SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013840816.1 NCBI__GCF_000215085.1:WP_013840816.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
66% identity, 98% coverage: 1:305/311 of query aligns to 1:305/310 of P9WP55

query
sites
P9WP55

M

M

T

S

I

I

Y

A

N

E

N

D

I

I

L

T

E

Q

T

L

I

I

G

G

G

R

T

T

P

P

L

L

V

V

R

R

V

L

N

R

R

R

L

V

A

T

A

D

G

G

T

A

A

V

A

A

E

D

V

I

L

V

A

A

K

K

V

L

E

E

F

F

N

N

P

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

V

A

A

M

M

I

L

M

Q

D

A

A

A

L

E

E

Q

R

A

G

G

V

L

I

I

D

K

K

P

D

D

S

T

V

I

L

I

V

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

A

L

L

A

A

L

M

T

V

C

C

A

A

L

R

G

G

L

Y

R

R

L

C

I

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

L

E

E

R

R

S

M

L

L

Q

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

G

G

R

A

E

D

G

G

M

M

N

S

G

G

A

A

I

I

A

A

R

K

A

A

E

E

L

L

V

A

K

K

S

T

T

D

P

Q

G

R

A

Y

L

F

M

V

L

P

Q

Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

A

A

I

H

H

V

R

R

V

N

T

T

T

A

A

V

E

E

E

I

V

W

W

E

R

H

D

T

T

G

D

G

G

K

K

V

V

D

D

I

I

F

V

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

T

Q

Y

V

I

I

K

K

E

E

R

R

K

K

P

P

S

S

F

A

Q

R

A

F

I

V

A

A

V

V

E

E

P

P

A

A

E

A

S

S

P

P

V

V

L

L

S

S

G

G

Q

Q

P

K

G

G

S

P

H

H

P

P

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

K

P

V

V

L

L

N

D

L

Q

A

D

M

L

I

V

D

D

E

E

I

I

F

I

Q

T

V

V

N

G

G

N

E

E

K

D

A

A

M

L

E

N

T

V

A

A

R

R

V

L

A

A

R

R

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

T

Y

V

A

A

L

L

E

Q

V

V

A

A

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

V

L

V

L

L

P

P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
N74 (= N74) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
S266 (= S266) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
66% identity, 98% coverage: 1:305/311 of query aligns to 1:305/306 of 2q3dA

query
sites
2q3dA

M

M

T

S

I

I

Y

A

N

E

N

D

I

I

L

T

E

Q

T

L

I

I

G

G

G

R

T

T

P

P

L

L

V

V

R

R

V

L

N

R

R

R

L

V

A

T

A

D

G

G

T

A

A

V

A

A

E

D

V

I

L

V

A

A

K

K

V

L

E

E

F

F

N

N

P

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

V

A

A

M

M

I

L

M

Q

D

A

A

A

L

E

E

Q

R

A

G

G

V

L

I

I

D

K

K

P

D

D

S

T

V

I

L

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

V

I

G

A

L

L

A

A

L

M

T

V

C

C

A

A

L

R

G

G

L

Y

R

R

L

C

I

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

L

E

E

R

R

S

M

L

L

Q

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

G

G

R

A

E

D

G

G

M

M

N

S

G

G

A

A

I

I

A

A

R

K

A

A

E

E

L

L

V

A

K

K

S

T

T

D

P

Q

G

R

A

Y

L

F

M

V

L

P

Q

Q

Q
|
Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

A

A

I

H

H

V

R

R

V

N

T

T

T

A

A

V

E

E

E

I

V

W

W

E

R

H

D

T

T

G

D

G

G

K

K

V

V

D

D

I

I

F

V

V

V

A

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

T

Q

Y

V

I

I

K

K

E

E

R

R

K

K

P

P

S

S

F

A

Q

R

A

F

I

V

A

A

V

V

E

E

P

P

A

A

E

A

S

S

P

P

V

V

L

L

S

S

G

G

Q

Q

P

K

G

G

S

P

H

H

P

P

I

I

Q

Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

V

V

P

P

K

P

V

V

L

L

N

D

L

Q

A

D

M

L

I

V

D

D

E

E

I

I

F

I

Q

T

V

V

N

G

G

N

E

E

K

D

A

A

M

L

E

N

T

V

A

A

R

R

V

L

A

A

R

R

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

T

Y

V

A

A

L

L

E

Q

V

V

A

A

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

V

L

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K44), T71 (= T71), S72 (= S72), N74 (= N74), T75 (= T75), Q144 (= Q144), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G222), I223 (= I223), S266 (= S266), P293 (= P293), D294 (= D294)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
65% identity, 96% coverage: 1:300/311 of query aligns to 1:300/300 of 3zeiA

query
sites
3zeiA

M

M

T

S

I

I

Y

A

N

E

N

D

I

I

L

T

E

Q

T

L

I

I

G

G

G

R

T

T

P

P

L

L

V

V

R

R

V

L

N

R

R

R

L

V

A

T

A

D

G

G

T

A

A

V

A

A

E

D

V

I

L

V

A

A

K

K

V

L

E

E

F

F

N

N

P

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

V

A

A

M

M

I

L

M

Q

D

A

A

A

L

E

E

Q

R

A

G

G

V

L

I

I

D

K

K

P

D

D

S

T

V

I

L

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

V

I

G

A

L

L

A

A

L

M

T

V

C

C

A

A

L

R

G

G

L

Y

R

R

L

C

I

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

L

E

E

R

R

S

M

L

L

Q

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

G

G

R

A

E

D

G

G

M

M

N

S

G

G

A

A

I
|
I

A

A

R

K

A

A

E

E

L

L

V

A

K

K

S

T

T

D

P

Q

G

R

A

Y

L

F

M

V

L

P

Q

Q

Q
|
Q

F
|
F

A

E

N

N

P

P

A

A

N

N

P

P

R

A

A

I

H

H

V

R

R

V

N

T

T

T

A

A

V

E

E

E

I

V

W

W

E

R

H

D

T

T

G

D

G

G

K

K

V

V

D

D

I

I

F

V

V

V

A

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

T

Q

Y

V

I

I

K

K

E

E

R

R

K

K

P

P

S

S

F

A

Q

R

A

F

I

V

A

A

V

V

E

E

P

P

A

A

E

A

S

S

P

P

V

V

L

L

S

S

G

G

Q

Q

P
x
K

G

G

S

P

H

H

P

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F
|
F

V

V

P

P

K

P

V

V

L

L

N

D

L

Q

A

D

M

L

I

V

D

D

E

E

I

I

F

I

Q

T

V

V

N

G

G

N

E

E

K

D

A

A

M

L

E

N

T

V

A

A

R

R

V

L

A

A

R

R

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

T

Y

V

A

A

L

L

E

Q

V

V

A

A

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

V

L

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T71), S72 (= S72), I126 (= I126), Q144 (= Q144), F145 (= F145), K215 (≠ P215), G222 (= G222), A225 (= A225), F227 (= F227)
binding pyridoxal-5'-phosphate: K44 (= K44), N74 (= N74), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G222), S266 (= S266), P293 (= P293), D294 (= D294)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
65% identity, 96% coverage: 1:300/311 of query aligns to 1:300/300 of 2q3cA

query
sites
2q3cA

M

M

T

S

I

I

Y

A

N

E

N

D

I

I

L

T

E

Q

T

L

I

I

G

G

G

R

T

T

P

P

L

L

V

V

R

R

V

L

N

R

R

R

L

V

A

T

A

D

G

G

T

A

A

V

A

A

E

D

V

I

L

V

A

A

K

K

V

L

E

E

F

F

N

N

P

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

V

A

A

M

M

I

L

M

Q

D

A

A

A

L

E

E

Q

R

A

G

G

V

L

I

I

D

K

K

P

D

D

S

T

V

I

L

I

V

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

V

I

G

A

L

L

A

A

L

M

T

V

C

C

A

A

L

R

G

G

L

Y

R

R

L

C

I

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

L

E

E

R

R

S

M

L

L

Q

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

G

G

R

A

E

D

G

G

M
|
M

N

S

G

G

A

A

I

I

A

A

R

K

A

A

E

E

L

L

V

A

K

K

S

T

T

D

P

Q

G

R

A

Y

L

F

M

V

L

P

Q

Q

Q
|
Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

A

A

I

H

H

V

R

R

V

N

T

T

T

A

A

V

E

E

E

I

V

W

W

E

R

H

D

T

T

G

D

G

G

K

K

V

V

D

D

I

I

F

V

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

T

Q

Y

V

I

I

K

K

E

E

R

R

K

K

P

P

S

S

F

A

Q

R

A

F

I

V

A

A

V

V

E

E

P

P

A

A

E

A

S

S

P

P

V

V

L

L

S

S

G

G

Q

Q

P
x
K

G

G

S

P

H

H

P

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

K

P

V

V

L

L

N

D

L

Q

A

D

M

L

I

V

D

D

E

E

I

I

F

I

Q

T

V

V

N

G

G

N

E

E

K

D

A

A

M

L

E

N

T

V

A

A

R

R

V

L

A

A

R

R

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

T

Y

V

A

A

L

L

E

Q

V

V

A

A

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

V

L

V

L

L

P
|
P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding : T71 (= T71), S72 (= S72), G73 (= G73), T75 (= T75), M122 (= M122), Q144 (= Q144), K215 (≠ P215), G222 (= G222), A225 (= A225)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
58% identity, 99% coverage: 1:307/311 of query aligns to 11:318/323 of 4aecA

query
sites
4aecA

M

L

T

N

I

I

Y

A

N

D

N

N

I

V

L

S

E

Q

T

L

I

I

G

G

G

K

T

T

P

P

L

M

V

V

R

Y

V

L

N

N

R

S

L

I

A

A

A

K

G

G

T

C

A

V

A

A

E

N

V

I

L

A

A

A

K

K

V

L

E

E

F

I

F

M

N

E

P

P

G

C

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

Y

A

S

M

M

I

V

M

T

D

D

A

A

L

E

E

Q

R

K

G

G

V

F

I

I

D

S

K

P

-

G

D

K

S

S

V

V

L

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

G

L

L

A

A

L

F

T

I

C

A

A

A

A

S

L

R

G

G

L

Y

R

R

L

L

I

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

Q

M

E

E

R

R

S

V

L

L

Q

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

D

G

P

R

A

E

K

G

G

M

M

N

T

G

G

A

A

I

V

A

Q

R

K

A

A

E

E

L

I

V

L

K

K

S

N

T

T

P

P

G

D

A

A

L

Y

M

M

L

L

Q

Q

F

F

A

D

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H

H

V

Y

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

D

H

D

T

T

G

K

G

G

K

K

V

V

D

D

I

I

F

F

V

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

G

T

R

Y

F

I

I

K

K

E

E

R

K

K

N

P

P

S

K

F

T

Q

Q

A

V

I

I

A

G

V

V

E

E

P

P

A

T

E

E

S

S

P

D

V

I

L

L

S

S

G

G

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

K

V

N

L

L

N

D

L

Q

A

K

M

I

I

M

D

D

E

E

I

V

F

I

Q

A

V

I

N

S

G

S

E

E

K

E

A

A

M

I

E

E

T

T

A

A

R

K

R

Q

V

L

A

A

R

L

E

K

E

E

G

G

M

L

L

M

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

A

Y

A

A

A

L

I

E

K

V

V

A

A

R

K

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

A

V

V

L

V

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

K

Q

A

S

active site: K54 (= K44), S277 (= S266)
binding pyridoxal-5'-phosphate: K54 (= K44), N85 (= N74), I188 (≠ V177), G189 (= G178), T190 (= T179), G191 (= G180), G192 (= G181), T193 (= T182), G233 (= G222), S277 (= S266), P304 (= P293)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
59% identity, 99% coverage: 1:307/311 of query aligns to 73:380/392 of P47999

query
sites
P47999

M

L

T

N

I

I

Y

A

N

D

N

N

I

A

L

A

E

Q

T

L

I

I

G

G

G

K

T

T

P

P

L

M

V

V

R

Y

V

L

N

N

R

N

L

V

A

V

A

K

G

G

T

C

A

V

A

A

E

S

V

V

L

A

A

A

K

K

V

L

E

E

F

I

F

M

N

E

P

P

G

C

S

S

V

V

K

K

D

D

R

R

I

I

G

G

Y

Y

A

S

M

M

I

I

M

T

D

D

A

A

L

E

E

E

R

K

G

G

V

L

I

I

D

T

K

P

-

G

D

K

S

S

V

V

L

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

V

I

G

G

L

L

A

A

L

F

T

I

C

A

A

A

A

S

L

K

G

G

L

Y

R

K

L

L

I

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

Q

L

E

E

R

R

S

V

L

L

Q

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

E

G

P

R

A

E

K

G

G

M

M

N

T

G

G

A

A

I

I

A

Q

R

K

A

A

E

E

L

I

V

L

K

K

S

K

T

T

P

P

G

N

A

S

L

Y

M

M

L

L

Q

Q

F

F

A

D

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H

H

V

Y

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

E

H

D

T

T

G

R

G

G

K

K

V

I

D

D

I

I

F

L

V

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

G

T

R

Y

F

I

I

K

K

E

E

R

R

K

K

P

P

S

E

F

L

Q

K

A

V

I

I

A

G

V

V

E

E

P

P

A

T

E

E

S

S

P

A

V

I

L

L

S

S

G

G

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

K

K

V

N

L

L

N

D

L

L

A

A

M

I

I

V

D

D

E

E

I

Y

F

I

Q

A

V

I

N

S

G

S

E

E

K

E

A

A

M

I

E

E

T

T

A

S

R

K

R

Q

V

L

A

A

R

L

E

Q

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S

S

G

S

G

G

A

A

M

A

Y

A

A

A

L

I

E

Q

V

V

A

A

R

K

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

A

V

V

L

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

Q

L

L

F

F

K

Q

A

S

Sites not aligning to the query:

61 modified: N-acetylalanine

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
59% identity, 98% coverage: 1:306/311 of query aligns to 1:309/318 of 4lmaA

query
sites
4lmaA

M

M

T

R

I

I

Y

A

N

H

N

D

I

V

L

T

E

E

T

L

I

V

G

G

G

R

T

T

P

P

L

L

V

V

R

Q

V

L

N

N

R

R

L

I

-

P

-

Q

A

A

A

E

G

G

T

C

A

L

A

G

E

R

V

I

L

V

A

M

K

K

V

L

E

E

F

G

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

T

A

H

M

M

I

I

M

N

D

S

A

A

L

E

E

K

R

A

G

G

V

L

I

I

D

N

K

P

D

E

-

T

S

T

V

V

L

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

A

L

L

A

A

L

M

T

T

C

A

A

A

L

K

G

G

L

Y

R

R

L

L

I

I

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

L

E

E

R

R

S

A

L

M

L

L

Q

K

A

A

Y

Y

G

G

A

A

E

T

L

L

I

E

L

L

T

T

P

P

G

G

R

S

E

Q

G

G

M

M

N

K

G

G

A

A

I

I

A

L

R

R

A

A

E

Q

L

I

V

V

K

D

S

S

T

I

P

P

G

G

A

A

L

Y

M

M

L

L

Q

Q

F

F

A

R

N

N

P

P

A

S

N

N

P

P

R

E

A

I

H

H

V

R

R

L

N

T

T

T

A

A

V

E

E

E

I

I

W

W

E

Q

H

D

T

T

G

E

G

G

K

Q

V

V

D

D

I

F

F

I

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

T

E

Y

V

I

I

K

K

E

S

R

R

K

K

P

P

S

S

F

F

Q

Q

A

V

I

V

A

A

V

V

E

E

P

P

A

F

E

N

S

S

P

P

V

V

L

I

S

S

G

G

Q

N

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

E

V

V

L

L

N

R

L

T

A

D

M

L

I

I

D

D

E

E

I

V

F

I

Q

T

V

V

N

S

G

D

E

E

K

E

A

A

M

F

E

Q

T

F

A

G

R

R

V

L

A

A

R

K

E

E

G

G

M

L

L

L

V

S

G

G

I

I

S
|
S

G

S

G

G

A

A

A

N

M

L

Y

C

A

A

L

I

E

Q

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

A

E

G

G

K

K

T

L

V

I

V

V

L

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

L

L

M

F

F

K

Q

active site: K46 (= K44), S269 (= S266)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G222), S269 (= S266), P296 (= P293)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
59% identity, 98% coverage: 1:306/311 of query aligns to 1:309/310 of 4lmbA

query
sites
4lmbA

M

M

T

L

I

I

Y

A

N

R

N

D

I

I

L

T

E

Q

T

L

I

V

G

G

G

R

T

T

P

P

L

L

V

V

R

Q

V

L

N

N

R

R

L

I

-

P

-

V

A

A

A

E

G

G

T

V

A

K

A

A

E

R

V

I

L

V

A

V

K

K

V

L

E

E

F

S

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

V

A

S

M

M

I

V

M

E

D

D

A

A

L

E

E

A

R

A

G

G

V

L

I

I

D

H

K

P

D

D

-

K

S

T

V

I

L

L

V

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

V

I

G

A

L

L

A

A

L

M

T

V

C

A

A

A

L

K

G

G

L

Y

R

R

L

L

I

V

L

L

T

T

M

M

P

P

E

E

T

T

M
|
M

S

S

Q

L

E

E

R

R

S

A

L

M

L

L

Q

K

A

A

Y

Y

G

G

A

A

E

Q

L

L

I

E

L

L

T

T

P

P

G

G

R

S

E

Q

G

G

M
|
M

N

E

G

G

A

A

I

I

A

T

R

R

A

A

E

E

L

I

V

V

K

E

S

N

T

T

P

P

G

N

A

A

L

Y

M

S

L

L

Q

Q

Q
|
Q

F
|
F

A

R

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H

H

V

R

R

E

N

T

T

T

A

A

V

E

E

E

I

I

W

W

E

A

H

D

T

T

G

D

G

G

K

L

V

V

D

D

I

I

F

V

V

I

A

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

S

A

T

E

Y

T

I

I

K

K

E

P

R

R

K

R

P

P

S

Q

F

F

Q

Q

A

A

I

I

A

A

V

V

E

E

P

P

A

S

E

N

S

S

P

P

V

V

L

L

S

S

G

G

Q

Q

P

P

G

G

S

P

H

H

P

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

A

V

I

L

F

N

R

L

P

A

E

M

L

I

I

D

D

E

E

I

V

F

I

Q

I

V

V

N

D

G

D

E

T

K

E

A

A

M

F

E

A

T

Y

A

A

R

R

V

L

A

A

R

R

E

Q

E

E

G

G

M

L

L

L

V

S

G

G

I

I

S
|
S

G

A

G

G

A

A

M

L

Y

W

A

A

L

I

E

Q

V

V

A

G

R

K

R

R

P

P

E

E

N

N

A

E

G

D

K

K

T

L

V

I

V

V

V

M

L

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

K

K

active site: K46 (= K44), S269 (= S266)
binding cysteine: K46 (= K44), T74 (= T71), S75 (= S72), N77 (= N74), T78 (= T75), M101 (= M98), M125 (= M122), M125 (= M122), Q147 (= Q144), F148 (= F145), Q224 (= Q221), G225 (= G222), G225 (= G222), I226 (= I223), A228 (= A225)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), V180 (= V177), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G222), S269 (= S266), P296 (= P293)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
59% identity, 98% coverage: 1:306/311 of query aligns to 2:307/310 of 5xoqA

query
sites
5xoqA

M

M

T

P

I

I

Y

F

N

K

N

D

I

N

L

S

E

E

T

S

I

I

G

G

G

R

T

T

P

P

L

L

V

V

R

Q

V

I

N

N

R

R

L

L

A

T

A

A

G

G

T

L

A

S

E

R

V

V

L

L

A

A

K

K

V

I

E

E

F

G

F

R

N

N

P

P

G

A

G

Y

S

S

V

V

K

K

D

C

R

R

I

I

G

G

Y

A

A

A

M

M

I

I

M

W

D

D

A

A

L

E

E

Q

R

S

G

G

V

K

I

L

D

K

K

P

D

G

S

M

V

H

L

V

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

V

I

G

A

L

L

A

A

L

F

T

V

C

C

A

A

L

R

G

G

L

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

I

E

E

R

R

S

M

L

M

L

L

Q

K

A

S

Y

F

G

G

A

A

E

D

L

L

I

V

L

L

T

T

P

P

G

G

R

A

E

D

G

G

M
|
M

N

K

G

G

A

A

I

I

A

S

R

K

A

A

E

E

L

L

V

A

K

-

S

A

T

Q

P

P

G

G

A

W

L

F

M

I

L

P

Q

Q

Q
|
Q

F

F

A

K

N

N

P

P

A

A

N

N

P

P

R

A

A

I

H

H

V

V

R

K

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

N

H

D

T

T

G

E

G

G

K

Q

V

V

D

D

I

V

F

F

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

T

R

Y

F

I

L

K

K

-

H

E

E

R

K

K

K

P

H

S

P

F

V

Q

H

A

V

I

V

A

A

V

V

E

E

P

P

A

A

E

A

S

S

P

P

V

V

L

L

S

A

G

G

Q

P

P

A

G

G

S
x
R

H
|
H

P

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

K

D

V

T

L

F

N

D

L

R

A

S

M

V

I

V

D

D

E

E

I

I

F

L

Q

S

V

V

N

T

G

D

E

D

K

E

A

A

M

I

E

E

T

T

A

A

R

R

R

K

V

L

A

A

R

M

E

E

G

G

M

I

L

S

V

C

G

G

I

I

S

S

G

C

G

G

A

A

M

M

Y

A

A

G

A

A

L

L

E

K

V

V

A

A

R

A

R

R

P

P

E

E

N

F

A

A

G

G

K

K

T

T

V

I

V

V

V

T

L

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

K

E

binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), M123 (= M122), Q144 (= Q144), R218 (≠ S217), H219 (= H218), Q222 (= Q221), G223 (= G222), A226 (= A225)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
57% identity, 98% coverage: 3:307/311 of query aligns to 5:310/322 of P47998

query
sites
P47998

I

I

Y

A

N

K

N

D

I

V

L

T

E

E

T

L

I

I

G

G

G

N

T

T

P

P

L

L

V

V

R

Y

V

L

N

N

R

N

L

V

A

A

A

E

G

G

T

C

A

V

A

G

E

R

V

V

L

A

A

A

K

K

V

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

F

A

S

M

M

I

I

M

S

D

D

A

A

L

E

E

K

R

K

G

G

V

L

I

I

D

K

K

P

-

G

D

E

S

S

V

V

L

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

V

V

G

G

L

L

A

A

L

F

T

T

C

A

A

A

L

K

G

G

L

Y

R

K

L

L

I

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

Q

T

E

E

R

R

S

I

L

I

L

L

Q

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

G

P

R

A

E

K

G

G

M

M

N

K

G

G

A

A

I

I

A

A

R

K

A

A

E

E

L

I

V

L

K

A

S

K

T

T

P

P

G

N

A

G

L

Y

M

M

L

L

Q

Q

Q
|
Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H
|
H

V

Y

R

E

N

T

T

T

A
x
G

V

P

E

E

I

I

W

W

E

K

H

G

T

T

G

G

K

K

V

I

D

D

I

G

F

F

V

V

A

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

T

K

Y

Y

I

L

K

K

E

E

R

Q

K

N

P

A

S

N

F

V

Q

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

L

L

S

S

G

G

Q
x
K

P

P

G

G

S

P

H
|
H

P
x
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

V

V

L

L

N

N

L

V

A

D

M

L

I

I

D

D

E

E

I

V

F

V

Q

Q

V

V

N

S

G

S

E

D

K

E

A

S

M

I

E

D

T

M

A

A

R

R

R

Q

V

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

A

Y

A

A

A

L

I

E

K

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

F

V

V

V

A

L

I

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

K

D

A

A

K46 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T71) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S72) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N74) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T75) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q144) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H154) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A159) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
T182 (= T179) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T182) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ Q214) mutation to A: Impaired interaction with SAT1.
H221 (= H218) mutation to A: Impaired interaction with SAT1.
K222 (≠ P219) mutation to A: Impaired interaction with SAT1.
S269 (= S266) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
57% identity, 98% coverage: 3:307/311 of query aligns to 3:308/320 of 2isqA

query
sites
2isqA

I

I

Y

A

N

K

N

D

I

V

L

T

E

E

T

L

I

I

G

G

G

N

T

T

P

P

L

L

V

V

R

Y

V

L

N

N

R

N

L

V

A

A

A

E

G

G

T

C

A

V

A

G

E

R

V

V

L

A

A

A

K

K

V

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

Y

F

A

S

M

M

I

I

M

S

D

D

A

A

L

E

E

K

R

K

G

G

V

L

I

I

D

K

K

P

-

G

D

E

S

S

V

V

L

L

V

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

V

V

G

G

L

L

A

A

L

F

T

T

C

A

A

A

L

K

G

G

L

Y

R

K

L

L

I

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

Q

T

E

E

R

R

S

I

L

I

L

L

Q

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

G

P

R

A

E

K

G
|
G

M
|
M

N
x
K

G

G

A

A

I

I

A

A

R

K

A

A

E

E

L

I

V

L

K

A

S

K

T

T

P

P

G

N

A

G

L

Y

M

M

L

L

Q

Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H

H

V

Y

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

K

H

G

T

T

G

G

K

K

V

I

D

D

I

G

F

F

V

V

A

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

T

K

Y

Y

I

L

K

K

E

E

R

Q

K

N

P

A

S

N

F

V

Q

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

L

L

S

S

G

G

Q

K

P

P

G
|
G

S
x
P

H
|
H

P

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

V

V

L

L

N

N

L

V

A

D

M

L

I

I

D

D

E

E

I

V

F

V

Q

Q

V

V

N

S

G

S

E

D

K

E

A

S

M

I

E

D

T

M

A

A

R

R

R

Q

V

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

A

Y

A

A

A

L

I

E

K

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

F

V

V

V

A

L

I

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

K

D

A

A

active site: K44 (= K44), S267 (= S266)
binding pyridoxal-5'-phosphate: K44 (= K44), N75 (= N74), G177 (= G176), G179 (= G178), T180 (= T179), G181 (= G180), T183 (= T182), G223 (= G222), S267 (= S266), P294 (= P293)
binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), G122 (= G121), M123 (= M122), K124 (≠ N123), G217 (= G216), P218 (≠ S217), H219 (= H218), Q222 (= Q221), G223 (= G222)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
57% identity, 98% coverage: 3:307/311 of query aligns to 3:308/320 of 1z7yA

query
sites
1z7yA

I

I

Y

A

N

K

N

D

I

V

L

T

E

E

T

L

I

I

G

G

G

N

T

T

P

P

L

L

V

V

R

Y

V

L

N

N

R

N

L

V

A

A

A

E

G

G

T

C

A

V

A

G

E

R

V

V

L

A

A

A

K

K

V

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

I

I

G

G

Y

F

A

S

M

M

I

I

M

S

D

D

A

A

L

E

E

K

R

K

G

G

V

L

I

I

D

K

K

P

-

G

D

E

S

S

V

V

L

L

V

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

V

V

G

G

L

L

A

A

L

F

T

T

C

A

A

A

L

K

G

G

L

Y

R

K

L

L

I

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

Q

T

E

E

R

R

S

I

L

I

L

L

Q

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

G

P

R

A

E

K

G

G

M

M

N

K

G

G

A

A

I

I

A

A

R

K

A

A

E

E

L

I

V

L

K

A

S

K

T

T

P

P

G

N

A

G

L

Y

M

M

L

L

Q

Q

Q
|
Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H

H

V

Y

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

K

H

G

T

T

G

G

K

K

V

I

D

D

I

G

F

F

V

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

T

K

Y

Y

I

L

K

K

E

E

R

Q

K

N

P

A

S

N

F

V

Q

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

L

L

S

S

G

G

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

V

V

L

L

N

N

L

V

A

D

M

L

I

I

D

D

E

E

I

V

F

V

Q

Q

V

V

N

S

G

S

E

D

K

E

A

S

M

I

E

D

T

M

A

A

R

R

R

Q

V

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

M

A

Y

A

A

A

L

I

E

K

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

F

V

V

V

A

L

I

L

F

P
|
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

K

D

A

A

active site: A44 (≠ K44), S267 (= S266)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G73), N75 (= N74), T76 (= T75), Q145 (= Q144), I178 (≠ V177), G179 (= G178), T180 (= T179), G181 (= G180), T183 (= T182), G223 (= G222), S267 (= S266), P294 (= P293), S295 (≠ D294)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
55% identity, 98% coverage: 2:307/311 of query aligns to 2:308/309 of 7n2tA

query
sites
7n2tA

T

T

I

I

Y

A

N

K

N

D

I

V

L

T

E

E

T

L

I

I

G

G

G

N

T

T

P

P

L

L

V

V

R

Y

V

L

N

N

R

R

L

V

A

V

A

D

G

G

T

C

A

V

A

A

E

R

V

V

L

A

A

A

K

K

V

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K

K

D

D

R

R

I

I

G

G

Y

Y

A

S

M

M

I

I

M

S

D

D

A

A

L

E

E

N

R

K

G

G

V

L

I

I

D

T

K

P

-

G

D

E

S

S

V

V

L

L

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

V

I

G

G

L

L

A

A

L

F

T

I

C

A

A

A

L

K

G

G

L

Y

R

R

L

L

I

I

L

I

T

C

M

M

P

P

E

A

T

S

M

M

S

S

Q

L

E

E

R

R

S

T

L

I

L

L

Q

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

D

G

P

R

A

E

R

G

G

M

M

N

K

G

G

A

A

I

V

A

Q

R

K

A

A

E

E

L

I

V

K

K

A

S

K

T

T

P

P

G

N

A

S

L

Y

M

I

L

L

Q

Q

F

F

A

E

N

N

P

P

A

A

N

N

P

P

R

K

A

I

H

H

V

Y

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

R

H

G

T

S

G

G

K

K

V

I

D

D

I

A

F

L

V

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

I

V

T

T

G

G

V

A

S

G

T

K

Y

Y

I

L

K

K

E

E

R

Q

K

N

P

P

S

N

F

I

Q

K

A

L

I

Y

A

G

V

V

E

E

P

P

A

V

E

E

S

S

P

A

V

I

L

L

S

S

G

G

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

V

V

L

L

N

D

L

V

A

N

M

L

I

L

D

D

E

E

I

V

F

I

Q

Q

V

V

N

S

G

S

E

E

K

E

A

S

M

I

E

E

T

T

A

A

R

K

R

L

V

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S

S

G

S

G

G

A

A

M

A

Y

A

A

A

L

I

E
x
R

V

I

A

A

R

K

R
|
R

P

P

E

E

N

N

A

A

G

G

K

K

T

L

V

I

V

V

V

A

L

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

K

E

A

S

binding phosphate ion: R277 (≠ E276), R281 (= R280)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
55% identity, 97% coverage: 3:305/311 of query aligns to 4:312/323 of P0ABK5

query
sites
P0ABK5

I

I

Y

F

N

E

N

D

I

N

L

S

E

L

T

T

I

I

G

G

G

H

T

T

P

P

L

L

V

V

R

R

V

L

N

N

R

R

L

I

A

G

A

N

G

G

T

-

A

-

E

R

V

I

L

L

A

A

K

K

V

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

Y

A

A

N

M

M

I

I

M

W

D

D

A

A

L

E

E

K

R

R

G

G

V

V

I

L

D

K

K

P

D

G

S

V

V

E

L

L

V

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

V

I

G

A

L

L

A

A

L

Y

T

V

C

A

A

A

L

R

G

G

L

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

I

E

E

R

R

S

K

L

L

Q

K

A

A

Y

L

G

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E

G

G

R

A

E

K

G

G

M

M

N

K

G

G

A

A

I

I

A

Q

R

K

A

A

E

E

L

I

V

V

K

A

S

S

T

N

P

P

-

E

G

K

A

Y

L

L

M

L

L

L

Q

Q

F

F

A

S

N

N

P

P

A

A

N

N

P

P

R

E

A

I

H

H

V

E

R

K

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

E

H

D

T

T

G

D

G

G

K

Q

V

V

D

D

I

V

F

F

V

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

S

S

T

R

Y

Y

I

I

K

K

-

G

-

T

E

K

R

G

K

K

P

T

S

D

F

L

Q

I

A

S

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

A

V

N

L

L

N

D

L

L

A

K

M

L

I

V

D

D

E

K

I

V

F

I

Q

G

V

I

N

T

G

N

E

E

K

E

A

A

M

I

E

S

T

T

A

A

R

R

V

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S

S

G

S

G

G

A

A

M

V

Y

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

T

N

V

I

V

V

L

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

K42 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
55% identity, 97% coverage: 3:305/311 of query aligns to 4:312/323 of P0A1E3

query
sites
P0A1E3

I

I

Y

Y

N

E

N

D

I

N

L

S

E

L

T

T

I

I

G

G

G

H

T

T

P

P

L

L

V

V

R

R

V

L

N

N

R

R

L

I

A

G

A

N

G

G

T

-

A

-

E

R

V

I

L

L

A

A

K

K

V

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K

K

D

C

R

R

I

I

G

G

Y

A

A

N

M

M

I

I

M

W

D

D

A

A

L

E

E

K

R

R

G

G

V

V

I

L

D

K

K

P

D

G

S

V

V

E

L

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

A

L

L

A

A

L

Y

T

V

C

A

A

A

L

R

G

G

L

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

I

E

E

R

R

S

K

L

L

Q

K

A

A

Y

L

G

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E

G

G

R

A

E

K

G

G

M

M

N

K

G

G

A

A

I

I

A

Q

R

K

A

A

E

E

L

I

V

V

K

A

S

S

T

D

P

P

G

Q

A

K

-

Y

L

L

M

L

L

L

Q

Q

F

F

A

S

N

N

P

P

A

A

N

N

P

P

R

E

A

I

H

H

V

E

R

K

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

E

H

D

T

T

G

D

G

G

K

Q

V

V

D

D

I

V

F

F

V

I

A

S

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

S

T

T

R

Y

Y

I

I

K

K

-

G

-

T

E

K

R

G

K

K

P

T

S

D

F

L

Q

I

A

T

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

V

N

L

L

N

D

L

L

A

K

M

L

I

I

D

D

E

K

I

V

F

V

Q

G

V

I

N

T

G

N

E

E

K

E

A

A

M

I

E

S

T

T

A

A

R

R

V

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

G

S

G

G

A

A

M

V

Y

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

T

N

V

I

V

V

L

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

N72 (= N74) binding pyridoxal 5'-phosphate
S273 (= S266) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
55% identity, 97% coverage: 3:305/311 of query aligns to 5:313/321 of 6z4nAAA

query
sites
6z4nAAA

I

I

Y

Y

N

E

N

D

I

N

L

S

E

L

T

T

I

I

G

G

G

H

T

T

P

P

L

L

V

V

R

R

V

L

N

N

R

R

L

I

A

G

A

N

G

G

T

-

A

-

E

R

V

I

L

L

A

A

K

K

V

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

Y

A

A

N

M

M

I

I

M

W

D

D

A

A

L

E

E

K

R

R

G

G

V

V

I

L

D

K

K

P

D

G

S

V

V

E

L

L

V

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

V

I

G

A

L

L

A

A

L

Y

T

V

C

A

A

A

L

R

G

G

L

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

I

E

E

R

R

S

K

L

L

Q

K

A

A

Y

L

G

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E

G

G

R

A

E

K

G

G

M

M

N

K

G

G

A

A

I

I

A

Q

R

K

A

A

E

E

L

I

V

V

K

A

S

S

T

D

P

P

G

Q

A

K

-

Y

L

L

M

L

L

L

Q

Q

Q
|
Q

F
|
F

A

S

N

N

P

P

A

A

N

N

P

P

R

E

A

I

H

H

V

E

R

K

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

E

H

D

T

T

G

D

G

G

K

Q

V

V

D

D

I

V

F

F

V

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

T

R

Y

Y

I

I

K

K

-

G

-

T

E

K

R

G

K

K

P

T

S

D

F

L

Q

I

A

T

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

G

V

N

L

L

N

D

L

L

A

K

M

L

I

I

D

D

E

K

I

V

F

V

Q

G

V

I

N

T

G

N

E

E

K

E

A

A

M

I

E

S

T

T

A

A

R

R

V

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

G

S

G

G

A

A

M

V

Y

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

T

N

V

I

V

V

L

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

binding pyridoxal-5'-phosphate: K43 (= K44), N73 (= N74), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G230 (= G222), S274 (= S266), P301 (= P293)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K44), T70 (= T71), G72 (= G73), N73 (= N74), T74 (= T75), Q144 (= Q144), F145 (= F145), Q229 (= Q221), G230 (= G222), I231 (= I223), A233 (= A225)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
54% identity, 97% coverage: 3:305/311 of query aligns to 3:311/322 of 1d6sA

query
sites
1d6sA

I

I

Y

Y

N

E

N

D

I

N

L

S

E

L

T

T

I

I

G

G

G

H

T

T

P

P

L

L

V

V

R

R

V

L

N

N

R

R

L

I

A

G

A

N

G

G

T

-

A

-

E

R

V

I

L

L

A

A

K

K

V

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
x
A

D

C

R

R

I

I

G

G

Y

A

A

N

M

M

I

I

M

W

D

D

A

A

L

E

E

K

R

R

G

G

V

V

I

L

D

K

K

P

D

G

S

V

V

E

L

L

V

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

V

I

G

A

L

L

A

A

L

Y

T

V

C

A

A

A

L

R

G

G

L

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

Q

I

E

E

R

R

S

K

L

L

Q

K

A

A

Y

L

G

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E

G

G

R

A

E

K

G

G

M

M

N

K

G

G

A

A

I

I

A

Q

R

K

A

A

E

E

L

I

V

V

K

A

S

S

T

D

P

P

G

Q

A

K

-

Y

L

L

M

L

L

L

Q

Q

Q
|
Q

F
|
F

A

S

N

N

P

P

A

A

N

N

P

P

R

E

A

I

H

H

V

E

R

K

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

E

H

D

T

T

G

D

G

G

K

Q

V

V

D

D

I

V

F

F

V

I

A

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

T

R

Y

Y

I

I

K

K

-

G

-

T

E

K

R

G

K

K

P

T

S

D

F

L

Q

I

A

T

I

V

A

A

V

V

E

E

P

P

A

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

S

P

H

H

P

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

V

N

L

L

N

D

L

L

A

K

M

L

I

I

D

D

E

K

I

V

F

V

Q

G

V

I

N

T

G

N

E

E

K

E

A

A

M

I

E

S

T

T

A

A

R

R

V

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

G

S

G

G

A

A

M

V

Y

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

T

N

V

I

V

V

L

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

active site: A41 (≠ K44), G228 (= G222)
binding methionine: T68 (= T71), N69 (≠ S72), N71 (= N74), T72 (= T75), Q142 (= Q144), F143 (= F145), G176 (= G178), G228 (= G222)
binding pyridoxal-5'-phosphate: N71 (= N74), G176 (= G178), T177 (= T179), G178 (= G180), T180 (= T182), G228 (= G222), S272 (= S266), P299 (= P293)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
53% identity, 95% coverage: 11:305/311 of query aligns to 17:311/329 of 8b9wA

query
sites
8b9wA

I

I

G

G

G

N

T

T

P

P

L

A

V

V

R

Y

V

L

N

R

R

N

L

M

A

N

A

K

G

-

T

T

A

A

E

T

V

I

L

V

A

L

K

K

V

L

E

E

F

S

F

E

N

N

P

P

G

M

G

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

A

Y

Y

A

A

M

I

I

Y

M

D

D

K

A

A

L

E

E

K

R

E

G

G

V

K

I

I

D

K

K

R

-

G

D

K

S

S

V

V

L

I

V

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

A

L

L

A

A

L

L

T

L

C

G

A

A

A

V

L

R

G

G

L

Y

R

K

L

V

I

I

L

I

T

T

M

M

P

P

E

D

T

S

M

M

S

S

Q

M

E

E

R

R

S

S

L

L

Q

R

A

I

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

G

A

R

S

E

L

G

G

M

M

N

K

G

G

A

A

I

V

A

A

R

M

A

A

E

N

E

R

L

I

V

V

K

S

S

N

T

N

P

P

G

D

A

A

L

V

M

L

L

A

Q

D

Q

Q

F

F

A

C

N

T

P

K

A

Y

N

N

P

A

R

Q

A

I

H

H

V

E

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

R

H

Q

T

T

G

K

G

G

K

H

V

V

D

D

I

C

F

F

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

A

T

R

Y

F

I

L

K

K

E

S

R

V

K

G

P

C

S

N

F

A

Q

N

A

I

I

V

A

A

V

V

E

E

P

P

A

Q

E

E

S

S

P

P

V

V

L

L

S

S

G

G

Q

R

P

P

G

G

S

P

H

H

P

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

K

E

V

V

L

L

N

D

L

R

A

E

M

L

I

V

D

D

E

E

I

I

F

I

Q

Q

V

V

N

S

G

S

E

D

K

K

A

A

M

I

E

E

T

T

A

A

R

Q

R

K

V

L

A

S

R

R

E

M

E

D

G

G

M

I

L

F

V

C

G

G

I

F

S
|
S

G

A

G

G

A

A

A

N

M

V

Y

F

A

A

L

L

E

K

V

I

A

A

R

E

R

R

P

P

E

E

N

M

A

A

G

G

K

K

T

T

V

I

V

V

V

T

L

V

L

I

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

Y

binding pyridoxal-5'-phosphate: K49 (= K44), N80 (= N74), G184 (= G178), T185 (= T179), G186 (= G180), T188 (= T182), G228 (= G222), S272 (= S266), P299 (= P293)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
48% identity, 98% coverage: 2:306/311 of query aligns to 10:315/329 of 3vbeC

query
sites
3vbeC

T

N

I

I

Y

K

N

K

N

H

I

V

L

S

E

Q

T

L

I

I

G

G

G

R

T

T

P

P

L

L

V

V

R

Y

V

L

N

N

R

K

L

V

A

T

A

E

G

G

T

C

A

G

A

A

E

Y

V

V

L

A

A

V

K

K

V

Q

E

E

F

M

N

Q

P

P

G

T

G

A

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

Y

Y

A

A

M

M

I

I

M

T

D

D

A

A

L

E

E

E

R

K

G

N

V

L

I

I

D

T

K

P

-

G

D

K

S

T

V

T

L

L

V

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

V

I

G

S

L

M

A

A

L

F

T

M

C

A

A

A

A

M

L

K

G

G

L

Y

R

K

L

M

I

V

L

L

T

T

M

M

P

P

E

S

T

Y

M

T

S

S

Q

L

E

E

R

R

S

V

L

T

L

M

Q

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

D

G

P

R

A

E

K

G

G

M

M

N

G

G

G

A

T

I

V

A

K

R

K

A

A

E

Y

E

E

L

L

V

L

K

E

S

N

T

T

P

P

G

N

A

A

L

H

M

M

L

L

Q

Q

F

F

A

S

N

N

P

P

A

A

N

N

P

T

R

Q

A

V

H

H

V

F

R

E

N

T

T

T

A

G

V

P

E

E

I

I

W

W

E

E

H

D

T

T

G

N

G

G

K

Q

V

V

D

D

I

I

F

F

V

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

I

V

T

S

G

G

V

V

S

G

T

Q

Y

Y

I

L

K

K

E

S

R

K

K

N

P

P

S

N

F

V

Q

K

A

I

I

Y

A

G

V

V

E
|
E

P

P

A

S

E

E

S
|
S

P

N

V

V

L

L

S

N

G

G

Q

K

P

P

G

G

S

P

H

H

P

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

K

D

V

I

L

L

N

D

L

L

A

D

M

V

I

M

D

E

E

K

I

V

F

L

Q

E

V

V

N

S

G

S

E

E

K

D

A

A

M

V

E

N

T

M

A

A

R

R

R

V

V

L

A

A

R

L

E

K

E

E

G

G

M

L

L

M

V

V

G

G

I

I

S
|
S

G

S

G

G

A

A

A

N

M

T

Y

V

A

A

L

L

E

R

V

L

A

A

R

Q

R

L

P

P

E

E

N

N

A

K

G

G

K

K

T

L

V

I

V

V

V

T

L

V

L

H

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

P

V

L

L

F

F

K

Q

active site: K52 (= K44), S81 (= S72), E212 (= E203), S216 (= S207), S275 (= S266), P302 (= P293)
binding pyridoxal-5'-phosphate: K52 (= K44), N83 (= N74), M184 (≠ A175), G187 (= G178), S188 (≠ T179), G189 (= G180), T191 (= T182), G231 (= G222), S275 (= S266), P302 (= P293)

4jblB Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with methionine (see paper)
48% identity, 98% coverage: 3:306/311 of query aligns to 12:319/335 of 4jblB

query
sites
4jblB

I

I

Y

Y

N

H

N

N

I

I

L

L

E

E

T

T

I

I

G

G

T

T

P

P

L

L

V

V

R

E

V

L

N

H

-

G

-

V

-

T

-

E

-

H

-

P

R

R

L

I

A

K

G

G

T

T

A

-

E

R

V

I

L

L

A

V

K

K

V

L

E

E

F

Y

F

F

N

N

P

P

G

M

G

S

S

S

V

V

K
|
K

D

D

R

R

I

V

G

G

Y

F

A

N

M

I

I

V

M

Y

D

Q

A

A

L

I

E

K

R

D

G

G

V

R

I

L

D

K

K

P

D

G

S

M

V

E

L

I

V

I

E

E

P

S

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

V

I

G

A

L

L

A

C

L

Q

T

A

C

G

A

A

A

V

L

F

G

G

L

Y

R

R

L

V

I

N

L

I

T

A

M

M

P

P

E

S

T

T

M

M

S

S

Q

V

E

E

R

R

R

Q

S

M

L

I

L

M

Q

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

E

G

G

R

K

E

K

G

G

M
|
M

N

P

G

G

A

A

I

I

A

E

R

E

A

V

E

N

E

K

L

M

V

I

K

K

S

E

T

N

P

P

G

G

A

K

L

Y

M

F

L

V

-

A

Q

N

Q
|
Q

F
|
F

A

G

N

N

P

P

A

D

N

N

P

T

R

A

A

A

H

H

V

-

R

H

N

Y

T

T

A

A

V

N

E

E

I

I

W

W

E

E

H

D

T

T

G

D

G

G

K

E

V

V

D

D

I

I

F

V

V

V

A

S

G

A

V

V

G
|
G

T

T

G

S

G

G

T

T

I

V

T

I

G

G

V

V

S

A

T

E

Y

K

I

L

K

K

E

E

R

K

K

K

P

K

S

G

F

I

Q

K

A

I

I

I

A

A

V

V

E

E

P

P

A

E

E

E

S

S

P

A

V

V

L

L

S

E

G

G

G

K

Q

A

P

K

G

G

S

P

H

H

P

G

I

I

Q

Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

D

V

I

L

Y

N

K

L

K

A

E

M

F

I

V

D

D

E

E

I

I

F

I

Q

P

V

I

N

K

G

T

E

Q

K

D

A

A

M

W

E

K

T

M

A

A

R

R

R

A

V

V

A

V

R

K

E

Y

E

D

G

G

M

I

L

M

V

C

G

G

I

M

S
|
S

G

S

G

G

A

A

M

I

Y

L

A

A

A

G

L

L

E

K

V

E

A

A

R

E

R

K

P

P

E

E

N

N

A

E

G

G

K

K

T

T

V

I

V

V

V

I

L

I

L

V

P

P

D

S

T

C

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

D

L

L

F

Y

K

K

active site: K57 (= K44), S279 (= S266)
binding methionine: K57 (= K44), T84 (= T71), S85 (= S72), N87 (= N74), T88 (= T75), M135 (= M122), Q158 (= Q144), F159 (= F145), G191 (= G178), G235 (= G222), I236 (= I223), A238 (= A225)

Query Sequence

>WP_013840816.1 NCBI__GCF_000215085.1:WP_013840816.1
MTIYNNILETIGGTPLVRVNRLAAGTAAEVLAKVEFFNPGGSVKDRIGYAMIMDALERGV
IDKDSVLVEPTSGNTGVGLALTCAALGLRLILTMPETMSQERRSLLQAYGAELILTPGRE
GMNGAIARAEELVKSTPGALMLQQFANPANPRAHVRNTAVEIWEHTGGKVDIFVAGVGTG
GTITGVSTYIKERKPSFQAIAVEPAESPVLSGGQPGSHPIQGIGAGFVPKVLNLAMIDEI
FQVNGEKAMETARRVAREEGMLVGISGGAAMYAALEVARRPENAGKTVVVLLPDTGERYL
STPLFKAEPAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory