SitesBLAST
Comparing WP_014026339.1 NCBI__GCF_000223395.1:WP_014026339.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
39% identity, 89% coverage: 1:260/291 of query aligns to 1:252/267 of 2hk9B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ I66), G130 (= G127), G133 (= G130), A134 (= A131), N153 (= N151), R154 (= R152), T155 (= T153), K158 (≠ R156), T188 (= T193), S189 (≠ P194), V190 (≠ L195), I214 (≠ A220), M238 (= M246), L239 (= L247)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S19), S21 (= S21), N64 (= N63), T66 (= T65), K70 (= K69), N91 (= N90), D106 (= D105), Y216 (= Y222), L239 (= L247), Q242 (= Q250)
O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
39% identity, 89% coverage: 1:260/291 of query aligns to 1:252/269 of O67049
- SLS 19:21 (= SLS 19:21) binding shikimate
- D82 (≠ S81) binding NADP(+)
- N91 (= N90) binding shikimate
- D106 (= D105) binding shikimate
- GAGGA 130:134 (= GAGGA 127:131) binding NADP(+)
- I214 (≠ A220) binding NADP(+)
- Y216 (= Y222) binding shikimate
- G235 (= G243) binding NADP(+)
- Q242 (= Q250) binding shikimate
2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
39% identity, 89% coverage: 1:260/291 of query aligns to 1:252/269 of 2hk9A
- binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ I66), G132 (= G129), G133 (= G130), A134 (= A131), N153 (= N151), R154 (= R152), T155 (= T153), T188 (= T193), S189 (≠ P194), V190 (≠ L195)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S19), S21 (= S21), N64 (= N63), K70 (= K69), N91 (= N90), D106 (= D105), Y216 (= Y222), L239 (= L247), Q242 (= Q250)
Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
35% identity, 93% coverage: 1:271/291 of query aligns to 1:275/282 of Q58484
1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
35% identity, 93% coverage: 1:271/291 of query aligns to 6:280/287 of 1nvtB
- active site: K75 (= K69), D111 (= D105)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (= I66), G135 (≠ L126), G137 (≠ A128), G138 (= G129), A139 (≠ G130), N157 (= N151), R158 (= R152), T159 (= T153), K162 (≠ R156), A200 (= A192), T201 (= T193), P202 (= P194), I203 (≠ L195), M205 (= M197), L229 (≠ A220), Y231 (= Y222), M255 (= M246), L256 (= L247)
- binding zinc ion: E22 (≠ A17), H23 (≠ Q18)
1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
35% identity, 93% coverage: 1:271/291 of query aligns to 6:280/287 of 1nvtA
- active site: K75 (= K69), D111 (= D105)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (≠ L126), A139 (≠ G130), N157 (= N151), R158 (= R152), T159 (= T153), K162 (≠ R156), A200 (= A192), T201 (= T193), P202 (= P194), I203 (≠ L195), M205 (= M197), L229 (≠ A220), Y231 (= Y222), G252 (= G243), M255 (= M246), L256 (= L247)
3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
32% identity, 88% coverage: 2:258/291 of query aligns to 9:276/291 of 3tozA
- binding nicotinamide-adenine-dinucleotide: G137 (= G127), A138 (= A128), G139 (= G129), G140 (= G130), A141 (= A131), N161 (= N151), R162 (= R152), D164 (≠ R154), F166 (≠ R156), T210 (= T193), G211 (≠ P194), V212 (≠ L195), M214 (= M197), F217 (≠ V200), V238 (≠ A220), Y240 (= Y222), G261 (= G243), M264 (= M246), M265 (≠ L247)
Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
32% identity, 88% coverage: 2:258/291 of query aligns to 9:276/291 of Q8Y9N5
3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
32% identity, 88% coverage: 2:258/291 of query aligns to 6:273/288 of 3tnlA
- binding nicotinamide-adenine-dinucleotide: M71 (≠ I66), G134 (= G127), A135 (= A128), G136 (= G129), G137 (= G130), A138 (= A131), N158 (= N151), R159 (= R152), D161 (≠ R154), F163 (≠ R156), T207 (= T193), V209 (≠ L195), M211 (= M197), F214 (≠ V200), V235 (≠ A220), Y237 (= Y222), M261 (= M246), M262 (≠ L247)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S19), S25 (= S21), N68 (= N63), S70 (≠ T65), K74 (= K69), N95 (= N90), D110 (= D105), Q265 (= Q250)
Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
29% identity, 93% coverage: 9:280/291 of query aligns to 3:266/269 of Q5HNV1
- SLS 13:15 (= SLS 19:21) binding shikimate
- T60 (= T65) binding shikimate
- N85 (= N90) binding shikimate
- D100 (= D105) binding shikimate
- Y211 (= Y222) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
- Q239 (= Q250) binding shikimate
2cy0A Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP (see paper)
39% identity, 91% coverage: 9:274/291 of query aligns to 4:260/262 of 2cy0A
- active site: K64 (= K69), D100 (= D105)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G123 (= G127), G126 (= G130), A127 (= A131), N146 (= N151), R147 (= R152), T148 (= T153), R151 (= R156), T179 (= T193), R180 (≠ P194), V181 (≠ L195), L205 (≠ A220), L232 (= L247)
2ev9B Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP(h) and shikimate (see paper)
39% identity, 91% coverage: 9:274/291 of query aligns to 4:260/263 of 2ev9B
- active site: K64 (= K69), D100 (= D105)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S19), S16 (= S21), N58 (= N63), T60 (= T65), K64 (= K69), N85 (= N90), D100 (= D105), Q235 (= Q250)
Q5SJF8 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
39% identity, 91% coverage: 9:274/291 of query aligns to 4:260/263 of Q5SJF8
- SLS 14:16 (= SLS 19:21) binding shikimate
- T60 (= T65) binding shikimate
- K64 (= K69) active site, Proton acceptor
- N85 (= N90) binding shikimate
- D100 (= D105) binding shikimate
- GAGGA 123:127 (= GAGGA 127:131) binding NADP(+)
- NRTPQR 146:151 (≠ NRTRSR 151:156) binding NADP(+)
- L205 (≠ A220) binding NADP(+)
- Y207 (= Y222) binding shikimate
- G228 (= G243) binding NADP(+)
- Q235 (= Q250) binding shikimate
2o7sA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
36% identity, 91% coverage: 3:268/291 of query aligns to 230:484/500 of 2o7sA
- binding 3-dehydroshikimate: I239 (≠ L11), S247 (= S19), S249 (= S21), T292 (= T65), K296 (= K69), N317 (= N90), D334 (= D105), Y438 (= Y222), Q466 (= Q250), Q470 (≠ A254)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I293 (= I66), P294 (= P67), K296 (= K69), D334 (= D105), G354 (= G129), G355 (= G130), A356 (= A131), N374 (= N151), R375 (= R152), T376 (= T153), R379 (= R156), T409 (= T193), S410 (≠ P194), M411 (≠ L195), A436 (= A220), M462 (= M246), F463 (≠ L247)
Sites not aligning to the query:
2o7qA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
36% identity, 91% coverage: 3:268/291 of query aligns to 230:485/501 of 2o7qA
Sites not aligning to the query:
3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
28% identity, 93% coverage: 9:280/291 of query aligns to 3:257/258 of 3dooA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S19), S15 (= S21), N58 (= N63), T60 (= T65), K64 (= K69), N85 (= N90), D100 (= D105), F227 (≠ L247), Q230 (= Q250)
2gptA Crystal structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate (see paper)
35% identity, 91% coverage: 3:268/291 of query aligns to 230:482/498 of 2gptA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: I239 (≠ L11), S247 (= S19), S249 (= S21), T292 (= T65), K296 (= K69), N317 (= N90), D334 (= D105), Y436 (= Y222), Q464 (= Q250), Q468 (≠ A254)
Sites not aligning to the query:
6bmqA Crystal structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase (t381g mutant) in complex with tartrate and shikimate (see paper)
35% identity, 91% coverage: 3:268/291 of query aligns to 230:482/498 of 6bmqA
- binding (1S,3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid: S247 (= S19), S249 (= S21), C291 (≠ V64), K296 (= K69), N317 (= N90), D334 (= D105), Y436 (= Y222), Q464 (= Q250)
Sites not aligning to the query:
Q9SQT8 Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic; DHQ-SDH protein; DHQase-SORase; Protein EMBRYO DEFECTIVE 3004; EC 4.2.1.10; EC 1.1.1.25 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 91% coverage: 3:268/291 of query aligns to 319:596/603 of Q9SQT8
- S336 (= S19) binding shikimate; mutation to A: 13-fold decrease in substrate affinity but almost no change in activity.
- S338 (= S21) binding shikimate; mutation to A: 10-fold decrease in activity, and 9-fold decrease in substrate affinity.
- T381 (= T65) binding shikimate
- K385 (= K69) binding shikimate; mutation to A: Strongly reduced shikimate dehydrogenase activity, but minor change in substrate affinity.; mutation to N: Strongly reduced shikimate dehydrogenase activity, but no change in substrate affinity.
- N406 (= N90) binding shikimate
- D423 (= D105) binding shikimate; mutation to A: Loss of shikimate dehydrogenase activity.; mutation to N: Reduced shikimate dehydrogenase activity, but no change in substrate affinity.
- A461 (= A128) binding NADP(+)
- G463 (= G130) binding NADP(+)
- A464 (= A131) binding NADP(+)
- N483 (= N151) binding NADP(+)
- T485 (= T153) binding NADP(+)
- R488 (= R156) binding NADP(+)
- M525 (= M197) binding NADP(+)
- A548 (= A220) binding NADP(+)
- Y550 (= Y222) binding shikimate; mutation Y->F,A: 100-fold decrease in activity, and 2-fold decrease in substrate affinity.
- G571 (= G243) binding NADP(+)
- Q578 (= Q250) binding shikimate
- Q582 (≠ A254) binding shikimate
Sites not aligning to the query:
- 124 binding 3-dehydroshikimate
- 126 binding 3-dehydroshikimate
- 155 binding 3-dehydroshikimate
- 241 binding 3-dehydroshikimate
- 279 binding 3-dehydroshikimate
- 300 binding 3-dehydroshikimate
- 304 binding 3-dehydroshikimate
1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
29% identity, 90% coverage: 7:269/291 of query aligns to 8:282/288 of 1npdB
- binding nicotinamide-adenine-dinucleotide: A132 (= A128), G133 (= G129), G134 (= G130), A135 (= A131), N155 (= N151), R156 (= R152), D158 (≠ R154), F160 (≠ R156), T204 (= T193), K205 (≠ P194), V206 (≠ L195), M208 (= M197), C232 (≠ A220), M258 (= M246), L259 (= L247)
Query Sequence
>WP_014026339.1 NCBI__GCF_000223395.1:WP_014026339.1
MLVGPKRLYGLIGHPVAQSLSPVIHESVYRKVGVEAVYLLWDVEGREVHFVVEGLRNLAN
GFNVTIPHKERVRAHCDSESSEVSILGAVNTVKVEGAKLHCFNTDVYGVERCLRGLASDG
FTMLVLGAGGAAKAAVLAAQHLGASTVIISNRTRSRAVQLAGIAEALGLRARVVAWPPPR
EAIEEADVLFNATPLGMEGVGGAPPVDTDAISPKHVVFDAIYKPLETPLIRAARERNART
VDGLCMLVWQALEADRIWLGIDPSEKLYQAARKAALEALQRAVSRRARSTS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory