SitesBLAST
Comparing WP_014148057.1 NCBI__GCF_000968535.2:WP_014148057.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4itbA Structure of bacterial enzyme in complex with cofactor and substrate (see paper)
52% identity, 100% coverage: 2:454/454 of query aligns to 1:453/453 of 4itbA
- active site: N130 (= N131), K153 (= K154), E227 (= E228), C261 (= C262), E358 (= E359), E435 (= E436)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V126 (= V127), M127 (= M128), P128 (= P129), W129 (= W130), N130 (= N131), K153 (= K154), A155 (= A156), S156 (= S157), A186 (≠ S187), V189 (= V190), G205 (= G206), S206 (= S207), A209 (= A210), S212 (= S213), L228 (= L229), C261 (= C262), E358 (= E359), F360 (= F361)
- binding 4-oxobutanoic acid: E227 (= E228), C261 (= C262), S418 (= S419)
3vz3A Structural insights into substrate and cofactor selection by sp2771 (see paper)
52% identity, 100% coverage: 2:454/454 of query aligns to 1:453/453 of 3vz3A
- active site: N130 (= N131), K153 (= K154), E227 (= E228), A261 (≠ C262), E358 (= E359), E435 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V126 (= V127), M127 (= M128), W129 (= W130), N130 (= N131), Q135 (= Q136), R138 (= R139), K153 (= K154), A155 (= A156), S156 (= S157), A186 (≠ S187), V189 (= V190), T204 (= T205), G205 (= G206), S206 (= S207), A209 (= A210), E227 (= E228), L228 (= L229), G229 (= G230), A261 (≠ C262), F360 (= F361)
- binding 4-oxobutanoic acid: F131 (= F132), W134 (= W135), S260 (= S261), A261 (≠ C262), I262 (= I263), S418 (= S419)
3efvA Crystal structure of a putative succinate-semialdehyde dehydrogenase from salmonella typhimurium lt2 with bound NAD (see paper)
48% identity, 99% coverage: 5:452/454 of query aligns to 8:455/459 of 3efvA
- active site: N134 (= N131), E231 (= E228), C265 (= C262), E439 (= E436)
- binding nicotinamide-adenine-dinucleotide: I130 (≠ V127), M131 (= M128), P132 (= P129), W133 (= W130), N134 (= N131), Q139 (= Q136), R142 (= R139), K157 (= K154), A159 (= A156), N190 (≠ S187), V193 (= V190), T208 (= T205), G209 (= G206), S210 (= S207), A213 (= A210), E231 (= E228), L232 (= L229), G233 (= G230), C265 (= C262), E362 (= E359), F364 (= F361), F428 (= F425)
4ywuA Structural insight into the substrate inhibition mechanism of NADP+- dependent succinic semialdehyde dehydrogenase from streptococcus pyogenes (see paper)
39% identity, 100% coverage: 2:453/454 of query aligns to 1:453/455 of 4ywuA
- active site: N131 (= N131), K154 (= K154), E228 (= E228), C262 (= C262), E359 (= E359), E436 (= E436)
- binding 4-oxobutanoic acid: N131 (= N131), Q136 (= Q136), R139 (= R139), E228 (= E228), V261 (≠ S261), C262 (= C262), F425 (= F425)
4ohtA Crystal structure of succinic semialdehyde dehydrogenase from streptococcus pyogenes in complex with NADP+ as the cofactor (see paper)
39% identity, 100% coverage: 2:453/454 of query aligns to 1:453/455 of 4ohtA
- active site: N131 (= N131), K154 (= K154), E228 (= E228), C262 (= C262), E359 (= E359), E436 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V127 (= V127), E128 (≠ M128), P129 (= P129), W130 (= W130), K154 (= K154), H155 (= H155), A156 (= A156), S157 (= S157), Y187 (≠ S187), S207 (= S207), I214 (≠ V214)
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
35% identity, 100% coverage: 2:453/454 of query aligns to 27:480/482 of P25526
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
34% identity, 100% coverage: 2:453/454 of query aligns to 26:479/481 of 3jz4A
- active site: N156 (= N131), K179 (= K154), E254 (= E228), C288 (= C262), E385 (= E359), E462 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P129), W155 (= W130), K179 (= K154), A181 (= A156), S182 (= S157), A212 (≠ V188), G216 (= G192), G232 (= G206), S233 (= S207), I236 (≠ A210), C288 (= C262), K338 (≠ E312), E385 (= E359), F387 (= F361)
4o6rA Crystal structure of a putative aldehyde dehydrogenase from burkholderia cenocepacia
32% identity, 99% coverage: 6:454/454 of query aligns to 24:478/489 of 4o6rA
- active site: N150 (= N131), K173 (= K154), E248 (= E228), C282 (= C262), E383 (= E359), E460 (= E436)
- binding adenosine monophosphate: I146 (≠ V127), V147 (≠ M128), K173 (= K154), G206 (= G186), G210 (≠ V190), Q211 (≠ R191), F224 (≠ L204), G226 (= G206), S227 (= S207), T230 (≠ A210), R233 (≠ S213)
4go4A Crystal structure of pnpe in complex with nicotinamide adenine dinucleotide
33% identity, 99% coverage: 6:454/454 of query aligns to 23:476/487 of 4go4A
- active site: N149 (= N131), K172 (= K154), E247 (= E228), C281 (= C262), E381 (= E359), E458 (= E436)
- binding nicotinamide-adenine-dinucleotide: I145 (≠ V127), V146 (≠ M128), W148 (= W130), N149 (= N131), F154 (≠ Q136), K172 (= K154), G205 (= G186), G209 (≠ V190), Q210 (≠ R191), F223 (≠ L204), T224 (= T205), G225 (= G206), S226 (= S207), T229 (≠ A210), E247 (= E228), G249 (= G230), C281 (= C262), E381 (= E359), F383 (= F361)
8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
33% identity, 98% coverage: 7:453/454 of query aligns to 49:497/505 of 8of1A
- binding nicotinamide-adenine-dinucleotide: I170 (≠ V127), A171 (≠ M128), P172 (= P129), W173 (= W130), K197 (= K154), A230 (≠ V188), F248 (≠ L204), G250 (= G206), S251 (= S207), V254 (≠ A210), M257 (≠ S213), L273 (= L229), C306 (= C262), K356 (≠ E312), E403 (= E359), F405 (= F361)
P25553 Lactaldehyde dehydrogenase; Aldehyde dehydrogenase A; Glycolaldehyde dehydrogenase; EC 1.2.1.22; EC 1.2.1.21 from Escherichia coli (strain K12) (see 5 papers)
33% identity, 99% coverage: 6:454/454 of query aligns to 27:478/479 of P25553
- L150 (≠ M128) binding NAD(+)
- R161 (= R139) binding (S)-lactate
- KPSE 176:179 (≠ KHAS 154:157) binding NAD(+)
- F180 (≠ N158) mutation to T: Can bind and use NADP(+) as coenzyme. 16-fold increase in catalytic efficiency with NAD(+) as coenzyme.
- Q214 (vs. gap) binding NAD(+)
- S230 (= S207) binding NAD(+)
- E251 (= E228) binding (S)-lactate
- N286 (≠ I263) binding (S)-lactate; mutation to E: 4-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to H: 15-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to T: 6-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.
- R336 (≠ E312) binding NAD(+)
- E443 (≠ S419) binding (S)-lactate
- H449 (≠ F425) binding (S)-lactate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2impA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the ternary complex with lactate (occupancy 0.5) and nadh. Crystals soaked with (l)-lactate. (see paper)
33% identity, 99% coverage: 6:454/454 of query aligns to 25:476/477 of 2impA
- active site: N151 (= N131), K174 (= K154), E249 (= E228), C283 (= C262), E381 (= E359), A458 (≠ E436)
- binding 1,4-dihydronicotinamide adenine dinucleotide: I147 (≠ V127), L148 (≠ M128), P149 (= P129), W150 (= W130), K174 (= K154), E177 (≠ S157), F178 (≠ N158), G207 (≠ V188), G211 (= G192), Q212 (vs. gap), S228 (= S207), A231 (= A210), K234 (≠ S213), R334 (≠ E312)
2iluA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the binary complex with NADPH (see paper)
33% identity, 99% coverage: 6:454/454 of query aligns to 25:476/477 of 2iluA
- active site: N151 (= N131), K174 (= K154), E249 (= E228), C283 (= C262), E381 (= E359), A458 (≠ E436)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I147 (≠ V127), L148 (≠ M128), P149 (= P129), W150 (= W130), K174 (= K154), S176 (≠ A156), E177 (≠ S157), R206 (≠ S187), G207 (≠ V188), G211 (= G192), Q212 (vs. gap), S228 (= S207), A231 (= A210), K234 (≠ S213), I235 (≠ V214), N328 (≠ R306), R334 (≠ E312), F383 (= F361)
8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
33% identity, 98% coverage: 7:451/454 of query aligns to 31:477/482 of 8c54A
- binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (≠ V127), T153 (≠ M128), P154 (= P129), K179 (= K154), A212 (≠ V188), K213 (≠ Q189), F230 (≠ L204), T231 (= T205), G232 (= G206), S233 (= S207), V236 (≠ A210), W239 (≠ S213), G256 (= G230)
2opxA Crystal structure of lactaldehyde dehydrogenase from escherichia coli
32% identity, 99% coverage: 6:454/454 of query aligns to 25:476/477 of 2opxA
- active site: N151 (= N131), K174 (= K154), E249 (= E228), C283 (= C262), E381 (= E359), A458 (≠ E436)
- binding (3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid: F105 (≠ K86), F152 (= F132), N284 (≠ I263), F312 (≠ T291), G313 (= G292), R318 (≠ V297), D320 (= D298), I321 (≠ T299), A322 (≠ T300), Y362 (= Y340), F440 (≠ K418), F440 (≠ K418), E441 (≠ S419)
6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
30% identity, 99% coverage: 6:454/454 of query aligns to 22:476/477 of 6j76A
- active site: N148 (= N131), E246 (= E228), C280 (= C262), E458 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (≠ V127), T145 (≠ M128), A146 (≠ P129), W147 (= W130), N148 (= N131), K171 (= K154), T173 (≠ A156), S174 (= S157), G204 (≠ S187), G208 (≠ R191), T223 (= T205), G224 (= G206), S225 (= S207), A228 (= A210), S231 (= S213), I232 (≠ V214), E246 (= E228), L247 (= L229), C280 (= C262), E381 (= E359), F383 (= F361), H447 (≠ F425)
P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
35% identity, 91% coverage: 39:451/454 of query aligns to 112:530/535 of P51649
- G176 (≠ E103) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
- H180 (≠ E108) to Y: 83% of activity; dbSNP:rs2760118
- P182 (≠ D110) to L: 48% of activity; dbSNP:rs3765310
- R213 (= R139) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- C223 (≠ N149) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
- KPAE 228:231 (≠ KHAS 154:157) binding NAD(+)
- T233 (≠ V159) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
- A237 (= A163) to S: 65% of activity; dbSNP:rs62621664
- N255 (≠ T181) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
- G268 (vs. gap) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
- GSTTTG 284:289 (≠ GSEPAG 206:211) binding NAD(+)
- R334 (≠ M256) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- N335 (= N257) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
- C340 (= C262) modified: Disulfide link with 342, In inhibited form
- C342 (≠ A264) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
- N372 (≠ D293) natural variant: N -> S
- P382 (= P303) to L: in SSADHD; 2% of activity
- V406 (≠ I327) to I: in dbSNP:rs143741652
- G409 (= G330) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
- S498 (= S419) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
Sites not aligning to the query:
- 36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
- 93 C → F: in SSADHD; 3% of activity; dbSNP:rs765561257
- 533 G → R: in SSADHD; <1% of activity; dbSNP:rs72552284
2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
34% identity, 91% coverage: 39:451/454 of query aligns to 62:480/485 of 2w8rA
2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
34% identity, 91% coverage: 39:451/454 of query aligns to 62:480/485 of 2w8qA
P49189 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABALDH; Aldehyde dehydrogenase E3 isozyme; Aldehyde dehydrogenase family 9 member A1; Formaldehyde dehydrogenase; Gamma-aminobutyraldehyde dehydrogenase; R-aminobutyraldehyde dehydrogenase; EC 1.2.1.47; EC 1.2.1.3; EC 1.2.1.46; EC 1.2.1.19 from Homo sapiens (Human) (see 2 papers)
30% identity, 99% coverage: 5:454/454 of query aligns to 31:486/494 of P49189
- C116 (= C91) to S: in allele ALDH9A1*2
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed; alternate
- 2 modified: N-acetylserine; in 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed
Query Sequence
>WP_014148057.1 NCBI__GCF_000968535.2:WP_014148057.1
MAFHAINPVDGSTILTLDNWEASRLSKAMDVAELAAGPWADTPIKKRCELMYKAAEVLRT
RQEELATIITQEMGKLIKESRGEIEKCAWVCEYYAEHGPGFLEDEYIETDAAKSLVAYQP
LGIILAVMPWNFPFWQVFRFAAPALVAGNTGLLKHASNVPQCAQAIESVFKEAGFPVGVF
TTLMIGSVQVRGVIDDPRVKAVTLTGSEPAGKSVAEAAGANIKKSVLELGGSDAFIVLDD
ADLELAVKQGVTSRYMNGGQSCIAAKRFIVVDAIADAFLEKFKAAVEDLRTGDPMSVDTT
LAPMARHDLRDELHKQVIESIEAGAKIVTGCAPEAGSGAYYQASIIDHVKPGMRAWHEEL
FGPVAIVIRAKNEDDAVSIANSSDFGLGGSVWTQSNARGERVARQLQCGAAFVNGLVKSD
PRMPFGGIKRSGYGRELSHHGIREFVNAKTIWIK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory