SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013835658.1 NCBI__GCF_000214825.1:WP_013835658.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
80% identity, 99% coverage: 4:275/275 of query aligns to 3:273/274 of 3tdtA

query
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3tdtA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding 2-amino-6-oxopimelic acid: R104 (= R106), M124 (= M126), M139 (= M141), D141 (= D143)
binding coenzyme a: G185 (= G187), A186 (= A188), S203 (= S205), M204 (= M206), R217 (= R219), V234 (= V236), K254 (= K256), K259 (= K261), T260 (= T262), K263 (= K265)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
80% identity, 99% coverage: 4:275/275 of query aligns to 3:273/274 of 2tdtA

query
sites
2tdtA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding coenzyme a: S159 (= S161), F183 (= F185), G185 (= G187), S203 (= S205), M204 (= M206), R217 (= R219), K254 (= K256), K259 (= K261), T260 (= T262), K263 (= K265)
binding (2S)-2-aminoheptanedioic acid: R104 (= R106), M124 (= M126), M139 (= M141), D141 (= D143)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
80% identity, 99% coverage: 4:275/275 of query aligns to 3:273/274 of 1kgtA

query
sites
1kgtA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding pimelic acid: R104 (= R106), M124 (= M126), M139 (= M141)
binding succinyl-coenzyme a: D141 (= D143), S159 (= S161), A186 (= A188), S203 (= S205), R217 (= R219), K259 (= K261), T260 (= T262), K263 (= K265)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
80% identity, 99% coverage: 4:275/275 of query aligns to 3:273/274 of 1kgqA

query
sites
1kgqA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding (2S)-2-aminoheptanedioic acid: R104 (= R106), M124 (= M126), M139 (= M141), D141 (= D143)
binding succinamide-coa: F183 (= F185), G185 (= G187), S203 (= S205), M204 (= M206), R217 (= R219), V234 (= V236), K254 (= K256), K259 (= K261), T260 (= T262), K263 (= K265)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
38% identity, 36% coverage: 99:196/275 of query aligns to 70:168/203 of 3r8yA

query
sites
3r8yA

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A

K

P

P

T

V

I

I

I

V

E

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

S

V

E
x
V

V

V

V

L

E

E

G

G

V

V

active site: D115 (= D143), G140 (= G168), V163 (≠ E191)
binding calcium ion: G121 (= G149), G122 (≠ S150)

3r5bA Pseudomonas aeruginosa dapd (pa3666) in complex with l-2-aminopimelate (see paper)
28% identity, 59% coverage: 55:216/275 of query aligns to 135:284/321 of 3r5bA

query
sites
3r5bA

W

W

T

T

V

N

N

I

E

G

W

A

L

V

K

D

K

L

A

A

V

E

L

L

L

A

S

E

F

L

R

Q

T

L

E

E

D

A

N

R

V

L

V

K

M

G

N

K

S

L

S

L

E

E

T

V

R

F

Y

S

F

V

D

D

K

K

V

F

P

P

S

-

K

K

F

M

A

T

D

D

Y

Y

S

-

Q

-

A

-

D

-

F

V

E

V

A

P

A

A

G

G

V

V

R

R

V

I

V

A

P

D

P

T

A

A

M

R

A

V

R
|
R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

-

G

S

T

T

T

V

V

L

M

M

H

P

E

S

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M

M

V

I

D

E

T

G

W

R

V

V

T
x
S

V

A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

A

-

P

-

T

-

I

-

I

V

E

G

D

E

N

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

V

I

V

-

E

-

G

G

V

I

I

P

V

L

E

G

E

D

G

R

S

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

R

K

I

V

binding (2S)-2-aminoheptanedioic acid: R189 (= R114), S223 (≠ T147)

Sites not aligning to the query:

binding (2S)-2-aminoheptanedioic acid: 127

3r5aA Pseudomonas aeruginosa dapd (pa3666) in complex with d-2-aminopimelate (see paper)
28% identity, 59% coverage: 55:216/275 of query aligns to 135:284/321 of 3r5aA

query
sites
3r5aA

W

W

T

T

V

N

N

I

E

G

W

A

L

V

K

D

K

L

A

A

V

E

L

L

L

A

S

E

F

L

R

Q

T

L

E

E

D

A

N

R

V

L

V

K

M

G

N

K

S

L

S

L

E

E

T

V

R

F

Y

S

F

V

D

D

K

K

V

F

P

P

S

-

K

K

F

M

A

T

D

D

Y

Y

S

-

Q

-

A

-

D

-

F

V

E

V

A

P

A

A

G

G

V

V

R
|
R

V

I

V

A

P

D

P

T

A

A

M

R

A

V

R
|
R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

-

G

S

T

T

T

V

V

L
x
M

M

H

P

E

S

G

Y

F

V

V

N
|
N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M
|
M

V

I

D

E

T

G

W

R

V

V

T
x
S

V
x
A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

A

-

P

-

T

-

I

-

I

V

E

G

D

E

N

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

V

I

V

-

E

-

G

G

V

I

I

P

V

L

E

G

E

D

G

R

S

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

R

K

I

V

binding (2R)-2-aminoheptanedioic acid: R181 (= R106), R189 (= R114), M201 (≠ L125), N207 (= N131), M217 (= M141), S223 (≠ T147), A224 (≠ V148)

Sites not aligning to the query:

binding (2R)-2-aminoheptanedioic acid: 127

3r5cA Pseudomonas aeruginosa dapd (pa3666) in complex with coa and succinate (see paper)
27% identity, 59% coverage: 55:216/275 of query aligns to 138:285/338 of 3r5cA

query
sites
3r5cA

W

W

T

T

V

N

N

I

E

G

W

A

L

V

K

D

K

L

A

A

V

E

L

L

L

A

S

E

F

L

R

Q

T

L

E

E

D

A

N

R

V

L

V

K

M

G

N

K

S

L

S

L

E

E

T

V

R

F

Y

S

F

V

D

D

K

K

V

F

P

P

S

-

K

K

F

M

A

T

D

D

Y

Y

S

-

Q

-

A

-

D

-

F

V

E

V

A

P

A

A

G

G

V

V

R

R

V

I

V

A

P

D

P

T

A

A

M

R

A

V

R

R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

-

G

S

T

T

T

V

V

L

M

M

H

P

E

S

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M

M

V

I

D

E

T

G

W

R

V

V

T

S

V

A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G
x
M

G

G

V

T

L

I

E

V

P

-

L

-

Q

-

A

-

A

-

P

-

T

I

I

S

I

V

E

G

D

E

N

G

C

C

F

L

I

I

G
|
G

A
|
A

R

N

S

A

E

G

V

I

V

-

E

-

G

G

V

I

I

P

V

L

E

G

E

D

G

R

S

N

V

I

I

V

S
x
E

M
x
A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S
x
G

T

T

R
x
K

I

V

binding coenzyme a: M244 (≠ G167), G258 (= G187), A259 (= A188), E274 (≠ S205), A275 (≠ M206), G282 (≠ S213), K284 (≠ R215)

Sites not aligning to the query:

binding coenzyme a: 299, 312, 313, 314, 315

Query Sequence

>WP_013835658.1 NCBI__GCF_000214825.1:WP_013835658.1
MSNQLQLIIEAAFERRAEITPKNVDAETKQAIQETLHLLDSGKMRVAERQGVGQWTVNEW
LKKAVLLSFRTEDNVVMNSSETRYFDKVPSKFADYSQADFEAAGVRVVPPAMARRGSYIA
PSTVLMPSYVNIGAYVDSGTMVDTWVTVGSCAQIGKNVHLSGGVGIGGVLEPLQAAPTII
EDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVSGNL
PSKDGKYSLYCAVIVKKVDAKTLGKVGINELLRDI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory