SitesBLAST
Comparing WP_013834808.1 NCBI__GCF_000214825.1:WP_013834808.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), D453 (≠ E446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
- binding magnesium ion: F370 (= F363), D453 (≠ E446), M458 (= M451), Q461 (= Q454), N480 (= N473), H482 (≠ Y475), K533 (≠ P519)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M258), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A555)
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 5wj1A
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), M263 (= M255), L264 (= L256), G286 (= G278), R288 (= R280), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M258), D291 (= D283), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A555)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 5k6tA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H259), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G286 (= G278), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477)
5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 5k6rA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R284), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), G286 (= G278), R288 (= R280), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), G328 (= G320), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 1z8nA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K127), R161 (= R153), Y191 (= Y183), R194 (vs. gap), D291 (= D283), R292 (= R284), D312 (= D304), W489 (= W482), G569 (= G556)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473)
- binding thiamine diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 1yi1A
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D283), R292 (= R284), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), M263 (= M255), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 1yi0A
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D283), R292 (= R284), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), G328 (= G320), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 1yhzA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D283), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 1yhyA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D283), R292 (= R284), V486 (= V479), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 1ybhA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M258), D291 (= D283), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ S279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/583 of 5k3sA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R284), M485 (= M478), W489 (= W482), G569 (= G556)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), G286 (= G278), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 13:575/582 of 3ea4A
- active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (= A28), S37 (≠ A29), E58 (= E51), T81 (= T74), F120 (= F113), Q121 (= Q114), E122 (= E115), K170 (= K163), M265 (= M258), V292 (= V285), V399 (= V393), G425 (= G419), M427 (= M421), D452 (≠ E446), N479 (= N473), H481 (≠ Y475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (= W482), H557 (≠ P545)
- binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D283), R291 (= R284), W488 (= W482), S567 (≠ A555)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R153), G221 (= G212), G222 (= G213), G223 (= G214), T245 (= T238), L246 (= L239), M247 (= M240), L263 (= L256), G264 (= G257), M265 (= M258), H266 (= H259), G285 (= G278), R287 (= R280), D289 (= D282), R291 (= R284), D309 (= D302), I310 (= I303), G327 (= G320), D328 (≠ P321), V329 (= V322), M404 (= M398), G422 (= G416)
- binding magnesium ion: D452 (≠ E446), N479 (= N473), H481 (≠ Y475)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V393), G400 (= G394), Q401 (= Q395), H402 (= H396), M427 (= M421), G451 (= G445), D452 (≠ E446), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ Y475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)
3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 13:575/582 of 3e9yA
- active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (= A28), S37 (≠ A29), E58 (= E51), T81 (= T74), F120 (= F113), Q121 (= Q114), E122 (= E115), K170 (= K163), M265 (= M258), V292 (= V285), V399 (= V393), G425 (= G419), M427 (= M421), D452 (≠ E446), N479 (= N473), H481 (≠ Y475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (= W482), H557 (≠ P545)
- binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D283), R291 (= R284), W488 (= W482), S567 (≠ A555)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R153), G221 (= G212), G222 (= G213), G223 (= G214), T245 (= T238), L246 (= L239), M247 (= M240), L263 (= L256), G285 (= G278), R287 (= R280), D289 (= D282), R291 (= R284), D309 (= D302), I310 (= I303), G327 (= G320), D328 (≠ P321), V329 (= V322), M404 (= M398), G422 (= G416)
- binding magnesium ion: D452 (≠ E446), N479 (= N473), H481 (≠ Y475)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V393), G400 (= G394), Q401 (= Q395), H402 (= H396), M427 (= M421), G451 (= G445), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ Y475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)
P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
44% identity, 99% coverage: 5:563/565 of query aligns to 99:661/670 of P17597
- A122 (= A28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- M124 (≠ L30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
- E144 (= E51) binding thiamine diphosphate
- S186 (= S93) binding FAD
- P197 (= P104) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
- R199 (≠ S106) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- Q207 (= Q114) binding thiamine diphosphate
- K220 (= K127) binding (R)-imazaquin
- R246 (= R153) binding (R)-imazaquin; binding FAD
- K256 (= K163) binding chlorimuron-ethyl
- G308 (= G213) binding FAD
- TL 331:332 (= TL 238:239) binding FAD
- C340 (≠ A247) modified: Cysteine sulfinic acid (-SO2H)
- LGMH 349:352 (= LGMH 256:259) binding FAD
- GVRFD 371:375 (≠ GSRFD 278:282) binding FAD
- DR 376:377 (= DR 283:284) binding chlorimuron-ethyl
- DI 395:396 (= DI 302:303) binding FAD
- DV 414:415 (≠ PV 321:322) binding FAD
- QH 487:488 (= QH 395:396) binding thiamine diphosphate
- GG 508:509 (= GG 416:417) binding FAD
- GAM 511:513 (≠ GTM 419:421) binding thiamine diphosphate
- D538 (≠ E446) binding Mg(2+)
- DGS 538:540 (≠ EGS 446:448) binding thiamine diphosphate
- N565 (= N473) binding Mg(2+)
- NQHLGM 565:570 (≠ NGYLGM 473:478) binding thiamine diphosphate
- H567 (≠ Y475) binding Mg(2+)
- W574 (= W482) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
- S653 (≠ A555) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/585 of 5k2oA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P545)
- binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M258), R292 (= R284), W489 (= W482), S568 (≠ A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G286 (= G278), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416)
- binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
44% identity, 99% coverage: 5:563/565 of query aligns to 14:576/582 of 7tzzA
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M258), R292 (= R284), W489 (= W482), S568 (≠ A555)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), D453 (≠ E446), G454 (= G447), S455 (= S448), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
- binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), M263 (= M255), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), R288 (= R280), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
- binding magnesium ion: A37 (= A28), T82 (= T74), S83 (= S75), Q122 (= Q114), Y381 (≠ A374), D453 (≠ E446), M458 (= M451), Q461 (= Q454), N480 (= N473), H482 (≠ Y475), K533 (≠ P519)
P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
43% identity, 99% coverage: 5:563/565 of query aligns to 96:658/667 of P09342
- C161 (≠ V71) modified: Disulfide link with 307
- P194 (= P104) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
- C307 (≠ V215) modified: Disulfide link with 161
P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
43% identity, 99% coverage: 5:563/565 of query aligns to 93:655/664 of P09114
- P191 (= P104) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
- W568 (= W482) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
43% identity, 99% coverage: 3:564/565 of query aligns to 14:578/599 of 6denA
- active site: Y35 (= Y24), G37 (= G26), G38 (= G27), A39 (= A28), I40 (≠ A29), E61 (= E51), T84 (= T74), F123 (= F113), Q124 (= Q114), E125 (= E115), K173 (= K163), K230 (≠ E223), M266 (= M258), V293 (= V285), V409 (= V393), L434 (= L418), G435 (= G419), M437 (= M421), D462 (≠ E446), N489 (= N473), E491 (≠ Y475), Q492 (≠ L476), M494 (= M478), V495 (= V479), W498 (= W482), L520 (= L505), N525 (≠ G510), V526 (≠ H511)
- binding flavin-adenine dinucleotide: R163 (= R153), G219 (= G212), A220 (≠ G213), G221 (= G214), N224 (≠ L217), T246 (= T238), L247 (= L239), Q248 (≠ M240), L264 (= L256), G286 (= G278), A287 (≠ S279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), E319 (≠ D302), I320 (= I303), N324 (≠ S307), D338 (≠ Q324), V339 (= V325), M414 (= M398), G432 (= G416)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (= M258), D291 (= D283), R292 (= R284), W498 (= W482)
- binding magnesium ion: D462 (≠ E446), N489 (= N473), E491 (≠ Y475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (= V393), G410 (= G394), Q411 (= Q395), H412 (= H396), G435 (= G419), M437 (= M421), G461 (= G445), D462 (≠ E446), A463 (≠ G447), S464 (= S448), N489 (= N473), E491 (≠ Y475), Q492 (≠ L476), G493 (= G477), M494 (= M478), V495 (= V479)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
43% identity, 99% coverage: 3:564/565 of query aligns to 12:576/597 of 6demA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (≠ A29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), K228 (≠ E223), M264 (= M258), V291 (= V285), V407 (= V393), L432 (= L418), G433 (= G419), M435 (= M421), D460 (≠ E446), N487 (= N473), E489 (≠ Y475), Q490 (≠ L476), M492 (= M478), V493 (= V479), W496 (= W482), L518 (= L505), N523 (≠ G510), V524 (≠ H511)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (= M258), D289 (= D283), R290 (= R284), M492 (= M478), W496 (= W482), A567 (= A555)
- binding flavin-adenine dinucleotide: R161 (= R153), G217 (= G212), A218 (≠ G213), G219 (= G214), N222 (≠ L217), T244 (= T238), L245 (= L239), Q246 (≠ M240), L262 (= L256), G284 (= G278), A285 (≠ S279), R286 (= R280), D288 (= D282), R290 (= R284), V291 (= V285), E317 (≠ D302), I318 (= I303), N322 (≠ S307), D336 (≠ Q324), V337 (= V325), M412 (= M398), G430 (= G416)
- binding magnesium ion: D460 (≠ E446), N487 (= N473), E489 (≠ Y475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V393), G408 (= G394), Q409 (= Q395), H410 (= H396), M435 (= M421), G459 (= G445), D460 (≠ E446), A461 (≠ G447), S462 (= S448), M465 (= M451), N487 (= N473), E489 (≠ Y475), Q490 (≠ L476), G491 (= G477), M492 (= M478), V493 (= V479)
Query Sequence
>WP_013834808.1 NCBI__GCF_000214825.1:WP_013834808.1
MELTGAQILVHFLEDEGVEHIWGYPGGAALPIYDALDTDAKELKHILVRHEQAAVHAADG
YARSTGKPGVVLVTSGPGATNTVTGIATAYMDSIPMIVFTGQVPTSLIGLDAFQEVDTVG
ITRPIVKHNFLVKDVKDLAITLKKAFYIATTGRPGPVVVDIPKDVQNATSTYVYTQEVEL
RSYLPVTKGHSGQIKKAVELMLSAERPIFYTGGGVVLGDASEELVSLVKSLGYPITQTLM
GLGAYPAQDQQNLGMLGMHGTYEANLAMHHCDVLIAIGSRFDDRVTGNIEKFCPTAKIIH
VDIDPASISKNVMVDVPIVGPVKQVVAEMNQHLAKLEKGRNKEALDAWWAQIAEWREMNC
LKFDESGTKIKPQAAVKAVWEVTKGDAYVTSDVGQHQMFAAQYYTFEKPRRWINSGGLGT
MGFGLPAAMGVQMALPDATVVCITGEGSIQMNIQELSTCLQYGLPIKIVSLNNGYLGMVR
QWQEFFYDRRYSMSYMDSLPDFVKLTESYGHVGVRIDDPKTMRQQLEEAFALKDRLVFID
ILTDPQENVYPMIPAGAGQNEMILV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory