SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013840878.1 NCBI__GCF_000215085.1:WP_013840878.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
64% identity, 100% coverage: 1:444/444 of query aligns to 1:444/444 of P12425

query
sites
P12425

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M1 (= M1) modified: Initiator methionine, Removed
G59 (= G59) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R62) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E131) binding Mg(2+)
E134 (= E133) binding Mg(2+)
E189 (= E188) binding Mg(2+)
V190 (= V189) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E195) binding Mg(2+)
G241 (= G240) binding L-glutamate
H245 (= H244) binding Mg(2+)
G302 (= G302) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E304) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P306) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E333) binding Mg(2+)
E424 (= E424) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

4s0rD Structure of gs-tnra complex (see paper)
64% identity, 100% coverage: 1:444/444 of query aligns to 4:447/447 of 4s0rD

query
sites
4s0rD

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active site: D56 (= D53), E135 (= E131), E137 (= E133), E192 (= E188), E199 (= E195), H248 (= H244), R319 (= R316), E336 (= E333), R338 (= R335)
binding glutamine: E137 (= E133), E192 (= E188), R301 (= R298), E307 (= E304)
binding magnesium ion: I66 (= I63), E135 (= E131), E135 (= E131), E199 (= E195), H248 (= H244), H248 (= H244), E336 (= E333), H419 (≠ R416)
binding : F63 (= F60), V64 (= V61), R65 (= R62), I66 (= I63), D161 (= D157), G241 (= G237), V242 (≠ I238), N243 (= N239), G305 (= G302), Y306 (= Y303), Y376 (= Y373), I426 (= I423), M430 (≠ R427)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
66% identity, 98% coverage: 5:441/444 of query aligns to 3:436/439 of 7tdpA

query
sites
7tdpA

T

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H

Y

Y

I

M

A

A

G

G

L

I

M

L

N

K

H

H

A

A

D

R

A

A

I

M

T

A

A

A

I

I

A

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

V

V

A

A

W

W

S

S

G

A

R

S

N

N

R
|
R

S

S

P

P

L

M

I

I

R
|
R

I

I

P

P

A

A

K

S

R

R

G

G

M

L

S

S

T

T

R
|
R

V

V

E
|
E

L

V

R
|
R

S

N

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

C

M

L

L

H

R

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

R

G

Q

M

M

T

A

P

L

P

P

P

A

A

P

T

I

D

D

R

R

N

N

I

I

Y

Y

E

V

M

M

T

S

L

E

A

E

E

E

R

R

Q

I

E

E

L

E

G

G

I

I

S

P

N

S

L

L

P

P

E

A

N

D

L

L

S

K

E

E

A

A

L

L

K

S

V

E

L

L

A

I

A

R

D

S

N

E

V

V

I

I

K

S

E

D

A

A

L

L

G

G

P

D

H

H

I

A

F

L

E

A

R

Y

F

F

I

Y

E

E

A

L

K

K

S

E

I

I

E

E

W

W

D

D

R

M

Y

Y

R

R

M

T

Q

Q

V

V

S

H

S

Q

W

W

E

E

L

R

D

D

E

Q

Y

Y

L

L

binding adenosine-5'-diphosphate: N123 (= N127), G125 (= G129), E127 (= E131), E179 (= E183), D193 (= D197), Y196 (= Y200), N242 (= N246), S244 (= S248), R316 (= R321), R326 (= R331)
binding magnesium ion: E127 (= E131), E127 (= E131), E129 (= E133), E184 (= E188), E191 (= E195), E191 (= E195), H240 (= H244), E328 (= E333)
binding l-methionine-s-sulfoximine phosphate: E127 (= E131), E129 (= E133), E184 (= E188), E191 (= E195), G236 (= G240), H240 (= H244), R293 (= R298), E299 (= E304), R311 (= R316), R330 (= R335)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
64% identity, 100% coverage: 3:444/444 of query aligns to 2:443/443 of 4lnkA

query
sites
4lnkA

K

K

L

Y

T

T

N

R

D

E

D

D

V

I

R

E

Q

K

M

L

C

V

K

K

D

E

L

E

N

N

V

V

K

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

F

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

T

P

V

V

E

S

Q

Q

L

L

D

G

K
|
K

A

A

L

L

D

D

G

N

E

K

M

V

M
|
M

F

F

D
|
D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

S

N

T

T

F

F

V

V

V

I

F

F

P

P

W

W

K

T

P

A

R

E

E

K

G

G

G

K

V

V

A

A

R

R

L

F

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

F

E

G

G

D

D

P

P

R

R

Y

N

A

N

L

L

K

K

R

R

A

I

L

L

A

K

E

E

A

M

E

E

Q

D

M

L

G

G

Y

F

V

S

-

D

M

F

N

N

V

L

G

G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

E

E

K

G

G

R

E

P

P

T

T

T

L

K

E

T

L

H

N

D

D

K

K

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V
|
V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F
|
F

K

K

Y
|
Y

A

A

D

G

A

A

L

V

D

R

M

S

A

C

D

D

K

D

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N
|
N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

C

N
|
N

Q

L

S
|
S

L

L

I

F

T

K

L

-

D

N

G

G

K

V

N

N

A

A

F

F

Y

F

D

D

P

E

D

N

A

A

P

D

M

L

G

Q

L

L

S

S

K

E

V

T

C

A

M

K

N

H

Y

F

I

I

A

A

G

G

L

I

M

V

N

K

H

H

A

A

D

T

A

S

I

F

T

T

A

A

I

V

A

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

V

V

A

A

W

W

S

S

G

A

R

Q

N

N

R
|
R

S

S

P

P

L

L

I

I

R

R

I

I

P

P

A

A

K
x
S

R

R

G

G

M

I

S
|
S

T

T

R
|
R

V

V

E
|
E

L

V

R
|
R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

C

L

L

L

H

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

N

G

K

M

L

T

E

P

A

P

P

P

A

A

P

T

I

D

D

R

R

N

N

I

I

Y

Y

E

V

M

M

T

S

L

K

A

E

E

E

R

R

Q

M

E

E

L

N

G

G

I

I

S

V

N

D

L

L

P

P

E

A

N

T

L

L

S

A

E

E

A

A

L

L

K

E

V

E

L

F

A

K

A

S

D

N

N

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

I

L

F

F

E

E

R

H

F

F

I

I

E

E

A

A

K

K

S

E

I

I

E

E

W

W

D

D

R

M

Y

F

R

R

M

T

Q

Q

V

V

S

H

S

P

W

W

E

E

L

R

D

E

E

Q

Y

Y

L

M

E

S

K

Q

F

Y

active site: D52 (= D53), E131 (= E131), E133 (= E133), E188 (= E188), E195 (= E195), H244 (= H244), R315 (= R316), E332 (= E333), R334 (= R335)
binding adenosine-5'-diphosphate: K43 (= K44), M50 (= M51), F198 (= F198), Y200 (= Y200), N246 (= N246), S248 (= S248), S324 (≠ K325), S328 (= S329), R330 (= R331)
binding glutamic acid: E133 (= E133), E188 (= E188), V189 (= V189), N239 (= N239), G240 (= G240), G242 (= G242), E303 (= E304)
binding magnesium ion: E131 (= E131), E188 (= E188), E195 (= E195), H244 (= H244), E332 (= E333)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
64% identity, 100% coverage: 3:444/444 of query aligns to 2:443/443 of 4lniA

query
sites
4lniA

K

K

L

Y

T

T

N

R

D

E

D

D

V

I

R

E

Q

K

M

L

C

V

K

K

D

E

L

E

N

N

V

V

K

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

F

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

T

P

V

V

E

S

Q

Q

L

L

D

G

K

K

A

A

L

L

D

D

G

N

E

K

M

V

M

M

F

F

D
|
D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

S

N

T

T

F

F

V

V

V

I

F

F

P

P

W

W

K

T

P

A

R

E

E

K

G

G

G

K

V

V

A

A

R

R

L

F

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

F

E

G

G

D

D

P

P

R

R

Y

N

A

N

L

L

K

K

R

R

A

I

L

L

A

K

E

E

A

M

E

E

Q

D

M

L

G

G

Y

F

V

S

-

D

M

F

N

N

V

L

G

G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

E

E

K

G

G

R

E

P

P

T

T

T

L

K

E

T

L

H

N

D

D

K

K

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F

F

K

K

Y
|
Y

A

A

D

G

A

A

L

V

D

R

M

S

A

C

D

D

K

D

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

N
|
N

Q

L

S
|
S

L

L

I

F

T

K

L

-

D

N

G

G

K

V

N

N

A

A

F

F

Y

F

D

D

P

E

D

N

A

A

P

D

M

L

G

Q

L

L

S

S

K

E

V

T

C

A

M

K

N

H

Y

F

I

I

A

A

G

G

L

I

M

V

N

K

H

H

A

A

D

T

A

S

I

F

T

T

A

A

I

V

A

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

V

V

A

A

W

W

S

S

G

A

R

Q

N

N

R
|
R

S

S

P

P

L

L

I

I

R
|
R

I

I

P

P

A

A

K

S

R

R

G

G

M

I

S

S

T

T

R
|
R

V

V

E
|
E

L

V

R
|
R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

C

L

L

L

H

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

N

G

K

M

L

T

E

P

A

P

P

P

A

A

P

T

I

D

D

R

R

N

N

I

I

Y

Y

E

V

M

M

T

S

L

K

A

E

E

E

R

R

Q

M

E

E

L

N

G

G

I

I

S

V

N

D

L

L

P

P

E

A

N

T

L

L

S

A

E

E

A

A

L

L

K

E

V

E

L

F

A

K

A

S

D

N

N

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

I

L

F

F

E

E

R

H

F

F

I

I

E

E

A

A

K

K

S

E

I

I

E

E

W

W

D

D

R

M

Y

F

R

R

M

T

Q

Q

V

V

S

H

S

P

W

W

E

E

L

R

D

E

E

Q

Y

Y

L

M

E

S

K

Q

F

Y

active site: D52 (= D53), E131 (= E131), E133 (= E133), E188 (= E188), E195 (= E195), H244 (= H244), R315 (= R316), E332 (= E333), R334 (= R335)
binding adenosine-5'-diphosphate: E131 (= E131), E183 (= E183), D197 (= D197), Y200 (= Y200), N246 (= N246), S248 (= S248), R320 (= R321), R330 (= R331)
binding magnesium ion: E131 (= E131), E131 (= E131), E133 (= E133), E188 (= E188), E195 (= E195), E195 (= E195), H244 (= H244), E332 (= E333)
binding l-methionine-s-sulfoximine phosphate: E133 (= E133), E188 (= E188), H244 (= H244), R297 (= R298), E303 (= E304), R315 (= R316), R334 (= R335)

7tfaB Glutamine synthetase (see paper)
66% identity, 98% coverage: 5:441/444 of query aligns to 3:438/441 of 7tfaB

query
sites
7tfaB

T

T

N

R

D

E

D

D

V

I

R

I

Q

R

M

I

C

A

K

E

D

E

L

E

N

N

V

V

K

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

L

F

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

T

P

V

V

E

S

Q

Q

L

L

D

E

K

K

A

A

L

L

D

D

G

N

E

K

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
x
Y

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

S

D

T

T

F

W

V

V

F

F

P

P

W

W

K

V

P

T

R

S

E

D

G

R

G

-

V

V

A

A

R

R

L

L

I

I

C

C

D

D

I

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

F

A

G

G

D

D

P

P

R

R

Y

G

A

I

L

L

K

K

R

R

A

V

L

L

A

K

E

E

A

A

E

E

Q

E

M

L

G

G

Y

Y

V

T

-

S

M

M

N

N

V

V

G

G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

T

D

D

N

E

E

K

G

G

R

D

P

P

T

T

K

E

T

L

H

N

D

D

K

Q

A

G

G

G

Y
|
Y

F

F

D

D

M

L

T

A

P

P

I

M

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

E

M

I

V

V

L

L

T

K

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V
|
V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

A

A

D

D

A

A

L

V

D

K

M

A

A

A

D

D

K

Q

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

C

N

N

Q

Q

S

S

L

L

I

F

T

K

L

-

D

D

G

N

K

E

N

N

A

V

F

F

Y

Y

D

D

P

E

D

T

A

D

P

E

M

L

G

G

L

L

S

S

K

Q

V

T

C

A

M

R

N

H

Y

Y

I

M

A

A

G

G

L

I

M

L

N

K

H

H

A

A

D

R

A

A

I

M

T

A

A

A

I

I

A

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

V

C

Y

Y

V

V

A

A

W

W

S

S

G

A

R

S

N

N

R
|
R

S

S

P

P

L

M

I

I

R

R

I

I

P

P

A

A

K

S

R

R

G

G

M

L

S

S

T

T

R

R

V

V

E
|
E

L

V

R

R

S

N

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

C

M

L

L

H

R

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

R

G

Q

M

M

T

A

P

L

P

P

P

A

A

P

T

I

D

D

R

R

N

N

I

I

Y

Y

E

V

M

M

T

S

L

E

A

E

E

E

R

R

Q

I

E

E

L

E

G

G

I

I

S

P

N

S

L

L

P

P

E

A

N

D

L

L

S

K

E

E

A

A

L

L

K

S

V

E

L

L

A

I

A

R

D

S

N

E

V

V

I

I

K

S

E

D

A

A

L

L

G

G

P

D

H

H

I

A

F

L

E

A

R

Y

F

F

I

Y

E

E

A

L

K

K

S

E

I

I

E

E

W

W

D

D

R
x
M

Y

Y

R

R

M

T

Q

Q

V

V

S

H

S

Q

W

W

E

E

L

R

D

D

E

Q

Y

Y

L

L

binding glutamine: E131 (= E133), Y153 (= Y155), E186 (= E188), G238 (= G240), H242 (= H244), R295 (= R298), E301 (= E304)
binding magnesium ion: E129 (= E131), E131 (= E133), E186 (= E188), E193 (= E195), H242 (= H244), E330 (= E333)
binding : Y58 (≠ F60), R60 (= R62), V187 (= V189), N237 (= N239), G299 (= G302), Y300 (= Y303), R313 (= R316), M424 (≠ R427)

7tdvC Glutamine synthetase (see paper)
62% identity, 99% coverage: 4:444/444 of query aligns to 3:443/443 of 7tdvC

query
sites
7tdvC

L

F

T

T

N

K

D

E

D

D

V

I

R

R

Q

K

M

F

C

V

K

E

D

E

L

E

N

N

V

V

K

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

F

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

V

T

P

V

V

E

S

Q

Q

L

L

D

E

K

K

A

V

L

L

D

D

G

N

E

E

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

H

P

P

D

D

P

L

S

D

T

T

F

W

V

V

V

I

F

F

P

P

W

W

K

T

P

A

R

G

E

Q

G

G

G

K

V

V

A

A

R

R

L

L

I

I

C

C

D

D

I

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

F

E

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

A

V

L

L

A

K

E

E

A

M

E

E

Q

D

M

L

G

G

Y

F

V

T

-

D

M

F

N

N

V

L

G
|
G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

E

E

K

G

G

R

E

P

P

T

T

T

L

K

E

T

L

H

N

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

D

M

M

G

G

F

F

E

D

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

A

A

D

D

A

A

L

V

D

T

M

A

A

C

D

D

K

N

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

F

N
|
N

Q
x
V

S
|
S

L

L

I

-

T

-

L

F

D

K

G

G

K

K

-

E

N

N

A

A

F

F

Y

F

D

D

P

P

D

N

A

T

P

E

M

M

G

G

L

L

S

T

K

E

V

T

C

A

M

Y

N

Q

Y

F

I

T

A

A

G

G

L

V

M

L

N

K

H

N

A

A

D

R

A

G

I

F

T

T

A

A

I

V

A

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

V

I

A

A

W

W

S

S

G

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

I

R
|
R

I

V

P

P

A

S

K

S

R

R

G

G

M

L

S
|
S

T

T

R
|
R

V

I

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

C

I

L

L

H

E

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

N

G

K

M

L

T

K

P

V

P

P

P

E

A

P

T

V

D

N

R

Q

N

N

I

I

Y

Y

E

E

M

M

T

N

L

R

A

E

E

E

R

R

Q

E

E

A

L

V

G

G

I

I

S

Q

N

D

L

L

P

P

E

S

N

T

L

L

S

Y

E

T

A

A

L

L

K

K

V

A

L

M

A

R

A

E

D

N

N

E

V

V

I

I

K

K

E

K

A

A

L

L

G

G

P

N

H

H

I

I

F

Y

E

N

R

Q

F

F

I

I

E

N

A

S

K

K

S

S

I

I

E

E

W

W

D

D

R

Y

Y

Y

R

R

M

T

Q

Q

V

V

S

S

S

E

W

W

E

E

L

R

D

D

E

Q

Y

Y

L

M

E

K

K

Q

F

Y

binding adenosine-5'-diphosphate: G129 (= G129), E131 (= E131), E183 (= E183), D197 (= D197), F198 (= F198), K199 (= K199), Y200 (= Y200), N246 (= N246), V247 (≠ Q247), S248 (= S248), R320 (= R321), S328 (= S329), R330 (= R331)
binding magnesium ion: E131 (= E131), E131 (= E131), E133 (= E133), E188 (= E188), E195 (= E195), E195 (= E195), H244 (= H244), E332 (= E333)
binding l-methionine-s-sulfoximine phosphate: E131 (= E131), E133 (= E133), E188 (= E188), E195 (= E195), G240 (= G240), H244 (= H244), R297 (= R298), E303 (= E304), R315 (= R316)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
63% identity, 100% coverage: 3:444/444 of query aligns to 2:443/443 of 7tf9S

query
sites
7tf9S

K

K

L

Y

T

T

N

K

D

E

D

D

V

I

R

F

Q

R

M

F

C

A

K

D

D

E

L

Q

N

N

V

V

K

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

F

L

G

G

V

I

L

I

K

K

N

N

V

V

A

E

I

I

T

P

V

V

E

S

Q

Q

L

L

D

K

K

K

A

A

L

L

D

D

G

N

E

K

M

I

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

V
|
V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

F

P

P

D

D

P

L

S

D

T

T

F

W

V

V

F

F

P

P

W

W

K

T

P

A

R

E

E

K

G

G

G

K

V

V

A

A

R

R

L

M

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

F

A

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

A

V

L

L

A

K

E

E

A

M

E

E

Q

E

M

L

G

G

Y

F

V

T

-

E

M

F

N

N

V

L

G

G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

E

E

N

G

R

R

R

P

P

T

T

T

L

K

E

T

L

H

N

D

D

K

S

A

G

G

G

Y
|
Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

A

E

D

D

A

A

L

I

D

T

M

A

A

C

D

D

K

S

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

F

N

N

Q

M

S

S

L

L

I

F

T

N

L

E

D

K

G

G

K

-

N

N

A

A

F

F

Y

F

D

D

P

E

D

S

A

G

P

E

M

L

G

E

L

L

S

S

K

Q

V

T

C

A

M

Y

N

H

Y

F

I

L

A

A

G

G

L

M

M

L

N

K

H

H

A

A

D

R

A

G

I

Y

T

T

A

A

I

V

A

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

V

I

A

A

W

W

S

S

G

G

R

K

N

N

R

R

S

S

P

P

L

L

I

V

R

R

I

V

P

P

A

S

K

S

R

R

G

G

M

L

S

S

T

T

R

R

V

L

E
|
E

L

L

R

R

S

S

P

V

D

D

P

P

S

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

C

L

L

L

H

K

A

A

G

G

L

L

D

S

G

G

I

I

K

K

K

D

G

E

M

L

T

T

P

P

P

A

A

P

T

V

D

D

R

R

N

N

I

I

Y

Y

E

G

M

M

T

N

L

E

A

E

E

E

R

R

Q

E

E

A

L

T

G

G

I

I

S

Y

N

D

L

L

P

P

E

E

N

S

L

L

S

G

E

H

A

A

L

L

K

I

V

E

L

L

A

E

A

K

D

N

N

E

V

I

I

I

K

K

E

D

A

G

L

L

G

G

P

E

H

H

I

I

F

F

E

E

R

H

F

F

I

I

E
|
E

A

A

K

K

S

T

I
|
I

E

E

W

C

D

D

R
x
M

Y

F

R

R

M

T

Q

A

V

V

S

H

S

P

W

W

E

E

L

R

D

E

E

Q

Y

Y

L

L

E

E

K

I

F

Y

binding glutamine: E133 (= E133), Y155 (= Y155), E188 (= E188), G240 (= G240), G242 (= G242), R297 (= R298), E303 (= E304)
binding magnesium ion: E131 (= E131), E133 (= E133), E188 (= E188), E195 (= E195), H244 (= H244), E332 (= E333)
binding : F59 (= F60), V60 (= V61), E418 (= E419), I422 (= I423), M426 (≠ R427)

7tenA Glutamine synthetase (see paper)
63% identity, 100% coverage: 3:444/444 of query aligns to 1:442/442 of 7tenA

query
sites
7tenA

K

K

L

Y

T

T

N

K

D

E

D

D

V

I

R

F

Q

R

M

F

C

A

K

D

D

E

L

Q

N

N

V

V

K

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

F

L

G

G

V

I

L

I

K

K

N

N

V

V

A

E

I

I

T

P

V

V

E

S

Q

Q

L

L

D

K

K

K

A

A

L

L

D

D

G

N

E

K

M

I

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

F

P

P

D

D

P

L

S

D

T

T

F

W

V

V

F

F

P

P

W

W

K

T

P

A

R

E

E

K

G

G

G

K

V

V

A

A

R

R

L

M

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

F

A

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

A

V

L

L

A

K

E

E

A

M

E

E

Q

E

M

L

G

G

Y

F

V

T

-

E

M

F

N

N

V

L

G
|
G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

E

E

N

G

R

R

R

P

P

T

T

T

L

K

E

T

L

H

N

D

D

K

S

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

A

E

D

D

A

A

L

I

D

T

M

A

A

C

D

D

K

S

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

F

N
|
N

Q

M

S
|
S

L

L

I

F

T

N

L

E

D

K

G

G

K

-

N

N

A

A

F

F

Y

F

D

D

P

E

D

S

A

G

P

E

M

L

G

E

L

L

S

S

K

Q

V

T

C

A

M

Y

N

H

Y

F

I

L

A

A

G

G

L

M

M

L

N

K

H

H

A

A

D

R

A

G

I

Y

T

T

A

A

I

V

A

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

V

I

A

A

W

W

S

S

G

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

V

R
|
R

I

V

P

P

A

S

K

S

R

R

G

G

M

L

S
|
S

T

T

R
|
R

V

L

E

E

L

L

R
|
R

S

S

P

V

D

D

P

P

S

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

C

L

L

L

H

K

A

A

G

G

L

L

D

S

G

G

I

I

K

K

K

D

G

E

M

L

T

T

P

P

P

A

A

P

T

V

D

D

R

R

N

N

I

I

Y

Y

E

G

M

M

T

N

L

E

A

E

E

E

R

R

Q

E

E

A

L

T

G

G

I

I

S

Y

N

D

L

L

P

P

E

E

N

S

L

L

S

G

E

H

A

A

L

L

K

I

V

E

L

L

A

E

A

K

D

N

N

E

V

I

I

I

K

K

E

D

A

G

L

L

G

G

P

E

H

H

I

I

F

F

E

E

R

H

F

F

I

I

E

E

A

A

K

K

S

T

I

I

E

E

W

C

D

D

R

M

Y

F

R

R

M

T

Q

A

V

V

S

H

S

P

W

W

E

E

L

R

D

E

E

Q

Y

Y

L

L

E

E

K

I

F

Y

binding adenosine-5'-diphosphate: G128 (= G129), E130 (= E131), E182 (= E183), D196 (= D197), F197 (= F198), K198 (= K199), Y199 (= Y200), N245 (= N246), S247 (= S248), R319 (= R321), S327 (= S329), R329 (= R331)
binding l-methionine-s-sulfoximine phosphate: E130 (= E131), E132 (= E133), E187 (= E188), E194 (= E195), N238 (= N239), G239 (= G240), H243 (= H244), R296 (= R298), E302 (= E304), R314 (= R316), R333 (= R335)

7tf6A Glutamine synthetase (see paper)
62% identity, 99% coverage: 4:444/444 of query aligns to 2:438/438 of 7tf6A

query
sites
7tf6A

L

F

T

T

N

K

D

E

D

D

V

I

R

R

Q

K

M

F

C

A

K

E

D

E

L

E

N

N

V

V

K

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

F

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

V

T

P

V

V

E

S

Q

Q

L

L

D

E

K

K

A

V

L

L

D

D

G

N

E

E

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

H

P

P

D

D

P

L

S

D

T

T

F

W

V

V

V

I

F

F

P

P

W

W

K

-

P

-

R

-

E

-

G

G

G

K

V

V

A

A

R

R

L

L

I

I

C

C

D

D

I

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

F

E

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

A

V

L

L

A

K

E

E

A

M

E

E

Q

D

M

L

G

G

Y

F

V

T

-

D

M

F

N

N

V

L

G

G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

E

E

K

G

G

R

E

P

P

T

T

T

L

K

E

T

L

H

N

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

D

M

M

G

G

F

F

E

D

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

A

A

D

D

A

A

L

V

D

T

M

A

A

C

D

D

K

N

I

I

M

Q

T

T

F

F

K

K

L

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G
|
G

I

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

F

N

N

Q

V

S

S

L

L

I

-

T

-

L

F

D

K

G

G

K

K

-

E

N

N

A

A

F

F

Y

F

D

D

P

P

D

N

A

T

P

E

M

M

G

G

L

L

S

T

K

E

V

T

C

A

M

Y

N

Q

Y

F

I

T

A

A

G

G

L

V

M

L

N

K

H

N

A

A

D

R

A

G

I

F

T

T

A

A

I

V

A

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

V

C

Y

Y

V

I

A

A

W

W

S

S

G

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

I

R

R

I

V

P

P

A

S

K

S

R

R

G

G

M

L

S

S

T

T

R

R

V

I

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

C

I

L

L

H

E

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

N

G

K

M

L

T

K

P

V

P

P

P

E

A

P

T

V

D

N

R

Q

N

N

I

I

Y
|
Y

E

E

M

M

T

N

L

R

A

E

E

E

R

R

Q

E

E

A

L

V

G

G

I

I

S

Q

N

D

L

L

P

P

E

S

N

T

L

L

S

Y

E

T

A

A

L

L

K

K

V

A

L

M

A

R

A

E

D

N

N

E

V

V

I

I

K

K

E

K

A

A

L

L

G

G

P

N

H

H

I

I

F

Y

E

N

R

Q

F

F

I

I

E

N

A

S

K

K

S

S

I

I

E

E

W

W

D

D

R
x
Y

Y

Y

R

R

M

T

Q

Q

V

V

S

S

S

E

W

W

E

E

L

R

D

D

E
x
Q

Y

Y

L

M

E

K

K
x
Q

F

Y

binding glutamine: E128 (= E133), E183 (= E188), G235 (= G240), H239 (= H244), R292 (= R298), E298 (= E304)
binding magnesium ion: E126 (= E131), E128 (= E133), E183 (= E188), E190 (= E195), H239 (= H244), E327 (= E333)
binding : F58 (= F60), R60 (= R62), G232 (= G237), N234 (= N239), G296 (= G302), Y297 (= Y303), R310 (= R316), Y367 (= Y373), Y421 (≠ R427), Q433 (≠ E439), Q437 (≠ K443)

8ufjB Glutamine synthetase (see paper)
58% identity, 98% coverage: 5:441/444 of query aligns to 6:441/444 of 8ufjB

query
sites
8ufjB

T

T

N

K

D

E

D

D

V

V

R

L

Q

E

M

A

C

V

K

K

D

E

L

R

N

D

V

V

K

K

F

F

I

I

R

R

L

T

Q

Q

F

F

T

T

D

D

I

T

F

L

G

G

V

I

L

I

K

K

N

S

V

W

A

A

I

I

T

P

V

A

E

E

Q

Q

L

L

D

E

K

E

A

A

L

F

D

E

G

N

E

G

M

V

M

M

F

F

D

D

G

G

S

S

I

I

E

Q

G

G

F

F

V

T

R

R

I

I

E

E

S

S

D

D

M

M

Y

K

L

L

R

A

P

L

D

D

P

P

S

S

T

T

F

F

V

R

V

I

F

L

P

P

W

W

K

R

P

P

R

A

E

T

G

G

G

A

V

V

A

A

R

R

L

I

I

L

C

G

D

D

I

V

Y

Y

N

L

P

P

D

D

G

G

T

N

P

P

F

F

F

K

G

G

D

D

P

P

R

R

Y

Y

A

V

L

L

K

K

R

T

A

A

L

I

A

K

E

E

A

A

E

E

Q

K

M

M

G

G

Y

F

V

S

M

M

N

N

V

V

G

G

P

P

E
|
E

A

L

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

A

E

N

G

G

R

N

P

P

T

T

K

E

T

L

H

T

D

D

K

Q

A

G

G

G

Y

Y

F

F

D

D

M

F

T

A

P

P

I

L

D

D

L

L

G

G

E

Q

N

D

A

V

R

R

D

D

M

I

V

D

L

Y

T

A

L

L

E

E

Q

H

M

M

G

G

F

F

E

Q

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

S

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

R

Y

F

A

G

D

D

A

V

L

L

D

C

M

T

A

A

D

D

K

N

I

V

M

V

T

T

F

F

K

K

L

Y

V

V

R

K

T

S

I

I

A

A

Q

Y

R

H

H

K

G

G

L

Y

H

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H
|
H

V

S

N

N

Q

Q

S

S

L

L

I

F

T

K

L

-

D

D

G

G

K

K

N

N

A

V

F

F

Y

Y

D

D

P

P

D

D

A

T

P

P

M

T

G

K

L

L

S

S

K

Q

V

D

C

A

M

M

N

Y

Y

Y

I

I

A

G

G

G

L

L

M

L

N

K

H

H

A

I

D

R

A

E

I

F

T

T

A

A

I

V

A

T

N

N

P

P

T

V

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

Y

V

I

A

S

W

W

S

S

G

A

R

Q

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

I

P

P

A

A

K

T

R

R

G

G

M

N

S

G

T

T

R

R

V

I

E
|
E

L

L

R

R

S

C

P

P

D

D

P

P

S

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

C

M

L

L

H

R

A

A

G

G

L

L

D

E

G

G

I

I

K

K

K

N

G

K

M

I

T

D

P

P

P

G

P

E

A

P

T

T

D

N

R

V

N

N

I

I

Y

F

E

H

M

L

T

S

L

D

A

K

E

E

R

R

Q

E

E

E

L

R

G

G

I

I

S

R

N

S

L

L

P

P

E

A

N

D

L

L

S

K

E

E

A

A

L

I

K

D

V

E

L

M

A

K

A

G

D

S

N

K

V

F

I

V

K

K

E

E

A

A

L

L

G

G

P

E

H

H

I

V

F

F

E

S

R

H

F

Y

I

L

E

C

A

A

K

K

S

E

I

M

E

E

W

W

D

D

R

E

Y

Y

R

K

M

A

Q

V

V

V

S

H

S

P

W

W

E

E

L

L

D

S

E

R

Y

Y

L

L

binding magnesium ion: E132 (= E131), E134 (= E133), E189 (= E188), E196 (= E195), H245 (= H244), E333 (= E333)

8tfkA Glutamine synthetase (see paper)
58% identity, 98% coverage: 5:441/444 of query aligns to 2:437/440 of 8tfkA

query
sites
8tfkA

T

T

N

K

D

E

D

D

V

V

R

L

Q

E

M

A

C

V

K

K

D

E

L

R

N

D

V

V

K

K

F

F

I

I

R

R

L

T

Q

Q

F

F

T

T

D

D

I

T

F

L

G

G

V

I

L

I

K

K

N

S

V

W

A

A

I

I

T

P

V

A

E

E

Q

Q

L

L

D

E

K

E

A

A

L

F

D

E

G

N

E

G

M

V

M

M

F

F

D

D

G

G

S

S

I

I

E

Q

G

G

F

F

V

T

R

R

I

I

E

E

S

S

D

D

M

M

Y

K

L

L

R

A

P

L

D

D

P

P

S

S

T

T

F

F

V

R

V

I

F

L

P

P

W

W

K

R

P

P

R

A

E

T

G

G

G

A

V

V

A

A

R

R

L

I

I

L

C

G

D

D

I

V

Y

Y

N

L

P

P

D

D

G

G

T

N

P

P

F

F

F

K

G

G

D

D

P

P

R

R

Y

Y

A

V

L

L

K

K

R

T

A

A

L

I

A

K

E

E

A

A

E

E

Q

K

M

M

G

G

Y

F

V

S

M

M

N

N

V

V

G

G

P

P

E
|
E

A

L

E
|
E

F

F

L

L

F

F

H

K

V

L

D

D

N

A

E

N

G

G

R

N

P

P

T

T

K

E

T

L

H

T

D

D

K

Q

A

G

G

G

Y

Y

F

F

D

D

M

F

T

A

P

P

I

L

D

D

L

R

G

A

E

Q

N

D

A

V

R

R

D

D

M

I

V

D

L

Y

T

A

L

L

E

E

Q

H

M

M

G

G

F

F

E

Q

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

S

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K

R

Y
x
F

A

G

D

D

A

V

L

L

D

C

M

T

A

A

D

D

K

N

I

V

M

V

T

T

F

F

K

K

L

Y

V

V

R

K

T

S

I

I

A

A

Q

Y

R

H

H

K

G

G

L

Y

H

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

V

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

S

N
|
N

Q

Q

S

S

L

L

I

F

T

K

L

-

D

D

G

G

K

K

N

N

A

V

F

F

Y

Y

D

D

P

P

D

D

A

T

P

P

M

T

G

K

L

L

S

S

K

Q

V

D

C

A

M

M

N

Y

Y

Y

I

I

A

G

G

G

L

L

M

L

N

K

H

H

A

I

D

R

A

E

I

F

T

T

A

A

I

V

A

T

N

N

P

P

T

V

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E
|
E

A

A

P

P

V

V

Y

Y

V

I

A

S

W

W

S

S

G

A

R

Q

N

N

R
|
R

S

S

P

S

L

L

I

I

R
|
R

I

I

P

P

A

A

K

T

R

R

G

G

M

N

S
x
G

T

T

R
|
R

V

I

E
|
E

L

L

R
|
R

S

C

P

P

D

D

P

P

S

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

C

M

L

L

H

R

A

A

G

G

L

L

D

E

G

G

I

I

K

K

K

N

G

K

M

I

T

D

P

P

P

G

P

E

A

P

T

T

D

N

R

V

N

N

I

I

Y

F

E

H

M

L

T

S

L

D

A

K

E

E

R

R

Q

E

E

E

L

R

G

G

I

I

S

R

N

S

L

L

P

P

E

A

N

D

L

L

S

K

E

E

A

A

L

I

K

D

V

E

L

M

A

K

A

G

D

S

N

K

V

F

I

V

K

K

E

E

A

A

L

L

G

G

P

E

H

H

I

V

F

F

E

S

R

H

F

Y

I

L

E

C

A

A

K

K

S

E

I

M

E

E

W

W

D

D

R

E

Y

Y

R

K

M

A

Q

V

V

V

S

H

S

P

W

W

E

E

L

L

D

S

E

R

Y

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E131), D194 (= D197), F195 (= F198), F197 (≠ Y200), N243 (= N246), R312 (= R316), R317 (= R321), G325 (≠ S329), R327 (= R331)
binding magnesium ion: E128 (= E131), E128 (= E131), E130 (= E133), E185 (= E188), E192 (= E195), E192 (= E195), H241 (= H244), E329 (= E333)
binding l-methionine-s-sulfoximine phosphate: E128 (= E131), E130 (= E133), E185 (= E188), E192 (= E195), G237 (= G240), H241 (= H244), R294 (= R298), E300 (= E304), R312 (= R316), R331 (= R335)

8ooxB Glutamine synthetase (see paper)
59% identity, 99% coverage: 7:444/444 of query aligns to 4:438/438 of 8ooxB

query
sites
8ooxB

D

D

D

E

V

I

R

F

Q

R

M

I

C

V

K

E

D

E

L

K

N

N

V

V

K

R

F

F

I

V

R

R

L

L

Q

Q

F

F

T

V

D

D

I

V

F

Q

G

G

V

I

L

P

K

K

N

N

V

V

A

A

I

I

T

P

V

V

E

G

Q

Q

L

L

D

E

K

K

A

A

L

L

D

G

G

P

E

G

M

I

M

H

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

V

L

L

R

R

P

P

D

D

P

P

S

D

T

T

F

F

V

R

V

V

F

L

P

P

W

W

K

S

P

G

R

N

E

T

G

A

G

E

V

-

A

A

R

R

L

L

I

I

C

C

D

D

I

I

Y

E

N

L

P

P

D

D

G

G

T

K

P

P

F

F

F

M

G

G

D

C

P

P

R

R

Y

Q

A

V

L

L

K

K

R

K

A

N

L

M

A

E

E

E

A

A

E

A

Q

K

M

L

G

G

Y

Y

V

V

M

M

N

N

V

T

G

G

P

P

E

E

A

M

E
|
E

F

F

L

L

F

F

H

K

V

-

D

R

N

Q

E

D

G

G

R

M

P

P

T

T

T

N

K

I

T

P

H

Q

D

D

K

R

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

I

D

D

L

L

G

A

E

E

N

E

A

I

R

K

R

R

D

E

M

I

V

V

L

L

T

V

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

V

E

E

A

A

S

A

H

H

E
|
E

V

V

A

A

P

F

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

A

D

D

N

A

A

L

L

D

A

M

T

A

A

D

D

K

N

I

V

M

I

T

T

F

L

K

K

L

Y

V

V

V

A

R

K

T

T

I

L

A

A

Q

L

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

I

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H

H

V

T

N

N

Q

T

S

S

L

L

I

F

T

K

L

-

D

D

G

G

K

K

N

N

A

A

F

F

Y

Y

D

D

P

P

D

D

A

A

P

P

M

D

G

Q

L

I

S

S

K

D

V

T

C

L

M

R

N

Y

Y

F

I

V

A

G

G

G

L

V

M

L

N

K

H

H

A

I

D

R

A

A

I

I

T

T

A

A

I

I

A

T

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

Y

V

I

A

T

W

W

S

S

G

G

R

P

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

V

P

P

A

A

K

P

R

R

G

G

M

N

S

S

T

T

R

R

V

I

E

E

L

I

R

R

S

S

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

C

I

L

L

H

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

K

N

G

K

M

I

T

E

P

P

P

E

A

R

T

V

D

E

R

K

N

N

I

I

Y

Y

E

K

M

L

T

T

L

E

A

E

E

E

R

R

Q

E

E

K

L

L

G

G

I

I

S

G

N

M

L

L

P

P

E

G

N

T

L

L

S

K

E

E

A

A

L

I

K

E

V

C

L

F

A

K

A

E

D

D

N

E

V

L

I

L

K

V

E

S

A

A

L

L

G

G

P

E

H

H

I

V

F

S

E

Q

R

S

F

I

I

I

E

N

A

V

K

A

S

M

I

A

E

D

W

W

D

D

R

S

Y

Y

R

R

M

T

Q

Q

V

V

S

H

S

Q

W

W

E

E

L

L

D

D

E

R

Y

Y

L

L

E

Q

K

T

F

Y

binding magnesium ion: E129 (= E133), E183 (= E188), E190 (= E195)

8oozA Glutamine synthetase (see paper)
58% identity, 99% coverage: 7:444/444 of query aligns to 4:430/430 of 8oozA

query
sites
8oozA

D

D

D

E

V

I

R

F

Q

R

M

I

C

V

K

E

D

E

L

K

N

N

V

V

K

R

F

F

I

V

R

R

L

L

Q

Q

F

F

T

V

D

D

I

V

F

Q

G

G

V

I

L

P

K

K

N

N

V

V

A

A

I

I

T

P

V

V

E

G

Q

Q

L

L

D

E

K

K

A

A

L

L

D

G

G

P

E

G

M

I

M

H

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

-

V

-

R

-

I

-

E

-

S

S

D

D

M

M

Y

V

L

L

R

R

P

P

D

D

P

P

S

D

T

T

F

F

V

R

V

V

F

L

P

P

W

W

K

S

P

T

R

A

E

E

G

-

V

-

A

A

R

R

L

L

I

I

C

C

D

D

I

I

Y

E

N

L

P

P

D

D

G

G

T

K

P

P

F

F

F

M

G

G

D

C

P

P

R

R

Y

Q

A

V

L

L

K

K

R

K

A

N

L

M

A

E

E

E

A

A

E

A

Q

K

M

L

G

G

Y

Y

V

V

M

M

N

N

V

T

G
|
G

P

P

E
|
E

A

M

E

E

F

F

L

L

F

F

H

K

V

-

D

R

N

Q

E

D

G

G

R

M

P

P

T

T

T

N

K

I

T

P

H

Q

D

D

K

R

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

I

D

D

L

L

G

A

E

E

N

E

A

I

R

K

R

R

D

E

M

I

V

V

L

L

T

V

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

V

E
|
E

A

A

S

A

H

H

E

E

V

V

A

A

P

F

G

G

Q

Q

H

H

E

E

I

I

D

D

F
|
F

K
|
K

Y
|
Y

A

D

D

N

A

A

L

L

D

A

M

T

A

A

D

D

K

N

I

V

M

I

T

T

F

L

K

K

L

Y

V

V

V

A

R

K

T

T

I

L

A

A

Q

L

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

I

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H
|
H

V

T

N
|
N

Q

T

S
|
S

L

L

I

F

T

K

L

-

D

D

G

G

K

K

N

N

A

A

F

F

Y

Y

D

D

P

P

D

D

A

A

P

P

M

D

G

Q

L

I

S

S

K

D

V

T

C

L

M

R

N

Y

Y

F

I

V

A

G

G

G

L

V

M

L

N

K

H

H

A

I

D

R

A

A

I

I

T

T

A

A

I

I

A

T

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

Y

V

I

A

T

W

W

S

S

G

G

R

P

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

V

P

P

A

A

K

P

R

R

G

G

M

N

S
|
S

T

T

R
|
R

V

I

E
|
E

L

I

R

R

S

S

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

C

I

L

L

H

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

K

N

G

K

M

I

T

E

P

P

P

E

A

R

T

V

D

E

R

K

N

N

I

I

Y

Y

E

K

M

L

T

T

L

E

A

E

E

E

R

R

Q

E

E

K

L

L

G

G

I

I

S

G

N

M

L

L

P

P

E

G

N

T

L

L

S

K

E

E

A

A

L

I

K

E

V

C

L

F

A

K

A

E

D

D

N

E

V

L

I

L

K

V

E

S

A

A

L

L

G

G

P

E

H

H

I

V

F

S

E

Q

R

S

F

I

I

I

E

N

A

V

K

A

S

M

I

A

E

D

W

W

D

D

R

S

Y

Y

R

R

M

T

Q

Q

V

V

S

H

S

Q

W

W

E

E

L

L

D

D

E

R

Y

Y

L

L

E

Q

K

T

F

Y

binding adenosine-5'-triphosphate: G117 (= G129), E170 (= E183), F185 (= F198), K186 (= K199), Y187 (= Y200), N233 (= N246), S235 (= S248), S315 (= S329), R317 (= R331)
binding magnesium ion: E119 (= E131), H231 (= H244), E319 (= E333)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
55% identity, 99% coverage: 5:442/444 of query aligns to 1:445/446 of 8ooqB

query
sites
8ooqB

T

T

N

V

D

E

D

Q

V

V

R

L

Q

E

M

Y

C

V

K

K

D

S

L

N

N

N

V

V

K

K

F
|
F

I

M

R
|
R

L

F

Q

Q

F

F

T

V

D

D

I

I

F

L

G

G

V

V

L

P

K

K

N

N

V

V

A
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A

I

F

T

P

V

I

-

K

-

A

-

G

-

E

-

K

-

G

-

I

E

E

Q

E

L

L

D

R

K

D

A

V

L

L

D

E

G

N

E

G

M

L

M

Y

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

M

L

L

R

K

P

P

D

D

P

L

S

S

T

T

F

F

V

S

V

V

F

L

P

P

W

W

K
x
R

P

P

R

S

E

E

G

K

G

S

V
|
V

A

A

R

R

L

V

I

I

C

C

D

D

I

V

Y

Y

N

T

P

T

D

K

G

G

T

K

P

P

F

F

F

E

G

G

D

D

P

P

R

R

Y

G

A

C

L

L

K

K

R

R

A

V

L

M

A

E

E

E

-

F

A

K

E

K

Q

E

M

F

G

N

Y

G

V

E

M

Y

N

F

V

V

G

G

P

P

E

E

A

P

E
|
E

F

F

L

L

F

L

H

K

V

K

D

D

N

P

E

H

G

N

R

P

P

H

T

K

T

Y

K

I

T

P

H

A

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

E

P

P

I

M

D

D

L

E

G

A

E
x
P

N

D

A

I

R
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R

D

D

M

I

V

V

L

F

T

A

L

L

E

E

Q

N

M

L

G

G

F

F

E

H

I

V

E

E

A

A

S
|
S

H

H

E
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E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
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E

I

V

D

D

F

F

K

K

Y

F

A

D

D

D

A

A

L

L

D

K

M

T

A

A

D

D

K

S

I

V

M

I

T

T

F

F

K

K

L

T

V

T

V

I

R

K

T

T

I

I

A

A

Q

E

R

Q

H

H

G

G

L

L

H

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

F

F

F

G

G

I

M

N

N

G

G

S

S

G

G

M

M

H

H

V

C

N

H

Q

Q

S

S

L

-

I

I

T

W

L

L

D

N

G

G

K

E

N

P

A

S

F

F

Y

Y

D

D

P

E

D

N

A

A

P

P

M

Y

G

Q

L

L

S

S

K

E

V

T

C

C

M

M

N

N

Y

Y

I

V

A

A

G

G

L

I

M

L

N

K

H

H

A

A

D

K

A

A

I

I

T

V

A

A

I

I

A

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

N

V

I

A

A

W

W

S

A

G

N

R

S

N

N

R

R

S

S

P

A

L

I

I

I

R

R

I

V

P

P

A

A

K

A

R

R

G

G

M

K

S

G

T

T

R

R

V

I

E

E

L

F

R

R

S

A

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

A

V

C

M

L

L

H

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

K

N

G

K

M

L

T

D

P

A

P

P

P

E

A

P

T

V

D

E

R

R

N

N

I

I

Y

F

E

A

M

M

T

S

L

E

A

A

E

E

R

K

Q

K

E

E

L

L

G

G

I

I

S

E

N

S

L

V

P

P

E

A

N

N

L

L

S

K

E

A

A

A

L

L

K

D

V

E

L

L

A

E

A

N

D

N

N

D

V

V

I

L

K

K

E

N

A

A

L

L

G

G

P

K

H

H

I

I

F

F

E

E

R

S

F

F

I

L

E

E

A

I

K

K

S

N

I

A

E

E

W

W

D

D

R

S

Y

F

R

R

M

T

Q

S

V

V

S

T

S

D

W

W

E

E

L

T

D

T

E

A

Y

Y

L

L

E

K

binding 2-oxoglutaric acid: F16 (= F20), R18 (= R22), A32 (= A36), R86 (≠ K83), V92 (= V89), P169 (≠ E165), R172 (= R168), R173 (= R169), S189 (= S185)
binding magnesium ion: E137 (= E133), E192 (= E188), E199 (= E195)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
55% identity, 99% coverage: 5:442/444 of query aligns to 2:446/447 of 8oooA

query
sites
8oooA

T

T

N

V

D

E

D

Q

V

V

R

L

Q

E

M

Y

C

V

K

K

D

S

L

N

N

N

V

V

K

K

F
|
F

I

M

R
|
R

L

F

Q

Q

F

F

T

V

D

D

I

I

F

L

G

G

V

V

L

P

K

K

N

N

V

V

A
|
A

I

F

T

P

V

I

-

K

-

A

-

G

-

E

-

K

-

G

-

I

E

E

Q

E

L

L

D

R

K

D

A

V

L

L

D

E

G

N

E

G

M

L

M

Y

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

M

L

L

R

K

P

P

D

D

P

L

S

S

T

T

F

F

V

S

V

V

F

L

P

P

W

W

K
x
R

P

P

R

S

E

E

G

K

G

S

V
|
V

A

A

R

R

L

V

I

I

C

C

D

D

I

V

Y

Y

N

T

P

T

D

K

G

G

T

K

P

P

F

F

F

E

G

G

D

D

P

P

R

R

Y

G

A

C

L

L

K

K

R

R

A

V

L

M

A

E

E

E

-

F

A

K

E

K

Q

E

M

F

G

N

Y

G

V

E

M

Y

N

F

V

V

G

G

P

P

E
|
E

A

P

E

E

F

F

L

L

F

L

H

K

V

K

D

D

N

P

E

H

G

N

R

P

P

H

T

K

T

Y

K

I

T

P

H

A

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

E

P

P

I

M

D

D

L

E

G

A

E
x
P

N

D

A

I

R
|
R

D

D

M

I

V

V

L

F

T

A

L

L

E

E

Q

N

M

L

G

G

F

F

E

H

I

V

E
|
E

A

A

S
|
S

H

H

E

E

V

V

A

A

P

P

G

G

Q

Q

H

H

E

E

I

V

D

D

F
|
F

K
|
K

Y
x
F

A

D

D

D

A

A

L

L

D

K

M

T

A

A

D

D

K

S

I

V

M

I

T

T

F

F

K

K

L

T

V

T

V

I

R

K

T

T

I

I

A

A

Q

E

R

Q

H

H

G

G

L

L

H

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

F

F

F

G

G

I

M

N

N

G

G

S

S

G

G

M

M

H

H

V

C

N
x
H

Q

Q

S
|
S

L

-

I

I

T

W

L

L

D

N

G

G

K

E

N

P

A

S

F

F

Y

Y

D

D

P

E

D

N

A

A

P

P

M

Y

G

Q

L

L

S

S

K

E

V

T

C

C

M

M

N

N

Y

Y

I

V

A

A

G

G

L

I

M

L

N

K

H

H

A

A

D

K

A

A

I

I

T

V

A

A

I

I

A

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

N

V

I

A

A

W

W

S

A

G

N

R

S

N

N

R

R

S

S

P

A

L

I

I

I

R
|
R

I

V

P

P

A

A

K

A

R

R

G

G

M

K

S

G

T

T

R
|
R

V

I

E

E

L

F

R

R

S

A

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

A

V

C

M

L

L

H

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

K

N

G

K

M

L

T

D

P

A

P

P

P

E

A

P

T

V

D

E

R

R

N

N

I

I

Y

F

E

A

M

M

T

S

L

E

A

A

E

E

R

K

Q

K

E

E

L

L

G

G

I

I

S

E

N

S

L

V

P

P

E

A

N

N

L

L

S

K

E

A

A

A

L

L

K

D

V

E

L

L

A

E

A

N

D

N

N

D

V

V

I

L

K

K

E

N

A

A

L

L

G

G

P

K

H

H

I

I

F

F

E

E

R

S

F

F

I

L

E

E

A

I

K

K

S

N

I

A

E

E

W

W

D

D

R

S

Y

F

R

R

M

T

Q

S

V

V

S

T

S

D

W

W

E

E

L

T

D

T

E

A

Y

Y

L

L

E

K

binding 2-oxoglutaric acid: F17 (= F20), R19 (= R22), A33 (= A36), R87 (≠ K83), V93 (= V89), P170 (≠ E165), R173 (= R168), R174 (= R169), S190 (= S185)
binding adenosine-5'-triphosphate: E136 (= E131), E188 (= E183), F203 (= F198), K204 (= K199), F205 (≠ Y200), H251 (≠ N246), S253 (= S248), R325 (= R321), R335 (= R331)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
47% identity, 98% coverage: 9:441/444 of query aligns to 8:444/446 of A0R083

query
sites
A0R083

V

V

R

L

Q

R

M

T

C

L

K

E

D

E

L

R

N

D

V

I

K

R

F

F

I

V

R

R

L

L

Q

W

F

F

T

T

D

D

I

V

F

L

G

G

V

Y

L

L

K

K

N

S

V

V

A

A

I

I

T

A

V

P

E

A

Q

E

L

L

D

E

K

G

A

A

L

F

D

E

G

E

E

G

M

I

M

G

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

A

R

R

I

V

E

F

E

E

S

S

D

D

M

T

Y

V

L

A

R

R

P

P

D

D

P

P

S

S

T

T

F

F

V

Q

V

V

F

L

P

P

W

W

K

K

P

T

R

S

E

D

G

G

G

N

-

H

-

Y

V

S

A

A

R

R

L

M

I

F

C

C

D

D

I

I

Y

T

N

M

P

P

D

D

G

G

T

S

P

P

F

S

F

W

G

A

D

D

P

S

R

R

Y

H

A

V

L

L

K

R

R

R

A

Q

L

L

A

A

E

K

A

A

E

S

Q

D

M

L

G

G

Y

F

V

T

M

C

N

Y

V

V

G

H

P

P

E

E

A

I

E

E

F

F

L

L

F

L

H

K

V

P

D

G

-

P

N

N

E

D

G

G

R

T

P

P

T

P

T

E

K

P

T

A

H

-

D

D

K

N

A

G

G

G

Y

Y

F

F

D

D

M

Q

T

A

P

V

I

H

D

D

L

A

G

A

E

P

N

N

A

F

R

R

D

H

M

A

V

I

L

E

T

A

L

L

E

E

Q

Q

M

M

G

G

F

I

E

S

I

V

E

E

A

F

S

S

H

H

E

E

V

G

A

A

P

P

G

G

Q

Q

H

Q

E

E

I

I

D

D

F

L

K

R

Y

Y

A

A

D

D

A

A

L

L

D

S

M

M

A

A

D

D

K

N

I

V

M

M

T

T

F

F

K

R

L

Y

V

L

V

V

R

K

T

E

I

V

A

A

Q

L

R

A

H

D

G

G

L

V

H

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

V

F

F

A

G

E

I

H

N

P

G

G

S

S

G

A

M

M

H

H

V

T

N

H

Q

M

S

S

L

L

I

F

T

E

L

G

D

D

G

-

K

T

N

N

A

A

F

F

Y

H

D

S

P

P

D

D

A

D

P

P

M

L

G

Q

L

L

S

S

K

D

V

V

C

A

M

K

N

S

Y

F

I

I

A

A

G

G

L

I

M

L

N

E

H

H

A

A

D

N

A

E

I

I

T

S

A

A

I

V

A

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

H

G

G

Y

G

E

E

A

A

P

P

V

T

Y

A

V

A

A

S

W

W

S

G

G

A

R

A

N

N

R

R

S

S

P

A

L

L

I

V

R

R

I

V

P

P

-

M

-

Y

-

T

A

P

K

H

R

K

G

V

M

S

S

S

T

R

R

R

V

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

S

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

C

L

L

L

H

A

A

A

G

G

L

L

D

R

G

G

I

V

K

E

K
|
K

G

G

M

Y

T

V

P

L

P

G

P

P

A

Q

T

A

D

E

R

D

N

N

I

V

Y

W

E

S

M

L

T

T

L

Q

A

E

E

E

R

R

Q

R

E

A

L

M

G

G

I

Y

S

R

N

E

L

L

P

P

E

T

N

S

L

L

S

G

E

N

A

A

L

L

K

E

V

S

L

M

A

E

A

N

D

S

N

E

V

L

I

V

K

A

E

E

A

A

L

L

G

G

P

E

H

H

I

V

F

F

E

D

R

Y

F

F

I

L

E

R

A

N

K

K

S

R

I

S

E

E

W

W

D

E

R

N

Y

Y

R

R

M

S

Q

H

V

V

S

T

S

P

W

Y

E

E

L

L

D

K

E

N

Y

Y

L

L

K363 (= K360) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 98% coverage: 9:441/444 of query aligns to 8:444/446 of P9WN37

query
sites
P9WN37

V

V

R

L

Q

R

M

T

C

L

K

E

D

E

L

R

N

D

V

I

K

R

F

F

I

V

R

R

L

L

Q

W

F

F

T

T

D

D

I

V

F

L

G

G

V

F

L

L

K

K

N

S

V

V

A

A

I

I

T

A

V

P

E

A

Q

E

L

L

D

E

K

G

A

A

L

F

D

E

G

E

E

G

M

I

M

G

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

A

R

R

I

V

E

S

E

E

S

S

D

D

M

T

Y

V

L

A

R

H

P

P

D

D

P

P

S

S

T

T

F

F

V

Q

V

V

F

L

P

P

W

W

K

A

P

T

R

S

E

S

G

G

G

H

-

H

V

S

A

A

R

R

L

M

I

F

C

C

D

D

I

I

Y

T

N

M

P

P

D

D

G

G

T

S

P

P

F

S

F

W

G

A

D

D

P

P

R

R

Y

H

A

V

L

L

K

R

R

R

A

Q

L

L

A

T

E

K

A

A

E

G

Q

E

M

L

G

G

Y

F

V

S

M

C

N

Y

V

V

G

H

P

P

E

E

A

I

E

E

F

F

L

L

F

L

H

K

V

P

D

G

N

P

E

E

G

D

R

G

P

S

T

V

T

P

K

V

T

P

H

V

D

D

K

N

A

A

G

G

Y

Y

F

F

D

D

M

Q

T

A

P

V

I

H

D

D

L

S

G

A

E

L

N

N

A

F

R

R

D

H

M

A

V

I

L

D

T

A

L

L

E

E

Q

F

M

M

G

G

F

I

E

S

I

V

E

E

A

F

S

S

H

H

E

E

V

G

A

A

P

P

G

G

Q

Q

H

Q

E

E

I

I

D

D

F

L

K

R

Y

F

A

A

D

D

A

A

L

L

D

S

M

M

A

A

D

D

K

N

I

V

M

M

T

T

F

F

K

R

L

Y

V

V

V

I

R

K

T

E

I

V

A

A

Q

L

R

E

H

E

G

G

L

A

H

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

V

F

F

G

G

Q

I

H

N

P

G

G

S

S

G

A

M

M

H

H

V

T

N

H

Q

M

S

S

L

L

I

F

T

E

L

G

D

D

G

-

K

V

N

N

A

A

F

F

Y

H

D

S

P

A

D

D

A

D

P

P

M

L

G

Q

L

L

S

S

K

E

V

V

C

G

M

K

N

S

Y

F

I

I

A

A

G

G

L

I

M

L

N

E

H

H

A

A

D

C

A

E

I

I

T

S

A

A

I

V

A

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

Q

G

G

Y

G

E

E

A

A

P

P

V

T

Y

A

V

A

A

S

W

W

S

G

G

A

R

A

N

N

R

R

S

S

P

A

L

L

I

V

R

R

I

V

P

P

-

M

-

Y

-

T

A

P

K

H

R

K

G

T

M

S

S

S

T

R

R

R

V

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

S

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

C

L

L

L

H

A

A

A

G

G

L

L

D

R

G

G

I

V

K

E

K
|
K

G

G

M

Y

T

V

P

L

P

G

P

P

A

Q

T

A

D

E

R

D

N

N

I

V

Y

W

E

D

M

L

T

T

L

P

A

E

E

E

R

R

Q

R

E

A

L

M

G

G

I

Y

S

R

N

E

L

L

P

P

E

S

N

S

L

L

S

D

E

S

A

A

L

L

K

R

V

A

L

M

A

E

A

A

D

S

N

E

V

L

I

V

K

A

E

E

A

A

L

L

G

G

P

E

H

H

I

V

F

F

E

D

R

F

F

F

I

L

E

R

A

N

K

K

S

R

I

T

E

E

W

W

D

A

R

N

Y

Y

R

R

M

S

Q

H

V

V

S

T

S

P

W

Y

E

E

L

L

D

R

E

T

Y

Y

L

L

K363 (= K360) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 98% coverage: 4:440/444 of query aligns to 1:466/469 of P0A9C5

query
sites
P0A9C5

L
x
M

T

S

N

A

D

E

D

H

V

V

R

L

Q

T

M

M

C

L

K

N

D

E

L

H

N

E

V

V

K

K

F

F

I

V

R

D

L

L

Q

R

F

F

T

T

D

D

I

T

F

K

G

G

V

K

L

E

K

Q

N

H

V

V

A

T

I

I

T

P

V

A

E

H

Q

Q

L

V

D

N

K

A

A

E

L

F

D

F

G

E

E

E

-

G

M

K

M

M

F

F

D

D

G

G

S

S

I

I

E

G

G

G

F

W

V

K

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

V

L

L

R

M

P

P

D

D

P

A

S

S

T

T

F

A

V

V

V

I

F

D

P

P

W

F

K

F

P

A

R

D

E

S

G

T

G

L

V

I

A

I

R

R

L

-

I

-

C

C

D

D

I

I

Y

L

N

E

P

P

D

G

G

T

T

L

P

Q

F

G

F

Y

G

D

-

R

D

D

P

P

R

R

Y

S

A

I

L

A

K

K

R

R

A

A

L

E

A

D

E

Y

A

L

E

R

Q

S

M

T

G

G

Y

I

V

A

M

D

N

T

V

V

-

L

-

F

G

G

P

P

E

E

A

P

E

E

F

F

L

L

F

F

-

D

-

I

-

R

-

F

-

G

-

S

-

I

-

S

-

G

-

S

H

H

V

V

-

A

-

I

-

D

D

D

N

I

E

E

G

G

R

A

P

W

T

N

T

S

K

S

T

T

H

Q

D

Y

-

E

-

G

-

N

-

K

-

G

-

H

-

R

-

P

-

A

-

V

K

K

A

G

G

G

Y

Y

F

F

D

P

M

V

T

P

P

P

I

V

D

D

L

S

G

A

E

Q

N

D

A

I

R

R

R

S

D

E

M

M

V

C

L

L

T

V

L

M

E

E

Q

Q

M

M

G

G

F

L

E

V

I

V

E

E

A

A

S

H

H

H

E

E

V

V

A

A

-

T

P

A

G

G

Q

Q

H

N

E

E

I

V

D

A

F

T

K

R

Y

F

A

N

D

T

A

M

L

T

D

K

M

K

A

A

D

D

K

E

I

I

M

Q

T

I

F

Y

K

K

L

Y

V

V

R

H

T

N

I

V

A

A

Q

H

R

R

H

F

G

G

L

K

H

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

F

F

G

G

I

D

N

N

G

G

S

S

G

G

M

M

H

H

V

C

N

H

Q

M

S

S

L

L

I

-

T

S

L

K

D

N

G

G

K

V

N

N

A

L

F

F

Y

A

D

G

P

-

D

D

A

K

P

Y

M

A

G

G

L

L

S

S

K

E

V

Q

C

A

M

L

N

Y

Y

Y

I

I

A

G

G

G

L

V

M

I

N

K

H

H

A

A

D

K

A

A

I

I

T

N

A

A

I

L

A

A

N

N

P

P

T

T

V

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

M

V

L

A

A

W

Y

S

S

G

A

R

R

N

N

R

R

S

S

P

A

L

S

I

I

R

R

I

I

P

P

A

V

K

V

R

S

G

S

-

P

M

K

S

A

T

R

R

R

V

I

E

E

L

V

R

R

S

F

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

C

L

F

A

A

C

L

L

L

H

M

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

N

G

K

M

I

T

H

P

P

P

G

P

E

A

A

T

M

D

D

R

K

N

N

I

L

Y
|
Y

E

D

M

L

T

P

L

P

A

E

E

E

R

A

Q

K

E

E

L

-

G

-

I

I

S

P

N

Q

L

V

P

A

E

G

N

S

L

L

S

E

E

E

A

A

L

L

K

N

V

E

L

L

A

D

A

L

D

D

N

R

V

E

I

F

K

L

E

K

A

A

L

G

G

G

-

V

-

F

-

T

P

D

H

E

I

A

F

I

E

D

R

A

F

Y

I

I

E

A

A

L

K

R

S

R

I

E

E

E

W

D

D

D

R

R

Y

V

R

R

M

M

Q

T

V

P

S

H

S

P

W

V

E

E

L

F

D

E

E

L

Y

Y

M1 (≠ L4) modified: Initiator methionine, Removed
Y398 (= Y373) modified: O-AMP-tyrosine

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
42% identity, 98% coverage: 4:440/444 of query aligns to 1:466/469 of P0A1P6

query
sites
P0A1P6

L

M

T

S

N

A

D

E

D

H

V

V

R

L

Q

T

M

M

C

L

K

N

D

E

L

H

N

E

V

V

K

K

F

F

I

V

R

D

L

L

Q

R

F

F

T

T

D

D

I

T

F

K

G

G

V

K

L

E

K

Q

N

H

V

V

A

T

I

I

T

P

V

A

E

H

Q

Q

L

V

D

N

K

A

A

E

L

F

D

F

G

E

E

E

-

G

M

K

M

M

F

F

D

D

G

G

S

S

I

I

E

G

G

G

F

W

V

K

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

V

L

L

R

M

P

P

D

D

P

A

S

S

T

T

F

A

V

V

V

I

F

D

P

P

W

F

K

F

P

A

R

D

E

S

G

T

G

L

V

I

A

I

R

R

L

-

I

-

C

C

D

D

I

I

Y

L

N

E

P

P

D

G

G

T

T

L

P

Q

F

G

F

Y

G

D

-

R

D

D

P

P

R

R

Y

S

A

I

L

A

K

K

R

R

A

A

L

E

A

D

E

Y

A

L

E

R

Q

A

M

T

G

G

Y

I

V

A

M

D

N

T

V

V

-

L

-

F

G

G

P

P

E
|
E

A

P

E
|
E

F

F

L

L

F

F

-

D

-

I

-

R

-

F

-

G

-

A

-

S

-

I

-

S

-

G

-

S

H

H

V

V

-

A

-

I

-

D

D

D

N

I

E

E

G

G

R

A

P

W

T

N

T

S

K

S

T

T

H

K

D

Y

-

E

-

G

-

N

-

K

-

G

-

H

-

R

-

P

-

G

-

V

K

K

A

G

G

G

Y

Y

F

F

D

P

M

V

T

P

P

P

I

V

D

D

L

S

G

A

E

Q

N

D

A

I

R

R

R

S

D

E

M

M

V

C

L

L

T

V

L

M

E

E

Q

Q

M

M

G

G

F

L

E

V

I

V

E
|
E

A

A

S

H

H

H

E
|
E

V

V

A

A

-

T

P

A

G

G

Q

Q

H

N

E
|
E

I

V

D

A

F

T

K

R

Y

F

A

N

D

T

A

M

L

T

D

K

M

K

A

A

D

D

K

E

I

I

M

Q

T

I

F

Y

K

K

L

Y

V

V

R

H

T

N

I

V

A

A

Q

H

R

R

H

F

G

G

L

K

H

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

F

F

G

G

I

D

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

C

N
x
H

Q
x
M

S
|
S

L

L

I

A

T

K

L

-

D

N

G

G

K

T

N

N

A

L

F

F

Y

-

D

S

P

G

D

D

A

K

P

Y

M

A

G

G

L

L

S

S

K

E

V

Q

C

A

M

L

N

Y

Y

Y

I

I

A

G

G

G

L

V

M

I

N

K

H

H

A

A

D

K

A

A

I

I

T

N

A

A

I

L

A

A

N

N

P

P

T

T

V

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

A

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

M

V

L

A

A

W

Y

S

S

G

A

R

R

N

N

R

R

S

S

P

A

L

S

I

I

R

R

I

I

P

P

A

V

K

V

R

A

G

S

-

P

M

K

S

A

T

R

R

R

V

I

E
|
E

L

V

R
|
R

S

F

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

C

L

F

A

A

C

L

L

L

H

M

A

A

G

G

L

L

D

D

G

G

I

I

K

K

K

N

G

K

M

I

T

H

P

P

P

G

P

E

A

A

T

M

D

D

R

K

N

N

I

L

Y

Y

E

D

M

L

T

P

L

P

A

E

E

E

R

A

Q

K

E

E

L

-

G

-

I

I

S

P

N

Q

L

V

P

A

E

G

N

S

L

L

S

E

E

E

A

A

L

L

K

N

V

A

L

L

A

D

A

L

D

D

N

R

V

E

I

F

K

L

E

K

A

A

L

G

G

G

-

V

-

F

-

T

P

D

H

E

I

A

F

I

E

D

R

A

F

Y

I

I

E

A

A

L

K

R

S

R

I

E

E

E

W

D

D

D

R

R

Y

V

R

R

M

M

Q

T

V

P

S

H

S

P

W

V

E

E

L

F

D

E

E

L

Y

Y

E130 (= E131) binding Mn(2+)
E132 (= E133) binding Mn(2+)
E208 (= E183) binding ATP
E213 (= E188) binding Mn(2+)
E221 (= E195) binding Mn(2+)
NG 265:266 (= NG 239:240) binding L-glutamate
H270 (= H244) binding Mn(2+)
HMS 272:274 (≠ NQS 246:248) binding ATP
R322 (= R298) binding L-glutamate
E358 (= E333) binding Mn(2+)
R360 (= R335) binding L-glutamate

Query Sequence

>WP_013840878.1 NCBI__GCF_000215085.1:WP_013840878.1
MTKLTNDDVRQMCKDLNVKFIRLQFTDIFGVLKNVAITVEQLDKALDGEMMFDGSSIEGF
VRIEESDMYLRPDPSTFVVFPWKPREGGVARLICDIYNPDGTPFFGDPRYALKRALAEAE
QMGYVMNVGPEAEFFLFHVDNEGRPTTKTHDKAGYFDMTPIDLGENARRDMVLTLEQMGF
EIEASHHEVAPGQHEIDFKYADALDMADKIMTFKLVVRTIAQRHGLHATFMPKPVFGING
SGMHVNQSLITLDGKNAFYDPDAPMGLSKVCMNYIAGLMNHADAITAIANPTVNSYKRLV
AGYEAPVYVAWSGRNRSPLIRIPAKRGMSTRVELRSPDPSCNPYLALAACLHAGLDGIKK
GMTPPPATDRNIYEMTLAERQELGISNLPENLSEALKVLAADNVIKEALGPHIFERFIEA
KSIEWDRYRMQVSSWELDEYLEKF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory