SitesBLAST
Comparing WP_013835838.1 NCBI__GCF_000214825.1:WP_013835838.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
65% identity, 97% coverage: 12:416/416 of query aligns to 5:410/410 of P0A9T0
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1ybaA The active form of phosphoglycerate dehydrogenase (see paper)
65% identity, 97% coverage: 12:416/416 of query aligns to 1:406/406 of 1ybaA
- active site: N104 (= N115), R236 (= R247), D260 (= D271), E265 (= E276), H288 (= H299)
- binding 2-oxoglutaric acid: R56 (= R67), S57 (= S68), C79 (= C90), I80 (= I91)
- binding nicotinamide-adenine-dinucleotide: I80 (= I91), F102 (= F113), V108 (= V119), G154 (= G165), G156 (= G167), H157 (≠ R168), I158 (= I169), Y176 (= Y187), D177 (= D188), I178 (= I189), K181 (≠ Q192), H206 (= H217), V207 (= V218), P208 (= P219), A234 (= A245), S235 (≠ A246), R236 (= R247), H288 (= H299), G290 (= G301)
- binding phosphate ion: G81 (= G92), N83 (= N94)
2p9eA Crystal structure of g336v mutant of e.Coli phosphoglycerate dehydrogenase (see paper)
65% identity, 97% coverage: 12:416/416 of query aligns to 1:406/406 of 2p9eA
- active site: N104 (= N115), R236 (= R247), D260 (= D271), E265 (= E276), H288 (= H299)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G156 (= G167), H157 (≠ R168), I158 (= I169), Y176 (= Y187), D177 (= D188), I178 (= I189), H206 (= H217), V207 (= V218), P208 (= P219), S212 (≠ E223), A234 (= A245), S235 (≠ A246), R236 (= R247), H288 (= H299), G290 (= G301)
1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
65% identity, 97% coverage: 15:416/416 of query aligns to 2:404/404 of 1psdA
- active site: N102 (= N115), R234 (= R247), D258 (= D271), E263 (= E276), H286 (= H299)
- binding nicotinamide-adenine-dinucleotide: N102 (= N115), H155 (≠ R168), I156 (= I169), D175 (= D188), I176 (= I189), K179 (≠ Q192), H204 (= H217), V205 (= V218), P206 (= P219), A232 (= A245), S233 (≠ A246), R234 (= R247), H286 (= H299)
- binding serine: H338 (= H352), N340 (= N354), R341 (≠ Q355), V344 (≠ I358)
1sc6D Crystal structure of w139g d-3-phosphoglycerate dehydrogenase complexed with NAD+ (see paper)
61% identity, 97% coverage: 15:416/416 of query aligns to 2:384/384 of 1sc6D
- active site: N102 (= N115), R228 (= R247), D252 (= D271)
- binding nicotinamide-adenine-dinucleotide: P99 (= P112), F100 (= F113), N102 (= N115), T103 (= T116), G146 (= G165), G148 (= G167), H149 (≠ R168), I150 (= I169), Y168 (= Y187), D169 (= D188), I170 (= I189), H198 (= H217), V199 (= V218), P200 (= P219), S204 (≠ E223), T205 (= T224), S227 (≠ A246)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
48% identity, 97% coverage: 13:416/416 of query aligns to 51:466/466 of P87228
- S87 (= S50) modified: Phosphoserine
- S258 (≠ T221) modified: Phosphoserine
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 3:299/301 of 6rj5A
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 4:300/305 of 6plfA
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 3:299/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I164), G147 (= G165), L148 (≠ Y166), G149 (= G167), R150 (= R168), I151 (= I169), G152 (= G170), D170 (= D188), H201 (= H217), T202 (≠ V218), P203 (= P219)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 3:299/302 of 6rihA
7dkmA Phgdh covalently linked to oridonin (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 4:300/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ I91), A102 (≠ V119), G148 (= G165), R151 (= R168), I152 (= I169), Y170 (= Y187), D171 (= D188), P172 (vs. gap), I173 (vs. gap), H202 (= H217), T203 (≠ V218), P204 (= P219), T209 (= T224), C230 (≠ A245), A231 (= A246), R232 (= R247), H279 (= H299), G281 (= G301)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ G29), K17 (≠ A32), I18 (≠ Y33), E293 (≠ A313)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 3:299/303 of 6plgA
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
37% identity, 72% coverage: 20:320/416 of query aligns to 1:296/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G167), I148 (= I169), Y166 (= Y187), D167 (= D188), P168 (vs. gap), I169 (vs. gap), I170 (= I189), H198 (= H217), T199 (≠ V218), L208 (≠ M227), R228 (= R247)
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
37% identity, 73% coverage: 19:320/416 of query aligns to 2:298/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N115), A100 (≠ V119), R149 (= R168), I150 (= I169), Y168 (= Y187), D169 (= D188), P170 (vs. gap), I171 (vs. gap), H200 (= H217), T201 (≠ V218), P202 (= P219), T207 (= T224), C228 (≠ A245), A229 (= A246), R230 (= R247), H277 (= H299), G279 (= G301)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
37% identity, 75% coverage: 19:332/416 of query aligns to 8:315/533 of O43175
- T78 (≠ I91) binding NAD(+)
- R135 (≠ K148) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 168:169) binding NAD(+)
- D175 (= D188) binding NAD(+)
- T207 (≠ V218) binding NAD(+)
- CAR 234:236 (≠ AAR 245:247) binding NAD(+)
- D260 (= D271) binding NAD(+)
- V261 (= V272) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIGG 299:302) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
37% identity, 72% coverage: 19:319/416 of query aligns to 2:297/297 of 6rj3A
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
39% identity, 63% coverage: 58:320/416 of query aligns to 35:290/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R168), Y160 (= Y187), D161 (= D188), P162 (vs. gap), I164 (= I189), L179 (= L204), T193 (≠ V218), P194 (= P219), S198 (≠ E223), L202 (≠ M227)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
40% identity, 64% coverage: 52:316/416 of query aligns to 33:294/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
40% identity, 64% coverage: 52:316/416 of query aligns to 34:295/525 of 3ddnB
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
40% identity, 63% coverage: 58:320/416 of query aligns to 39:299/304 of 1wwkA
- active site: S96 (≠ N115), R230 (= R247), D254 (= D271), E259 (= E276), H278 (= H299)
- binding nicotinamide-adenine-dinucleotide: V100 (= V119), G146 (= G165), F147 (≠ Y166), G148 (= G167), R149 (= R168), I150 (= I169), Y168 (= Y187), D169 (= D188), P170 (= P194), V201 (= V218), P202 (= P219), T207 (= T224), T228 (≠ A245), S229 (≠ A246), D254 (= D271), H278 (= H299), G280 (= G301)
Query Sequence
>WP_013835838.1 NCBI__GCF_000214825.1:WP_013835838.1
MSENLTPQPAFSLAKEKINILLLEGIHPGAEAYFREQGYSNIISHAGALSPSELKTALQD
CHFIGIRSRTQLTAELLAQAPKLNAIGCFCIGTNQVDLTAAMRLGIPVFNAPFSNTRSVA
ELVLGEILLLLRRVPEKNALAHRSIWQKSAQGAFEARGKTLGIIGYGRIGSQLSILAENL
GMRVIFYDIAKQLPLNNAQQVASLAELLSKADVVSLHVPETPETEWMIGHQQLAMMKPGS
LLINAARGRVVDIPALADALQSGQLAGAAIDVFPTEPETNKQPFESVLRGLDNVILTPHI
GGSTEEAQASIGAEVAAKLVAYSDIGSSISAVNFPEVALPEHIGRSRLLHIHHNQPGILT
QINQAFAAQNINIAAQYLQTNSEIGYVVIDLDAKDRDAGLAQLKQIPGTLRTRLLH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory