SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_014026286.1 NCBI__GCF_000223395.1:WP_014026286.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

Q4JAP9 Alpha-aminoadipate--LysW ligase LysX; AAA--LysW ligase LysX; EC 6.3.2.43 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
46% identity, 93% coverage: 11:275/284 of query aligns to 2:267/276 of Q4JAP9

query
sites
Q4JAP9

R

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NT 253:254 (= NT 261:262) mutation to GF: Alters substrate specificity, so that glutamate is preferred over alpha-aminoadipate.

5k2mI Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
50% identity, 100% coverage: 1:283/284 of query aligns to 1:272/273 of 5k2mI

query
sites
5k2mI

M

M

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K

binding adenosine-5'-diphosphate: K83 (= K90), V120 (= V128), K122 (= K130), S127 (= S135), L132 (≠ V140), Q162 (= Q170), V165 (= V173), K167 (= K175), D171 (= D179), W192 (= W201), I193 (≠ K202), T194 (= T203), N195 (= N204), F231 (= F241), N240 (= N251), E241 (= E252)
binding 2-aminohexanedioic acid: R173 (= R181), N195 (= N204), T196 (= T205), A197 (= A206), E247 (≠ D258), F248 (= F259), N250 (= N261)
binding : V51 (= V58), S52 (= S59), H53 (≠ Y60), F54 (= F61), F125 (≠ I133), W128 (= W136), R130 (= R138), N153 (≠ P161), Y156 (= Y164), N245 (= N256)

5k2mG Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
50% identity, 100% coverage: 1:283/284 of query aligns to 1:272/273 of 5k2mG

query
sites
5k2mG

M

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K

binding adenosine-5'-diphosphate: K83 (= K90), V120 (= V128), K122 (= K130), S127 (= S135), G129 (= G137), Q162 (= Q170), V165 (= V173), K167 (= K175), D171 (= D179), W192 (= W201), T194 (= T203), N195 (= N204), F231 (= F241), N240 (= N251), E241 (= E252)
binding magnesium ion: D229 (= D239), E241 (= E252)
binding : V51 (= V58), S52 (= S59), H53 (≠ Y60), F54 (= F61), F125 (≠ I133), S127 (= S135), W128 (= W136), R130 (= R138), Y156 (= Y164), R173 (= R181), R187 (= R195), T196 (= T205), A197 (= A206), N243 (= N254), P244 (≠ A255), N245 (= N256), E247 (≠ D258), F248 (= F259), N250 (= N261), A251 (≠ T262)

3vpdA Lysx from thermus thermophilus complexed with amp-pnp (see paper)
47% identity, 99% coverage: 3:283/284 of query aligns to 2:280/281 of 3vpdA

query
sites
3vpdA

I

L

A

A

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L

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S

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R

binding phosphoaminophosphonic acid-adenylate ester: V127 (= V128), K129 (= K130), W135 (= W136), G136 (= G137), L139 (≠ V140), Q169 (= Q170), V172 (= V173), K174 (= K175), D178 (= D179), R194 (= R195), W199 (= W201), T201 (= T203), F239 (= F241), E249 (= E252)
binding butanoic acid: F256 (= F259), K257 (= K260), N258 (= N261), S259 (≠ T262)

3vpbB Argx from sulfolobus tokodaii complexed with lysw/glu/adp/mg/zn/sulfate (see paper)
38% identity, 98% coverage: 1:277/284 of query aligns to 1:275/282 of 3vpbB

query
sites
3vpbB

M

M

R

R

I

V

A

V

I

L

V

I

Y

V

D

D

H

I

P

V

R

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Q

E

E

K

K

R

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L

I

A

A

E

-

A

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K

K

A

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L

G

E

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E

E

N

P

K

V

V

L

Q

F

Y

N

D

I

I

D

I

S

N

L

V

L

A

F

Q

R

E

L

P

D

L

S

P

L

F

E

N

R

K

I

A

L

L

G

G

D

R

V

Y

D

D

V

V

V

A

L

I

Q

I

R

R

A

P

V

V

S

S

Y

M

F

Y

K

R

A

A

L

L

E

Y

S

S

T

S

R

A

I

V

L

L

E

E

A

A

G

G

Y

V

T

H

V

T

I

I

N

N

N

S

S

S

L

D

V

V

Q

I

L

N

N

V

C

C

G

G

D

D

K
|
K

L

I

L

L

T

T

T

Y

I

S

L

K

L

L

A

Y

K

R

H

E

G

G

V

I

P

P

T

I

P

P

R

D

A

S

Y

I

A

I

A

A

F

L

S

S

R

A

D

E

T

A

A

A

V

L

R

K

A

A

A

Y

E

E

E

Q

L

R

G

G

Y

F

P

P

V

L

V
x
I

V

D

K
|
K

P

P

V

P

I

I

G

G

S
|
S

W

W

G
|
G

R

R

L

L

V
|
V

A

S

R

L

A

I

D

R

S

D

R

V

E

F

S

E

L

G

E

K

A

T

V

I

I

I

E

E

H

H

R

R

E

E

V

L

L

M

G

G

P

N

A

S

Y

A

Y

L

K

K

V

A

H

H

Y

I

V

V

Q
|
Q

E

E

Y
|
Y

V
x
I

R

Q

K

Y

P

K

L

G

R

R

D
|
D

I

I

R

R

V

C

F

I

V

A

I

I

G

G

D

E

E

E

V

L

P

L

V

G

A

C

I
x
Y

Y

A

R
|
R

-

N

V

I

N

P

E

P

R

N

H

E

W

W

K
x
R

T
x
A

N
|
N

T
x
V

A
|
A

L

L

G

G

A

G

K

T

A

P

E

S

P

N

A

I

P

E

V

V

T

D

P

E

E

K

L

L

R

K

E

E

L

T

A

V

L

V

R

K

A

A

A

V

K

S

A

I

V

V

G

H

G

G

G

E

V

F

L

V

G

S

I

I

D
|
D

V

I

F

L

E

E

D

H

P

P

E

N

R

K

G

G

L

Y

L

V

V

V

N
|
N

E
|
E

I

L

N
|
N

A

D

N

V

S

P

D
x
E

F

F

K

K

N
x
G

T

F

E

M

R

V

V

A

T

T

G

N

F

I

N

N

M

V

A

A

R

Q

A

K

I

L

V

V

E

E

Y

Y

binding adenosine-5'-diphosphate: K87 (= K90), I125 (≠ V128), K127 (= K130), S132 (= S135), G134 (= G137), V137 (= V140), Q167 (= Q170), Y169 (= Y172), I170 (≠ V173), D176 (= D179), R200 (≠ K202), A201 (≠ T203), N202 (= N204), N249 (= N251), E250 (= E252)
binding glutamic acid: Y190 (≠ I193), R192 (= R195), N202 (= N204), V203 (≠ T205), A204 (= A206), E256 (≠ D258), G259 (≠ N261)
binding magnesium ion: D237 (= D239), E250 (= E252)
binding zinc ion: E250 (= E252), N252 (= N254)

Q970U6 Glutamate--LysW ligase ArgX; EC 6.3.2.- from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
38% identity, 98% coverage: 1:277/284 of query aligns to 1:275/282 of Q970U6

query
sites
Q970U6

M

M

R

R

I

V

A

V

I

L

V

I

Y

V

D

D

H

I

P

V

R

R

V

Q

E

E

K

K

R

L

L

I

A

A

E

-

A

-

R

K

K

A

L

L

G

E

H

E

E

N

P

K

V

V

L

Q

F

Y

N

D

I

I

D

I

S

N

L

V

L

A

F

Q

R

E

L

P

D

L

S

P

L

F

E

N

R

K

I

A

L

L

G

G

D

R

V

Y

D

D

V

V

V

A

L

I

Q

I

R

R

A

P

V

V

S

S

Y

M

F

Y

K

R

A

A

L

L

E

Y

S

S

T

S

R

A

I

V

L

L

E

E

A

A

G

G

Y

V

T

H

V

T

I

I

N

N

N

S

S

S

L

D

V

V

Q

I

L

N

N

V

C

C

G

G

D

D

K

K

L

I

L

L

T

T

T

Y

I

S

L

K

L

L

A

Y

K

R

H

E

G

G

V

I

P

P

T

I

P

P

R

D

A

S

Y

I

A

I

A

A

F

L

S

S

R

A

D

E

T

A

A

A

V

L

R

K

A

A

A

Y

E

E

E

Q

L

R

G

G

Y

F

P

P

V

L

V

I

V

D

K

K

P

P

V

P

I

I

G

G

S

S

W

W

G

G

R

R

L

L

V

V

A

S

R

L

A

I

D

R

S

D

R

V

E

F

S

E

L

G

E

K

A

T

V

I

I

I

E

E

H

H

R

R

E

E

V

L

L

M

G

G

P

N

A

S

Y

A

Y

L

K

K

V

A

H

H

Y

I

V

V

Q

Q

E

E

Y

Y

V

I

R

Q

K

Y

P

K

L

G

R

R

D

D

I

I

R

R

V

C

F

I

V

A

I

I

G

G

D

E

E

E

V

L

P

L

V

G

A

C

I

Y

Y

A

R

R

-

N

V

I

N

P

E

P

R

N

H

E

W

W

K

R

T

A

N

N

T

V

A

A

L

L

G

G

A

G

K

T

A

P

E

S

P

N

A

I

P

E

V

V

T

D

P

E

E

K

L

L

R

K

E

E

L

T

A

V

L

V

R

K

A

A

A

V

K

S

A

I

V

V

G

H

G

G

G

E

V

F

L

V

G

S

I

I

D
|
D

V

I

F

L

E

E

D

H

P

P

E

N

R

K

G

G

L

Y

L

V

V

V

N

N

E
|
E

I

L

N
|
N

A

D

N

V

S

P

D

E

F

F

K

K

N

G

T

F

E

M

R

V

V

A

T

T

G

N

F

I

N

N

M

V

A

A

R

Q

A

K

I

L

V

V

E

E

Y

Y

D237 (= D239) binding Mg(2+)
E250 (= E252) binding Mg(2+); binding Mg(2+)
N252 (= N254) binding Mg(2+)

1uc9A Crystal structure of a lysine biosynthesis enzyme, lysx, from thermus thermophilus hb8 (see paper)
40% identity, 99% coverage: 3:283/284 of query aligns to 2:256/256 of 1uc9A

query
sites
1uc9A

I

L

A

A

I

I

V

L

Y

Y

D

D

H

R

P

I

R

R

V

P

E

D

E

E

K

R

R

M

L

L

A

F

E

E

E

R

A

A

R

E

K

A

L

L

G

G

H

L

E

P

P

Y

V

K

L

K

F

V

N

Y

I

V

D

P

S

A

L

L

L

P

F

M

R

V

L

L

D

G

S

E

L

R

E

P

R

K

I

E

L

L

G

E

D

G

V

V

D

T

V

V

V

A

L

L

Q

E

R

R

A

C

V

V

S

S

Y

Q

F

S

K

R

A

G

L

L

E

A

S

A

T

A

R

R

I

Y

L

L

E

T

A

A

A

L

G

G

Y

I

T

P

V

V

I

V

N

N

N

R

S

P

L

E

V

V

Q

I

L

E

N

A

C

C

G

G

D

D

K

K

L

W

L

A

T

T

T

S

I

V

L

A

L

L

A

A

K

K

H

A

G

G

V

L

P

P

T

Q

P

P

R

K

A

T

Y

A

A

L

A

A

F

T

S

D

R

R

D

E

T

E

A

A

V

L

R

R

A

L

A

M

E

E

E

A

L

F

G

G

Y

Y

P

P

V

V

V

L

K
|
K

P

P

V

V

I

I

G

G

S

A

W

A

G

A

R

A

L

A

V

A

A

A

R

A

A

A

D

A

S

A

R

A

E

A

S

A

L

A

E

A

A

G

V

F

I

-

E

-

H

-

R

-

E

-

V

-

L

-

G

-

P

-

A

-

Y

Q

Y

H

K

Q

V

L

H

F

Y

Y

V

I

Q

Q

E

E

Y
|
Y

V
|
V

R

E

K

K

P

P

L

G

R

R

D
|
D

I

I

R

R

V

V

F

F

V

V

I

V

G

G

D

E

E

R

V

A

P

I

V

A

A

A

I

I

Y

Y

R
|
R

V

-

N

-

E

-

R

-

H

-

W

-

K

-

T

-

N

-

T

-

A

-

L

-

G

-

A

-

K

-

A

A

E

E

P

N

A

C

P

P

V

L

T

T

P

E

E

E

L

V

R

A

E

R

L

L

A

S

L

V

R

K

A

A

K

E

A

A

V

V

G

G

V

V

L

V

G

A

I

V

D

D

V

L

F
|
F

E

E

D

S

P

-

E

E

R

R

G

G

L

L

V

V

N
|
N

E

E

I

V

N

N

A

H

N

T

S

M

D

E

F

F

K

K

N

N

T

S

E

V

R

H

V

T

T

T

G

G

F

V

N

D

M

I

A

P

R

G

A

E

I

I

V

L

E

K

Y

Y

A

A

V

W

S

S

V

L

A

A

K

S

binding adenosine-5'-diphosphate: K129 (= K130), Y161 (= Y172), V162 (= V173), D168 (= D179), R184 (= R195), F215 (= F241), N224 (= N251)

5zctH The crystal structure of the poly-alpha-l-glutamate peptides synthetase rimk at 2.05 angstrom resolution. (see paper)
32% identity, 98% coverage: 1:277/284 of query aligns to 1:285/292 of 5zctH

query
sites
5zctH

M

M

R

K

I

I

A

A

I

I

V

L

Y

S

-

R

D

D

H

G

P

T

R

L

V

Y

E

S

E

C

K

K

R

R

L

L

A

R

E

E

E

A

A

A

R

I

K

Q

L

R

G

G

H

H

-

L

-

V

-

E

-

I

-

L

E

D

P

P

V

L

-

S

-

C

L

Y

F

M

N

N

I

I

D

N

S

P

L

A

F

S

R

S

L

I

D

H

S

Y

L

K

E

G

R

R

I

K

L

L

G

P

D

H

V

F

D

D

V

A

V

V

L

I

Q

P

R

R

-

I

-

G

-

T

A

A

V

I

S

T

Y

F

F

Y

K

-

A

G

L

T

E

A

S

A

T

L

R

R

I

Q

L

F

E

E

A

M

A

L

G

G

Y

S

T

Y

V

P

I

L

N

N

N

E

S

S

L

V

V

A

Q

I

L

A

N

R

C

A

G

R

D

D

K

K

L

L

L

R

T

S

T

M

I

Q

L

L

A

A

K

R

H

Q

G

G

V

I

P

D

T

L

P

P

R

V

A

T

Y

G

A

I

A

A

F

H

S

S

R

P

D

D

T

D

A

T

V

S

R

D

A

L

A

I

E

D

E

M

L

V

G

G

-

G

Y

A

P

P

V

L

V
|
V

V

V

K
|
K

P

L

V

V

I

E

G

G

S

T

W

Q

G

G

R

I

L

G

V

V

A

V

R

L

A

A

D

E

S

T

R

R

E

Q

S

A

L

A

E

E

A

S

V

V

I

I

E

D

H

A

R

F

E

R

V

G

L

L

G

N

P

A

A

H

Y

I

Y

L

K

-

V

-

H

-

Y

-

V

V

Q
|
Q

E

E

Y
|
Y

V
x
I

R

K

K

E

P

A

L

Q

R

G

-

C

D
|
D

I

I

R

R

V

C

F

L

V

V

I

V

G

G

D

D

E

E

V

V

P

V

V

A

A

A

I

I

-

E

Y

R

R

R

V

A

N

K

E

E

R

G

H

D

W
x
F

K
x
R

T
x
S

N
|
N

T

L

A

H

L
x
R

G

G

A

G

K

A

A

A

E

S

P

V

A

A

P

S

V

I

T

T

P

P

E

Q

L

E

R

R

E

E

L

I

A

A

L

I

R

K

A

A

K

R

A

T

V

M

G

A

G

L

G

D

V

V

L

A

G

G

I

V

D
|
D

V

I

F
x
L

E

R

D

-

P

A

E

N

R

R

G

G

L

P

L

L

V

V

N
x
M

E
|
E

I

V

N

N

A

A

N

S

S

P

D

G

F

L

K

E

N

G

T

I

E

E

R

K

V

T

T

T

G

G

F

I

N

D

M

I

A

A

R

G

A

K

I

M

V

I

E

R

Y

W

binding phosphoaminophosphonic acid-adenylate ester: V139 (= V128), K141 (= K130), Q177 (= Q170), Y179 (= Y172), I180 (≠ V173), D187 (= D179), F210 (≠ W201), R211 (≠ K202), S212 (≠ T203), N213 (= N204), R216 (≠ L207), L250 (≠ F241), M259 (≠ N251), E260 (= E252)
binding magnesium ion: D248 (= D239), E260 (= E252)

5zctA The crystal structure of the poly-alpha-l-glutamate peptides synthetase rimk at 2.05 angstrom resolution. (see paper)
32% identity, 98% coverage: 1:277/284 of query aligns to 1:285/292 of 5zctA

query
sites
5zctA

M

M

R

K

I

I

A

A

I

I

V

L

Y

S

-

R

D

D

H

G

P

T

R

L

V

Y

E

S

E

C

K

K

R

R

L

L

A

R

E

E

E

A

A

A

R

I

K

Q

L

R

G

G

H

H

-

L

-

V

-

E

-

I

-

L

E

D

P

P

V

L

-

S

-

C

L

Y

F

M

N

N

I

I

D

N

S

P

L

A

F

S

R

S

L

I

D

H

S

Y

L

K

E

G

R

R

I

K

L

L

G

P

D

H

V

F

D

D

V

A

V

V

L

I

Q

P

R

R

-

I

-

G

-

T

A

A

V

I

S

T

Y

F

F

Y

K

-

A

G

L

T

E

A

S

A

T

L

R

R

I

Q

L

F

E

E

A

M

A

L

G

G

Y

S

T

Y

V

P

I

L

N

N

N

E

S

S

L

V

V

A

Q

I

L

A

N

R

C

A

G

R

D

D

K
|
K

L

L

L

R

T

S

T

M

I

Q

L

L

A

A

K

R

H

Q

G

G

V

I

P

D

T

L

P

P

R

V

A

T

Y

G

A

I

A

A

F

H

S

S

R

P

D

D

T

D

A

T

V

S

R

D

A

L

A

I

E

D

E

M

L

V

G

G

-

G

Y

A

P

P

V

L

V

V

K
|
K

P

L

V

V

I

E

G

G

S
x
T

W
x
Q

G
|
G

R

I

L

G

V

V

A

V

R

L

A

A

D

E

S

T

R

R

E

Q

S

A

L

A

E

E

A

S

V

V

I

I

E

D

H

A

R

F

E

R

V

G

L

L

G

N

P

A

A

H

Y

I

Y

L

K

-

V

-

H

-

Y

-

V

V

Q
|
Q

E

E

Y
|
Y

V
x
I

R

K

K

E

P

A

L

Q

R

G

-

C

D

D

I

I

R

R

V

C

F

L

V

V

I

V

G

G

D

D

E

E

V

V

P

V

V

A

A

A

I

I

-

E

Y

R

R

R

V

A

N

K

E

E

R

G

H

D

W

F

K
x
R

T
x
S

N
|
N

T

L

A

H

L

R

G

G

A

G

K

A

A

A

E

S

P

V

A

A

P

S

V

I

T

T

P

P

E

Q

L

E

R

R

E

E

L

I

A

A

L

I

R

K

A

A

K

R

A

T

V

M

G

A

G

L

G

D

V

V

L

A

G

G

I

V

D

D

V

I

F
x
L

E

R

D

-

P

A

E

N

R

R

G

G

L

P

L

L

V

V

N
x
M

E

E

I

V

N

N

A

A

N

S

S

P

D

G

F

L

K

E

N

G

T

I

E

E

R

K

V

T

T

T

G

G

F

I

N

D

M

I

A

A

R

G

A

K

I

M

V

I

E

R

Y

W

binding phosphoaminophosphonic acid-adenylate ester: K100 (= K90), K141 (= K130), T146 (≠ S135), Q147 (≠ W136), G148 (= G137), Q177 (= Q170), Y179 (= Y172), I180 (≠ V173), R211 (≠ K202), S212 (≠ T203), N213 (= N204), L250 (≠ F241), M259 (≠ N251)

4iwxA Rimk structure at 2.85a (see paper)
32% identity, 98% coverage: 1:277/284 of query aligns to 3:287/294 of 4iwxA

query
sites
4iwxA

M

M

R

K

I

I

A

A

I

I

V

L

Y

S

-

R

D

D

H

G

P

T

R

L

V

Y

E

S

E

C

K

K

R

R

L

L

A

R

E

E

E

A

A

A

R

I

K

Q

L

R

G

G

H

H

-

L

-

V

-

E

-

I

-

L

E

D

P

P

V

L

-

S

-

C

L

Y

F

M

N

N

I

I

D

N

S

P

L

A

F

S

R

S

L

I

D

H

S

Y

L

K

E

G

R

R

I

K

L

L

G

P

D

H

V

F

D

D

V

A

V

V

L

I

Q

P

R

R

-

I

-

G

-

T

A

A

V

I

S

T

Y

F

F

Y

K

-

A

G

L

T

E

A

S

A

T

L

R

R

I

Q

L

F

E

E

A

M

A

L

G

G

Y

S

T

Y

V

P

I

L

N

N

N

E

S

S

L

V

V

A

Q

I

L

A

N
x
R

C

A

G

R

D

D

K

K

L

L

L
x
R

T

S

T

M

I
x
Q

L
|
L

L

L

A

A

K
x
R

H

Q

G

G

V

I

P

D

T

L

P

P

R

V

A

T

Y

G

A

I

A

A

F

H

S

S

R

P

D

D

T

D

A

T

V

S

R

D

A

L

A

I

E

D

E

M

L

V

G

G

-

G

Y

A

P

P

V

L

V
|
V

V

V

K
|
K

P

L

V

V

I

E

G

G

S

T

W

Q

G

G

R

I

L

G

V

V

A

V

R

L

A

A

D

E

S

T

R

R

E

Q

S

A

L

A

E

E

A

S

V

V

I

I

E

D

H

A

R

F

E

R

V

G

L

L

G

N

P

A

A

H

Y

I

Y

L

K

-

V

-

H

-

Y

-

V

V

Q

Q

E
|
E

Y
|
Y

V
x
I

R
x
K

K

E

P

A

L

Q

R

G

-

C

D
|
D

I

I

R

R

V

C

F

L

V

V

I

V

G

G

D

D

E

E

V

V

P

V

V

A

A

A

I

I

-

E

Y

R

R

R

V

A

N

K

E

E

R

G

H

D

W

F

K
x
R

T
x
S

N
|
N

T

L

A

H

L

R

G

G

A

G

K

A

A

A

E

S

P

V

A

A

P

S

V

I

T

T

P

P

E

Q

L

E

R

R

E

E

L

I

A

A

L

I

R

K

A

A

K

R

A

T

V

M

G

A

G

L

G

D

V

V

L

A

G

G

I

V

D

D

V

I

F

L

E

R

D

-

P

A

E

N

R

R

G

G

L

P

L

L

V

V

N
x
M

E
|
E

I

V

N

N

A

A

N

S

S

P

D

G

F

L

K

E

N

G

T

I

E

E

R

K

V

T

T

T

G

G

F

I

N

D

M

I

A

A

R

G

A

K

I

M

V

I

E

R

Y

W

binding adenosine-5'-diphosphate: V141 (= V128), K143 (= K130), E180 (= E171), Y181 (= Y172), I182 (≠ V173), K183 (≠ R174), D189 (= D179), R213 (≠ K202), S214 (≠ T203), N215 (= N204), M261 (≠ N251), E262 (= E252)
binding glutamic acid: R98 (≠ N86), R104 (≠ L92), Q107 (≠ I95), L108 (= L96), R111 (≠ K99)

7qyrD Crystal structure of rimk from pseudomonas aeruginosa pao1 (see paper)
30% identity, 98% coverage: 1:277/284 of query aligns to 1:285/293 of 7qyrD

query
sites
7qyrD

M

M

R

K

I

I

A

A

I

V

V

L

Y
x
S

D

R

H

N

P

P

R

R

V
x
L

-

Y

E
x
S

E

T

K

R

R

R

L

L

A

V

E

E

E

A

A

G

R

R

K

E

L

R

G

G

H

H

E

E

P

M

V

V

L

V

-

I

-

D

-

T

-

L

-

R

-

A

-

Y

F

M

N

N

I

I

D

A

S

S

L

H

L

K

F

P

R

Q

L

I

D

H

S

Y

L

R

E

G

R

Q

I

P

L

L

G

E

D

G

V

F

D

D

V

A

V

V

L

I

Q

P

R
|
R

-

I

-

G

A

A

V

S

S

V

Y

T

F

F

K

Y

A

G

L

C

E

A

S

V

T

L

R

R

I

Q

L

F

E

E

A

M

G

G

Y

V

T

F

V

P

I

L

N

N

N

E

S

S

L

V

V

A

Q

I

L

A

N

R

C

S

G

R

D

D

K

K

L

L

L

R

T

S

T

L

I

Q

L

L

A

S

K

R

H

K

G

G

V

I

P

G

T

L

P

P

R

V

A

T

Y

G

A

F

A

A

F

H

S

S

R

P

D

D

T

D

A

V

V

P

R

D

A

L

A

I

E

E

E

M

L

V

G

G

-

G

Y

A

P

P

V

L

V
|
V

V

I

K

K

P

L

V

L

I

E

G

G

S

T

W

Q

G

G

R

I

L

G

V

V

A

V

R

L

A

C

D

E

S

T

R

E

E

K

S

A

L

A

E

E

A

S

V

V

I

L

E

E

H

-

R

-

E

-

V

-

L

-

G

-

P

-

A

A

Y

F

Y

M

K

G

V

L

H

K

Y

H

-

N

-

I

-

M

V

V

Q

Q

E

E

Y
|
Y

V
x
I

R

K

K

E

P

A

L

G

-

G

R

A

D
|
D

I

I

R
|
R

V

C

F

F

V

V

I

V

G

G

D

D

E

K

V

V

P

I

V

A

A

S

I

M

Y

K

R

R

-

Q

V

A

N

A

E

P

R

G

H

E

W

F

K
x
R

T
x
S

N

N

T

L

A

H

L

R

G

G

A

G

K

S

A

A

E

S

P

L

A

I

P

K

V

I

T

T

P

P

E

E

L

E

R

R

E

M

L

T

A

A

L

I

R

R

A

A

K

R

A

V

V

M

G

G

G

L

G

N

V

V

L

A

G

G

I

V

D

D

V

I

F
x
L

E

R

D

S

P

-

E

N

R

H

G

G

L

P

L

L

V

V

N
x
M

E

E

I

V

N
|
N

A

S

N
x
S

S

P

D
x
G

F

L

K

E

N

G

T

I

E

E

R

S

V

T

T

T

G

G

F

K

N

D

M

I

A

A

R

G

A

I

I

I

V

I

E

Q

Y

Y

binding adenosine-5'-diphosphate: V139 (= V128), Y179 (= Y172), I180 (≠ V173), D187 (= D179), R211 (≠ K202), S212 (≠ T203), L250 (≠ F241), M259 (≠ N251)
binding : S7 (≠ Y7), L12 (≠ V12), S14 (≠ E13), R64 (= R56), R189 (= R181), N262 (= N254), S264 (≠ N256), G266 (≠ D258)

7lgmA Cyanophycin synthetase from a. Baylyi dsm587 with atp (see paper)
39% identity, 24% coverage: 87:154/284 of query aligns to 215:283/719 of 7lgmA

query
sites
7lgmA

C

A

G

G

D

D

K

K

L

N

L

V

T

C

T

N

I

Q

L

L

A

Q

K

D

H

L

G

G

V

L

P

P

T

V

P

P

R

K

A

Q

Y

R

A

V

F

Y

S

D

R

I

D

D

T

D

A

A

V

V

R

R

A

A

E

R

L

V

G

G

Y

F

P

P

V

V

V
|
V

V

L

K

K

P

P

V

L

I

D

G

G

S

N

W

H

G

G

R
|
R

L

G

V

V

A

S

-

V

R

N

A

L

D

T

S

T

R

D

E

E

S

A

L

V

E

E

A

A

V

A

I

F

E

D

binding adenosine-5'-triphosphate: V256 (= V128), R266 (= R138)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 297, 298, 302, 428

2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
28% identity, 65% coverage: 88:272/284 of query aligns to 114:308/444 of 2vr1A

query
sites
2vr1A

G

G

D

D

K
|
K

L

V

L

S

T

A

T

I

I

A

L

A

L

M

A

K

K

K

H

A

G

G

V

V

P

P

T

C

-

V

P

P

R

G

A

S

Y

D

A

G

A

P

F

L

S

G

R

D

D

D

T

M

-

D

A

K

V

N

R

R

A

A

-

I

A

A

E

K

E

R

L

I

G

G

Y

Y

P

P

V

V

V

I

K
|
K

P

A

V

S

I

G

G

G

S

G

W
x
R

G

G

R
x
M

L

R

V

V

A

V

R

R

A

G

D

D

S

A

R

E

E

L

S

A

L

Q

E

S

A

I

V

S

I

M

E

T

H

R

R

A

E

E

V

A

L

K

G

A

P

A

A

F

Y

S

Y

N

K
x
D

V

M

H

V

Y

Y

V

M

Q

E

E

K

Y
|
Y

V
x
L

R

E

K

N

P

P

L

-

R

R

D
x
H

I

V

R

E

V

I

F

Q

V

V

I

L

G

A

D

D

E

G

V

Q

P

G

V

N

A

A

I

I

Y

Y

-

L

-

A

-

E

-

R

-

D

-

C

R

S

V

M

N

Q

E

R

R
|
R

H

H

W

Q

K

K

T

V

N

-

T

-

A

-

L

-

G

-

A

V

K

E

A

E

E

A

P

P

A

A

P

P

-

G

V

I

T

T

P

P

E

E

L

L

R

R

E

R

-

Y

L

I

A

G

L

E

R

R

A

C

A

A

K

K

A

A

V

C

-

V

-

D

-

I

-

G

-

Y

-

R

G

G

G

A

G

G

V
x
T

L

F

G
x
E

I

F

D

-

V
x
L

F

F

E

E

D

N

P

G

E

E

R

F

G

Y

L

F

L

I

V
x
E

N

M

E
x
N

I

T

N
x
R

A

I

N

Q

S

V

D
x
E

F

H

K

P

N

V

T

T

E

E

R

M

V

I

T

T

G

G

F

V

N

D

M

L

A

I

R

K

active site: K116 (= K90), K159 (= K130), D194 (≠ K165), H207 (≠ D179), R233 (= R199), T272 (≠ V235), E274 (≠ G237), E286 (≠ V250), N288 (≠ E252), R290 (≠ N254), E294 (≠ D258)
binding phosphodifluoromethylphosphonic acid-adenylate ester: K159 (= K130), R165 (≠ W136), M167 (≠ R138), Y201 (= Y172), L202 (≠ V173), E274 (≠ G237), L276 (≠ V240), E286 (≠ V250), N288 (≠ E252)

Sites not aligning to the query:

active site: 336
binding phosphodifluoromethylphosphonic acid-adenylate ester: 435

7drpA Structure of atp-grasp ligase psnb complexed with phosphomimetic variant of minimal precursor, mg, and adp (see paper)
25% identity, 75% coverage: 71:284/284 of query aligns to 113:312/312 of 7drpA

query
sites
7drpA

E

E

A

E

A

A

G

G

Y

T

T

A

V

V

I

Y

N

N

N

S

S

P

L

R

V

A

Q

S

L

A

N

N

C

I

G

T

D

-

K

K

L

P

L

F

T

Q

T

L

I

A

L

L

A

R

K

D

H

A

G

G

V

L

P

P

T

V

P

P

R

R

A

S

Y

L

A

W

A

T

F

N

S

D

R

P

D

E

T

A

A

V

V

R

R

R

A

F

A

H

E

A

E

E

L

V

G

G

Y

-

P

D

V

C

V

I

V
x
Y

K
|
K

P

P

V

V

I

A

G

G

S

-

W

-

G
|
G

R
x
A

L

R

V
x
T

A
x
R

R

K

A

L

D

E

S

A

R

K

E

D

S

-

L

L

E

E

A

A

V

D

I
x
R

E

I

H

E

R
|
R

E
x
L

V

S

L

A

G

A

P

P

A
x
V

Y

C

Y

F

K
x
Q

V
x
E

H

L

Y
x
L

V

T

Q

G

E

D

Y

-

V

-

R

-

K

-

P

-

L

-

R

-

D
|
D

I

V

R
|
R

V

V

F

Y

V

V

I

I

G

D

D

D

E

Q

V

V

P

I

V

C

A

A

I

L

Y

R

R

I

V

V

-

T

N

D

E

E

R

I

H

D

W

F

K

R

T

Q

N

A

T

E

A

E

L

-

G

-

A

-

K

R

A

I

E

E

P

A

A

I

P

E

V

I

T

S

P

D

E

E

L

V

R

K

E

D

L

Q

A

C

L

V

R

R

A

A

K

K

A

L

V

V

G

G

G

L

G

R

V

Y

L

T

G

G

I

M

D
|
D

V

I

F
x
K

E

A

D

G

P

A

E

D

R

G

G

N

L

Y

L

R

V

V

N
x
L

E
|
E

I

L

N
|
N

A

A

N
x
S

S
x
A

D
x
M

F

F

K

R

N

G

T

F

E

E

R

G

V

R

T

A

G

N

F

V

N

D

M

I

A

C

R

G

A

P

I

L

V

C

E

D

Y

A

A

L

V

I

S

A

V

Q

A

T

K

K

R

R

binding adenosine-5'-diphosphate: K170 (= K130), T178 (≠ V140), Q202 (≠ K165), E203 (≠ V166), L205 (≠ Y168), D209 (= D179), K269 (≠ F241), L279 (≠ N251), E280 (= E252)
binding magnesium ion: D267 (= D239), E280 (= E252), E280 (= E252), N282 (= N254)
binding : Y169 (≠ V129), G175 (= G137), A176 (≠ R138), R179 (≠ A141), R190 (≠ I153), R193 (= R156), L194 (≠ E157), V199 (≠ A162), R211 (= R181), N282 (= N254), S284 (≠ N256), A285 (≠ S257), M286 (≠ D258)

Sites not aligning to the query:

binding : 70, 77, 79

Query Sequence

>WP_014026286.1 NCBI__GCF_000223395.1:WP_014026286.1
MRIAIVYDHPRVEEKRLAEEARKLGHEPVLFNIDSLLFRLDSLERILGDVDVVLQRAVSY
FKALESTRILEAAGYTVINNSLVQLNCGDKLLTTILLAKHGVPTPRAYAAFSRDTAVRAA
EELGYPVVVKPVIGSWGRLVARADSRESLEAVIEHREVLGPAYYKVHYVQEYVRKPLRDI
RVFVIGDEVPVAIYRVNERHWKTNTALGAKAEPAPVTPELRELALRAAKAVGGGVLGIDV
FEDPERGLLVNEINANSDFKNTERVTGFNMARAIVEYAVSVAKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory