coverage: 6:317/317 of query aligns to 7:317/318 of Q63XL8

query
sites
Q63XL8

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RQ 99:100 (= RQ 98:99) binding ATP

6asvC E. Coli prpp synthetase (see paper)
66% identity, 97% coverage: 8:315/317 of query aligns to 4:311/311 of 6asvC

query
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binding phosphate ion: D168 (= D172), D218 (= D222), D219 (= D223), D222 (= D226), T223 (= T227), G224 (≠ A228), T226 (= T230), L227 (= L231)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
66% identity, 97% coverage: 8:315/317 of query aligns to 6:313/315 of P0A717

query
sites
P0A717

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D129 (= D131) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D222) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D223) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D226) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
66% identity, 96% coverage: 8:311/317 of query aligns to 5:308/308 of 4s2uA

query
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4s2uA

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binding magnesium ion: D223 (= D226), T227 (= T230)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
64% identity, 97% coverage: 8:315/317 of query aligns to 4:305/307 of 7xmvA

query
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7xmvA

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K

R

L

L

N

Y

M

T

R

S

L

L

G

G

M

D

A

A

S

A

V

V

D

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

F

S

V

V

E

Q

I

I

E

N

E

E

N

N

V

V

R

R

G

G

R

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

C

C

S

A

P

P

T

T

N

N

E

D

N

N

L

L

M

M

E

E

L

L

L

V

V

V

M

M

I

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

R

R

S
x
V

R
|
R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

V

V

A

A

D

D

M

F

I

L

G

S

H

S

A

V

G

G

A

V

D

D

R

R

A

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

V

P

P

V

V

D

D

N

N

V

V

Y
x
F

A

G

S
|
S

P

P

I

I

L

L

G

E

D

D

V

M

W

L

R

Q

Q

L

E

N

Y

L

E

D

N

N

L

P

I

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A
|
A

L

I

A

A

K

K

R

L

L

L

G

N

D

D

A

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

V

-

S

-

E

Q

I

V

M

M

H

H

I

I

G

G

D

D

V

V

D

A

G

G

R

R

T

D

C

C

V

V

M

L

V

V

D
|
D

L
x
M

V

I

D
|
D

T
|
T

A

G

G
|
G

T
|
T

L

L

C

C

H

K

A

A

S

E

A

A

L

L

K

K

K

E

N

R

G

G

A

A

V

K

K

R

V

V

V

F

A

A

Y

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

A

N

A

A

D

N

N

N

V

L

R

R

N

N

S

S

V

V

L

I

D

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

T

S

D

D

E

E

L

I

K

K

A

S

I

L

G

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

E

G

M

M

L

L

A

A

E

E

T

A

I

I

R

R

I

I

A

S

V

N

G

E

E

E

S

S

V

I

S

S

S

A

L

M

Y

F

binding adenosine-5'-diphosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R98), R97 (= R101), H129 (= H133)
binding adenosine monophosphate: R97 (= R101), V99 (≠ S103), R100 (= R104), E131 (≠ D135), F145 (≠ Y149), S147 (= S151), V173 (= V177), A177 (= A181)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D222), D213 (= D223), M214 (≠ L224), D216 (= D226), T217 (= T227), G219 (= G229), T220 (= T230)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
64% identity, 97% coverage: 8:315/317 of query aligns to 4:305/307 of 7xmuA

query
sites
7xmuA

V

L

F

F

S

A

G

G

N

N

A

A

N

T

K

P

A

E

L

L

S

A

E

Q

G

R

I

I

V

A

R

N

K

R

L

L

N

Y

M

T

R

S

L

L

G

G

M

D

A

A

S

A

V

V

D

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

F

S

V

V

E

Q

I

I

E

N

E

E

N

N

V

V

R

R

G

G

R

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

C

C

S

A

P

P

T

T

N

N

E

D

N

N

L

L

M

M

E

E

L

L

L

V

V

V

M

M

I

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

S

V

R
|
R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

V

V

A

A

D

D

M

F

I

L

G

S

H

S

A

V

G

G

A

V

D

D

R

R

A

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

V

P

P

V

V

D

D

N

N

V

V

Y
x
F

A

G

S
|
S

P

P

I

I

L

L

G

E

D

D

V

M

W

L

R

Q

Q

L

E

N

Y

L

E

D

N

N

L

P

I

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A

A

L

I

A

A

K

K

R

L

L

L

G

N

D

D

A

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

V

-

S

-

E

Q

I

V

M

M

H

H

I

I

G

G

D

D

V

V

D

A

G

G

R

R

T

D

C

C

V

V

M

L

V

V

D
|
D

D

D

L
x
M

V

I

D
|
D

T
|
T

A

G

G

G

T

T

L

L

C

C

H

K

A

A

S

E

A

A

L

L

K

K

K

E

N

R

G

G

A

A

V

K

K

R

V

V

V

F

A

A

Y

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

A

N

A

A

D

N

N

N

V

L

R

R

N

N

S

S

V

V

L

I

D

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

T

S

D

D

E

E

L

I

K

K

A

S

I

L

G

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

E

G

M

M

L

L

A

A

E

E

T

A

I

I

R

R

I

I

A

S

V

N

G

E

E

E

S

S

V

I

S

S

S

A

L

M

Y

F

binding adenosine-5'-diphosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R98), Q95 (= Q99), R97 (= R101), R97 (= R101), R100 (= R104), H129 (= H133), E131 (≠ D135), F145 (≠ Y149), S147 (= S151), V173 (= V177)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D172), D212 (= D222), M214 (≠ L224), D216 (= D226), T217 (= T227)

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
67% identity, 97% coverage: 6:311/317 of query aligns to 2:299/300 of 3dahC

query
sites
3dahC

I

L

M

M

V

V

F

F

S

T

G

G

N

N

A

A

N

N

K

P

A

A

L

L

S

A

E

Q

G

E

I

V

V

V

R

K

K

I

L

L

N

G

M

I

R

P

L

L

G

G

M

K

A

A

S

M

V

V

D

S

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

I

F

Q

V

V

E

E

I

I

E

Q

E

E

N

N

V

V

R

R

G

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

C

C

S

A

P

P

T

T

N

N

E

D

N

N

L

L

M

M

E

E

L

L

L

M

V

I

M

M

I

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

A

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

S

P

R

R

S

S

A

A

R

R

V

V

P

A

I

I

T

S

A

A

R

K

L

V

V

V

A

A

D

N

M

M

I

L

G

E

H

I

A

A

G

G

A

V

D

E

R

R

A

I

L

I

T

T

V

M

D

D

L

L

H

H

A

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

V

D

D

N

N

V

I

Y

Y

A

A

S

T

P

P

I

I

L

L

G

G

D

D

V

L

W

R

R

K

Q

Q

E

N

Y

Y

E

P

N

D

L

L

I

L

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

A

A

L

L

A

A

K

K

R

Q

L

L

G

-

D

N

A

C

D

D

L

L

A

A

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

V

-

S

-

E

-

I

V

M

M

H

N

I

I

G

G

D

E

V

V

D

E

G

G

R

R

T

T

C

C

V

V

M

I

V

M

D

D

L

M

V

V

D

D

T

T

A

A

G

G

T

T

L

L

C

C

H

K

A

A

S

Q

A

V

L

L

K

K

K

E

N

R

G

G

A

A

V

K

K

Q

V

V

V

F

A

A

Y

Y

C

A

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

G

A

A

D

D

N

R

V

I

R

A

N

A

S

S

V

A

L

L

D

D

E

E

L

L

V

V

T

T

D

D

T

T

I

I

P

P

L

L

T

S

D

A

E

E

L

S

K

L

A

A

I

C

G

P

K

K

I

I

R

R

Q

A

L

L

S

S

V

S

A

A

E

G

M

L

L

L

A

A

E

E

T

T

I

F

R

S

R

R

I

I

A

R

V

R

G

G

E

D

S

S

V

V

binding adenosine monophosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R98), Q95 (= Q99)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
63% identity, 96% coverage: 4:307/317 of query aligns to 1:299/299 of 6nfeB

query
sites
6nfeB

A

S

S

T

I

M

M

M

V

I

F

F

S

T

G

G

N

N

A

A

N

N

K

P

A

E

L

L

S

A

E

L

G

K

I

I

V

S

R

S

K

H

L

L

N

Q

M

I

R

P

L

I

G

G

M

K

A

A

S

T

V

V

D

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

F

M

V

V

E

E

I

I

E

L

E

E

N

N

V

V

R

R

G

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

C

C

S

A

P

P

T

A

N

N

E

N

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

A

D

D

A

A

L

L

R

R

A

S

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

I

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

S

V

R

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

V

V

A

A

D

D

M

M

I

M

G

A

H

S

A

V

G

G

A

I

D

C

R

R

A

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

G

Y

I

M

P

P

V

V

D

D

N

N

V

V

Y

Y

A

S

S

T

P

P

I

V

L

L

G

E

D

D

V

I

W

T

R

K

Q

Q

E

K

Y

L

E

N

N

N

L

I

I

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

A

A

L

V

A

A

K

K

R

R

L

L

G

N

D

D

A

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

V

-

S

S

E

E

I

V

M

M

H

H

I

I

G

G

D

E

V

P

D

A

G

N

R

K

T

N

C

C

V

I

M

I

V

V

D
|
D

L
x
I

V

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

C

C

H

T

A

A

S

H

A

E

L

L

K

K

N

N

G

G

A

A

V

K

K

S

V

V

V

R

A

A

Y

Y

C

I

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

P

A

A

A

V

D

N

N

N

V

I

R

K

N

H

S

S

V

G

L

L

D

D

E

E

L

V

V

V

T

T

D

D

T

T

I

I

P

P

L

L

T

S

D

A

E

E

L

A

K

Q

A

N

I

C

G

E

K

K

I

I

R

R

Q

V

L

V

S

S

V

L

A

A

E

D

M

M

L

L

A

A

E

Q

T

A

I

I

R

K

R

R

I

V

A

N

V

V

binding adenosine-5'-diphosphate: F34 (= F37), D36 (= D39), E38 (= E41), R95 (= R98), Q96 (= Q99), H130 (= H133)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H133), D214 (= D222), D215 (= D223), I216 (≠ L224), D218 (= D226), T219 (= T227), A220 (= A228), T222 (= T230)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
63% identity, 96% coverage: 4:307/317 of query aligns to 1:298/298 of 6nfeA

query
sites
6nfeA

A

S

S

T

I

M

M

M

V

I

F

F

S

T

G

G

N

N

A

A

N

N

K

P

A

E

L

L

S

A

E

L

G

K

I

I

V

S

R

S

K

H

L

L

N

Q

M

I

R

P

L

I

G

G

M

K

A

A

S

T

V

V

D

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

F

M

V

V

E

E

I

I

E

L

E

E

N

N

V

V

R

R

G

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

C

C

S

A

P

P

T

A

N

N

E

N

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

A

D

D

A

A

L

L

R

R

A

S

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

I

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

S
x
V

R

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

V

V

A

A

D

D

M

M

I

M

G

A

H

S

A

V

G

G

A

I

D

C

R

R

A

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

G

Y

I

M

P

P

V

V

D

D

N

N

V

V

Y
|
Y

A

S

S

T

P

P

I

V

L

L

G

E

D

D

V

I

W

T

R

K

Q

Q

E

K

Y

L

E

N

N

N

L

I

I

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R
|
R

A

A

R

R

A
|
A

L

V

A

A

K
|
K

R

R

L

L

G

N

D

D

A

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

V

-

S

-

E

E

I

V

M

M

H

H

I

I

G

G

D

E

V

P

D

A

G

N

R

K

T

N

C

C

V

I

M

I

V

V

D
|
D

L
x
I

V

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

C

C

H

T

A

A

S

H

A

E

L

L

K

K

N

N

G

G

A

A

V

K

K

S

V

V

V

R

A

A

Y

Y

C

I

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

P

A

A

A

V

D

N

N

N

V

I

R

K

N

H

S

S

V

G

L

L

D

D

E

E

L

V

V

V

T

T

D

D

T

T

I

I

P

P

L

L

T

S

D

A

E

E

L

A

K

Q

A

N

I

C

G

E

K

K

I

I

R

R

Q

V

L

V

S

S

V

L

A

A

E

D

M

M

L

L

A

A

E

Q

T

A

I

I

R

K

R

R

I

V

A

N

V

V

binding adenosine-5'-diphosphate: F34 (= F37), D36 (= D39), E38 (= E41), R95 (= R98), Q96 (= Q99), H130 (= H133)
binding adenosine monophosphate: R98 (= R101), V100 (≠ S103), Y146 (= Y149), R175 (= R178), A178 (= A181), K181 (= K184)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H133), D213 (= D222), D214 (= D223), I215 (≠ L224), D217 (= D226), T218 (= T227), A219 (= A228), T221 (= T230)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
53% identity, 99% coverage: 3:315/317 of query aligns to 7:316/317 of P14193

query
sites
P14193

D

D

A

K

S

N

I

L

M

K

V

I

F

F

S

S

G

L

N

N

A

S

N

N

K

P

A

E

L

L

S

A

E

K

G

E

I

I

V

A

R

D

K

I

L

V

N

G

M

V

R

Q

L

L

G

G

M

K

A

C

S

S

V

V

D

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

E

N

I

I

E

E

N

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

P

S

T

T

C

S

S

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

V

I

M

M

I

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

R

K

S

A

R

R

S

S

A

-

R

R

V

E

P

P

I

I

T

T

A

A

R

K

L

L

V

F

A

A

D

N

M

L

I

L

G

E

H

T

A

A

G

G

A

A

D

T

R

R

A

V

L

I

T

A

V

L

D

D

L

L

H

H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

I

D

D

N

H

V

L

Y

M

A

G

S

V

P

P

I

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

Q

K

E

N

Y

L

E

E

N

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

G

-

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K
|
K

R

R

R
|
R

P

P

R
|
R

A

P

N
|
N

V

V

S

A

E
|
E

I

V

M

M

H

N

I

I

I

V

G

G

D

N

V

I

D

E

G

G

R

K

T

T

C

A

V

I

M

L

V

I

D

D

L

I

V

I

D
|
D

T
|
T

A
|
A

G
|
G

T
|
T

L

I

C

T

H

L

A

A

S

N

A

A

L

L

K

V

K

E

N

N

G

G

A

A

V

K

K

E

V

V

V

Y

A

A

Y

C

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

P

A

A

A

V

D

E

N

R

V

I

R

N

N

N

S

S

V

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

T

P

D

E

E

E

L

-

K

K

A

K

I

I

G

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

E

P

M

L

L

L

A

A

E

E

T

A

I

I

R

I

R

R

I

V

A

H

V

E

G

Q

E

Q

S

S

V

V

S

S

S

Y

L

L

Y

F

RQ 102:103 (= RQ 98:99) binding ATP
K198 (= K196) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R198) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (= R200) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N202) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E205) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (= DTAGT 226:230) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
50% identity, 99% coverage: 3:315/317 of query aligns to 1:299/299 of 1ibsB

query
sites
1ibsB

D

D

A

K

S

N

I

L

M

K

V

I

F

F

S

S

G

L

N

N

A

S

N

N

K

P

A

E

L

L

S

A

E

K

G

E

I

I

V

A

R

D

K

I

L

V

N

G

M

V

R

Q

L

L

G

G

M

K

A

C

S

S

V

V

D

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

E

N

I

I

E

E

N

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

P

S

T

T

C

S

S

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

V

I

M

M

I

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

S

S

R

R

S

E

A

-

R

-

V

-

P

P

I

I

T

T

A

A

R

K

L

L

V

F

A

A

D

N

M

L

I

L

G

E

H

T

A

A

G

G

A

A

D

T

R

R

A

V

L

I

T

A

V

L

D

D

L

L

H

H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

I

D

D

N

H

V

L

Y

M

A

G

S

V

P

P

I

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

Q

K

E

N

Y

L

E

E

N

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

G

-

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

R

-

A

-

N

-

V

-

S

-

E

-

I

-

M

M

H

N

I

I

I

V

G

G

D

N

V

I

D

E

G

G

R

K

T

T

C

A

V

I

M

L

V

I

D

D

L

I

V

I

D

D

T

T

A

A

G

G

T

T

L

I

C

T

H

L

A

A

S

N

A

A

L

L

K

V

K

E

N

N

G

G

A

A

V

K

K

E

V

V

V

Y

A

A

Y

C

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

P

A

A

A

V

D

E

N

R

V

I

R

N

N

N

S

S

V

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

T

P

D

E

E

E

L

-

K

K

A

K

I

I

G

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

E

P

M

L

L

L

A

A

E

E

T

A

I

I

R

I

R

R

I

V

A

H

V

E

G

Q

E

Q

S

S

V

V

S

S

S

Y

L

L

Y

F

binding methyl phosphonic acid adenosine ester: R96 (= R98), Q97 (= Q99), R99 (= R101)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
50% identity, 98% coverage: 5:315/317 of query aligns to 1:297/297 of 1ibsA

query
sites
1ibsA

S

N

I

L

M

K

V

I

F

F

S

S

G

L

N

N

A

S

N

N

K

P

A

E

L

L

S

A

E

K

G

E

I

I

V

A

R

D

K

I

L

V

N

G

M

V

R

Q

L

L

G

G

M

K

A

C

S

S

V

V

D

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

E

N

I

I

E

E

N

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

P

S

T

T

C

S

S

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

V

I

M

M

I

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

S

S

R

R

S

E

A

-

R

-

V

-

P

P

I

I

T

T

A

A

R

K

L

L

V

F

A

A

D

N

M

L

I

L

G

E

H

T

A

A

G

G

A

A

D

T

R

R

A

V

L

I

T

A

V

L

D

D

L

L

H
|
H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

I

D

D

N

H

V

L

Y

M

A

G

S

V

P

P

I

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

Q

K

E

N

Y

L

E

E

N

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

G

-

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

R

-

A

-

N

-

V

-

S

-

E

-

I

-

M

M

H

N

I

I

I

V

G

G

D

N

V

I

D

E

G

G

R

K

T

T

C

A

V

I

M

L

V

I

D

D

L

I

V

I

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

I

C

T

H

L

A

A

S

N

A

A

L

L

K

V

K

E

N

N

G

G

A

A

V

K

K

E

V

V

V

Y

A

A

Y

C

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

P

A

A

A

V

D

E

N

R

V

I

R

N

N

N

S

S

V

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

T

P

D

E

E

E

L

-

K

K

A

K

I

I

G

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

E

P

M

L

L

L

A

A

E

E

T

A

I

I

R

I

R

R

I

V

A

H

V

E

G

Q

E

Q

S

S

V

V

S

S

S

Y

L

L

Y

F

binding methyl phosphonic acid adenosine ester: R94 (= R98), Q95 (= Q99), R97 (= R101), H126 (= H133)
binding sulfate ion: D209 (= D226), T210 (= T227), A211 (= A228), T213 (= T230)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
50% identity, 98% coverage: 5:315/317 of query aligns to 1:295/295 of 1dkuA

query
sites
1dkuA

S

N

I

L

M

K

V

I

F

F

S

S

G

L

N

N

A

S

N

N

K

P

A

E

L

L

S

A

E

K

G

E

I

I

V

A

R

D

K

I

L

V

N

G

M

V

R

Q

L

L

G

G

M

K

A

C

S

S

V

V

D

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

E

N

I

I

E

E

N

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

P

S

T

T

C

S

S

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

V

I

M

M

I

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R

R

R
x
K

S

A

R

R

S
|
S

A

-

R
|
R

V

E

P

P

I

I

T

T

A

A

R

K

L

L

V

F

A

A

D

N

M

L

I

L

G

E

H

T

A

A

G

G

A

A

D

T

R

R

A

V

L

I

T

A

V

L

D

D

L

L

H
|
H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

I

D
|
D

N

H

V

L

Y

M

A

G

S

V

P

P

I

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

Q

K

E

N

Y

L

E

E

N

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

G

-

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

R

-

A

-

N

-

V

-

S

-

E

-

I

-

M

M

H

N

I

I

I

V

G

G

D

N

V

I

D

E

G

G

R

K

T

T

C

A

V

I

M

L

V

I

D

D

L

I

V

I

D

D

T

T

A

A

G

G

T

T

L

I

C

T

H

L

A

A

S

N

A

A

L

L

K

V

K

E

N

N

G

G

A

A

V

K

K

E

V

V

V

Y

A

A

Y

C

C

C

T

T

H

H

P

P

V

V

L

L

S

S

G

G

A

P

A

A

A

V

D

E

N

R

V

I

R

N

N

N

S

S

V

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

T

K

D

-

E

-

L

-

K

-

A

-

I

I

G

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

E

P

M

L

L

L

A

A

E

E

T

A

I

I

R

I

R

R

I

V

A

H

V

E

G

Q

E

Q

S
|
S

V
|
V

S
|
S

S

Y

L

L

Y
x
F

binding methyl phosphonic acid adenosine ester: R94 (= R98), H128 (= H133)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R102), S101 (= S105), R102 (= R107), D141 (= D146), S290 (= S310), V291 (= V311), S292 (= S312), F295 (≠ Y315)

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
48% identity, 98% coverage: 5:315/317 of query aligns to 2:312/316 of 8dbkB

query
sites
8dbkB

S

N

I

I

M

K

V

I

F

F

S

S

G

G

N

S

A

S

N

H

K

Q

A

D

L

L

S

S

E

Q

G

K

I

I

V

A

R

D

K

R

L

L

N

G

M

L

R

E

L

L

G

G

M

K

A

V

S

V

V

T

D

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

C

V

V

E

E

I

I

E

G

E

E

N
x
S

V

V

R
|
R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

C

C

S

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

A

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

S

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

V

V

A

A

D

N

M

M

I

L

G

S

H

V

A

A

G

G

A

A

D

D

R

H

A

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

V

D

D

N

N

V

L

Y

Y

A

A

S

E

P

P

I

A

L

V

L

L

G

K

D

W

V

I

W

R

R

E

Q

N

-

I

-

S

E

E

Y

W

E

R

N

N

L

C

I

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G
|
G

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

R

R

L

L

G

-

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

P

K

R

K

A

A

N
|
N

V

E

S

V

E

D

I

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

K

G

D

R

R

T

V

C

A

V

I

M

L

V

V

D
|
D

L

M

V

A

D
|
D

T
|
T

A
x
C

G
|
G

T
|
T

L

I

C

C

H

H

A

A

S

D

A

K

L

L

K

L

K

S

N

A

G

G

A

A

V

T

K

R

V

V

V

Y

A

A

Y

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

A

P

A

A

A

I

D

S

N

R

V

I

R

N

N

N

S

A

V

C

L

F

D

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

T

E

D

D

E

K

L

M

K

K

A

H

I

C

G

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

E

M

M

I

L

L

A

A

E

E

T

A

I

I

R

R

I

T

A

H

V

N

G

G

E

E

S

S

V

V

S

S

S

Y

L

L

Y

F

binding adenosine monophosphate: R95 (= R98), Q96 (= Q99), N199 (= N202)
binding adenosine-5'-triphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41)
binding phosphate ion: S46 (≠ N49), R48 (= R51)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H133), D170 (= D172), G172 (= G174), K193 (= K196), R195 (= R198), D219 (= D222), D220 (= D223), D223 (= D226), T224 (= T227), C225 (≠ A228), G226 (= G229), T227 (= T230)

8dbeA Human prps1 with adp; hexamer (see paper)
48% identity, 98% coverage: 5:315/317 of query aligns to 2:312/316 of 8dbeA

query
sites
8dbeA

S

N

I

I

M

K

V

I

F

F

S

S

G

G

N

S

A

S

N

H

K

Q

A

D

L

L

S

S

E

Q

G

K

I

I

V

A

R

D

K

R

L

L

N

G

M

L

R

E

L

L

G

G

M

K

A

V

S

V

V

T

D

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

C

V

V

E

E

I

I

E

G

E

E

N

S

V

V

R

R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

C

C

S

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

A

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

S
x
D

R

K

S
|
S

A

-

R
|
R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

V

V

A

A

D

N

M

M

I

L

G

S

H

V

A

A

G

G

A

A

D

D

R

H

A

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

V

D
|
D

N

N

V

L

Y
|
Y

A

A

S

E

P

P

I

A

L

V

L

L

G

K

D

W

V

I

W

R

R

E

Q

N

-

I

-

S

E

E

Y

W

E

R

N

N

L

C

I

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

R

R

L

L

G

-

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

K

R

K

A

A

N

N

V

E

S

V

E

D

I

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

K

G

D

R

R

T

V

C

A

V

I

M

L

V

V

D
|
D

L

M

V

A

D
|
D

T
|
T

A

C

G
|
G

T
|
T

L

I

C

C

H

H

A

A

S

D

A

K

L

L

K

L

K

S

N

A

G

G

A

A

V

T

K

R

V

V

V

Y

A

A

Y

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

A

P

A

A

A

I

D

S

N

R

V

I

R

N

N

N

S

A

V

C

L

F

D

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

T

E

D

D

E

K

L

M

K

K

A

H

I

C

G

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

E

M

M

I

L

L

A

A

E

E

T

A

I

I

R

R

I

T

A

H

V

N

G

G

E

E

S
|
S

V
|
V

S
|
S

S

Y

L

L

Y
x
F

binding adenosine-5'-diphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), R95 (= R98), Q96 (= Q99), K98 (≠ R101), K99 (≠ R102), D100 (≠ S103), S102 (= S105), R103 (= R107), H129 (= H133), D142 (= D146), Y145 (= Y149), S307 (= S310), V308 (= V311), S309 (= S312), F312 (≠ Y315)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H133), D170 (= D172), D219 (= D222), D220 (= D223), D223 (= D226), T224 (= T227), G226 (= G229), T227 (= T230)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
48% identity, 98% coverage: 5:315/317 of query aligns to 3:313/318 of P60891

query
sites
P60891

S

N

I

I

M

K

V

I

F

F

S

S

G

G

N

S

A

S

N

H

K

Q

A

D

L

L

S
|
S

E

Q

G

K

I

I

V

A

R

D

K

R

L

L

N

G

M

L

R

E

L

L

G

G

M

K

A

V

S

V

V

T

D

K

R

K

F

F

S

S

D

N

G

Q

E

E

I

T

F

C

V

V

E

E

I

I

E

G

E

E

N

S

V

V

R

R

G

G

R

E

D
|
D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

C

C

S

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

A

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

S

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

V

V

A

A

D
x
N

M

M

I

L

G

S

H

V

A

A

G

G

A

A

D

D

R

H

A

I

L

I

T

T

V

M

D

D

L
|
L

H

H

A

A

D
x
S

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V
|
V

D

D

N
|
N

V

L

Y
|
Y

A

A

S

E

P

P

I

A

L

V

L

L

G

K

D

W

V

I

W

R

R

E

Q

N

-

I

-

S

E

E

Y

W

E

R

N

N

L

C

I

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K
x
D

R

R

L

L

G

-

D

N

A

V

D

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

P

K

R

K

A

A

N

N

V

E

S

V

E
x
D

I

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

K

G

D

R

R

T

V

C

A

V

I

M

L

V
|
V

D

D

L

M

V

A

D

D

T

T

A

C

G

G

T

T

L

I

C

C

H
|
H

A

A

S

D

A

K

L

L

K

L

K

S

N

A

G

G

A

A

V

T

K

R

V

V

V

Y

A

A

Y

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

A

P

A

A

A

I

D

S

N

R

V

I

R

N

N

N

S

A

V

C

L

F

D

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

T

E

D

D

E

K

L

M

K

K

A

H

I

C

G

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

E

M

M

I

L

L

A

A

E

E

T

A

I

I

R

R

I

T

A

H

V

N

G

G

E

E

S

S

V

V

S

S

S

Y

L

L

Y

F

S16 (= S18) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D54) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ D117) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L132) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ D135) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V145) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N147) mutation to H: No effect on catalytic activity.
Y146 (= Y149) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K184) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A192) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D195) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E205) to H: in a breast cancer sample; somatic mutation
V219 (= V221) to G: in a breast cancer sample; somatic mutation
H231 (= H233) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
48% identity, 98% coverage: 5:315/317 of query aligns to 1:305/305 of 2hcrA

query
sites
2hcrA

S

N

I

I

M

K

V

I

F

F

S

S

G

G

N

S

A

S

N

H

K

Q

A

D

L

L

S

S

E

Q

G

K

I

I

V

A

R

D

K

R

L

L

N

G

M

L

R

E

L

L

G

G

M

K

A

V

S

V

V

T

D

K

R

K

F

F

S

S

D

N

G

Q

E

E

I

T

F

C

V

V

E

E

I

I

E

G

E

E

N

S

V

V

R

R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

C

C

S

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

A

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

S

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

V

V

A

A

D

N

M

M

I

L

G

S

H

V

A

A

G

G

A

A

D

D

R

H

A

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

V

D

D

N

N

V

L

Y

Y

A

A

S

E

P

P

I

A

L

V

L

L

G

K

D

W

V

I

W

R

R

E

Q

N

-

I

-

S

E

E

Y

W

E

R

N

N

L

C

I

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

R

R

L

L

G

-

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

D

R

R

A

-

N

-

V

-

S

-

E

-

I

-

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

K

G

D

R

R

T

V

C

A

V

I

M

L

V

V

D

D

L

M

V

A

D

D

T

T

A

C

G

G

T

T

L

I

C

C

H

H

A

A

S

D

A

K

L

L

K

L

K

S

N

A

G

G

A

A

V

T

K

R

V

V

V

Y

A

A

Y

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

A

P

A

A

A

I

D

S

N

R

V

I

R

N

N

N

S

A

V

C

L

F

D

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

T

E

D

D

E

K

L

M

K

K

A

H

I

C

G

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

E

M

M

I

L

L

A

A

E

E

T

A

I

I

R

R

I

T

A

H

V

N

G

G

E

E

S

S

V

V

S

S

S

Y

L

L

Y

F

binding adenosine monophosphate: R94 (= R98), Q95 (= Q99), K97 (≠ R101), H128 (= H133)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
47% identity, 98% coverage: 5:315/317 of query aligns to 2:305/309 of 8dbgA

query
sites
8dbgA

S

N

I

I

M

K

V

I

F

F

S

S

G

G

N

S

A

S

N

H

K

Q

A

D

L

L

S

S

E

Q

G

K

I

I

V

A

R

D

K

R

L

L

N

G

M

L

R

E

L

L

G

G

M

K

A

V

S

V

V

T

D

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

C

V

V

E

E

I

I

E

G

E

E

N
x
S

V

V

R
|
R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

C

C

S

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

A

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

S

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

V

V

A

A

D

N

M

M

I

L

G

S

H

V

A

A

G

G

A

A

D

D

R

H

A

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

V

D

D

N

N

V

L

Y

Y

A

A

S

E

P

P

I

A

L

V

L

L

G

K

D

W

V

I

W

R

R

E

Q

N

-

I

-

S

E

E

Y

W

E

R

N

N

L

C

I

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

R

R

L

L

G

-

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

R

-

A

-

N

-

V

-

S

-

E

D

I

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

K

G

D

R

R

T

V

C

A

V

I

M

L

V

V

D

D

L

M

V

A

D
|
D

T
|
T

A
x
C

G

G

T
|
T

L

I

C

C

H

H

A

A

S

D

A

K

L

L

K

L

K

S

N

A

G

G

A

A

V

T

K

R

V

V

V

Y

A

A

Y

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

A

P

A

A

A

I

D

S

N

R

V

I

R

N

N

N

S

A

V

C

L

F

D

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

T

E

D

D

E

K

L

M

K

K

A

H

I

C

G

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

E

M

M

I

L

L

A

A

E

E

T

A

I

I

R

R

I

T

A

H

V

N

G

G

E

E

S

S

V

V

S

S

S

Y

L

L

Y

F

binding adenosine-5'-triphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), R95 (= R98), Q96 (= Q99), K98 (≠ R101), H129 (= H133)
binding phosphate ion: S46 (≠ N49), R48 (= R51), D216 (= D226), T217 (= T227), C218 (≠ A228), T220 (= T230)

7yk1A Structural basis of human prps2 filaments (see paper)
46% identity, 98% coverage: 5:315/317 of query aligns to 2:303/306 of 7yk1A

query
sites
7yk1A

S

N

I

I

M

V

V

L

F

F

S

S

G

G

N

S

A

S

N

H

K

Q

A

D

L

L

S

S

E

Q

G

R

I

V

V

A

R

D

K

R

L

L

N

G

M

L

R

E

L

L

G

G

M

K

A

V

S

V

V

T

D

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

S

V

V

E

E

I

I

E

G

E

E

N
x
S

V

V

R
|
R

G

G

R

E

D

D

V

V

F

Y

V

I

Q

Q

P

S

T

G

C

C

S

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q

Q

D

D

R

K

R
x
K

S
x
D

R
x
K

S
|
S

A

-

R
|
R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

V

V

A

A

D

N

M

M

I

L

G

S

H

V

A

A

G

G

A

A

D

D

R

H

A

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

G

D

I

I

P

P

V

V

D
|
D

N

N

V

L

Y

Y

A

A

S

E

P

P

I

A

L

V

L

L

G

Q

D

W

V

I

W

R

R

E

Q

N

-

I

-

A

E

E

Y

W

E

K

N

N

L

C

I

I

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

R

R

L

L

G

-

D

N

A

V

D

E

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

R

-

A

-

N

-

V

-

S

-

E

-

I

-

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

K

G

D

R

R

T

V

C

A

V

I

M

L

V

V

D

D

L

M

V

A

D
|
D

T

T

A
x
C

G

G

T
|
T

L

I

C

C

H

H

A

A

S

D

A

K

L

L

K

L

K

S

N

A

G

G

A

A

V

T

K

K

V

V

V

Y

A

A

Y

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

A

P

A

A

A

I

D

S

N

R

V

I

R

N

N

N

S

A

V

A

L

F

D

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

T

E

D

D

E

K

L

M

K

K

A

H

I

C

G

T

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

E

M

M

I

L

L

A

A

E

E

T

A

I

I

R

R

I

T

A

H

V

N

G

G

E

E

S
|
S

V

V

S
|
S

S

Y

L

L

Y
x
F

binding adenosine-5'-diphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), S46 (≠ N49), R48 (= R51), R95 (= R98), K99 (≠ R102), D100 (≠ S103), K101 (≠ R104), S102 (= S105), R103 (= R107), H129 (= H133), D142 (= D146), S298 (= S310), S300 (= S312), F303 (≠ Y315)
binding phosphate ion: D214 (= D226), C216 (≠ A228), T218 (= T230)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
48% identity, 99% coverage: 1:315/317 of query aligns to 1:316/321 of O94413

query
sites
O94413

M

M

R

A

D

S

A

N

S

S

I

I

M

K

V

I

F

F

S

A

G

G

N

N

A

S

N

H

K

P

A

E

L

L

S

A

E

E

G

K

I

V

V

A

R

R

K

R

L

I

N

G

M

L

R

S

L

L

G

G

M

K

A

V

S

A

V

V

D

V

R

Q

F

Y

S

S

D

N

G

R

E

E

I

T

F

S

V

V

E

T

I

I

E

G

E

E

N

S

V

V

R

R

G

D

R

E

D

D

V

V

F

F

V

I

V

L

Q

Q

P

T

T

G

C

C

S

G

P

S

T

I

N

N

E

D

N

H

L

L

M

M

E

E

L

L

V

I

M

M

I

I

D

N

A

A

L

C

R

R

R

S

A

A

S

S

A

A

A

R

R

R

I

I

T

T

A

A

V

I

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

S

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

T

A

A

R

R

L

L

V

V

A

A

D

N

M

M

I

L

G

Q

H

T

A

A

G

G

A

C

D

N

R

H

A

I

L

I

T

T

V

M

D

D

L

L

H

H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

G

N

I

V

P

P

V

V

D

D

N

N

V

L

Y

Y

A

A

S

E

P

P

I

-

L

-

G

-

D

S

V

V

W

L

R

R

Q

Y

E

I

Y

R

E

E

N

N

L

I

-

D

-

T

-

V

-

N

-

P

-

T

I

V

V

I

V

V

S
|
S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

A

R

T

A

A

L

L

A

A

K

D

R

R

L

L

G

-

D

D

A

L

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

Q

R

K

A

A

N

N

V

E

S

V

E

S

I

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

D

R

G

D

R

K

T

L

C

A

V

I

M

L

V

V

D

D

L

M

V

A

D

D

T

T

A

C

G

G

T

T

L

L

C

G

H

L

A

A

S

K

A

T

L

L

K

K

K

D

N

N

G

G

A

A

V

K

K

A

V

V

V

Y

A

A

Y

I

C

V

T

T

H

H

P

G

V

I

L

L

S

S

G

G

A

K

A

A

A

I

D

K

N

V

V

I

R

N

N

E

S

S

V

A

L

L

D

E

E

K

L

V

V

I

V

V

T

T

D

N

T

T

I

I

P

P

L

H

T

D

D

D

E

K

L

R

K

S

A

L

I

C

G

S

K

K

I

I

R

E

Q

T

L

I

S

D

V

I

A

S

E

G

M

V

L

L

A

A

E

E

T

C

I

I

R

R

I

I

A

H

V

H

G

G

E

E

S

S

V

V

S

S

S

V

L

L

Y

F

S172 (= S170) modified: Phosphoserine

Query Sequence

>WP_013820404.1 NCBI__GCF_000214665.1:WP_013820404.1
MRDASIMVFSGNANKALSEGIVRKLNMRLGMASVDRFSDGEIFVEIEENVRGRDVFVVQP
TCSPTNENLMELLVMIDALRRASAARITAVIPYYGYARQDRRSRSARVPITARLVADMIG
HAGADRALTVDLHADQIQGFFGIPVDNVYASPILLGDVWRQEYENLIVVSPDVGGVVRAR
ALAKRLGDADLAIIDKRRPRANVSEIMHIIGDVDGRTCVMVDDLVDTAGTLCHAASALKK
NGAVKVVAYCTHPVLSGAAADNVRNSVLDELVVTDTIPLTDELKAIGKIRQLSVAEMLAE
TIRRIAVGESVSSLYVD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory