SitesBLAST

P11043 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EC 2.5.1.19 from Petunia hybrida (Petunia) (see paper)
30% identity, 26% coverage: 326:446/459 of query aligns to 394:512/516 of P11043

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P11043

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173 G→A: This mutant becomes resistant to glyphosate due to a lower affinity. Shows a slight reduction in EPSP synthase activity.

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
26% identity, 21% coverage: 350:446/459 of query aligns to 739:838/1583 of P07547

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Sites not aligning to the query:

44:46 binding NAD(+)
81:84 binding NAD(+)
114:116 binding NAD(+)
119 binding NAD(+)
139:140 binding NAD(+)
161 binding NAD(+)
179:182 binding NAD(+)
190 binding NAD(+)
194 binding Zn(2+)
271 binding Zn(2+)
287 binding Zn(2+)

Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
26% identity, 43% coverage: 255:451/459 of query aligns to 231:429/438 of Q83E11

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Q83E11

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D315 (≠ R331) binding 3-phosphoshikimate
K342 (= K362) binding 3-phosphoshikimate
R346 (= R366) binding phosphoenolpyruvate
R387 (= R409) binding phosphoenolpyruvate

Sites not aligning to the query:

21 binding phosphoenolpyruvate
22 binding 3-phosphoshikimate
26 binding 3-phosphoshikimate
93:96 Phosphoenolpyruvate
95 binding phosphoenolpyruvate
96 binding phosphoenolpyruvate
123 binding phosphoenolpyruvate
167 binding 3-phosphoshikimate
168 binding 3-phosphoshikimate
169 binding 3-phosphoshikimate; binding phosphoenolpyruvate

3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
27% identity, 34% coverage: 296:451/459 of query aligns to 273:431/440 of 3slhD

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active site: D317 (≠ R331), E345 (= E363), H388 (= H408), R389 (= R409), T415 (≠ K435)
binding glyphosate: D317 (≠ R331), E345 (= E363), R348 (= R366), H388 (= H408), R389 (= R409)
binding shikimate-3-phosphate: D317 (≠ R331), K344 (= K362)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D317 (≠ R331), K344 (= K362)

Sites not aligning to the query:

active site: 23, 24, 50, 95, 125
binding glyphosate: 23, 97, 98, 125, 171
binding shikimate-3-phosphate: 24, 28, 169, 171, 196
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 24, 28, 98, 171, 196

4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
26% identity, 43% coverage: 255:451/459 of query aligns to 231:427/434 of 4egrA

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4egrA

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active site: D313 (≠ R331), E341 (= E363), H384 (= H408), R385 (= R409), T411 (≠ K435)
binding phosphoenolpyruvate: D313 (≠ R331), E341 (= E363), R344 (= R366), R385 (= R409)

Sites not aligning to the query:

active site: 23, 24, 50, 95, 125
binding phosphoenolpyruvate: 23, 97, 98, 125

Query Sequence

>WP_014025668.1 NCBI__GCF_000223395.1:WP_014025668.1
MPDSVMGRALVADPHVVVVTGFEPKSSVVEIKAPPSKSFYYRLAGLLLAGRGCVRNVPDC
EDADAATDIVLAAGGVVVSDGRVCLDYFRESETYDVDVYCSGGALRITLVALLAAAPIGS
RISVHVCKRLFRRLEEGTLSLWKQFGEARLDPGSNVINIVKTREPQMLRVASTKSSQPIS
GALIAAAILSALRGGDPVKVEGYTGVSSGHIYETLESLRLAGFTLEDIRIDERGDGIAIH
ALVSGVERRFVLWTPGDWGLASLVAPLASTGVRLIIRGLWRPWAGPGDHYASFYYKILGF
DSKILEHDDSAEWDIEPFSARSADNITITVRREPDVAVSVAYAASALGVSVKIAGVHHLV
LKESNRLESVTASLRSLGYVAYHTNDDIIVAGRAVKPSFVNIECPDDHRIAMGTAVLAAT
SGVVARISNATCTSKSWRGFWSTLQEAGVRIFFEGGKLE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory