SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013840348.1 NCBI__GCF_000215085.1:WP_013840348.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
52% identity, 72% coverage: 5:168/228 of query aligns to 81:244/250 of 4hzdA

query
sites
4hzdA

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I

binding coenzyme a: H158 (= H82), G203 (≠ S127), A204 (≠ S128), A221 (≠ G145), A222 (= A146), T235 (= T159), A237 (≠ V161), I244 (≠ V168)
binding magnesium ion: S224 (= S148), A237 (≠ V161), G238 (= G162)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
44% identity, 85% coverage: 5:198/228 of query aligns to 82:261/261 of 6wyeA

query
sites
6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D93 (= D16), P94 (= P17), D158 (= D81), H159 (= H82), G185 (= G108), R193 (= R116), H194 (= H117)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
48% identity, 72% coverage: 5:168/228 of query aligns to 80:243/243 of 7ra4A

query
sites
7ra4A

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binding serine: D91 (= D16), P92 (= P17), D156 (= D81), H157 (= H82), R191 (= R116), H192 (= H117)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
48% identity, 79% coverage: 1:181/228 of query aligns to 73:252/258 of 8i04A

query
sites
8i04A

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binding serine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R116), H189 (= H117)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
49% identity, 74% coverage: 1:168/228 of query aligns to 76:243/246 of 8i09A

query
sites
8i09A

M

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binding cysteine: D91 (= D16), D156 (= D81), H157 (= H82), G164 (= G89), R191 (= R116), H192 (= H117)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G108), T184 (= T109), G202 (≠ S127), K218 (= K143), I219 (= I144), G220 (= G145), L226 (= L151), A236 (≠ V161)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
49% identity, 74% coverage: 1:168/228 of query aligns to 77:244/244 of 8i06A

query
sites
8i06A

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binding coenzyme a: H158 (= H82), L177 (≠ Y101), Q178 (= Q102), G203 (≠ S127), A204 (≠ S128), G221 (= G145), A222 (= A146), T235 (= T159), A237 (≠ V161)
binding cysteine: P93 (= P17), H158 (= H82)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
48% identity, 74% coverage: 1:168/228 of query aligns to 77:244/262 of 1t3dA

query
sites
1t3dA

M

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G

R

N

E

N

G

V

V

V

M

I

I

S

G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

S

N

F

I

Q

E

V

V

G

G

E

R

N

G

V

A

K

K

I

I

G

G

A

A

G

G

S

S

V

V

V

V

L

L

K

Q

P

P

V

V

P

P

P

P

N

H

C

T

T

T

V

A

V

A

G

G

V

V

P

P

G

A

R

R

I

I

V

V

binding cysteine: D92 (= D16), P93 (= P17), D157 (= D81), G184 (= G108), R192 (= R116), H193 (= H117)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
49% identity, 76% coverage: 8:181/228 of query aligns to 80:252/258 of 4h7oA

query
sites
4h7oA

E

D

I

I

H

C

A

A

V

T

F

V

E

N

R

R

D
|
D

P
|
P

A

A

A

V

K

S

S

M

V

Y

I

S

E

M

V

P

L

L

L

L

C

Y

Y

L

P

K

G

G

L

Y

H

H

A

A

I

L

L

Q

F

G

H

Y

R

R

V

V

A

A

H

N

A

W

L

L

Y

W

K

R

K

Q

-

G

K

R

F

K

F

A

V

L

T

A

A

T

R
x
Y

F

F

L

Q

S

N

Q
|
Q

I

I

S

S

R

V

W

-

L

A

T

C

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

I

I

G

G

E

R

G

G

L

I

F

M

I

L

D
|
D

H
|
H

G

A

S

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

D

N

D

V

V

T

S

I

I

Y

L

Q

Q

G

D

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

E

K

C

G

G

K

D

R
|
R

H
|
H

P

P

T

K

I

I

G

R

N

E

N

G

V

V

V

M

I

I

S

G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

S

N

F

I

Q

E

V

V

G

G

E

E

N

G

V

A

K

K

I

I

G

G

A

S

G

G

S

S

V

V

V

V

L

L

K

Q

P

A

V

V

P

P

P

P

N

H

C

T

T

T

V

V

V

A

G

G

V

V

P

P

G

A

R

R

I

I

V

V

I

G

R

R

D

P

G

-

Q

Q

K

S

V

D

S

K

P

P

V

S

Q

L

D

D

L

M

binding arginine: Y127 (≠ R54), Q131 (= Q58)
binding cysteine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R116), H189 (= H117)

Sites not aligning to the query:

binding arginine: 51, 53

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
46% identity, 77% coverage: 8:182/228 of query aligns to 80:253/257 of 1ssqD

query
sites
1ssqD

E

D

I

I

H

Q

A

A

V

V

F

R

E

H

R

R

D
|
D

P

P

A

A

A

V

K

E

S

L

V

W

I

S

E

T

V

P

L

L

L

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

I

L

Q

F

S

H

Y

R

R

V

I

A

T

H

H

A

Y

L

L

Y

W

K

N

K

Q

K

N

F

R

F

K

V

S

T

L

A

A

R

L

F

Y

L

L

S

Q

Q

N

I

Q

S

I

R

S

W

V

L

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

K

I

I

G

G

E

H

G

G

L

I

F

M

I

F

D
|
D

H
|
H

G

A

S

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

N

D

V

V

T

S

I

I

Y

L

Q

Q

G

G

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

E

K

S

G

G

K

D

R

R

H

H

P

P

T

K

I

V

G

R

N

E

N

G

V

V

V

M

I

I

S

G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

S

N

F

I

Q

E

V

V

G

G

E

K

N

Y

V

A

K

K

I

I

G

G

A

A

G

N

S

S

V

V

V

V

L

L

K

N

P

P

V

V

P

P

P

E

N

Y

C

A

T

T

V

A

V

A

G

G

V

V

P

P

G

A

R

R

I

I

V

V

I

S

R

Q

D

D

G

-

Q

K

K

A

V

A

S

K

P

P

V

A

Q

F

D

D

L

M

R

N

binding cysteine: D88 (= D16), D153 (= D81), H154 (= H82)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
50% identity, 71% coverage: 8:168/228 of query aligns to 83:243/243 of 4n69A

query
sites
4n69A

E

D

I

L

H

R

A

A

V

A

F

R

E

E

R

R

D

D

P

P

A

A

A

C

K

V

S

S

V

Y

I

S

E

H

V

C

L

L

L

L

C

N

Y

Y

P

K

G

G

L

F

H

L

A

A

I

C

L

Q

F

A

H

H

R

R

V

V

A

A

H

H

A

L

L

L

Y

W

K

R

K

Q

K

S

F

R

F

R

V

P

T

L

A

A

R

L

F

A

L

L

S

H

Q

S

I

R

S

I

R

A

W

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

I

I

G

G

E

K

G

G

L

I

F

L

I

F

D

D

H

H

G

A

S

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

N

N

V

V

T

S

I

I

Y

L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

I

I

G

G

N

D

N

G

V

V

V

L

I

I

S

G

S

A

G

G

A

A

K

T

V

I

L

L

G

G

S

N

F

I

Q

K

V

I

G

G

E

E

N

G

V

A

K

K

I

V

G

G

A

A

G

G

S

S

V

V

V

V

L

L

K

I

P

D

V

V

P

P

P

P

N

R

C

T

T

T

V

A

V

V

G

G

V

N

P

P

G

A

R

R

I

L

V

V

binding serine: G183 (= G108), R191 (= R116), H192 (= H117)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
45% identity, 81% coverage: 1:185/228 of query aligns to 80:261/272 of 3gvdI

query
sites
3gvdI

M

M

F

I

G

V

R

S

I

A

K

A

R

R

E

D

I

I

H

L

A

A

V

V

F

R

E

L

R

R

D
|
D

P
|
P

A

A

A

V

K

D

S

K

V

Y

I

S

E

T

V

P

L

L

L

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

L

L

Q

F

A

H

Y

R

R

V

I

A

G

H

H

A

W

L

L

Y

W

K

A

K

Q

K

D

F

R

F

K

V

A

T

L

A

A

R

I

F

Y

L

L

S

Q

Q

N

I

Q

S

V

R

S

W

V

L

A

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

T

I

I

G

G

E

C

G

G

L

I

F

M

I

L

D
|
D

H

H

G

A

S

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

N

N

D

V

V

T

S

I

I

Y

L

Q

Q

G

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

G

G

K

K

E

T

K

S

G

G

K

D

R
|
R

H

H

P

P

T

K

I

I

G

R

N

E

N

G

V

V

V

M

I

I

S

G

S

A

G

G

A

A

K

K

V

I

L

L

G

G

S

N

F

I

Q

E

V

V

G

G

E

R

N

G

V

A

K

K

I

I

G

G

A

A

G

G

S

S

V

V

V

V

L

L

K

Q

P

S

V

V

P

P

P

A

N

H

C

T

T

T

V

A

V

A

G

G

V

V

P

P

G

A

R

R

I

I

V

V

I

-

R

-

D

-

G

G

Q

K

K

P

V

E

S

S

P

D

V

K

Q

P

D

S

L

L

R

D

H

M

D

D

Q

Q

binding cysteine: D95 (= D16), P96 (= P17), D160 (= D81), G186 (= G107), G187 (= G108), R195 (= R116)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
49% identity, 66% coverage: 5:154/228 of query aligns to 103:252/280 of 7bw9A

query
sites
7bw9A

I

V

K

K

R

Q

E

D

I

I

H

I

A

A

V

A

F

Y

E

T

R

G

D
|
D

P
|
P

A

A

A

A

K

S

S

S

V

L

I

A

E

M

V

I

L

I

L

R

C

S

Y

Y

P

P

G

G

L

I

H

H

A

V

I

M

L

M

F

I

H

Q

R

R

V

V

A

A

H

H

A

I

L

L

Y

Y

K

M

K

N

K

G

F

D

F

I

V

E

T

Y

A

S

R

R

F

E

L

L

S

M

Q

E

I

N

S

I

R

H

W

S

L

V

T

T

G

G

I

I

E

D

I

I

H

H

P

P

G

G

A

T

K

S

I

I

G

G

E

N

G

H

L

F

F

F

I

I

D
|
D

H
|
H

G

G

S

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

N

W

V

C

T

R

I

V

Y

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

G

S

K

F

E

K

K

G

G

N

K

K

R
|
R

H
|
H

P

P

T

T

I

I

G

G

N

D

N

F

V

V

V

V

I

I

S

G

S

A

G

G

A

A

K

K

V

V

L

L

G

G

S

N

F

I

Q

T

V

I

G

G

E

S

N

N

V

V

K

K

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

T

K

Q

P

N

V

I

binding cysteine: D114 (= D16), P115 (= P17), D179 (= D81), H180 (= H82), A206 (≠ G108), R214 (= R116), H215 (= H117)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
48% identity, 71% coverage: 8:168/228 of query aligns to 79:233/233 of 4n6bA

query
sites
4n6bA

E

D

I

L

H

R

A

A

V

A

F

R

E

E

R

R

D

D

P

-

A

-

A

-

K

V

S

S

V

Y

I

S

E

H

V

C

L

L

L

L

C

N

Y

Y

P

K

G

G

L

F

H

L

A

A

I

C

L

Q

F

A

H

H

R

R

V

V

A

A

H

H

A

L

L

L

Y

W

K

R

K

Q

K

S

F

R

F

R

V

P

T

L

A

A

R

L

F

A

L

L

S

H

Q

S

I

R

S

I

R

A

W

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

I

I

G

G

E

K

G

G

L

I

F

L

I

F

D

D

H
|
H

G

A

S

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

N

N

V

V

T

S

I

I

Y
x
L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

-

E

-

K

-

G

G

K

D

R

R

H

H

P

P

T

K

I

I

G

G

N

D

N

G

V

V

V

L

I

I

S

G

S

A

G

G

A

A

K

T

V

I

L

L

G

G

S

N

F

I

Q

K

V

I

G

G

E

E

N

G

V

A

K
|
K

I

V

G
|
G

A
|
A

G

G

S

S

V

V

V

V

L
|
L

K

I

P

D

V

V

P

P

P

P

N

R

C

T

T

T

V

A

V
|
V

G

G

V

N

P

P

G

A

R

R

I

L

V

V

binding coenzyme a: H150 (= H82), L169 (≠ Y101), G176 (= G108), K208 (= K143), G210 (= G145), A211 (= A146), L216 (= L151), V226 (= V161)

1sstA Serine acetyltransferase- complex with coa (see paper)
45% identity, 71% coverage: 8:168/228 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

E

D

I

I

H

Q

A

A

V

V

F

R

E

H

R

R

D

D

P

P

A

A

A

V

K

E

S

L

V

W

I

S

E

T

V

P

L

L

L

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

I

I

L

Q

F

S

H

Y

R

R

V

I

A

T

H

H

A

Y

L

L

Y

W

K

N

K

Q

K

N

F

R

F

K

V

S

T

L

A

A

R

L

F

Y

L

L

S

Q

Q

N

I

Q

S

I

R

S

W

V

L

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

K

I

I

G

G

E

H

G

G

L

I

F

M

I

F

D

D

H
|
H

G

A

S

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

N

D

V

V

T

S

I

I

Y
x
L

Q

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

K

-

G

-

K

-

R

-

H

H

P

P

T

K

I

V

G

R

N

E

N

G

V

V

V

M

I

I

S

G

S
x
A

G

G

A

A

K

K

V

I

L
|
L

G

G

S

N

F

I

Q

E

V

V

G

G

E

K

N

Y

V

A

K
|
K

I

I

G
|
G

A
|
A

G

N

S

S

V
|
V

V

V

L
|
L

K

N

P

P

V

V

P

P

P

E

N

Y

C

A

T
|
T

V

A

V
x
A

G

G

V
|
V

P
|
P

G

A

R

R

I

I

V
|
V

binding coenzyme a: H154 (= H82), L173 (≠ Y101), G180 (= G108), T181 (= T109), A193 (≠ S128), L198 (= L133), K208 (= K143), G210 (= G145), A211 (= A146), V214 (= V149), L216 (= L151), T224 (= T159), A226 (≠ V161), V228 (= V163), P229 (= P164), V233 (= V168)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
45% identity, 68% coverage: 5:160/228 of query aligns to 105:268/270 of 3p47A

query
sites
3p47A

I

L

K

K

R

T

E

D

I

L

H

I

A

A

V

A

F

Y

E

A

R

G

D

D

P

P

A

A

A

A

K

P

S

G

V

L

I

S

E

L

V

I

L

I

L

R

C

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

I

V

L

I

F

V

H

Y

R

R

V

I

A

A

H

H

A

V

L

L

Y

Y

K

E

K

C

K

G

F

E

F

R

V

Y

T

Y

A

C

R

R

F

E

L

M

S

M

Q

E

I

S

S

V

R

H

W

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

K

S

I

I

G

K

E

G

G

H

L

F

F

F

I

I

D

D

H

H

G

G

S

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

N

W

V

C

T

R

I

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

K

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

I

V

G

G

N

D

N

Y

V

V

V

T

I

I

S

G

S

T

G

G

A

A

K

K

V

V

L

L

G

G

S

N

F

I

Q

I

V

V

G

G

E

S

N

H

V

V

K

R

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

P

S

N

N

C

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R116), H225 (= H117)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
45% identity, 68% coverage: 5:160/228 of query aligns to 103:266/267 of 3q1xA

query
sites
3q1xA

I

L

K

K

R

T

E

D

I

L

H

I

A

A

V

A

F

Y

E

A

R

G

D

D

P

P

A

A

A

A

K

P

S

G

V

L

I

S

E

L

V

I

L

I

L

R

C

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

I

V

L

I

F

V

H

Y

R

R

V

I

A

A

H

H

A

V

L

L

Y

Y

K

E

K

C

K

G

F

E

F

R

V

Y

T

Y

A

C

R

R

F

E

L

M

S

M

Q

E

I

S

S

V

R

H

W

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

K

S

I

I

G

K

E

G

G

H

L

F

F

F

I

I

D

D

H

H

G

G

S

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

N

W

V

C

T

R

I

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

K

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

I

V

G

G

N

D

N

Y

V

V

V

T

I

I

S

G

S

T

G

G

A

A

K

K

V

V

L

L

G

G

S

N

F

I

Q

I

V

V

G

G

E

S

N

H

V

V

K

R

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

P

S

N

N

C

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R116), H223 (= H117)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
36% identity, 48% coverage: 74:182/228 of query aligns to 77:197/201 of 1krvA

query
sites
1krvA

I

I

G

G

E

R

G

N

L

F

F
x
Y

I

A

D
x
N

H

F

G

N

S

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

N

N

V

V

T

L

I

I

Y
x
A

Q

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

K

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

I

I

G

G

N

N

N

N

V

V

V
x
W

I

I

S
x
G

S
|
S

G

H

A

V

K

V

V

I

L
x
N

G

P

S

G

F

V

Q

T

V

I

G

G

E

D

N

N

V

S

K

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

P

P

N

N

C

V

T

V

V

A

V

A

G

G

V

V

P
|
P

G

C

R
|
R

I

V

V

I

-
x
R

-

E

I

I

R

N

D

D

G

R

Q

D

K

K

V

H

S

Y

P

Y

V

F

Q

K

D

D

L

Y

R

K

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), M126 (≠ R116)
binding coenzyme a: A104 (≠ Y101), S110 (≠ G107), T112 (= T109), W138 (≠ V125), G140 (≠ S127), S141 (= S128), N146 (≠ L133), A159 (= A146), P177 (= P164), R179 (= R166), R182 (vs. gap)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
36% identity, 48% coverage: 74:182/228 of query aligns to 77:197/201 of 1kruA

query
sites
1kruA

I

I

G

G

E

R

G

N

L

F

F
x
Y

I

A

D
x
N

H

F

G

N

S

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

N

N

V

V

T
x
L

I

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

K

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

I

I

G

G

N

N

N

N

V

V

V
x
W

I

I

S
x
G

S
|
S

G

H

A

V

K

V

V

I

L

N

G

P

S

G

F

V

Q

T

V

I

G

G

E

D

N

N

V

S

K

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

P

P

N

N

C

V

T

V

V

A

V

A

G

G

V

V

P
|
P

G

C

R

R

I

V

V

I

-
x
R

-

E

I

I

R

N

D

D

G

R

Q

D

K

K

V

H

S

Y

P

Y

V

F

Q

K

D

D

L

Y

R

K

binding coenzyme a: H114 (vs. gap), W138 (≠ V125), G140 (≠ S127), S141 (= S128), G158 (= G145), A159 (= A146), P177 (= P164), R182 (vs. gap)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F79), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), L102 (≠ T99), H114 (vs. gap), M126 (≠ R116)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 48% coverage: 74:182/228 of query aligns to 78:198/203 of P07464

query
sites
P07464

I

I

G

G

E

R

G

N

L

F

F

Y

I

A

D
x
N

H

F

G

N

S

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

N

N

V

V

T

L

I

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

K

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

I

I

G

G

N

N

N

N

V

V

V

W

I

I

S

G

S
|
S

G

H

A

V

K

V

V

I

L

N

G

P

S

G

F

V

Q

T

V

I

G

G

E

D

N

N

V

S

K

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

L
x
T

K
|
K

P

D

V

I

P

P

P

P

N

N

C

V

T

V

V

A

V

A

G

G

V

V

P

P

G

C

R
|
R

I

V

V

I

-
x
R

-

E

I

I

R

N

D

D

G

R

Q

D

K

K

V

H

S

Y

P

Y

V

F

Q

K

D

D

L

Y

R

K

N85 (≠ D81) binding in other chain; binding in other chain
D93 (≠ G89) binding substrate
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S128) binding in other chain
A160 (= A146) binding in other chain
TK 165:166 (≠ LK 151:152) binding acetyl-CoA
R180 (= R166) binding acetyl-CoA
R183 (vs. gap) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
36% identity, 48% coverage: 74:182/228 of query aligns to 77:197/200 of 1krrA

query
sites
1krrA

I

I

G

G

E

R

G

N

L

F

F

Y

I

A

D
x
N

H

F

G

N

S

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

N

N

V

V

T

L

I
|
I

Y
x
A

Q
x
P

G

N

V

V

T

T

L

L

G

S

G

V

T
|
T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

K

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

I

I

G

G

N

N

N

N

V

V

V

W

I

I

S
x
G

S
|
S

G

H

A

V

K

V

V

I

L
x
N

G

P

S

G

F

V

Q

T

V

I

G

G

E

D

N

N

V

S

K

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

P

P

N

N

C

V

T

V

V

A

V
x
A

G

G

V

V

P
|
P

G

C

R

R

I

V

V

I

-
x
R

-

E

I

I

R

N

D

D

G

R

Q

D

K

K

V

H

S

Y

P

Y

V

F

Q

K

D

D

L

Y

R

K

binding acetyl coenzyme *a: N84 (≠ D81), I103 (= I100), A104 (≠ Y101), P105 (≠ Q102), T112 (= T109), H114 (vs. gap), G140 (≠ S127), S141 (= S128), N146 (≠ L133), G158 (= G145), A159 (= A146), A174 (≠ V161), P177 (= P164), R182 (vs. gap)

Query Sequence

>WP_013840348.1 NCBI__GCF_000215085.1:WP_013840348.1
MFGRIKREIHAVFERDPAAKSVIEVLLCYPGLHAILFHRVAHALYKKKFFVTARFLSQIS
RWLTGIEIHPGAKIGEGLFIDHGSGVVIGETAEIGDNVTIYQGVTLGGTGKEKGKRHPTI
GNNVVISSGAKVLGSFQVGENVKIGAGSVVLKPVPPNCTVVGVPGRIVIRDGQKVSPVQD
LRHDQLPDPIADMLAQMQNTIEKLEQRVKQLEPAEDILHRESGGGRGD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory