SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013841351.1 NCBI__GCF_000215085.1:WP_013841351.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

3ip4B The high resolution structure of gatcab (see paper)
58% identity, 99% coverage: 3:478/479 of query aligns to 2:472/482 of 3ip4B

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3ip4B

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binding magnesium ion: H12 (= H13), E124 (= E125), E150 (= E156)

3al0B Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
53% identity, 100% coverage: 3:479/479 of query aligns to 2:480/482 of 3al0B

query
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3al0B

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binding zinc ion: C25 (≠ S26), C39 (= C39), C42 (= C42)
binding : R225 (= R224), Y319 (= Y318), N349 (= N348), M352 (= M351), T353 (≠ G352), L356 (≠ S355), R357 (= R356), K391 (= K390), K394 (= K393), A439 (≠ S438), D442 (= D441), K449 (= K448), G452 (= G451), F453 (= F452), V455 (= V454), G456 (= G455), P468 (= P467)

2g5iB Structure of tRNA-dependent amidotransferase gatcab complexed with adp-alf4 (see paper)
59% identity, 87% coverage: 3:417/479 of query aligns to 1:410/410 of 2g5iB

query
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2g5iB

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L

L

P

P

V

A

Y

Y

D

D

A

A

G

H

I

V

L

L

T

T

L

L

T

T

K

K

E

E

M

M

S

S

D

D

F

F

F

F

E

E

Q

S

T

T

I

I

Q

E

Q

H

Y

G

D

A

N

D

P

V

K

K

A

L

V

T

S

S

N

N

W

W

M

L

M

M

G

G

E

G

M

V

S

N

R

E

L

Y

L

L

N

N

A

K

G

N

G

Q

M

V

E

E

I

L

V

L

Q

D

C

T

K

K

I

L

K

T

P

P

A

E

Q

N

L

L

A

A

S

G

L

M

L

I

K

K

L

L

I

I

D

E

K

D

G

G

T

T

I

M

S

S

G

S

K

K

I

I

A

A

K

K

T

K

V

V

F

F

E

P

E

E

M

L

F

A

A

A

S

K

G

G

K

G

D

N

P

A

E

K

A

Q

V

I

V

M

Q

E

E

D

K

N

G

G

L

L

V

V

Q

Q

binding adenosine-5'-diphosphate: V5 (≠ I7), E9 (= E11), V151 (= V158), S152 (= S159), P154 (= P161), N193 (= N200), S195 (= S202), K207 (= K214)
binding magnesium ion: H11 (= H13), E123 (= E125), E149 (= E156)

2df4B Structure of tRNA-dependent amidotransferase gatcab complexed with mn2+ (see paper)
60% identity, 84% coverage: 3:403/479 of query aligns to 1:396/399 of 2df4B

query
sites
2df4B

Q

H

Y

F

E

E

T

T

I

V

I

I

G

G

I

L

E
|
E

V

V

H
|
H

V

V

E

E

L

L

K

K

T

T

N

D

T

S

K

K

I

M

F

F

C

S

N

P

S

S

T

P

T

A

E

H

F

F

G

G

G

A

D

E

P

P

N

N

T

S

H

N

V

T

C

N

P

V

V

I

C

D

L

L

G

A

L

Y

P

P

G

G

T

V

L

L

P

P

V

V

L

V

N

N

K

K

K

R

V

A

V

V

E

D

Y

W

A

A

V

M

R

R

A

A

G

A

L

M

A

A

L

L

N

N

C

M

S

E

I

I

A

A

N

T

F

E

S

S

K

K

F

F

D

D

R

R

K

K

N

N

Y

Y

Y

F

Y

Y

P

P

D

D

L

N

T

P

K

K

N

A

Y

Y

Q

Q

I

I

S

S

Q

Q

Y

F

D

D

L

Q

P

P

I

I

A

G

E

E

Q

N

G

G

Y

Y

L

I

D

D

I

I

E

E

V

V

D

D

G

G

Q

E

E

T

K

K

R

R

I

I

G

G

I

I

T

T

R

R

L

L

H

H

M

M

E
|
E

E

E

D

D

A

A

G

G

K

K

L

S

V

T

H

H

Q

K

G

G

N

E

I

-

M

-

T

-

T

-

P

-

Y

Y

S

S

L

L

V

V

D

D

Y

L

N

N

R

R

A

Q

G

G

V

T

P

P

L

L

I

I

E
|
E

I

I

V

V

S

S

E

E

P

P

D

D

M

I

R

R

S

S

P

P

E

K

E

E

A

A

R

Y

A

A

Y

Y

A

L

E

E

K

K

L

L

R

R

A

S

V

I

I

I

Q

Q

Y

Y

T

T

G

G

V

V

S

S

D

D

C

V

R

K

M

M

E

E

E

E

G

G

S

S

L

L

R

R

C

C

D
|
D

A

A

N

N

I

I

S

S

V

L

R

R

P

P

V

Y

G

G

Q

Q

K

E

E

K

L

F

G

G

T

T

K

K

S

A

E
|
E

I

L

K

K

N

N

M

L

N

N

S

S

F

F

R

N

A

Y

L

V

Q

R

R

K

A

G

L

L

A

E

Y

Y

E

E

V

E

E

K

R

R

Q

Q

I

E

G

E

V

E

L

L

E

L

S

N

G

G

G

G

Q

E

V

I

V

G

Q

Q

E

E

T

T

R

R

T

R

W

F

D

D

E

E

A

S

R

T

G

G

I

K

T

T

L

I

S

L

M

M

R

R

S

V

K

K

E

E

Q

G

A

S

H

D

D

D

Y

Y

R

R

Y

Y

F

F

P

P

D

E

P

P

D

D

L

I

V

V

P

P

L

L

V

Y

L

I

D

D

P

D

Q

A

W

W

I

K

D

E

F

R

I

V

R

R

Q

Q

S

T

L

I

P

P

E

E

L

L

P

P

D

D

Q

E

R

R

R

K

A

A

R

K

Y

Y

V

V

Q

N

E

E

F

L

G

G

L

L

P

P

V

A

Y

Y

D

D

A

A

G

H

I

V

L

L

T

T

L

L

T

T

K

K

E

E

M

M

S

S

D

D

F

F

F

F

E

E

Q

S

T

T

I

I

Q

E

Q

H

Y

G

D

A

N

D

P

V

K

K

A

L

V

T

S

S

N

N

W

W

M

L

M

M

G

G

E

G

M

V

S

N

R

E

L

Y

L

L

N

N

A

K

G

N

G

Q

M

V

E

E

I

L

V

L

Q

D

C

T

K

K

I

L

K

T

P

P

A

E

Q

N

L

L

A

A

S

G

L

M

L

I

K

K

L

L

I

I

D

E

K

D

G

G

T

T

I

M

S

S

G

S

K

K

I

I

A

A

K

K

T

K

V

V

F

F

E

P

E

E

M

L

F

A

A

A

S

K

G

G

binding manganese (ii) ion: E9 (= E11), H11 (= H13), E123 (= E125), E149 (= E156), D191 (= D198), E209 (= E216)

3h0rH Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
55% identity, 86% coverage: 3:415/479 of query aligns to 2:410/410 of 3h0rH

query
sites
3h0rH

Q

K

Y

Y

E

E

T

A

I
x
V

I

I

G

G

I

L

E
|
E

V

I

H
|
H

V

V

E

Q

L

M

K

D

T

T

N

K

T

T

K

K

I

M

F

F

C
|
C

N

G

S
x
C

T

K

T

V

E

E

F

F

G

G

G

A

D

E

P

P

N

N

T

T

H

N

V

V

C
|
C

P

P

V

V

C
|
C

L

L

G

G

L

M

P

P

G

G

T

A

L

L

P

P

V

I

L

V

N

N

K

K

K

R

V

A

V

V

E

E

Y

Y

A

A

V

I

R

R

A

A

G

S

L

L

A

A

L

L

N

N

C

C

S

E

I

V

A

H

N

E

F

E

S

S

K

V

F

F

D

A

R

R

K
|
K

N

H

Y

Y

Y

F

Y

Y

P

P

D

D

L

L

T

P

K

K

N

G

Y

Y

Q

Q

I

I

S

S

Q

Q

Y

Y

D

E

L

K

P

P

I

L

A

A

E

T

Q

N

G

G

Y

W

L

V

D

E

I

L

E

N

V

L

-

P

D

N

G

G

Q

E

E

K

K

K

R

K

I

V

G

R

I

I

T

R

R

R

L

L

H

H

M

I

E
|
E

E

E

D

D

A

A

G

G

K

K

L

N

V

I

H

H

Q

E

G

G

N

D

I

-

M

-

T

-

T

-

P

-

Y

K

S

T

L

L

V

V

D

D

Y

L

N

N

R

R

A

A

G

G

V

T

P

P

L

L

I

M

E
|
E

I

I

V
|
V

S
x
T

E

E

P

P

D

D

M

I

R

R

S

T

P

P

E

E

E

E

A

A

R

R

A

L

Y

F

A

L

E

E

K

K

L

L

R

R

A

N

V

I

I

M

Q

R

Y

Y

T

A

G

G

V

V

S

S

D

K

C

A

R

D

M

M

E

E

E

K

G

G

S

Q

L

L

R

R

C

C

D

D

A

I

N
|
N

I

V

S
|
S

V

I

R

R

P

P

V

K

G

G

Q

S

K

K

E

E

L

F

G

G

T

T

K
x
R

S

V

E
|
E

I

I

K

K

N

N

M

V

N

N

S

S

F

F

R

R

A

F

L

V

Q

Q

R

K

A

A

L

L

A

E

Y

Y

E

E

V

I

E

E

R

R

Q

Q

I

I

G

N

V

V

L

V

E

E

S

E

G

G

G

G

Q

E

V

V

V

V

Q

Q

E

E

T

T

R

R

T

T

W

F

D

D

E

P

A

Q

R

T

G

G

I

K

T

T

L

Y

S

P

M

M

R

R

S

T

K

K

E

E

Q

E

A

A

H

E

D

D

Y
|
Y

R

R

Y

Y

F

F

P

P

D

D

P

P

D

D

L

L

V

V

P

P

L

L

V

K

L

V

D

K

P

K

Q

E

W

W

I

I

D

E

F

E

I

I

R

K

Q

K

S

N

L

M

P

P

E

E

L

L

P

P

D

D

Q

Q

R

R

R

F

A

E

R

R

Y

L

V

I

Q

K

E

E

F

Y

G

G

L

L

P

S

V

E

Y

Y

D

E

A

A

G

G

I

I

L

L

T

V

L

N

T

H

K

K

E

E

M

V

S

G

D

D

F

F

F

F

E

E

Q

E

T

A

I

V

Q

R

Q

H

Y

F

D

K

N

E

P

P

K

K

A

G

V

I

S

V

N

N

W

W

M

L

M

I

G

N

E

D

M

L

S

L

R

G

L

L

L

L

N

R

A

D

G

K

G

G

M

I

E

S

I

I

V

E

Q

E

C

S

K

P

I

V

K

K

P

P

A

E

Q

H

L

L

A

A

S

E

L

L

L

V

K

K

L

L

I

I

D

K

K

E

G

K

T

V

I

I

S

S

G

T

K

K

I

I

A

G

K

K

T

E

V

V

F

I

E

K

E

E

M

M

F

V

A

E

S

T

G

G

K

K

D

T

P

P

E

S

A

Q

V

I

V

V

Q

E

E

E

K

K

G

G

L

L

binding aspartic acid: H12 (= H13), K79 (= K80), E151 (= E156), Y268 (= Y273)
binding adenosine-5'-triphosphate: V6 (≠ I7), E10 (= E11), V153 (= V158), T154 (≠ S159), N195 (= N200), S197 (= S202), R209 (≠ K214)
binding manganese (ii) ion: E10 (= E11), H12 (= H13), E125 (= E125), E151 (= E156), E211 (= E216)
binding zinc ion: C23 (= C24), C25 (≠ S26), C38 (= C39), C41 (= C42)

3h0rE Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
55% identity, 86% coverage: 3:415/479 of query aligns to 2:410/410 of 3h0rE

query
sites
3h0rE

Q

K

Y

Y

E

E

T

A

I
x
V

I

I

G

G

I

L

E
|
E

V

I

H
|
H

V

V

E

Q

L

M

K

D

T

T

N

K

T

T

K

K

I

M

F

F

C
|
C

N

G

S
x
C

T

K

T

V

E

E

F

F

G

G

G

A

D

E

P

P

N

N

T

T

H

N

V

V

C
|
C

P

P

V

V

C
|
C

L

L

G

G

L

M

P

P

G

G

T

A

L

L

P

P

V

I

L

V

N

N

K

K

K

R

V

A

V

V

E

E

Y

Y

A

A

V

I

R

R

A

A

G

S

L

L

A

A

L

L

N

N

C

C

S

E

I

V

A

H

N

E

F

E

S

S

K

V

F

F

D

A

R

R

K

K

N

H

Y

Y

Y

F

Y

Y

P

P

D

D

L

L

T

P

K

K

N

G

Y

Y

Q

Q

I

I

S

S

Q

Q

Y

Y

D

E

L

K

P

P

I

L

A

A

E

T

Q

N

G

G

Y

W

L

V

D

E

I

L

E

N

V

L

-

P

D

N

G

G

Q

E

E

K

K

K

R

K

I

V

G

R

I

I

T

R

R

R

L

L

H

H

M

I

E
|
E

E

E

D

D

A

A

G

G

K

K

L

N

V

I

H

H

Q

E

G

G

N

D

I

-

M

-

T

-

T

-

P

-

Y

K

S

T

L

L

V

V

D

D

Y

L

N

N

R

R

A

A

G

G

V

T

P

P

L

L

I

M

E
|
E

I

I

V

V

S
x
T

E

E

P
|
P

D

D

M

I

R

R

S

T

P

P

E

E

E

E

A

A

R

R

A

L

Y

F

A

L

E

E

K

K

L

L

R

R

A

N

V

I

I

M

Q

R

Y

Y

T

A

G

G

V

V

S

S

D

K

C

A

R

D

M

M

E

E

E

K

G

G

S

Q

L

L

R

R

C

C

D

D

A

I

N
|
N

I

V

S
|
S

V

I

R

R

P

P

V

K

G

G

Q

S

K

K

E

E

L

F

G

G

T

T

K
x
R

S

V

E
|
E

I

I

K

K

N

N

M

V

N

N

S

S

F

F

R

R

A

F

L

V

Q

Q

R

K

A

A

L

L

A

E

Y

Y

E

E

V

I

E

E

R

R

Q

Q

I

I

G

N

V

V

L

V

E

E

S

E

G

G

G

G

Q

E

V

V

V

V

Q

Q

E

E

T

T

R

R

T

T

W

F

D

D

E

P

A

Q

R

T

G

G

I

K

T

T

L

Y

S

P

M

M

R

R

S

T

K

K

E

E

Q

E

A

A

H

E

D

D

Y

Y

R

R

Y

Y

F

F

P

P

D

D

P

P

D

D

L

L

V

V

P

P

L

L

V

K

L

V

D

K

P

K

Q

E

W

W

I

I

D

E

F

E

I

I

R

K

Q

K

S

N

L

M

P

P

E

E

L

L

P

P

D

D

Q

Q

R

R

R

F

A

E

R

R

Y

L

V

I

Q

K

E

E

F

Y

G

G

L

L

P

S

V

E

Y

Y

D

E

A

A

G

G

I

I

L

L

T

V

L

N

T

H

K

K

E

E

M

V

S

G

D

D

F

F

F

F

E

E

Q

E

T

A

I

V

Q

R

Q

H

Y

F

D

K

N

E

P

P

K

K

A

G

V

I

S

V

N

N

W

W

M

L

M

I

G

N

E

D

M

L

S

L

R

G

L

L

L

L

N

R

A

D

G

K

G

G

M

I

E

S

I

I

V

E

Q

E

C

S

K

P

I

V

K

K

P

P

A

E

Q

H

L

L

A

A

S

E

L

L

L

V

K

K

L

L

I

I

D

K

K

E

G

K

T

V

I

I

S

S

G

T

K

K

I

I

A

G

K

K

T

E

V

V

F

I

E

K

E

E

M

M

F

V

A

E

S

T

G

G

K

K

D

T

P

P

E

S

A

Q

V

I

V

V

Q

E

E

E

K

K

G

G

L

L

binding adenosine-5'-triphosphate: V6 (≠ I7), E10 (= E11), T154 (≠ S159), P156 (= P161), N195 (= N200), S197 (= S202), R209 (≠ K214)
binding manganese (ii) ion: E10 (= E11), H12 (= H13), E125 (= E125), E151 (= E156), E211 (= E216)
binding zinc ion: C23 (= C24), C25 (≠ S26), C38 (= C39), C41 (= C42)

3h0rB Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
55% identity, 86% coverage: 3:415/479 of query aligns to 2:410/410 of 3h0rB

query
sites
3h0rB

Q

K

Y

Y

E

E

T

A

I

V

I

I

G
|
G

I
x
L

E
|
E

V

I

H
|
H

V

V

E

Q

L

M

K

D

T

T

N

K

T

T

K

K

I

M

F

F

C
|
C

N

G

S
x
C

T

K

T

V

E

E

F

F

G

G

G

A

D

E

P

P

N

N

T

T

H

N

V

V

C
|
C

P

P

V

V

C
|
C

L

L

G

G

L

M

P

P

G

G

T

A

L

L

P

P

V

I

L

V

N

N

K

K

K

R

V

A

V

V

E

E

Y

Y

A

A

V

I

R

R

A

A

G

S

L

L

A

A

L

L

N

N

C

C

S

E

I

V

A

H

N

E

F

E

S

S

K

V

F

F

D

A

R

R

K

K

N

H

Y

Y

Y

F

Y

Y

P

P

D

D

L

L

T

P

K

K

N

G

Y

Y

Q

Q

I

I

S

S

Q

Q

Y

Y

D

E

L

K

P

P

I

L

A

A

E

T

Q

N

G

G

Y

W

L

V

D

E

I

L

E

N

V

L

-

P

D

N

G

G

Q

E

E

K

K

K

R

K

I

V

G

R

I

I

T

R

R

R

L

L

H

H

M

I

E
|
E

E

E

D

D

A

A

G

G

K

K

L

N

V

I

H

H

Q

E

G

G

N

D

I

-

M

-

T

-

T

-

P

-

Y

K

S

T

L

L

V

V

D

D

Y

L

N

N

R

R

A

A

G

G

V

T

P

P

L

L

I

M

E
|
E

I

I

V

V

S
x
T

E

E

P
|
P

D

D

M

I

R

R

S

T

P

P

E

E

E

E

A

A

R

R

A

L

Y

F

A

L

E

E

K

K

L

L

R

R

A

N

V

I

I

M

Q

R

Y

Y

T

A

G

G

V

V

S

S

D

K

C

A

R

D

M

M

E

E

E

K

G

G

S

Q

L

L

R

R

C

C

D

D

A

I

N
|
N

I

V

S
|
S

V

I

R

R

P

P

V

K

G

G

Q

S

K

K

E

E

L

F

G

G

T

T

K
x
R

S

V

E
|
E

I

I

K

K

N

N

M

V

N

N

S

S

F

F

R

R

A

F

L

V

Q

Q

R

K

A

A

L

L

A

E

Y

Y

E

E

V

I

E

E

R

R

Q

Q

I

I

G

N

V

V

L

V

E

E

S

E

G

G

G

G

Q

E

V

V

V

V

Q

Q

E

E

T

T

R

R

T

T

W

F

D

D

E

P

A

Q

R

T

G

G

I

K

T

T

L

Y

S

P

M

M

R

R

S

T

K

K

E

E

Q

E

A

A

H

E

D

D

Y

Y

R

R

Y

Y

F

F

P

P

D

D

P

P

D

D

L

L

V

V

P

P

L

L

V

K

L

V

D

K

P

K

Q

E

W

W

I

I

D

E

F

E

I

I

R

K

Q

K

S

N

L

M

P

P

E

E

L

L

P

P

D

D

Q

Q

R

R

R

F

A

E

R

R

Y

L

V

I

Q

K

E

E

F

Y

G

G

L

L

P

S

V

E

Y

Y

D

E

A

A

G

G

I

I

L

L

T

V

L

N

T

H

K

K

E

E

M

V

S

G

D

D

F

F

F

F

E

E

Q

E

T

A

I

V

Q

R

Q

H

Y

F

D

K

N

E

P

P

K

K

A

G

V

I

S

V

N

N

W

W

M

L

M

I

G

N

E

D

M

L

S

L

R

G

L

L

L

L

N

R

A

D

G

K

G

G

M

I

E

S

I

I

V

E

Q

E

C

S

K

P

I

V

K

K

P

P

A

E

Q

H

L

L

A

A

S

E

L

L

L

V

K

K

L

L

I

I

D

K

K

E

G

K

T

V

I

I

S

S

G

T

K

K

I

I

A

G

K

K

T

E

V

V

F

I

E

K

E

E

M

M

F

V

A

E

S

T

G

G

K

K

D

T

P

P

E

S

A

Q

V

I

V

V

Q

E

E

E

K

K

G

G

L

L

binding adenosine-5'-diphosphate: G8 (= G9), L9 (≠ I10), E10 (= E11), T154 (≠ S159), P156 (= P161), N195 (= N200), S197 (= S202), R209 (≠ K214)
binding manganese (ii) ion: E10 (= E11), H12 (= H13), E125 (= E125), E151 (= E156), N195 (= N200), E211 (= E216)
binding zinc ion: C23 (= C24), C25 (≠ S26), C38 (= C39), C41 (= C42)

3h0lB Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
55% identity, 86% coverage: 3:415/479 of query aligns to 2:410/410 of 3h0lB

query
sites
3h0lB

Q

K

Y

Y

E

E

T

A

I
x
V

I

I

G
|
G

I
x
L

E
|
E

V

I

H
|
H

V

V

E

Q

L

M

K

D

T

T

N

K

T

T

K

K

I

M

F

F

C
|
C

N

G

S
x
C

T

K

T

V

E

E

F

F

G

G

G

A

D

E

P

P

N

N

T

T

H

N

V

V

C
|
C

P

P

V

V

C
|
C

L

L

G

G

L

M

P

P

G

G

T

A

L

L

P

P

V

I

L

V

N

N

K

K

K

R

V

A

V

V

E

E

Y

Y

A

A

V

I

R

R

A

A

G

S

L

L

A

A

L

L

N

N

C

C

S

E

I

V

A

H

N

E

F

E

S

S

K

V

F

F

D

A

R

R

K

K

N

H

Y

Y

Y

F

Y

Y

P

P

D

D

L

L

T

P

K

K

N

G

Y

Y

Q

Q

I

I

S

S

Q

Q

Y

Y

D

E

L

K

P

P

I

L

A

A

E

T

Q

N

G

G

Y

W

L

V

D

E

I

L

E

N

V

L

-

P

D

N

G

G

Q

E

E

K

K

K

R

K

I

V

G

R

I

I

T

R

R

R

L

L

H

H

M

I

E
|
E

E

E

D

D

A

A

G

G

K

K

L

N

V

I

H

H

Q

E

G

G

N

D

I

-

M

-

T

-

T

-

P

-

Y

K

S

T

L

L

V

V

D

D

Y

L

N

N

R

R

A

A

G

G

V

T

P

P

L

L

I

M

E
|
E

I

I

V
|
V

S
x
T

E

E

P
|
P

D

D

M

I

R

R

S

T

P

P

E

E

E

E

A

A

R

R

A

L

Y

F

A

L

E

E

K

K

L

L

R

R

A

N

V

I

I

M

Q

R

Y

Y

T

A

G

G

V

V

S

S

D

K

C

A

R

D

M

M

E

E

E

K

G

G

S

Q

L

L

R

R

C

C

D

D

A

I

N
|
N

I

V

S
|
S

V

I

R

R

P

P

V

K

G

G

Q

S

K

K

E

E

L

F

G

G

T

T

K
x
R

S

V

E

E

I

I

K

K

N

N

M

V

N

N

S

S

F

F

R

R

A

F

L

V

Q

Q

R

K

A

A

L

L

A

E

Y

Y

E

E

V

I

E

E

R

R

Q

Q

I

I

G

N

V

V

L

V

E

E

S

E

G

G

G

G

Q

E

V

V

V

V

Q

Q

E

E

T

T

R

R

T

T

W

F

D

D

E

P

A

Q

R

T

G

G

I

K

T

T

L

Y

S

P

M

M

R

R

S

T

K

K

E

E

Q

E

A

A

H

E

D

D

Y

Y

R

R

Y

Y

F

F

P

P

D

D

P

P

D

D

L

L

V

V

P

P

L

L

V

K

L

V

D

K

P

K

Q

E

W

W

I

I

D

E

F

E

I

I

R

K

Q

K

S

N

L

M

P

P

E

E

L

L

P

P

D

D

Q

Q

R

R

R

F

A

E

R

R

Y

L

V

I

Q

K

E

E

F

Y

G

G

L

L

P

S

V

E

Y

Y

D

E

A

A

G

G

I

I

L

L

T

V

L

N

T

H

K

K

E

E

M

V

S

G

D

D

F

F

F

F

E

E

Q

E

T

A

I

V

Q

R

Q

H

Y

F

D

K

N

E

P

P

K

K

A

G

V

I

S

V

N

N

W

W

M

L

M

I

G

N

E

D

M

L

S

L

R

G

L

L

L

L

N

R

A

D

G

K

G

G

M

I

E

S

I

I

V

E

Q

E

C

S

K

P

I

V

K

K

P

P

A

E

Q

H

L

L

A

A

S

E

L

L

L

V

K

K

L

L

I

I

D

K

K

E

G

K

T

V

I

I

S

S

G

T

K

K

I

I

A

G

K

K

T

E

V

V

F

I

E

K

E

E

M

M

F

V

A

E

S

T

G

G

K

K

D

T

P

P

E

S

A

Q

V

I

V

V

Q

E

E

E

K

K

G

G

L

L

binding adenosine-5'-diphosphate: V6 (≠ I7), G8 (= G9), L9 (≠ I10), E10 (= E11), V153 (= V158), T154 (≠ S159), P156 (= P161), N195 (= N200), S197 (= S202), R209 (≠ K214)
binding magnesium ion: H12 (= H13), E125 (= E125), E151 (= E156)
binding zinc ion: C23 (= C24), C25 (≠ S26), C38 (= C39), C41 (= C42)

4wj3B Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
51% identity, 83% coverage: 4:399/479 of query aligns to 1:392/456 of 4wj3B

query
sites
4wj3B

Y

W

E

E

T

T

I

V

I

I

G

G

I

L

E

E

V

I

H

H

V

A

E

Q

L

L

K

A

T

T

N

Q

T

S

K

K

I

I

F

F

C

S

N

G

S

S

T

S

T

T

E

A

F

F

G

G

G

A

D

A

P

P

N

N

T

T

H

Q

V

A

C

S

P

L

V

V

C

D

L

L

G

A

L

M

P

P

G

G

T

T

L

L

P

P

V

V

L

L

N

N

K

E

K

E

V

A

V

V

E

R

Y

M

A

A

V

C

R

L

A

F

G

G

L

L

A

A

L

I

N

D

C

A

S

R

I

I

A

D

N

R

F

Q

S

N

K

V

F

F

D

A

R

R

K

K

N

N

Y

Y

Y

F

Y

Y

P

P

D

D

L

L

T

P

K

K

N

G

Y

Y

Q

Q

I

T

S

S

Q

Q

Y

M

D

D

L

H

P

P

I

I

A

V

E

G

Q

K

G

G

Y

H

L

L

D

D

I

I

E

T

V

L

-

E

D

D

G

G

Q

T

E

T

K

K

R

R

I

I

G

G

I

I

T

T

R

R

L

A

H

H

M

L

E

E

E

E

D

D

A

A

G

G

K
|
K

L

S

V

L

H

H

Q

E

G

D

N

G

I

M

M

-

T

-

T

-

P

-

Y

-

S

S

L

G

V

I

D

D

Y

L

N

N

R

R

A

A

G

G

V

T

P

P

L

L

I

L

E

E

I

I

V

V

S

S

E

E

P

P

D

D

M

I

R

R

S

S

P

A

E

K

E

E

A

A

R

V

A

A

Y

Y

A

V

E

K

K

A

L

I

R

H

A

A

V

L

I

V

Q

R

Y

Y

T

L

G

G

V

I

S

C

D

D

C

G

R

N

M

M

E
x
A

E

E

G

G

S

S

L

L

R

R

C

C

D

D

A

C

N

N

I

V

S

S

V

V

R

R

P

P

V

K

G

G

Q

Q

K

A

E

E

L
x
F

G

G

T

T

K

R

S

A

E

E

I

I

K

K

N
|
N

M

V

N

N

S
|
S

F

F

R
|
R

A

F

L

I

Q

E

R

K

A

A

L

I

A

N

Y

H

E

E

V

I

E

Q

R

R

Q

Q

I

I

G

E

V

L

L

I

E

E

S

D

G

G

G

G

Q

K

V
|
V

V

V

Q

Q

E

E

T

T

R

R

T

L

W

Y

D

D

E

P

A

N

R

K

G

D

I

E

T

T

L

R

S

S

M

M

R

R

S

G

K

K

E

E

Q

E

A

A

H

N

D

D

Y

Y

R

R

Y

Y

F

F

P

P

D

C

P

P

D

D

L

L

V

L

P

P

L

V

V

V

L

I

D

E

P

P

Q

E

W

Y

I

L

D

A

F

K

I

L

R

R

Q

E

S

Q

L

L

P

P

E

E

L

L

P

P

D

V

Q

Q

R

K

R

R

A

E

R

R

Y

F

V

E

Q

S

E

Q

F

Y

G

G

L

L

P

S

V

A

Y
|
Y

D

D

A

A

G

S

I

V

L

L

T

S

L

A

T

S

K

R

E

E

M

M

S

A

D

D

F

Y

F

F

E

E

Q

K

T

V

I

Q

Q

G

Q

I

Y

C

D

G

N

D

P

A

K

K

A

L

V

A

S

A

N
|
N

W

W

M

V

M
|
M

G
x
V

E

E

M

L

S

G

R
x
S

L

L

L

L

N
|
N

A

K

G

D

G

G

M

L

E

E

I

I

V

E

Q

Q

C

S

K

P

I

V

K

S

P

A

A

E

Q

Q

L

L

A

G

S

G

L

M

L

I

K

L

L

R

I

I

D

K

K

D

G

N

T

T

I

I

S

S

G

G

K

K

I

L

A

A

K

K

T

M

V

V

F

F

E

E

E

A

M

M

binding : K128 (= K130), A184 (≠ E191), F204 (≠ L211), N212 (= N219), S215 (= S222), R217 (= R224), V240 (= V247), Y311 (= Y318), N341 (= N348), M344 (= M351), V345 (≠ G352), S349 (≠ R356), N352 (= N359)

Sites not aligning to the query:

binding : 430, 431, 434

3kfuF Crystal structure of the transamidosome (see paper)
46% identity, 84% coverage: 4:407/479 of query aligns to 2:389/447 of 3kfuF

query
sites
3kfuF

Y

Y

E

E

T

A

I

V

I

I

G

G

I

L

E

E

V

V

H
|
H

V

L

E

H

L

L

K

K

T

T

N

R

T

T

K

K

I

M

F

F

C
|
C

N

G

S
x
C

T

R

T

A

E

D

-

Y

F

F

G

G

G

A

D

E

P

P

N

N

T

T

H

H

V

T

C
|
C

P

P

V

V

C

C

L

L

G

G

L

L

P

P

G

G

T

A

L

L

P

P

V

V

L

P

N

N

K

R

K

V

V

A

V

V

E

E

Y

H

A

G

V

L

R

R

A

L

G

A

L

L

A

A

L

L

N

G

C

A

S

E

I

V

A

P

N

E

F

R

S

L

K

V

F

F

D

H

R

R

K

K

N

N

Y
|
Y

Y

F

Y
|
Y

P

P

D
|
D

L

L

T

P

K

K

N

N

Y

Y

Q

Q

I

I

S

S

Q

Q

Y
|
Y

D
|
D

L

L

P

P

I

L

A

G

E

R

Q

G

G

G

Y

S

L

L

D

P

I

L

E

-

V

-

D

-

G

-

Q

G

E

E

K

R

R

R

I

V

G

R

I

I

T

K

R

R

L

L

H

H

M

L

E
|
E

E

E

D

D

A

A

G

G

K
|
K

L

S

V

L

H
|
H

-

L

Q

E

G

G

N

R

I

-

M

-

T

-

T

-

P

-

Y

-

S

T

L

L

V

L

D

D

Y

L

N

N

R
|
R

A

A

G

G

V

S

P

P

L

L

I

I

E
|
E

I

L

V

V

S

T

E

E

P

P

D

D

M

L

R

K

S

T

P

P

E

E

E

E

A

A

R
|
R

A

L

Y

F

A

L

E

Q

K

R

L

I

R

Q

A

A

V

L

I

V

Q

Q

Y

T

T

L

G

G

V

I

S

S

D

D

C

A

R

S

M
x
P

E
|
E

E

E

G
|
G

S

K

L

L

R
|
R

C

A

D

D

A

V

N

N

I

V

S

S

V

V

R

R

P

R

V

V

G

G

Q

E

K

P

E

-

L

L

G

G

T

T

K

K

S

V

E

E

I

I

K

K

N

N

M

L

N
|
N

S
|
S

F
|
F

R
x
K

A

S

L

V

Q

Q

R

R

A

A

L

L

A

E

Y

Y

E

E

V

I

E

R

R

R

Q

Q

I

T

G

E

V

I

L

L

E

R

S

R

G

G

G

E

Q

K

V

V

V

K

Q

Q

E

A

T

T

R

M

T

G

W

F

D

E

E

E

A

G

R

S

G

G

I

K

T

T

L

Y

S

P

M

M

R

-

S

-

K

-

E

-

Q

-

A

-

H

A

D

D

Y

Y

R

R

Y

Y

F

F

P

P

D

E

P

P

D

D

L

L

V

P

P

P

L

V

V

A

L

I

D

P

P

R

Q

D

W

W

I

L

D

E

F

E

I

V

R

R

Q

R

S

S

L

L

P

P

E

E

L

L

P

P

D

W

Q

E

R

K

R

E

A

A

R

R

Y

Y

V

-

Q

R

E

A

F

L

G

G

L

I

P

K

V

E

Y

K

D

D

A

A

G

E

I

V

L

L

T

A

L

Y

T

T

K

P

E

S

M

L

S

A

D

R

F

F

F

L

E

D

Q

Q

T

A

I

L

Q

P

-

L

Q

G

Y

L

D

A

N

S

P

P

K

Q

A

A

V

L

S

A

N

N

W

W

M

L

M
x
L

G

A

E

D

M

V

S

A

R
x
G

L

L

L

L

N
x
H

A

E

G

R

G

G

M

L

E

R

I

L

V

E

Q

E

C

T

K

R

I

L

K

S

P

P

A

E

Q

G

L

L

A

A

S

R

L

L

L

V

K

G

L

L

I

F

D

E

K

R

G

G

T

E

I

V

S

T

G
x
S

K
x
R

I

V

A

A

K
|
K

T

S

V

L

F

L

E

P

E

E

M

V

F

L

A

-

S

E

G

G

K

Q

D

D

P

P

E

E

binding magnesium ion: H11 (= H13), E120 (= E125), E146 (= E156)
binding zinc ion: C22 (= C24), C24 (≠ S26), C38 (= C39)
binding : Y81 (= Y82), Y83 (= Y84), D85 (= D86), Y95 (= Y96), D96 (= D97), K125 (= K130), H128 (= H133), R139 (= R149), R160 (= R170), P180 (≠ M190), E181 (= E191), G183 (= G193), R186 (= R196), N210 (= N221), S211 (= S222), F212 (= F223), K213 (≠ R224), L334 (≠ M351), G339 (≠ R356), H342 (≠ N359), S372 (≠ G389), R373 (≠ K390), K376 (= K393)

Sites not aligning to the query:

binding : 410, 413, 421, 422, 425, 435

2d6fD Crystal structure of glu-tRNA(gln) amidotransferase in the complex with tRNA(gln) (see paper)
24% identity, 49% coverage: 8:242/479 of query aligns to 10:253/522 of 2d6fD

query
sites
2d6fD

I

M

G

G

I

L

E

E

V

I

H

H

V

Q

E

Q

L

L

K

D

T

T

N

E

T

S

K

K

I

L

F

F

C

C

N

P

S
x
C

T

R

T

T

E

E

F

L

-

T

G

D

G

S

D

E

P

P

N

D

T

H

H

D

V

I

C

V

-

R

-

N

-

L

-

R

-

P

-

T

-

R

-

A

-

A

-

F

-

E

-

E

-

A

-

M

-

R

-

K

-

L

-

H

-

F

-

H

-

Y

-

E

-

N

-

Y

-

H

-

E

P

E

V

T

C
|
C

L

L

G

V

L
x
E

P

A

G

D

T

E

L

E

P

P

V

P

-

H

-

P

L

L

N

N

K

P

K

E

V

A

V

L

E

E

Y

I

A

A

V

V

R

T

A

I

G

A

L

L

A

L

L

L

N

N

C

M

S

R

I

V

A

V

N

D

F

E

S

F

K

H

F

T

D

M

R

R

K

K

N

Q

Y

V

Y

I

Y

D

P

G

D

S

L

N

T

T

K

G

N

G

Y

F

Q

Q

I

R

S

T

Q

G

Y

L

D

-

L

-

P

-

I

V

A

A

E

T

Q

D

G

G

Y

H

L

L

D

E

I

T

E

-

V

-

D

-

G

-

Q

P

E

Q

K

G

R

T

I

V

G

K

I

I

T

E

R

N

L

L

H

C

M

L

E

E

E

E

D

D

A

A

G

A

K

R

L

R

V

I

H

R

Q

E

G

-

N

-

I

-

M

-

T

T

T

G

P

D

Y

G

S

V

L

V

V

F

D

R

Y

L

N

D

R

R

A

L

G

G

V

I

P

P

L

L

I

V

E

E

I

I

V

T

S

T

E

D

P

P

D

S

M

M

R

S

S

D

P

P

E

Q

E

Q

A

L

R

R

A

E

Y

V

A

A

E

Y

K

Q

L

I

R

G

A

Q

V

I

I

L

Q

R

Y

S

T

T

G

R

V

V

S

-

D

-

C

-

R

K

M

R

E

G

E

L

G

G

S

T

L

I

R

R

C

Q

D

D

A

L

N

N

I

I

S

S

V

I

R

R

P

D

V

-

G

-

Q

-

K

-

E

-

L

-

G

G

T

A

K

R

S

V

E

E

I

V

K

K

N

G

M

V

N
x
Q

S
x
D

F

L

R
x
D

A

L

L

I

Q

P

R

E

A

I

L

V

A

E

Y

R

E

E

V

V

E

K

R

R

Q

Q

I

L

G

S

V

L

L

V

E

E

binding zinc ion: C28 (