SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013835916.1 NCBI__GCF_000214825.1:WP_013835916.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
62% identity, 99% coverage: 2:318/321 of query aligns to 3:315/318 of Q63XL8

query
sites
Q63XL8

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RQ 99:100 (= RQ 99:100) binding ATP

6asvC E. Coli prpp synthetase (see paper)
60% identity, 97% coverage: 8:318/321 of query aligns to 4:311/311 of 6asvC

query
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6asvC

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binding phosphate ion: D168 (= D176), D218 (= D225), D219 (= D226), D222 (= D229), T223 (= T230), G224 (≠ A231), T226 (= T233), L227 (= L234)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
60% identity, 97% coverage: 8:318/321 of query aligns to 6:313/315 of P0A717

query
sites
P0A717

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L

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D129 (= D132) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D225) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D226) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D229) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
60% identity, 96% coverage: 8:314/321 of query aligns to 5:308/308 of 4s2uA

query
sites
4s2uA

I

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I

binding magnesium ion: D223 (= D229), T227 (= T233)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
63% identity, 95% coverage: 5:309/321 of query aligns to 2:298/299 of 6nfeB

query
sites
6nfeB

S

T

V

M

M

M

I

I

F

F

A

T

G

G

N

N

A

A

N

N

V

P

T

E

L

L

A

A

E

L

R

K

I

I

S

S

L

S

Y

H

L

L

D

Q

Q

I

P

P

L

I

G

G

Q

K

A

A

N

T

V

V

G

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

M

M

V

V

Q

E

I

I

T

L

E

E

S

N

V

V

R

R

G

G

K

K

D

D

V

V

Y

F

V

V

L

L

Q

Q

P

S

T

T

C

C

T

A

P

P

E

A

P

N

A

N

V

-

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

K

G

R

R

I

I

T

T

A

A

V

V

P

P

Y

Y

Y

F

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

P

V

H

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

A

V

A

A

D

D

M

M

I

M

T

A

V

S

A

V

G

G

A

I

D

C

R

R

M

V

I

L

T

T

I

V

D

D

L

L

H
|
H

S

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

I

V

Y

Y

A

S

S

T

P

P

V

V

L

L

V

L

E

E

D

D

M

I

L

T

A

K

Q

Q

S

-

G

-

S

-

E

K

V

L

S

N

N

N

I

I

A

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

A

A

V

V

A

A

K

K

A

R

L

L

-

N

D

D

A

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

S

Q

E

V

V

M

M

N

H

V

I

I

I

G

G

D

E

V

P

R

A

G

N

R

K

D

N

C

C

I

I

V

V

D
|
D

M
x
I

V

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

C

C

K

T

A

A

A

H

A

E

L

L

M

K

E

K

N

N

G

G

A

A

K

K

S

S

V

V

T

R

A

A

Y

Y

A

I

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

V

V

E

N

N

N

I

I

R

K

K

H

S

S

K

G

L

L

K

D

E

E

L

V

V

V

T

T

D

D

T

T

I

I

P

P

Q

L

S

S

L

A

D

E

A

A

M

Q

E

N

C

C

E

E

K

K

I

I

R

R

Q

V

V

V

S

S

V

L

A

A

N

D

I

M

L

L

G

A

E

Q

T

A

I

I

R

K

R

R

V

V

N

N

binding adenosine-5'-diphosphate: F34 (= F37), D36 (= D39), E38 (= E41), R95 (= R99), Q96 (= Q100), H130 (= H134)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H134), D214 (= D225), D215 (= D226), I216 (≠ M227), D218 (= D229), T219 (= T230), A220 (= A231), T222 (= T233)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
63% identity, 95% coverage: 5:309/321 of query aligns to 2:297/298 of 6nfeA

query
sites
6nfeA

S

T

V

M

M

M

I

I

F

F

A

T

G

G

N

N

A

A

N

N

V

P

T

E

L

L

A

A

E

L

R

K

I

I

S

S

L

S

Y

H

L

L

D

Q

Q

I

P

P

L

I

G

G

Q

K

A

A

N

T

V

V

G

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

M

M

V

V

Q

E

I

I

T

L

E

E

S

N

V

V

R

R

G

G

K

K

D

D

V

V

Y

F

V

V

L

L

Q

Q

P

S

T

T

C

C

T

A

P

P

E

A

P

N

A

N

V

-

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

K

G

R

R

I

I

T

T

A

A

V

V

P

P

Y

Y

Y

F

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

P
x
V

H

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

A

V

A

A

D

D

M

M

I

M

T

A

V

S

A

V

G

G

A

I

D

C

R

R

M

V

I

L

T

T

I

V

D

D

L

L

H
|
H

S

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

I

V

Y
|
Y

A

S

S

T

P

P

V

V

L

L

V

L

E

E

D

D

M

I

L

T

A

K

Q

Q

S

-

G

-

S

-

E

K

V

L

S

N

N

N

I

I

A

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R
|
R

A

A

R

R

A
|
A

V

V

A

A

K
|
K

A

R

L

L

-

N

D

D

A

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

Q

E

V

V

M

M

N

H

V

I

I

I

G

G

D

E

V

P

R

A

G

N

R

K

D

N

C

C

I

I

V

V

D
|
D

M
x
I

V

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

C

C

K

T

A

A

A

H

A

E

L

L

M

K

E

K

N

N

G

G

A

A

K

K

S

S

V

V

T

R

A

A

Y

Y

A

I

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

V

V

E

N

N

N

I

I

R

K

K

H

S

S

K

G

L

L

K

D

E

E

L

V

V

V

T

T

D

D

T

T

I

I

P

P

Q

L

S

S

L

A

D

E

A

A

M

Q

E

N

C

C

E

E

K

K

I

I

R

R

Q

V

V

V

S

S

V

L

A

A

N

D

I

M

L

L

G

A

E

Q

T

A

I

I

R

K

R

R

V

V

N

N

binding adenosine-5'-diphosphate: F34 (= F37), D36 (= D39), E38 (= E41), R95 (= R99), Q96 (= Q100), H130 (= H134)
binding adenosine monophosphate: R98 (= R102), V100 (≠ P104), Y146 (= Y150), R175 (= R182), A178 (= A185), K181 (= K188)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H134), D213 (= D225), D214 (= D226), I215 (≠ M227), D217 (= D229), T218 (= T230), A219 (= A231), T221 (= T233)

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
61% identity, 97% coverage: 5:314/321 of query aligns to 1:299/300 of 3dahC

query
sites
3dahC

S

G

V

L

M

M

I

V

F

F

A

T

G

G

N

N

A

A

N

N

V

P

T

A

L

L

A

A

E

Q

R

E

I

V

S

V

L

K

Y

I

L

L

D

G

Q

I

P

P

L

L

G

G

Q

K

A

A

N

M

V

V

G

S

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

I

M

Q

V

V

Q

E

I

I

T

Q

E

E

S

N

V

V

R

R

G

G

K

K

D

D

V

V

Y

F

V

V

L

L

Q

Q

P

S

T

T

C

C

T

A

P

P

E

T

P

N

A

D

V

-

N

N

L

L

M

M

E

E

M

L

L

M

V

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

G

R

R

I

I

T

T

A

A

V

A

V

I

P

P

Y

Y

Y

F

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

P

P

H

R

S

S

A

A

R

R

V

V

P

A

I

I

T

S

A

A

R

K

L

V

A

V

A

A

D

N

M

M

I

L

T

E

V

I

A

A

G

G

A

V

D

E

R

R

M

I

I

I

T

T

I

M

D

D

L

L

H

H

S

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

I

Y

Y

A

A

S

T

P

P

V

I

L

L

V

L

E

G

D

D

M

L

L

R

A

K

Q

Q

S

N

G

-

S

-

E

-

V

Y

S

P

N

D

I

L

A

L

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

A

A

V

L

A

A

K

K

A

Q

L

L

D

N

A

C

D

D

L

L

A

A

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

Q

-

V

V

M

M

N

N

V

I

I

I

G

G

D

E

V

V

R

E

G

G

R

R

D

T

C

C

I

V

I

I

V

M

D

D

M

M

V

V

D

D

T

T

A

A

G

G

T

T

L

L

C

C

K

K

A

A

A

Q

A

V

L

L

M

K

E

E

N

R

G

G

A

A

K

K

S

Q

V

V

T

F

A

A

Y

Y

A

A

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

G

A

A

V

A

E

D

N

R

I

I

R

A

K

A

S

S

K

A

L

L

K

D

E

E

L

L

V

V

T

T

D

D

T

T

I

I

P

P

Q

L

S

S

L

A

D

E

A

S

M

L

E

A

C

C

E

P

K

K

I

I

R

R

Q

A

V

L

S

S

V

S

A

A

N

G

I

L

L

L

G

A

E

E

T

T

I

F

R

S

R

R

V

I

N

R

E

G

E

D

S

S

V

V

binding adenosine monophosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R99), Q95 (= Q100)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
58% identity, 97% coverage: 8:318/321 of query aligns to 4:305/307 of 7xmvA

query
sites
7xmvA

I

L

F

F

A

A

G

G

N

N

A

A

N

T

V

P

T

E

L

L

A

A

E

Q

R

R

I

I

S

A

L

N

Y

R

L

L

D

Y

Q

T

P

S

L

L

G

G

Q

D

A

A

N

A

V

V

G

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

M

S

V

V

Q

Q

I

I

T

N

E

E

S

N

V

V

R

R

G

G

K

G

D

D

V

I

Y

F

V

I

L

I

Q

Q

P

S

T

T

C

C

T

A

P

P

E

T

P

N

A

D

V

-

N

N

L

L

M

M

E

E

M

L

L

V

V

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

K

G

R

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

Y

F

G

G

F

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

R

R

P
x
V

H
x
R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

A

V

A

A

D

D

M

F

I

L

T

S

V

S

A

V

G

G

A

V

D

D

R

R

M

V

I

L

T

T

I

V

D

D

L

L

H
|
H

S

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

I

V

Y
x
F

A

G

S
|
S

P

P

V

I

L

L

V

L

E

E

D

D

M

M

L

L

A

Q

Q

L

S

N

G

-

S

-

E

-

V

L

S

D

N

N

I

P

A

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A
|
A

V

I

A

A

K

K

A

L

L

L

-

N

D

D

A

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

Q

Q

V

V

M

M

N

H

V

I

I

I

G

G

D

D

V

V

R

A

G

G

R

R

D

D

C

C

I

V

I

L

V

V

D
|
D

M
|
M

V

I

D
|
D

T
|
T

A

G

G
|
G

T
|
T

L

L

C

C

K

K

A

A

A

E

A

A

L

L

M

K

E

E

N

R

G

G

A

A

K

K

S

R

V

V

T

F

A

A

Y

Y

A

A

T

T

H

H

A

P

V

I

L

F

S

S

G

G

P

N

A

A

V

A

E

N

N

N

I

L

R

R

K

N

S

S

K

V

L

I

K

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

Q

L

S

S

L

D

D

E

A

I

M

K

E

S

C

L

E

P

K

N

I

V

R

R

Q

T

V

L

S

T

V

L

A

S

N

G

I

M

L

L

G

A

E

E

T

A

I

I

R

R

V

I

N

S

N

N

E

E

S

S

V

I

T

S

A

A

L

M

M

F

binding adenosine-5'-diphosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R99), R97 (= R102), H129 (= H134)
binding adenosine monophosphate: R97 (= R102), V99 (≠ P104), R100 (≠ H105), E131 (≠ D136), F145 (≠ Y150), S147 (= S152), V173 (= V181), A177 (= A185)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D225), D213 (= D226), M214 (= M227), D216 (= D229), T217 (= T230), G219 (= G232), T220 (= T233)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
58% identity, 97% coverage: 8:318/321 of query aligns to 4:305/307 of 7xmuA

query
sites
7xmuA

I

L

F

F

A

A

G

G

N

N

A

A

N

T

V

P

T

E

L

L

A

A

E

Q

R

R

I

I

S

A

L

N

Y

R

L

L

D

Y

Q

T

P

S

L

L

G

G

Q

D

A

A

N

A

V

V

G

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

M

S

V

V

Q

Q

I

I

T

N

E

E

S

N

V

V

R

R

G

G

K

G

D

D

V

I

Y

F

V

I

L

I

Q

Q

P

S

T

T

C

C

T

A

P

P

E

T

P

N

A

D

V

-

N

N

L

L

M

M

E

E

M

L

L

V

V

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

K

G

R

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

Y

F

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

P

V

H
x
R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

R

K

L

V

A

V

A

A

D

D

M

F

I

L

T

S

V

S

A

V

G

G

A

V

D

D

R

R

M

V

I

L

T

T

I

V

D

D

L

L

H
|
H

S

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

I

V

Y
x
F

A

G

S
|
S

P

P

V

I

L

L

V

L

E

E

D

D

M

M

L

L

A

Q

Q

L

S

N

G

-

S

-

E

-

V

L

S

D

N

N

I

P

A

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A

A

V

I

A

A

K

K

A

L

L

L

-

N

D

D

A

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

Q

Q

V

V

M

M

N

H

V

I

I

I

G

G

D

D

V

V

R

A

G

G

R

R

D

D

C

C

I

V

I

L

V

V

D
|
D

D

D

M
|
M

V

I

D
|
D

T
|
T

A

G

G

G

T

T

L

L

C

C

K

K

A

A

A

E

A

A

L

L

M

K

E

E

N

R

G

G

A

A

K

K

S

R

V

V

T

F

A

A

Y

Y

A

A

T

T

H

H

A

P

V

I

L

F

S

S

G

G

P

N

A

A

V

A

E

N

N

N

I

L

R

R

K

N

S

S

K

V

L

I

K

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

Q

L

S

S

L

D

D

E

A

I

M

K

E

S

C

L

E

P

K

N

I

V

R

R

Q

T

V

L

S

T

V

L

A

S

N

G

I

M

L

L

G

A

E

E

T

A

I

I

R

R

V

I

N

S

N

N

E

E

S

S

V

I

T

S

A

A

L

M

M

F

binding adenosine-5'-diphosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R99), Q95 (= Q100), R97 (= R102), R97 (= R102), R100 (≠ H105), H129 (= H134), E131 (≠ D136), F145 (≠ Y150), S147 (= S152), V173 (= V181)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D176), D212 (= D225), M214 (= M227), D216 (= D229), T217 (= T230)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
51% identity, 99% coverage: 2:318/321 of query aligns to 6:316/317 of P14193

query
sites
P14193

A

G

N

D

K

K

S

N

V

L

M

K

I

I

F

F

A

S

G

L

N

N

A

S

N

N

V

P

T

E

L

L

A

A

E

K

R

E

I

I

S

A

L

D

Y

I

L

V

D

G

Q

V

P

Q

L

L

G

G

Q

K

A

C

N

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

M

Q

V

I

Q

N

I

I

T

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

Y

Y

V

I

L

I

Q

Q

P

S

T

T

C

S

T

D

P

P

E

-

P

-

A

-

V

V

N

N

-

E

-

H

L

I

M

M

E

E

M

L

L

L

V

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

R

K

P

A

H

R

S

S

A

-

R

R

V

E

P

P

I

I

T

T

A

A

R

K

L

L

A

F

A

A

D

N

M

L

I

L

T

E

V

T

A

A

G

G

A

A

D

T

R

R

M

V

I

I

T

A

I

L

D

D

L

L

H

H

S

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

V

G

E

E

D

Y

M

F

L

-

A

-

Q

-

S

E

G

G

S

K

E

N

V

L

S

E

N

D

I

I

A

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

A

R

L

L

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K
|
K

R

R

R
|
R

P

P

K
x
R

A

P

N
|
N

V

V

S

A

Q
x
E

V

V

M

M

N

N

V

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

V

I

D
|
D

T
|
T

A
|
A

G
|
G

T
|
T

L

I

C

T

K

L

A

A

A

N

A

A

L

L

M

V

E

E

N

N

G

G

A

A

K

K

S

E

V

V

T

Y

A

A

Y

C

A

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

V

V

E

E

N

R

I

I

R

N

K

N

S

S

K

T

L

I

K

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

-

Q

K

S

L

L

P

D

E

A

E

M

K

E

K

C

I

E

E

K

R

I

F

R

K

Q

Q

V

L

S

S

V

V

A

G

N

P

I

L

L

L

G

A

E

E

T

A

I

I

R

I

R

R

V

V

N

H

N

E

E

Q

S

S

V

V

T

S

A

Y

L

L

M

F

RQ 102:103 (= RQ 99:100) binding ATP
K198 (= K199) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R201) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ K203) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N205) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ Q208) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (= DTAGT 229:233) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
49% identity, 98% coverage: 3:318/321 of query aligns to 1:299/299 of 1ibsB

query
sites
1ibsB

N

D

K

K

S

N

V

L

M

K

I

I

F

F

A

S

G

L

N

N

A

S

N

N

V

P

T

E

L

L

A

A

E

K

R

E

I

I

S

A

L

D

Y

I

L

V

D

G

Q

V

P

Q

L

L

G

G

Q

K

A

C

N

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

M

Q

V

I

Q

N

I

I

T

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

Y

Y

V

I

L

I

Q

Q

P

S

T

T

C

S

T

D

P

P

E

-

P

-

A

-

V

V

N

N

-

E

-

H

L

I

M

M

E

E

M

L

L

L

V

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

P

-

H

-

S

-

A

S

R

R

V

E

P

P

I

I

T

T

A

A

R

K

L

L

A

F

A

A

D

N

M

L

I

L

T

E

V

T

A

A

G

G

A

A

D

T

R

R

M

V

I

I

T

A

I

L

D

D

L

L

H

H

S

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

V

G

E

E

D

Y

M

F

L

-

A

-

Q

-

S

E

G

G

S

K

E

N

V

L

S

E

N

D

I

I

A

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

A

R

L

L

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

K

-

A

-

N

-

V

-

S

-

Q

-

V

-

M

M

N

N

V

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

V

I

D

D

T

T

A

A

G

G

T

T

L

I

C

T

K

L

A

A

A

N

A

A

L

L

M

V

E

E

N

N

G

G

A

A

K

K

S

E

V

V

T

Y

A

A

Y

C

A

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

V

V

E

E

N

R

I

I

R

N

K

N

S

S

K

T

L

I

K

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

-

Q

K

S

L

L

P

D

E

A

E

M

K

E

K

C

I

E

E

K

R

I

F

R

K

Q

Q

V

L

S

S

V

V

A

G

N

P

I

L

L

L

G

A

E

E

T

A

I

I

R

I

R

R

V

V

N

H

N

E

E

Q

S

S

V

V

T

S

A

Y

L

L

M

F

binding methyl phosphonic acid adenosine ester: R96 (= R99), Q97 (= Q100), R99 (= R102)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
49% identity, 98% coverage: 5:318/321 of query aligns to 1:295/295 of 1dkuA

query
sites
1dkuA

S

N

V

L

M

K

I

I

F

F

A

S

G

L

N

N

A

S

N

N

V

P

T

E

L

L

A

A

E

K

R

E

I

I

S

A

L

D

Y

I

L

V

D

G

Q

V

P

Q

L

L

G

G

Q

K

A

C

N

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

M

Q

V

I

Q

N

I

I

T

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

Y

Y

V

I

L

I

Q

Q

P

S

T

T

C

S

T

D

P

P

E

V

P

N

A

E

V

-

N

H

L

I

M

M

E

E

M

L

L

L

V

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

F

Y

A

A

R
|
R

Q

Q

D

D

R

R

R
x
K

P

A

H

R

S
|
S

A

-

R
|
R

V

E

P

P

I

I

T

T

A

A

R

K

L

L

A

F

A

A

D

N

M

L

I

L

T

E

V

T

A

A

G

G

A

A

D

T

R

R

M

V

I

I

T

A

I

L

D

D

L

L

H
|
H

S

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D
|
D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

V

G

E

E

D

Y

M

F

L

-

A

-

Q

-

S

E

G

G

S

K

E

N

V

L

S

E

N

D

I

I

A

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

A

R

L

L

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

K

-

A

-

N

-

V

-

S

-

Q

-

V

-

M

M

N

N

V

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

V

I

D

D

T

T

A

A

G

G

T

T

L

I

C

T

K

L

A

A

A

N

A

A

L

L

M

V

E

E

N

N

G

G

A

A

K

K

S

E

V

V

T

Y

A

A

Y

C

A

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

V

V

E

E

N

R

I

I

R

N

K

N

S

S

K

T

L

I

K

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

-

Q

-

S

-

L

-

D

-

A

K

M

L

E

K

C

I

E

E

K

R

I

F

R

K

Q

Q

V

L

S

S

V

V

A

G

N

P

I

L

L

L

G

A

E

E

T

A

I

I

R

I

R

R

V

V

N

H

N

E

E

Q

S
|
S

V
|
V

T
x
S

A

Y

L

L

M
x
F

binding methyl phosphonic acid adenosine ester: R94 (= R99), H128 (= H134)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R103), S101 (= S106), R102 (= R108), D141 (= D147), S290 (= S313), V291 (= V314), S292 (≠ T315), F295 (≠ M318)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
49% identity, 98% coverage: 5:318/321 of query aligns to 1:297/297 of 1ibsA

query
sites
1ibsA

S

N

V

L

M

K

I

I

F

F

A

S

G

L

N

N

A

S

N

N

V

P

T

E

L

L

A

A

E

K

R

E

I

I

S

A

L

D

Y

I

L

V

D

G

Q

V

P

Q

L

L

G

G

Q

K

A

C

N

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

I

V

M

Q

V

I

Q

N

I

I

T

E

E

E

S

S

V

I

R

R

G

G

K

C

D

D

V

C

Y

Y

V

I

L

I

Q

Q

P

S

T

T

C

S

T

D

P

P

E

-

P

-

A

-

V

V

N

N

-

E

-

H

L

I

M

M

E

E

M

L

L

L

V

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

G

G

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

P

-

H

-

S

-

A

S

R

R

V

E

P

P

I

I

T

T

A

A

R

K

L

L

A

F

A

A

D

N

M

L

I

L

T

E

V

T

A

A

G

G

A

A

D

T

R

R

M

V

I

I

T

A

I

L

D

D

L

L

H
|
H

S

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

V

G

E

E

D

Y

M

F

L

-

A

-

Q

-

S

E

G

G

S

K

E

N

V

L

S

E

N

D

I

I

A

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

A

R

L

L

D

K

A

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

K

-

A

-

N

-

V

-

S

-

Q

-

V

-

M

M

N

N

V

I

I

V

G

G

D

N

V

I

R

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

V

I

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

I

C

T

K

L

A

A

A

N

A

A

L

L

M

V

E

E

N

N

G

G

A

A

K

K

S

E

V

V

T

Y

A

A

Y

C

A

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

V

V

E

E

N

R

I

I

R

N

K

N

S

S

K

T

L

I

K

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

-

Q

K

S

L

L

P

D

E

A

E

M

K

E

K

C

I

E

E

K

R

I

F

R

K

Q

Q

V

L

S

S

V

V

A

G

N

P

I

L

L

L

G

A

E

E

T

A

I

I

R

I

R

R

V

V

N

H

N

E

E

Q

S

S

V

V

T

S

A

Y

L

L

M

F

binding methyl phosphonic acid adenosine ester: R94 (= R99), Q95 (= Q100), R97 (= R102), H126 (= H134)
binding sulfate ion: D209 (= D229), T210 (= T230), A211 (= A231), T213 (= T233)

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
47% identity, 98% coverage: 5:318/321 of query aligns to 2:312/316 of 8dbkB

query
sites
8dbkB

S

N

V

I

M

K

I

I

F

F

A

S

G

G

N

S

A

S

N

H

V

Q

T

D

L

L

A

S

E

Q

R

K

I

I

S

A

L

D

Y

R

L

L

D

G

Q

L

P

E

L

L

G

G

Q

K

A

V

N

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

M

C

V

V

Q

E

I

I

T

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

V

V

Y

Y

V

I

L

V

Q

Q

P

S

T

G

C

C

T

G

P

-

E

E

P

I

A

N

V

D

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

R

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

P

D

H

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

A

V

A

A

D

N

M

M

I

L

T

S

V

V

A

A

G

G

A

A

D

D

R

H

M

I

I

I

T

T

I

M

D

D

L

L

H
|
H

S

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

V

L

E

K

D

-

M

W

L

I

A

R

Q

E

S

N

G

I

S

S

E

E

V

W

S

R

N

N

I

C

A

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G
|
G

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

A

R

L

L

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

P

K

K

K

A

A

N
|
N

V

E

S

V

Q

D

V

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D
|
D

M

M

V

A

D
|
D

T
|
T

A
x
C

G
|
G

T
|
T

L

I

C

C

K

H

A

A

A

D

A

K

L

L

M

L

E

S

N

A

G

G

A

A

K

T

S

R

V

V

T

Y

A

A

Y

I

A

L

T

T

H

H

A

G

V

I

L

F

S

S

G

G

P

P

A

A

V

I

E

S

N

R

I

I

R

N

K

N

S

A

K

C

L

F

K

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

Q

Q

S

E

L

D

D

K

A

M

M

K

E

H

C

C

E

S

K

K

I

I

R

Q

Q

V

V

I

S

D

V

I

A

S

N

M

I

I

L

L

G

A

E

E

T

A

I

I

R

R

V

T

N

H

N

N

E

G

E

E

S

S

V

V

T

S

A

Y

L

L

M

F

binding adenosine monophosphate: R95 (= R99), Q96 (= Q100), N199 (= N205)
binding adenosine-5'-triphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41)
binding phosphate ion: S46 (= S49), R48 (= R51)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H134), D170 (= D176), G172 (= G178), K193 (= K199), R195 (= R201), D219 (= D225), D220 (= D226), D223 (= D229), T224 (= T230), C225 (≠ A231), G226 (= G232), T227 (= T233)

8dbeA Human prps1 with adp; hexamer (see paper)
47% identity, 98% coverage: 5:318/321 of query aligns to 2:312/316 of 8dbeA

query
sites
8dbeA

S

N

V

I

M

K

I

I

F

F

A

S

G

G

N

S

A

S

N

H

V

Q

T

D

L

L

A

S

E

Q

R

K

I

I

S

A

L

D

Y

R

L

L

D

G

Q

L

P

E

L

L

G

G

Q

K

A

V

N

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

M

C

V

V

Q

E

I

I

T

G

E

E

S

S

V

V

R

R

G

G

K

E

D

D

V

V

Y

Y

V

I

L

V

Q

Q

P

S

T

G

C

C

T

G

P

-

E

E

P

I

A

N

V

D

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

R

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

P
x
D

H

K

S
|
S

A

-

R
|
R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

A

V

A

A

D

N

M

M

I

L

T

S

V

V

A

A

G

G

A

A

D

D

R

H

M

I

I

I

T

T

I

M

D

D

L

L

H
|
H

S

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

I

L

Y
|
Y

A

A

S

E

P

P

V

A

L

V

V

L

E

K

D

-

M

W

L

I

A

R

Q

E

S

N

G

I

S

S

E

E

V

W

S

R

N

N

I

C

A

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

A

R

L

L

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

K

K

K

A

A

N

N

V

E

S

V

Q

D

V

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D
|
D

M

M

V

A

D
|
D

T
|
T

A

C

G
|
G

T
|
T

L

I

C

C

K

H

A

A

A

D

A

K

L

L

M

L

E

S

N

A

G

G

A

A

K

T

S

R

V

V

T

Y

A

A

Y

I

A

L

T

T

H

H

A

G

V

I

L

F

S

S

G

G

P

P

A

A

V

I

E

S

N

R

I

I

R

N

K

N

S

A

K

C

L

F

K

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

Q

Q

S

E

L

D

D

K

A

M

M

K

E

H

C

C

E

S

K

K

I

I

R

Q

Q

V

V

I

S

D

V

I

A

S

N

M

I

I

L

L

G

A

E

E

T

A

I

I

R

R

V

T

N

H

N

N

E

G

E

E

S
|
S

V
|
V

T
x
S

A

Y

L

L

M
x
F

binding adenosine-5'-diphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), R95 (= R99), Q96 (= Q100), K98 (≠ R102), K99 (≠ R103), D100 (≠ P104), S102 (= S106), R103 (= R108), H129 (= H134), D142 (= D147), Y145 (= Y150), S307 (= S313), V308 (= V314), S309 (≠ T315), F312 (≠ M318)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H134), D170 (= D176), D219 (= D225), D220 (= D226), D223 (= D229), T224 (= T230), G226 (= G232), T227 (= T233)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
47% identity, 98% coverage: 5:318/321 of query aligns to 3:313/318 of P60891

query
sites
P60891

S

N

V

I

M

K

I

I

F

F

A

S

G

G

N

S

A

S

N

H

V

Q

T

D

L

L

A
x
S

E

Q

R

K

I

I

S

A

L

D

Y

R

L

L

D

G

Q

L

P

E

L

L

G

G

Q

K

A

V

N

V

V

T

G

K

R

K

F

F

S

S

D

N

G

Q

E

E

I

T

M

C

V

V

Q

E

I

I

T

G

E

E

S

S

V

V

R

R

G

G

K

E

D
|
D

V

V

Y

Y

V

I

L

V

Q

Q

P

S

T

G

C

C

T

G

P

-

E

E

P

I

A

N

V

D

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

R

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R

R

Q

Q

D

D

R

K

P

D

H

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

A

V

A

A

D
x
N

M

M

I

L

T

S

V

V

A

A

G

G

A

A

D

D

R

H

M

I

I

I

T

T

I

M

D

D

L
|
L

H

H

S

A

D
x
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V
|
V

D

D

N
|
N

I

L

Y
|
Y

A

A

S

E

P

P

V

A

L

V

V

L

E

K

D

-

M

W

L

I

A

R

Q

E

S

N

G

I

S

S

E

E

V

W

S

R

N

N

I

C

A

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K
x
D

A

R

L

L

D

N

A

V

D

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

P

K

K

K

A

A

N

N

V

E

S

V

Q
x
D

V

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

C

A

I

I

I

L

V
|
V

D

D

M

M

V

A

D

D

T

T

A

C

G

G

T

T

L

I

C

C

K
x
H

A

A

A

D

A

K

L

L

M

L

E

S

N

A

G

G

A

A

K

T

S

R

V

V

T

Y

A

A

Y

I

A

L

T

T

H

H

A

G

V

I

L

F

S

S

G

G

P

P

A

A

V

I

E

S

N

R

I

I

R

N

K

N

S

A

K

C

L

F

K

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

Q

Q

S

E

L

D

D

K

A

M

M

K

E

H

C

C

E

S

K

K

I

I

R

Q

Q

V

V

I

S

D

V

I

A

S

N

M

I

I

L

L

G

A

E

E

T

A

I

I

R

R

V

T

N

H

N

N

E

G

E

E

S

S

V

V

T

S

A

Y

L

L

M

F

S16 (≠ A18) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D54) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ D118) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L133) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ D136) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V146) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N148) mutation to H: No effect on catalytic activity.
Y146 (= Y150) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K188) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A195) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D198) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ Q208) to H: in a breast cancer sample; somatic mutation
V219 (= V224) to G: in a breast cancer sample; somatic mutation
H231 (≠ K236) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
46% identity, 99% coverage: 1:318/321 of query aligns to 1:316/321 of O94413

query
sites
O94413

M

M

A

A

N

S

K

N

S

S

V

I

M

K

I

I

F

F

A

A

G

G

N

N

A

S

N

H

V

P

T

E

L

L

A

A

E

E

R

K

I

V

S

A

L

R

Y

R

L

I

D

G

Q

L

P

S

L

L

G

G

Q

K

A

V

N

A

V

V

G

V

R

Q

F

Y

S

S

D

N

G

R

E

E

I

T

M

S

V

V

Q

T

I

I

T

G

E

E

S

S

V

V

R

R

G

D

K

E

D

D

V

V

Y

F

V

I

L

L

Q

Q

P

T

T

G

C

C

T

G

P

-

E

-

P

-

A

S

V

I

N

N

-

D

-

H

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

R

R

S

A

A

S

S

A

A

K

R

R

R

I

I

T

T

A

A

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R

R

Q

Q

D

D

R

K

P

D

H

K

S

S

A

-

R

R

V

A

P

P

I

I

T

T

A

A

R

R

L

L

A

V

A

A

D

N

M

M

I

L

T

Q

V

T

A

A

G

G

A

C

D

N

R

H

M

I

I

I

T

T

I

M

D

D

L

L

H

H

S

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

N

I

V

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

S

L

V

V

L

E

R

D

Y

M

I

L

R

A

E

Q

N

S

I

G

D

S

T

E

T

V

V

S

N

N

P

I

T

A

V

V

I

V

V

S
|
S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

A

R

T

A

A

V

L

A

A

K

D

A

R

L

L

D

D

A

L

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

Q

K

K

A

A

N

N

V

E

S

V

Q

S

V

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

R

G

D

R

K

D

L

C

A

I

I

I

L

V

V

D

D

M

M

V

A

D

D

T

T

A

C

G

G

T

T

L

L

C

G

K

L

A

A

A

K

A

T

L

L

M

K

E

D

N

N

G

G

A

A

K

K

S

A

V

V

T

Y

A

A

Y

I

A

V

T

T

H

H

A

G

V

I

L

L

S

S

G

G

P

K

A

A

V

I

E

K

N

V

I

I

R

N

K

E

S

S

K

A

L

L

K

E

E

K

L

V

V

I

V

V

T

T

D

N

T

T

I

I

P

P

Q

H

S

D

L

D

D

K

A

R

M

S

E

L

C

C

E

S

K

K

I

I

R

E

Q

T

V

I

S

D

V

I

A

S

N

G

I

V

L

L

G

A

E

E

T

C

I

I

R

R

V

I

N

H

E

G

E

E

S

S

V

V

T

S

A

V

L

L

M

F

S172 (= S174) modified: Phosphoserine

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
46% identity, 98% coverage: 5:318/321 of query aligns to 1:305/305 of 2hcrA

query
sites
2hcrA

S

N

V

I

M

K

I

I

F

F

A

S

G

G

N

S

A

S

N

H

V

Q

T

D

L

L

A

S

E

Q

R

K

I

I

S

A

L

D

Y

R

L

L

D

G

Q

L

P

E

L

L

G

G

Q

K

A

V

N

V

V

T

G

K

R

K

F

F

S

S

D

N

G

Q

E

E

I

T

M

C

V

V

Q

E

I

I

T

G

E

E

S

S

V

V

R

R

G

G

K

E

D

D

V

V

Y

Y

V

I

L

V

Q

Q

P

S

T

G

C

C

T

G

P

-

E

E

P

I

A

N

V

D

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

R

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

P

D

H

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

A

V

A

A

D

N

M

M

I

L

T

S

V

V

A

A

G

G

A

A

D

D

R

H

M

I

I

I

T

T

I

M

D

D

L

L

H
|
H

S

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

V

L

E

K

D

-

M

W

L

I

A

R

Q

E

S

N

G

I

S

S

E

E

V

W

S

R

N

N

I

C

A

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

A

R

L

L

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

D

K

R

A

-

N

-

V

-

S

-

Q

-

V

-

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D

D

M

M

V

A

D

D

T

T

A

C

G

G

T

T

L

I

C

C

K

H

A

A

A

D

A

K

L

L

M

L

E

S

N

A

G

G

A

A

K

T

S

R

V

V

T

Y

A

A

Y

I

A

L

T

T

H

H

A

G

V

I

L

F

S

S

G

G

P

P

A

A

V

I

E

S

N

R

I

I

R

N

K

N

S

A

K

C

L

F

K

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

Q

Q

S

E

L

D

D

K

A

M

M

K

E

H

C

C

E

S

K

K

I

I

R

Q

Q

V

V

I

S

D

V

I

A

S

N

M

I

I

L

L

G

A

E

E

T

A

I

I

R

R

V

T

N

H

N

N

E

G

E

E

S

S

V

V

T

S

A

Y

L

L

M

F

binding adenosine monophosphate: R94 (= R99), Q95 (= Q100), K97 (≠ R102), H128 (= H134)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
46% identity, 98% coverage: 5:318/321 of query aligns to 2:305/309 of 8dbgA

query
sites
8dbgA

S

N

V

I

M

K

I

I

F

F

A

S

G

G

N

S

A

S

N

H

V

Q

T

D

L

L

A

S

E

Q

R

K

I

I

S

A

L

D

Y

R

L

L

D

G

Q

L

P

E

L

L

G

G

Q

K

A

V

N

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

M

C

V

V

Q

E

I

I

T

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

V

V

Y

Y

V

I

L

V

Q

Q

P

S

T

G

C

C

T

G

P

-

E

E

P

I

A

N

V

D

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

R

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

P

D

H

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

A

V

A

A

D

N

M

M

I

L

T

S

V

V

A

A

G

G

A

A

D

D

R

H

M

I

I

I

T

T

I

M

D

D

L

L

H
|
H

S

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

V

L

E

K

D

-

M

W

L

I

A

R

Q

E

S

N

G

I

S

S

E

E

V

W

S

R

N

N

I

C

A

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

A

R

L

L

D

N

A

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

K

-

A

-

N

-

V

-

S

-

Q

D

V

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D

D

M

M

V

A

D
|
D

T
|
T

A
x
C

G

G

T
|
T

L

I

C

C

K

H

A

A

A

D

A

K

L

L

M

L

E

S

N

A

G

G

A

A

K

T

S

R

V

V

T

Y

A

A

Y

I

A

L

T

T

H

H

A

G

V

I

L

F

S

S

G

G

P

P

A

A

V

I

E

S

N

R

I

I

R

N

K

N

S

A

K

C

L

F

K

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

Q

Q

S

E

L

D

D

K

A

M

M

K

E

H

C

C

E

S

K

K

I

I

R

Q

Q

V

V

I

S

D

V

I

A

S

N

M

I

I

L

L

G

A

E

E

T

A

I

I

R

R

V

T

N

H

N

N

E

G

E

E

S

S

V

V

T

S

A

Y

L

L

M

F

binding adenosine-5'-triphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), R95 (= R99), Q96 (= Q100), K98 (≠ R102), H129 (= H134)
binding phosphate ion: S46 (= S49), R48 (= R51), D216 (= D229), T217 (= T230), C218 (≠ A231), T220 (= T233)

7yk1A Structural basis of human prps2 filaments (see paper)
45% identity, 98% coverage: 5:318/321 of query aligns to 2:303/306 of 7yk1A

query
sites
7yk1A

S

N

V

I

M

V

I

L

F

F

A

S

G

G

N

S

A

S

N

H

V

Q

T

D

L

L

A

S

E

Q

R

R

I

V

S

A

L

D

Y

R

L

L

D

G

Q

L

P

E

L

L

G

G

Q

K

A

V

N

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

M

S

V

V

Q

E

I

I

T

G

E

E

S
|
S

V

V

R
|
R

G

G

K

E

D

D

V

V

Y

Y

V

I

L

I

Q

Q

P

S

T

G

C

C

T

G

P

-

E

E

P

I

A

N

V

D

N

N

L

L

M

M

E

E

M

L

L

L

V

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

S

K

S

R

R

I

V

T

T

A

A

V

V

V

I

P

P

Y

C

Y

F

G

P

F

Y

A

A

R
|
R

Q

Q

D

D

R

K

R
x
K

P
x
D

H
x
K

S
|
S

A

-

R
|
R

V

A

P

P

I

I

T

S

A

A

R

K

L

L

A

V

A

A

D

N

M

M

I

L

T

S

V

V

A

A

G

G

A

A

D

D

R

H

M

I

I

I

T

T

I

M

D

D

L

L

H
|
H

S

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

V

L

E

Q

D

-

M

W

L

I

A

R

Q

E

S

N

G

I

S

A

E

E

V

W

S

K

N

N

I

C

A

I

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

A

R

L

L

D

N

A

V

D

E

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

K

-

A

-

N

-

V

-

S

-

Q

-

V

-

M

M

N

V

V

L

I

V

G

G

D

D

V

V

R

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D

D

M

M

V

A

D
|
D

T

T

A
x
C

G

G

T
|
T

L

I

C

C

K

H

A

A

A

D

A

K

L

L

M

L

E

S

N

A

G

G

A

A

K

T

S

K

V

V

T

Y

A

A

Y

I

A

L

T

T

H

H

A

G

V

I

L

F

S

S

G

G

P

P

A

A

V

I

E

S

N

R

I

I

R

N

K

N

S

A

K

A

L

F

K

E

E

A

L

V

V

V

T

T

D

N

T

T

I

I

P

P

Q

Q

S

E

L

D

D

K

A

M

M

K

E

H

C

C

E

T

K

K

I

I

R

Q

Q

V

V

I

S

D

V

I

A

S

N

M

I

I

L

L

G

A

E

E

T

A

I

I

R

R

V

T

N

H

N

N

E

G

E

E

S
|
S

V

V

T
x
S

A

Y

L

L

M
x
F

binding adenosine-5'-diphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), S46 (= S49), R48 (= R51), R95 (= R99), K99 (≠ R103), D100 (≠ P104), K101 (≠ H105), S102 (= S106), R103 (= R108), H129 (= H134), D142 (= D147), S298 (= S313), S300 (≠ T315), F303 (≠ M318)
binding phosphate ion: D214 (= D229), C216 (≠ A231), T218 (= T233)

Query Sequence

>WP_013835916.1 NCBI__GCF_000214825.1:WP_013835916.1
MANKSVMIFAGNANVTLAERISLYLDQPLGQANVGRFSDGEIMVQITESVRGKDVYVLQP
TCTPEPAVNLMEMLVMIDALKRASAKRITAVVPYYGFARQDRRPHSARVPITARLAADMI
TVAGADRMITIDLHSDQIQGFFDIPVDNIYASPVLVEDMLAQSGSEVSNIAVVSPDVGGV
VRARAVAKALDADLAIIDKRRPKANVSQVMNVIGDVRGRDCIIVDDMVDTAGTLCKAAAA
LMENGAKSVTAYATHAVLSGPAVENIRKSKLKELVVTDTIPQSLDAMECEKIRQVSVANI
LGETIRRVNNEESVTALMPAM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory