SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013840109.1 NCBI__GCF_000215085.1:WP_013840109.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 96% coverage: 5:375/385 of query aligns to 9:386/401 of Q56YA5

query
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Q56YA5

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TGT 68:70 (≠ SGT 63:65) binding pyridoxal 5'-phosphate
T148 (= T141) binding pyridoxal 5'-phosphate
QK 200:201 (= QK 191:192) binding pyridoxal 5'-phosphate
K201 (= K192) binding 3-hydroxypyruvate
P251 (≠ T241) mutation to L: Abolishes aminotransferase activity.
R347 (= R336) binding 3-hydroxypyruvate

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
36% identity, 96% coverage: 5:375/385 of query aligns to 8:385/400 of 6pk3B

query
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binding pyridoxal-5'-phosphate: T67 (≠ S63), G68 (= G64), T69 (= T65), W72 (≠ L68), F94 (= F90), T147 (= T141), D174 (= D166), V176 (= V168), Q199 (= Q191), K200 (= K192)

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
36% identity, 96% coverage: 5:375/385 of query aligns to 7:384/399 of 6pk1A

query
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6pk1A

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binding 3-hydroxypyruvic acid: F93 (= F90), R345 (= R336)
binding pyridoxal-5'-phosphate: T66 (≠ S63), G67 (= G64), T68 (= T65), W71 (≠ L68), F93 (= F90), T146 (= T141), D173 (= D166), V175 (= V168), K199 (= K192)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
31% identity, 97% coverage: 6:379/385 of query aligns to 8:385/387 of 3islA

query
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3islA

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R

E

I

R

Y

Y

G

G

G

A

N

N

V

V

D

H

F

M

V

L

D

E

F

C

G

E

W

W

G

G

Y

T

D

V

I

F

D

D

L

P

N

E

V

D

V

I

K

I

R

R

K

E

L

I

D

K

E

K

D

V

S

K

A

P

I

-

K

K

V

I

V

V

L

A

A

M

T

V

Q

H

N

G

E

E

T
|
T

S

S

T

T

A

G

V

R

Q

I

N

H

D

P

I

L

E

K

G

A

L

I

G

G

K

E

L

A

V

C

A

R

Q

T

Y

E

D

D

A

A

V

L

L

F

A

I

V

V

D
|
D

A

A

V
|
V

S

A

G

T

L

I

A

G

I

C

D

E

L

V

K

K

T

V

D

D

E

E

W

W

N

K

V

I

D

D

M

A

V

A

V

I

S

G

G

G

S

T

Q
|
Q

K
|
K

A

C

F

L

M

S

L

V

P

P

P

S

G

G

L

M

A

A

F

P

I

I

S

T

V

Y

S

N

D

E

K

R

A

V

W

A

A

D

K

V

I

I

E

A

K

A

N

R

T

N

S

R

P

P

V

I

Y

T

-

S

-

N

Y

Y

F

F

D

D

L

L

K

S

K

Q

A

L

K

E

K

D

S

Y

I

W

A

S

K

E

W

R

N

R

-

L

T

N

A

H

Y

H

T

T

T

E

P

A

V

T

S

T

M

M

V

L

Y

Y

G

A

L

L

E

R

A

E

A

G

L

V

D

R

M

L

I

V

L

L

E

E

G

G

L

L

P

E

Q

T

V

R

F

F

A

E

R

R

H

H

A

R

L

H

A

E

K

A

A

A

T

L

R

A

A

A

A

G

V

I

Q

K

G

A

L

M

G

G

L

L

E

R

L

L

L

F

A

G

P

D

D

D

E

S

C

C

A

K

S

M

K

P

A

V

V

V

T

T

A

C

V

V

Q

E

S

I

P

P

M

G

V

G

V

I

D

D

A

G

D

E

T

S

L

V

R

R

K

D

V

M

L

L

K

A

E

Q

Y

F

G

G

V

I

T

E

F

I

A

A

G

S

Q

F

E

G

M

P

M

L

K

A

G

G

K

K

I

I

F

W

R

R

I

I

A

G

H

T

M

M

G

G

Y

Y

A

S

G

C

-

R

K

K

M

E

D

N

V

V

I

L

I

F

A

V

I

L

A

A

A

G

L

L

E

E

M

A

A

V

L

L

G

L

K

R

C

H

G

N

Y

A

K

G

A

I

E

E

L

A

G

G

A

K

G

A

V

L

R

Q

E

A

A

A

Q

L

M

D

V

V

F

Y

binding pyridoxal-5'-phosphate: T66 (≠ S63), S67 (≠ G64), R68 (≠ T65), F93 (= F90), T143 (= T141), D168 (= D166), V170 (= V168), Q193 (= Q191), K194 (= K192)

2z9xA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxyl-l-alanine (see paper)
34% identity, 98% coverage: 4:379/385 of query aligns to 8:388/392 of 2z9xA

query
sites
2z9xA

D

D

K

P

K

V

Y

I

L

T

L

L

I

T

P

A

G

G

P

P

T

V

P

N

V

A

P

Y

P

P

R

E

V

V

V

L

E

R

A

G

M

L

S

G

R

R

P

T

I

V

I

L

G

Y

H

D

R

Y

S

D

A

P

G

A

F

F

Q

Q

A

L

V

L

M

Y

E

E

R

K

V

V

T

V

G

D

K

K

L

A

K

Q

K

K

V

A

F

M

Q

R

T

L

E

S

N

N

H

K

V

P

F

V

I

I

L

L

G

H

S

G

S
x
E

G

P

T

V

G

L

A

G

L

L

E

E

A

A

V

A

A

A

N

S

L

L

V

I

H

S

P

P

G

D

D

D

K

V

V

V

L

L

A

N

L

L

S

A

C

S

G

G

K

V

F
x
Y

G

G

E

K

R

G

F

F

A

G

E

Y

L

W

A

A

R

K

I

R

Y

Y

G

S

G

P

N

H

V

L

D

L

F

E

V

I

D

E

F

V

G

P

W

Y

G

N

Y

E

D

A

I

I

D

D

L

P

N

Q

V

A

V

V

K

A

R

D

K

M

L

L

D

K

E

A

D

H

S

P

A

E

I

I

K

T

V

V

L

S

A

V

T

C

Q

H

N

H

E

D

T
|
T

S

P

T

S

A

G

V

T

Q

I

N

N

D

P

I

I

E

D

G

A

L

I

G

G

K

A

L

L

V

V

A

S

Q

A

Y

H

D

G

A

A

V

Y

L

L

A

I

V

V

D
|
D

A

A

V
|
V

S

S

G

S

L

F

A

G

I

M

D

K

L

T

K

H

T

P

D

E

E

D

W

C

N

K

V

A

D

D

M

I

V

Y

V

V

S

T

G

G

S

P

Q

N

K
|
K

A

C

F

L

M

G

L

A

P

P

G

G

L

L

A

T

F

M

I

M

S

G

V

V

S

S

D

E

K

R

A

A

W

W

A

A

K

K

I

M

E

K

K

A

N

N

T

P

S

-

P

-

V

-

Y

-

F

L

D

A

L

P

K

R

K

A

A

S

K

M

K

L

S

S

I

I

A

V

K

D

W

W

N

E

T

N

A

A

Y

W

T

S

-

R

-

D

-

K

-

P

-

F

-

P

-

F

T

T

P

P

-

S

V

V

S

S

M

E

V

I

Y

N

G

G

L

L

E

D

A

V

A

A

L

L

D

D

M

L

I

Y

L

L

E

N

E

E

G

G

L

P

P

E

Q

A

V

V

F

W

A

A

R

R

H

H

A

A

L

L

L

T

A

A

K

K

A

A

T

M

R

R

A

A

A

G

V

V

Q

T

G

A

L

M

G

G

L

L

E

S

L

V

L

W

-

A

A

A

P

S

D

D

E

S

C

I

A

A

S

S

K

P

A

T

V

T

T

T

A

A

V

V

Q

R

S

T

P

P

M

D

V

G

V

V

D

D

A

E

D

K

T

A

L

L

R

R

K

Q

V

A

L

A

L

R

K

A

E

R

Y

Y

G

G

V

V

T

V

F

F

A

S

G

S

G

G

Q

R

E

G

M

E

M

T

K

L

G

G

K

K

I

L

F

T

R
|
R

I

I

A

G

H

H

M

M

G

G

-

P

Y

T

A

A

G

Q

K

P

M

I

D

Y

V

A

I

I

I

A

A

A

I

L

A

T

A

A

L

L

E

G

M

G

A

A

L

M

G

N

K

A

C

A

G

G

Y

R

K

K

A

L

E

A

L

I

G

G

A

K

G

G

V

I

R

E

E

A

A

A

Q

L

M

A

V

V

F

I

binding alanine: Y94 (≠ F90), R344 (= R336)
binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ S63), Y94 (≠ F90), T145 (= T141), D170 (= D166), V172 (= V168), K196 (= K192)

2z9wA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxal (see paper)
34% identity, 98% coverage: 4:379/385 of query aligns to 8:388/392 of 2z9wA

query
sites
2z9wA

D

D

K

P

K

V

Y

I

L

T

L

L

I

T

P

A

G

G

P

P

T

V

P

N

V

A

P

Y

P

P

R

E

V

V

V

L

E

R

A

G

M

L

S

G

R

R

P

T

I

V

I

L

G

Y

H

D

R

Y

S

D

A

P

G

A

F

F

Q

Q

A

L

V

L

M

Y

E

E

R

K

V

V

T

V

G

D

K

K

L

A

K

Q

K

K

V

A

F

M

Q

R

T

L

E

S

N

N

H

K

V

P

F

V

I

I

L

L

G

H

S

G

S
x
E

G
x
P

T

V

G

L

A

G

L

L

E

E

A

A

V

A

A

A

N

S

L

L

V

I

H

S

P

P

G

D

D

D

K

V

V

V

L

L

A

N

L

L

S

A

C

S

G

G

K

V

F
x
Y

G

G

E

K

R

G

F

F

A

G

E

Y

L

W

A

A

R

K

I

R

Y

Y

G

S

G

P

N

H

V

L

D

L

F

E

V

I

D

E

F

V

G

P

W

Y

G

N

Y

E

D

A

I

I

D

D

L

P

N

Q

V

A

V

V

K

A

R

D

K

M

L

L

D

K

E

A

D

H

S

P

A

E

I

I

K

T

V

V

L

S

A

V

T

C

Q

H

N

H

E

D

T
|
T

S

P

T

S

A

G

V

T

Q

I

N

N

D

P

I

I

E

D

G

A

L

I

G

G

K

A

L

L

V

V

A

S

Q

A

Y

H

D

G

A

A

V

Y

L

L

A

I

V

V

D
|
D

A

A

V
|
V

S
|
S

G

S

L

F

A

G

I

M

D

K

L

T

K

H

T

P

D

E

E

D

W

C

N

K

V

A

D

D

M

I

V

Y

V

V

S

T

G

G

S

P

Q

N

K
|
K

A

C

F

L

M

G

L

A

P

P

G

G

L

L

A

T

F

M

I

M

S

G

V

V

S

S

D

E

K

R

A

A

W

W

A

A

K

K

I

M

E

K

K

A

N

N

T

P

S

-

P

-

V

-

Y

-

F

L

D

A

L

P

K

R

K

A

A

S

K

M

K

L

S

S

I

I

A

V

K

D

W

W

N

E

T

N

A

A

Y

W

T

S

-

R

-

D

-

K

-

P

-

F

-

P

-

F

T

T

P

P

-

S

V

V

S

S

M

E

V

I

Y

N

G

G

L

L

E

D

A

V

A

A

L

L

D

D

M

L

I

Y

L

L

E

N

E

E

G

G

L

P

P

E

Q

A

V

V

F

W

A

A

R

R

H

H

A

A

L

L

L

T

A

A

K

K

A

A

T

M

R

R

A

A

A

G

V

V

Q

T

G

A

L

M

G

G

L

L

E

S

L

V

L

W

-

A

A

A

P

S

D

D

E

S

C

I

A

A

S

S

K

P

A

T

V

T

T

T

A

A

V

V

Q

R

S

T

P

P

M

D

V

G

V

V

D

D

A

E

D

K

T

A

L

L

R

R

K

Q

V

A

L

A

L

R

K

A

E

R

Y

Y

G

G

V

V

T

V

F

F

A

S

G

S

G

G

Q

R

E

G

M

E

M

T

K

L

G

G

K

K

I

L

F

T

R

R

I

I

A

G

H

H

M

M

G

G

-

P

Y

T

A

A

G

Q

K

P

M

I

D

Y

V

A

I

I

I

A

A

A

I

L

A

T

A

A

L

L

E

G

M

G

A

A

L

M

G

N

K

A

C

A

G

G

Y

R

K

K

A

L

E

A

L

I

G

G

A

K

G

G

V

I

R

E

E

A

A

A

Q

L

M

A

V

V

F

I

binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ S63), P68 (≠ G64), Y94 (≠ F90), T145 (= T141), D170 (= D166), V172 (= V168), S173 (= S169), K196 (= K192)

2z9vA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine (see paper)
34% identity, 98% coverage: 4:379/385 of query aligns to 8:388/392 of 2z9vA

query
sites
2z9vA

D

D

K

P

K

V

Y

I

L

T

L

L

I

T

P

A

G

G

P

P

T

V

P

N

V

A

P

Y

P

P

R

E

V

V

V

L

E

R

A

G

M

L

S

G

R

R

P

T

I

V

I

L

G

Y

H

D

R

Y

S

D

A

P

G

A

F

F

Q

Q

A

L

V

L

M

Y

E

E

R

K

V

V

T

V

G

D

K

K

L

A

K

Q

K

K

V

A

F

M

Q

R

T

L

E

S

N

N

H

K

V

P

F

V

I

I

L

L

G

H

S

G

S
x
E

G

P

T
x
V

G

L

A

G

L

L

E

E

A

A

V

A

A

A

N

S

L

L

V

I

H

S

P

P

G

D

D

D

K

V

V

V

L

L

A

N

L

L

S

A

C

S

G

G

K

V

F
x
Y

G

G

E

K

R

G

F

F

A

G

E

Y

L

W

A

A

R

K

I

R

Y

Y

G

S

G

P

N

H

V

L

D

L

F

E

V

I

D

E

F

V

G

P

W

Y

G

N

Y

E

D

A

I

I

D

D

L

P

N

Q

V

A

V

V

K

A

R

D

K

M

L

L

D

K

E

A

D

H

S

P

A

E

I

I

K

T

V

V

L

S

A

V

T

C

Q

H

N

H

E

D

T
|
T

S

P

T

S

A

G

V

T

Q

I

N

N

D

P

I

I

E

D

G

A

L

I

G

G

K

A

L

L

V

V

A

S

Q

A

Y

H

D

G

A

A

V

Y

L

L

A

I

V

V

D
|
D

A

A

V
|
V

S

S

G

S

L

F

A

G

I

M

D

K

L

T

K

H

T

P

D

E

E

D

W

C

N

K

V

A

D

D

M

I

V

Y

V

V

S

T

G

G

S

P

Q

N

K

K

A

C

F

L

M

G

L

A

P

P

G

G

L

L

A

T

F

M

I

M

S

G

V

V

S

S

D

E

K

R

A

A

W

W

A

A

K

K

I

M

E

K

K

A

N

N

T

P

S

-

P

-

V

-

Y

-

F

L

D

A

L

P

K

R

K

A

A

S

K

M

K

L

S

S

I

I

A

V

K

D

W

W

N

E

T

N

A

A

Y

W

T

S

-

R

-

D

-

K

-

P

-

F

-

P

-

F

T

T

P

P

-

S

V

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S

S

M

E

V

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Y

N

G

G

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L

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L

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M

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A

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H

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K

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T

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M

G

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-

A

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D

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T

A

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A

L

A

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R

K

A

E

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Y

Y

G

G

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F

F

A

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L

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I

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I

I

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M

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-

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Y

T

A

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D

Y

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A

I

I

I

A

A

A

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A

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A

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I

binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: E67 (≠ S63), V69 (≠ T65), Y94 (≠ F90), T145 (= T141), D170 (= D166), V172 (= V168)

Q988B8 Pyridoxamine--pyruvate transaminase; Pyridoxamine-pyruvate aminotransferase; EC 2.6.1.30 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
34% identity, 98% coverage: 4:379/385 of query aligns to 9:389/393 of Q988B8

query
sites
Q988B8

D

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x
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-

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x
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|
R

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I

E68 (≠ S63) binding pyridoxal 5'-phosphate; mutation E->A,G: Low but detectable pyridoxamine--pyruvate transaminase activity.
Y95 (≠ F90) binding pyridoxal 5'-phosphate
T146 (= T141) binding pyridoxal 5'-phosphate
K197 (= K192) modified: N6-(pyridoxal phosphate)lysine; mutation to L: Loss of function.
C198 (≠ A193) mutation to A: No effect on enzyme activity.
R336 (≠ Q327) mutation to A: Strongly decreased affinity for pyruvate.
R345 (= R336) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
30% identity, 97% coverage: 6:379/385 of query aligns to 12:407/416 of O32148

query
sites
O32148

K

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x
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x
N

A

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H

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A

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F

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A

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L

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A

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E

A

A

A

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D

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Y

Q37 (≠ H31) mutation to H: 5-fold decrease in transamination activity.
K198 (= K192) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ T237) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
33% identity, 89% coverage: 1:341/385 of query aligns to 15:361/385 of 2huuA

query
sites
2huuA

M

L

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Y

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P

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K

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Y

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L

F

F

Q

Q

T

T

E

N

N

N

-

I

H

A

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F

F

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C

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L

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A

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S

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H

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A

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E

E

A

A

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C

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N

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x
S

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K

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x
Y

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M

T

K

A

G

G

K

Q

I

V

F

F

R
|
R

I

I

A

G

H

L

M

M

G

G

binding 1-butanol: H45 (= H31), Y257 (vs. gap)
binding alanine: P25 (= P11), S155 (≠ T141), K206 (= K192), L347 (≠ Q327), R356 (= R336)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
33% identity, 89% coverage: 1:341/385 of query aligns to 15:361/385 of 2huiA

query
sites
2huiA

M

L

M

V

K

T

D

P

K

N

K

K

Y

L

L

L

L

M

I

G

P
|
P

G

G

P

P

T

S

P

N

V

A

P

P

P

Q

R

R

V

V

V

L

E

D

A

A

M

M

S

S

R

R

P

P

I

I

I

L

G

G

H

H

R

L

S

H

A

P

G

E

F

T

Q

L

A

K

V

I

M

M

E

D

R

D

V

I

T

K

G

E

K

G

L

V

K

R

K

Y

V

L

F

F

Q

Q

T

T

E

N

N

N

-

I

H

A

V

T

F

F

I

C

L

L

G

S

S

A

S

S

G

G

T

H

G

G

A

G

L

M

E

E

A

A

A

T

V

L

A

C

N

N

L

L

V

L

H

E

P

D

G

G

D

D

K

V

V

I

L

L

A

I

L

G

S

H

C

T

G

G

K

H

F

W

G

G

E

D

R

R

F

S

A

A

E

D

L

M

A

A

R

T

I

R

Y

Y

G

G

G

A

N

D

V

V

D

R

F

V

V

V

D

K

F

S

G

K

W

V

G

G

Y

Q

D

S

I

L

D

S

L

L

N

D

V

E

V

I

K

R

R

D

K

A

L

L

D

L

E

I

D

H

S

K

A

P

I

-

K

S

V

V

V

L

L

F

A

L

T

T

Q

Q

N

G

E

D

T
x
S

S

S

T

T

A

G

V

V

Q

L

N

Q

D

G

I

L

E

E

G

G

L

V

G

G

K

A

L

L

V

C

A

H

Q

Q

Y

H

D

N

A

C

V

L

L

L

A

I

V

V

D

D

A

T

V

V

S

A

G

S

L

L

A

G

I

A

D

P

L

M

K

F

T

M

D

D

E

R

W

W

N

E

V

I

D

D

M

A

V

M

V

Y

S

T

G

G

S

S

Q

Q

K
|
K

A

V

F

L

M

G

L

A

P

P

G

G

L

I

A

T

F

P

I

V

S

S

V

F

S

S

D

H

K

R

A

A

W

V

A

E

K

R

I

Y

E

K

K

R

N

R

T

N

S

T

-

K

-

V

P

K

V

V

Y

Y

F

W

D

D

L

M

K

S

K

L

A

V

K

G

K

D

S

Y

I

W

A

G

K

C

W

F

N

G

T

R

A

P

-

R

-

I

-

Y

-

H

Y

H

T

T

T

I

P

S

V

S

S

T

M

L

V

L

Y

Y

G

G

L

L

E

R

A

E

A

A

L

I

D

A

M

M

I

A

L

C

E

E

G

G

L

L

P

P

Q

A

V

L

F

I

A

A

R

R

H

H

A

E

L

D

L

C

A

A

K

K

A

R

T

L

R

Y

A

R

A

G

V

L

Q

Q

G

D

L

A

G

G

L

F

E

E

L

L

L

Y

A

A

P

D

D

P

E

K

C

D

A

R

S

L

K

S

A

T

V

V

T

T

A

T

V

I

Q

K

S

V

P

P

M

Q

V

G

V

V

D

D

A

W

D

L

T

K

L

A

R

A

K

Q

V

Y

L

A

L

M

K

K

E

T

Y

Y

G

L

V

V

T

E

F

I

A

S

G

G

Q

L

E

G

M

P

M

T

K

A

G

G

K

Q

I

V

F

F

R
|
R

I

I

A

G

H

L

M

M

G

G

binding glyoxylic acid: P25 (= P11), S155 (≠ T141), K206 (= K192), R356 (= R336)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
33% identity, 89% coverage: 1:341/385 of query aligns to 15:361/385 of 2hufA

query
sites
2hufA

M

L

M

V

K

T

D

P

K

N

K

K

Y

L

L

L

L

M

I

G

P

P

G

G

P

P

T

S

P

N

V

A

P

P

P

Q

R

R

V

V

V

L

E

D

A

A

M

M

S

S

R

R

P

P

I

I

I

L

G

G

H
|
H

R

L

S

H

A

P

G

E

F

T

Q

L

A

K

V

I

M

M

E

D

R

D

V

I

T

K

G

E

K

G

L

V

K

R

K

Y

V

L

F

F

Q

Q

T

T

E

N

N

N

-

I

H

A

V

T

F

F

I

C

L

L

G

S

S

A

S

S

G

G

T

H

G

G

A

G

L

M

E

E

A

A

A

T

V

L

A

C

N

N

L

L

V

L

H

E

P

D

G

G

D

D

K

V

V

I

L

L

A

I

L

G

S

H

C

T

G

G

K

H

F

W

G

G

E

D

R

R

F

S

A

A

E

D

L

M

A

A

R

T

I

R

Y

Y

G

G

G

A

N

D

V

V

D

R

F

V

V

V

D

K

F

S

G

K

W

V

G

G

Y

Q

D

S

I

L

D

S

L

L

N

D

V

E

V

I

K

R

R

D

K

A

L

L

D

L

E

I

D

H

S

K

A

P

I

-

K

S

V

V

V

L

L

F

A

L

T

T

Q

Q

N

G

E

D

T
x
S

S

S

T

T

A

G

V

V

Q

L

N

Q

D

G

I

L

E

E

G

G

L

V

G

G

K

A

L

L

V

C

A

H

Q

Q

Y

H

D

N

A

C

V

L

L

L

A

I

V

V

D

D

A

T

V

V

S

A

G

S

L

L

A

G

I

A

D

P

L

M

K

F

T

M

D

D

E

R

W

W

N

E

V

I

D

D

M

A

V

M

V

Y

S

T

G

G

S

S

Q

Q

K

K

A

V

F

L

M

G

L

A

P

P

G

G

L

I

A

T

F

P

I

V

S

S

V

F

S

S

D

H

K

R

A

A

W

V

A

E

K

R

I

Y

E

K

K

R

N

R

T

N

S

T

-

K

-

V

P

K

V

V

Y

Y

F

W

D

D

L

M

K

S

K

L

A

V

K

G

K

D

S

Y

I

W

A

G

K

C

W

F

N

G

T

R

A

P

-

R

-

I

-
x
Y

-

H

Y

H

T

T

T

I

P

S

V

S

S

T

M

L

V

L

Y

Y

G

G

L

L

E

R

A

E

A

A

L

I

D

A

M

M

I

A

L

C

E

E

G

G

L

L

P

P

Q

A

V

L

F

I

A

A

R

R

H

H

A

E

L

D

L

C

A

A

K

K

A

R

T

L

R

Y

A

R

A

G

V

L

Q

Q

G

D

L

A

G

G

L

F

E

E

L

L

L

Y

A

A

P

D

D

P

E

K

C

D

A

R

S

L

K

S

A

T

V

V

T

T

A

T

V

I

Q

K

S

V

P

P

M

Q

V

G

V

V

D

D

A

W

D

L

T

K

L

A

R

A

K

Q

V

Y

L

A

L

M

K

K

E

T

Y

Y

G

L

V

V

T

E

F

I

A

S

G

G

Q

L

E

G

M

P

M

T

K

A

G

G

K

Q

I

V

F

F

R
|
R

I

I

A

G

H

L

M

M

G

G

binding 1-butanol: H45 (= H31), S155 (≠ T141), Y257 (vs. gap), R356 (= R336)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
33% identity, 89% coverage: 1:341/385 of query aligns to 15:361/393 of Q3LSM4

query
sites
Q3LSM4

M

L

M

V

K

T

D

P

K

N

K

K

Y

L

L

L

L

M

I

G

P

P

G

G

P

P

T

S

P

N

V

A

P

P

P

Q

R

R

V

V

V

L

E

D

A

A

M

M

S

S

R

R

P

P

I

I

I

L

G

G

H

H

R

L

S

H

A

P

G

E

F

T

Q

L

A

K

V

I

M

M

E

D

R

D

V

I

T

K

G

E

K

G

L

V

K

R

K

Y

V

L

F

F

Q

Q

T

T

E

N

N

N

-

I

H

A

V

T

F

F

I

C

L

L

G

S

S

A

S
|
S

G
|
G

T
x
H

G

G

A

G

L

M

E

E

A

A

A

T

V

L

A

C

N

N

L

L

V

L

H

E

P

D

G

G

D

D

K

V

V

I

L

L

A

I

L

G

S

H

C

T

G

G

K

H

F

W

G

G

E

D

R

R

F

S

A

A

E

D

L

M

A

A

R

T

I

R

Y

Y

G

G

G

A

N

D

V

V

D

R

F

V

V

V

D

K

F

S

G

K

W

V

G

G

Y

Q

D

S

I

L

D

S

L

L

N

D

V

E

V

I

K

R

R

D

K

A

L

L

D

L

E

I

D

H

S

K

A

P

I

-

K

S

V

V

V

L

L

F

A

L

T

T

Q

Q

N

G

E

D

T
x
S

S

S

T

T

A

G

V

V

Q

L

N

Q

D

G

I

L

E

E

G

G

L

V

G

G

K

A

L

L

V

C

A

H

Q

Q

Y

H

D

N

A

C

V

L

L

L

A

I

V

V

D

D

A

T

V

V

S

A

G

S

L

L

A

G

I

A

D

P

L

M

K

F

T

M

D

D

E

R

W

W

N

E

V

I

D

D

M

A

V

M

V

Y

S

T

G

G

S

S

Q
|
Q

K
|
K

A

V

F

L

M

G

L

A

P

P

G

G

L

I

A

T

F

P

I

V

S

S

V

F

S

S

D

H

K

R

A

A

W

V

A

E

K

R

I

Y

E

K

K

R

N

R

T

N

S

T

-

K

-

V

P

K

V

V

Y

Y

F

W

D

D

L

M

K

S

K

L

A

V

K

G

K

D

S

Y

I

W

A

G

K

C

W

F

N

G

T

R

A

P

-

R

-

I

-
x
Y

-

H

Y

H

T
|
T

T

I

P

S

V

S

S

T

M

L

V

L

Y

Y

G

G

L

L

E

R

A

E

A

A

L

I

D

A

M

M

I

A

L

C

E

E

G

G

L

L

P

P

Q

A

V

L

F

I

A

A

R

R

H

H

A

E

L

D

L

C

A

A

K

K

A

R

T

L

R

Y

A

R

A

G

V

L

Q

Q

G

D

L

A

G

G

L

F

E

E

L

L

L

Y

A

A

P

D

D

P

E

K

C

D

A

R

S

L

K

S

A

T

V

V

T

T

A

T

V

I

Q

K

S

V

P

P

M

Q

V

G

V

V

D

D

A

W

D

L

T

K

L

A

R

A

K

Q

V

Y

L

A

L

M

K

K

E

T

Y

Y

G

L

V

V

T

E

F

I

A

S

G

G

Q

L

E

G

M

P

M

T

K

A

G

G

K

Q

I

V

F

F

R
|
R

I

I

A

G

H

L

M

M

G

G

SGH 78:80 (≠ SGT 63:65) binding in other chain
S155 (≠ T141) binding glyoxylate; binding L-alanine
Q205 (= Q191) binding in other chain
K206 (= K192) modified: N6-(pyridoxal phosphate)lysine
Y257 (vs. gap) binding pyridoxal 5'-phosphate
T260 (= T240) binding pyridoxal 5'-phosphate
R356 (= R336) binding glyoxylate

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
31% identity, 92% coverage: 5:359/385 of query aligns to 18:376/377 of 1vjoA

query
sites
1vjoA

K

S

K

R

Y

L

L

L

I

G

P

P

G

G

P

P

T

S

P

N

V

A

P

H

P

P

R

S

V

V

V

L

E

Q

A

A

M

M

S

N

R

V

P

S

I

P

I

V

G

G

H

H

R

L

S

D

A

P

G

A

F

F

Q

L

A

A

V

L

M

M

E

D

R

E

V

I

T

Q

G

S

K

L

L

L

K

R

K

Y

V

V

F

W

Q

Q

T

T

E

E

N

N

H

P

V

L

F

T

I

I

-

A

L

V

G

S

S

G

S
x
T

G
|
G

T
|
T

G

A

A

A

L

M

E

E

A

A

A

T

V

I

A

A

N

N

L

A

V

V

H

E

P

P

G

G

D

D

K

V

V

V

L

L

A

I

L

G

S

V

C

A

G

G

K

Y

F
|
F

G

G

E

N

R

R

F

L

A

V

E

D

L

M

A

A

R

G

I

R

Y

Y

G

G

G

A

N

D

V

V

D

R

F

T

V

I

D

S

F

K

G

P

W

W

G

G

Y

E

D

V

I

F

D

S

L

L

N

E

V

E

V

L

K

R

R

T

K

A

L

L

D

E

E

T

D

H

S

R

A

P

I

A

K

I

V

L

V

A

L

L

A

-

T

V

Q

H

N

A

E

E

T
|
T

S

S

T

T

A

G

V

A

Q

R

N

Q

D

P

I

L

E

E

G

G

L

V

G

G

K

E

L

L

V

C

A

R

Q

E

Y

F

D

G

A

T

V

L

L

L

A

L

V

V

D
|
D

A

T

V
|
V

S

T

G

S

L

L

A

G

I

V

D

P

L

I

K

F

T

L

D

D

E

A

W

W

N

G

V

V

D

D

M

L

V

A

V

Y

S

S

G

C

S

S

Q

Q

K
|
K

A

G

F

L

M

G

L

C

P

S

P

P

G

G

L

A

A

S

F

P

I

F

S

T

V

M

S

S

D

S

K

R

A

A

W

I

A

E

K

K

I

L

E

Q

K

R

N

R

T

R

S

T

P

K

V

V

-

A

-

N

Y

W

Y

Y

F

L

D

D

L

M

K

N

K

L

A

L

K

G

K

K

S

-

I

-

A

-

K

Y

W

W

N

G

T

S

A

E

-

R

-

V

-

Y

-

H

Y

H

T

T

T

A

P

P

V

I

S

N

M

L

V

Y

Y

Y

G

A

L

L

E

R

A

E

A

A

L

L

D

R

M

L

I

I

L

A

E

Q

E

E

G

G

L

L

P

A

Q

N

V

C

F

W

A

Q

R

R

H

H

A

Q

L

K

L

N

A

V

K

E

A

Y

T

L

R

W

A

E

A

R

V

L

Q

E

G

D

L

I

G

G

L

L

E

S

L

L

L

H

A

V

P

E

D

K

E

E

C

Y

A

R

S

L

K

P

A

T

V

L

T

T

A

T

V

V

Q

C

S

I

P

P

M

D

V

G

V

V

D

D

A

G

D

K

T

A

L

V

R

A

K

R

V

R

L

L

K

N

E

E

Y

H

G

N

V

I

T

E

F

V

A

G

G

G

Q

L

E

G

M

E

M

L

K

A

G

G

K

K

I

V

F

W

R

R

I

V

A

G

H

L

M

M

G

G

Y

F

A

N

G

S

K

R

M

K

D

E

V

S

I

V

I

D

A

Q

-

L

I

I

A

P

A

A

L

L

E

E

M

Q

A

V

L

L

binding pyridoxal-5'-phosphate: T77 (≠ S63), G78 (= G64), T79 (= T65), F104 (= F90), T154 (= T141), D179 (= D166), V181 (= V168), K205 (= K192)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
33% identity, 90% coverage: 1:348/385 of query aligns to 12:361/381 of 2dr1A

query
sites
2dr1A

M

M

M

V

K

K

D

P

K

K

K

Y

Y

K

L

L

L

F

I

T

P

A

G

G

P

P

T

V

P

A

V

C

P

F

P

P

R

E

V

V

V

L

E

E

A

I

M

M

S

K

R

V

P

Q

I

M

I

F

G

S

H

H

R

R

S

S

A

K

G

E

F

Y

Q

R

A

K

V

V

M

H

E

M

R

D

V

T

T

V

G

E

K

R

L

L

K

R

K

E

V

F

F

L

Q

E

T

V

E

E

N

K

-

G

H

E

V

V

F

L

I

L

L

V

G

P

S

S

S
|
S

G
|
G

T
|
T

G

G

A

I

L

M

E

E

A

A

A

S

V

I

A

R

N

N

L

G

V

V

H

S

P

K

G

G

D

G

K

K

V

V

L

L

A

V

L

T

S

I

C

I

G

G

K

A

F
|
F

G

G

E

K

R

R

F

Y

A

K

E

E

L

V

A

V

R

E

I

S

Y

N

G

G

G

R

N

K

V

A

D

V

F

V

V

L

D

E

F

Y

G

E

W

P

G

G

Y

K

D

A

I

V

D

K

L

P

N

E

V

D

V

L

K

D

R

D

K

A

L

L

D

R

E

K

D

N

S

P

A

D

I

V

K

E

V

A

V

V

L

T

A

I

T

T

Q

Y

N

N

E

E

T
|
T

S

S

T

T

A

G

V

V

Q

L

N

N

D

P

I

L

E

P

G

E

L

L

G

A

K

K

L

V

V

A

A

K

Q

E

Y

H

D

D

A

K

V

L

L

V

A

F

V

V

D
|
D

A

A

V
|
V

S

S

G

A

L

M

A

G

I

A

D

D

L

I

K

K

T

F

D

D

E

K

W

W

N

G

V

L

D

D

M

V

V

V

V

F

S

S

G
x
S

S

S

Q

Q

K
|
K

A

A

F

F

M

G

L

V

P

P

G

G

L

L

A

A

F

I

I

G

S

A

V

F

S

S

D

E

K

R

A

F

W

L

A

E

K

I

I

A

E

E

K

K

N

M

T

P

S

E

P

R

V

G

Y

W

Y

Y

F

F

D

D

L

I

K

P

K

L

A

Y

K

V

K

K

S

Y

I

L

-

K

A

E

K

K

W

E

N

S

T

T

A

P

Y

S

T

T

T

P

P

P

V

M

S

P

M

Q

V

V

Y

F

G

G

L

I

E

N

A

V

A

A

L

L

D

R

M

I

I

I

L

E

E

K

E

M

G

G

L

G

P

K

Q

E

V

K

F

W

A

L

R

E

-

M

H

Y

A

E

L

K

L

R

A

A

K

K

A

M

T

V

R

R

A

E

A

G

V

V

Q

R

G

E

L

I

G

G

L

L

E

D

L

I

L

L

A

A

P

E

D

P

E

G

C

H

A

E

S

S

K

P

A

T

V

I

T

T

A

A

V

V

Q

L

S

T

P

P

M

P

V

G

V

I

D

K

A

G

D

D

T

E

L

V

R

Y

K

E

V

A

L

M

L

R

K

K

E

R

Y

-

G

G

V

F

T

E

F

L

A

A

G

K

G

G

Q

Y

E

G

M

S

M

V

K

K

G

E

K

K

I

T

F

F

R

R

I

I

A

G

H

H

M

M

G

G

Y

Y

A

M

G

K

K

F

M

E

D

D

V

I

binding pyridoxal-5'-phosphate: S75 (= S63), G76 (= G64), T77 (= T65), F102 (= F90), T153 (= T141), D178 (= D166), V180 (= V168), S201 (≠ G189), K204 (= K192)

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
31% identity, 93% coverage: 6:362/385 of query aligns to 19:380/383 of 3kgxA

query
sites
3kgxA

K

R

Y

L

L

L

I

G

P

P

G

G

P

P

T

S

P

N

V

L

P

A

P

P

R

R

V

V

V

L

E

A

A

A

M

G

S

S

R

L

P

R

I

M

I

I

G

G

H

H

R

M

S

Q

A

K

G

E

F

M

Q

L

A

Q

V

I

M

M

E

E

R

E

V

I

T

K

G

Q

K

G

L

I

K

Q

K

Y

V

V

F

F

Q

Q

T

T

E

R

N

N

H

P

V

L

-

T

F

L

I

V

L

V

G

S

S

G

S

S

G

G

T

H

G

C

A

A

L

M

E

E

A

T

A

A

V

L

A

F

N

N

L

L

V

L

H

E

P

P

G

G

D

D

K

S

V

F

L

L

A

T

L

G

S

T

C

N

G

G

K

I

F

W

G

G

E

M

R

R

F

A

A

A

E

E

L

I

A

A

R

D

I

R

Y

I

G

G

G

A

N

R

V

V

D

H

F

Q

V

M

D

I

F

K

G

K

W

P

G

G

Y

E

D

H

I

Y

D

T

L

L

N

Q

V

E

V

V

K

E

R

E

K

G

L

L

D

A

E

Q

D

H

S

K

A

P

I

V

K

L

V

L

V

F

L

L

A

V

T

-

Q

H

N

G

E

E

T

S

S

S

T

T

A

G

V

V

Q

V

N

Q

D

P

I

L

E

D

G

G

L

F

G

G

K

E

L

L

V

C

A

H

Q

R

Y

Y

D

Q

A

C

V

L

L

L

A

L

V

V

D

D

A

S

V

V

S

A

G

S

L

L

A

G

I

V

D

P

L

I

K

Y

T

M

D

D

E

Q

W

Q

N

G

V

I

D

D

M

I

V

M

V

Y

S

S

G

S

S

S

Q

Q

K

K

A

V

F

L

M

N

L

A

P

P

G

G

L

I

A

S

F

L

I

I

S

S

V

F

S

N

D

D

K

K

A

A

W

K

A

Y

K

K

I

V

-

Y

E

S

K

R

N

K

T

T

S

K

P

P

V

V

-

S

Y

F

Y

Y

F

T

D

D

L

I

K

T

K

Y

A

L

K

A

K

K

S

-

I

-

A

-

K

L

W

W

N

G

T

C

A

E

-

G

-

V

-

I

-

H

Y

H

T

T

P

P

V

V

S

T

M

S

V

L

Y

Y

G

C

L

L

E

R

A

E

A

S

L

L

D

A

M

L

I

I

L

A

E

E

E

Q

G

G

L

L

P

E

Q

N

V

C

F

W

A

R

R

R

H

H

A

R

L

E

L

A

A

T

K

A

A
x
H

T

L

R

H

A

K

A
x
H

V

L

Q

Q

G

E

L

M

G

G

L

L

E

K

L

F

A

V

P

K

D

D

-

P

E

E

C

I

A

R

S

L

K

P

A

T

V

I

T

T

A

T

V

V

Q

T

S

V

P

P

M

A

V

G

V

Y

D

N

A

W

D

R

T

D

L

I

R

V

K

S

V

Y

L

V

L

L

K

D

E

H

Y

F

G

S

V

I

T

E

F

I

A

S

G

G

Q

L

E

G

M

P

M

T

K

E

G

E

K

R

I

V

F

L

R

R

I

I

A

G

H

L

M

L

G

G

Y

Y

A

N

G

A

K

T

M

T

D

E

V

N

I

V

I
x
D

A

R

I

V

A

A

-

E

A

A

L

L

E

R

M

E

A

A

L

L

G

Q

K

H

C

C

binding magnesium ion: H290 (≠ A274), H294 (≠ A278), D367 (≠ I350)

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
31% identity, 93% coverage: 6:362/385 of query aligns to 23:385/388 of 3kgwB

query
sites
3kgwB

K

R

Y

L

L

L

I

G

P

P

G

G

P

P

T

S

P

N

V

L

P

A

P

P

R

R

V

V

V

L

E

A

A

A

M

G

S

S

R

L

P

R

I

M

I

I

G

G

H

H

R

M

S

Q

A

K

G

E

F

M

Q

L

A

Q

V

I

M

M

E

E

R

E

V

I

T

K

G

Q

K

G

L

I

K

Q

K

Y

V

V

F

F

Q

Q

T

T

E

R

N

N

H

P

V

L

-

T

F

L

I

V

L

V

G

S

S

G

S
|
S

G
|
G

T
x
H

G

C

A

A

L

M

E

E

A

T

A

A

V

L

A

F

N

N

L

L

V

L

H

E

P

P

G

G

D

D

K

S

V

F

L

L

A

T

L

G

S

T

C

N

G

G

K

I

F
x
W

G

G

E

M

R

R

F

A

A

A

E

E

L

I

A

A

R

D

I

R

Y

I

G

G

G

A

N

R

V

V

D

H

F

Q

V

M

D

I

F

K

G

K

W

P

G

G

Y

E

D

H

I

Y

D

T

L

L

N

Q

V

E

V

V

K

E

R

E

K

G

L

L

D

A

E

Q

D

H

S

K

A

P

I

V

K

L

V

L

V

F

L

L

A

V

T

-

Q

H

N

G

E

E

T
x
S

S

S

T

T

A

G

V

V

Q

V

N

Q

D

P

I

L

E

D

G

G

L

F

G

G

K

E

L

L

V

C

A

H

Q

R

Y

Y

D

Q

A

C

V

L

L

L

A

L

V

V

D
|
D

A

S

V
|
V

S

A

G

S

L

L

A

G

I

V

D

P

L

I

K

Y

T

M

D

D

E

Q

W

Q

N

G

V

I

D

D

M

I

V

M

V

Y

S

S

G

S

S

S

Q

Q

K
|
K

A

V

F

L

M

N

L

A

P

P

G

G

L

I

A

S

F

L

I

I

S

S

V

F

S

N

D

D

K

K

A

A

W

K

A

Y

K

K

I

V

-

Y

E

S

K

R

N

K

T

T

S

K

P

P

V

V

-

S

Y

F

Y

Y

F

T

D

D

L

I

K

T

K

Y

A

L

K

A

K

K

S

-

I

-

A

-

K

L

W

W

N

G

T

C

A

E

-

T

-

R

-

V

-

I

-

H

Y

H

T

T

P

P

V

V

S

T

M

S

V

L

Y

Y

G

C

L

L

E

R

A

E

A

S

L

L

D

A

M

L

I

I

L

A

E

E

E

Q

G

G

L

L

P

E

Q

N

V

C

F

W

A

R

R

R

H

H

A

R

L

E

L

A

A

T

K

A

A

H

T

L

R

H

A

K

A

H

V

L

Q

Q

G

E

L

M

G

G

L

L

E

K

L

F

A

V

P

K

D

D

-

P

E

E

C

I

A

R

S

L

K

P

A

T

V

I

T

T

A

T

V

V

Q

T

S

V

P

P

M

A

V

G

V

Y

D

N

A

W

D

R

T

D

L

I

R

V

K

S

V

Y

L

V

L

L

K

D

E

H

Y

F

G

S

V

I

T

E

F

I

A

S

G

G

Q

L

E

G

M

P

M

T

K

E

G

E

K

R

I

V

F

L

R

R

I

I

A

G

H

L

M

L

G

G

Y

Y

A

N

G

A

K

T

M

T

D

E

V

N

I

V

I

D

A

R

I

V

A

A

-

E

A

A

L

L

E

R

M

E

A

A

L

L

G

Q

K

H

C

C

binding pyridoxal-5'-phosphate: S81 (= S63), G82 (= G64), H83 (≠ T65), W108 (≠ F90), S158 (≠ T141), D183 (= D166), V185 (= V168), K209 (= K192)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
31% identity, 94% coverage: 5:365/385 of query aligns to 22:390/392 of P21549

query
sites
P21549

K

N

K

Q

Y

L

L

L

I

G

P

P

G

G

P

P

T

S

P

N

V

L

P

P

R
|
R

V

I

V

M

E

A

A

A

M
x
G

S

G

R

L

P

Q

I

M

I

I

G
|
G

H

S

R

M

S

S

A

K

G

D

F

M

Q

Y

A

Q

V

I

M

M

E

D

R

E

V

I

T

K

G

E

K

G

L

I

K

Q

K

Y

V

V

F

F

Q

Q

T

T

E

R

N

N

H

P

V

L

-

T

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R36 (= R19) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ M24) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (= G30) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G64) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F90) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ F94) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ K132) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ V134) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ N139) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T141) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (≠ A144) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ I149) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (= G153) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ V156) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D166) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (≠ G170) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ M185) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S188) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K192) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ A201) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ K215) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ L225) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ T237) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (= I256) to T: in dbSNP:rs140992177
A280 (≠ L257) to V: in dbSNP:rs73106685
V326 (≠ S302) to I: in dbSNP:rs115057148
I340 (≠ L316) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
31% identity, 94% coverage: 5:365/385 of query aligns to 17:385/385 of 5hhyA

query
sites
5hhyA

K

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binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (= S63), G77 (= G64), H78 (≠ T65), W103 (≠ F90), S153 (≠ T141), D178 (= D166), V180 (= V168), Q203 (= Q191), K204 (= K192), Y255 (vs. gap), T258 (= T240)

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
31% identity, 93% coverage: 5:362/385 of query aligns to 17:382/384 of 6rv0A

query
sites
6rv0A

K

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L

L

L

F

A

V

P

K

D

D

E

P

C

A

A

L

S

R

-

L

K

P

A

T

V

V

T

T

A

T

V

V

Q

A

S

V

P

P

M

A

V

G

V

Y

D

D

A

W

D

R

T

D

L

I

R

V

K

S

V

Y

L

V

L

I

K

D

E

H

Y

F

G

D

V

I

T

E

F

I

A

M

G

G

Q

L

E

G

M

P

M

S

K

T

G

G

K

K

I

V

F

L

R

R

I

I

A

G

H

L

M

L

G

G

Y

C

-

N

A

A

G

T

K

R

M

E

D

N

V

V

I

D

I

R

A

V

I

T

A

E

A

A

L

L

E

R

M

A

A

A

L

L

G

Q

K

H

C

C

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (= S63), G77 (= G64), H78 (≠ T65), W103 (≠ F90), D178 (= D166), V180 (= V168), A181 (≠ S169), K204 (= K192)

Query Sequence

>WP_013840109.1 NCBI__GCF_000215085.1:WP_013840109.1
MMKDKKYLLIPGPTPVPPRVVEAMSRPIIGHRSAGFQAVMERVTGKLKKVFQTENHVFIL
GSSGTGALEAAVANLVHPGDKVLALSCGKFGERFAELARIYGGNVDFVDFGWGYDIDLNV
VKRKLDEDSAIKVVLATQNETSTAVQNDIEGLGKLVAQYDAVLAVDAVSGLAAIDLKTDE
WNVDMVVSGSQKAFMLPPGLAFISVSDKAWAKIEKNTSPVYYFDLKKAKKSIAKWNTAYT
TPVSMVYGLEAALDMILEEGLPQVFARHALLAKATRAAVQGLGLELLAPDECASKAVTAV
QSPMVVDADTLRKVLLKEYGVTFAGGQEMMKGKIFRIAHMGYAGKMDVIIAIAALEMALG
KCGYKAELGAGVREAQMVFVGGEHA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory