SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013821081.1 NCBI__GCF_000214665.1:WP_013821081.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
67% identity, 100% coverage: 2:1223/1223 of query aligns to 3:1224/1227 of P13009

query
sites
P13009

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C247 (= C246) binding Zn(2+)
C310 (= C309) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
C311 (= C310) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
E694 (= E691) binding methylcob(III)alamin
GDVHD 756:760 (= GDVHD 755:759) binding methylcob(III)alamin
D757 (= D756) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H758) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S803) binding methylcob(III)alamin
T808 (= T807) binding methylcob(III)alamin
S810 (= S809) mutation to A: Decreases activity by about 40%.
A860 (= A859) binding methylcob(III)alamin
D946 (= D944) binding S-adenosyl-L-methionine
R1134 (= R1133) binding S-adenosyl-L-methionine
YY 1189:1190 (≠ YF 1188:1189) binding S-adenosyl-L-methionine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
56% identity, 100% coverage: 7:1223/1223 of query aligns to 22:1262/1265 of Q99707

query
sites
Q99707

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Y

R61 (≠ S46) natural variant: R -> K
C255 (≠ S241) to Y: in dbSNP:rs1140598
GSR 382:384 (≠ GSA 366:368) binding (6S)-5,6,7,8-tetrahydrofolate
D449 (= D433) binding (6S)-5,6,7,8-tetrahydrofolate
N470 (= N454) binding (6S)-5,6,7,8-tetrahydrofolate
D537 (= D521) binding (6S)-5,6,7,8-tetrahydrofolate
N579 (= N563) binding (6S)-5,6,7,8-tetrahydrofolate
R585 (= R569) binding (6S)-5,6,7,8-tetrahydrofolate
R591 (= R575) binding (6S)-5,6,7,8-tetrahydrofolate
D919 (≠ R892) to G: in dbSNP:rs1805087
D963 (≠ H933) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (= K1033) mutation to N: Decreases binding to MTRR; when associated with E-963.

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
62% identity, 47% coverage: 650:1223/1223 of query aligns to 3:574/577 of 3bulA

query
sites
3bulA

E

E

W

W

R

R

E

S

W

W

P

E

V

V

S

N

K

K

R

R

L

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R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

M

E

P

P

F

F

M

I

D

E

A

A

E

S

V

K

D

E

G

Q

S

C

V

-

R

-

Q

K

T

T

N

N

G

G

K

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V
|
V

A

G

V

V

V

V

L

L

Q

Q

C

C

N

N

N

N

Y

Y

E

E

V

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

T

K

I

I

L

L

K

R

T

T

A

A

R

K

E

E

E

V

K

N

V

A

D

D

V

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

K

K

E

E

M

M

Q

E

R

R

Q

Q

G

G

F

F

T

T

I

I

P

P

L

L

M

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

R

K

A

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

N

N

Y

Y

Q

S

H

G

P

P

T

T

I

V

Y

Y

V

V

T
x
Q

D
x
N

A
|
A

S

S

R

R

S
x
T

V

V

G

G

V

V

V

V

S

A

A

A

L

L

L

L

S

S

E

D

D

T

Q

Q

K

R

A

D

E

D

F

F

V

V

E

A

K

R

T

T

R

R

A

K

E

E

Y

Y

E

E

Q

T

V

V

R

R

E

I

R

Q

H

H

K

G

G

R

R

K

H

K

A

P

K

R

N

T

P

P

Q

P

L

V

D

T

L

L

Q

E

K

A

A

A

R

R

H

D

N

N

K

D

F

F

-

A

-

F

D

D

F

W

A

Q

G

A

Y

Y

R

T

P

P

V

P

K

V

P

A

K

H

F

R

L

L

G

G

I

V

Q

Q

V

E

I

V

D

E

H

A

F

-

P

S

L

I

D

E

S

T

L

L

V

R

S

N

Y

Y

I

I

D

D

W

W

T

T

P

P

F

F

F

F

Q
x
M

T

T

W

W

E

S

L

L

S

A

G

G

R

K

Y

Y

P

P

A

R

I

I

L

L

S

E

D

D

Q

E

V

V

V

V

G

G

I

V

E

E

A

A

S

Q

K

R

L

L

F

F

A

K

D

D

A

A

Q

N

A

D

M

M

L

L

K

D

Q

K

L

L

V

S

D

A

E

E

Q

K

W

T

L

L

T

N

A

P

K

R

A

G

V

V

I

V

G

G

F

L

F

F

P

P

A

A

N

N

S

R

I

V

D

G

D

D

D

D

I

I

V

E

L

I

Y

Y

T

R

D

D

D

E

S

T

R

R

T

T

Q

H

Q

V

R

I

D

N

V

V

L

S

H

H

H

H

L

L

R

R

Q

Q

Q

Q

N

T

V

E

K

K

A

T

P

-

G

G

R

F

P

A

N

N

Y

Y

C

C

L

L

S

A

D

D

F

F

I

V

A

A

P

P

V

K

G

L

S

S

G

G

I

K

A

A

D

D

Y

Y

M

I

G

G

G

A

F

F

A

A

V

V

T

T

A

G

G

G

I

L

G

E

I

E

E

D

T

A

K

L

L

A

A

D

E

A

F

F

E

E

K

A

D

Q

H

H

D

D

D

D

Y

Y

S

N

S

K

I

I

M

M

L

V

K

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

C

Y

M

L

H

H

Q

E

K

R

V

V

R

R

K

K

E

V

Y

Y

W

W

G

G

Y

Y

A

A

A

P

E

N

E

E

T

N

H

L

D

S

N

N

H

E

D

E

L

L

I

I

E

R

E

E

A

N

Y

Y

Q

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P

P

A

A

C

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

A

A

K

T

L

I

F

W

E

E

L

L

L

L

N

E

V

V

T

E

E

K

S

H

T

T

T

G

I

M

E

K

L

L

T

T

E

E

N

S

F

F

A

A

M
|
M

Y

W

P

P

T
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

F

F

S

S

H

H

P

P

E

D

S

S

Q

K

Y

Y

F

Y

N

A

V

V

G

A

K

Q

I

I

D

Q

Q

R

D

D

Q

Q

L

V

Q

E

D

D

Y

Y

A

A

K

R

R

R

K

K

G

G

I

M

N

S

L

V

E

T

V

E

A

V

E

E

R

R

W

W

L

L

A

A

A

P

H

N

L

L

H

G

H

Y

active site: D107 (= D756), H109 (= H758), S160 (= S809)
binding cobalamin: H109 (= H758), V116 (= V765), G152 (= G801), L153 (= L802), S154 (= S803), L156 (= L805), I157 (= I806), T158 (= T807), G183 (= G832), G184 (= G833), Q208 (≠ T857), N209 (≠ D858), A210 (= A859), T213 (≠ S862), M302 (≠ Q950), D443 (= D1092), A486 (= A1135), P487 (= P1136), G488 (= G1137), Y489 (= Y1138), H495 (= H1144), K498 (= K1147), M521 (= M1170), G524 (≠ T1173), V527 (= V1176), S528 (= S1177)

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
62% identity, 47% coverage: 650:1223/1223 of query aligns to 3:574/576 of 3ivaA

query
sites
3ivaA

E

E

W

W

R

R

E

S

W

W

P

E

V

V

S

N

K

K

R

R

L

L

E

E

H

Y

A

S

L

L

V

V

K

K

G

G

I

I

A

T

D

E

Y

F

I

I

D

E

E

Q

D

D

T

T

E

E

Q

E

A

A

L

R

Q

Q

E

Q

A

A

E

T

K

R

P

P

L

C

H

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

V

V

G

G

D

D

L

L

F

F

G

G

E

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

M

E

P

P

F

F

M

I

D

E

A

A

E

S

V

K

D

E

G

Q

S

C

V

-

R

-

Q

K

T

T

N

N

G

G

K

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

A

G

V

V

V

V

L

L

Q

Q

C

C

N

N

N

N

Y

Y

E

E

V

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

T

K

I

I

L

L

K

R

T

T

A

A

R

K

E

E

E

V

K

N

V

A

D

D

V

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

K

K

E

E

M

M

Q

E

R

R

Q

Q

G

G

F

F

T

T

I

I

P

P

L

L

M

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

R

K

A

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

N

N

Y

Y

Q

S

H

G

P

P

T

T

I

V

Y

Y

V

V

T
x
Q

D
x
N

A

A

S

S

R

R

S

T

V

V

G

G

V

V

V

V

S

A

A

A

L

L

L

L

S

S

E

D

D

T

Q

Q

K

R

A

D

E

D

F

F

V

V

E

A

K

R

T

T

R

R

A

K

E

E

Y

Y

E

E

Q

T

V

V

R

R

E

I

R

Q

H

H

K

G

G

R

R

K

H

K

A

P

K

R

N

T

P

P

Q

P

L

V

D

T

L

L

Q

E

K

A

A

A

R

R

H

D

N

N

K

D

F

F

-

A

-

F

D

D

F

W

A

Q

G

A

Y

Y

R

T

P

P

V

P

K

V

P

A

K

H

F

R

L

L

G

G

I

V

Q

Q

V

E

I

V

D

E

H

A

F

-

P

S

L

I

D

E

S

T

L

L

V

R

S

N

Y

Y

I

I

D

D

W

W

T

T

P

P

F

F

F

F

Q

M

T
|
T

W

W

E

S

L

L

S

A

G

G

R

K

Y

Y

P

P

A

R

I

I

L

L

S

E

D

D

Q

E

V

V

V

V

G

G

I

V

E

E

A

A

S

Q

K

R

L

L

F

F

A

K

D

D

A

A

Q

N

A

D

M

M

L

L

K

D

Q

K

L

L

V

S

D

A

E

E

Q

K

W

T

L

L

T

N

A

P

K

R

A

G

V

V

I

V

G

G

F

L

F

F

P

P

A

A

N

N

S

R

I

V

D

G

D

D

D

D

I

I

V

E

L

I

Y

Y

T

R

D

D

D

E

S

T

R

R

T

T

Q

H

Q

V

R

I

D

N

V

V

L

S

H

H

H

H

L

L

R

R

Q

Q

Q

Q

N

T

V

E

K

K

A

T

P

-

G

G

R

F

P

A

N

N

Y

Y

C

C

L

L

S

A

D

D

F

F

I

V

A

A

P

P

V

K

G

L

S

S

G

G

I

K

A

A

D

D

Y

Y

M

I

G

G

G

A

F

F

A

A

V

V

T

T

A

G

G

G

I

L

G

E

I

E

E

D

T

A

K

L

L

A

A

D

E

A

F

F

E

E

K

A

D

Q

H

H

D

D

D

D

Y

Y

S

N

S

K

I

I

M

M

L

V

K

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E
|
E

A

A

F

F

A

A

E

E

C

Y

M

L

H

H

Q

E

K

R

V

V

R

R

K

K

E

V

Y

Y

W

W

G

G

Y

Y

A

A

A

P

E

N

E

E

T

N

H

L

D

S

N

N

H

E

D

E

L

L

I

I

E

R

E

E

A

N

Y

Y

Q

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G
|
G

Y
|
Y

P

P

A
|
A

C

C

P

P

D

E

H
|
H

T

T

E

E

K

K

A

A

K

T

L

I

F

W

E

E

L

L

L

L

N

E

V

V

T

E

E

K

S

H

T

T

T

G

I

M

E

K

L

L

T

T

E

E

N

S

F

F

A
|
A

M
|
M

Y

W

P

P

T
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

F

F

S

S

H

H

P

P

E

D

S

S

Q

K

Y
|
Y

F

Y

N

A

V

V

G

A

K

Q

I

I

D

Q

Q

R

D

D

Q

Q

L

V

Q

E

D

D

Y

Y

A

A

K

R

R

R

K

K

G

G

I

M

N

S

L

V

E

T

V

E

A

V

E

E

R

R

W

W

L

L

A

A

A

P

H

N

L

L

H

G

H

Y

active site: D107 (= D756), H109 (= H758), S160 (= S809)
binding cobalamin: H109 (= H758), G112 (= G761), V116 (= V765), G152 (= G801), L153 (= L802), S154 (= S803), L156 (= L805), I157 (= I806), T158 (= T807), G183 (= G832), G184 (= G833), Q208 (≠ T857), N209 (≠ D858), T303 (= T951), D443 (= D1092), A486 (= A1135), G488 (= G1137), Y489 (= Y1138), H495 (= H1144), A520 (= A1169), M521 (= M1170), G524 (≠ T1173), V527 (= V1176), S528 (= S1177)
binding s-adenosyl-l-homocysteine: E447 (= E1096), R484 (= R1133), P485 (= P1134), Y489 (= Y1138), A491 (= A1140), Y539 (= Y1188)

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
56% identity, 51% coverage: 7:635/1223 of query aligns to 6:611/611 of 4cczA

query
sites
4cczA

L

I

N

N

Q

A

Q

I

L

L

K

Q

Q

K

R

R

I

I

L

M

Y

V

L

L

D

D

G

G

A

G

M

M

G

G

T

T

M

M

I

I

Q

Q

S

R

Y

E

K

K

L

L

G

-

E

E

K

E

D

H

Y

F

R

R

G

G

T

Q

R

E

F

F

A

K

D

D

W

H

T

A

S

R

D

P

L

L

K

K

G

G

N

N

N

N

D

D

L

I

L

L

S

S

L

I

T

T

Q

Q

P

P

D

D

I

V

I

I

K

Y

A

Q

I

I

H

H

K

K

A

E

Y

Y

L

L

E

L

A

A

G

-

S

-

D

D

I

I

I

I

E

E

T

T

N

N

T

T

F

F

N

S

A

S

T

T

K

S

I

I

A

A

M

Q

A

A

D

D

Y

Y

H

G

M

L

E

E

D

H

L

L

A

A

Y

Y

E

R

I

M

N

N

V

M

A

C

S

S

A

A

R

G

L

V

A

A

K

R

Q

K

A

A

A

A

E

E

E

E

I

V

S

T

A

L

L

Q

T

T

P

G

D

I

K

K

P

-

R

R

F

F

V

V

A

A

G

G

V

A

L

L

G

G

P

P

T

T

N

N

R

K

T

T

S

L

S

S

M

V

S

S

P

P

D

S

V

V

N

E

D

R

P

P

G

D

F

Y

R

R

N

N

I

I

T

T

F

F

D

D

D

E

L

L

V

V

D

E

A

A

Y

Y

S

Q

E

E

A

Q

T

A

Q

K

G

G

L

L

I

L

D

D

G

G

G

G

A

V

D

D

I

I

I

L

L

L

I

I

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T

T

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I

F

F

D

D

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A

N

N

A

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K

K

A

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F

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N

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C

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N

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W

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N

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K

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T

-

A

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M

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L

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G

G

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P

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Y

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N

F

F

V

V

N

N

V

I

G

G

E
|
E

R

R

T

C

N

N

V

V

T

A

G
|
G

S

S

A
x
R

I

K

F

F

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A

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K

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L

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M

D

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|
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|
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|
D

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|
G

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|
S

N
|
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|
F

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|
R

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|
R

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Y

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A

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K

A

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G

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M

M

D

D

M

M

G

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N

N

A

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N

L

L

A

P

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V

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Y

A

D

D

D

I

I

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C

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D

D

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W

N

N

R

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P

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E

G

A

T

T

E

E

K

K

L

L

L

L

E

R

I

Y

A

A

E

Q

binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E360), G342 (= G366), R344 (≠ A368), N430 (= N454), M458 (= M482), D497 (= D521), G536 (= G560), S538 (= S562), N539 (= N563), F542 (= F566), R545 (= R569), R551 (= R575)

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
36% identity, 70% coverage: 9:858/1223 of query aligns to 7:820/841 of 8g3hA

query
sites
8g3hA

Q

R

Q

A

L

L

K

S

Q

Q

R

R

I

V

L

L

Y

V

L

F

D

D

G

G

A

A

M

M

G

G

T

T

M

E

I

L

Q

Q

S

K

Y

R

K

D

L

L

G

T

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P

K

E

D

D

Y

Y

R

G

G

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T

E

R

A

F

Y

A

-

D

-

W

-

T

-

S

-

D

-

L

-

K

Y

G

G

N

C

N

P

D

E

L

V

L

L

S

N

L

R

T

T

Q

R

P

P

D

D

I

V

I

V

K

R

A

E

I

I

H

H

K

L

A

S

Y

Y

L

L

E

E

A

A

G

G

S

A

D

E

I

V

I

I

E

E

T

T

N

N

T

S

F

F

N

G

A

A

T

L

K

R

I

H

A

V

M

L

A

A

D

E

Y

Y

H

G

M

L

E

G

D

E

L

E

A

A

Y

E

E

E

I

L

N

A

V

Y

A

R

S

A

A

A

R

R

L

I

A

A

K

K

Q

E

A

A

A

A

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E

E

P

I

Y

S

G

A

A

L

-

T

-

P

-

D

-

K

-

P

-

R

-

F

F

V

V

A

A

G

G

V

A

L

L

G

G

P

P

T

G

N

T

R

K

T

L

S

V

S

S

M

L

S

G

P

-

D

-

V

-

N

-

D

-

P

-

G

-

F

-

R

-

N

Q

I

I

T

S

F

W

D

E

D

E

L

L

V

F

D

S

A

A

Y

Y

S

K

E

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A

A

T

V

Q

R

G

G

L

L

I

V

D

R

G

G

G

G

A

V

D

D

I

L

I

I

L

L

I

L

E

E

T

T

V

A

F

Q

D

D

T

I

L

L

N

Q

A

V

K

R

A

C

A

A

I

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F

L

A

A

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A

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R

N

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T

A

F

M

D

A

K

E

L

L

G

G

Y

V

K

E

L

L

P

P

V

L

M

Q

I

V

S

Q

G

V

T

T

I

F

T

-

D

E

A

A

S

T

G

G

R

T

T

M

L

L

S

V

G

G

Q

T

T

D

A

E

A

Q

A

A

F

A

W

L

T

A

S

A

L

L

K

E

H

S

V

L

K

P

P

I

I

D

S

V

I

V

G

G

F

M

N

N

C

C

A

A

L

T

G

G

A

P

Q

D

E

L

L

M

R

D

Q

A

Y

K

I

I

E

R

E

Y

L

F

S

A

N

Q

I

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A

S

D

T

T

R

Y

F

V

V

S

A

A

C

H

L

P

P

N

N

A

A

G

G

L

L

P

P

-

R

N

N

E

E

F

G

G

G

E

R

-

V

-

V

Y

Y

D

D

E

L

T

T

P

P

E

E

Q

E

M

L

A

A

A

R

E

W

L

H

A

L

D

K

W

F

A

V

A

T

S

E

G

Y

Y

G

L

V

N

N

I

A

I

V

G

G

G

G

C

C

C

C

G

G

T

T

S