SitesBLAST
Comparing WP_014027034.1 NCBI__GCF_000223395.1:WP_014027034.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4uqvG Methanococcus jannaschii serine hydroxymethyl-transferase in complex with plp (see paper)
48% identity, 95% coverage: 9:428/441 of query aligns to 4:418/429 of 4uqvG
- active site: Y50 (= Y58), E52 (= E60), D196 (= D209), S223 (= S236), K226 (= K239), Q232 (= Q245)
- binding pyridoxal-5'-phosphate: G93 (= G101), V94 (≠ T102), H121 (= H134), A198 (= A211), H199 (= H212), S223 (= S236), H225 (= H238), K226 (= K239)
4bhdB Methanococcus jannaschii serine hydroxymethyl-transferase, apo form (see paper)
44% identity, 96% coverage: 7:428/441 of query aligns to 1:384/395 of 4bhdB
6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
33% identity, 88% coverage: 29:415/441 of query aligns to 25:402/418 of 6ymfA
- active site: Y54 (= Y58), E56 (= E60), D200 (= D209), T226 (≠ S236), K229 (= K239), R235 (≠ Q245)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S38), S96 (= S100), G97 (= G101), A98 (≠ T102), H125 (= H134), S175 (= S184), D200 (= D209), A202 (= A211), H203 (= H212), T226 (≠ S236), K229 (= K239), R361 (= R373)
6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
33% identity, 88% coverage: 29:415/441 of query aligns to 25:402/420 of 6ymdA
- active site: Y54 (= Y58), E56 (= E60), D200 (= D209), T226 (≠ S236), K229 (= K239), R235 (≠ Q245)
- binding malonate ion: S34 (= S38), Y54 (= Y58), E56 (= E60), Y64 (= Y68), H125 (= H134), H203 (= H212), K229 (= K239), R361 (= R373)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y54 (= Y58), S96 (= S100), G97 (= G101), A98 (≠ T102), H125 (= H134), Y174 (≠ G183), S175 (= S184), D200 (= D209), A202 (= A211), T226 (≠ S236), K229 (= K239), G261 (≠ N271)
4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
33% identity, 92% coverage: 17:420/441 of query aligns to 16:412/416 of 4n0wA
- active site: Y57 (= Y58), E59 (= E60), D202 (= D209), T228 (≠ S236), K231 (= K239), R237 (≠ Q245)
- binding pyridoxal-5'-phosphate: S99 (= S100), G100 (= G101), S101 (≠ T102), H128 (= H134), D202 (= D209), A204 (= A211), H205 (= H212), K231 (= K239)
4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
33% identity, 92% coverage: 17:420/441 of query aligns to 9:405/409 of 4otlA
- active site: Y50 (= Y58), E52 (= E60), D195 (= D209), T221 (≠ S236), K224 (= K239), R230 (≠ Q245)
- binding glycine: S30 (= S38), Y50 (= Y58), Y60 (= Y68), H121 (= H134), K224 (= K239), R355 (= R373)
- binding pyridoxal-5'-phosphate: S92 (= S100), G93 (= G101), S94 (≠ T102), H121 (= H134), S170 (= S184), D195 (= D209), A197 (= A211), H198 (= H212), K224 (= K239)
4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
33% identity, 92% coverage: 17:420/441 of query aligns to 14:410/414 of 4ot8A
- active site: Y55 (= Y58), E57 (= E60), D200 (= D209), T226 (≠ S236), K229 (= K239), R235 (≠ Q245)
- binding pyridoxal-5'-phosphate: S97 (= S100), G98 (= G101), S99 (≠ T102), H126 (= H134), D200 (= D209), A202 (= A211), H203 (= H212), K229 (= K239)
- binding serine: S35 (= S38), E57 (= E60), Y65 (= Y68), H126 (= H134), H203 (= H212), R360 (= R373)
3pgyB Serine hydroxymethyltransferase from staphylococcus aureus, s95p mutant.
32% identity, 87% coverage: 27:411/441 of query aligns to 20:389/404 of 3pgyB
Sites not aligning to the query:
7x5nA Crystal structure of e. Faecium shmt in complex with (+)-shin-1 and plp-ser (see paper)
33% identity, 90% coverage: 24:420/441 of query aligns to 14:407/409 of 7x5nA
- binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E60), Y58 (= Y67), Y59 (= Y68), L115 (≠ V129), G119 (≠ A133), H120 (= H134), L121 (≠ V135), K340 (= K355), N341 (= N356), S342 (≠ L357), P350 (≠ A365), F351 (≠ I366), R357 (= R373)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S29 (= S38), Y49 (= Y58), E51 (= E60), Y59 (= Y68), S91 (= S100), G92 (= G101), S93 (≠ T102), H120 (= H134), S170 (= S184), D195 (= D209), A197 (= A211), H198 (= H212), K224 (= K239), R357 (= R373)
7v3dA Complex structure of serine hydroxymethyltransferase from enterococcus faecium and its inhibitor (see paper)
33% identity, 90% coverage: 24:420/441 of query aligns to 14:407/409 of 7v3dA
- binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E60), Y58 (= Y67), L115 (≠ V129), G119 (≠ A133), H120 (= H134), L121 (≠ V135), K340 (= K355), S342 (≠ L357), P350 (≠ A365), F351 (≠ I366), R357 (= R373)
- binding pyridoxal-5'-phosphate: Y49 (= Y58), S91 (= S100), G92 (= G101), S93 (≠ T102), H120 (= H134), S170 (= S184), D195 (= D209), A197 (= A211), K224 (= K239), G255 (≠ S270)
7x5oB Crystal structure of e. Faecium shmt in complex with me-thf and plp- gly (see paper)
33% identity, 90% coverage: 24:420/441 of query aligns to 15:408/412 of 7x5oB
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S30 (= S38), Y50 (= Y58), Y60 (= Y68), S92 (= S100), G93 (= G101), S94 (≠ T102), H121 (= H134), S171 (= S184), D196 (= D209), A198 (= A211), H199 (= H212), K225 (= K239), R358 (= R373)
- binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E52 (= E60), Y59 (= Y67), L116 (≠ V129), G119 (= G132), G120 (≠ A133), H121 (= H134), S171 (= S184), P252 (= P266), N342 (= N356), P351 (≠ A365)
Q5SI56 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
31% identity, 90% coverage: 18:413/441 of query aligns to 11:397/407 of Q5SI56
- Y51 (= Y58) binding
- GS 94:95 (≠ GT 101:102) binding
- S172 (= S184) binding
- H200 (= H212) binding
- H225 (= H238) binding
- K226 (= K239) modified: N6-(pyridoxal phosphate)lysine
- G258 (≠ N271) binding
8suiB Joint x-ray/neutron structure of thermus thermophilus serine hydroxymethyltransferase (tthshmt) in internal aldimine state with l- ser bound in a pre-michalis complex (see paper)
31% identity, 90% coverage: 18:413/441 of query aligns to 6:392/402 of 8suiB
8ssyA Room-temperature x-ray structure of thermus thermophilus serine hydroxymethyltransferase (shmt) bound with d-ser in a pseudo- michaelis complex (see paper)
31% identity, 90% coverage: 18:413/441 of query aligns to 6:392/402 of 8ssyA
2dkjA Crystal structure of t.Th.Hb8 serine hydroxymethyltransferase
31% identity, 90% coverage: 18:413/441 of query aligns to 6:392/402 of 2dkjA
- active site: Y46 (= Y58), E48 (= E60), D192 (= D209), T218 (≠ S236), K221 (= K239), R227 (≠ Q245)
- binding pyridoxal-5'-phosphate: S88 (= S100), G89 (= G101), S90 (≠ T102), H117 (= H134), S167 (= S184), D192 (= D209), A194 (= A211), H220 (= H238), K221 (= K239)
4wxgA Crystal structure of l-serine hydroxymethyltransferase in complex with a mixture of l-threonine and glycine (see paper)
33% identity, 92% coverage: 17:423/441 of query aligns to 8:402/410 of 4wxgA
- active site: T43 (≠ N52), L45 (≠ M54), G189 (= G203), A215 (= A230), T218 (≠ M233), R230 (≠ Q245)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-threonine: S29 (= S38), Y49 (= Y58), E51 (= E60), Y59 (= Y68), S91 (= S100), G92 (= G101), S93 (≠ T102), H120 (= H134), S170 (= S184), D195 (= D209), A197 (= A211), H198 (= H212), T221 (≠ S236), K224 (= K239), G255 (≠ S270), R357 (= R373)
4wxfA Crystal structure of l-serine hydroxymethyltransferase in complex with glycine (see paper)
33% identity, 92% coverage: 17:423/441 of query aligns to 8:402/410 of 4wxfA
- active site: T43 (≠ N52), L45 (≠ M54), G189 (= G203), A215 (= A230), T218 (≠ M233), R230 (≠ Q245)
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S29 (= S38), Y49 (= Y58), Y59 (= Y68), S91 (= S100), G92 (= G101), S93 (≠ T102), H120 (= H134), S170 (= S184), D195 (= D209), A197 (= A211), H198 (= H212), H223 (= H238), K224 (= K239), G255 (≠ S270), R357 (= R373)
1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
32% identity, 88% coverage: 11:400/441 of query aligns to 4:384/405 of 1kl2A
- active site: Y51 (= Y58), E53 (= E60), D197 (= D209), T223 (≠ S236), K226 (= K239), R232 (≠ Q245)
- binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E60), Y60 (= Y67), G121 (≠ A133), H122 (= H134), S172 (= S184), F251 (= F265), N341 (= N356)
- binding glycine: S31 (= S38), Y51 (= Y58), Y61 (= Y68), H200 (= H212), R357 (= R373)
- binding pyridoxal-5'-phosphate: S93 (= S100), G94 (= G101), A95 (≠ T102), H122 (= H134), S172 (= S184), D197 (= D209), A199 (= A211), H200 (= H212), T223 (≠ S236), H225 (= H238), K226 (= K239)
1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
32% identity, 88% coverage: 11:400/441 of query aligns to 4:384/405 of 1kl1A
- active site: Y51 (= Y58), E53 (= E60), D197 (= D209), T223 (≠ S236), K226 (= K239), R232 (≠ Q245)
- binding glycine: S31 (= S38), H122 (= H134), R357 (= R373)
- binding pyridoxal-5'-phosphate: S93 (= S100), G94 (= G101), A95 (≠ T102), H122 (= H134), A171 (≠ G183), S172 (= S184), D197 (= D209), A199 (= A211), H200 (= H212), T223 (≠ S236), H225 (= H238), K226 (= K239)
1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
32% identity, 88% coverage: 11:400/441 of query aligns to 4:384/405 of 1kkpA
- active site: Y51 (= Y58), E53 (= E60), D197 (= D209), T223 (≠ S236), K226 (= K239), R232 (≠ Q245)
- binding pyridoxal-5'-phosphate: S93 (= S100), G94 (= G101), A95 (≠ T102), H122 (= H134), S172 (= S184), D197 (= D209), A199 (= A211), H200 (= H212), K226 (= K239)
- binding serine: S31 (= S38), H122 (= H134), R357 (= R373)
Query Sequence
>WP_014027034.1 NCBI__GCF_000223395.1:WP_014027034.1
MVSWDEILARLPSEVREVIETTRAHNRWRRETINLIASENVMSPLAEAAYLNDMMHRYAE
GKPRKRYYQGLRYVDVVEELVMKYMGELLGGAFIEPRPVSGTIANATVFRALASCPSSEG
RPKALIAPVQAGAHVSHTKFGTLGALCIEHIELPYDPDNLNVDVDKAVKMIEDVKPVFVV
LGGSLYLFPHPVREIAEAAHSVGAKLVYDAAHVLGLIVGKRWRNPLDHGADIMTASTHKT
FPGPQGGIIATRQEDLYKAVSRIVFPYFVSNHHLHRLPALAITALEMKYYGEAYADQVVR
NAKALAEALAAEGFKVLGEHLGYTRSHQVAVDVREYGGGAKAAQLLEEANIIVNKNLLPY
DPPDAIKNPSGLRLGVQEMTRWGMKEDDMREIARFMRRVVIDGEDPAKVRKEVVEFRRNF
QKVHYAFDVDPTENGKLLLLY
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory