SitesBLAST

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 69% coverage: 40:191/220 of query aligns to 692:840/1227 of P13009

query
sites
P13009

V

V

V

I

E
|
E

E

G

G

P

L

L

T

M

K

D

A

G

L

M

E

N

S

V

L

V

S

G

D

D

K

L

C

F

G

G

N

E

D

G

Q

K

F

M

S

F

L

L

L

P

E

Q

I

V

L

V

L

K

A

S

G

A

R

R

A

-

M

-

D

-

V

V

M

M

E

K

Q

Q

V

A

V

V

S

A

K

Y

H

L

V

E

C

P

F

F

-

I

-

E

S

A

T

S

L

K

A

E

M

Q

G

G

K

K

E

T

Q

N

P

G

V

K

F

M

V

V

I

I

G

A

T

T

I

V

K

K

G
|
G

D
|
D

M
x
V

H
|
H

D
|
D

L

I

G

G

K

K

N

N

I

I

V

V

S

G

M

V

L

L

K

Q

V

C

A

N

G

N

Y

Y

R

E

V

I

I

V

D

D

L

L

G

G

K

V

D

M

V

V

E

P

P

A

A

E

K

K

F

I

I

L

E

R

T

T

A

A

A

K

A

E

E

V

Q

N

A

A

R

D

Y

L

I

I

G

G

I

L

S
|
S

S

G

L

L

I

I

T
|
T

T

P

T
x
S

I

L

P

D

Y

E

V

M

K

V

E

N

V

V

K

A

H

K

L

E

A

M

V

E

Q

R

E

Q

G

G

M

F

S

T

D

-

V

I

K

P

V

L

L

L

A

I

G

G

A

A

A

T

L

T

R

S

Q

K

A

A

E694 (= E42) binding
GDVHD 756:760 (≠ GDMHD 106:110) binding
D757 (= D107) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H109) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S154) binding
T808 (= T158) binding
S810 (≠ T160) mutation to A: Decreases activity by about 40%.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
247 binding
310 binding ; mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
311 binding ; mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
860 binding
946 binding
1134 binding
1189:1190 binding

1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
32% identity, 69% coverage: 40:191/220 of query aligns to 42:190/246 of 1bmtA

query
sites
1bmtA

V

V

V

I

E
|
E

E

G

G

P

L

L

T
x
M

K

D

A

G

L
x
M

E

N

S

V

L

V

S
x
G

D

D

K

L

C

F

G

G

N

E

D

G

Q

K

F

M

S

F

L
|
L

L

P

E

Q

I
x
V

L

V

L

K

A

S

G

A

R

R

A

-

M

-

D

-

V

V

M

M

E

K

Q

Q

V

A

V

V

S

A

K

Y

H

L

V

E

C

P

F

F

-

I

-

E

S

A

T

S

L

K

A

E

M

Q

G

G

K

K

E

T

Q

N

P

G

V

K

F

M

V

V

I

I

G

A

T

T

I

V

K

K

G

G

D
|
D

M
x
V

H
|
H

D
|
D

L
x
I

G

G

K

K

N

N

I
|
I

V

V

S

G

M

V

L

L

K

Q

V

C

A

N

G

N

Y

Y

R

E

V

I

I

V

D

D

L

L

G

G

K

V

D

M

V

V

E

P

P

A

A

E

K

K

F

I

I

L

E

R

T

T

A

A

A

K

A

E

E

V

Q

N

A

A

R

D

Y

L

I

I

G
|
G

I
x
L

S
|
S

S

G

L
|
L

I
|
I

T
|
T

T

P

T
x
S

I

L

P

D

Y

E

V

M

K

V

E

N

V

V

K

A

H

K

L

E

A

M

V

E

Q

R

E

Q

G

G

M

F

S

T

D

-

V

I

K

P

V

L

L

L

A

I

G
|
G

A
|
A

A

T

L

T

R

S

Q

K

A

A

active site: D107 (= D107), H109 (= H109), S160 (≠ T160)
binding co-methylcobalamin: E44 (= E42), M48 (≠ T46), M51 (≠ L49), G55 (≠ S53), L65 (= L63), V68 (≠ I66), D107 (= D107), V108 (≠ M108), H109 (= H109), D110 (= D110), I111 (≠ L111), I115 (= I115), G152 (= G152), L153 (≠ I153), S154 (= S154), L156 (= L156), I157 (= I157), T158 (= T158), G183 (= G184), G184 (= G185), A185 (= A186)

Sites not aligning to the query:

binding co-methylcobalamin: 207, 209, 210

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
32% identity, 69% coverage: 40:191/220 of query aligns to 42:190/576 of 3ivaA

query
sites
3ivaA

V

V

V

I

E

E

E

G

G

P

L

L

T

M

K

D

A

G

L

M

E

N

S

V

L

V

S

G

D

D

K

L

C

F

G

G

N

E

D

G

Q

K

F

M

S

F

L

L

L

P

E

Q

I

V

L

V

L

K

A

S

G

A

R

R

A

-

M

-

D

-

V

V

M

M

E

K

Q

Q

V

A

V

V

S

A

K

Y

H

L

V

E

C

P

F

F

-

I

-

E

S

A

T

S

L

K

A

E

M

Q

G

C

K

K

E

T

Q

N

P

G

V

K

F

M

V

V

I

I

G

A

T

T

I

V

K

K

G

G

D
|
D

M

V

H
|
H

D

D

L

I

G
|
G

K

K

N

N

I

I

V
|
V

S

G

M

V

L

L

K

Q

V

C

A

N

G

N

Y

Y

R

E

V

I

I

V

D

D

L

L

G

G

K

V

D

M

V

V

E

P

P

A

A

E

K

K

F

I

I

L

E

R

T

T

A

A

A

K

A

E

E

V

Q

N

A

A

R

D

Y

L

I

I

G
|
G

I
x
L

S
|
S

S

G

L
|
L

I
|
I

T
|
T

T

P

T
x
S

I

L

P

D

Y

E

V

M

K

V

E

N

V

V

K

A

H

K

L

E

A

M

V

E

Q

R

E

Q

G

G

M

F

S

T

D

-

V

I

K

P

V

L

L

L

A

I

G
|
G

A

A

A

T

L

T

R

S

Q

K

A

A

active site: D107 (= D107), H109 (= H109), S160 (≠ T160)
binding cobalamin: H109 (= H109), G112 (= G112), V116 (= V116), G152 (= G152), L153 (≠ I153), S154 (= S154), L156 (= L156), I157 (= I157), T158 (= T158), G183 (= G184), G184 (= G185)

Sites not aligning to the query:

binding cobalamin: 208, 209, 303, 443, 486, 488, 489, 495, 520, 521, 524, 527, 528
binding s-adenosyl-l-homocysteine: 447, 484, 485, 489, 491, 539

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
32% identity, 69% coverage: 40:191/220 of query aligns to 42:190/577 of 3bulA

query
sites
3bulA

V

V

V

I

E

E

E

G

G

P

L

L

T

M

K

D

A

G

L

M

E

N

S

V

L

V

S

G

D

D

K

L

C

F

G

G

N

E

D

G

Q

K

F

M

S

F

L

L

L

P

E

Q

I

V

L

V

L

K

A

S

G

A

R

R

A

-

M

-

D

-

V

V

M

M

E

K

Q

Q

V

A

V

V

S

A

K

Y

H

L

V

E

C

P

F

F

-

I

-

E

S

A

T

S

L

K

A

E

M

Q

G

C

K

K

E

T

Q

N

P

G

V

K

F

M

V

V

I

I

G

A

T

T

I

V

K

K

G

G

D
|
D

M

V

H
|
H

D

D

L

I

G

G

K

K

N

N

I

I

V
|
V

S

G

M

V

L

L

K

Q

V

C

A

N

G

N

Y

Y

R

E

V

I

I

V

D

D

L

L

G

G

K

V

D

M

V

V

E

P

P

A

A

E

K

K

F

I

I

L

E

R

T

T

A

A

A

K

A

E

E

V

Q

N

A

A

R

D

Y

L

I

I

G
|
G

I
x
L

S
|
S

S

G

L
|
L

I
|
I

T
|
T

T

P

T
x
S

I

L

P

D

Y

E

V

M

K

V

E

N

V

V

K

A

H

K

L

E

A

M

V

E

Q

R

E

Q

G

G

M

F

S

T

D

-

V

I

K

P

V

L

L

L

A

I

G
|
G

A

A

A

T

L

T

R

S

Q

K

A

A

active site: D107 (= D107), H109 (= H109), S160 (≠ T160)
binding cobalamin: H109 (= H109), V116 (= V116), G152 (= G152), L153 (≠ I153), S154 (= S154), L156 (= L156), I157 (= I157), T158 (= T158), G183 (= G184), G184 (= G185)

Sites not aligning to the query:

binding cobalamin: 208, 209, 210, 213, 302, 443, 486, 487, 488, 489, 495, 498, 521, 524, 527, 528

2i2xB Crystal structure of methanol:cobalamin methyltransferase complex mtabc from methanosarcina barkeri (see paper)
30% identity, 72% coverage: 63:220/220 of query aligns to 95:236/258 of 2i2xB

query
sites
2i2xB

L

L

L

P

E

N

I

V

L

M

A

S

G

A

R

D

A

A

M

M

M

L

D

E

V

G

M

I

E

E

Q

-

V

-

S

-

K

-

H

Y

V

C

C

K

F

E

S

N

T

S

L

G

A

A

M

T

G

P

K

K

E

T

Q

K

P

G

V

T

F

V

V

V

I

C

G

H

T

V

I

A

K

E

G
|
G

D
|
D

M
x
V

H
|
H

D
|
D

L
x
I

G
|
G

K

K

N

N

I

I

V
|
V

S

T

M

A

M

L

L

L

K

R

V

A

A

N

G

G

Y

Y

R

N

V

V

I

V

D

D

L

L

G

G

K

R

D

D

V

V

E

P

P

A

A

E

K

E

F

V

I

L

E

A

T

A

A

V

A

Q

A

K

E

E

Q

K

A

P

R

I

Y

M

I

L

G
x
T

I

G

S
x
T

S

A

L
|
L

I
x
M

T
|
T

T

T

T
|
T

I

M

P

Y

Y

A

V

F

K

K

E

E

V

V

K

N

H

D

L

M

A

L

V

L

Q

E

E

N

G

G

M

I

S

K

D

-

V

I

K

P

V

F

L
x
A

A

C

G
|
G

A
x
G

A

A

L

V

R

N

Q

Q

A

-

E

-

P

-

E

-

Q

-

L

-

N

-

V

-

D

D

F

F

V

V

A

S

Q

Q

N

-

V

-

F

F

H

A

L

L

L

G

G

V

Y

Y

L
x
G

K
x
E

E
|
E

E
x
A

D

A

E

D

A

A

K

P

R

K

active site: D134 (= D107), H136 (= H109), T187 (= T160)
binding 5-hydroxybenzimidazolylcob(iii)amide: G133 (= G106), D134 (= D107), V135 (≠ M108), H136 (= H109), D137 (= D110), I138 (≠ L111), G139 (= G112), V143 (= V116), T179 (≠ G152), T181 (≠ S154), L183 (= L156), M184 (≠ I157), T185 (= T158), A208 (≠ L182), G210 (= G184), G211 (= G185), G212 (≠ A186), G228 (≠ L212), E229 (≠ K213), E230 (= E214), A231 (≠ E215)

Q46EH4 Methanol--corrinoid protein; Methanol:corrinoid methyltransferase 1 subunit of 27 kDa; MT1 subunit 27 kDa from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
30% identity, 72% coverage: 63:220/220 of query aligns to 95:236/258 of Q46EH4

query
sites
Q46EH4

L

L

L

P

E

N

I

V

L

M

A

S

G

A

R

D

A

A

M

M

M

L

D

E

V

G

M

I

E

E

Q

-

V

-

S

-

K

-

H

Y

V

C

C

K

F

E

S

N

T

S

L

G

A

A

M

T

G

P

K

K

E

T

Q

K

P

G

V

T

F

V

V

V

I

C

G
x
H

T

V

I

A

K

E

G

G

D

D

M

V

H
|
H

D

D

L

I

G

G

K

K

N

N

I

I

V

V

S

T

M

A

M

L

L

L

K

R

V

A

A

N

G

G

Y

Y

R

N

V

V

I

V

D

D

L

L

G

G

K

R

D

D

V

V

E

P

P

A

A

E

K

E

F

V

I

L

E

A

T

A

A

V

A

Q

A

K

E

E

Q

K

A

P

R

I

Y

M

I

L

G

T

I

G

S

T

S

A

L

L

I

M

T

T

I

M

P

Y

Y

A

V

F

K

K

E

E

V

V

K

N

H

D

L

M

A

L

V

L

Q

E

E

N

G

G

M

I

S

K

D

-

V

I

K

P

V

F

L

A

A

C

G

G

A

G

A

A

L

V

R

N

Q

Q

A

-

E

-

P

-

E

-

Q

-

L

-

N

-

V

-

D

D

F

F

V

V

A

S

Q

Q

N

-

V

-

F

F

H

A

L

L

L

G

G

V

Y

Y

L

G

K

E

E

E

E

A

D

A

E

D

A

A

K

P

R

K

H129 (≠ G102) mutation to K: Does not affect cobalamin-binding.
H136 (= H109) mutation H->G,K: Abolishes cobalamin-binding.

Sites not aligning to the query:

256:258 HKH→KKK: Does not affect cobalamin-binding.

8sseA Methionine synthase, c-terminal fragment, cobalamin and reactivation domains from thermus thermophilus hb8 (see paper)
32% identity, 59% coverage: 79:207/220 of query aligns to 66:192/507 of 8sseA

query
sites
8sseA

Q

E

V

V

V

M

S

K

K

R

H

A

V

V

C

A

F

Y

S

L

T

E

L

P

A

H

M

M

G

E

K

K

E

E

-

G

Q

K

P

G

V

T

F

L

V

V

I

L

G

A

T

T

I

V

K

K

G

G

D

D

M

V

H
|
H

D

D

L

I

G
|
G

K

K

N

N

I

L

V
|
V

S

D

M

I

L

L

K

S

V

N

A

N

G

G

Y

Y

R

R

V

V

I

V

D

N

L

L

G

G

K

I

D

K

V

V

E

P

P

I

A

E

K

E

F

I

I

L

E

K

T

A

A

V

A

E

A

A

E

H

Q

K

A

P

R

H

Y

A

I

V

G

G

I

M

S
|
S

S

G

L

L

I
x
L

T
x
V

T

K

T

S

I

T

P

L

Y

V

V

M

K

K

E

E

V

N

K

L

H

E

L

Y

A

M

V

R

Q

D

E

R

G

G

M

Y

S

T

D

-

V

L

K

P

V

V

L
x
I

A

L

G
|
G

A
|
A

A

A

L

L

R

T

Q

R

A

S

E

Y

P

V

E

E

Q

E

L

L

N

R

V

A

D

-

F

-

V

I

A

Y

Q

P

N

N

V

V

F

Y

H

Y

binding cobalamin: H97 (= H109), G100 (= G112), V104 (= V116), S142 (= S154), L145 (≠ I157), V146 (≠ T158), I169 (≠ L182), G171 (= G184), G172 (= G185), A173 (= A186)

Sites not aligning to the query:

binding cobalamin: 405, 409, 451, 452, 453, 454, 463, 485, 488, 490, 492

3ezxA Structure of methanosarcina barkeri monomethylamine corrinoid protein
28% identity, 78% coverage: 26:196/220 of query aligns to 24:192/212 of 3ezxA

query
sites
3ezxA

L

L

A

C

R

K

R

E

L

A

L

L

D

A

H

A

G

G

L

V

D

P

L

A

E

L

T

D

V

I

E

T

E

K

G

G

L

L

T

S

K

V

A

G

L
x
M

E

K

S

I

L

V

S

G

D

D

K

K

C
x
F

G

E

N

A

D

A

Q

E

F

I

S

F

L

L

L

P

E

Q

I

I

L

M

A

S

G

G

R

K

A

A

M

M

M

S

D

N

V

A

M

M

E

E

Q

-

V

-

S

-

K

-

H

-

V

V

C

L

F

T

S

P

T

E

L

L

A

E

M

K

G

N

K

K

E

K

Q

E

P

A

V

G

F

L

V

A

I

I

G

T

T

F

I

V

-

A

K

E

G

G

D
|
D

M
x
I

H
|
H

D
|
D

L
x
I

G

G

K

H

N

R

I

L

V
|
V

S

T

M

T

M

M

L

L

K

G

V

A

A

N

G

G

Y

F

R

Q

V

I

I

V

D

D

L

L

G

G

K

V

D

D

V

V

E

L

P

N

A

E

K

N

F

V

I

V

E

E

T

E

A

A

-

K

-

H

-

K

A

G

E

E

Q

K

A

V

R

L

Y

L

I
x
V

G

G

I

-

S
|
S

S

A

L
|
L

I
x
M

T
|
T

T

T

T
x
S

I

M

P

L

Y

G

V

Q

K

K

E

D

V

L

K

M

H

D

L

R

A

L

V

N

Q

E

E

E

G

K

M

L

S

R

D

D

-

S

V

V

K

K

V

C

L
x
M

A

F

G
|
G

A

A

A

P

L

V

R

S

Q

D

A

K

E

W

P

I

E

E

Q

E

L

I

active site: D100 (= D107), H102 (= H109), S155 (≠ T160)
binding 5-hydroxybenzimidazolylcobamide: M47 (≠ L49), F54 (≠ C56), D100 (= D107), I101 (≠ M108), H102 (= H109), D103 (= D110), I104 (≠ L111), V109 (= V116), V147 (≠ I151), S149 (= S154), L151 (= L156), M152 (≠ I157), T153 (= T158), M178 (≠ L182), G180 (= G184), G181 (= G185)

Sites not aligning to the query:

binding 5-hydroxybenzimidazolylcobamide: 198, 200

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
40% identity, 41% coverage: 96:186/220 of query aligns to 772:861/1265 of Q99707

query
sites
Q99707

Q

Q

P

G

V

T

F

I

V

V

I

L

G

A

T

T

I

V

K

K

G

G

D

D

M

V

H

H

D

D

L

I

G

G

K

K

N

N

I

I

V

V

S

G

M

V

L

L

K

G

V

C

A

N

G

N

Y

F

R

R

V

V

I

I

D

D

L

L

G

G

K

V

D

M

V

T

E

P

P

C

A

D

K

K

F

I

I

L

E

K

T

A

A

A

A

L

A

D

E

H

Q

K

A

A

R

D

Y

I

I

I

G

G

I

L

S

S

S

G

L

L

I

I

T

T

T

P

T

S

-

L

-

D

-

E

-

M

I

I

P

F

Y

V

V

A

K

K

E

E

V

M

K

E

H

R

L

L

A

A

V

I

Q

R

E

-

G

-

M

-

S

-

D

-

V

I

K

P

V

L

L

L

A

I

G

G

A

A

Sites not aligning to the query:

61 natural variant: R -> K
255 C → Y: in dbSNP:rs1140598
382:384 binding
449 binding
470 binding
537 binding
579 binding
585 binding
591 binding
919 D → G: in dbSNP:rs1805087
963 D→E: Decreases binding to MTRR; when associated with N-1071.
1071 K→N: Decreases binding to MTRR; when associated with E-963.

4jgiB 1.5 angstrom crystal structure of a novel cobalamin-binding protein from desulfitobacterium hafniense dcb-2 (see paper)
26% identity, 81% coverage: 26:204/220 of query aligns to 27:193/206 of 4jgiB

query
sites
4jgiB

L

M

A

A

R

K

R

A

L

D

L

A

D

D

H

A

G

A

L

Q

D

E

L

L

E

I

T

K

V

A

V

F

E

Q

G

G

L

M

T

T

K

K

A

V

L

G

E

E

S

R

L

F

S

D

D

-

K

-

C

-

G

-

N

S

D

G

Q

E

F

Y

S

F

L

I

L

G

E

D

I
x
L

L

I

L

F

A

A

G

G

-

E

-

I

-

L

R

Q

A

A

M

A

M

M

D

D

V

K

M

L

E

K

Q

P

V

A

V

L

S

E

K

K

H

-

V

-

C

-

F

-

S

-

T

-

L

-

A

-

M

-

G

-

K

-

E

-

Q

R

P

A

V

K

F

I

V

V

I

L

G

A

T

T

I

V

K

E

G

G

D
|
D

M
x
L

H
|
H

D
|
D

L
x
I

G
|
G

K

K

N

N

I

I

V
x
F

S

R

M

T

M

M

L

A

K

E

V

A

A

S

G

G

Y

F

R

E

V

V

I

F

D

D

L

L

G

G

K

I

D

D

V

V

E

-

P

P

A

V

K

K

F

I

I

I

E

V

T

D

A

K

A

V

A

K

E

E

-

V

Q

N

A

P

R

E

Y

I

I

V

G
|
G

I

L

S
|
S

S

G

L

V

I
x
L

T

T

T

L

T
x
A

I

L

P

D

Y

S

V

M

K

R

E

E

V

T

K

V

H

D

L

A

A

L

V

K

Q

A

E

E

G

G

M

L

-

R

S

N

D

D

V

L

K

K

V

V

L

I

A

I

G
|
G

A
x
V

A

P

L

V

R

N

Q

E

A

N

E

V

P

C

E

Q

Q

R

L

V

N

G

V

A

D

D

F

D

V

F

A
x
S

Q
x
T

N
|
N

active site: D95 (= D107), H97 (= H109), A148 (≠ T160)
binding co-methylcobalamin: L63 (≠ I66), D95 (= D107), L96 (≠ M108), H97 (= H109), D98 (= D110), I99 (≠ L111), G100 (= G112), F104 (≠ V116), G140 (= G152), S142 (= S154), L145 (≠ I157), G173 (= G184), G174 (= G185), V175 (≠ A186), S191 (≠ A202), T192 (≠ Q203), N193 (= N204)

Sites not aligning to the query:

binding co-methylcobalamin: 194

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
27% identity, 80% coverage: 40:214/220 of query aligns to 659:831/841 of 8g3hA

query
sites
8g3hA

V

I

E

N

E

G

G

P

L

L

T

L

K

E

A

A

L

M

E

K

S

E

L

V

S

G

D

E

K

L

C
x
F

G

G

N

A

D

G

Q

K

F

M

S

Q

L

L

L

P

E

F

I
x
V

L

L

L

Q

A

A

G

A

R

-

A

-

M

-

D

E

V

V

M

M

E
x
K

Q

Q

V

A

V

V

S

A

-

Y

-

L

-

E

K

P

H

H

V

M

C

E

F

K

S

K

T

G

L

E

A

G

M

K

G

G

K

K

E

-

Q

-

P

-

V

-

F

L

V

V

I

L

G

A

T

T

I

V

K

K

G
|
G

D

D

M
x
V

H
|
H

D
|
D

L
x
I

G
|
G

K

K

N

N

I

L

V
|
V

S

D

M

I

L

L

K

T

V

N

A

N

G

G

Y

Y

R

T

V

V

I

Y

D

N

L

L

G

G

K

I

D

K

V

V

E

P

P

I

A

E

K

E

F

M

I

L

E

K

T

A

A

V

A

D

A

E

E

V

Q

K

A

P

R

H

Y

A

I

L

G

G

I
x
M

S
|
S

S

G

L
|
L

I

L

T
x
V

T

K

T

S

I

T

P

Q

Y

V

V

M

K

K

E

E

V

N

K

L

H

E

L

Y

A

M

V

R

Q

A

E

R

G

G

M

Y

S

T

D

-

V

L

K

P

V

V

L
x
I

A
x
L

G
|
G

A
|
A

A

A

L

L

R

T

Q

R

A

A

E

Y

P

V

E

E

Q

E

L

L

N

R

V

S

D

I

-

Y

-

P

-

E

-

V

F

Y

V
x
Y

A
|
A

Q
x
E

N
x
D

V

A

F

F

H

E

L

G

L

L

G

A

Y

L

L

M

K

E

E

E

binding cobalamin: F675 (≠ C56), V685 (≠ I66), K693 (≠ E78), G720 (= G106), V722 (≠ M108), H723 (= H109), D724 (= D110), I725 (≠ L111), G726 (= G112), V730 (= V116), M767 (≠ I153), S768 (= S154), L770 (= L156), V772 (≠ T158), I795 (≠ L182), L796 (≠ A183), G797 (= G184), G798 (= G185), A799 (= A186), Y818 (≠ V201), A819 (= A202), E820 (≠ Q203), D821 (≠ N204)

Sites not aligning to the query:

binding cobalamin: 328, 330, 331

8j2xA Saccharothrix syringae photocobilins protein, light state (see paper)
25% identity, 72% coverage: 10:167/220 of query aligns to 10:161/337 of 8j2xA

query
sites
8j2xA

E

E

L

L

V

M

V

A

A

A

I

L

T

A

E

E

G

G

N

D

N

E

L

H

E

A

A

A

V

V

A

D

L

L

A

A

R

M

R

R

L

L

D

D

H

G

G

G

L

V

D

P

L

A

E

D

T

V

V

V

V

L

E

L

E

E

G

L

L

V

T
x
A

K

D

A

A

L
x
Q

E

V

S

E

L

I

S
x
G

D

V

K

L

C
x
W

G
x
Q

N

A

D

N

Q

R

F

W

S

S

L

V

L

A

E

Q

I

-

L

-

A

-

G

E

R

H

A

A

M

A

M

T

D

A

V

I

M

S

E

E

Q

R

V

V

V

I

S

A

K

A

H

V

V

G

C

D

F

R

S

A

T

A

L

A

A

A

M

P

G

T

K

R

E

G

Q

H

P

-

V

-

F

V

V

V

I

V

G

A

T

C

I

L

K

D

G

G

D

E

M
x
W

H
|
H

D
x
A

L
|
L

G
x
P

K

A

N

R

I

I

V
|
V

S

A

M

E

M

V

L

L

K

R

V

G

A

R

G

G

Y

W

R

R

V

V

I

T

D

F

L

L

G

G

K

A

D

S

V

V

E

P

P

A

A

A

K

H

F

L

I

V

E

P

T

Y

A

L

A

E

E

H

Q

G

A

P

R

D

Y

A

I

V

G
x
A

I
x
L

S
|
S

S

C

L

T

I
x
L

T

P

T

R

T

G

I

L

P

P

Y

R

V

A

K

D

E

Q

V

V

binding cobalamin: A46 (≠ T46), Q49 (≠ L49), G53 (≠ S53), W56 (≠ C56), Q57 (≠ G57), W102 (≠ M108), H103 (= H109), A104 (≠ D110), L105 (= L111), P106 (≠ G112), V110 (= V116), A146 (≠ G152), L147 (≠ I153), S148 (= S154), L151 (≠ I157)
binding biliverdine ix alpha: W56 (≠ C56)

Sites not aligning to the query:

binding cobalamin: 173, 174, 175, 176, 195, 196, 197, 198, 287
binding biliverdine ix alpha: 250, 253, 254, 257, 258, 261, 264, 287, 292, 295, 297, 301

Query Sequence

>WP_013841550.1 NCBI__GCF_000215085.1:WP_013841550.1
MTEKIPNTRELVVAITEGNNLEAVALARRLLDHGLDLETVVEEGLTKALESLSDKCGNDQ
FSLLEILLAGRAMMDVMEQVVSKHVCFSTLAMGKEQPVFVIGTIKGDMHDLGKNIVSMML
KVAGYRVIDLGKDVEPAKFIETAAAEQARYIGISSLITTTIPYVKEVKHLAVQEGMSDVK
VLAGGAALRQAEPEQLNVDFVAQNVFHLLGYLKEEDEAKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory